Solitary Manner Research Articles

A Study on the Impact of Technological Advancements Towards Product Development in Intellipaat

Telecommuting involves doing regular work from home. This arrangement may be temporary or permanent, part time or full time or a set of portions of work week. Telecommunications usually relies on communication technology like a telephone, fax machine etc. Sting an equitable balance of time among family, career and our own well-being has become increasingly complex. Unfortunately, the average daily commute is lengthening as both urban congestion and suburban sprawl continue to grow, further jeopardizing our valuable time. Telecommuting provides relief from these issues. On the other hand, social media provides an important benefit for the telecommuter. At the office, gathering at communal areas fosters workplace relationships, encourages informal brainstorming, and helps clear and refresh the mind. While working at home in a more solitary manner, social media tools like Facebook, Twitter, LinkedIn and especially internal communities connect and empower telecommuters to trade information and questions with company and industry colleagues—regardless of their location. are forcing an unprecedented rationalization of resources. Improved productivity has thus become a concern of all organizations, both public and private.

Protein Interactome of Amyloid-β as a Therapeutic Target.

The amyloid concept of Alzheimer's disease (AD) assumes the β-amyloid peptide (Aβ) as the main pathogenic factor, which injures neural and other brain cells, causing their malfunction and death. Although Aβ has been documented to exert its cytotoxic effect in a solitary manner, there is much evidence to claim that its toxicity can be modulated by other proteins. The list of such Aβ co-factors or interactors includes tau, APOE, transthyretin, and others. These molecules interact with the peptide and affect the ability of Aβ to form oligomers or aggregates, modulating its toxicity. Thus, the list of potential substances able to reduce the harmful effects of the peptide should include ones that can prevent the pathogenic interactions by specifically binding Aβ and/or its partners. In the present review, we discuss the data on Aβ-based complexes in AD pathogenesis and on the compounds directly targeting Aβ or the destructors of its complexes with other polypeptides.

Mediating Role of Customer Relationship Management between Buying Behavior and Critical Risk Factors via Digital Buying

Purpose: To examine the mediating effect of Customer Relationship Management (CRM) amid Digital Buying Behaviour (DBB) and Risk Factors (RF). Methods: The structured questionnaire was adopted to analyse critical factors of DBB, RF and CRM. The instrument was designed and validated with the help of exploratory factor analysis and confirmed the items by confirmatory factor analysis. The model fitness is verified. The reliability of the scale was tested along with CR. The field testing is done to remove ambiguity. The nativity changes were incorporated to align the current study. The responses were collected using convenience sampling method through the e-mail survey and 410 valid responses (digital consumers) considered. The data were collected during August – November 2022. Quantitative approach is used to examine both directions of the considered variables. First, the direct effect of DBB on RF is examined. Secondly, the direct effect of CRM is studied. Further, mediating effect of CRM in relationship amid DBB and RF is established. Findings: The DBB is significant and directly connected with RF (b = 0.952, p < .001), CRM is significant and positively allied with RF (b = 0.471, p < .001). DBB is significant and positively related to CRM (b = 0.779, p < 0.001), after removal of mediating variable (CRM), b weight of DBB is reduced from 0.952 to 0.703. Sobel test is steered to confirm the significance of the mediation effect of CRM, test statistic (z= 3.09, p <.001). Thus, CRM act as a partial mediator amid DBB and RF. Novelty: The present study integrates DBB, RF and CRM features associated in digital buying in solitary manner unlike sliced approach. The study of CRM role especially in digital buying is a uniqueness of the present research. Further, studying the partial mediating effect of CRM amid DBB and RF itself is a new attempt. Keywords: Digital Buying Behaviour (DBB); Risk Factors (RF); Customer Relationship Management (CRM); Mediating Effect; Confirmatory Factors Analysis; Sobel Test

MIXOMA ATRIAL: UM RELATO DE CASO

Introdução: Os mixomas são tumores cardíacos que em sua grande maioria possuem histogênese benigna e apresentam-se de maneira solitária. Localizam-se em 75% dos casos do lado esquerdo e são raros, com uma incidência menor que 0,1% e prevalência em adultos entre a terceira e sexta décadas de vida, predominando em mulheres. Embora a natureza da maioria desses tumores seja de caráter benigno, é importante diagnosticar e iniciar o tratamento com remoção cirúrgica precocemente, pois podem surgir complicações desfavoráveis como acidentes embólicos e levar a mortes súbitas. Objetivo: O propósito deste artigo é analisar um caso clínico de mixoma atrial e seus respectivos exames que estão sob posse do paciente, abordando as manifestações da doença, diagnóstico e tratamento. Com isso, é possível auxiliar em diagnósticos futuros mais precisos, uma vez que a intervenção precoce é imprescindível para vitalidade do paciente.

Kedudukan Hukum Al-Sunnah dalam Al-Qur’an

Abstract: the legal position of al-sunnah (prophet tradition) is as the source of Islamic law. However, each has distinction in term of transmission. While all verses of the Quran were transmitted successively (tawatur), not many prophet traditions are successive. Instead, they are reported in solitary manner. Thus, further meticulous examination of its authenticity is a must. The examination involves the number of transmitters as well as their personality. Therefore, the legal position of al-sunnah vis-à -vis the Quran comes in three functions: Firstly, the position of al-Sunnah as source of Islamic law secondary to the Quran and functions to elaborate the general meaning of the Quran. Secondly, al-Sunnah interprets verses of the Quran’ Thirdly, al-Sunnah perform as further interpretation for the Quran in term of bayân al-mujmal, bayân taqyîd al-muthlaq, bayân al-takhshish al-‘âm, dan bayân tawdhîh al-musykil.&#x0D; Key words: al-sunnah, the Quran, source of Islamic law

Experimental evidence of socio-spatial intolerance between female roe deer

Female roe deer tend to live in mixed-sex groups in winter but in solitary manner on reduced home ranges in spring. This tendency is commonly related in the literature to the rearing of hiding fawns. We studied the socio-spatial relationships between six female roe deer, introduced at 1 year of age into a partly wooded 14.2-ha enclosure. Animals were monitored with GPS telemetry collars for a period of 3 years, a male being added at the end of the 2nd year. Females did not share large overlapping home ranges. In the absence of any male and offspring, they spread out in May–June (birthing season) and in July-August (rutting season). During these seasons, the size and overlap of their home ranges were minimal, while the mean distance between the arithmetic centres of their respective locations reached its maximum value and was greater than expected under the hypothesis that the females positioned their ranges independently of one another. Related females were not preferentially associated beyond 2 years of age. Moreover, the introduction of a male affected this spatial organisation only slightly. Overall, the socio-spatial organisation of female roe deer appears to be almost independent of their reproductive status, and very similar to that of males, as is the case in a number of other small-bodied ruminants.

Cellular contractility changes are sufficient to drive epithelial scattering

Epithelial scattering occurs when cells disassemble cell–cell junctions, allowing individual epithelial cells to act in a solitary manner. Epithelial scattering occurs frequently in development, where it accompanies epithelial–mesenchymal transitions and is required for individual cells to migrate and invade. While migration and invasion have received extensive research focus, how cell–cell junctions are detached remains poorly understood. An open debate has been whether disruption of cell–cell interactions occurs by remodeling of cell–cell adhesions, increased traction forces through cell substrate adhesions, or some combination of both processes. Here we seek to examine how changes in adhesion and contractility are coupled to drive detachment of individual epithelial cells during hepatocyte growth factor (HGF)/scatter factor-induced EMT. We find that HGF signaling does not alter the strength of cell–cell adhesion between cells in suspension, suggesting that changes in cell–cell adhesion strength might not accompany epithelial scattering. Instead, cell–substrate adhesion seems to play a bigger role, as cell–substrate adhesions are stronger in cells treated with HGF and since rapid scattering in cells treated with HGF and TGFβ is associated with a dramatic increase in focal adhesions. Increases in the pliability of the substratum, reducing cells ability to generate traction on the substrate, alter cells׳ ability to scatter. Further consistent with changes in substrate adhesion being required for cell–cell detachment during EMT, scattering is impaired in cells expressing both active and inactive RhoA mutants, though in different ways. In addition to its roles in driving assembly of both stress fibers and focal adhesions, RhoA also generates myosin-based contractility in cells. We therefore sought to examine how RhoA-dependent contractility contributes to cell–cell detachment. Inhibition of Rho kinase or myosin II induces the same effect on cells, namely an inhibition of cell scattering following HGF treatment. Interestingly, restoration of myosin-based contractility in blebbistatin-treated cells results in cell scattering, including global actin rearrangements. Scattering is reminiscent of HGF-induced epithelial scattering without a concomitant increase in cell migration or decrease in adhesion strength. This scattering is dependent on RhoA, as blebbistatin-induced scattering is reduced in cells expressing dominant-negative RhoA mutants. This suggests that induction of myosin-based cellular contractility may be sufficient for cell–cell detachment during epithelial scattering.

The unilocular fat-cell graft

Autologous fat grafting has become a common procedure for augmenting soft tissue. However, there are still some problems with resorption and cyst formation after injection, which mainly arise from insufficient nourishment of the transplanted fat tissues. In this study, using a mouse model, we enzymatically digested fat tissues into unilocular fat cells, and then transplanted the unilocular fat cells by sub-dermal injection to allow the transplanted cells to easily spread within the injected area. Fat tissue was harvested from a green fluorescent protein transgenic mouse (C57BL/6), and the optimal digestion time was determined to be 30 min. The fat cells were then injected into the sub-dermal layer of the head skin of a C57BL/6 mouse. As a control, minced fat without digestion was also injected. The animals were sacrificed immediately after injection and on days 1, 2 and 3, as well as at weeks 1, 2 and 4 after injection, and the recipient skins were collected for microscopic observation. The unilocular fat cells were observed to spread in a solitary manner among the recipient tissues, and no necrotic areas or cysts were observed. The minced-fat-graft control showed central necrosis in the transplanted region. In addition, the minced fat tissue needed to be injected with an 18-gauge syringe, but the unilocular fat cells could be injected with a 26-gauge syringe. Thus, the unilocular fat-cell graft was determined to be a superior alternative to conventional fat grafts.

Adaptations in phalangiid harvestmen Gyas annulatus and G. titanus to their preferred water current adjacent habitats

Abstract Gyas annulatus and G. titanus (Arachnida: Opiliones: Phalangiidae) were studied comparatively with special interest in their hypogean ecophase. Direct comparisons were made in two caves where both species overwintered, and in the laboratory. Their chilling hardiness was tested, and the dynamics of lipid and glycogen stocks was studied. Juveniles overwinter inactive in physically unstable stone accumulations close to water currents, and in water- and wet caves. In caves, G. annulatus prefers colder microhabitats (at –0.1 to 2.1 °C) near the entrances and close to water surfaces while G. titanus prefers warmer ones (at 4.1–8.3 °C) deeper inside and much above the water. G. annulatus overwinters gregariously, profiting in this way from a collective sensing of sudden water rises, while G. titanus overwinters in a solitary manner. Neither Gyas species is cold hardy during overwintering (LT 50 in both about –2 °C), while in post-overwintering specimens, a weak cold hardiness appears (in May LT 50 in both between –3 and –4 °C) which helps them better withstand sudden summer chillings. During overwintering, G. annulatus spends about a quarter of the total energy, but twice as much glycogen as G. titanus ; the captive G. annulatus spent 5.20 J g –1 dry mass day –1 from 0.122 lipids and 0.026 mg g –1 dry mass day –1 glycogen, and G. titanus 21.22 from 0.538 lipids and 0.014 glycogen, respectively. The general energy saving, excessive glycogen supply and overwintering gregariousness in G. annulatus reflect its optimal adaptation to preferred alpine habitats close to water current surfaces in the active ecophase, as well as during lasting overwintering.

Wnt-10b directs hypermorphic development and transformation in mammary glands of male and female mice.

Wnt-10b is expressed during the formation of the mammary rudiment in mouse embryos and its expression continues through puberty when the mammary ductal pattern is established under control of ovarian steroids. Recently, viral activation of the Wnt-10b locus has linked its overexpression to mammary tumor formation, suggesting a role for Wnt-10b in patterning and growth-regulation of the mammary gland. To test this notion, we created lines of transgenic mice that express elevated levels of Wnt-10b under the control of the MMTV promoter/enhancer. Overexpression of this gene resulted in profound developmental alterations in the mammary gland, including expanded glandular development and the precocious appearance of alveoli in virgin females. Moreover, transgenic male mice also exhibited dramatic mammary development involving highly branched mammary ducts and gynecomastia. Aberrant expression of Wnt-10b in the mammary rudiments of males evidently bypasses the normal requirement for ovarian hormonal control in stimulating mammary ductal growth and the repressive effects of androgens. In addition to these developmental effects, transgenic mice of both sexes were highly susceptible to the development of mammary adenocarcinomas. Such tumors arose in a solitary manner indicating that Wnt-10b is a proto-oncogene which provides a necessary, but insufficient signal for oncogenesis. Relevant to this, there was no evidence of amplified expression of FGF mRNAs in these tumors though the Fgf's are a class of genes often implicated as collaborators in Wnt-mediated tumor formation. Indeed, co-expression of MMTV-Wnt-10b and MMTV-FGF-3/int-2 resulted in sterile offspring with highly disorganized mammary epithelium, demonstrating a potent interaction between their respective developmental pathways. These results suggest that Wnt-10b, or other Wnt genes expressed early in mammary development, play a role in regulating sexual dimorphism and show potent transforming activity when overexpressed.

Analysis of 2,3,7,8-TCDD tumor promotion activity and its relationship to cancer

2,3,7,8-tetrachlorodibenzo- p-dioxin (hereafter referred to as TCDD) has a high estimated cancer potency in animals which has been reasoned to imply that TCDD might be carcinogenic to man. The animal cancer data show that TCDD can act in a solitary manner causing tumors without the participation of other known factors. However, there exists animal cancer data indicating that TCDD can act as a tumor promoting compound. This analysis examines which type of carcinogen and which mechanism best characterizes TCDD cancer activity. It is suggested that TCDD acts by a hormonal mechanism to cause cancer in a solitary manner, at low doses, in two species, and in a number of different organs, including rare sites. These observations in toto characterize TCDD as a complete carcinogen, which by definition encompasses both initiation and promotion carcinogenic activities.