Food Sources Research Articles

Deciphering Codon Usage Patterns in the Mitochondrial Genome of the Oryza Species

Rice (Oryza) is a genus in the Gramineae family, which has grown widely all over the world and is a staple food source for people’s survival. The genetic information of rice has garnered significant attention in recent years, prompting numerous researchers to conduct extensive investigations in this field. But rice mitochondrial codon usage patterns have received little attention. The present study systematically analyzed the codon usage patterns and sources of variance in the mitochondrial genome sequences of five rice species by the CodonW and R software programs. Our results revealed that the GC content of codons in rice mitochondrial genome genes was determined to be 43.60%. Notably, the individual codon positions exhibited distinct GC contents: 48.00% for position 1, 42.65% for position 2, and 40.16% for position 3. These findings suggest the preference of the rice mitochondrial genome for codons ending in A or U. A weak codon bias was observed, with the effective number of codons (ENC) varying between 40.02 and 61.00, with an average value of 54.34. Subsequently, we identified 25 identical high-frequency codons in five rice mitochondrial genomes, with 11 codons ending in A and 12 codons ending in U. The regression lines in the neutrality plot exhibited slopes of less than 0.5 in five rice species, indicating a predominant role of natural selection, while mutation pressure remained relatively insignificant. In the PR2-plot analysis, most of the genes were located in the right half of the plot, indicating that the third base of the synonymous codon was preferred to end in G than C. Additionally, the ENC plot and ENC ratio analysis unveiled that codon preferences in the rice mitochondrial genome were predominantly influenced by natural selection rather than mutational pressure. The analysis of correspondence revealed distinct variations in the codon usage pattern across five rice mitochondrial genomes. Based on the RSCU values of species, a cluster tree was inconsistent with the mitochondrial genetic data, indicating that RSCU data could not be used as a basis for classification at the species level in the Oryza genus. These results will help decide the specific types of natural selection pressures influencing codon usage and improve the expression of exogenous genes in rice mitochondrial genomes by optimizing their codons.

Carotenoid composition and sequestration in cassava (Manihot esculentum Crantz) roots

Cassava (Manihot esculentum Crantz) is a staple food source for many developing countries. Its edible roots are high in starch but lack micronutrients such as β-carotene. In the present study, analysis of pedigree breeding populations has led to the identification of cassava accessions with enhanced β-carotene contents up to 40 μg/g DW. This represents 0.2% of the Recommended Daily Allowance (RDA) for vitamin A. The β-branch of the carotenoid pathway predominates in cassava roots, with dominant levels of β-carotene followed by other minor epoxides of β-ring derived carotenoids. Metabolomic analysis revealed that steady state levels of intermediary metabolism were not altered by the formation of carotenoids, similar to starch and carbohydrate levels. Apocarotenoids appeared to be independent of their carotenoid precursors. Lipidomic analysis provided evidence of a significant positive correlation between carotenoid and lipid content, in particular plastid specific galactolipids. Proteomic analysis of isolated amyloplasts identified the majority of proteins associated with translation and carbohydrate/starch biosynthesis (e.g. glucose-1-phosphate adenylyltransferase). No carotenoid related proteins were detected even in the highest carotenoid containing lines. Carotenoids were associated with fractions typically annotated as plastoglobuli and plastid membranes (particularly the envelope). Proteomic analysis confirmed these structures apart from plastoglobuli, thus potentially amyloplast structures may not contain classical plastoglobuli structures.

Chlorella vulgaris as a food substitute: Applications and benefits in the food industry

AbstractChlorella vulgaris, a freshwater microalga, is gaining attention for its potential as a nutritious food source and dietary supplement. This review aims to provide a comprehensive discussion on C. vulgaris, evaluating its viability as a food substitute in the industry by exploring the nutritional value and application of C. vulgaris in the food industry. Rich in protein, lipids, carbohydrates, vitamins, and minerals, Chlorella offers substantial nutritional benefits, positioning it as a valuable food substitute. Its applications in the food industry include incorporation into smoothies, snacks, and supplements, enhancing the nutritional profile of various food products. The health benefits of Chlorella encompass antioxidant activity, immune system support, and detoxification, contributing to overall well‐being. Despite these advantages, the commercialization of Chlorella faces significant challenges. These include variability in antibacterial activity due to strain and growth conditions, high production costs, contamination risks, and sensory issues such as unpleasant taste and smell. Additionally, Chlorella can accumulate heavy metals from its environment, necessitating stringent quality control measures. Future prospects involve improving Chlorella strains through genetic manipulation to enhance nutrient content, developing cost‐effective culture systems, and exploring advanced processing techniques like pulsed electric fields for better digestibility. Addressing sensory issues through flavor‐masking strategies and employing environmental management practices will further support Chlorella's integration into the food industry. Although C. vulgaris shows great potential as a nutritious food ingredient, overcoming existing challenges and optimizing production methods would be crucial for its successful adoption and widespread use.

SOSIALISASI DAN PEMANFAATAN LIMBAH DAPUR (SAYURAN) UNTUK PEMBUATAN PUPUK ORGANIK CAIR DI DESA NUANEA KECAMATAN AMAHAI KABUPATEN MALUKU TENGAH

This research was conducted in contry Nua Nea, Amahai subdistrict, Central Maluku Regency, to over come the problem of lack of fertilizer subsidies and high fertilizer prices. The aim is to provide solutions to farmers through socialization and production of liquid organic fertilizer (POC). The research involves KKN activies that involve students in assisting the community. The material presented includes the benefits of kitchen waste as a source of nutrients and methods for making POC. The research results show that this activity provides new knowledge to the community and incraises awareness of environmental cleanlinees the agricultural potential and environmental cleanliness of Nua Nea state.

Impacts of Supplemental Feeding on Sunbird-Pollination Systems in Young Fynbos Varies with Floral Abundance.

Supplementary sugar-water feeding offers nectarivorous birds a source of spatially concentrated food, as opposed to the dispersed food available in flowers. This could impact bird visits to native flowers and alter plant-bird mutualisms, particularly in young post-fire vegetation. This study examined the effects of sugar-water feeders on nectarivorous birds and their plant mutualists in young (burned 18 months previously) and transitional vegetation (burned 5 years previously). A supplementary feeding experiment was conducted at Grootbos Private Nature Reserve in South Africa, using sugar-water feeders when floral abundance was low (winter) and high (spring). We compared bird abundance and visitation rates to flowers before, during, and after feeder presence in both seasons. The use of sugar-water feeders by nectarivorous birds was inversely related to floral abundance, with 679 bird visits (6.94 ± 1.40 bird visitation rate per hour) to feeders in winter and only 90 visits (0.41 ± 0.16 visitation rate per hour) during spring. Bird visits were higher at flowers than at sugar-water feeders, in both seasons. Sugar-water feeders did not influence the visitation rate of sunbirds to flowers in both seasons, contrasting with findings from areas abutting suburbia, suggesting that feeder influence on bird visitation rate may not be apparent in areas with no history of sugar-water feeders. We find that low numbers of feeders do not necessarily compete with natural nectar resources but may instead provide birds with an additional food source, particularly when floral resources are low.

Metal-phenolic nanoparticles enhance low temperature photothermal therapy for bacterial biofilm in superficial infections.

Bacterial infections, especially induced by multidrug-resistant pathogens, have become a significant global health concern. In the infected tissues, biofilms not only serve as a source of nutrients but also act as protective barriers that impede antibiotic penetration. Herein, we developed tea polyphenols epigallocatechin gallate (EGCG) Au nanoparticles (E-Au NPs) through direct one-step self-assembly methods by EGCG chelating with Au ions to eradicate antibiotic-resistant bacteria methicillin-resistant Staphylococcus aureus (MRSA) and prevent the formation of biofilm under near-infrared (NIR) irradiation. The outstanding antibacterial effect involved in mild photothermal therapy, reactive oxygen species production, pathogenicity-related genes regulation, and quinoprotein formation that were specific to the polyphenol-based NPs. The excellent antibacterial and anti-inflammatory therapeutic efficacy of E-Au NPs was validated and topically applied in murine MRSA-infected skin wounds and keratitis model in vivo to kill bacteria, reduce the inflammation response and promote wound healing. Furthermore, the ophthalmic and systemic biosafety profiles were thoroughly evaluated while no significant side effects were revealed achieving a balance between high-efficiency antibacterial properties and biocompatibility. This study provides an effective therapeutic agent of metal-phenolic materials for superficial tissue infection with favorable prognosis and potential in clinical translation.

EKSPLORASI KEANEKARAGAMAN BURUNG SEBAGAI DAYA TARIK UTAMA AVITOURISM DI EKOWISATA MANGROVE CUKU NYINYI, KABUPATEN PESAWARAN

Birds play a significant ecological role and are also attractive for birdwatching tourism (avitourism), particularly in mangrove ecosystems which provide habitat and food sources. Exploring the avian diversity in the Cuku NyiNyi Mangrove Ecotourism Area for avitourism development can be a conservation effort for birds and provide economic incentives for local communities. This study aimed to identify bird species as potential attractions for avitourism in the Cuku NyiNyi Mangrove Ecotourism Area. Data analysis involved determining the conservation status and criteria of birds as potential objects and attractions for avitourism. A total of 32 bird species from 19 families were identified with a total of 113 individuals. Several bird species have conservation statuses that require attention, namely the cinnamon-headed green pigeon (Treron fulvicollis) which is categorized as vulnerable (VU) and the brahminy kite (Haliastur indus), crested serpent eagle (Spilornis cheela), racket-tailed treepie (Crypsirina temia), and malaysian pied fantail (Rhipidura javanica) which are protected species. The analysis showed that 18 bird species have the potential to be objects and attractions for avitourism based on criteria such as raptors, protected species, songbirds, endemic birds, and colorful birds. The avian diversity in the Cuku NyiNyi Mangrove Ecotourism Area supports the potential for avitourism to develop as a form of sustainable tourism for the protection of bird species while attracting tourists, especially birdwatchers.

Climate Change Affects Multiple Coral Reef Fisheries Ecosystem Services

ABSTRACTCoral reef fisheries support livelihoods and provide an affordable source of essential dietary nutrients to coastal people. However, climate‐driven coral bleaching is dramatically altering reef habitats and changing reef fish composition, diversity, and productivity. We used data from fisheries independent surveys and artisanal trap landings to explore how reefs with different responses to coral bleaching differed in habitat configurations, fisheries catch rates, economic returns, and nutritional content. Coral‐associated fishes supported the highest catch rates, greatest species diversity, highest rates of economic returns for fishers, and a wide price range for consumers. Macroalgae‐associated fishes supported the greatest overall fisheries yields, which indicated higher fishery dependence on these habitats. Nutrient content was high in catches across all habitats, but concentrations varied by nutrient, which suggested that fishing across a range of reef habitats should provide the greatest variety of nutrients to coastal communities.

Phytochemical screening, nutritional, antinutritional, antioxidant, GCMS and mineral analysis of Zanthoxylum rhetsa (Roxb.) DC: Insights from tribal consumption in Mizoram, Northeast India

The plant Zanthoxylum rhetsa Roxb, a member of the Rutaceae family, holds dual significance in certain communities, particularly in Mizoram, Northeast India, where it is valued for its medicinal properties and consumed as a vegetable. This study comprehensively analyzes the phytochemical, nutritional, antinutritional, and antioxidant properties and the mineral content of Z. rhetsa, a plant traditionally consumed by tribal communities in this region. Minerals such as aluminum, copper, iron, manganese, nickel, and zinc were measured using Atomic Absorption Spectroscopy (AAS). Gas Chromatography-Mass Spectrometry (GC-MS) analysis was conducted to determine the chemical composition of the samples. Phytochemical screening revealed the existence of several bioactive components, such as tannins and flavonoids, while nutritional studies validated the plant's abundance of key nutrients. Among the minerals, manganese was found in the highest concentration (481.356 mg/100 g of DW), while nickel was present in the lowest concentration (1.614 mg/100 g of DW). The total calorific content was ascertained to be 51.344 Kcal/100g, adequate for dietary requirements. Additionally, the total phenolic content measured was 8.28 mg/g in gallic acid equivalent, and the total flavonoid content was 43.04 mg/g in quercetin equivalents. The existence of antinutritional compounds in differing amounts necessitates meticulous evaluation, particularly with the intake of fruits that may possess elevated levels of these substances. This comprehensive assessment highlights the nutritional significance of Z. rhetsa in local diets, emphasizing its dual role as a source of beneficial nutrients and potential antinutritional factors.

Khajur (Phoenix dactylifera L.): Medicinal importance in perspective of Unani medicine and pharmacological studies

The date palm (Phoenix dactylifera L.) is recognized as one of humanity's earliest cultivated plants, having served as a food source for over 6000 years. It holds a prominent status as the most favoured fruit in the Middle East and North Africa. Date fruit has a long history of consumption and traditional medicinal use. Whether eaten fresh or dried, dates offer a rich source of energy due to their exceptional nutritional content and extended shelf life. They provide an economical means of obtaining essential nutrients including carbohydrates, proteins, vitamins, minerals and dietary fibers. Furthermore, date fruit exhibits a diverse range of pharmacological effects including antimicrobial, anti-inflammatory, anti-diabetic, anticancer, antihyperlipidemic and many more. It is also beneficial in disorders of paralysis, sexual debility, fever etc. Date palm fruits offer a natural and safe alternative or complement to synthetic drugs in managing numerous health conditions. Keywords: Dates, Phoenix dactylifera, Arecaceae/Palmae, Khajur, Unani Medicine.

Morphological and Productivity Response of Chickpea (Cicer arietinum L.) to Nano Biofertilization

Background: Chickpea productivity in the last decade has been declined due to improper management of inorganic and organic nutrient sources and it has been contended that the usual native soil rhizobial populations are insufficient/ineffective in N2 – fixation. Rhizobium based biofertilizers may substitute nitrogenous chemical fertilizers and provide a suitable way of achieving sustainable production. The effective use of exogeneous application of nano emulsion biofertilizer can enhance yield by reducing flower drop, regulating pod formation besides improving soil quality. In view of above consideration, the present investigation entitled “Morphological and productivity response of chickpea (Cicer arietinum L.) to nano biofertilization” was carried out. Methods: The field experiment was conducted on sandy clayey loam soil during two consecutive Rabi seasons of 2022-2023 and 2023-2024. The experiment was laid out in randomized complete block design (Factor) with three replications, assigning 45 treatments consisting of five soil applications (100% RDF; 100% RDF + FYM; 100% RDF + Vermicompost; 75% RDF + FYM; and 75% RDF + Vermicompost) as first factor and three foliar applications (Nano DAP; Nano Urea and Nano emulsion biofertilizer) as second factor treatments. Result: Application of Nano emulsion biofertilizer to chickpea variety (NBeG-49) at harvest had shown significant higher plant height (35.03 cm), dry matter production (3251.43 kg ha-1), leaf area index (0.90), crop growth rate (1.59 gm-2 day-1), relative growth rate (26.3 mg g-1 day-1), chlorophyll (SPAD value) (58.22), number of pods/plant (24.93), seed yield (1111.60 kg ha-1), straw yield (2501.10 kg ha-1) and nitrogen uptake (70.56 kg ha-1), phosphorus uptake (203.21 kg ha-1) and potassium uptake (54.30 kg ha-1).

Globalization and Conflicts: the Good, the Bad, and the Ugly of Corporations in Africa

Abstract Using a novel georeferenced datataset on the affiliates and headquarters of multinational enterprises between 2007 and 2018 together with georeferenced conflict data for the African continent, this work establishes a causal link between the activities of multinational enterprises and violent conflicts: multinationals’ activity increases the number of conflicts. This applies particularly to sectors intense in scarce resources, especially land. As farming is the primary source of food and income for Africans, land-intensive activity on the part of the multinationals increases local grievance, escalating to violent actions. These effects are magnified in areas targeted for large-scale land acquisitions.

Evaluating the impact of Cold plasma on Seedling Growth properties, seed germination, and soybean antioxidant enzyme activity.

Cold atmospheric pressure plasma (CAP) has garnered significant attention in recent years for its potential applications in biomedical, environmental, and agricultural fields. Cold plasma treatment exhibits a variety of effects in agricultural applications, including impacts on seed germination and seedling growth; however, further research is required. Soybean serves as a fundamental source of nutrients for both animals and humans. Soybean seeds possess impermeable and thick testae, which results in prolonged germination times and suboptimal germination rates. The soybeans exhibit low uniformity. As a result, poor crop establishment and yield reduction are inevitable outcomes. Therefore, the purpose of this study was to examine the effects of Iranian soybean cultivars, such as Sari, Saba, Arian, Katoul, and Williams, on seedling growth properties, seed germination, and antioxidant enzyme activity, using argon at time intervals of 30, 60, 180, 300, and 420s. Cold plasma treatment significantly enhanced germination potential from 1.18 to 66.97%, germination index from 0.50 to 60.09%, germination rate from 1.78 to 32.17%, seedling length from 2.70cm to 78.13cm, root length from 2.87cm to 56.13cm, and seedling dry weight from 1.80g to 36.63g. Additionally, CAT activity increased from 0.88- to 4.40-fold, SOD activity from 0.86- to 5.89-fold, and APX activities from 0.40- to 4.01-fold compared to the control treatment. The findings indicated that the samples exhibited optimal results at treatment durations of 60 and 180s. The influence of plasma on the antioxidant responses of seedlings, seed germination, and growth characteristics was contingent upon the duration of treatment. Cold plasma, when applied for an appropriate duration, may enhance soybean seedling growth characteristics and seed germination.

Microalgae: a multifaceted catalyst for sustainable solutions in renewable energy, food security, and environmental management.

This review comprehensively examines the various applications of microalgae, focusing on their significant potential in producing biodiesel and hydrogen, serving as sustainable food sources, and their efficacy in treating both municipal and food-related wastewater. While previous studies have mainly focused on specific applications of microalgae, such as biofuel production or wastewater treatment, this review covers these applications comprehensively. It examines the potential for microalgae to be applied in various industrial sectors such as energy, food security, and environmental management. By bridging these different application areas, this review differs from previous studies in providing an integrated and multifaceted view of the industrial applications of microalgae. Since it is essential to increase the productivity of the process to utilize microalgae for various industrial applications, research trends in different microalgae cultivation processes, including the culture system (e.g., open ponds, closed ponds) or environmental conditions (e.g., pH, temperature, light intensity) to improve the productivity of biomass and valuable substances was firstly analyzed. In addition, microalgae cultivation technologies that can maximize the biomass and valuable substances productivity while limiting the potential for contamination that can occur when utilizing these systems have been described to maximize CO2 reduction. In conclusion, this review has provided a detailed analysis of current research findings and technological innovations, highlighting the important role of microalgae in addressing global challenges related to energy, food supply, and waste management. It has also provided valuable insights into future research directions and potential commercial applications in several bio-related industries, and illustrated how important continued exploration and development in this area is to realize the full potential of microalgae.

Transcriptome profiling uncovers differentially expressed genes linked to nutritional quality in vegetable soybean.

Vegetative soybean (maodou or edamame) serves as a nutrient-rich food source with significant potential for mitigating global nutritional deficiencies. This study undertook a thorough examination of the nutritional profiles and transcriptomic landscapes of six soybean cultivars, including three common cultivars (Heinong551, Heinong562, and Heinong63) and three fresh maodou cultivars (Heinong527, HeinongXS4, and HeinongXS5). Nutrient analysis of the seeds disclosed notable differences in the levels of protein, fat, soluble sugars, vitamin E, calcium, potassium, magnesium, manganese, iron, and zinc across the cultivars. Through comparative transcriptome profiling and RNA sequencing, distinct variations in differentially expressed genes (DEGs) were identified between fresh and traditional maodou cultivars. Functional enrichment analyses underscored the involvement of DEGs in critical biological processes, such as nutrient biosynthesis, seed development, and stress responses. Additionally, association studies demonstrated robust correlations between specific DEG expression patterns and seed nutrient compositions across the different cultivars. Sankey diagrams illustrated that DEGs are strongly linked with seed quality traits, revealing potential molecular determinants that govern variations in nutritional content. The identified DEGs and their relationships with nutritional profiles offer valuable insights for breeding programs focused on developing cultivars with improved nutritional quality, tailored to specific dietary needs or industrial applications.

Determination of Vitamin C Concentration in Various Fresh Orange and Lemon Samples from Janzour Region Using Volumetric Titration

Vitamin C is an essential nutrient crucial for human health, playing a vital role in numerous physiological processes, including immune system support and collagen synthesis. Citrus fruits, such as oranges and lemons, are recognized as rich sources of this vitamin. The primary purpose of this study is to determine the vitamin C concentration in various fresh orange and lemon juices. The samples were randomly collected from the local market in Janzour. The study included 18 samples representing 6 different citrus varieties (3 samples per variety). Vitamin C concentration was statistically estimated using a titrimetric method with a standardized solution of 2,6-dichlorophenolindophenol (DCPIP) as the titrant. The obtained results revealed some kind of variations in vitamin C content among different citrus varieties and even within samples of the same variety. Abu Surah orange samples exhibited the highest vitamin C concentration (mean range: 38.9-41.6 mg/dl), while sweet orange samples had the lowest (mean range: 5.1- 8.5 mg/dl). Vitamin C concentrations in other varieties ranged as follows: Sour orange: 9.22-24.9 mg/dl, Mandarin: 9.0 -16.2 mg/dl, Tarocco: 28.2-33.3 mg/dl, and Lemon: 14.5-15.8 mg/dl. These results showed evidently that vitamin C levels were varied even within the same sample type. Such variations would draw attention to the importance of choosing varieties when searching for food sources of this vital nutrient.

Quality assessment of yellow mealworm (Tenebrio molitor L.) powders processed by pulsed electric field and convective drying.

Edible insects offer opportunities for food production, as they are an interesting source of many nutrients. In this study, the effect of pulsed electric field (PEF) and convective drying on the chemical composition with emphasizing the fat properties as well as physical, techno-functional, and thermal properties of yellow mealworm powders was investigated. The chemical composition of the yellow mealworm powders differed by PEF. When PEF was applied at 20 and 40kJ/kg, the moisture, ash, and protein content were significantly lower, while the fat extraction yield significantly increased compared to the control sample. Furthermore, the fat extracted from these samples was characterized by a higher proportion of saturated and monounsaturated fatty acids as well as a higher thrombogenicity index, which is not beneficial from a nutritional point of view. After treatment with PEF at 5kJ/kg, the powder was the lightest, redness and yellowness. Moreover, the highest hygroscopicity, water activity, and water and oil binding capacity for this powder were determined. The results revealed that yellow mealworm powders are a good source of macronutrients and exhibit beneficial techno-functional properties, nevertheless, the drawback is their high cohesiveness (1.27-1.44), which can be difficult to apply under industrial conditions.

Chemical Quality Parameters of Mandarin (Citrus reticulata Blanco.) Fruits Influenced by different Organic Sources

A field experiment was carried out during the year 2021-22 and 2022-23 on 12 years old mandarin plants at the Instructional Farm, Department of Fruit Science, College of Horticulture and Forestry, Jhalawar. The experiment was consisting of 21 treatments of different organic source of NPK viz., vermicompost, cotton fortified vermicompost, neem cake, cotton cake, mustard cake and bio fertilizers such as PSB and VAM with three levels of recommendation dose of fertilizers. The experiment was laid out in randomized block design with three replications. Among different treatments, treatment T9 (75 % RDF + 10 kg Vermicompost + 7.5 kg Neem cake + 50 g PSB) was found best with regards to maximum pooled TSS (11.74 0B), reducing sugar (6.30%) non-reducing sugar (2.62%), total sugar (8.92%) per cent, sugar/acid ratio (14.40), ascorbic acid (53.08mg/100g) and juice (46.57%). Therefore, based on two years experimentation, use of organic source of nutrients with bio-fertilizers favoured biochemical compounds of Nagpur Mandarin fruits.

Contrasting copepod community composition in two Greenland fjords with different glacier types

Abstract Greenland's fjord ecosystems are strongly influenced by meltwater discharge from glaciers. Marine-terminating glaciers can significantly enhance primary production during the melt season (compared to land-terminating glaciers), but their impact on secondary producers is not well understood. Here, we study seasonal changes in the zooplankton community (>50 μm) and grazing rates along two fjord transects in southwest Greenland influenced by different glacier types. Zooplankton biomass was comparable between the fjords, but community composition differed during summer. Nuup Kangerlua, a predominantly marine-terminating system, was characterized by large, herbivorous copepods and a longer summer grazing period in connection with the summer bloom. Ameralik, influenced by a land-terminating glacier, was characterized by small, omnivorous copepods, particularly Microsetella norvegica and Oncaea spp., and indicated a faster post-bloom transition towards regenerated production. Local hydrographic conditions also impact copepod biomass. A station with high biomass was found in Ameralik, potentially linked to a frontal zone where fjord water masses meet, providing favorable grazing conditions. We hypothesize that a future transformation from marine- to land-terminating glaciers could result in an increased abundance of smaller copepods, as observed in Ameralik. Such a community would constitute a less lipid-rich food source for higher trophic levels.

Food and Labour under Imperial Rule: Unravelling the Food Landscape of Transplanted Workers (mitmaqkuna) in the Inka Empire

The Inka empire's expansion incorporated diverse cultural and ecological elements in microcosmic representations of their empire. Imperial practices included the resettlement of communities from various regions into labour enclaves near Inka ceremonial, administrative and economic hubs. This degree of imperial control might suggest a limitation on Inka subjects’ freedom to integrate non-local food resources into their diets. Employing starch grain analysis from stone tools, we seek to identify the range of plant food sources and examine the extent to which the Inka imposed constraints on inter-community interactions and the exchange of comestibles. Focusing on a translocated labour force residing near the Inka provincial centre of Vilcashuamán, our findings reveal the consumption of a variety of edible plants originating from multiple, occasionally distant, ecological regions. The results indicate that, in contrast to the restrictions on trade of other commodities as recorded in ethnohistorical accounts and previous archaeological research, the exchange of edible plant species among the subjugated peoples may have been less regulated. This study demonstrates how food landscapes potentially served as loci of resistance to the Inka empire's manipulative cosmopolitanism.