Total concentrations of Ba, Cd, Co, Cr, Cu, Mn, Ni, Pb, V, Zn, and As together with Sr and Pb isotopic compositions of seabed sediments from the worldwide famous tourist destination of Acapulco Bay, Guerrero (southern Mexico) were determined to reveal the origin of detritus and toxic elements (TEs), their potential natural and anthropogenic sources, elemental distribution and their ecological risk. Sediments derive entirely from the nearby Acapulco Granite and their concentrations of TEs are variable and rather low, although, several are above the Local Geochemical Baseline in some sites of the bay. The enrichment factor (EF) and Pb isotopes indicate that TEs derive from the Acapulco Granite with contributions of an anthropogenic source represented, very likely, by ship-bottom paints. Wastewaters are a significant source of Pb and Cu. The ecological risk of TEs is low and only Cu represents a moderate ecological risk in a few sites.