Sonication Frequency Research Articles

Optimal Preservation of PFFs in Glycerol Enhances Suitability for Modeling Parkinson's Disease.

Injecting α-synuclein pre-formed fibrils (αSyn PFFs) into various tissues and organs involves converting monomeric αSyn into a fibrillar form, inducing extensive αSyn pathology that effectively models Parkinson's disease (PD). However, the distinct physicochemical properties of αSyn amyloid fibrils can potentially reduce their seeding activity, especially during storage. In this study, it is demonstrated that αSyn PFFs exhibit significant sensitivity to low temperatures, with notable denaturation occurring between -20 and 4°C, and gradual disassembly persisted even under storage conditions at -80°C. To mitigate this issue, a commonly used protein stabilizer, glycerol is introduced, which significantly reverses the cold-induced disassembly of PFFs. Remarkably, storing PFFs with 20% glycerol at -80°C for a month preserved their morphology and seeding activity as freshly prepared PFFs. Glycerol-stabilized αSyn PFFs resulted in compromised neuronal survival, with the extent of these impairments correlating with the formation of αSyn pathology both in vivo and in vitro, indistinguishable from freshly prepared PFFs. Storing sonicated PFFs with 20% glycerol at -80°C provides an optimal storage method, as sonication is necessary for activating their seeding potential. This approach reduces the frequency of sonication, simplifies handling, and ultimately lowers the overall workload, enhancing the practicality of using PFFs.

Quality Attributes of Ultrasound-Treated Prebiotic Fibre-Enriched Strawberry Juice

Strawberries are amongst the most desirable fruits with a rich flavour, appealing taste, high fibre content and many other health benefits. Diets rich in dietary fibre provide many health benefits. In the present work, strawberry juice was prepared in two batches, one with preservative sodium benzoate (treated) and another without (untreated). Prebiotic fibre i.e., apple pomace was added to both batches in concentrations of 5%, 8%, and 11%. Dietary fibre in both batches was analysed by enzymatic-gravimetric method. The additional analyses included pH, acidity, total soluble solids (TSS), colour, total phenolic content (TPC), antioxidant, ascorbic acid, anthocyanin, microbial and sensory parameters. Dietary fibre was increased significantly in all the treatments as well as TS, while pH and acidity were not affected. Ascorbic acid, anthocyanin, antioxidant, total phenolic content, and sensory analysis of treatment 2 (T2, 8% treated) showed the best results. The microbial load on the other hand increased more in the untreated batch. The T2 treatment of both the batches was given ultrasound treatment. The sonication temperature (20 oC), frequency (20 kHz), and power (650 W) were kept the same, and the time was varied (0, 15, 30, 45, and 60 minutes). Dietary fibre showed a slight increase as fibre became more soluble by cavitation in sonication while pH acidity and TSS were not significantly affected. Anthocyanin increased, but only at lower sonication times. Antioxidants, total phenols, and colour and sensory parameters were significantly improved with sonication time. Similarly, the microbial load was reduced significantly.

Modeling ultrasound modulation of neural function in a single cell

BackgroundLow intensity ultrasound stimulation has been shown to non-invasively modulate neural function in the central nervous system (CNS) and peripheral nervous system (PNS) with high precision. Ultrasound sonication is capable of either excitation or inhibition, depending on the ultrasound parameters used. On the other hand, the mode of interaction of ultrasonic waves with the neural tissue for effective neuromodulation remains ambiguous. New methodHere within we propose a numerical model that incorporates the mechanical effects of ultrasound stimulation on the Hodgkin-Huxley (HH) neuron by incorporating the relation between increased external pressure and the membrane induced tension, with a stress on the flexoelectric effect on the neural membrane. The external pressure causes an increase in the total tension of the membrane thus affecting the probability of the ion channels being open after the conformational changes that those channels undergo. ResultsThe interplay between varying the acoustic intensities and frequencies depicts different action potential suppression rates, whereby a combination of low intensity and low frequency ultrasound sonication proved to be the most effective in modulating neural function.Comparison with Existing Methods: Our method solely depends on the HH model of a single neuron and the linear flexoelectric effect of the dielectric neural membrane, when under an ultrasound-induced mechanical strain, while varying the ion-channels conductances based on different sonication frequencies and intensities. We study the effect of ultrasound parameters on the firing rate, latency, and action potential amplitude of a HH neuron for a better understanding of the neuromodulation modality of ultrasound stimulation (in the continuous and pulsed modes). ConclusionsThis simulation work confirms the published experimental data that low intensity and low frequency ultrasound sonication has a higher success rate of modulating neural firing.

Ultrasound-assisted fortification of yellow sweet potato (Ipomoea batatas) with iron and ascorbic acid

The objective of this study was to evaluate the effect of ultrasound on the incorporation of iron and ascorbic acid (AA) in sweet potato (Ipomoea batatas) and to optimize the process parameters to obtain a fortified food. The incorporation was carried out using cubes of sweet potato submerged in 0.1% m/v ferrous sulfate and 1% m/v AA solutions, treated at different times and sonication frequencies (37 and 80 kHz), at 100 watts of power and 30 ± 5°C. ANOVA and Tukey’s test at 5% significance were applied to establish significant differences and the process was evaluated using a factorial design. The results revealed that the application of ultrasound influences the content of iron and AA, incorporating greater amount of iron and AA compared to samples not treated with ultrasound. Similarly, longer times led to higher incorporation of iron and AA content in sweet potatoes; the frequency was not statistically significant. The highest iron content was 105.91 ± 0.03 mg/100 g and for AA, it was 392.65 ± 4.84 mg AAE/100 g. The defined ultrasonic process conditions produced an increase of 4928.99 and 610.65%, respectively, in iron and AA content in sweet potato.

Effect of ultrasonic frequency on the structural and functional properties of pea protein isolation.

Pea protein, as a by-product of peas (Pisum sativum L.), is rich in a variety of essential amino acids that can meet the body's protein needs and is a valuable source of protein. Since the function of pea protein is closely related to its structure, pea protein has been subjected to different modifications in recent years to improve its application in food and to develop new products. The effects of sonication frequency (primary and secondary time) on pea protein isolate's (PPI's) structural and functional properties were investigated. Sodium dodecyl sulfate polyacrylamide gel electrophoresis demonstrated that different sonication frequencies at the same power (600 W) treatment had no effect on PPI's molecular weight. Fourier-transform infrared spectroscopy revealed that treatment at different sonication frequencies caused secondary structural changes in PPI. The particle size distribution, foaming, stability, surface hydrophobicity, emulsification, and oxidation resistance of PPI were improved after primary and secondary sonication, but secondary sonication was not more effective than primary sonication for an extended period of time. Overall, ultrasound is able to improve the structural and functional properties of pea proteins within a suitable range. It provides a theoretical basis for elucidating the modification of the structure and function of plant proteins by ultrasound and lays the foundation for the development of plant proteins in food applications as well as development. © 2023 Society of Chemical Industry.

Application of the general rate law model to the sonolytic degradation of nonvolatile organic pollutants in aqueous media

The pseudo-first and pseudo-second order equations have been the most commonly used models to characterize the sonolytic disappearance kinetics of nonvolatile pollutants in aqueous media. In this work, the general rate law model, i.e., pseudo-nth order kinetics equation, was applied for the first time to the sono-decomposition of different nonvolatile organic pollutants, naphthol blue black (NBB), furosemide (FSM), 4-isopropylphenol (4-IPP), and rhodamine B (RhB), in water. It was shown that the general rate law for a chemical reaction would apply to the kinetics of sonochemical decomposition. It is not feasible to set the order of ultrasonic pollutant degradation kinetics to pseudo-first or pseudo-second, as is typically used in numerous studies. The sonochemical oxidation reaction has a fractional order, the order is often non-integer, which frequently indicates a complex sonolytic decomposition reaction mechanism. The degradation mechanism of NBB and RhB does not change with the initial substrate concentration. They are ultrasonically degraded by hydroxyl radicals both in the bulk liquid solution and at the liquid/bubble interfacial layer. The destruction mechanism of FSM and 4-IPP changes as the initial contaminant concentration changes. At low initial substrate concentrations, these pollutants are oxidized mainly by reaction with hydroxyl radicals in the bulk liquid solution and at the interfacial shell of the cavitation bubbles. At high initial substrate concentrations, FSM and 4-IPP are degraded by thermal destruction in the liquid/bubble interfacial layer and by •OH radicals both in the bulk liquid solution and at the liquid/bubble interfacial layer. Additionally, the pseudo-nth order model predicts very well the sonolytic degradation at various sonication frequencies and intensities. The general rate law expression should be used to assess the real kinetics order of the sonolytic destruction process without any predetermined assumptions or constraints.

Effect of Sonication Frequency and Power Intensity on the Disruption of Algal Cells: Under Vacuum and Non-Vacuum Conditions

The presence of algae caused by anthropogenic eutrophication in water has become a severe environmental issue. Various treatment options for algae removal have been developed, such as filtration, coagulation, sedimentation, flotation, algicides, ozone, and photolysis. However, these technologies are complex, expensive, consume considerable amounts of various chemicals, and may cause further pollution (i.e., by-product formation). Ultrasonic exposure is an alternative method for removing algae from water that is environmentally friendly (i.e., no addition of chemicals) and almost unaffected by any turbidity in the water. In this study, process optimization of ultrasonication (e.g., by adjusting frequency, power intensity, and exposure time) for the removal of alga was tested under vacuum and non-vacuum conditions. Experiments were conducted on a batch of algae solution in a clear glass tube ultrasonicated by a 20 kHz transducer for 180 minutes. The tube was depressurized up to -67 N/m2 in a depressurizing chamber. The data was collected at transducer depths of 0.06, 0.13, and 0.19 m. It was concluded that the optimum condition (i.e., 92% algal cell disruption) was achieved when the power intensity was 7 kWh/m3, under vacuum conditions, at a frequency of 20 kHz and 180 minutes of exposure time. Higher power intensity gave higher energy for cell disruption, moreover by depressurizing the air above the algae solution, the lysis effect for algae reduction increased from 20% to 70% compared to the non-depressurized system due to higher cavitation bubble production. In addition, the depth of the transducer was another factor that could increase the lysis of the algae water. Therefore, this technology has future potential application for algae removal from water.

Ultra-low temperature sono-assisted CO2 stripping/ carbon-rich solvent regeneration using different ultrasonic frequencies

ABSTRACT Carbon dioxide emission from anthropogenic sources plays a major role in global warming and climate change. Conventional amine-based carbon capture is matured technology that has limitations on high-temperature use imposed by solvent degradation and solvent loss during CO2 stripping/regenerating solvent. The present experimental investigation involves the application of tank-type (bath-type) sonication for CO2 stripping from aqueous carbon-rich 30 wt% MEA solvent in a low temperature-controlled environment, using ultrasonic frequencies of 25 kHz (cavitation-dominant), 360 kHz (streaming-dominant), and 58/132 kHz dual-mode (combined phenomena). The results are analyzed to understand the effects of carbon loading, CO2 stripping efficiency, stripping rate, temperature profile, and energy demand. The stripping rate is higher in the low-temperature range of 6°C to 12°C for all the frequencies due to the sonication effect. In the mid setpoint range of 15°C to 30°C, the CO2 stripping rate is lower due to the combination of sonication and temperature effect, and when the temperatures increase from 40°C to 45°C, the stripping rate increases gradually due to additional temperature effect. A high cyclic capacity of 1.12 mol CO2/kg. was observed for 360 kHz frequency at 12°C. The lowest energy of 3.94 kJ/mol CO2 was achieved for 360 kHz at a setpoint of 12°C. Factors such as sonication frequency, CO2 loading (mol CO2/mol soln.), temperature, and nominal power of ultrasonics play an important role in determining the sensible energy required for CO2 stripping. Specifically, CO2 stripping at the low temperature set point of 6°C and 12°C is found to be promising in all frequencies, indicating that CO2 stripping is primarily promoted by sonication, rather than by temperature.

Resonant frequency of coronavirus: The tensegrity approach

COVID-19 is a worldwide health hazard. In discovering a fully efficient vaccination for continually evolving viruses, alternatives such as the sonication treatment methods, which have seen encouraging outcomes in disinfection and medical therapies, are worth exploring. Such treatments incapacitate microbes or diseased cells by selectively invoking large deformation at the resonant frequency that initiates structural failure. Nevertheless, due to biodiversity, the precise range of resonant frequencies for different biological bodies can be widely varying, its determination of which is best achieved with the cost-effective computational simulation approach. The paper proposes, therefore, the numerical determination of the resonant frequency of the coronavirus employing the tensegrity method, due to its efficacy in modeling various biomechanical behaviors of different biological bodies and systems. Initiating from successfully verifying the resonant frequency with existing findings, the model then determines the operative sonication frequencies range for the resonant state of the virus. At these sonication frequencies, the modal analysis exhibits a large structurally destructive deformation of the coronavirus. For practical convenience, the operative range of sonication resonant frequencies of the coronavirus has been mapped alongside healthy human cells. These findings offer an alternative technological avenue in combating the COVID-19 progressive threat.

Characterisation of microneedle replication and flow behaviour in ultrasonic micro-injection moulding through design of experiments

This research investigates the impact of process parameters on ultrasonic micro-injection moulding (u-μIM) by studying microneedle replication efficiency, temperature evolution during moulding, melt flow consistency and sonication behaviour. The experimentation comprises of a full-factorial design of experiments using three process variables: sonication time, mould temperature and injection force. The current study presents that; (i) mould temperature was found to be the most influential parameter to tune and optimise micro-feature replication, (ii) consistent ultrasonic micromoulding flows can be achieved using higher mould temperatures, (iii) injection force is a key parameter influencing sonication frequency, a critical aspect of the ultrasonic method to obtain a repeatable manufacturing process. These outcomes will be a datum during development of the manufacturing processes for specific products using the ultrasonic method. The research presented here takes a unique approach to characterise the ultrasonic method for the development of this novel technique, particularly for microneedle patch and medical device production. With this increased level of understanding of u-μIM process characteristics, the method can become an energy efficient and cost-effective alternative to conventional micro-injection moulding processes for delivering high-quality, miniature components.

Effect of Ultrasonic Pretreatment on the Far-Infrared Drying Process and Quality Characteristics of Licorice.

In this paper, the effects of different ultrasonic pretreatment processes on the far-infrared drying characteristics, quality indexes, and microstructure of licorice are evaluated. The results showed that ultrasonic pretreatment, combined with far-infrared drying, significantly reduced the drying time and moisture content of licorice compared with those of the control group. The highest total flavonoid content was obtained at an ultrasound power of 80 W. The total phenolic content (0.686 mg gallic acid equivalent/g) was higher than that in the control group, the increase was 19.4%, and its content was the highest at the sonication frequency of 20 kHz. The antioxidant capacity tended to increase and then decrease with the increase in sonication time, sonication power, and sonication frequency, and was the highest at 30 min of sonication. The soluble sugar content (31.490 mg glucose equivalent/g) was the highest at 30 kHz and 30 min. Observation of the microstructure revealed that the surface structure of the ultrasonic pretreated licorice slices changed significantly, forming more micropore channels, which facilitated the mass heat transfer during the drying process. In conclusion, ultrasonic pretreatment can significantly improve the quality of licorice tablets and significantly reduce the time required for subsequent drying. The combination of pretreatment parameters of 60 W ultrasonic power and 40 kHz ultrasonic frequency for 30 min was found to be an optimal combination of pretreatment parameters; therefore, this study may provide a technical reference for the industrialization of licorice drying.

Sonochemical formation of peroxynitrite in water: Impact of ultrasonic frequency and power

There is a lack of literature on peroxynitrite formation due to sonolysis of aerated water. In this work, the impact of sonication parameters, frequency and power, on ultrasonic peroxynitrite production in aerated alkaline water was investigated. Peroxynitrite formation was clearly established with undeniable evidence at all the tested frequencies in the range of 516–1140 kHz with a typical G-value (energy-specific yield) of 0.777 × 10−10, 0.627 × 10−10, 0.425 × 10−10 and 0.194 × 10−10 mol/J at 516, 558, 860 and 1140 kHz, respectively. The ultrasonication frequency has a direct impact on the sonochemical peroxynitrite production. Increasing the ultrasonication frequency in the interval 321–1140 kHz reduces peroxynitrite formation. The most practical sonochemistry dosimetries, including hydrogen peroxide production, triiodide dosimetry, Fricke dosimetry, and 4-nitrocatechol formation, were compared with the sonochemical efficiency of the reactors used to produce peroxynitrite. The G-value, energy specific yield, for the tested dosimetries was higher than that for peroxynitrite formation, regardless of frequency. For all chemical dosimetries investigated, the same trend of frequency dependence was found as for peroxynitrite generation. The influence of ultrasonication power on peroxynitrite formation by sonication at diverse frequencies in the interval 585–1140 kHz was studied. No peroxynitrite was formed at lower acoustic power levels, regardless of frequency. As the frequency increases, more power is required for peroxynitrite formation. The production of peroxynitrite increased as the acoustic power increased, despite the frequency of ultrasonic waves. Ultrasonic power is a key factor in the production of peroxynitrite by sonolysis. Since peroxynitrite is uniformly distributed in the bulk solution, peroxynitrite-sensitive solutes can be transformed both in the bulk of the solution and in the surfacial region (shell) of the cavitation bubble. The formation of peroxynitrite should be taken into account in sonochemistry, especially at higher pH values. Ultrasonic peroxynitrite formation in alkaline solution (pH 12) can be considered as a kind of chemical dosimetry in sonochemistry.

Ultrasound frequency sonication facilitates high-throughput and uniform dissociation of cellular aggregates and tissues.

A sample preparation step involving dissociation of tissues into their component cells is often required to conduct analysis of nucleic acids and other constituents from tissue samples. Frequently, the extracellular matrix and cell-cell adhesions are disrupted via treatment with a chemical dissociating reagent or various mechanical forces. In this work, a new, high-throughput, multiplexed method of dissociating tissues and cellular aggregates into single cells using ultrasound frequency bath sonication is explored and characterized. Different operating parameters are evaluated, and a treatment protocol with potential for uniform, high-throughput tissue dissociation is compared to the existing best chemical and orbital plate shaking protocol. Metrics such as percent dissociation, cellular recovery, average aggregate size, proportion of various aggregate sizes, membrane circularity, and cellular viability are subsequently assessed and found to be favorable. In optimized conditions, 53 ± 8% of 1 mm biopsy cores are dissociated within 30 min using sonication alone, surpassing leading high-throughput orbital plate shaking techniques five-fold. Chemical digestion is also 2 times more effective when complexed with sonication rather than orbital plate shaking. RNA content, quality, and expression are found to be superior to the standard protocol in terms of transcriptional preservation.

Robust Biocompatible Fibers from Silk Fibroin Coated MXene Sheets

AbstractConductive fibers are needed for the development of flexible electronic and biomedical devices. MXene fibers show great promise for use in such applications because of their high conductivity. Current literature on MXene fiber development highlights the need for improving their mechanical properties and investigation of biocompatibility. Here the use of silk fibroin biopolymer as a MXene formulation additive for the production of MXene fibers is studied. It is found that the favorable silk fibroin–MXene interactions resulted in improved durability, withstanding up to 1 h of high frequency sonication in buffered solutions. Furthermore, fibers with ≈5 wt% silk fibroin displays interesting properties including high conductivity (≈3700 S cm−1), high volumetric capacitance (≈910 F cm−3), and non‐cytotoxicity toward THP‐1 monocytic cells. The results presented here provide an important insight into potential use of MXene fibers in flexible electronics and biomedical applications.

Reproduction of Nanofluid Synthesis, Thermal Properties and Experiments in Engineering: A Research Paradigm Shift

The suspension of different nanoparticles into various conventional thermal fluids to synthesize nanofluids has been proven to possess superior thermal, optical, tribological, and convective properties, and the heat transfer performance over conventional thermal fluids. This task appears trivial but is complicated and significant to nanofluid synthesis and its subsequent utilization in diverse applications. The stability of mono and hybrid nanofluids is significantly related to stirring duration and speed; volume, density, and base fluid type; weight/volume concentration, density, nano-size, and type of mono or hybrid nanoparticles used; type and weight of surfactant used; and sonication time, frequency, mode, and amplitude. The effects of these parameters on stability consequently affect the thermal, optical, tribological, and convective properties, and the heat transfer performance of nanofluids in various applications, leading to divergent, inaccurate, and suspicious results. Disparities in results have inundated the public domain in this regard. Thus, this study utilized published works in the public domain to highlight the trend in mono or hybrid nanofluid formulation presently documented as the norm, with the possibility of changing the status quo. With the huge progress made in this research area in which a large quantum of different nanoparticles, base fluids, and surfactants have been deployed and more are still emerging in the application of these advanced thermal fluids in diverse areas, there is a need for conformity and better accuracy of results. Reproduction of results of stability, thermal, optical, tribological, anti-wear, and fuel properties; photothermal conversion; and supercooling, lubrication, engine, combustion, emission, thermo-hydraulic, and heat transfer performances of formulated mono or hybrid nanofluids are possible through the optimization and detailed documentation of applicable nanofluid preparation parameters (stirring time and speed, sonication duration, amplitude, mode, frequency, and surfactant concentration) employed in formulating mono or hybrid nanofluids. This proposed approach is expected to project a new frontier in nanofluid research and serve as a veritable working guide to the nanofluid research community.

Influence of Preparation Characteristics on Stability, Properties, and Performance of Mono- and Hybrid Nanofluids: Current and Future Perspective

Nanofluids (NFs) synthesized via the suspension of diverse nanoparticles into conventional thermal fluids are known to exhibit better thermal, optical, tribological, and convective properties, photothermal conversion, and heat transfer performance in comparison with traditional thermal fluids. Stability is pivotal to NF preparation, properties, performance, and application. NF preparation is not as easy as it appears, but complex in that obtaining a stable NF comes with the harnessing of different preparation parameters. These parameters include stirring duration and speed, volume, density, base fluid type, weight/volume concentration, density, nano-size, type of mono or hybrid nanoparticles used, type and quantity of surfactant used, and sonication time, temperature, mode, frequency, and amplitude. The effect of these preparation parameters on the stability of mono and hybrid NFs consequently affects the thermal, optical, rheological, and convective properties, and photothermal conversion and heat transfer performances of NFs in various applications. A comprehensive overview of the influence of these preparation characteristics on the thermal, optical, rheological, and properties, photothermal conversion, and heat transfer performance is presented in this paper. This is imperative due to the extensive study on mono and hybrid NFs and their acceptance as advanced thermal fluids along with the critical importance of stability to their properties and performance. The various preparation, characterization, and stability methods deployed in NF studies have been compiled and discussed herein. In addition, the effect of the various preparation characteristics on the properties (thermal, optical, rheological, and convective), photothermal conversion, and heat transfer performances of mono and hybrid NFs have been reviewed. The need to achieve optimum stability of NFs by optimizing the preparation characteristics is observed to be critical to the obtained results for the properties, photothermal conversion, and heat transfer performance studies. As noticed that the preparation characteristics data are not detailed in most of the published works and thus making it mostly impossible to reproduce NF experimental studies, stability, and results; future research is expected to address this gap. In addition, the research community should be concerned about the aging and reusability of NFs (mono and hybrid) in the nearest future.

Optimization of ultrasonication-assisted extraction conditions using RSM-I-Optimal experimental design to recover vitamin D2 and K1 from selected green leafy vegetable samples

This study employed the response surface methodology to optimize the extraction conditions for recovering vitamins D2 and K1 from green leafy vegetables using ultrasonication-assisted extraction. The vitamin content was determined using an Accucore C18 column and a UPLC-Q-ToF/MS method. An RSM-I-Optimal design was used for designing the experiment to find the best combination of solvent level (mL), sonication time (min), sonication frequency (kHz), and temperature (°C). The experimental data from a 25-sample set were fitted to a second-order polynomial equation using multiple regression analysis. The extraction models had R2 values of 0.895 and 0.896, respectively, and the probability values (p < 0.0001) indicated that the regression model was highly significant. The optimal extraction conditions were: solvent level of 65 mL, sonication time of 45 min, sonication frequency of 70 kHz, and temperature of 45 °C. Under these conditions, the predicted recovery (%) values for vitamins D2 and K1 were 90.7% and 90.4%, respectively. This study has the potential to use the reported extraction method for routine quantification of vitamins D2 and K1 in the laboratory using UPLC-Q-ToF/MS.

Utilization of focused ultrasound for opening of the blood-nerve barrier

Objective. Focused ultrasound (FUS) use with and without microbubbles (MB) for investigation of the blood-nerve barrier (BNB) within the peripheral nervous system (PNS) has been performed in this study. We evaluate the feasibility of BNB opening in a rodent sciatic nerve model by direct vision FUS treatment and provide preliminary results of magnetic resonance guided FUS (MRgFUS). Approach. Twenty rodent bilateral sciatic nerves were investigated. Rodents were treated using a benchtop FUS system to directly visualize nerve FUS studies. Definity MB, Evans blue dye (EB) and latex micro beads were injected during studies. Selected animals underwent further compound muscle action potential (CMAP) studies. Sonication peak pressure (MPa), width, duty-cycle and duration as well as MB concentration were varied to investigate effective pressure threshold. Further preliminary MRgFUS studies were performed on selected animals. Immunohistochemistry and histological analysis under florescent microscopy were performed at termination of experiments to verify treatment outcomes. Main results. Three ultrasound pressures and three microbubble concentrations at a single sonication frequency (476.5 kHz) were performed under direct open targeting. Histological analysis demonstrated nerve internal architecture disruption at 1.2 MPa with 166.7 μl kg−1 while 0.3 MPa, with 40 μl kg−1 MB concentration was the lower threshold for consistently observed disruption of the BNB without anatomical microarchitecture disruption. EB leakage was confirmed at the target region in histological evaluation of nerve following MB injection and FUS sonication. Supra-harmonic emissions were detected during FUS exposures following MB injection but not at baseline reference, indicating effective MB response and stable cavitation. CMAP amplitudes showed delayed onset latency and lower amplitudes in sonicated nerves compared to control nerves without evidence of complete conduction block, suggesting a transient BNB disruption, while at lower limit pressure subtle conduction changes were observed. In MRgFUS, targeted nerves demonstrated further contrast agent leak as well as supra-harmonic frequency detection. Significance. Opening of the BNB in the PNS was achieved using FUS and MB in a rodent model. Ongoing work aims to refine FUS parameters for drug delivery into the nerve after experimental transient BNB disruption.

Demystifying the role of graphene nanoplatelets percentage and sonication duration on the mechanical properties of the glass fabric/graphene nanoplatelets hybrid nano‐composite

AbstractIn this study, the effect of sonication time on the mechanical properties of graphene nanoplatelets (GnPs) glass fiber composites were discussed. The Sonication process is one of the best techniques to ensure uniform dispersion of nanofillers into the polymer matrix; otherwise, they form agglomerates of multilayers. To fulfill the massive demand for mass production, ultrasonic bath‐type sonication were deployed. In this study, 0, 1, 2, 3, 4, and 5 wt% of GnPs were added with epoxy at different sonication durations, precisely 0, 20, 40, and 60 min. Other sonication process parameters were kept constant with a sonication frequency of 28 KHz. Bidirectional glass fabric materials was used as reinforcement material in the fabrication of the laminates. In total, 21 laminates were fabricated using the hand lay‐up technique in various amalgamations. The tensile and flexural specimens were cut from the laminates as per the standards to determine their mechanical properties. The scanning electron microscope (SEM) images were used for the structural analysis of the glass fiber reinforced GnPs/epoxy composite. Surface morphology of GnPs becomes more wrinkled, resulting in a stronger graphene/epoxy matrix interface, enhancing the tensile strength and tensile modulus performance of the nano‐composites increases up to 37.12% and 66.30%, the flexural strength and flexural modulus performance of the nano‐composites increases up to 55.74% and 91.23% respectively. Based on the investigations, the influence of the GnPs percentage and the sonication duration for the preparation of the nano‐composites with the help of technique for order of preference by similarity to ideal solution (TOPSIS) optimization technique.

Optimization of a Green Extraction of Polyphenols from Sweet Cherry (Prunus avium L.) Pulp

This work focused on the optimization of the ultrasound (US) extraction of polyphenols from sweet cherry pulp by monitoring cyanidin-3O-rutinoside, quercetin-3O-rutinoside, and trans-3-O-coumaroylquinic acid, representing the main anthocyanin, flavonol, and hydroxycinnamate, respectively, identified in the extracts through chromatographic analyses (HPLC-DAD), as output variables. The optimization was performed following a two-level central composite design and the influence of the selected independent variables (i.e., extraction time and solid to solvent ratio) was checked through the response surface methodology. The maximum recovery of the phenolic compounds was obtained at 3 min and 0.25 g/mL in water/ethanol (1:1, v/v) at a set temperature (25 °C), sonication power (100 W), and sonication frequency (37 kHz). Subsequent validation experiments proved the effectiveness and reliability of the gathered mathematical models in defining the best ultrasound-assisted extraction conditions.