Cowpea Research Articles

Phenotypic characterization of different seed coat color of cowpea cultivars, Vigna unguiculata (L). Walp. in West-Central Côte d'Ivoire

Cowpea is a widely consumed legume in sub-Saharan Africa. Several cowpea cultivars based on seed coat color are widely observed. Consumers' choice of cultivar varies from one region to another. However, the lack of improved varieties and the limited availability of information on the productive potential of these cultivars is one of the constraints on the development of cowpea production. Thus, red, white, black and brown seed coat widely consumed were evaluated in the field over two consecutive years, 2022 and 2023 on a plot following a randomized complete block design with four replications. Morphological and agronomic parameters showed that there were two groups of cultivars with interesting agronomic characteristics. The first group composed of the black, brown and red cultivars had a relatively short reproductive time, averaging 66 days. These cultivars are considered early. The number of fruits of these cultivars was also high, with an average of 12 fruits per plant. The second group, consisting exclusively of the white seed coat cultivar produced high biomass and plant length. It had also twice the pod and seed weight of the other cultivars. It would be possible to develop hybrids in the future, seeking, for example, to improve the seed weight of the red cultivar with a short reproductive cycle by crossing with the white seed coat cultivar, with a view to creating an early variety with a high yield.

Characterising cowpea (Vigna unguiculata L. Walp) mini-core set for flowering responses to various photoperiod and temperature regimes

Characterising cowpea (Vigna unguiculata L. Walp) mini-core set for flowering responses to various photoperiod and temperature regimes

Exopolysaccharide is detrimental for the symbiotic performance of Sinorhizobium fredii HH103 mutants with a truncated lipopolysaccharide core.

The nitrogen-fixing rhizobia-legume symbiosis relies on a complex interchange of molecular signals between the two partners during the whole interaction. On the bacterial side, different surface polysaccharides, such as lipopolysaccharide (LPS) and exopolysaccharide (EPS), might play important roles for the success of the interaction. In a previous work we studied two Sinorhizobium fredii HH103 mutants affected in the rkpK and lpsL genes, which are responsible for the production of glucuronic acid and galacturonic acid, respectively. Both mutants produced an altered LPS, and the rkpK mutant, in addition, lacked EPS. These mutants were differently affected in symbiosis with Glycine max and Vigna unguiculata, with the lpsL mutant showing a stronger impairment than the rkpK mutant. In the present work we have further investigated the LPS structure and the symbiotic abilities of the HH103 lpsL and rkpK mutants. We demonstrate that both strains produce the same LPS, with a truncated core oligosaccharide devoid of uronic acids. We show that the symbiotic performance of the lpsL mutant with Macroptilium atropurpureum and Glycyrrhiza uralensis is worse than that of the rkpK mutant. Introduction of an exoA mutation (which avoids EPS production) in HH103 lpsL improved its symbiotic performance with G. max, M. atropurpureum, and G. uralensis to the level exhibited by HH103 rkpK, suggesting that the presence of EPS might hide the truncated LPS produced by the former mutant.

Physico-mechanical measurements, functional properties, proximate composition of newly developed climate-resilient cowpea (Vigna unguiculata) varieties and their classification with near-infrared spectroscopy

AbstractCowpea is an important staple in Ghana and many tropical and subtropicalregions. Improving food security and agricultural sustainability for these crops requires the development of climate-resilient varieties.This study aimed to classify five newly developed climate-resilient cowpea varieties—Bawutawuta, SAMPEA 14, SARI-tuya, IT13K-1070–2, and IT07K-299–6—using Near Infrared Spectroscopy (NIRS) for classification, while standard methods were used to evaluate their composition, functional and physico-mechanical properties. Proximate composition, including moisture, protein, ash, fat, crude fiber, and carbohydrates, was determined according to Association of Official Analytical Chemists methods. NIRS data was processed within the 950–1650 nm range to reduce spectral noise, employing Savitzky-Golay smoothing for baseline correction. Linear Discriminant Analysis facilitated multi-class classification, with cross-validation performed using a leave-one-sample-out method to evaluate recognition and prediction accuracy. The cowpea varieties exhibited significant differences in their chemical and physico-mechanical properties. Moisture content ranged from 7.21% to 10.88%, protein from 22.84% to 32.53%, ash from 0.90% to 1.81%, fat from 2.56% to 9.13%, crude fiber from 2.90% to 4.78%, and carbohydrates from 47.43% to 53.85%. NIRS analysis demonstrated high sensitivity, achieving a recognition accuracy of 98.08% and a prediction accuracy of 96.19%. Bawutawuta and SARI-tuya presented distinct spectral profiles, while SAMPEA 14, IT13K-1070–2, and IT07K-299–6 showed overlapping spectra. These findings provide valuable insights into the unique attributes of each cowpea variety, which can be leveraged to optimize agricultural practices, processing, and storage of these climate-resilient varieties.

Comparing quantum machine learning and classical machine learning for in vitro regeneration of cowpea (Vigna unguiculata)

Comparing quantum machine learning and classical machine learning for in vitro regeneration of cowpea (Vigna unguiculata)

A gamma rays-induced novel fertile subsessile leaf mutant in cowpea [Vigna unguiculata (L.) Walp

Protein-rich legumes contribute to nutritional security by complementing cereals-based diets rich in carbohydrates. In cowpea, an arid legume with a narrow genetic base, we attempted to induce ideotype mutations using gamma rays for augmenting productivity and discovered a novel fertile, subsessile leaf mutant ‘PLM211’. Despite the mutant and the parent exhibiting similar individual leaf area and node number, the rudimentary petioles, shortened rachis, little to no branching, and consequently fewer leaves greatly influenced the canopy structure of the mutant. Histological sections of the mutant’s 0.55- 1.01 cm short petioles showed reduced cell size and number as opposed to the parent’s 12.64-14.55 cm long petioles. The potential for uncovering the underlying genes associated with petiole length, leaf morphogenesis, and branching highlights the significance of the mutant as a genetic treasure, opening up avenues for manipulating plant architecture, especially in legumes.

Relationship between seed coat colour and seed vigour in cowpea (Vigna unguiculata Walp (L.)

In the present study, the 25 genotypes were grouped into two seed color groups including pigmented (n = 19; black, brown, dark brown, grey, dark grey seed color) and unpigmented (n = 6; cream and white seed color) for studying the association of seed coat color with seed vigor. Despite high germination (>84%) of all genotypes in the laboratory, unpigmented genotypes recorded low (34-54%) field emergence than pigmented genotypes (52-78%). Rapid initial (30 min) rate of water uptake (-0.75**), water uptake at one hour of imbibition (-0.70**), low proportion of seed coat (0.67**), and greater electrical conductivity of seed leachate (-0.72**) was associated with low field emergence in unpigmented cowpea genotypes. No significant correlation or association was observed between field emergence and laboratory germination in the present investigation suggesting that laboratory germination can not be used for predicting field emergence in cowpea. Instead, the electrical conductivity of seed leachate could be used to predict the field emergence of cowpea.

Enhancing the sustainability of cowpea production through the integrated use of fish effluents and animal manure

AbstractThe integration of aquaculture and agriculture in arid and semi‐arid environments is crucial for maximizing water and land productivity, especially considering the increasing global water scarcity and the simultaneous use of water for crop and fish production. A greenhouse study was conducted to determine the effects of fish effluent on the growth, yield parameters, and yield of cowpea (Vigna unguiculata). The experiment involved 13 fertilization treatments, including three types of irrigation water (river water—control, Nile tilapia (Oreochromis niloticus), African sharp‐toothed catfish (Clarias gariepinus), four fertilizers (poultry, cattle, and sheep manures at 10 t ha−1), recommended rate of inorganic fertilizer (150 kg ha−1 of NPK 6‐20‐10), and six mixed treatments with fish effluent and 50% of the applied rate of manure alone (5 t ha−1). The combined use of C. gariepinus effluent + 50% poultry manure significantly increased stem diameter, nodules per plant, pods per plant, and seed yield compared to NPK treatments. The shortest days to reach 50% flowering were obtained with the effluent of O. niloticus + 50% sheep manure, C. gariepinus/O. niloticus + 50% poultry manure, and 10 t ha−1 poultry manure. However, fertilization treatments did not significantly influence the number of branches, pod and root length, number of pods per plant, 100‐seed weight, and leaf chlorophyll concentrations. This study suggests that fish effluents, when combined with manure, can improve plant growth and seed yield, providing a cost‐effective alternative to inorganic fertilizers for smallholder farmers.

Growth, Yield and Grain Quality of Cowpea (Vigna unguiculata (L.) Walp.) and Weed Flora as Affected by Physical and Chemical Methods of Weed Control

Growth, Yield and Grain Quality of Cowpea (<scp><i>Vigna unguiculata</i></scp> (L.) Walp.) and Weed Flora as Affected by Physical and Chemical Methods of Weed Control

Symbiotic N2 fixation in cowpea varieties is markedly enhanced by inoculation with elite Bradyrhizobium strains

Because of its excellent ability to fix atmospheric nitrogen, cowpea [Vigna unguiculata (L.) Walp] makes a significant contribution to soil sustainability and productivity in the resource limited tropical regions. However, due to in part to ineffectiveness and limited availability of bio-inoculant, its symbiotic N contribution and yield remained low in the field. Therefore, this study examined the effect of elite cowpea infecting Bradyrhizobium strains (CP-24 and CP-37) on shoot biomass and symbiotic nitrogen contributions of four cowpea varieties (Keti, TVU, Black eye bean, and White wonderer trailing). For this a two-year field experiment was carried out at three sites using a factorial randomized complete block design with four replications. The natural abundance of the 15N technique was used to compute the symbiotic N contribution. Bradyrhizobium inoculation led to significantly higher nodule formation, % Ndfa, amounts of N fixed, and shoot biomass, demonstrating the effectiveness and ability of the strains to enhance soil fertility. Inoculating cowpea with CP-24 strain increased shoot N content, % Ndfa and N fixed by 40%, 15%, and 41%, respectively, in comparison to the un-inoculated control. Furthermore, the inoculant by variety interaction had a significant effect on nodule number, nodule dry weight, and amount of N fixed, with TVU and White Wonderer trailing in combination with CP-24 exhibiting the most outstanding performance. There was also a strong positive correlation between biomass accumulation and N fixed, as well as N fixed and seed yield. Therefore, Bradyrhizobium inoculation on cowpea varieties TVU and White Wonderer trailing with CP-24 strain is recommended at all three tested sites and similar agro-ecologies for improved symbiotic N contribution and associated yield advantage of cowpea. This study highlights that, the use of elite and crop specific Bradyrhizobium strains can boost symbiotic nitrogen contribution, soil fertility, and the yield performance of legumes. Thus, it helps resource-poor farmers who are suffering from rising mineral fertilizer cost to achieve food security while reducing climate change risks.

Contrast Relative Humidity Response of Diverse Cowpea (Vigna unguiculata (L.) Walp.) Genotypes: Deep Study Using RNAseq Approach.

Cowpea (Vigna unguiculata (L.) Walp.) is appreciated for its suitability for cultivation and obtaining good yields in relatively extreme farming conditions. It is resistant to high temperatures and drought. Moreover, food products prepared from Vigna are rich in many nutrients such as proteins, amino acids, carbohydrates, minerals, fiber, vitamins, and other bioactive compounds. However, in East and Southeast Asia, where the products of this crop are in demand, the climate is characterized by excessive humidity. Under these conditions, the vast majority of cowpea varieties tend to have indeterminate growth (elongated shoot length) and are unsuitable for mechanized harvesting. The molecular mechanisms for tolerance to high relative humidity remain the least studied in comparison with those for other abiotic stress factors (drought, heat, cold, flooding, etc.). The purpose of the work was to reveal and investigate differentially expressed genes in cowpea accessions having contrasting growth habits (determinate and indeterminate) under humid and drought conditions. We performed RNA-seq analysis using selected cowpea accessions from the VIR collection. Among the genotypes used, some have significant changes in their plant architecture in response to high relative humidity, while others were tolerant to these conditions. In total, we detected 1697 upregulated and 1933 downregulated genes. The results showed that phytohormone-related genes are involved in cowpea response to high relative humidity. DEGs associated with jasmonic acid signaling are proposed to be key contributors in the maintenance of compact architecture under humid conditions.

Evaluation of Different Genotypes of Cowpea (Vigna unguiculata L.) for Growth, Yield &amp; Quality under Prayagraj Agro-climatic Condition

The experiment was conducted at Department of Horticulture, Naini Agricultural Institute, SHUATS, Prayagraj during 2022-23 in order to standardize the best genotype of Cowpea. KASHI NIDHI (G8) found to be the earliest (3.0). The Cowpea genotypes KASHI NIDHI (G8) had noticed more yields per plant (587g), and per ha (168.03t/ha) under Allahabad agro climatic conditions. This variety also recorded desirable values for pod parameters like length of pod (32.53cm), weight of pod (110.07g), which are parameters deciding a better market acceptability. Thus, on the basis of growth characters, flowering behavior (36.87), and yield attributing characters, pod parameters and Cowpea genotypes KASHI NIDHI (G8) found to be promising. The highest profit and maximum benefit cost ratio (2.89) were observed in cowpea genotypes KASHI NIDHI (G8).

First report of lily mottle virus naturally infecting lily (Lilium asiatica) in Texas, USA.

Lily (Lilium asiatica) is an important plant grown for its range of flower colors and heavy scent. In March 2024, potyvirus-like symptoms consisting of light-yellow mottling and mosaic were noticed on 12/20 lily plants in a private property in Weslaco, Hidalgo County, Texas. Two symptomatic plants (WTX1 and WTX2) were sampled randomly for virus diagnosis. The leaf extracts of both samples were negative for the potyvirus group using Agdia's Poty ImmunoStrip® (Agdia, Inc., Elkhart, IN, USA). However, rub-inoculation of the extracts onto healthy Nicotiana benthamiana and Vigna unguiculata plants (n=4, each) induced mild mottle symptoms on the systemic leaves of both herbaceous test plants 20 to 28 days post inoculation, indicating the presence of a mechanically transmissible agent in the samples. No virus-like symptoms were observed on the mock-inoculated plants (n=1, each) of both species. To test for suspected potyvirus infection, 2-µg of total nucleic acid extracts (Dellaporta et al. 1983) from WTX1 and WTX2 were used for complimentary DNA (cDNA) synthesis with Oligo(dT) primer and the PrimeScript 1st strand cDNA synthesis kit (Takara Bio, USA). One microliter aliquot of each cDNA served as template in 12.5-µl conventional PCR reaction volumes with 5 U Taq polymerase (Roche Diagnostics, Indianapolis, IN), and two pairs of degenerate primers targeting the partial cylindrical inclusion (CI) gene and the helper component protease (HC-Pro) of potyviruses (Ha et al. 2008). The expected ~700-bp DNA product of each gene target was amplified from both samples. The amplicons were excised, gel eluted (Zymoclean™ Gel DNA Recovery kit) and cloned individually into the pJET1.2/Blunt vector (Life Technologies). Recombinant plasmids from two transformed Escherichia coli cells per cloned DNA insert were Sanger sequenced. BLASTn analysis of each gene-specific nucleotide (nt) sequence fragment revealed their identities (83 to 92 % nt) as lily mottle virus (LMoV; genus Potyvirus) at 93 to 98% query coverage. In pairwise comparison, the obtained sequences of LMoV isolates from TX specific to the CI (GenBank accession nos. PQ037260-61) and the HC-Pro (PQ037262-63) shared ~99% nt and 100% amino acid (aa) identities with each other and ~91-92% nt and 98-100% aa identities with the closest isolate, Bate5 (JN127341) from Australia. Based on these results, a pair of LMoV-specific primers (LM_v109-F: 5'-GGCCAGTAATGTGCACAAGC-3' and LM_c527-R: 5'-TCGCTGTAGCTAGCGACGTAC-3') was newly designed to target the partial CI gene, internal to the 700-bp fragment obtained above with the generic primers. The primer pair was used to screen each of the mechanically inoculated test plants by RT-PCR as previously described above. The expected 438-bp fragment of the LMoV CI gene was amplified from all the inoculated plants of both N. benthamiana and V. unguiculata; the results were confirmed by Sanger sequencing as described above. The mock-inoculated plant of each experimental host plant tested negative for LMoV. LMoV is a quarantine pest and was previously reported in the U.S. (Brierley and Smith, 1944) and other lily-producing parts of the world, such as the Netherlands, China, Korea, and Brazil. To our knowledge, this is the first confirmed report of the virus in Texas, thus expanding its geographical range. Testing of lily foundation stocks before propagation is essential to prevent further spread of LMoV via planting material.

Exploiting the synergistic influence of AgNPs-TiO2NPs: enhancing phytostabilization of pb and mitigating its toxicity in Vigna unguiculata

In this study, a composite of silver and titanium dioxide nanoparticles (AgNPs-TiO2NPs) was examined for its synergistic effects on phytostabilization of lead (Pb) and mitigation of toxicity in cowpea (Vigna unguiculata (L) Walp). Seeds of V. unguiculata were wetted with water, 0.05 and 0.1 mgL−1 Pb and 25 mgmL−1 each of AgNPs, TiO2NPs, and AgNPs-TiO2NPs. Root lengths of V. unguiculata were reduced by 25% and 44% at 0.05 and 0.1 mgL−1 Pb, respectively, while shoot lengths were reduced by 2% and 7%. In V. unguiculata, AgNPs and TiO2NPs significantly improved physiological indicators and mitigated Pb effects, with TiO2NPs modulating physiological parameters more effectively than AgNPs. The composite (AgNPs-TiO2NPs) synergistically regulated V. unguiculata physiology better than individual nanoparticles. Compared to individual AgNPs and TiO2NPs, the composite (AgNPs-TiO2NPs) synergistically increased antioxidant activity by 12% and 9%, and carotenoid contents by 88%. Additionally, AgNPs-TiO2NPs effectively reduced malondialdehyde levels by 29%, thereby mitigating the effects of Pb on V. unguiculata better than individual nanoparticles. AgNPs-TiO2NPs enhanced Pb immobilization by 57%, reducing its translocation from soil to shoots compared to V. unguiculata wetted with water. The bioconcentration and translocation factors of Pb indicate that phytostabilization was most effective when the composite was used.

Effect of Neem Oil in Combination with Propiconazole and Organic Amendments on Anthracnose (Colletotrichum lindemuthianum) of Cowpea (Vigna unguiculata L.)

The cowpea (Vigna unguiculata) is an annual herbaceous legume from the genus Vigna. Anthracnoses (Colletotrichum lindemuthianum) is a serious disease in cowpea growing areas and occurs as pre-harvest as well as post-harvest brown to black spots appear on the leaves. Neem oil at different concentrations (0.5%, 1%,1.5%, and 2%) along with Propiconazole were tested @ 0.1% in vivo during Kharif 2023 for their efficacy against disease incidence, plant growth and yield parameters of Cowpea at the Central Research Field of the Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj. The results of the present investigation revealed that it significantly reduced the disease incidence. Among the treatments the minimum disease intensity (%) at 75 DAS was recorded in T5 – Neem oil (2 ml/L) + Propiconazole 25 EC (0.1%) + VC@10gm/kg +SMC@10gm/kg (Foliar spray) was found to be significantly most effective treatment against Anthracnose (Colletotrichum lindemuthianum) on Cowpea and recorded minimum disease intensity (%) at 30, 45, 60 and 75 DAS, maximum plant height (cm), maximum number of branches, maximum yield (t/ha) and highest B:C ratio. The findings of the present experiment are limited to one crop season under Prayagraj agro-climatic conditions, as such to validate the present findings; more such experiments should be carried out in the future.

First report of Fusarium falciforme causing root and stem rot in cowpea (syn. black-eyed pea; Vigna unguiculata) in California.

In both April 2018 and September 2019, cowpeas / black-eyed peas (Vigna unguiculata) in one field in Tulare County, California were observed with tap root rot, both underground (foot) and aboveground stem rot, and in some cases canopy decline, compromising bean formation. In both fields, < 5% of plants appeared affected. Foot and stem segments (~1 cm) of 5-10 plants / field were disinfested sequentially with 0.1% Tween 20 (dip), 70% ethanol for 30 s, and 1% sodium hypochlorite for 2 min and placed on 1:10 potato dextrose agar with 0.03% tetracycline and Fusarium selective medium (Leslie and Summerell 2006). Fusarium-like isolates (dominant in isolation plates) were transferred to 0.6% KCl agar, where fusiform, curved macroconidia and varied microconidia in false heads on elongated monophialides were observed, characteristic of the Fusarium solani species complex (FSSC) (Leslie and Summerell 2006). Isolates CS221, CS222, and CS520 (representing different plants and locations) were saved as single hyphal tip cultures. An Illumina-derived genome sequence was assembled (Burkhardt et al. 2019) and partial tef1ɑ and rpb2 sequences (O'Donnell et al. 2022) were extracted from genome sequences in silico. Sequences were 99.9-100% identical to one another and to deposited F. falciforme isolates based on Fusarium ID and Fusarium MLST for tef1ɑ and rpb2, respectively (tef1a accessions: NRRL 28562 and NRRL 32331; rpb2 accession: NRRL 22857), and were deposited in GenBank (accessions in supplementary table). Pathogenicity was evaluated in three-week-old cowpea plants (cv. CB46rk2) in the greenhouse (13.5-33.6℃; 12:12 h L:D). The tap root / stem was wounded (1 mm wide, 2 mm deep) ~ 2 cm below the soil line and drenched with 50 ml of 106 spores / ml 0.1% water agar or with 0.1% water agar (negative control). The trial was arranged in a Randomized Complete Block Design with three blocks and 2-3 plants / isolate / block, and conducted twice. 52 d post-inoculation, below ground tap root / stem rot developed in 83% of F. falciforme-inoculated plants, with lesion lengths ranging from 25.2 ± 4.2 to 29.2 ± 8.0 mm (P = 0.893 for isolate, ANOVA). Canopy decline developed in 33-50% of plants across treatments in trial 1 (P = 0.859 for isolate) but not in trial 2, likely due to cooler conditions in trial 2 (January-March) vs. trial 1 (May-July), which were less stressful. F. falciforme isolates did not affect bean biomass (dry weight) vs. negative controls (12.5-14.8g / plant; P = 0.949 for pathogen treatment). FSSC isolates were recovered from 100% of symptomatic plants in the inoculated treatments but not in negative controls (both trials) and representative isolates from all treatments were confirmed as F. falciforme (tef1a analysis; trial 2 only). This study establishes F. falciforme as a root and stem rot pathogen of cowpea in California-a disease previously attributed to the morphologically and phylogenetically distinct F. phaseoli (syn. F. solani f. sp. phaseoli), but which lacked modern etiological studies (Frate et al. 2018; Geiser et al. 2021). This work is consistent with previous reports of F. falciforme as a root / stem rot pathogen in cowpea (Ajamu et al. 2023) and other beans (Sousa et al. 2017; Duarte et al. 2019). Clarification of disease etiology will improve accurate diagnosis and effective crop rotation-based management, since F. falciforme is also a pathogen of other California crops including melon, tomato and pistachio.

Effect of micronutrients applied foliarly on growth and yield of cowpea (Vigna unguiculata L.)

Effect of micronutrients applied foliarly on growth and yield of cowpea (Vigna unguiculata L.)

Effect of maize (zea mays l.) and cowpea (vigna unguiculata l.) intercropping on agronomic performance, yield and nutritional values of maize and cowpea under supplementary irrigation

A field experiment was conducted to evaluate the effect of maize (Zea mays L.) and cowpea (Vigna Unguiculata L.) intercropping on agronomic performance, yield, and nutritional values at Arba Minch University. Treatments were only maize crop (T1), only cowpea (T2), intercrop (maize and cowpea) in a 1:1 ratio (T3) and alternative rows of 2:2 (T4) as treatments. Treatments were arranged in a randomized complete block design (RCBD). Data on plant morphology, yield, nutritional composition, and economic return were recorded. The height of the plant (145.5 cm) of maize was significantly (P<0.001) higher at 1:1 intercropping than the height of the sole while the height of the cowpea was higher at the sole cropping. Higher number of branches (P <0.05) per plant (5.28) of cowpea was recorded in pure stands of cowpea than intercropping. The grain yield of maize (6.9 t/ha) and cowpea (4.2 t/ha) was significantly (P<0.05) higher in sole cropping than in intercropping. A higher dry matter yield of 9.06 t/ha of maize was obtained in a 1:1 maize- cowpea intercropping ratio than sole maize. Significantly (P<0.001) highest dry matter cowpea yield of 5.77 t/ha was obtained at sole cultivation than intercropping. The intercropping of maize with cowpea had an advantage of resource utilization in terms of land equivalent ratio (LER) 41.7% to 62.5%. The marginal rate of return (MRR) of intercropping in the ratio of 1:1 was as high as 48.23% in intercropping maize-cowpea, which was much better than sole cropping. Therefore, it could be recommended that the ratio of 1:1 maize-cowpea intercropping for land resource use and economic food and feed production in Arba Minch and similar agro-ecology zones.

The impact of foliar nutrition on the growth, yield characteristics, yield, quality parameters and economic viability of cowpea [Vigna unguiculata (L.) Walp.] cultivated under rainfed conditions

The impact of foliar nutrition on the growth, yield characteristics, yield, quality parameters and economic viability of cowpea [Vigna unguiculata (L.) Walp.] cultivated under rainfed conditions

Effect of sulphur and zinc fertilization on growth and yield of cowpea [Vigna unguiculata (L.) Walp.

Effect of sulphur and zinc fertilization on growth and yield of cowpea [Vigna unguiculata (L.) Walp.