Soursop Leaf Research Articles

Soursop (Annona muricata L.) in Indonesia: Biological evaluation, student perspective and internet trends

The study provided an overview of soursop (Annona muricata L.) in Indonesia, covering taxonomy, phytochemicals, antimicrobial tests, college student perspectives, and internet trends. Taxonomy details were sourced from literature, and herbarium specimens were deposited in the herbaria. Phytochemical compounds were identified using a UV-Vis spectrophotometer, while antimicrobial activity was assessed through disc diffusion. Student perspectives were collected via questionnaires, and internet trends were tracked with Google Trends. Data on taxonomy, student perspectives, and internet trends were analyzed descriptively, while experimental data underwent Kruskal-Wallis testing. Soursop, originally from tropical America, was likely introduced to Indonesia before 1884 and is now widespread. Soursop leaf extract exhibited phenol (2554.22 mg/100g), flavonoid (5263.43 mg/100g), and tannin (1469.77 mg/100g) presence. The study demonstrated its potent antimicrobial properties at concentrations of 25%, 40%, 55%, and 70%, with a 6 mm growth inhibition zone. College students at Health Polytechnic of Denpasar identified 42 treatable diseases with soursop, with fidelity levels (FL) ranging from 2.7% to 48.65%. The highest FL was observed for gout. Leaf and fruit parts of soursop are commonly used in traditional medicine. The trend of internet searches regarding the utilization of soursop (January 2011–September 2023) peaked in January 2012 and then declined until 2023, with the majority of searches occurring in central-western Indonesia.

Evaluation of d 18 in ovo administration of soursop (Annona muricata) leaf extract into the air space on hatch performance and physiology of Noiler chicks

Efficient poultry production can be accomplished using combined technologies. A combination of in ovo and ethno-veterinary technologies can deliver significant benefits, including reduced labour and production costs. This study evaluated the effect of in ovo administration of soursop leaf extract (SLE) on the hatching performance and physiology of Noiler chicks. A total of 550 eggs were incubated, and 460 fertile eggs were randomly distributed into 4 groups with 3 replicates on the 18th d of incubation. The eggs were then injected with 0.75 µg SLE, 1.5 µg SLE, and saline solution (negative control) at a rate of 0.2 ml in the air space. The noninjected group served as the positive control. At the end of the hatching period, the various groups were evaluated for embryo mortality, hatchability, hatch duration, organ weight, serum biochemistry, and chick quality. The results showed no significant differences (P > 0.05) in embryonic mortality, hatchability, organ weight, total protein, albumin, globulin, aspartate aminotransferase, alanine aminotransferase, and glucose among the treatment groups. However, chick weight, chick quality, and serum triglyceride levels were significantly (P < 0.05) higher in the extract-injected group. Additionally, incubation and hatch times were significantly lower (P < 0.05) in the SLE group compared to the other groups. In ovo administration of soursop leaf extract resulted in reduced incubation duration, hatch time, and embryo mortality. In conclusion, the in ovo injection of SLE improved hatch performance and chick quality.

Additional effect of soursop leaf extract fermented through drinking water on joper chicken carcas

Additional effect of soursop leaf extract fermented through drinking water on joper chicken carcas

Pelatihan Kader Dalam Penanganan Hipertensi: Optimalisasi Terapi Daun Sirsak Sebagai Bentuk Konservasi Yang Berbasis Kearifan Lokal

Background As age increases, the body undergoes many changes, both in terms of physical and psychological. Striking changes are physical changes which often cause health problems are changes in the cardiovascular system. One of them is hypertension which often occurs in the elderly. Based on data from the Ciamis Regency Health Office, there are around 640 elderly people experiencing hypertension in Cipaku sub-district. Hypertension is a lifelong disease, so it needs to be treated and maintained so that blood pressure is always in a normal state. One of them is by using alternative therapy of soursop leaf decoction water which in the Ciamis area itself has many soursop trees. The object of this service is health cadres in the Ciamis district area as many as 30 cadres. The method of this service is to provide training to cadres about soursop leaf decoction water and its impact on blood pressure. The results showed that the cadres' knowledge became better after being given this training with a picture of knowledge with an increase of 26.0. Conclusions Soursop leaf decoction water can be used as an alternative therapy in maintaining normal blood pressure. therapy in maintaining normal blood pressure.

Potential of Soursop Leaf Extract as an Antioxidant in MCF-7 Cells

The frequency of breast cancer tends to increase. Malondialdehyde (MDA) is a marker of oxidative stress as an end product from the chain reaction of lipid peroxidation. The use of traditional medicine soursopleaf (Annona muricata L.) has been reported for a long time because of its bioactivity as an antioxidant. This study analyzes the relationship between MDA levels and glutathione enzymes in MCF-7 cells given the methanol extract of soursop leaves. The methanol extract of soursop leaves was carried out by infusion method. The methanol extract of soursop leaves was given to cancer cells at several doses with an incubation of 24 hours. The cytotoxic test was carried out using the MTT method. Measurement of MDA levels was carried out using the thiobarbituric acid reactive substance (TBARS/TBA) reactivity test method. GSH measurements used the colorimetric method. The results showed that the ethanol extracts of soursop leaves have cytotoxic activity in the MCF-7 breast cancer cell line with IC50 values of 23.96 ppm. Ethanol extract of soursop leaves increased levels of MDA inhibition and GSH level. Soursop leaf extract could increase MDA inhibition GSH level in human breast cancer cells MCF-7.

IDENTIFICATION OF COMPOUNDS IN SOURSOP LEAVES (Annona muricata L) AS BREAST ANTICANCER AGAINST ALFA ESTROGEN RECEPTORS BY MOLECULAR DOCKING

The incidence of breast cancer in Indonesia is steadily rising. Women have the highest incidence of breast cancer. Acetogenins are highly effective inhibitors of mitochondrial complex I or NADH dehydrogenase, making them a promising option for therapeutic creation against breast cancer. The plant known as Annona muricata, also referred to as the soursop leaf plant, is purported to possess the ability to suppress the activity of breast cancer. Hence, it is imperative to assess the chemical composition of soursop leaves on receptors by docking experiments. Research utilizing the active site on the alpha estrogen receptor has determined that the reticulin molecule, with a free energy of -8.14 kcal/mol, is a potential contender for breast cancer therapeutic medications. Reticulin exhibits the highest affinity for binding to the active site of estrogen receptor alpha. This chemical exhibits the capacity to impede the growth of breast cancer.

Soursop leaf extract and fractions protects against L-NAME-induced hypertension and hyperlipidemia.

Despite the high phenolic content of Annona muricata, little is known about its anti-hypertensive and antihyperlipidemic properties. This study evaluated the anti-hypertensive and antihyperlipidemic potential of A. muricata leaf extracts. Forty-two male Wistar rats were divided into seven groups of six animals each. N-nitro-L-arginine methyl ester (L-NAME) was used to induce hypertension and hyperlipidemia. Phytochemical screening of Annona muricata leaf extracts (AMLE) revealed the presence of saponins, alkaloids, flavonoids, tannins, coumarins, steroids, terpenoids, and phenols. Comparing the methanol extract with the ethyl acetate fraction, quantification revealed that the methanol extract contained more phenolics, flavonoids, and alkaloids. The AMLE rats significantly reduced triglycerides, total cholesterol, LDL, VLDL, atherogenic index, coronary risk index, and blood pressure. The significant decrease in GSH, catalase, SOD, GST, and oxidative stress markers (MDA, nitrites, and MPO) was reversed by AMLE in a dose-dependent manner. Also, the elevated serum levels of TNF-α and IL-1β in the hypertensive rats were attenuated in the treatment groups. This study suggests the potential ameliorative effects of Annona muricata leaf extracts against L-NAME-induced hypertension in rats. Notably, the study showed the antioxidant and anti-inflammatory properties of A. muricata leaf extracts, which is seen in its ability to attenuate oxidative stress and inflammatory cytokines in L-NAME-induced hypertensive rats. A. muricata extracts also decreased atherogenic risk and improved lipid profiles.

Pengenalan Olahan Herbal Daun Sirsak (Annona muricata L.) Untuk Membantu Pengobatan Penyakit Neoplasma

Soursop leaves have long been used as a traditional medicine that can inhibit the potential of cancer cells. The purpose of introducing herbal preparations of soursop leaves is to educate in the treatment of cancer or tumors using soursop leaves and to find out the content contained in soursop leaves. To make this soursop leaf preparation by boiling the boiled water will be used as herbal preparations. The method of implementing the introduction of soursop leaf preparations was carried out using a zoom meeting. The activity was accompanied by giving pre-test and post-test questionnaires with an average understanding of the pre-test percentage of 88% to 89.2% during the post test. From these results, it can be said that the webinar that has been carried out can provide benefits in the form of insight and knowledge to the community.

Identifying the Quality of Preserved Eggs Using Soursop Leaf Extract (Annona Muricata L.)

Identifying the Quality of Preserved Eggs Using Soursop Leaf Extract (Annona Muricata L.)

The Effect of Infusing Soursop Leaves (Annona muricata L.) on Vaginal Discharge in Women of Childbearing Age: A Randomized Clinical Trial at the Lolo Community Health Center, Kerinci Regency, Jambi, Indonesia

Introduction: Vaginal discharge (leukorrhea) is a common gynecological complaint among women of childbearing age. While physiological discharge is normal, pathological discharge can be indicative of underlying infections or conditions. This study aimed to investigate the efficacy of soursop leaf infusion (Annona muricata L.) as a complementary treatment for vaginal discharge in women of childbearing age. Methods: A randomized controlled trial was conducted at the Lolo Community Health Center, Kerinci Regency, Jambi, Indonesia. Women aged 15-49 years presenting with vaginal discharge were randomly assigned to either the intervention group (soursop leaf infusion) or the control group (standard care). The intervention group received soursop leaf infusion twice daily for seven days, while the control group received standard care as per the health center's protocol. The primary outcome was the reduction in vaginal discharge symptoms (amount, color, odor, and associated discomfort) after seven days. Secondary outcomes included changes in vaginal pH and microbiological assessment. Results: A total of 130 women participated in the study (65 in each group). After seven days, the intervention group showed a significantly greater reduction in vaginal discharge symptoms compared to the control group (p &lt; 0.05). The amount, color, and odor of discharge improved significantly in the intervention group, along with a reduction in associated discomfort such as itching and irritation. Vaginal pH was also normalized in the intervention group, and microbiological assessment revealed a decrease in pathogenic microorganisms. Conclusion: Soursop leaf infusion (Annona muricata L) appears to be a safe and effective complementary treatment for vaginal discharge in women of childbearing age. Its antimicrobial and anti-inflammatory properties may contribute to its beneficial effects. Further research is warranted to explore its long-term efficacy and potential use in combination with conventional therapies.

The Effect of Giving Soursop Leaf Decoction to Hypertension Patients at the Elderly Social Home

Hypertension is a condition where a person experiences an abnormal increase in blood pressure in systole and diastole. There are many ways to lower blood pressure, one of which is with soursop leaf decoction water. The purpose of this study was to determine the effect of soursop leaf decoction water on lowering blood pressure in patients with hypertension at Harapan Harapan Kita nursing home, Palembang city. This study used a one group pre-test post-test pre-experimental design. The sample in this study were 33 people with hypertension in our hope nursing home Palembang city. With purposive sampling technique. This study was conducted for 8 weeks with 2 times the administration of Soursop leaf decoction water. The data analysis used is the Paired Sample T test. The instruments in this study used SOP (standard operating procedures) observation sheets for measuring blood pressure Sphygmomanometer, and stethoscope. The results of this study indicate that the administration of soursop leaf decoction water has an effect on systole blood pressure values with a P-Value = 0.000 and diastole blood pressure values P-Value = 0.001 so it can be concluded that there is an effect of soursop leaf decoction water on lowering blood pressure in elderly people with hypertension. The results of this study indicate that soursop leaf cooking water can reduce blood pressure in people with hypertension. Because of the content of antioxidants, potassium ions, which can lower blood pressure in soursop leaves.

Test the Effectiveness of Soursop Leaf Extract and Tapak Dara Leaf Extract as Mosquito Larvicide

In this rainy season the risk of contracting Dengue Fever increases. Many people control dengue fever chemically. The use of chemical larvicides has many disadvantages such as environmental pollution, resistance, and negative impacts on human health. This research aims to develop a natural larvicide from extracts of soursop leaves (Annona muricata) and tapak dara leaves (Catharanthus roseus). The study used a completely randomized design, with three replications. Soursop leaf and tapak dara leaf extracts were extracted using a maceration process using 70% ethanol solvent. III-IV instar Aedes aegypti mosquito larvae were used during the experiment, to assess the effect of formula (3 levels), dose (3 levels), and exposure time for 12 hours. All data were analyzed using Two-way ANOVA. The research results showed that the F1 formula and D3 dose were effective in eradicating mosquito larvae. Where the higher the concentration of soursop leaf extract and the higher the dose, the higher the mortality of mosquito larvae.

Cytotoxic activity of dichloromethane extract of soursop leaf (Annona muricata L.) against cancer

Cytotoxic activity of dichloromethane extract of soursop leaf (Annona muricata L.) against cancer

Food as Medicine: Soursop Leaf Tea in Melanoma Treatment and Prevention

Food as Medicine: Soursop Leaf Tea in Melanoma Treatment and Prevention

Pharmacological Activity of Plant Extracts in Self-Nanoemulsifying Drug Delivery System Dosage Form: Narrative Review

The utilization of medicinal plants as therapeutic agents has obstacles to achieving the target action due to their low solubility. Self-Nanoemulsifying Drug Delivery System (SNEDDS) is a method developed to increase the solubility of poorly water-soluble substances, such as medicinal plant extracts. This review aims to present a narrative overview of the potential utilization of SNEDDS in enhancing the pharmacological activity of plant extracts. Article searches were conducted on several websites, such as Google Scholar, PubMed, and ScienceDirect. Literature studies showed that the use of SNEDDS formulations in several medicinal plants such as dayak onion, soursop leaf, papaya leaf, bay leaf, kale, sidaguri, black cumin, mangosteen peel, harendong, pineapple peel, and cocoa is more effective than the administration of extracts alone because it can increase the solubility and bioavailability of a compound. Pharmacologically, SNEDDS shows its success in enhancing antioxidant, anti-inflammatory, analgesic, antidiabetic, antihyperglycemic, antimalarial, immunostimulant, antimicrobial, anticancer, wound healing, and hepatoprotective activities in various plant extracts. The utilization of SNEDDS formulations in plant-based drugs has a positive impact on the advancement of drug therapy, especially for compounds that have low solubility.

Analisis GC-MS dari Senyawa Bioaktif Ekstrak Etanol Daun Tanaman Sirsak (Annona muricata L.) sebagai Obat Tradisional

Soursop leaves (Annona muricata L.), have long been recognized in various parts of the world, especially in tropical regions, as a plant with various health benefits and traditional uses. The rich history of its use has been intertwined in various cultures, where the leaves are used not only as a food ingredient, but also as a herbal medicine. The phytochemical content in soursop leaves, such as acetogenin, alkaloids, and flavonoids has been the subject of intensive research in the last decade, highlighting their potential in treating a variety of health conditions, from inflammation to anti-cancer potential. The chemical structure and biological activity of these compounds have attracted the attention of researchers, especially in the search for alternative therapies for difficult-to-treat diseases. Soursop leaf extract has antioxidant, antimicrobial, antiparasitic, and antitumor properties that offer new insights into its therapeutic potential. This research method uses the maceration method, where soursop leaves will be extracted using ethanol solvent and then the compounds contained in the extract will be analyzed using Gas-Mass Spectrometry (GC-MS). The results of GC-MS analysis show that soursop leaves contain the compounds 2,3-Dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one, Trans-Caryophyllene, Hexadecanoic acid (CAS) Palmitic acid, 2-Hexadecen- 1-ol, 3,7,11,15-tetramethyl (Phytol), 9,12-Octadecadienoic acid (Z,Z) - (CAS) Linoleic acid, 9,12,15-Octadecadienoic acid methyl ester, Octadecanoic acid (CAS ) Stearic acid. The results of the analysis of compounds in soursop leaves can be a reference for further research to develop the potential of soursop fruit which leads to traditional medicine.

Case Report: Hypertension Accompanied by Obesity with Herbal Consumption of Soursop Leaf Boiling

Case Report: Hypertension Accompanied by Obesity with Herbal Consumption of Soursop Leaf Boiling

OPTIMIZATION OF SOURSOP LEAVES EXTRACT APPLICATION (ANNONA MURICATA L.) USING ENCAPCULATION WITH POWDERED MILK AS A HEALTH SUPPLEMENTS

Soursop leaves have untapped potential and are often considered waste. They contain beneficial compounds such as flavonoids, acetogenins, and polyphenols, which known to have an antioxidant and anti-cancer effects. However, these compounds are prone to degradation. In that case this study is aimed to optimize the encapsulation process of soursop leaf extract using variety of material formulations, temperatures, and drying time. The best condition was obtained with a ratio of 1:1.2 and a drying time of 120 minutes at a temperature of 55°C. Encapsulation enhances the stability of the extract, making it suitable for use as a health supplement. The encapsulation results were also analyzed using SEM-EDX to observe the structure of the encapsulated material and GC-MS to determine the composition of the extract. The major component detected in the extract was ketorolac, which accounted for 73.64% and 2.74% of the composition.

Uji Keamanan Hayati Bioinsektisida Nabati Ekstrak Daun Mahoni dan Sirsak terhadap Trichogramma SP. dan Menochilus Sexmaculatus

This study aimed to evaluate the biosecurity of botanical insecticides derived from mahogany leaf and soursop leaf extracts against natural enemies, the parasitoid Trichogramma SP. and the predator Menochilus sexmaculatus. The research employed a factorial completely randomized design with two factors: botanical insecticides at seven levels (P0:control, P1: mahogany leaf extract 20%, P2: soursop leaf extract 20%, P3: mahogany leaf extract 50%, P4: soursop leaf extract 50%, P5: mahogany leaf extract 10% + soursop leaf extract 10%, P6: mahogany leaf extract 25% + soursop leaf extract 25%) and natural enemies at two levels (parasitoid Trichogramma sp. and predator Menochilus sexmaculatus). The experiment ran for 4 days with observations made every 24 hours, totaling 28 experimental units with 4 replications. The results indicated that the highest biosecurity level against both Trichogramma sp. and Menochilus sexmaculatus was observed in treatment P1 (mahogany leaf extract 20%), with a mortality rate reaching 90%, while treatment P6 (mahogany leaf extract 25% + soursop leaf extract 25%) exhibited the lowest biosecurity level with a parasitoid mortality rate of 55%. Statistical analysis revealed significant differences between treatments P1 and P6. In conclusion, treatment P1 yielded significantly lower survival rates of the natural enemies Trichogramma sp. and Menochilus sexmaculatus compared to treatment P6 in terms of biosecurity testing. This study provides new insights into the development of botanical insecticides for effective and environmentally friendly pest management in agriculture. Keywords: bioinsecticide, botanical, natural enemies, survival rate

Pengaruh Proporsi Ekstrak Daun Kersen (Muntingia calabura Linn) dan Ekstrak Daun Sirsak (Annona muricata Linn) terhadap Aktivitas Antioksidan dan Daya Terima Yoghurt

Background: Indonesia ranks first with the most cancer cases in Southeast Asia. Degenerative diseases such as cancer occur due to the induction of free radicals, substances that can counteract and prevent damage caused by free radicals are called antioxidants. Objectives:To determine the effect of the proportions of kersen leaf extract and soursop leaf extract on the antioxidant activity and acceptability of yogurt. Methods: This research is experimental with a true experimental design (posttest with control group design). This research consists of 2 stages. The first stage, namely the introduction, aimed to determine the proportion of kersen leaf extract and soursop leaf extract that were most widely accepted/preferred by panelists from the 5 treatments given (P1 = 100 ml:0 ml, P2 = 75ml:25 ml, P3 = 50ml:50 ml, P4 = 25 ml:75 ml, P5 = 0ml:100 ml). The second stage or main research aims to determine the chemical quality (antioxidant activity) in the selected treatment. Results: The results of the organoleptic test showed that all hedonic categories, namely aroma, color and taste except for texture, had significant differences at p &lt;0.05. The best proportion was obtained in the P2 treatment, namely the proportion of 75ml DK : 25ml DS. The P2 treatment increased the antioxidant activity of yogurt, which was 52.33ppm compared to the control yogurt, which had an antioxidant activity of 73.78ppm. Conclusions: There is an effect of the proportion of kersen leaf extract and soursop leaf extract on the antioxidant activity and acceptability of yogurt except texture.