Sorghum Meal Research Articles

Effect of Fermentation and/or Cooking on Some Nutritional Quality of Sorghum Meals Formulated with Different Ratios of Wheat and Pumpkin Flour

Sorghum cultivar (Tabat), defatted pumpkin seed pulp (DPSP), and wheat flour were used to prepare four meals to study the effects of component ratio and fermentation and/or cooking on various nutritional qualities. Before and after processing, the meals' chemical compositions, total calories, minerals, antinutrients, and in vitro protein digestibility (IVPD) were assessed. The addition of wheat and pumpkin flour resulted in a decrease in carbohydrates and antinutrients and an increase in ash, protein, fiber, fat, total and extractable minerals, and IVPD. After fermentation, the antinutrients were significantly (P ≤ 0.05) reduced, resulting in meals of excellent quality. Cooking the fermented dough afterward, nevertheless, revealed much greater improvement. The proportion of ingredients decreased the effect of cooking on chemical composition and led to a substantial (P ≤ 0.05) reduction in tannin and phytate, which was accompanied by a significant (P ≤ 0.05) increase in IVPD and mineral extractability. Meals were accepted more often than the control group (P ≤ 0.05). Out of all the formulations, the one with 10% wheat and 20% DPSP flours (M3) was found to be the most nutrient-dense and bioavailable.

The promoting effect of electron beam irradiation on enzymatic saccharification and alcohol fermentation of sorghum meal: Related mechanisms

In this study, sorghum meal underwent 2–10 kGy electron beam irradiation (EBI) to investigate alterations in its elemental composition, relevant physicochemical characteristics, and enzymatic hydrolysis fermentation properties. The results indicated a decrease in amylose content and an increase in reducing sugar content in sorghum meal after EBI, along with severe damage to the internal structure of starch granules, leading to increased solubility in water. Furthermore, irradiation enhanced the shear resistance and gelatinization stability of sorghum meal paste. Most importantly, EBI-induced degradation of sorghum starch molecules enhanced their susceptibility to interact with α-amylase, significantly improving the enzymatic hydrolysis and alcohol fermentation efficiency of sorghum meal. At an irradiation dose of 8 kGy, raw and cooked sorghum meal exhibited a 43.40% and 33.33% increase in ethanol concentration and a 43.44% and 30.29% increase in ethanol yield, respectively. This finding indicated that irradiation is an effective pretreatment method for starch-based ethanol fermentation.

Aflatoxins contents determination in some foodstuffs in Burkina Faso and human health risk assessment.

Aflatoxins are produced by fungi of the genus Aspergillus that colonize many foodstuffs during agricultural production, harvesting, transportation, storage, and food processing. In view of these aflatoxins toxicity to humans, their presence in foods such as cereals and oilseeds constitutes a major challenge for global food security, health and nutrition. This study was therefore initiated to assess the level of aflatoxin contamination of various foodstuffs sold in urban and semi-urban markets in Burkina Faso, and to evaluate the carcinogenic risk which the consuming population is exposed to. Two hundred and twelve foodstuff samples were collected in two large cities (Ouagadougou and Bobo Dioulasso) and three semi urban localities (Cinkansé, Dakola and Niangoloko). Aflatoxins contents in foodstuffs were determined by immunoaffinity chromatography and human health risk assessment was performed by using the Monte Carlo algorithm. The aflatoxins contents determination showed that 41.50% of studied samples were contaminated with concentrations up to 182.28 μg/kg for AFB1 in peanuts. Chronic Daily Intake, calculated based on the consumption patterns assumed in this study, was estimated to be higher in large cities (CDI = 33.68 μg/kg bw in Ouagadougou and 10.18 μg/kg bw in Bobo Dioulasso) than in semi urban localities (CDI = 4.29 μg/kg bw in Cinkansé, CDI = 0.39 μg/kg bw in Dakola and CDI = 0.18 μg/kg bw in Niangoloko). The MOE determination showed that the sorghum meal and whole grain maize consumption was associated to the carcinogenic risk for public health in large cities (the percentile 95 of MOE = 3316 for rice, 4511 for peanuts, 3334 for sorghum meal and 4530 for whole grain maize). In semi urban localities, no carcinogenic risk was observed to public health. These results should inspire the country's sanitary and agricultural authorities to undertake actions to fight against the agricultural food products contamination by aflatoxins in order to safeguard the population's health.

Partial replacement of maize meal with high-tannin sorghum meal affects finishing and methane emissions of Pedi goats

This study was conducted to determine the effect of replacing maize meal with Sorghum vulgare meal, a high-tannin sorghum meal, in fattening diets on bodyweight and methane emissions by yearling male Pedi goats. A total of 12 yearling male Pedi goats with an average initial liveweight of 14 ± 1 kg were randomly assigned to a complete randomized design with four treatments, which were formulated such that 0%, 10%, 20% and 30% of the maize meal was replaced with Sorghum vulgare meal. The experiment was conducted over 28 days, with 21 days adaptation, followed by seven days for sample collection. Replacing maize meal with sorghum did not produce detectable (P >0.05) effects on bodyweight and live weight gain. The various replacement levels of sorghum meal had no detectable effects on the blood cell profile of Pedi goats either. However, they reduced methane emission and improved feed conversion ratio (P <0.05), which allowed for an approximation of the feed required by growing goats. These findings may assist farmers in selecting and utilizing the right feeds to maximize profitability in the small-stock farming sector.

Effect of Cereal α-Amylase/Trypsin Inhibitors on Developmental Characteristics and Abundance of Digestive Enzymes of Mealworm Larvae (Tenebrio molitor L.).

Simple SummaryThe main nutritionally relevant proteins of Tenebrio molitor L. larvae are cereal proteins. Cereals contain α-amylase/trypsin inhibitors (ATIs) that interact with digestive enzymes and which may impair the growth of the larvae. Despite the existing work on the subject, there is still a lack of information regarding the effects of ATIs on the relative abundance of various enzymes in larvae. Our aim was therefore to undertake an assessment of the potential effects of ATIs on the growth parameters and digestive enzyme contents of T. molitor. The larvae were fed with cereal meals containing different levels of ATIs. The developmental characteristics were evaluated and finally an analytical method based on liquid chromatography with tandem mass spectrometry (LC-MS/MS) was developed to quantify the relative abundance of enzymes in the larvae. The results indicated an increase in pupation and significantly higher protein concentrations in larvae fed with wheat meals compared to those fed with sorghum meals. Patterns of specific α-amylase activity (in mM maltose/min/mg protein) were similar to those of amylase activity (in mM maltose/min) and the larvae fed on high-ATI-content meals exhibited an increased death rate, although the results were not always significantly consistent. The results of the LC-MS/MS analysis showed a decrease of about half of the relative content of α-amylase among the three proteases monitored, as well as an increase in dipeptidylpeptidase I and chymotrypsin, whereas trypsin remained constant. Therefore, these results indicate that meal composition has an effect on the expression of T. molitor digestive enzymes.The objective of this work was to investigate the potential effect of cereal α-amylase/trypsin inhibitors (ATIs) on growth parameters and selective digestive enzymes of Tenebrio molitor L. larvae. The approach consisted of feeding the larvae with wheat, sorghum and rice meals containing different levels and composition of α-amylase/trypsin inhibitors. The developmental and biochemical characteristics of the larvae were assessed over feeding periods of 5 h, 5 days and 10 days, and the relative abundance of α-amylase and selected proteases in larvae were determined using liquid chromatography tandem mass spectrometry. Overall, weight gains ranged from 21% to 42% after five days of feeding. The larval death rate significantly increased in all groups after 10 days of feeding (p < 0.05), whereas the pupation rate was about 25% among larvae fed with rice (Oryza sativa L.) and Siyazan/Esperya wheat meals, and only 8% and 14% among those fed with Damougari and S35 sorghum meals. As determined using the Lowry method, the protein contents of the sodium phosphate extracts ranged from 7.80 ± 0.09 to 9.42 ± 0.19 mg/mL and those of the ammonium bicarbonate/urea reached 19.78 ± 0.16 to 37.47 ± 1.38 mg/mL. The total protein contents of the larvae according to the Kjeldahl method ranged from 44.0 and 49.9 g/100 g. The relative abundance of α-amylase, CLIP domain-containing serine protease, modular serine protease zymogen and C1 family cathepsin significantly decreased in the larvae, whereas dipeptidylpeptidase I and chymotrypsin increased within the first hours after feeding (p < 0.05). Trypsin content was found to be constant independently of time or feed material. Finally, based on the results we obtained, it was difficult to substantively draw conclusions on the likely effects of meal ATI composition on larval developmental characteristics, but their effects on the digestive enzyme expression remain relevant.

Effects of complete replacement of corn flour with sorghum flour in dairy cows fed Parmigiano Reggiano dry hay-based ration

The aim of this research was to evaluate of the productive responses of cows fed a dry hay based total mixed ration (TMR) in which sorghum (SOR) or corn (COR) meal (8 kg/h/d) were the main source of starch. The study involved two dairy herds located in the Parmigiano Reggiano PDO area, for a total of 1,400 cows (30% primiparous and 70% pluriparous). Each herd was fed alternatively SOR or COR TMR for 4 periods of 3 weeks: two weeks of adaptation and one week of data collection. Total milk production and composition, cheesemaking properties, fatty acid content and cheese yield were measured. In addition, within each herd, a random subgroup of 50 cows was selected for individual milk production, composition and cheesemaking properties analysis. Fibre digestibility was evaluated on faecal samples collected in 15 cows randomly selected in each subgroup. Data were analysed by a linear mixed model procedure with diet, herd, days in milk, parity and their interactions as fixed effects and cow as random effect. Individual milk production increase in SOR (32.43 vs. 31.34 kg, for SOR and COR, respectively; p<.0001) however, bulk milk and cheese yield did not show differences. Milk urea content was higher in SOR (27.38 vs. 22.79 mg/dL, for SOR and COR, respectively; p < .05). In this study the complete replacement of corn with finely ground sorghum meal in dairy cow diets in the Parmigiano Reggiano region did not result in negative effects on cows’ productivity, cheese making properties and production. Highlights Sorghum meal can be used as a substitute of corn without negative effect on herd productivity, milk quality and cheese yield. Replacement of corn meal with sorghum meal in the dairy cows’ ration permit to decrease the soy content of the ration, thus improving sustainability and costs of feeding. Utilising sorghum meal could increase the amount of crop produced in the geographical area of the farm, contributing to increase its economic sustainability.

Sorghum-Insect Composites for Healthier Cookies: Nutritional, Functional, and Technological Evaluation.

Protein-energy malnutrition (PEM) is a major health concern in sub-Saharan Africa (SSA). Relying on unexploited and regionally available rich sources of proteins such as insects and sorghum might contribute towards addressing PEM among at-risk populations. Insects are high in nutrients, especially protein, and are abundant in SSA. Sorghum is adapted to the tropical areas of SSA and as such it is an appropriate source of energy compared with temperate cereals like wheat. It is necessary to assess whether cookies fortified with sorghum and termite would be suitable for use in addressing PEM in SSA. Whole grain sorghum meal and termite meal were mixed at a 3:1 ratio (w/w sorghum:termite) to form a sorghum–termite meal blend. Composite cookies were prepared where the sorghum–termite blend partially substituted wheat flour at 20%, 40%, and 60% (sorghum–termite blend:wheat flour (w/w). The functional and nutritional qualities of the cookies were assessed. Compared with the control (100% wheat flour), the cookies fortified with sorghum and termite had about double the quantity of protein, minerals, and amino acids. However, with increased substitution level of the sorghum–termite blend, the spread factor of the cookies decreased. There is a potential to incorporate sorghum and termite in cookies for increased intake of several nutrients by communities that are vulnerable to nutrient deficiencies, especially PEM.

Effect of low tannin sorghum based feeds on broiler meat nutritional quality

This study determined the meat quality characteristics of different parts of a broiler carcass obtained from chicken fed on diets formulated using either maize ( Zea mays ) or sorghum ( Sorghum bicolor ) as the source of energy. Six diets that differed on the sorghum/maize replacement levels (0%, 20%, 40%, 60%, 80% and 100%) were fed to 120-day old broiler. The chicken meat was harvested after 8 weeks and meat from different parts of the carcasses were analysed for fatty acid profile, cholesterol, vitamins A and E content. Levels of 36.37%–80.32% of saturated fatty acids were detected in different cuts. The polyunsaturated fatty acids ranged at 12.36%–29.13% while the monounsaturated fatty acids accounted for 1.46%–4.84% for all the experimental diets. The highest levels of long chain unsaturated fatty acids (18.68%–22.87%) were detected in the thigh muscle meat of chicken fed on a 0% sorghum diet. Conversely, diet that had a 100% sorghum had undetectable level of unsaturated fatty acids. The highest cholesterol content of 30.61 mg/100g was detected in the thigh muscles of chicken with 100% sorghum inclusion. The vitamin E content in the thigh meat of chicken subjected to diets high on maize content were significantly higher (P ≤ 0.01) than those fed on high sorghum diets. Meat from chicken fed on high low tannin sorghum meals had significantly lower (P ≤ 0.01) cholesterol content of 8.63 to 3.44 mg/100g compared to 20.10 mg/100g from diets high on maize. The present study shows that partial substitution of maize with low tannin sorghum can improve the nutritional value of broiler meat. • Chicken fed on low tannin sorghum and maize diets as the key sources of energy and meat samples collected after 8 weeks of feeding. • Cholesterol deposits higher in high maize diets. • Deposition of Vitamin E and A not affected by diet in breast meat. • Deposition of Vitamin E affected by diet on thigh meat. • Cholesterol deposition in muscles affected by levels of saturated and unsaturated fatty acids.

Changes on the Development of Rigor Mortis in Cultured Tilapia (Oreochromis niloticus) Fed with a Mixture of Plant Proteins

In recent years it has been pointed out that the feed of farmed fish has an effect on the quality of the final product. Therefore, this study evaluated the effect of fishmeal (FM) replacement by a mixture of plant protein (MPP) on the development of rigor mortis of tilapia (Oreochromis niloticus). One hundred and twenty fish at an initial average weight of 123 ± 6.3 g were fed with three extruded isonitrogenous and isolipidic 6.2% crude lipids experimental diets, in which FM were replaced by 0% (D0), 50% (D50), and 100% (D100) of MPP (soybean meal, corn meal, wheat meal, and sorghum meal). A reference diet (DC) containing FM as the main protein source was used as a control. The fish were divided into triplicate groups per dietary treatment. The experiment was conducted in a tank system at 26.8°C water temperature for 67 days. The chemical composition of experimental diets and muscle were determined. The glycogen, adenosine 5′-triphosphate (ATP) and related compounds, pH, shear force, and rigor index (RI%) were monitored during storage on ice for 48 h. The results indicated that FM replacement affected (p≤0.05) the muscle composition, where the fish fed with D100 presented the higher content of lipids and ash. Fish fed with D0 and DC presented a more pronounced onset of rigor mortis and also showed a higher IR%, a lower content of glycogen, ATP, adenosine 5′-diphosphate (ADP), adenosine 5′-monophosphate (AMP), pH, and shear force. The changes in chemical composition of muscle and other parameters evaluated indicated that FM replacement increases energy reserves (glycogen, ATP, ADP, and AMP) which delayed the onset of rigor mortis, as well as a lower pH and shear force in the muscle of tilapia. Therefore, the substitution of FM by MPP could contribute to delaying the onset of rigor mortis and with this, the quality and shelf life of tilapia could be increased.

Consumer Acceptance of Biscuits Supplemented with a Sorghum-Insect Meal.

Insects are abundant in the predominantly sub-Saharan Africa region and are generally high in protein. Wheat grain contains gluten that is vital for the quality of baked goods but does not grow well in warm regions. Partial substitution of wheat with sorghum and insect in biscuits could contribute to food security among vulnerable populations. This study identified insect types most commonly consumed by the rural Olugboja community living in the rural part of the Ikare-Akoko local government area of Ondo State, Nigeria and consumer acceptance of biscuits supplemented with a sorghum and insect meal. Whole grain sorghum meal and insect meal were blended at a ratio of 3:1 (w/w sorghum: insect). Composite biscuits were made by partially substituting wheat flour with the sorghum–insect meal at 20%, 40%, and 60% (w/w). Wheat biscuit (100%) was used as a control. Regular consumers of biscuits (n = 84) evaluated the acceptability of the biscuit samples using a five-point facial hedonic scale, which was followed by focus group discussions (FGDs) to assess consumer perceptions of the use of insect as a food source. Biscuits containing the sorghum–insect meal (mean = 4.0 ± 0.6) were more acceptable than the control (3.58 ± 0.6). The biscuits supplemented with 20% of the sorghum–insect meal were the most acceptable (mean = 4.23 ± 0.6) compared to those with higher concentrations (40% and 60%). FGDs revealed that the taste of the biscuits was an important motivation for consumers to accept insect as a food source.

Energy values and standardized ileal digestibility of amino acids in some feedstuffs for broilers

The objective of this study was to determine the energy values, standardized ileal digestibility coefficients and digestible amino acid contents of parboiled brown rice bran (PBRB), protein sorghum meal (PSM) and bread waste meal (BWM) in broiler diets. In the first trial, 144 broiler chicks at 14 days of age were evaluated in a completely randomized design involving three feedstuffs + a control diet, with six replicates and six birds per experimental unit. The total excreta collection method was adopted to determine the energy values of the feedstuffs. In the second trial, 144 broilers at 24 days of age were used in a completely randomized design involving three feedstuffs + a protein-free diet, with six replicates and six birds per experimental unit. Ileal digesta was harvested to determine the standardized ileal digestibility coefficients and digestible amino acid contents of the feedstuffs. The nitrogen-corrected apparent metabolizable energy (AMEn) values of PBRB, PSM and BWM were 2638, 2799 and 3399 kcal.kg-1, respectively. The metabolizability coefficients of AMEn (CAMEn) of PBRB, PSM and BWM were 54.13, 57.35 and 86.66%, respectively. Bread waste meal had the highest CAMEn (p &lt; 0.05) and standardized ileal digestibility of amino acids (p &lt; 0.01), followed by PBRB and PSM. The main limiting digestible amino acids in PBRB, PSM and BWM were found at the following respective levels: methionine - 0.19, 0.33 and 0.24%; lysine - 0.40, 0.46 and 0.40%; threonine - 0.25, 0.50 and 0.45%; and valine - 0.44, 0.73 and 0.48%.

INFLUENCE OF PROBIOTIC AND VITAMIN C ON THE DIGESTIVE METABOLISM OF THE QUAIL

For the detoxification of mycotoxin aflatoxin B1 in the feeding of poultry, various probiotic feed preparations based on living beneficial microorganisms and antioxidants, which significantly improve the intestinal microflora, immunity, digestibility and digestibility of nutrients feed, which has a positive effect on productivity. This article presents experimental data that suggest that to increase the growth rate, digestibility and digestibility of nutrients in the feed for quails based on barley grain, sorghum and soybean meal with a tolerant level of aflatoxin B1 should be added together probiotic Bioximin «Chicken» at a dose of 1500 g/t and vitamin C at a dose of 500 g/t feed. In comparison with the control analogues in poultry of 3 experimental groups in increasing the safety of livestock by 6 %, the average daily gain of live weight - by 12.48 % while reducing feed consumption per 1 kg of increase - by 11.84 %. Feeding probiotic and vitamin C contributed to a significant (p&lt;0.05) increase in the digestibility coefficients of dry matter by 3.08 %, organic matter - by 3.10 %, crude protein - by 3.30 %, crude fiber - by 3.06 % and BEV - by 3.33 %. Better digestibility of raw protein of feed was promoted by joint additives of probiotic and antioxidant.

RETENSI NITROGEN DAN ENERGI METABOLIS RANSUM PADA AYAM BROILER YANG MENGGUNAKAN TEPUNG SORGUM (Sorghum bicolor (L). moench) SEBAGAI PENGGANTI JAGUNG

ABSTRACTNITROGEN RETENTION AND METABOLIC ENERGY OF BROILER CHICKEN RATION USING SORGHUM MEAL (Sorghum bicolor (L). moench) AS A SUBSTITUTE FOR CORN. The aims of this study was to determine the value of Nitrogen Rretention (RN) and Nitrogen-Corrected Metabolic Energy (AMEn) rations using sorghum meal as a substitute for corn in broiler chickens. The study was carried out using 20 broiler strain Cobb aged six weeks consisting of six preliminary days and four days of data collection. Completely Randomized Design (CRD) was used which is consisting of five treatments and four replications. The treatments used are: R0 = substitute 100% corn or sorghum 0%, R1 = substitute 25% corn or sorghum 12.5%, R2 = substitute 50% corn or sorghum 25%, R3 = substitute 75% corn or sorghum 37.5%, and R4 = substitute 100% corn orsorghum 50%. The variables observed were the Nitrogen Retention value (RN) and Nitrogen Corrected Metabolic Energy (AMEn). Study showed that RN value of R0 (63.38%), R1 (65.69%), R2 (70.76%), R3 (70.77%), R4 (68.03%) and AMEn R0 (2417 , 14 Kkl / kg), R1 (2331.17 Kkl / kg), R2 (2157.87 Kkl / kg), R3 (2261.17 Kkl / kg) R4 (2290.02 Kkl / kg). he treatment gave no significant effect (P&gt; 0.05) on RN value and AMEn. Based on this study it can be concluded that sorghum flour can replace corn to 100% or use 50% in broiler ration seen from the N retention value (68.03%) and AMEn value (2290.02 Kkl / kg).Keywords : Broiler chicken, corn, metabolic energy, nitrogen retention, sorghum

Connaissances Paysannes Et Prédisposition À Adopter Une Innovation En Agro-Alimentaire : Cas Du Décorticage Mécanique Et De La Fortification En Fer Du Sorgho Dans Le Nord-Bénin

The consumption of meals prepared from unshelled sorghum derivatives is a factor favoring iron deficiency anemia among consumers. Mechanical dehulling and iron fortification of sorghum appears as a palliative solution. This study has a dual purpose. First of all, it is a question of assessing consumers' knowledge of sorghum meals with regard to anemia and its causes. Descriptive statistics have been used for this purpose. Then, it was discussed to analyze the determinants of the predisposition of these consumers to adopt mechanical dehulling and iron fortification of sorghum. To do this, the Logit econometric model was used. According to the results, anemia is caused by factors such as malaria, malnutrition, witchcraft, non-respect of fetishes, etc. In addition, households' propensity to adopt innovation is positively influenced by the income of the chef-cuisine, the perception that it has of its social status after the adoption of innovation and the compatibility of it with norms and values of the household. However, it is negatively influenced by the participation of the chef-cuisine in the experimentation phase and by the perceived complexity of the innovation.

Short communication: In vitro rumen gas production and starch degradation of starch-based feeds depend on mean particle size

Our objective was to model the effect of mean particle size (mPS) on in vitro rumen starch degradation (IVSD) and the kinetics of gas production for different starch-based feeds. For each feed, 2 batches of the same grains were separately processed through 2 different mills (cutter or rotor speed mills), with or without different screens to achieve a wide range of mPS (0.32 to 3.31 mm for corn meals; 0.19 to 2.81 mm for barley meals; 0.16 to 2.13 mm for wheat meals; 0.28 to 2.32 mm for oat meals; 0.21 to 2.36 mm for rye meals; 0.40 to 1.79 for sorghum meals; 0.26 to 4.71 mm for pea meals; and 0.25 to 4.53 mm for faba meals). The IVSD data and gas production kinetics, obtained by fitting to a single-pool exponential model, were analyzed using a completely randomized design, in which the main tested effect was mPS (n = 6 for all tested meals, except n = 7 for corn meals and n = 5 for sorghum meals). Rumen inocula were collected from 2 fistulated Holstein dairy cows that were fed a total mixed ration consisting of 16.2% crude protein, 28.5% starch, and 35.0% neutral detergent fiber on a dry matter basis. The IVSD, evaluated after 7 h of rumen incubation, decreased linearly with increasing mPS for corn, barley, wheat, rye, pea, and faba meals, and decreased quadratically with increasing mPS for the other meals. The y-axis intercept for 7-h IVSD was below 90% starch for corn, barley, and rye feeds and greater than 90% for the other tested feeds. The mPS adjustment factors for the rate of rumen starch degradation varied widely among the different tested feeds. We found a linear decrease in starch degradation with increasing mPS for barley, wheat, rye, and pea meals, whereas we noted a quadratic decrease in starch degradation for the other tested meals. Further, we observed a linear decrease in the rate of gas production with increasing mPS in each tested feed, except for pea meal, which had a quadratic relationship. For each 1 mm increase in mPS, the gas production was adjusted by -0.009 h-1 for corn, -0.011 h-1 for barley, -0.008 h-1 for wheat, and -0.006 h-1 for faba, whereas numerically greater adjustments were needed for oat (-0.022 h-1), rye (-0.017 h-1), and sorghum (-0.014 h-1). These mPS adjustment factors could be used to modify the starch-based feed energy values as a function of mean particle size, although in vivo validation is required.

Physical properties and estimated glycemic index of protein-enriched sorghum based chips.

Sorghum is a gluten-free grain and more attention has been given to the nutritional properties and recently its usage as a wheat replacement in food products. In the present work, protein-enriched sorghum based snack chips, prepared from sorghum meal with soy protein isolates and soy flour to meet the final protein content of 35.7%, were produced. The effect of varying baking powder (1.5-2.5%), dough sheet thickness (0.7-1.7mm), and baking time (6-12min) on the physical properties of the snack chips was investigated using a central composite design of response surface methodology. Under baking temperature of 160°C, with baking powder added, the water activity and puffiness of chips significantly increased. Baking time was the most significant factor for all the parameters detected except for puffiness. The optimized conditions of preparing protein-enriched sorghum chips were baking powder 2.5%, dough sheet thickness 0.7mm, and baking time 7.66min. The estimated glycemic index (eGI) of the protein-enriched sorghum chips (eGI=59.8) was significantly lower than soybean-free sorghum chips. The gluten-free protein-enriched sorghum chips developed could be considered as protein rich with lower intermediate-glycemic index classified healthy snacks and potential commercialization.

Metabolizable energy in energy food for growing pigs and crossvalidation regression models

The present study aimed to determine the apparent metabolizable energy (AME) of six corn cultivars, two sorghum cultivars and two wheat brans and to evaluate the cross-validation of predictive models of AME for corn, sorghum and wheat bran for growing pigs, as estimated from the data of chemical composition. Forty-four pigs, with an average initial weight of 24.3 kg, were distributed in a randomized block design, with 11 treatments (ten food treatments and the reference diet), four replicates and one pig per experimental unit. The reference diet was replaced by 30% for the ground corn and sorghum conditions and 20% for the wheat bran condition. The values of AME for corn, sorghum and wheat meal for pigs ranged from 3161 to 3275, 3317 to 3457 and 2767 to 2842 kcal kg-1 as a feed basis, respectively. The average metabolizability of the gross energy did not differ between the corn and sorghum cultivars, which formed a homogeneous group of food. Next, linear regression models were fitted to the 1st degree of the observed values as a function of the predicted AME, to test the hypothesis β0 = 0 and β1 = 1 in an experimental sample and 200 bootstrap samples. Fourteen predictive models had low percentages of cross-validation, ranging from 0-29.5%. The AME1A = 2.547 + 0.969ADE model was validated in experimental sample and 68% of bootstrap samples, proving its accuracy in estimating the AME of corn and sorghum from national data for growing pigs.

Contamination of fermented foods in Nigeria with fungi

This study assessed the safety and quality of some fermented foods in Nigeria. Cluster sampling was used to obtain different fermented foods: maize gruel (ogi), locust beans (iru), sorghum meal (ogi baba), dried locust beans (dried iru), African oil bean seed (ugba) and melon (ogiri) from Southwest Nigeria. Moisture content, Total Titratable Acidity, pH, and fungal diversity within each sample were determined. The identity of the isolates was established through macroscopic, microscopic and molecular biology means. The moisture content and pH of analysed samples ranged from 12 to 56% and 3.60 to 8.08, respectively. The overall data on the mycobiota of the fermented foods revealed that total fungal loads of ugba and ogiri were 1.05 × 105 and 7.9 × 105 cfu/g, respectively. Generally, fungal isolates belonged to 17 genera including Aspergillus, Fusarium, Candida, Saccharomyces and Penicillium. The dominant fungi detected were A. flavus and all analysed samples were contaminated with F. verticillioides except for ogi baba. The study led to the discovery of new fungal strains and previously unreported fungal species in the selected fermented foods. The analysed fermented foods were highly contaminated with different fungal species that could potentially be toxigenic in producing various types of mycotoxins.

Effect of Citric Acid, Phytase and Calcium Levels on the Calcium and Phosphorus Content in Egg: Yolk-Albumen and Shell, Yolk Color and Egg Quality in Diets of Laying Hens

To evaluate the effect of different levels of citric acid (CA) combined with phytase and calcium levels in the diets of laying hens on the calcium and phosphorus (P) contents of eggs: yolk-albumen and shell, yolk color and egg quality. A study was conducted with 24-week-old laying hens fed with a diet based on sorghum and soybean meal with 2.7 Mcal of EM/kg, 15% crude protein, 3.25% calcium and 0.25% available P. The levels of CA, phytase and calcium were 0%, 0.6% and 1.2%; 0 and 300 units of phytase (PHYU)/kg; and 3.0% and 3.25%, respectively. Phytase was added as an ingredient into to the diets, contributing 0.1% P and 0.3% calcium. A completely randomized factorial experiment was performed with a 3 × 2 × 2 design and 4 replications. The CA increased (P < 0.05) the P in the shell, and the combination of 1.2% CA, 300 PHYU phytase and 3.25% calcium increased (P < 0.05) the calcium in the eggshell. There was a CA × phytase × calcium interaction (P < 0.05) affecting egg yolk pigmentation; there were no differences in egg quality. It was concluded that adding CA to the diets of laying hens increases eggshell calcium content, and improves the phytase response. 1.2% CA × Phytase × 3.25% calcium significantly increases shell calcium by 0.56 g. Under the conditions of the present investigation, it would imply greater resistance of the eggs to be broken in the handling from the farm to the sales centers and consumer. Phytase interacts negatively with 0.6% CA at low calcium levels to degrade the yellow pigmentation of the yolk.

Values of Metabolizable Energy and Metabolization of Nutrients for Slow- and Fast-growing Birds at Different Ages

Two trials were performed to determine apparent metabolizable energy (AME) and AME corrected for zero nitrogen balance (AMEn) values and coefficients of apparent metabolizability of nitrogen (CAMN) and of ether extract (CAMEE) of slow- and fast-growing broilers (Isa label and Cobb, respectively) fed four different diets between 10 to 17 days or 28 to 35 days of age. The method of total excreta collection was applied. The tested feedstuffs were corn, whole corn germ (WCG), sorghum, and soybean meal (SBM). A randomized block experimental design was applied, with two treatments (breeds) and four replicates of ten animals each in the first trial, and four replicates of six animals each in the second trial. No differences in CAMN values were observed between the breeds; however, Isa Label birds presented higher CAMEE for all tested feedstuffs. The AME and AMEn values obtained in Isa Label chickens fed the corn diet were 5.75 and 3.44% higher relative tothe Cobb birds, respectively. Breed did not influence AME and AMEn values of the other tested feedstuffs. Independently of breed, age influenced the AME and AMEn values of WCG, sorghum and SBM, suggesting that birds become utilize feed more efficiently as they age.