To compare the efficacy of the innervation zone-targeted injection technique (EUROMUSCULUS/USPRM spasticity approach) and the injection technique along the muscle length. A double-blind randomized controlled trial SETTING: Department of rehabilitation medicine of a medical center PARTICIPANTS: One hundred stroke patients with ankle plantar flexor spasticity. Modified Ashwort Scale, Modified Tardiue Scale, ankle range of motion measurement and 10 Meter Walk Test were used before and one month after injection. In addition to conventional rehabilitation, eligible patients were randomly assigned to two groups. The experimental group was injected with botulinum toxin along the length of the muscle, while the control group was injected with the same dose and volume of botulinum toxin 25-35% proximal to the medial head and 20-30% proximal to the lateral head of the gastrocnemius muscle. The study was completed by 60 participants with a mean age of 59.96±12.15 years. Both injection methods were found to be effective on range of motion, spasticity level, ambulation and walking speed. There was no statistically significant difference between injection methods CONCLUSION: Both injection methods of botulinum toxin A produce similar clinical effects.