The mussel Mytilus galloprovincialis is a bivalve mollusc of high commercial interest, which frequently breeds in coastal areas with high levels of ship traffic noise. Studies on the effect of this stressor on mussels are scarce, which is why we set out to design a system to study the effect of ship noise on mussel behaviour (valve opening-closure rhythms). The proposed experimental set-up uses a high frequency non-invasive valvometry system to detect shell closure reactions related to instantaneous increases in sound level. The main findings are that mussels reacted to ship noise levels above 114 dB re 1μPa (63Hz-4kHz), that most reactions occurred within 13 s after a sudden increase in level, and that there does not appear to be a clear relationship between closure depth and noise level. Although mussels may not be sensitive to sound pressure but only to the associated particle movement, the results of this work contribute to highlighting the sensitivity of this species to noise, and the need to carry out experiments with a larger number of specimens and in conditions that allow the acoustic field to be reproduced more realistically.