A sector gate is a fundamental type of lock gate. It is designed as a large cantilevered, rotatable space truss structure capable of withstanding bidirectional hydraulic pressures and operating under dynamic water conditions. This makes it prevalent in medium- and large-scale navigation locks. This research examines the structural behavior of a ship lock sector gate using one in-situ test and two numerical models. The sector gate, with an 11.72-m span, consists of a panel beam system, space truss system, boundary column, and floating box. An in-situ test was conducted to obtain the stress and deformation of key components of the sector gate under positive hydraulic head difference conditions during regular operation. A finite element model was established, verified, and a model updating framework incorporating sensitivity analysis based on the Hooke-Jeeves direct search method was established. The updated model was employed to perform an in-depth examination of the gate's mechanical characteristics under extreme conditions of both positive and negative hydraulic head. The findings suggest that the most crucial region is situated at the intersection of the boundary column and the bottom struts. The stress and displacement responses of the components exhibit a positive correlation with water depth, with the struts and truss chords experiencing significant stress. The entire gate demonstrates twisting along the longitudinal axis of the lock chamber, which negatively impacts its ability to seal effectively. These findings provide valuable insights for the design of sector gates, contributing to the safe operation of gates and efficient navigation of waterways.