South Australian Merino Sheep Research Articles

Keratin gene expression in Merino sheep with divergent wool growth

South Australian Merino sheep were selected on the basis of high or low estimated breeding values (EBV) for wool growth rate (W), but with similar bodyweight, follicle density, and mean fibre diameter. Differences in the level of expression of keratin genes were examined in the skin of these sheep to test the hypothesis that divergence in EBV for wool growth is related to the production of wool proteins differing in sulfur (S) content. Further, it was proposed that this differential gene expression would be most pronounced when the supply of S amino acids to the animal was increased. Sheep selected for high EBV (+W) produced more wool per day than low EBV sheep (–W) (on average 32.5 v. 17.7 g/day clean wool, respectively; P < 0.05) but the S content of the wool did not differ between selection groups (2.77% v. 2.87% S, respectively; P = 0.2). Expression of keratin genes including keratin-associated protein KAP 2 (a high S gene), KAP 4 (an ultra-high S gene), KAP 6 (a high glycine/tyrosine gene), and the intermediate filament gene K 2.10, did not differ significantly between +W and –W groups. KAP 2 and K 2.10 each accounted for approximately 5% of the variation in wool growth rate (WGR) but expression of none of the genes examined was significantly related to the S content of the fibre produced. This suggests that differential keratin gene expression was not the source of genetic divergence in WGR. Instead the latter likely reflects a combination of differential cellular rate and growth processes (e.g. rate of bulb cell production, hypertrophy of cortical cells), differences in the relative production of inner root sheath and fibre from the follicle bulb cell population, or differential nutrient uptake into the follicle.

Phenotypic and genetic parameters for lamb and hogget traits in a flock of South Australian Merino sheep

The heritability of and phenotypic and genetic correlations among weaning weight (WW). greasy fleece weight (GFW), scouring yield (YLD), clean fleece weight (CFW), fibre diameter (FD), crimps per inch (CR) and staple length (SL) were estimated in a flock consisting of a control and 2 selection lines of South Australian Merino sheep. The effects of type of rearing, age of dam and sex were also examined. Estimates were compared with those currently in use in WOOLPLAN (National Performance Recording Scheme for non-pedigreed sheep) and in other studies. In general, agreement between the present study and other estimates was good, but there were some exceptions, namely: (i) standard deviation of GFW and FD; (ii) 'adjustment factors' for day of birth, type of rearing and age of dam for GFW, CFW and liveweight traits; (iii) heritability of YLD; (iv) phenotypic correlations of GFW and CFW with FD, and between YLD and CFW and (v) genetic correlations of WW with GFW, CFW and FD. It is concluded that special attention should be given to these exceptions in order to decide whether alterations to current 'accepted' parameter values are justified.

Genetic studies of South Australian Merino sheep. 4. Genetic, phenotypic and environmental correlations between various wool and body traits

Genetic, phenotypic and environmental correlations were estimated between 23 quantitative and qualitative wool and body traits of South Australian Merino sheep. Clean fleece weight was significantly correlated genetically with greasy fleece weight (0.62), yield (0.47), staple length (0.41), crimps per inch (-0.34), secondary/primary follicle ratio (0.37) and skin thickness (0.39). No significant half-sib genetic correlation was found between clean fleece weight and fibre diameter and the dam-offspring correlation was quite low, although significant (0.16, P < 0.05). This indicates that selection for clean fleece weight should not lead to any great increase in fibre diameter. Skin thickness is discussed as a possible early indicator of clean fleece weight.

Genetic studies of South Australian Merino sheep. 3. Heritabilities of various wool and body traits

Heritabilities have been estimated for a large number of quantitative and qualitative wool and body traits recorded on two flocks of South Australian Merino sheep over a 12-year period. Data were unadjusted for fixed environmental effects and so the estimates are applicable to the heterogeneous populations found in most practical situations. Dam-offspring heritabilities of quantitative traits ranged from 0.15 for primary follicle number to 0.63 for body weight. Greasy and clean fleece weights, percentage clean yield, staple length, crimps per inch, fibre diameter, secondary and total follicle number, skin thickness, coefficient of variation of fibre diameter and secondary/primary follicle ratio had moderate to high heritabilities. Dam-offspring heritabilities of qualitative traits ranged from 0.12 for weather damage of the fleece to 0.75 for birthcoat. Total folds, face cover and hocks had high heritabilities, and wool character, type of staple formation and wool quality had moderate heritabilities. The main production traits (body weight, greasy fleece weight, yield, clean fleece weight, staple length, fibre diameter and total follicle number) were corrected for variation due to type of birth and age of dam and their heritabilities re-estimated. No change occurred in the half-sib heritabilities; dam-offspring heritabilities increased by an average of 0.05.

Genetic studies of South Australian Merino sheep. 2. Environmental effects on wool and body traits at 15-16 months of age

The effects of years, sex, type of birth (i.e. whether or not multiple birth) and age of dam on 26 wool and body traits of South Australian Merino sheep were estimated by least-squares analysis. Years and sex had highly significant effects on all traits. Of the main production traits, type of birth had a significant effect on body weight, greasy and clean fleece weight and total wool follicle number, while age of dam only had a significant effect on body weight and greasy fleece weight. The possible consequences of failing to correct body weight and greasy and clean fleece weights for type of birth and age of dam were considered and it was concluded that, although in some years correction of these traits may not be warranted, in other years correction may be necessary to prevent selection against fecundity and an increase in the generation interval. The main components contributing to the 6.0 % difference between fleece weights of singles and twins were surface area, total follicle number and wrinkle score. The difference between fleece weights of animals from older ewes and animals from maidens was only 1.5 %; surface area was the main contributor to this deviation.

Genetic studies of South Australian Merino sheep. I. Genetic change in fleece and body traits

During the years 1953-67 one flock of South Australian Merinos was selected on clean fleece weight (the Index flock) and another on the basis of visual appraisal (the Visual flock). A large number of qualitative and quantitative traits were recorded. Linear regressions of differences between flock means on time were estimated for each trait; not many of the regressions were significant. However, greasy and clean fleece weights of Index animals increased significantly faster than those of Visual animals, and body weights of Visual animals increased significantly faster than those of Index animals. Realized selection differentials were estimated for all traits. There were significant selection differentials for greasy and clean fleece weights and yield in the Index flock, and for body weight and greasy fleece weight in the Visual flock. The response to selection was estimated for all traits by using two methods; regression of the trait on an individual's generation number, and comparison of the deviation from the overall mean of the mean of the progeny of a 3+-year-old sire with the deviation of the progeny of the same sire as a 2+-year-old. The estimate of genetic change in body weight was higher and genetic changes in clean and greasy fleece weights lower in Index animals, which was in contrast with an earlier result. However, when the least-squares means of these traits were compared, it was concluded that the Index flock did, in fact, achieve significantly greater responses in greasy and clean fleece weights and a significantly lower response in body weight than the Visual flock. It was therefore considered that, in these particular experiments, both methods of estimating genetic change had serious limitations.

Congenital goitre in sheep: isolation of the iodoproteins which replace thyroglobulin.

Immunological and chromatographic studies of proteins from the congenital goitre of South Australian Merino sheep revealed that normal thyroglobulin is absent from the thyroid glands of these sheep. However, a thyroglobulin-immunoreactive iodoprotein was isolated by affinity chromatography on agarose gel to which antibody against thyroglobulin had been covalently bound. Sucrose-gradient ultracentrifugation indicated that this iodoprotein had a sedimentation coefficient of 8S and a molecular weight of approximately 175000. This iodoprotein is therefore about one quarter the size of normal thyroglobulin (19S; 660000) and is similar in size to the subunit of thyroglobulin (3-8S; 165000) although this has usually been described as non-iodinated except when derived by reductive fission. In addition the goitre extract contained iodoproteins which had the immunological properties of serum albumin and immunoglobulin G. Determination of the iodine and iodoamino acid content of the hydrolysed iodoproteins revealed that they contained iodothyronines which were able to contribute to the production of thyroid hormones although the total iodothyronine content of the goitrous gland was less than that of the normal sheep thyroid gland.

Studies on the congenitally goitrous sheep. Iodoproteins of the goitre.

1. Congenitally goitrous thyroid tissue was obtained from South Australian Merino sheep. Ultrastructural studies of the secretory cells in this tissue showed active cells of normal appearance, containing apical protein droplets. 2. (125)I-labelling in vivo of goitre tissue was used to investigate the iodoproteins, in which the major proportion of (125)I appeared in the cell protein fraction soluble in 0.9% sodium chloride (average 62% in goitres from untreated sheep). 3. Ammonium sulphate fractionation showed two clear peaks of iodoprotein precipitation, one at 35-40% saturation and the other at 50-55% saturation. Both iodoprotein fractions contained iodotyrosines and iodothyronines, which were identified chromatographically after enzymic hydrolysis of the protein. 4. Polyacrylamide-gel electrophoresis at pH9.4, at either 7.5 or 5.0% acrylamide concentration, was used to characterize the iodoproteins. Two major fractions were observed, the fastest-migrating fraction coincident with serum albumin, and a slower-migrating, less-well-defined zone. This fraction migrated in 7.5% acrylamide gel, which excluded normal thyroglobulin. 5. Density-gradient (10-40% sucrose) centrifugation was used to determine the approximate sedimentation coefficients of the iodoproteins, which showed major components at s(20,w) 8-9S and s(20,w)<5S. 6. Immunoprecipitation with rabbit anti-(sheep thyroglobulin) failed to sediment (125)I-labelled proteins from goitre extracts. 7. Ouchterlony-type double diffusion in agar plates demonstrated immunoprecipitation lines between rabbit anti-(sheep thyroglobulin) and both the concentrated goitre extract and its Sephadex G-200-excluded fraction, which were confluent with that obtained on reaction with purified normal thyroglobulin. 8. It was concluded that both major iodoprotein fractions were capable of supplying thyroid hormones to the animal, and that the fraction of s(20,w)<5S was iodinated serum albumin. As (125)I-labelled thyroglobulin was not detected in goitre tissue from untreated or thyroxine-treated animals, it was possible that the genetic defect causing goitre resulted in an abnormal thyroglobulin, incapable of being iodinated but immunologically reactive.

Response to partial selection on clean fleece weight in South Australian strong-wool Merino sheep

The associations between 10 production characters and fertility at the R blood group, haemoglobin, and transferrin loci have been examined in two flocks of South Australian Merino sheep. The production characters were birth weight, hogget body weight, hogget wrinkle score, greasy fleece weight, clean fleece weight, staple length, degree of crimp, fibre diameter, fleece density, and skin thickness. No evidence of any real association was found. The significant associations which were found are ascribed either to chance or to the impossibility of eliminating sire effects from the analysis.

Measurements of wool production and its physiological components in a group of South Australian Merino sheep.

Data for wool production, feed intake, and body weight were recorded for a group of 31 rams selected from the South Australian strong-woolled Merino flock at Roseworthy. Measurements were made during (a) grazing, and (b) ad libitum pen feeding. A locally developed faecal nitrogen method was used to estimate intakes at pasture. For the pen diet, which contained 15% crude protein, two measurements of apparent dry matter and nitrogen digestibility were made for each sheep. The repeatability of gross conversion efficiency of feed into wool under grazing versus pen feeding was highly significant, viz. r = +0.79. While the repeatability of wool production was also highly significant, viz. r = +0 77, the repeatability of feed intake was not significant in these data, viz. r = +0 12 In pens, voluntary feed intake was found to vary with body weight to the power of + 1 09, the 99% fiducial limits extending from +0.81 to + 1 37 and thus excluding fractional powers conventionally assigned to basal metabolic nee&amp;, e.g. +0.7. The physiological components of wool production were studied with the use of' the pen data. An identity was used which involved three terms, viz. gross conversion efficiency of feed into wool, gross feed intake per unit body weight, and body weight. Of these, gross conversion efficiency was the prime determinant of wool production. Gross feed intake per unit body weight was significantly associated with wool production (P&lt;0.05), while the relationship between wool production and body weight was not significant in these data. In the pen experiment 'the regression relationship between voluntary feed intake and body weight accounted for approximately one-half of the variation in voluntary feed intake. A negative association between body weight and efficiency was also revealed. However, the general significance of this latter finding is doubtful, particularly in view of the variable relationships recorded between body weight and clean fleece weight for rams in 5 years' data from the general Roseworthy flock. For the experimental group of 31 rams, substantial differences were recorded in individual apparent digestive efficiencies for both dry matter and nitrogen. The ranges of individual values, expressed as four standard deviations, were 9.2 and 12.2% respectively. However, these differences did not contribute to differences in gross conversion efficiency of feed into wool, which suggests that the latter resides in either general or special aspects of metabolism.