Sources Of Nitrogen Compounds Research Articles

Honey as Source of Nitrogen Compounds: Aromatic Amino Acids, Free Nucleosides and Their Derivatives.

The content of selected major nitrogen compounds including nucleosides and their derivatives was evaluated in 75 samples of seven varieties of honey (heather, buckwheat, black locust, goldenrod, canola, fir, linden) by targeted ultra-high performance liquid chromatography-diode array detector - high-resolution quadrupole time-of-flight mass spectrometry (UHPLC-DAD-QqTOF-MS) and determined by UHPLC-DAD. The honey samples contained nucleosides, nucleobases and their derivatives (adenine: 8.9 to 18.4 mg/kg, xanthine: 1.2 to 3.3 mg/kg, uridine: 17.5 to 51.2 mg/kg, guanosine: 2.0 to 4.1 mg/kg; mean amounts), aromatic amino acids (tyrosine: 7.8 to 263.9 mg/kg, phenylalanine: 9.5 to 64.1 mg/kg; mean amounts). The amounts of compounds significantly differed between some honey types. For example, canola honey contained a much lower amount of uridine (17.5 ± 3.9 mg/kg) than black locust where it was most abundant (51.2 ± 7.8 mg/kg). The presence of free nucleosides and nucleobases in different honey varieties is reported first time and supports previous findings on medicinal activities of honey reported in the literature as well as traditional therapy and may contribute for their explanation. This applies, e.g., to the topical application of honey in herpes infections, as well as its beneficial activity on cognitive functions as nootropic and neuroprotective, in neuralgia and is also important for the understanding of nutritional values of honey.

Bacterial cellulose synthesis by Kombucha microorganisms on a medium with a variable composition of nutrients

Bacterial cellulose synthesis by Kombucha microorganisms on a medium with a variable composition of nutrients. The paper presents the results of the assessment of the impact of various sucrose contents and the presence of various sources of nitrogen compounds in the growth medium of Kombucha microorganisms on the synthesis efficiency and the obtained bacterial cellulose mass. The analysis of obtained research results revealed that the efficiency of cellulose synthesis by Kombucha microorganisms depends on the quantity and quality of nutrients available in the growth medium.

Soils of Buffer Zones as a Source of Nitrogen Compounds

Soils of Buffer Zones as a Source of Nitrogen Compounds

Quality of Minas fresh cheese made with milk from F1 Holstein/Zebu cows fed diets with different sources of nitrogen compounds

ABSTRACT: This study aimed to evaluate yield, fatty acid profile, physical, chemical and sensory composition of Minas fresh cheese made with milk from cows fed diets containing different sources of nitrogen compounds (soybean meal, urea, sunflower meal and detoxified castor bean meal). Eight F1 Holstein/Zebu cows with average production of 20 kg milk corrected to 3.5% fat day-1 were distributed in two 4 × 4 Latin squares, consisting of four treatments (diets), four animals and four experimental periods. Cheese was produced on the last day of each experimental period. The physical and chemical composition, yield and texture of cheese were similar between diets. The used diets influenced the concentration of fatty acid C11:0, which was higher for diets with soybean meal and sunflower meal; C18:2 C9-T11 (CLA) was higher for diets with urea, soybean meal and sunflower meal and C20:3 for diets with soybean meal and urea. For the other saturated, monounsaturated and polyunsaturated fatty acids, differences were not detected. Different sources of nitrogen compounds in the diet for cows with average production of 20 kg milk corrected to 3.5% fat day-1 have no effect on the physical or chemical composition, yield, as well as acceptance of Minas fresh cheese. However, it can influence the fatty acid profile in the cheese fat.

COMPORTAMENTO INGESTIVO DE VACAS F1 HOLANDÊS X ZEBU ALIMENTADAS COM DIFERENTES FONTES DE COMPOSTOS NITROGENADOS

Resumo: Avaliou-se o comportamento ingestivo de vacas F1 Holandês x Zebu com 80 ± 12 dias de lactação e produção média de 20 ± 0,42 kg de leite/dia, alimentadas com diferentes fontes de compostos nitrogenados: farelos de soja, de girassol, de mamona desintoxicados e ureia. Utilizaram-se dois quadrados latinos 4 x 4, compostos de quatro animais e quatro dietas, com quatro períodos experimentais. O comportamento ingestivo foi obtido por avaliação visual para definir o tempo despendido em alimentação, ruminação, ócio e para avaliar mastigações merícicas e tempo de mastigação por bolo ruminal. Não houve diferença no tempo em alimentação (P = 0,1384), ruminação (P = 0,5964), ócio (P = 0,6314), número de períodos de alimentação (P = 0,5995), de ruminação (P = 0,4575) e de ócio (P = 0,4151), duração dos períodos de alimentação (P = 0,2711), ruminação (P = 0,8699) e ócio (P = 0,4025), em função da dieta utilizada. Não houve diferença na produção de leite (P = 0.2755) das vacas entre as dietas experimentais. A adição do farelo de girassol à dieta, em relação à utilização de ureia, resulta em menor consumo de FDN em minutos/kg (P = 0,0399) e consequentemente menor eficiência de alimentação da matéria seca (P = 0,0069), comparado ao tratamento com ureia. Assim, algumas características do comportamento ingestivo de vacas F1 Holandês x Zebu em lactação podem ser alteradas pelo uso de diferentes fontes nitrogenadas; entretanto, sem prejudicar a produção média de 20 kg de leite corrigida para 3,5% de gordura/dia.

Composição do leite de vacas alimentadas com diferentes fontes de compostos nitrogenados

ResumoObjetivou-se avaliar a composição físico-química e o perfil de ácidos graxos do leite de vacas alimentadas com diferentes fontes de compostos nitrogenados (farelo de soja, ureia, farelo de girassol e farelo de mamona detoxicado). O delineamento experimental foram dois quadrados latinos 4 x 4, sendo utilizadas oito vacas F1 Holandês/Zebu, com produção média de 20 Kg de leite com 3,5% de gordura dia-1. Amostras de leite foram coletadas e analisadas quanto à composição físico-química e perfil de ácidos graxos. Com exceção do nitrogênio ureico no leite, que foi maior na dieta com ureia, as demais variáveis da composição físico-química não foram influenciadas pelas dietas com diferentes fontes de compostos nitrogenados. Quanto ao perfil de ácido graxo houve variação nas concentrações dos ácidos C4:0; C18:0; C10:1; C12:1 e C18:1 T10 T11 T12. No entanto, para os totais de ácidos graxos saturados, monoinsaturados e poliinsaturados no leite, estes não diferiram entre as fontes de compostos nitrogenados. O uso de diferentes fontes de compostos nitrogenados na dieta de vacas F1 Holandês x Zebu, com produção média de 20Kg de leite com 3,5% de gordura dia-1, não altera a composição físico-química do leite, com exceção do nitrogênio uréico que aumenta na dieta com ureia. Entretanto, pode modificar o perfil de ácidos graxos da gordura do leite.

The fate of nitrogen compounds and heavy metals in studied semi-closed organic paddy fields

Abstract Agricultural development, cultivation management, and the application of fertilizer have affected the environment to such extent that organic farming has become an adopted trend to reduce the negative impact of all these factors. For a paddy field system, implemented activities in cultivation management are the essential factors that build up man-made materials such as nitrogen compounds and heavy metals. In this paper, we studied a non-polluted, low population density farming area, which is located within a semi-closed like valley where dry/wet deposition is the only natural source of nitrogen compounds and heavy metals. However this source can be ignored if it is compared to an applied organic fertilizer used in farming activities at the studied site. Cultivation management and field sampling in three organic paddy fields were conducted to investigate the outcome of organic farming. During the three-year study, concentrations of nitrogen compounds and heavy metals in the paddy fields, after a c...

Intake, digestibility and rumen dynamics of neutral detergent fibre in cattle fed low-quality tropical forage and supplemented with nitrogen and/or starch

The objective of this work was to evaluate the effects of nitrogenous compounds and/or starch supplementation on the intake, digestibility and rumen dynamics of neutral detergent fibre (NDF) in cattle fed low-quality tropical forage. Four crossbred heifers (Holstein x Zebu) with a body weight 231.9 +/- 15.5 kg and fitted with ruminal cannulae were used. The forage fed to the animals consisted of low-quality signal grass (Brachiaria decumbens Stapf.) hay, with an average crude protein (CP) level of 51.6 g/kg, on a dry matter (DM) basis. Four treatments were evaluated: control, without supplementation; supplementation with nitrogenous compounds (CP of the roughage was raised to 100 g/kg), on a DM basis; supplementation with starch at a ratio of 200 g/kg DM of roughage; and supplementation with nitrogenous compounds and starch as described above. A mixture of urea, ammonium sulphate and albumin was used as a source of nitrogenous compounds at a ratio of 4.5:0.5:1.0. The experiment was carried out according to a 4 x 4 Latin square design in a 2 x 2 factorial arrangement. There was a positive effect of the nitrogenous compound supplementation on the DM and NDF intake (P < 0.01). In contrast, starch supplementation decreased forage intake (P < 0.10). Nitrogen supplementation increased the digestibility coefficient of DM and NDF (P < 0.05). Supplementation with nitrogen and starch together increased the microbial assimilation of nitrogenous compounds in the rumen (P < 0.05). We observed that nitrogen supplementation increased the estimated weighted degradation rate of NDF by 14.8%, whilst starch supplementation decreased this rate by 32.5%.

The role of environmental factors and medium composition on bacteriocin-like inhibitory substances (BLIS) production by Enterococcus mundtii strains

Bacteriocin-like inhibitory substances (BLIS)-producers Enterococcus mundtii WGWT1-1A, WGW11.2, WGJ20.1, WGJ40.2 and WGK53 from raw material origin were subjected to a study for the characterization of antimicrobial compound production under several growth conditions, including different cultivation media, growth temperatures, pHs, different concentrations and sources of nitrogen compounds, carbohydrates and other nutritional factors, and in the presence of different percentages of ethanol and NaCl. The five E. mundtii strains showed different behaviors. However, in all cases, MRS and sour dough bacteria (SDB) were found as the optimal media for BLIS production. In general, the higher BLIS production was observed with pH in the range 6.0–8.0 and, except 45 °C, the temperature did not show a defining effect. Low or no BLIS activity was detected after growth without nitrogen sources and carbohydrates. Absence of Tween 80, triammoniun citrate, K 2HPO 4, MgSO 4 and MnSO 4 did not affect BLIS activity levels. Except for a strain (WGWT1-1A), ethanol did not play a negative role in BLIS expression, while NaCl determined decrease of BLIS activity, proportional with concentration. The above strains did not contain plasmids, hence, BLIS expression is encoded by chromosomal DNA.

Origin of nitrogen compounds in water infiltrating into porphyry quarry, Zalas, southern Poland.

The paper deals with the problem of the origin of nitric compounds in the mine water entering porphyry quarry. A hydrogeological study of the natural and disturbed ground water regimes as a consequence of quarrying have shown that fecal and domestic sewage was the main source of nitrogen compounds in the water entering the porphyry quarry. The sewage contaminated the surface water which in turn infiltrated into the porphyry within the cone of depression. In addition to that, NH 4 + and NO 3 − ions penetrated into the surface water as the result of washing of nitrogen out of the soil and a pa product of biochemical and chemical changes in the soil. A solution of unburnt explosives, used for the ground excavation was also a source of nitrogen compounds.

Nitrogen compounds in natural water—A review

Nitrogen compounds in natural water are significant in public health, agriculture, industry, and geochemistry. The many sources of nitrogen compounds and the deep involvement of nitrogen in the life processes of organisms makes the study of such compounds difficult. The sources include natural aerosols, precipitation, fixation by micro‐organisms in soil and water, decaying organic matter, and animal and industrial wastes, as well as probably undiscovered sources in consolidated and unconsolidated rocks. Nitrogen compounds are both oxidized and reduced by organisms. Some nitrogen compounds are adsorbed on clay. The theoretical end product in water and the compound probably most often determined is NO3−1. The concentration of nitregen compounds ranges from 0.0 to &gt;100 ppm (parts per million) in surface water and from 0.0 to &gt;1000 ppm in groundwater. Seasonal fluctuations occur. Much further research is needed, including improvements in methods of analysis, further investigation of sources, and detailed study of the nitrogen cycle in small drainage basins. (Key words: Geochemistry; quality of water.)

On the Nutrition of Ochromonas

ABSTRACT A species of the chrysophycean genus Ochromonas from England was grown in pure culture and compared with similar strains isolated in the United States. Although these organisms possess chromatophores with chlorophyll, they need organic substances not only as a source of nitrogen compounds, but as independent carbon and energy sources; they also require certain growth factors. Peptones are better as nitrogen sources than single amino-acids, few of which give satisfactory results. The utilization of inorganic nitrogen could not be demonstrated. As carbon sources sugars, alcohols, and fats, and, to a lesser degree, acetate, are suitable. Growth factors contained in liver extract, milk and yeast autolysate, and in smaller amounts in most peptones, are indispensable. Enzymes hydrolyzing starch, sucrose, fat, and protein are excreted. Ochromonas ingests and digests starch grains, casein, oil droplets, and small organisms. Neither bacteria nor yeast nor small algae were found able to support growth of any of the strains, even in the light. An additional carbon compound and possibly special vitamins are required. The hypothesis is put forward that phagotrophy chiefly serves for obtaining chemical compounds which the cell cannot synthesize itself and which have the character of vitamins. Phagotrophy therefore supplements the other means of nutrition, in the main qualitatively, while sugar, &amp;c., supplement it quantitatively and are indeed indispensable. The same media which support ample growth in the light do so also in the dark. This shows that photosynthesis is of little importance for the growth of the organisms, although it keeps them alive for long periods, while in the dark they soon die. Correlated with the manifold ways of acquiring food, there is also a remarkable variability in the size and shape of the cells, and still more in the dimensions and pigmentation of the chromatophores, and in the reserves stored as leucosin, volutin, and oil. These features which now prove to depend so much on conditions have previously been widely used in the diagnosis of species. The basis of classification should therefore be revised. Apart from the stigma, which is specific, use ought to be made only of those characters whose variation with conditions is reasonably well known. The extraordinary nutritional versatility of Ochromonas is considered to be a primitive character. From ancestors with a mixed nutritional habit, phototrophic, phagotrophic, and saprotrophic flagellates seem to have evolved, each with only one of the three modes of nutrition that are combined in Ochromonas.

Sources of Nitrogen Compounds

IN the Scientific American for April 21 Prof. T. H. Norton contributes a valuable article under the heading, “American Sources of Nitrogen”. Prof. Norton has given special attention to this important question, and the Department of Commerce published in 1912 an exhaustive report by him on “The Utilisation of Atmospheric Nitrogen.” In 1916 Congress appropriated the large sum of twenty million dollars for the purpose of constructing and organising Government works for the production of nitrogen compounds available for military requirements and for general economic purposes.

Sources of Nitrogen Compounds in the United States 1

THE problem of how to turn the vast store of uncombined nitrogen which exists in the atmosphere into useful products may be said to have been only seriously attacked within the lifetime of the present generation. It had its origin in the growing demand for forms of combined nitrogen suitable for use in the arts, and more particularly in agriculture, the oldest of all the arts. But circumstances arising out of the present world-wide struggle, affecting in greater or less degree every nation, but more particularly those engaged in the war, have forced the problem into still greater prominence by demonstrating how intimately it is bound up with the question of national defence. Indeed, as regards the Central Powers, their very existence is dependent upon it, as they now painfully realise.

Sources of nitrogen compounds in the United States

Sources of nitrogen compounds in the United States

On the Question whether Nitrites or Nitrates are produced by non-bacterial processes in the Soil

The experiments described in the following pages were made with a view to discover how far purely physical and chemical processes, known to take place in the soil, may be expected to give rise to nitrites and nitrates. Scattered throughout the extensive literature on nitrification are occasional papers tending to show, what might also be expected on theoretical grounds, that non-bacterial processes may be important sources of nitrates. Formerly these processes were considered to be the only sources, then came the brilliant researches of Schloesing and Muntz, Warington, Winogradsky, and others on nitrifying organisms, and non-bacterial processes were forced into the background. Now that a full knowledge of the various sources of nitrogen compounds in the soil has become so indispensable to the agriculturist, it seemed desirable to make a careful examination of the various chemical and physical processes known to take place in the soil, and to ascertain whether they make any direct or indirect contribution to its stores of nitrates. To this end we have repeated and extended such of the recorded observations bearing on the subject as seemed to merit repetition; we have also devised other experiments to make the examination as complete as possible.