Changes In Spatial Configuration Research Articles

Equilibrium configurations of line arrays with respect to the deviatoric mean drift forces

Monochromatic waves incident on an array of structures give rise to nonlinear, time-constant mean drift forces (MDFs). These forces depend on the array's spatial configuration; their magnitude and the direction is, in general, different for every structure in the array. If the spatial configuration of an array is not fixed, as is the case in arrays of individually anchor-moored structures, the time-constant differences in MDF on individual bodies can lead to a change in spatial configuration, which could, in turn, significantly affect both the first-order, time-harmonic response of the array, as well as the downwave component of the MDF. Here, we explore the dependency of these deviatoric forces on array configurations and on the frequency of the incident monochromatic waves. We consider configurations of line arrays (consisting of 2–5 vertical circular cylinders) that are described by 1 or 2 parameters, and we focus on the along-array component of deviatoric forces. Using multiple scattering computational simulations, we identify the array configurations in which the deviatoric drift forces are zero, and we discuss the stability of these equilibrium configurations with respect to class-preserving configuration perturbations. Both stable and unstable equilibria exist, but the relative number of unstable equilibria grows as the number of degrees of freedom of the configuration perturbations increases. Interestingly, the stable configurations experience a generally lower downwave mean drift force on the entire array than the unstable ones. Overall, the variations in the deviatoric and the downwave MDFs between equilibria are significant (on the order of the isolated body MDF).

Influence of Courtyard on the Behavioural, Cultural, Climatic and Spatial Characteristics in a Transformed Vernacular Courtyard House – A Case Study in Kumbakonam, Tamil Nadu

Background: Courtyard have been used in residential vernacular architecture for many decades. It was incorporated within the spatial design of a house for both its cultural and climatic significance. Objectives: To understand the significance of courtyard in modern-day context, this research aims to explore the behavioural, cultural, climatic and spatial characteristics of courtyard in a transformed vernacular house in Tamil Nadu. Methods: The vernacular house was documented. Unstructured interviews were conducted with the residents to understand the relationship of the courtyard and its surrounding spaces. A matrix of attributes was developed in the research. In-situ measurements were recorded in 2021 December (Winter) and 2022 May (Summer). A statistical analysis using ArcGIS software was carried out to evaluate the correlation between the attributes. Findings: Results indicated that the cultural links with climatic and spatial attributes have been either weakened or lost in a few cases after transformations. Cultural links with courtyard have been lost to a larger extent. The links between cultural attributes and spatio temporal activities have been lost in recent years. Spatio temporal activities are more linked to privacy in recent times. The relationship between courtyard and climatic attributes has been altered with the changes in spatial configuration. The links between the climatic and spatial attributes have become weak due to transformations but not lost. The courtyard is strongly linked to temperature compared to all other climatic attributes. Spatio temporal activities are more related to temperature and day lighting. These activities are associated with spatial attributes like size, enclosure and openings. Spatial attributes like enclosure and openings are more linked to courtyard. Novelty: The study establishes the relationship between the behavioural, cultural, climatic and spatial attributes of a residential courtyard. Keywords: Courtyard, Culture, Climate, Activity pattern, Transformation

Enhanced π-conjugation in hybridized local and charge transfer state by intramolecular hydrogen bonding to construct efficient red emitters for OLEDs and cellular imaging

Red emitting materials are the key to development of organic light-emitting diodes (OLEDs) and bioimaging, but limited by the energy gap law and aggregation-caused quenching (ACQ), impeding their applications. Herein, two red emitters TPA-QxPy and Cz-Ph-QxPy were designed and synthesized with aggregation-induced emission (AIE) characteristics. TPA-QxPy had a strong charge transfer (CT) state, while due to the different spatial configurations of the donor units, intramolecular hydrogen bonds were formed in the Cz-Ph-QxPy, enabling the locally excited (LE) state to be incorporated into CT emissive state to form hybridized local and charge-transfer (HLCT) state. The results demonstrated that the higher planarity of the Cz-Ph-QxPy enhanced the π-conjugation and hybridization between the CT and LE, showing a red emission at 600 nm and a high fluorescence quantum yield of (ΦPL) 30.8% in film. Cz-Ph-QxPy-based OLED achieved highly efficient red emission with external quantum efficiency (EQE) of 7.56% at 580 nm. Moreover, benefiting from AIE characteristics, the fabricated TQx NPs and CQx NPs with near-infrared (NIR)/red emission showed high photostability and biocompatibility and were successfully used for cellular imaging. This work provides new insights for promoting the luminescence performance of red emitting materials with HLCT state by spatial configuration changes.

Spatial Configuration Changes as a Form of Residential Adaptation in Urban Settlements in Indonesia: A Case Study of an Urban Settlement in Yogyakarta City Center in 1980 to 2020

Spatial Configuration Changes as a Form of Residential Adaptation in Urban Settlements in Indonesia: A Case Study of an Urban Settlement in Yogyakarta City Center in 1980 to 2020

Impact of landscape pattern change on water-related ecosystem services: Comprehensive analysis based on heterogeneity perspective

The transformation of surface landscapes by human activities is the main driving factor that affects changes in ecosystem functions and ecological processes, significantly influencing the ability of regional water ecosystems to deliver products and services to human society. However, the relationship between landscape heterogeneity and multiple water ecosystem services and the mechanisms of dynamic change remain unclear, posing challenges for managing and enhancing ecosystem services through landscape management decisions. Taking the Zishui basin as an example, this study aimed to clarify the impact of landscape heterogeneity on water-related ecosystem services and relationships by analyzing the relative importance and comprehensive impacts of landscape structure composition and landscape spatial configuration changes on three ecosystem services (water production, soil conservation, and water purification). These services were quantified for the years 2000, 2005, 2010, 2015, and 2018 using the InVEST model. Furthermore, quantitative indices were constructed to assess the impact of landscape heterogeneity on water ecosystem services. The results show that annual water yield and soil conservation increased at first and then decreased from 2000 to 2018, with 24.48% and 22.50% overall decreases, respectively. In contrast, the total export of N and P decreased at first and then increased, with 3.65% and 5.18% overall increases, respectively. The contribution indices of landscape pattern changes to water yield, soil conservation, nitrogen export, and phosphorus export were 0.04%–2.02%, 0.08 × 10−2%–0.32 × 10−2%, 13.57%–70.05%, and 11.01%–68.22%, respectively, showing interannual and spatial differences. The impact of landscape pattern change on water-related ecosystem services mainly reflects intensity change, which does not change the dominant relationships. Both landscape composition and configuration have impacts on water-related ecosystem services, but the effects of composition are larger than those of configuration. Among them, the proportions of cultivated land, woodland, and construction land, along with changes in Shannon’s diversity index (SHDI), patch density (PD), and edge density (ED) have obvious influences on water-related ecosystem services and show nonlinear relationships. In summary, the impacts of landscape heterogeneity indicators on water-related ecosystem services have obvious category differences, interannual changes, and spatial differences. This study provides new insight into the driving effects of landscape pattern change on the supply of water-related ecosystem services and will provide a reference for practical land management applications and policy formulation of water ecosystem services.

High Performance Near-Infrared Emitters with Methylated Triphenylamine and Thiadiazolo[3,4-g]quinoxaline-Based Fluorophores.

Three near-infrared emitters (2TPA-QBT, 2MeTPA-BT and TPA-QBT-MeTPA) were rationally designed and synthesized. Density functional theory (DFT) and time-dependent density functional theory (TDDFT) calculations showed that the introduction of mono- or di-methyl groups between the donors and acceptor could result in the spatial configuration changing greatly for 2MeTPA-QBT and TPA-QBT-MeTPA compared to their parent compound 2TPA-QBT. The emission of TPA-QBT-MeTPA had a more obvious hybridized local and charge transfer feature (HLCT) based on the influence of the steric hindrance of the methyl substituent. Attributed to their different spatial configurations and luminescence mechanisms, different emission wavelengths with photoluminescent quantum yields of 26%, 38% and 34% in toluene, as well as 24%, 27% and 31% in 4,4′-bis(N-carbazolyl)-1,1′-biphenyl (CBP) doped film, were observed for 2TPA-QBT, 2MeTPA-QBT and TPA-QBT-MeTPA, respectively. The constructed organic light-emitting devices (OLEDs) displayed electroluminescence with emission peaks at 728, 693 and 710 nm, with maximum external quantum efficiencies of 1.58%, 1.33% and 3.02% for the 2TPA-QBT, 2MeTPA-QBT and TPA-QBT-MeTPA-doped OLEDs, respectively. This work illustrated the effect of spatial configuration changes on the luminescence properties of donor-acceptor-type organic emitters.

The Habitat Amount Hypothesis implies negative effects of habitat fragmentation on species richness

AbstractThe habitat amount hypothesis (HAH) predicts that species richness in a habitat site increases with the amount of habitat in the ‘local landscape’ defined by an appropriate distance around the site, with no distinct effects of the size of the habitat patch in which the site is located. It has been stated that a consequence of the HAH, if supported, would be that it is unnecessary to consider habitat configuration to predict or manage biodiversity patterns, and that conservation strategies should focus on habitat amount regardless of fragmentation. Here, I assume that the HAH holds and apply the HAH predictions to all habitat sites over entire landscapes that have the same amount of habitat but differ in habitat configuration. By doing so, I show that the HAH actually implies clearly negative effects of habitat fragmentation, and of other spatial configuration changes, on species richness in all or many of the habitat sites in the landscape, and that these habitat configuration effects are distinct from those of habitat amount in the landscape. I further show that, contrary to current interpretations, the HAH is compatible with a steeper slope of the species–area relationship for fragmented than for continuous habitat, and with higher species richness for a single large patch than for several small patches with the same total area (SLOSS). This suggests the need to revise the ways in which the HAH has been interpreted and can be actually tested. The misinterpretation of the HAH has arisen from confounding and overlooking the differences in the spatial scales involved: the individual habitat site at which the HAH gives predictions, the local landscape around an individual site and the landscapes or regions (with multiple habitat sites and different local landscapes) that need to be analysed and managed. The HAH has been erroneously viewed as negating or diminishing the relevance of fragmentation effects, while it actually supports the importance of habitat configuration for biodiversity. I conclude that, even in the cases where the HAH holds, habitat fragmentation and configuration are important for understanding and managing species distributions in the landscape.

Contractile morning glory disk anomaly: analysis of the cyclic contractions and literature review

To investigate the contraction mechanism of morning glory disk anomaly using computer-assisted analysis of the cyclic contractions frame by frame and to review the literature on contractile morning glory disk anomaly cases. The fundus video of the patient under anesthesia was recorded using RetCam C300 lens. Frames at 1second intervals were extracted to quality-preserving TIFF images and manually selected areas of cup, disk, and pigmented ring were measured with image analysis software. Measurements were repeated twice, and estimated uncertainties were calculated. Two full-contraction phases and 1 half- and 1 full-dilation phase were recorded and analyzed. At the end of the second dilation phase, cup, disk, and pigmented ring were dilated to 93.7%, 97.6%, and 98.3% of their areas after first dilation. At the end of the second contraction cup, disk and pigmented ring areas remained 1.24, 1.01, and 1.01 times larger, respectively, than the areas at the end of the first contraction. Ectopic cholinergic muscle contraction alone is unlikely to explain the cyclic contraction dilation movements under constant stimulus. Potentially present smooth muscle cells can be provoked by the stretch caused by the influx of fluid into the subretinal space. Change in spatial configuration and oscillations with decreasing magnitudes can be explained by decreasing contractions due to decreasing stretch with decreasing fluid influx in each cycle after the initial triggering of outside pressure, causing the greatest pressure gradient and the greatest fluid influx.

The characteristics of face configural effect in illiterates and literates

Literacy acquisition can modulate the way we process visual words and language. However, little is known about its function in reshaping how we process non-linguistic materials, like faces. In this study, we explored this question by comparing the facial recognition skills of illiterate and literate adults in China. Our results showed that illiterates were less sensitive to changes in spatial configuration among key features in upright faces when stimuli were presented simultaneously. The differences in sensitivity of spatial configuration between the literates and illiterates were also observed in house processing. These results thus provide evidence that literacy acquisition during childhood could reshape configural processing.

Method to quantitatively assess electrode migration from medical images: Feasibility and application in patients with straight cochlear implant arrays

Objective: To propose a method for quantitative assessment of the migration of lateral-wall, straight electrode arrays after surgery based on postoperative Cone Beam Computed Tomography (CBCT) images and automated medical image analysis techniques.Methods: A preliminary study is conducted on 19 implanted ears. For each implantation, two CBCT images are objectively analyzed. Electrode arrays are consistently projected into the same coordinate system in order to estimate precisely the migration of each electrode. Spatial configuration changes are characterized with the overall curvature of the electrode array.Results: From the samples analyzed no significant electrode migration, extrusion or electrode curvature changes were found. Mean infinitesimal local migration reveals a tendency where apical electrodes tend to move away from the modiolus and basal electrodes away from the round window.Conclusion: CBCT images demonstrate adequate resolution with limited artifacts to assess the electrode array position in vivo. Automated medical image analysis techniques and consistent coordinate system allow to quantitatively estimate migration and extrusion effect for lateral-wall, straight electrode array.

Discrimination of changes in spatial configuration for multiple, simultaneously presented sounds.

Listeners discriminated changes in the spatial configuration of two-to-eight consonant-vowel (CV) stimuli spoken by different talkers, all simultaneously presented from different loudspeakers in various azimuthal spatial configurations. The number of CVs, spatial configuration of the sound sources, and similarity of the talkers speaking the CVs were varied. Experiment I used a same-different procedure to determine the discriminability of different spatial configurations of multiple sound sources. In experiment II, listeners determined the direction (clockwise or counterclockwise) of sound source rotation over eight rotational steps. In both experiments, performance declined as the number of sound sources increased beyond two.

Characterization and functional evaluation of oat protein isolate-Pleurotus ostreatus β-glucan conjugates formed via Maillard reaction

Oat protein isolate is nutritious but with poor processing functionality. Pleurotus ostreatus β-glucan with good processing functionality can be conjugated with oat protein isolate via Maillard reaction, leading to an improved utilization of protein in food industry. Therefore, we produced conjugate with oat protein isolate and Pleurotus ostreatus β-glucan via Maillard reaction under controlled dry-heating conditions. The formation of conjugates with high molecular weight was identified by a new band of sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The analysis of amino acid composition showed that cysteine and lysine were the dominant Maillard reaction sites of oat protein isolate and Pleurotus ostreatus β-glucan. Changes in spatial configuration of conjugates caused reduction in their surface hydrophobicity of proteins and intrinsic fluorescence intensity. Fourier transform infrared spectroscopy analysis of conjugates suggested that Maillard reaction could cause the C=O stretching vibration, as well as the C-H and N-H deformation vibration. Circular dichroism analysis indicated that the secondary structure of conjugates was altered by decreasing the contents of α-helix and β-sheet and increasing the contents of β-turn and random coil. The surface structure of conjugates was loose and porous using scanning electron microscope. Furthermore, Maillard reaction could improve the solubility, emulsifying property and thermal stability of oat protein isolate. Our findings confirm the potential of protein-carbohydrate conjugate formed by Maillard reaction, to improve the application of instable but valuable proteins in food industry.

Quantitative Investigation of the Role of Intra-/Intercellular Dynamics in Bacterial Quorum Sensing.

Bacteria utilize diffusible signals to regulate population density-dependent coordinated gene expression in a process called quorum sensing (QS). While the intracellular regulatory mechanisms of QS are well-understood, the effect of spatiotemporal changes in the population configuration on the sensitivity and robustness of the QS response remains largely unexplored. Using a microfluidic device, we quantitatively characterized the emergent behavior of a population of swimming E.coli bacteria engineered with the lux QS system and a GFP reporter. We show that the QS activation time follows a power law with respect to bacterial population density, but this trend is disrupted significantly by microscale variations in population configuration and genetic circuit noise. We then developed a computational model that integrates population dynamics with genetic circuit dynamics to enable accurate (less than 7% error) quantitation of the bacterial QS activation time. Through modeling and experimental analyses, we show that changes in spatial configuration of swimming bacteria can drastically alter the QS activation time, by up to 22%. The integrative model developed herein also enables examination of the performance robustness of synthetic circuits with respect to growth rate, circuit sensitivity, and the population's initial size and spatial structure. Our framework facilitates quantitative tuning of microbial systems performance through rational engineering of synthetic ribosomal binding sites. We have demonstrated this through modulation of QS activation time over an order of magnitude. Altogether, we conclude that predictive engineering of QS-based bacterial systems requires not only the precise temporal modulation of gene expression (intracellular dynamics) but also accounting for the spatiotemporal changes in population configuration (intercellular dynamics).

Adaptive Gaze Strategies for Locomotion with Constricted Visual Field.

In retinitis pigmentosa (RP), loss of peripheral visual field accounts for most difficulties encountered in visuo-motor coordination during locomotion. The purpose of this study was to accurately assess the impact of peripheral visual field loss on gaze strategies during locomotion, and identify compensatory mechanisms. Nine RP subjects presenting a central visual field limited to 10–25° in diameter, and nine healthy subjects were asked to walk in one of three directions—straight ahead to a visual target, leftward and rightward through a door frame, with or without obstacle on the way. Whole body kinematics were recorded by motion capture, and gaze direction in space was reconstructed using an eye-tracker. Changes in gaze strategies were identified in RP subjects, including extensive exploration prior to walking, frequent fixations of the ground (even knowing no obstacle was present), of door edges, essentially of the proximal one, of obstacle edge/corner, and alternating door edges fixations when approaching the door. This was associated with more frequent, sometimes larger rapid-eye-movements, larger movements, and forward tilting of the head. Despite the visual handicap, the trajectory geometry was identical between groups, with a small decrease in walking speed in RPs. These findings identify the adaptive changes in sensory-motor coordination, in order to ensure visual awareness of the surrounding, detect changes in spatial configuration, collect information for self-motion, update the postural reference frame, and update egocentric distances to environmental objects. They are of crucial importance for the design of optimized rehabilitation procedures.

Kajian Perubahan Ruang Terbuka pada Kawasan Bersejarah dengan Metode Space Syntax (Studi kasus Kawasan Kampung Kapitan Palembang)

The understanding of void in solid-void theory cannot be ignored since urban open space is, in fact, the connector between existing buildings. Here also social interaction takes place between people living around that urban open space. In historic areas urban open space is very important, not only as a place to socialize but also due to its ecological and spiritual meaning. The area of Kampung Kapitan is one of the historic areas in Palembang. However, developments in this area have ignored the aspect of conservation . By using space syntax analysis which can analyze changes in spatial configuration in a qualitative way, this paper proves that conservation of a heritage area not just focuses on its buildings, but also on the surrounding area, whih includes open space. This is signified so the sustainability of historic areas can be guarded in accordance with Law no. 11/2010.

Threshold effects of habitat fragmentation on fish diversity at landscapes scales.

Habitat fragmentation involves habitat loss concomitant with changes in spatial configuration, confounding mechanistic drivers of biodiversity change associated with habitat disturbance. Studies attempting to isolate the effects of altered habitat configuration on associated communities have reported variable results. This variability may be explained in part by the fragmentation threshold hypothesis, which predicts that the effects of habitat configuration may only manifest at low levels of remnant habitat area. To separate the effects of habitat area and configuration on biodiversity, we surveyed fish communities in seagrass landscapes spanning a range of total seagrass area (2-74% cover within 16 000-m2 landscapes) and spatial configurations (1-75 discrete patches). We also measured variation in fine-scale seagrass variables, which are known to affect faunal community composition and may covary with landscape-scale features. We found that species richness decreased and the community structure shifted with increasing patch number within the landscape, but only when seagrass area was low (<25% cover). This pattern was driven by an absence of epibenthic species in low-seagrass-area, highly patchy landscapes. Additional tests corroborated that low movement rates among patches may underlie loss of vulnerable taxa. Fine-scale seagrass biomass was generally unimportant in predicting fish community composition. As such, we present empirical support for the fragmentation threshold hypothesis and we suggest that poor matrix quality and low dispersal ability for sensitive taxa in our system may explain why our results support the hypothesis, while previous empirical work has largely failed to match predictions.

Stakeholders perceptions of local environmental changes as a tool for impact assessment in coastal zones

Through history, population growth and anthropic activities have pressed and affected marine environments, causing impacts that were not always studied or reported. In this context, evaluate stakeholders perceptions of a particular region in Coastal Zones (CZs) can be useful for identifying environmental impacts that occurred in the past, especially in the absence of preterit data and effective monitoring. Engaging stakeholders in the discussion of local transformations may also contribute to the development of shared local management strategies regarding the knowledge and opinions of stakeholders about the place they live in. Thus, considering Araçá Bay as a case of study, this research aimed to understand preterit and present transformations on the Bay, through the perception of the people who live and visit the region for a long period of time. Data collected with interviews enabled the identification of events and factors that have induced changes in the region, mainly related to large enterprises and buildings that occurred from the second half of the twentieth century. Major impacts perceived by interviewees were changes in spatial configuration of the Bay, changes in hydrodynamic and sedimentary patterns, reduction of coastal vegetation areas and increased pollution. Some of these changes were also pointed by scientific studies or observed in historic aerial photographs, and were no totally predicted by EIA of related enterprise. Considering the importance of communities' perception and its use to better understand historical facts, preterit and present impacts derived from local human interventions, it is concluded that they are an important qualitative database and can be useful for the development of management strategies and for EIA analysis.

Conservation and divergence of transcriptional coregulations between box C/D snoRNA and ribosomal protein genes in Ascomycota.

Coordinated assembly of the ribosome is essential for proper translational activity in eukaryotic cells. It is therefore critical to coordinate the expression of components of ribosomal programs with the cell's nutritional status. However, coordinating expression of these components is poorly understood. Here, by combining experimental and computational approaches, we systematically identified box C/D snoRNAs in four fission yeasts and found that the expression of box C/D snoRNA and ribosomal protein (RP) genes were orchestrated by a common Homol-D box, thereby ensuring a constant balance of these two genetic components. Interestingly, such transcriptional coregulations could be observed in most Ascomycota species and were mediated by different cis-regulatory elements. Via the reservation of cis elements, changes in spatial configuration, the substitution of cis elements, and gain or loss of cis elements, the regulatory networks of box C/D snoRNAs evolved to correspond with those of the RP genes, maintaining transcriptional coregulation between box C/D snoRNAs and RP genes. Our results indicate that coregulation via common cis elements is an important mechanism to coordinate expression of the RP and snoRNA genes, which ensures a constant balance of these two components.

Neuronal Activity Stimulated by Liquid Substrates Injection at Zusanli (ST36) Acupoint: The Possible Mechanism of Aquapuncture.

Aquapuncture is a modified acupuncture technique and it is generally accepted that it has a greater therapeutic effect than acupuncture because of the combination of the acupoint stimulation and the pharmacological effect of the drugs. However, to date, the mechanisms underlying the effects of aquapuncture remain unclear. We hypothesized that both the change in the local spatial configuration and the substrate stimulation of aquapuncture would activate neuronal signaling. Thus, bee venom, normal saline, and vitamins B1 and B12 were injected into a Zusanli (ST36) acupoint as substrate of aquapuncture, whereas a dry needle was inserted into ST36 as a control. After aquapuncture, activated neurons expressing Fos protein were mainly observed in the dorsal horn of the spinal cord in lumbar segments L3–5, with the distribution nearly identical among all groups. However, the bee venom injection induced significantly more Fos-expressing neurons than the other substrates. Based on these data, we suggest that changes in the spatial configuration of the acupoint activate neuronal signaling and that bee venom may further strengthen this neuronal activity. In conclusion, the mechanisms for the effects of aquapuncture appear to be the spatial configuration changes occurring within the acupoint and the ability of injected substrates to stimulate neuronal activity.

Homology modeling and molecular dynamics studies of Wilms’ tumor gene 1 frameshift mutations in exon 7

As a transcription factor, the Wilms' tumor 1 (WT1) gene plays an important role in leukemogenesis. The impact of WT1 gene mutations has been reported in acute myeloid leukemia (AML). However, the number of available studies on the spatial configuration changes following WT1 mutation is limited. In this study, we sequenced the mutation in exon 7 of the WT1 gene in 60 children with newly diagnosed AML and the spatial configuration of WT1 with frameshift mutations in exon 7 was evaluated using the software for homology modeling and optimization of molecular dynamics. Three cases with frameshift mutations in exon 7 were identified (3/60; mutation rate, 5%). One case had a mutation that had been previously described, whereas the remaining two mutations were first described in our study. Of the three cases, one case presented with antecedent myelodysplastic syndrome (MDS) and the remaining two cases exhibited primary resistance to induction chemotherapy. The spatial configuration analysis demonstrated that the three mutations affected the spatial structure of exon 7 and even affected exon 8 compared to its wild-type. This study demonstrated that the frameshift mutation in exon 7 of the WT1 gene is a poor prognostic factor for children with AML, partly through the spatial configuration changes following frameshift mutations of WT1, which highlights the structure-based function analysis and may facilitate the elucidation of the pathogenesis underlying WT1 gene mutations.