The North Tianshan Belt occupies a pivotal geological position within the southwestern Central Asian Orogenic Belt, making it an ideal location to unravel the long-term evolutionary history of the North Tianshan Ocean. However, how, and when the Tianshan oceanic basin was finally consumed and closed remains highly debated. To resolve these questions, here, we conducted a comprehensive investigation of the different litho-tectonic assemblages of the Dacaotan composite mélange belt in the North Tianshan Belt. Using detailed geological mapping, structural analyses, rock associations, and new geochemical and geochronological data, we divide the Dacaotan composite mélange belt into three litho-tectonic assemblages: (1) the southern sedimentary forearc (ca. 423–398 Ma) assemblage that is characterized by relatively coherent and well-bedded flysch sequences; (2) the central supra-subduction zone-type ophiolitic mélange assemblage (ca. 468–461 Ma) consisting of petrogenetically linked upper mantle and oceanic crustal components, which are exposed as tectonic slices or blocks within a serpentinite and sedimentary matrix; and (3) the northern accreted ocean plate stratigraphy mélange assemblage (ca.448–398 Ma) that is composed of accreted trench-fill turbidites, seamounts (OIB-like basic rocks) and ocean floor rocks (MORB-like basic rocks) dispersed in a sandy to pelitic matrix. Post-kinematic pluton and dyke reveal that the final accretionary event was completed prior to ca. 371 Ma. In conjunction with the spatial–temporal configurations of the different litho-tectonic assemblages, we conclude that the North Tianshan Ocean underwent bi-directional subduction and intra-oceanic subduction events during the Early Paleozoic. Our findings suggest the final consumption of the North Tianshan Ocean basin occurred before the Latest Devonian, resulting in the formation of the ultimate suture between the Central Tianshan Block and the North Tianshan Belt along the Kanggguertag-Dacantao composite mélange belt.