Southeast Asia Research Articles

Analysis of Naja kaouthia snake venom composition and in-vitro enzymatic activities of 29 specimens in captivity: Highlighting the importance of individual variation in venom pool production

Naja kaouthia is a medically important snake, widely distributed throughout Southeast Asia, with a diverse venom composition. N. kaouthia venom is subject to significant intraspecific variation, caused by several factors, such as the wide geographic distribution of the species, sexual and ontogenetic factors. However, individual variation is a factor that has only been studied with small sample size groups and/or with pooled samples. With this in mind, this study evaluates the composition and in-vitro enzymatic activities of 29 individual venom samples from specimens born in captivity, with a similar genetic background caused by inbreeding, using SDS-PAGE under reducing conditions, RP-HPLC profiles and enzymatic activities of PLA2, LAAO and proteolytic activity over azocasein. Even in this scenario, we were able to observe significant variations in abundance and activity of PLA2. Individual variations in LAAO activity, as well as a sexual dimorphism in which males present a significantly higher LAAO activity than females were observed. Phosphodiasterase and CRiSP abundance were also found and considered to have multiple effects in the clinical manifestations of envenomation by presenting synergistic effects with other proteins from N. kaouthia venom. The RP-HPLC profiles were better at detecting compositional differences than SDS-PAGE profiles and better correlated with enzymatic activities, being a better technique to screen variation profiles and reinforcing the importance of individual venom analysis prior to pooling.

Murraya koenigii (L.) Spreng. as a Natural Intervention for Diabesity: A Review.

Murraya koenigii (L.) Spreng. (family: Rutaceae), commonly known as curry leaf or sweet neem, is a tropical plant native to India and Southeast Asia. It is highly valued in Ayurveda for its medicinal properties. Almost every part (fresh leaves, fruits, bark, and roots) of this plant is used to treat various ailments. Its fresh leaves are considered to have numerous medicinal properties for various diseases, including piles, inflammation, itching, fresh cuts, dysentery, and edema. A combination of curry leaf and buttermilk is used to treat diseases, such as amoebiasis, diabetes, and hepatitis. Its leaves are also believed to possess antioxidant, anti-inflammatory, and antimicrobial properties. The bark has been traditionally used for treating snakebites. Its roots are utilized in Ayurveda for the treatment of body aches. Being a storehouse of carbazole alkaloids, M. koenigii has been reported to show anti-obesity and anti-diabetic activity in in vitro and in vivo studies. The review aimed to appraise the role of M. koenigii leaf in the prevention of diabesity. We performed a literature search with the keywords "diabesity", "obesity", "diabetes", "adipose tissue", and "carbazole alkaloids" on Google Scholar, PubMed, and ScienceDirect databases. Several in vitro and in vivo studies conducted on cell lines and animals for anti-diabetic/anti-hyperglycemic and antihyperlipidemic activities have been included and appraised in the article, providing supporting evidence for the ethnomedicinal claims. This review has been an attempt to summarize comprehensively the overall research done on M. koenigii with regard to obesity and diabetes. The studies on anti-diabetic/anti-hyperglycemic and anti-hyperlipidemic activities of the plant have ranged from studies on crude extracts to isolated compounds. However, some of the studies require further in-depth analysis and validation of obtained results.

Incident cardiovascular disease risk among older Asian, Native Hawaiian and Pacific Islander liver cancer survivors

BackgroundCardiovascular disease (CVD) is a significant global health concern, particularly among Asian, Native Hawaiian, and Pacific Islander (ANHPI) communities that face unique health challenges. Liver cancer disproportionately affects ANHPI populations and has intricate associations with CVD risks due to shared pathophysiological mechanisms and metabolic disturbances. However, the specific CVD risk profile of ANHPI liver cancer patients remains poorly understood. MethodsUsing Surveillance, Epidemiology, and End Results (SEER)-Medicare data, we identified and matched 1150 ANHPI and 2070 Non-Hispanic White (NHW) liver cancer patients diagnosed between 2000 and 2017. We used the Fine-Gray sub-distribution hazard model to estimate hazard ratios (HRs) and 95 % confidence intervals (95 % CIs) for CVD risks, including ischemic heart disease (IHD), heart failure, and stroke, among ANHPI liver cancer patients compared to NHW counterparts and among ANHPI subgroups. ResultsANHPI liver cancer patients demonstrated a lower risk of IHD compared to NHW counterparts (HR, 0.65, 95 % CI, 0.50, 0.86), aligning with broader trends. Subgroup analysis revealed notable heterogeneity within ANHPI populations, with Southeast Asian (HR, 0.65, 95 % CI, 0.42, 1.00) and Chinese patients (HR, 0.53, 95 % CI, 0.33–0.83) exhibiting lower IHD risks compared to their NHW counterparts. However, Native Hawaiian and Pacific Islander liver cancer patients showed elevated risks of heart failure (HR, 3.16, 95 % CI, 1.35–7.39) and IHD (HR, 5.64, 95 % CI, 2.19–14.53) compared to their Chinese counterparts. ConclusionOur study highlights the complexity of CVD risks among ANHPI liver cancer patients. Addressing these disparities is crucial for improving cardiovascular outcomes and reducing the burden of CVD among ANHPI liver cancer patients.

Toward zero traffic deaths and disabilities with active and passive safety technologies in the association of southeast Asian nations

Road traffic crashes caused more than 108,000 deaths and 6,200,000 injuries resulting in 7.7 million disability-adjusted life years (DALYs) lost in the Association of Southeast Asian Nations (ASEAN) in 2019. This study estimates that 59 % of these casualties could have been avoided if all vehicles were designed to provide crash protection equivalent to that of the best vehicle safety design in their class and all road users adhered to safety best practices. Results of comparative risk assessments indicate that the application of safety technologies that are considered high priority by the United Nations (anti-lock braking, electronic stability control, occupant restraints, frontal and side airbags, crashworthiness, side-door beam, side structure and padding, and helmets) can save 34,373 lives and avert 2.5 million DALYs annually in ASEAN. While implementing Autonomous emergency braking and lane keeping assistance systems would provide additional reductions estimated at 13,077 fewer deaths and 1,021,220 fewer DALYs, speed-limitation systems would have a larger additional benefit, estimated at 21,394 lives saved and 1,382,530 fewer DALYs. The investigated technologies can be among the best approaches toward zero traffic deaths and can elevate public health burdens in low and middle-income countries.

A multi-gene phylogeny of the Asian kukri snakes (Oligodon Fitzinger, 1826): Sharpening the blade of the second largest serpent radiation (Reptilia: Squamata: Colubridae)

With 90 recognized species, kukri snakes in the genus Oligodon Fitzinger constitute the second largest snake radiation in the world. Oligodon species are collectively distributed across the Asian continent and possess several ecological and morphological attributes that are unique amongst other snakes. Despite their high levels of species richness, evolutionary relationships within Oligodon are poorly understood due to a limited number of samples and genetic markers available in earlier phylogenies. In this study, we assembled the largest molecular dataset of Oligodon to date, which we use to assess the systematics and biogeography of the entire genus. Based on a combination of maximum likelihood and Bayesian phylogenies using fragments of three mitochondrial genes (12 s, 16 s, CytB) and three nuclear genes (Rag1, C-mos, BDNF), we identify eight deeply divergent clades within Oligodon, of which only two correspond with species groupings that were recognized by previous morphological classifications. Four species delimitation methods employed on the mitochondrial portion of the dataset resulted in dramatically divergent estimations of molecular operational taxonomic units (mOTUs). When combined, all four methods support the existence of unrecognized species-level lineages, but also indicate that several other Oligodon species are poorly differentiated genetically and require additional integrative taxonomic research to properly resolve. Based on divergence dating, we demonstrate that Oligodon began to diversify during the early Neogene and hypothesize that the most recent common ancestor of the genus originated in mainland Southeast Asia. We conclude by recognizing eight phylogenetically defined species groups and identify sampling gaps that require further investigation once new data becomes available. This study contributes to a greater understanding of snake evolution on the Asian continent and acts as a baseline for future studies of this speciose genus.

Reconstructing suspended sediment concentrations in the Mekong River Basin via semi-supervised-based deep neural networks

The Mekong River Basin (MRB) is crucial for the livelihoods of over 60 million people across six Southeast Asian countries. Understanding long-term sediment changes is crucial for management and contingency plans, but the sediment concentration data in the MRB are extremely sporadic, making analysis challenging. This study focuses on reconstructing long-term suspended sediment concentration (SSC) data using a novel semi-supervised machine learning (ML) model. The key idea of this approach is to exploit abundant available hydroclimate data to reduce training overfitting rather than solely relying on sediment concentration data, thus enhancing the accuracy of the employed ML models. Extensive experiments on daily hydroclimate and SSC data obtained from 1979 to 2019 at the three main stations (i.e., Chiang Saen, Nong Khai, and Mukdahan) are conducted to demonstrate the superior performance of the proposed method compared to the state-of-the-art supervised techniques (i.e., Random Forest, XGBoost, CatBoost, MLP, CNN, and LSTM), and surpasses existing semi-supervised methods (i.e., CoReg, ⊓ Model, ICT, and Mean Teacher). This approach is the first semi-supervised method to reconstruct sediment data in the field and has the potential for broader application in other river systems.

Southeast Asian Rice and the Japanese Empire: The Transimperial Division of Labour and Capital Accumulation in the Global Food Regime

Southeast Asian Rice and the Japanese Empire: The Transimperial Division of Labour and Capital Accumulation in the Global Food Regime

Evidence of Vedic Sanātana Hinduism as a Global Dharma

This research explores the spread-out influence of Vedic Sanātana Hinduism, postulating that it once served as a foundational spiritual and cultural tradition with a reach that extended well beyond the Indian subcontinent. By looking into key aspects of Vedic culture, such as meditation, ritual practices, and Ārcavigraha (deity worship) as expressions of the divine, this study presents a multifaceted view of Hinduism’s spread and adaptation across diverse ancient civilizations. Evidence of Hindu deities and symbols found globally in archaeological excavations—ranging from Southeast Asia, Europe to the Americas and Canada, —demonstrates that elements of Hindu spirituality were not confined to any one region. Rather, they appear to have permeated a variety of cultures and belief systems over time, often integrating with local customs and symbols. Moreover, this research examines Vedic Hinduism’s contributions to scientific knowledge, literature, governance, and technology, highlighting the sophistication and cultural advancement of early Hindu society. Ancient Hindu texts like the Mahabharata and the Vedas depict Bhāratavarṣa, a concept of a unified Earth or global empire under Vedic values, which is reflected in historical records describing interactions with regions beyond South Asia, including the Mleccha, Yavanas, and other international groups. Stories and references from these texts suggest a thriving network of trade, communication, and diplomacy between India and other parts of the ancient world, where Vedic influences may have taken root. Furthermore, the principles of Vedic literature continue to shape modern philosophy, science, ethics, and governance, underscoring the enduring relevance of Hindu thought across multiple fields. Further, Vedic texts trace humanity's origins to Rishi Kashyapa and his descendants, with references to various tribes and races like the Yavanas and Mleccha crossing oceans to establish their own cultures. The enduring legacy of Vedic philosophy in science, literature, ethics, and governance underscores its universal appeal and lasting impact on modern disciplines. This study demonstrates that Vedic Sanātana Hinduism, in its scope and influence, was more than a regional faith; it served as a global dharma, profoundly shaping the course of world history and spirituality. Through a combination of historical documentation, scriptural analysis, and contemporary scholarship, this research contends that Vedic Sanātana Hinduism not only shaped Indian civilization but also left an indelible mark on the religious, intellectual, and cultural landscapes of many regions across the globe. This convergence of ancient evidence and modern insight reinforces the idea that Vedic Sanātana Hinduism once operated as a truly global dharma, offering a comprehensive and profound framework for understanding the interconnectedness of human society.

Anemia in Reproductive Age Group Women Attending in General Health Check-up in the Tertiary Care Centre in Kathmandu, Nepal: A Descriptive Cross-sectional Study

Background: Anemia is common, particularly among women in Southeast Asia and other Asian countries. In reproductive-age women (15-49 years), increased demand without adequate supply often leads to deficiency. This study aimed to determine the prevalence of anemia in this group. Methods: A descriptive cross-sectional study was conducted among women of reproductive age (15-49 years) visiting Tribhuvan University Teaching Hospital August 1st 2024 to October 20th 2024, following ethical approval. Exclusions included those with chronic diseases, incomplete data, heavy menstrual loss, or pregnancy complications. A convenience sampling method was used, yielding 372 participants. Data were recorded in MS Excel and analyzed in SPSS. Results: Our study of 372 participants (mean age 35.39 ± 8.73 years) found that 22.8% were women aged 40-44 years. BMI analysis showed 43% were overweight, and 34.1% had normal BMI. Blood group distribution was 27.4% A+ and 28.8% B+, with A- being the least common (0.3%). Most participants (75.3%) were from the Hill region, and 71.8% were unmarried. Anemia prevalence was 24.46%, highest in the 45-49 age groups and lowest in the 20-24 groups. Anemia was more common in the underweight (44.44%) and very obese (50%) groups and most prevalent among Terai region women (35.59%). Conclusion: The study found a significant prevalence of anemia among women of reproductive age, with higher rates observed in specific age groups, BMI categories, and geographic regions. This highlights the need for targeted interventions to address anemia in vulnerable populations.

50th anniversary of expanded programme on immunization: Shaping the next 50 years in the WHO South-East Asia region

50th anniversary of expanded programme on immunization: Shaping the next 50 years in the WHO South-East Asia region

Vegetation disturbance and regrowth dynamics in shifting cultivation landscapes.

Shifting cultivation, an age-old agricultural practice, is a major factor in forest cover change across Southeast Asia, where repeated cycles of vegetation disturbance and regrowth lead to far-reaching environmental and socio-economic impacts. The present study aims to assess the spatio-temporal patterns of vegetation disturbance and regrowth caused by shifting cultivation in Tripura state of India, over the past three decades, utilizing temporal segmentation of time-series Landsat data. The study analyzed vegetation disturbance and regrowth patterns in a shifting cultivation landscape from 1991 to 2020 using normalized burn ratio trends through LandTrendr, validated by the TimeSync tool. Six land use and land cover classes, viz., forests and trees outside forests, rubber plantation, shifting cultivation, water bodies, agriculture, and settlements, were mapped with an accuracy of 83.08% using a random forest classifier. This classification enabled the identification of the effective study area, which included forest areas with shifting cultivation patches. The analysis revealed that 2533.96 km2 of the study area remained undisturbed, 568.43 km2 experienced low-magnitude disturbance, 1501.11 km2 were moderately disturbed, and 184.82 km2 were highly disturbed. The shifting cultivation cycles in the study area show considerable variation. Low-magnitude disturbance indicates a single slash-and-burn event in the past three decades, moderate-magnitude disturbance involves fallow periods of over 10 years, and high-magnitude disturbance occurs with fallow periods of less than 8 years. The study revealed a shift from traditional shifting cultivation to more permanent agricultural practices in parts of Tripura. In response to incentives for commercial crops, cultivators are increasingly adopting long-term cultivation, including the growing of pineapple, areca nut, and rubber, as well as intercropping with papaya, banana, lemon, tapioca, pepper, and ginger. These plots are, however, abandoned if the yields become economically unviable. The expansion of monoculture cultivation significantly reduces the available area for shifting cultivation, thereby compelling jhum cultivators to revisit their jhum patches more frequently. This study is a first-of-its-kind attempt in the Indian context and sheds light on changing patterns in an age-old agricultural practice.

An in-silico analysis decoding the differences between the viral proteins of the American and the South-East Asian strains of DENV-4

An <i>in-silico</i> analysis decoding the differences between the viral proteins of the American and the South-East Asian strains of DENV-4

Pendulum swings rather than paradigm shifts: Southeast Asia’s securitisations and the global war on terror

Abstract Two decades into the ‘war on terror’, attention is rapidly shifting away from terrorism. Increasing geopolitical competition between the US and China and Russia’s war in Ukraine prompted talk about a watershed moment in global politics marked by a return of great power competition. To what extent has this paradigm shift – from terrorism to ‘traditional’ considerations of military security from external invasion – taken place in Southeast Asia? Building on securitisation theory, this article argues that the war on terror did not mark a universal historical-political period as it is often presented. In Southeast Asia, so-called non-traditional threats such as terrorism have concerned states since their independence. Therefore, Southeast Asia continued to prioritise ‘traditional’ security threats alongside ‘non-traditional’ ones in what is commonly described as its comprehensive approach to security. Consequently, when the ‘return to geopolitics’ began influencing military doctrine and preparation amongst NATO countries, a similar shift was absent in Southeast Asia. We argue that the region has seen varied emphases on non-traditional versus traditional security threats but did not experience the paradigm shift suggested by the US-dominated security narrative. Southeast Asia’s comprehensive security constellation underscores the need for a more pluralistic and eclectic approach to the study of international relations.

Hypertension in Adults With Diabetes in Southeast Asia: A Systematic Review.

Diabetes is one of the most pressing health issues in the Southeast Asian region, and hypertension has been commonly reported as a comorbidity in adults with diabetes. This systematic review aimed to synthesize evidence on theprevalence and management of hypertension in adults with diabetes in Southeast Asian countries. A literature search was conducted in Ovid MEDLINE and Embase Classic + Embase from database inception until March 15, 2024. Studies were included if (1) they were conducted in Southeast Asian countries, (2) the study populations were adults with diabetes, and (3) there was information related to hypertension or blood pressure (BP) in the study results. Of the 7486 abstracts found, 90 studies qualified for this review. Most studies reported a hypertension prevalence of 70% or higher (ranging from 29.4% to 93.4%). Despite this high prevalence, a substantial proportion of these populations did not receive adequate BP control, with most studies indicating a control rate of less than 40%. There was limited evidence on the prescription of antihypertensive therapies and medication adherence. There was a lack of studies from 4 of the 11 countries in the region. This review highlights that BP control in adults with diabetes remains a significant challenge in Southeast Asia. Given the ongoing epidemiological transition, and the increasing older population in this region who are likely to accumulate multiple chronic conditions complicating medication strategies, this review highlights the urgent need to improve BP management in those with diabetes.

Should mathematicians worry with PISA and TIMSS math results?

Since the end of last century, international large-scale assessment studies have been providing systematic information about countries’ education results. The news they convey regarding European education are not at all reassuring and have worsened with school closures and the disruption due to the recent Covid-19 pandemic. The size of the education losses and the distance of most European countries to other areas of the world, namely South East Asia, are often overlooked. A simple statistical analysis shows most European students are about one to two school years behind their peers in most advanced countries and regions, and the gap is increasing. These facts will negatively impact European workforce, economic competitiveness, and development. They will most likely also negatively impact mathematics higher education and research in our continent. To recognise the problem is the first step to cope with it. [1]

Zoonotic Dirofilaria sp. "hongkongensis" in subcutaneous nodules from dogs and cats, Hong Kong SAR.

Dirofilaria sp. "hongkongensis" is a putative Dirofilaria species, initially identified in subcutaneous nodules in humans in Hong Kong and in other South and Southeast Asian regions. While it differs genetically from the better-known zoonotic species, Dirofilaria repens and Dirofilaria immitis, information on the lesions caused by Dirofilaria sp. "hongkongensis" in the hosts as well as on its biology is scarce. This study documents for the first time the presence of this filarioid nematode in subcutaneous nodules in dogs and cats in Hong Kong, where it was originally described in human patients, therefore providing evidence for the zoonotic nature of this parasite. Records of Veterinary Diagnostic Laboratory of City University of Hong Kong were searched between 2019 and 2024 for histological reports of possible filarioid-associated lesions. Tissue samples were collected by excisional surgical biopsy and processed with routine paraffin techniques. Selected slides were stained using various staining techniques [i.e., hematoxylin and eosin, periodic acid-Schiff (PAS), Grocott methenamine silver (GMS) or Ziehl-Neelsen (ZN) and Gram stain]. DNA from formalin-fixed paraffin-embedded tissue were extracted, submitted to conventional polymerase chain reaction (cPCR) and sequencing (i.e., cox1 and 12S rRNA genes) and phylogenetic analyzed. A total of five subcutaneous nodules from four cats and one from a dog with histopathology suggestive of filariosis were selected. The presence of Dirofilaria sp. "hongkongensis" was morphologically and molecularly confirmed in one dog and one cat. Both histopathological presentation and phylogenetic analysis enabled classification of this species close to D.repens and within the subgenus Nochtiella. In the remaining three cases, one showed histological evidence of aberrant nematode migration, while non-parasitic causes were identified in the other two. This study provides the first evidence of Dirofilaria sp. "hongkongensis" in subcutaneous nodules in cats and dogs. The histology of clinical lesions of this filarioid species herein described is closely related to those caused by D.repens. Overall, this species should be considered in differential diagnoses of subcutaneous lesions in both animals and humans in the region.

A review on ethnobotany, phytochemistry, and pharmacology of the genus Duhaldea DC

The botanical drugs of genus Duhaldea DC. have been traditionally utilized in folk medicine for the treatment of a wide array of illnesses, encompassing fractures, bone wounds, carbuncles and poisoning, bronchitis, bruises, giddy with hypertension of qi, and lung deficiency cough. The genus Duhaldea DC. comprises 15 species widespread in Central, East, and Southeast Asia and 7 species (2 endemic) in China. The review aims to provide a systematic overview of ethnopharmacology, phytochemistry, and pharmacology of Duhaldea DC. and to explore the future therapeutic potential and scientific potential of this genus. The data were systematically collected from books and scientific databases such as PubMed, Web of Science, Google Scholar, CNKI, and doctoral and master’s theses. To date, a total of 352 metabolites have been isolated from this genus, and terpenoids, flavonoids, phenylpropanoids, and inositol angelates are the primary contributors to the pharmacological activities of Duhaldea DC. The crude extracts and isolated phytochemical metabolites from this genus have been shown to exhibit various pharmacological activities, including anti-inflammatory, antimicrobial, anti-osteoporotic, anticancer, and antioxidant activities. Despite notable advancements in our understanding of the chemical constituents and pharmacological properties of Duhaldea DC., it is absolutely crucial to conduct additional research into the pharmacology and toxicology of these species to definitively ascertain their safety, efficacy, and quality.

Utilization of cement deep mixing pile for soft soil foundation: a malaysian case study

Cement deep mixing piles, as representatives of deep mixing technology, are mature and widely applied. However, effective application cases of cement deep mixing piles are relatively scarce in countries like Malaysia in Southeast Asia. In this paper, the application of cement deep mixing pile reinforcement in Malaysia is presented. Solidifying material was selected for deep mixing piles based on the soil conditions using unconfined compressive strength (UCS) tests. The relationship between the strength of deep mixing piles on-site and soil properties (type, organic matter content, and ion content) was analyzed. And a quality evaluation of the deep mixing piles in the project is conducted based on Kolmogorov-Smirnov (K-S) tests and relevant standards. Results show that fly ash cement is suitable as a solidifying agent for acidic soil layers containing organic matter in the local area. The strength of piles is significantly related to the soil condition. The UCS of cement mixing piles in silty clay and peat soils is smaller than clay. The overall correlation between organic matter content in the soil and UCS is negative, particularly when the organic matter content ranges from 10% to 14%, resulting in a significant drop in UCS. The bilateral significance probability is greater than 0.05 in K-S test, indicating that the UCS results in the projectconform to a normal distribution. Additionally, the evaluation of the overall quality of the mixing piles aligns with the requirements in the Japanese standard “Manual of Deep Mixing Construction Technology for Harbors and Airports” and the Chinese standard “Technical Code for Building Foundation Detection”.

Identification and Characterization of Linear Epitopes of Monoclonal Antibodies Against African Horse Sickness Virus Serotype 1 VP2 Protein

African horse sickness (AHS) is an acute, fatal, contagious disease of animals of the family Equidae and is caused by infection with the African horse sickness virus (AHSV). Based on the outer capsid protein VP2, AHSV is classified into nine serotypes (AHSV−1 to −9) with little or no serological cross-reactivity between them. In 2020, AHS outbreaks caused by AHSV−1 were reported in Thailand and Malaysia, marking the first occurrences of AHS in Southeast Asia. However, little is known about the antigenic profile of AHSV−1 VP2. In this study, a recombinant VP2 protein was expressed in Escherichia coli and used as an immunogen, and three monoclonal antibodies (mAbs), designated 7D11, 10A9, and 9E7, against AHSV−1 VP2, were generated. These three mAbs were then successfully used in IFA, WB, and ELISA for the detection of AHSV−1 VP2. Two overlapping linear epitopes, 670NEFDFE675 (E670–675) recognized by 9E7 and 670NEFDF674 (E670–674) recognized by 7D11 and 10A9, were identified through truncation of GST-fused VP2. Amino acid sequence alignment shows that the 670NEFDFE675 motif is completely conserved within AHSV−1 but is highly divergent in other AHSV serotypes. Our studies provide an important tool for basic research into AHSV−1 and for the diagnosis of AHSV−1.

Peran Association of South East Asian Nation (ASEAN) dalam Mengatasi Transnational Human Trafficking di Asia Tenggara

Introduction: ASEAN, as a regional organization in Southeast Asia, aims to maintain security and stability in the ASEAN region, including addressing human trafficking.Purposes of the Research: The purpose of this study is to analyze and understand ASEAN's role in handling Transnational Human Trafficking in Southeast Asia.Methods of the Research: This study uses a normative juridical method with primary, secondary, and tertiary legal materials.Results of the Research: The research findings indicate that ASEAN has institutions such as SOMTC and ASEAN-ACT, and legal instruments such as the ASEAN Declaration Against Human Trafficking in Persons, Particularly Women and Children, APA, and ACTIP. Despite the principle of non-intervention, which can be a hindrance in some aspects of addressing human trafficking, ASEAN, through these institutions and mechanisms, has carried out its role and responsibility to maintain the stability of the Southeast Asian region