Soymilk Production Research Articles

Quantification of soluble protein content and characterization of protein secondary structure by Raman spectroscopy combined with chemometrics

In the large-scale industrialized production of soymilk, the accurate monitoring of protein content and its secondary structure is crucial to guarantee product quality and enhance nutritional value, while traditional protein detection methods have significant limitations. In this study, Raman spectroscopy was used to characterize the changes in the relative content of protein secondary structures during boiling, in which the α-helix structure was converted to other unstable secondary structures, indicating that the protein was thermally denatured. The study also accurately predicted the soluble protein content of soymilk for the first time by combining Raman spectroscopy and chemometrics based on algorithms such as Partial Least Squares (PLS), Least Squares Support Vector Machines (LSSVM), and Artificial Neural Networks (ANN). The results showed that the relative percentage deviation (RPD) values of all models were greater than 2, indicating that the models all possessed a certain degree of robustness; The PLS-based regression prediction model (with an average RPD value of 3.5592 and an average R2p of 0.9597) had the best overall prediction. The study's results can guide the soymilk production industry into online monitoring of soymilk quality.

Purification and characterization of α-Galactosidases from Penicillium griseoroseum for Efficient Soymilk Hydrolysis

Soybean utilization is limited by the presence of raffinose oligosaccharides (RFO), which are not digested by humans and cause gastrointestinal discomfort. This study explores the potential of α-galactosidases from Penicillium griseoroseum for RFO hydrolysis in soymilk. Two distinct α-galactosidase enzymes, designated α-Gal1 and α-Gal2, were purified using a combination of ion-exchange chromatography and native polyacrylamide gel electrophoresis. Both enzymes exhibited characteristics of multimeric proteins and displayed similar biochemical properties. Optimal activity was observed at a pH range of 4.5-5.0 and a temperature range of 40-45 °C. Notably, α-Gal1 demonstrated high thermostability with a half-life of 16 hours at 40 °C. The α-galactosidases displayed different substrate affinitiesfor the substrates ρ-NP-αGal, o-NP-αGal, rD-raffinose, D-stachyose, and mD-melibiose. The Michaelis-Menten constant (Km) values for α-Gal1 were 1.06, 1.31, 28.74, 19.88, and 4.77 mmol/L, respectively, while those for α-Gal2 were 0.8, 1.26, 30.46, 21.74 and 5.01 mmol/L, respectively. Both α-Gal1 and α-Gal2 were strongly inhibited by metal ions (Ag⁺, Cu2⁺, Fe2⁺, and Hg2⁺) and moderately inhibited by D-melibiose. Importantly, both enzymes efficiently hydrolyzed RFOs, achieving complete D-stachyose elimination from soymilk after a 6-hour incubation. These findings propose the promising application of these α-galactosidases in industrial soymilk production, potentially enhancing its nutritional value and alleviating gastrointestinal issues in consumers.

Accelerated measurement technology quantifying packaging light protection performance for soymilk nutrient and chemical stability

An accelerated in situ (AIS) light exposure and measurement technology for prediction of retail light protection performance of soymilk packaging is reported. The ability of a packaging material to protect light sensitive marker solutions, either comprising riboflavin (RF) or chlorophyll-a (CHLA), from an intense light source was determined. The light-induced decay of the marker was tracked with in situ spectroscopy and modeled to quantify the light protection performance of each packaging material. For a set of packages, chemical analyses (RF, thiobarbituric acid reactive substances (TBARS), hexanal) of packaged soymilk under Simulated Retail (SR) storage conditions demonstrated excellent correlation to the light protection performance data of the same package set determined by AIS (R2 > 0.97). The use of AIS enables rapid quantification of soymilk packaging material light protection performance and the associated preservation of soymilk nutrient and sensory properties, thus it may be applied to improve the design of soymilk packaging materials for light protection performance and contribute towards the improvement of packaged soymilk product shelf life.

Large-scale production of paraprobiotic soy milk in stirred tank bioreactor: A dual-step fermentation approach

This study investigates the feasibility of using soy milk as a substrate for cultivating Lactobacillus casei and its subsequent conversion into paraprobiotics. The research focuses on optimizing the fermentation process from shake flasks to bioreactor scale and assessing the antioxidant properties and proximate compositions of the resulting paraprobiotic soy milk beverage. The study highlights a dual-step large-scale fermentation process comprising an anaerobic fermentation step followed by a thermal inactivation step using the in situ temperature control system of the bioreactor. Results indicated that soy milk enriched with 10 g/L glucose and fermented at 37 °C for 24 h on flask scale provided optimal conditions for L. casei growth, achieving a bacterial count increase of 8.80 ± 0.11 log CFU/mL and a titratable acidity of 0.89 ± 0.03 %. These optimal conditions were employed for bioreactor scale fermentation, attaining a bacterial count of 10.02 ± 0.06 log CFU/mL and a titratable acidity of 1.07 ± 0.04 % at 24 h. The thermal inactivation step at 90 °C for 30 min successfully converted the probiotic soy milk into a paraprobiotic form, maintaining its antioxidant activity at 62.12 ± 2.58 % radical scavenging activity. The final paraprobiotic product met the Food and Drug Administration (FDA) specifications for titratable acidity, presenting a viable alternative for soy-based functional beverages.

Harnessing Fermented Soymilk Production by a Newly Isolated Pediococcus acidilactici F3 to Enhance Antioxidant Level with High Antimicrobial Activity against Food-Borne Pathogens during Co-Culture.

In this study, a newly isolated Pediococcus acidilactici F3 was used as probiotic starter for producing fermented soymilk to enhance antioxidant properties with high antimicrobial activity against food-borne pathogens. The objectives of this study were to investigate optimized fermentation parameters of soymilk for enhancing antioxidant property by P. acidilactici F3 and to assess the dynamic antimicrobial activity of the fermented soymilk during co-culturing against candidate food-borne pathogens. Based on central composite design (CCD) methodology, the maximum predicted percentage of antioxidant activity was 78.9% DPPH inhibition. After model validation by a 2D contour plot, more suitable optimum parameters were adjusted to be 2% (v/v) inoculum and 2.5 g/L glucose incubated at 30 °C for 18 h. These parameters could provide the comparable maximum percentage of antioxidant activity at 74.5 ± 1.2% DPPH inhibition, which was up to a 23% increase compared to that of non-fermented soymilk. During 20 days of storage at 4 °C, antioxidant activities and viable cells of the fermented soymilk were stable while phenolic and organic contents were slightly increased. Interestingly, the fermented soymilk completely inhibited food-borne pathogens, Salmonella Typhimurium ATCC 13311, and Escherichia coli ATCC 25922 during the co-culture incubation. Results showed that the soymilk fermented by P. acidilactici F3 may be one of the alternative functional foods enriched in probiotics, and the antioxidation and antimicrobial activities may retain nutritional values and provide health benefits to consumers with high confidence.

Nutritional value and fiber content of cookies prepared from okara flour substitution: study on dietary fiber, isoflavone, and chemical properties

Cookies as a popular and ready-to-eat snack began to be developed as an alternative food for people with special diets. Soy products are known to be a source of inexpensive protein, nutritious and have functional properties. Okara as a soybean residue from soymilk production has nutritional value such as protein, fat, antioxidant like isoflavone and others that can be applied to a variety of food such as cookies. The target of this study is to evaluate the fiber and nutritional value of cookies made from okara flour. Prepared cookies are analyzed for proximate, texture, antioxidant and fiber content. This study used ANOVA and followed with Honest Significants Difference (HSD) consisting 3 treatments (A0B0-without substitution of okara flour, A3B2-20% okara flour substitution and 0,5gram cinnamon powder and A4B2-30% okara flour substitution and 0,5gram cinnamon powder). The range of the cookie’s moisture content, ash, crude protein, fat, and carbohydrate was 2.16-4.01%, 1.39-1.65%, 9.1-17.09%, 17.20-21.07%, and 60.24-65.86% respectively. The highest fiber content (3.52%) and isoflavone level (47.4μg/mg) of cookies were cookies prepared with okara flour substitution 30%.

The effect of EM-4 dosage in fermentation on the quality of soy milk waste (SMW) as an alternative feed ingredient to increase production cost efficiency in the poultry business

The increasing consumption of soy milk, especially in European countries, has an impact on high soy milk production. This causes increasing the waste and it will have an impact on environmental pollution. This research aims to obtain the best dose of EM-4 in fermented soy milk waste (SMW) as an alternative feed to increase production cost efficiency in the poultry business. This research was conducted with laboratory experiments using a completely randomized design (CRD). This research used 5 levels of EM-4 (0; 2.5; 5; 7.5 and 10%) and each treatment was repeated 4 times. Statistical analysis showed that the EM-4 level was very significant (p<0.01) in increasing the quality of texture, moisture content, temperature, sugar and crude protein; reduce crude fiber; and significantly (p<0.05) improved SMW color. The average SMW texture score ranges from 1.00-3.00; moisture content (12.75-15.10%); temperature (27.68-29.33°C); sugar concentration (0.00-5.00%); crude protein (13.66-18.68%), crude fiber (5.07-9.67) and color score (2.50-3.75). It was concluded that fermentation using EM-4 as an inoculum could improve the quality of soy milk waste (SMW) as an alternative feed to increase production cost efficiency in the poultry business. The best quality of SMW was obtained in fermentation with an EM4 level of 7.5%

Effect of complex prebiotics on Lactobacillus gasseri JM1 fermented soymilk: Physicochemical, flavor characteristics, and metabolites

Fermented soymilk reduces its overall acceptability due to its unpleasant flavor, but synergistic fermentation of soymilk with prebiotics and probiotics may be an effective way to improve its flavor. In this study, soymilk was fermented by adding 3% complex prebiotics (fructose oligosaccharides (FOS): inulin: galactose oligosaccharides (GOS) = 2: 2: 1) in synergy with Lactobacillus gasseri JM1. The study found that by increasing the viscosity of soymilk (2528.80 ± 132.07 cP), which in turn increased its water holding capacity (52.07 ± 2.02%). Based on GC-MS, metabolomics, and multivariate statistical PCA analysis, 2,3-butanedione, 2,3-pentanedione, 2-butanone, and acetoin were found to be significant modifiers of soymilk flavor compared to the control group (P < 0.05). No odors such as 1-octen-3-ol, hexanal, etc. were detected. In addition, relatively high levels of isoflavonoids such as genistein and daidzein have also been found. This study provides ideas and baseline data for research related to soymilk product innovation, flavor extension, and quality improvement.

ЭКОНОМИЧЕСКОЕ ОБОСНОВАНИЕ ПРОЕКТА ПО ПРОИЗВОДСТВУ СОЕВОГО МОЛОКА

This article discusses a project for the production of soy milk in JSC Rassvet. The purpose of the economic justification of the soy milk production project for JSC Rassvet is to assess the prospects for the implementation of this proposal by calculating the investment base, determining the payback period due to the current economic situation and the effectiveness of the entire project as a whole. Since increasing the level of competitiveness and strengthening the position in the industry is closely interrelated with investment activity, we have developed and economically justified a soy milk production project. The relevance of this project is due to the fact that products from foreign manufacturers are currently being sold in local markets, since due to unfavorable circumstances, the Russian Federation has lost a dense network of sales channels and was subject to sanctions pressure, the state supports domestic entrepreneurs and promotes import substitution policy. Based on the above, it is worth noting that the development of such a project will allow you to take a stable position in the selected market segment, diversify the funds of JSC Rassvet and profit from the sale of alternative milk. Since this project for the production of soy milk provides for the creation of a new production line focused on the production of high-quality and environmentally friendly products, and in demand. Considering in this way, it should be known that the results of the study demonstrate high profitability and potential for further growth. The soy milk production project can be an important step in the strategic development of the enterprise and strengthen its position in the healthy food market.

Effect of Storage Conditions on Physical Properties, Lipid Oxidation, Isoflavones and Antioxidant Capacity of Flour Prepared from Soy Milk By-Product

During the production of soy milk, a by-product is produced, which is typically treated as a waste material. This by-product has significant levels of dietary fibre, proteins, isoflavones and antioxidant capacity. As such, it has been recommended as an effective functional ingredient when milled to a flour after drying at 100 °C. The shelf-life stability of this dried by-product is relatively unknown when stored under different storage conditions (2 °C, 20 °C and 40 °C) and initial moisture content (9%, 12% and 14%), both packaged and exposed to immediate environments. This study investigated the impact of storage over ten weeks on the physical properties, lipid oxidation, antioxidant capacity and stability of isoflavones of this functional ingredient. The results showed that exposure significantly affected the stability of flour, especially on moisture content, water activity, isoflavone concentration and lipid oxidation. Different initial moisture contents significantly affected the initial and final colour, alongside final moisture and water activity when stored covered at 2 °C and 20 °C. Different storage temperatures were found to affect the moisture content, water activity, lipid oxidation, conversion of isoflavones and antioxidant capacity, with all tested initial moisture contents. Of note, higher conversion rates of malonyl isoflavones to β-glucosides were observed at high temperatures and longer times, while a minimum change in aglycone content occurred. In conclusion, the stability of this flour is least influenced by the initial moisture content but is more affected by high storage temperature and unpackaged conditions.

Production of phenylalanine-reduced soymilk for phenylketonuria patients

Phenylketonuria (PKU) is a hereditary disease caused by the deficiency of phenylalanine (Phe) hydroxylase enzyme or its cofactor tetrahydrobiopterin. Treatment involves a Phe-restricted diet, although food options are limited. Soymilk, derived from soybeans, is a functional food with nutritional benefits. In this study, soymilk was produced and hydrolyzed with protease of Aspergillus oryzae and papain, and then activated carbon was used to remove Phe for PKU patients. The second-derivative spectrophotometry method was used to determine Phe content in soymilk. The results showed no significant difference in dry matter, fat, and crude fiber between soymilk and Phe-extracted soymilk with respect to the control group (P &lt; 0.05). Soymilk’s protein content was calculated as 2.74% (w/w) and that of Phe-reduced soymilk as 1.94% (w/w). Similarly, consecutive Phe content was 40.53 mg/L and 5.09 mg/L. After hydrolization, the calculated Phe removal rate was 87.44% (w/w), and the protein content was reduced by 29.19% (w/w). The study demonstrates that Phe-reduced soymilk is suitable for PKU patients, thus reducing the need for imported products and offering new market opportunities.

Few case studies as ideas for zero-waste from food production and processing

Globally, the amount of agricultural waste is huge but not properly utilized yet. Precisely, about one billion tons of food produced for human consumption is wasted each year. This wastage global economy costs are estimated at US$ 1 trillion on annual level. The definition of food waste varies globally depending on where food waste occurs in the food supply and consumption chain, how it is generated, and what it covers. This review is a compilation of few different approaches in the context of zero waste from food production and processing. Soybean hull exploitation due to mass production of tofu, soymilk, edamame, tofu sausages and burgers, soy breads, soy pasta and soymilk yoghurts and cheeses, animal feed leads to a formation of high waste load. There are several available ways for soybean hull valorization: as a biofertilizer, as a substrate for microbial growth, as an adsorbent, for extraction of antioxidants and in our case for extraction of enzymes. The global consumption of coffee is approximately 10 million tons, resulting in a substantial production of spent coffee grounds (SCG) worldwide. However, this by-product has recently gained attention as a valuable source of usable compounds, particularly in our case for essential oils. During the cheese production process, the whey obtained as a by-product can be used as a raw material for development of new dairy products and in the same time to prevent the potential pollution of natural watercourses. The valorisation of the waste whey obtained in the cheese production by using it in the development of new products also contributes in the environment protection. In N. Macedonia, about 1000 tons of blood waste from industrial slaughterhouses flow into natural watercourses every year and it can find application in various industries. These are just a few examples that show that the possibilities for the implementation of the zero-waste concept are unlimited.

Quality nutrition, metal content, and health risks in soy milk products using aluminum and stainless steel cookers

Improper processing of soymilk, such as soybean raw materials, use of well water, and use of cooking vessels, produces soymilk that does not meet SNI standards. These conditions can contaminate soymilk with harmful metals. The purpose of this study was to measure the nutritional quality and metal content of soymilk produced using aluminum and stainless steel cooking vessels. The research was descriptive and was conducted in July 2022. Analytical tests were carried out by comparing the nutritional content in the form of total protein, fat, lactose, and solid non-fat (SNF), and analyzing the heavy metal content. The results showed that the contents of Pb, Cu, and Hg in soy milk processed with stainless steel were lower than those of aluminum, from 11,494 ppm to 2,706 ppm, 114,612 ppm to 10,211 ppm, and 0,372 ppm to 0,012 ppm, respectively. The contents of Zn and As in soymilk with stainless steel were higher than those treated with aluminum, from 884,585 ppm to 73,616 ppm and from 0,107 ppm to 0,079 ppm, respectively. Cooking water also contributed 0,055; 0,044; 0,028, and 0,156 ppm to the heavy metal content of Pb, Cu, Zn, Hg, and As, respectively. In conclusion, the use of stainless steel in soymilk processing resulted in better nutritional quality and lower heavy metal content compared to aluminum.

KAJIAN PENGEMBANGAN AGROINDUSTRI PERTANIAN DALAM RANGKA PENINGKATAN PENDAPATAN ASLI DAERAH DI KABUPATEN KONAWE SELATAN

One of the government's strategic efforts in realizing increased economic development is development policy which should be based on strategic efforts to encourage an increase in Regional Original Income (PAD) through efforts to develop the role of industrialization in the agricultural sector in order to achieve successful regional economic development. The aim of this research is to determine and analyze the contribution of the development of agricultural agro-industry to society, and the development of Regional Original Income (PAD) as well as the development of the agricultural industry in increasing PAD in South Konawe Regency. This research uses quantitative and qualitative approaches. The data analysis method used is descriptive analysis. The research results show that the development of agricultural agro-industry in South Konawe Regency has made a significant contribution to society. This contribution can be in the form of producing various products such as tofu, tempeh, soy milk, banana chips, corn flakes, rice, and other products to meet community needs. Apart from that, the development of agricultural agro-industry has contributed as a source of income for the community, especially for those who work in agricultural agro-industry businesses. The development of agricultural agro-industry has made a significant contribution to development. It is good to see that the development of the number of business units is quite fast and opens up many new opportunities and jobs for the community, thereby reducing the level of open unemployment. The development of Regional Original Income (PAD) in South Konawe Regency fluctuates from year to year, which is greatly influenced by the regional revenue component from regional taxes, regional levies, separated regional wealth management results, etc. Apart from that, increasing PAD can be obtained through increasing people's income and purchasing power as well as increasing economic growth which can encourage the development of community economic activity so that it can encourage an increase in PAD.

Investigating thermal denaturation kinetics of 7S and 11S proteins and lipoxygenase in soymilk through ohmic heating

AbstractThe purpose of this study is to investigate denaturation kinetics of 7S and 11S proteins as well as lipoxygenase in soymilk during ohmic heating treatment. Because denaturation kinetics of 7S and 11S are nth‐order reaction, a reaction order was estimated based on experimental data and simulation results using Arrhenius parameters calculated through differential scanning calorimetry dynamic method. Denaturation kinetic of lipoxygenase was determined by denaturation ratio which estimated based on oxygenase activity of its after ohmic heating treatment at 65, 70, and 72.5°C. The reaction orders of 7S and 11S proteins during ohmic heating of raw soymilk were determined as 3.25 and 1.52, respectively; while the reaction order of 11S protein in preheated soymilk at 75°C for 1 h was found to be 1.94 with a good correlation between experimental data and simulation result. Electrophoresis results indicated that trypsin inhibitor might affect the denaturation of 11S protein during ohmic heating. The denaturation of lipoxygenase during ohmic heating followed a first‐order reaction at all temperatures, and denaturation simulation using the Arrhenius parameters corresponded well with the experimental data.Practical ApplicationsThis study provides valuable insights into improving a quality of soymilk products. 7S and 11S proteins play a critical role in determining viscosity of soymilk and tofu made from soymilk, which together account for a large proportion of the total proteins in soymilk. Understanding the thermal denaturation kinetics of 7S and 11S proteins can help food manufacturers optimize their processing parameters to achieve the desired physical properties of soymilk. Moreover, lipoxygenase is responsible for producing beany flavor components in soymilk, and understanding its thermal denaturation kinetic can lead to production of soymilk with high consumer acceptance. Our research supports a practical application of ohmic heating technology in the processing of soymilk.

Influence of Starch Content on the Sensory and Rheological Quality of Fermented Soy Milk

Fermented soy milk is depreciated by consumers for its poor consistency and vegetable aroma. To rectify this deficiency, starch of traditional sweet cassava variety, was used at different rates as thickening agent as well as four aromas (mint, strawberry, banana and pineapple), to enhance the taste in the production of fermented soy milk. The viscosity measurement was done with a Programmable BROOKFIELD DV III. The descriptive sensory analysis were carried out with 15 trained panelists. The hedonic test was conducted with 50 untrained consumers. The viscosity of fermented soy milk increase with the starch concentration. The fermented soy milk with 3% of starch has the highest viscosity with 813.4 cP than the control one with 207.4 cP. The fermented soy milk incorporated with 0.5, 1 and 1.5% starch are less consistent in the mouth. For thickness, samples with 1, 1.5 and 2% starch were judged moderately thick than 2.5 and 3% of starch. Panel appreciated the thickness, ropiness and mouth-coating character of fermented soy milk with 2 and 2.5% starch. The mint and strawberry aromas were the most appreciated. Fermented soy milk incorporate of 2, 2.5% of starch with mint and strawberry as aroma could be marketed.

Quantification of the environmental impact arising from the utilization of whole and defatted Okara in fermentative and dehydration processes

Okara is the insoluble pulp that remains after the grinding and filtration of soybeans during the production of soymilk and tofu. As it retains a noteworthy quantity of nutrients, there has been an increasing emphasis in the utilization of this residue for the development of sustainable processes. This study focused on assessing the environmental impact of employing okara as a medium for fermenting and dehydrating probiotic bacteria at laboratory scale. The evaluation was carried out using the Life Cycle Assessment (LCA) methodology, considering the entire process lifecycle. Whole okara and defatted okara were used as culture media for Lactiplantibacillus plantarum CIDCA 83114, followed by dehydration (either freeze-drying or spray-drying) and subsequent storage. For the purpose of comparison, both scenarios (whole and defatted okara) were evaluated using 1 kg of dehydrated final product for storage, as functional unit. Based on experimental results, the conservation of the received okara and the dehydration-storage (e.g., freezing and freeze-drying) phases were identified as the most significant environmental hotspots responsible for the most substantial impacts of the processes. The use of LCA facilitated the measurement of the environmental effects linked to the reutilization of okara as an agro-industrial residue, thus providing quantitative support when engineering its sustainable valorization.

Fermented soybean foods and diabetes.

The number of patients with type 2 diabetes mellitus is increasing, and its prevention and management are important. One of the factors contributing to the increased incidence of type 2 diabetes mellitus is the change in dietary habits, including a Westernized diet. Fermented foods are foods that are transformed by the action of microorganisms to produce beneficial effects in humans and have been consumed for thousands of years. The production and consumption of fermented soy foods, including natto, miso, douchi, cheonggukjang, doenjang, tempeh, and fermented soy milk, are widespread in Asian countries. This review focuses on fermented soybean foods and summarizes their effects on diabetes. Fermentation increases the content of ingredients originally contained in soybeans and adds new ingredients that are not present in the original soybeans. Recent studies have revealed that fermented soybean food modifies the gut microbiota-related metabolites by modifying dysbiosis. Furthermore, it has been reported that fermented soybean foods have antioxidant, anti-inflammatory, and anti-diabetic effects. In recent years, fermented foods, including fermented soybeans, have shown various beneficial effects. Therefore, it is necessary to continue focusing on the benefits and mechanisms of action of fermented foods.

The Effects of Okara Ratio and Particle Size on the Physical Properties and Consumer Acceptance of Tofu.

Okara, the solid byproduct of soymilk production, poses a sustainability concern, despite being rich in fiber and other healthful compounds. In this study, the physical properties of tofu made from soymilk fortified with differing levels of okara-either whole or fine (<180 µm)-and made with the traditional coagulant nigari were examined. The yield increased linearly with the okara concentration with values of 18.2-29.5% compared to 14.5% for the control. The initial moisture in the fortified samples was higher than the control (79.69-82.78% versus 76.78%), and both the expressible moisture and total moisture after compression were also greater in the fortified samples. With a few exceptions, the texture parameters did not differ between samples. Dynamic rheology showed that all samples had G' > G″. The storage moduli increased at different rates during each gelling step, with G' before and after gelling increasing with the fortification level, and was greater for the samples with fine particles than with whole particles. Consumer sensory panels using the hedonic scale showed traditional tofu had a slightly higher acceptability, but the panelists indicated they would be more willing to purchase okara-fortified tofu because of the health and sustainability benefits it might have. Thus, tofu could be produced with added okara with predictable but not profound changes in its physical properties.

Antioxidative Properties of Fermented Soymilk Using Lactiplantibacillus plantarum LP95.

In recent times, there has been a growing consumer interest in replacing animal foods with alternative plant-based products. Starting from this assumption, for its functional properties, soymilk fermented with lactic acid bacteria is gaining an important position in the food industry. In the present study, soymilk was fermented with Lactiplantibacillus plantarum LP95 at 37 °C, without the use of stabilizers as well as thickeners and acidity regulators. We evaluated the antioxidant capacity of fermented soymilk along with its enrichment in aglycone isoflavones. The conversion of isoflavone glucosides to aglycones (genistein, glycitein, and daidzein) was analyzed together with antioxidant activity (ABTS) measurements, lipid peroxidation measurements obtained by a thiobarbituric acid reactive substance (TBARS) assay, and apparent viscosity measurements. From these investigations, soymilk fermentation using Lp. plantarum LP95 as a starter significantly increased isoflavones' transformation to their aglycone forms. The content of daidzein, glycitein, and genistein increased after 24 h of fermentation, reaching levels of 48.45 ± 1.30, 5.10 ± 0.16, and 56.35 ± 1.02 μmol/100 g of dry weight, respectively. Furthermore, the antioxidant activity increased after 6 h with a reduction in MDA (malondialdehyde). The apparent viscosity was found to increase after 24 h of fermentation, while it slightly decreased, starting from 21 days of storage. Based on this evidence, Lp. plantarum LP95 appears to be a promising candidate as a starter for fermented soymilk production.