Soil In Japan Research Articles

Различные формы Sr в агротемногумусовых подбелах юга Приморского края

To assess the concentration level and identify the main phase-carriers of various forms of strontium (Sr), the total content, distribution and concentration of water-soluble forms of the element in Albic Stagnosoils, which composed the bulk of the arable fund of the region under study, were studied. Full-profile soil pits were dug at the long-term fallow, phytoreclamative, and fertilized with mineral and organic fertilizer variants of the experience. The study of the different Sr forms contents was carried out by energy dispersive X-ray fluorescence spectroscopy and atomic absorption spectrometry. The main Sr volume was immobilized in the composition of soil mineral phase in soils of all variants of the experience. The addition of mineral fertilizers to soil was accompanied by an increase in the total content (by 6.4 %) and concentration of water-soluble (by 5,7 times) Sr forms, compared to the soil of fallow, and intensification of Sr sorption by Mn-containing soil compounds. The long-term addition of different types of organic fertilizers contributed to the activation of stable Sr-organic complexes formation. Under a green manure application in conditions of soil solution acidity decrease, the increase in the influence of Ca-containing soil compounds on the distribution of Sr has been shown. The soils of all variants of the experience were characterized by the absence of factors and conditions that contributed to the Sr accumulation in profiles, it was suggested by the lower total Sr content compared to the mean background content for surface soils around the world and to the mean content in agricultural soils in Japan.

Residence time effects on arsenate sorption on an allophanic Andosol

ABSTRACT The mobility and bioavailability of As in agricultural soils are controlled by sorption onto minerals. The effect of the residence time on the reaction between arsenate and allophanic Andosol, a typical agricultural soil in Japan, remains unclear. Therefore, we investigated the reaction between arsenate and allophanic Andosol and its components allophane and ferrihydrite over several months, based on the desorption experiment and X-ray diffraction (XRD) measurements in the long-term sorption experiment. The reaction between arsenate and three sorbents continued for approximately 150 d. The XRD patterns of allophane and ferrihydrite that reacted with arsenate did not change during the long-term sorption experiments under near-neutral pH conditions, suggesting negligible formation of surface precipitation. Furthermore, the irreversibility of arsenate sorption onto allophanic Andosol and allophane increased with extended residence time. These results suggested that the slow sorption of arsenate onto the materials could be ascribed to internal diffusion.

Effects of soil and phylogeny on sulfur and phosphorus concentrations in wild plants on volcanic and non-volcanic soils in Japan

ABSTRACT Andosols are soils characterized by relatively high sulfur concentrations, but also by their low phosphorus availability for crops and vegetables. We assessed the effect of soil type on the concentrations of sulfur and phosphorus in plants using handheld X-ray fluorescence (XRF) analysis on herbarium specimens collected from Andosols and non-volcanic soils in Japan. The sulfur concentrations in plants from Andosols were higher than plants from non-volcanic soils, but there was no significant difference between their foliar phosphorus concentrations. Only three specimens out of 1203 herbarium specimens were below the sulfur concentration (1000 mg kg−1) needed for adequate growth of crops, but about half of specimens were below that for phosphorus (2000 mg kg−1). The sulfur concentrations were high in herbaceous plants, especially in plants from the Brassicaceae and Liliaceae families. Overall, the results show that the sulfur concentrations in wild plants from Japan were higher in Andosols, with a low risk for sulfur deficiency. In contrast, the effect of soil type on the phosphorus concentrations in plants was small, and this may be explained because most wild plants grow under phosphorus-limiting conditions. Finally, the analysis reveals a strong correlation between sulfur and phosphorus concentrations in plants, suggesting the existence of a mechanism that controls the sulfur and phosphorus concentration ratios.

Biochar from paddy field - A solution to reduce PFAS pollution in the environment

Generally, activated carbons demonstrated a notable ability to capture long-chain PFAS, but exhibited relatively lower effectiveness for short-chain PFAS. Thirteen commercially available activated carbons in Japan underwent testing for their adsorption capacity of PFAS in water. The activated carbon derived from rice husk, Triporous™-PFAS, exhibited the highest adsorption capacity (over 95%) for PFAS from ultrashort-chain (perfluorocarbon chain: C1 for perfluorocarboxylic acid (PFCA) and C2 for perfluoroalkane sulfonic acid (PFSA)) to long-chain PFAS (C13 for PFCA and C10 for PFSA). An earlier lysimeter study highlighted Andosol, representative soil in Japan, as a potential medium for removing PFAS from irrigation water. Considering cultivating rice on Andosol fields and producing biochar from rice husks and rice straw, a new rice cultivation system is proposed. This system aims to facilitate continuous removal of PFAS from the environment in Asia. Japanese rice cultivation system produces not only rice but also biochar to remove PFAS from water circulation system. The total fluorine content in the tested activated carbon materials ranged from 0.18 to 38 μg g−1 F. Based on the results from background F blank and adsorption capacity, TriporousTM-PFAS-F was shown to be an option to lower the method detection limit for a proposed international standard method for measuring total PFAS.

Effect of particle size distribution of sediments on development of polder soils in Japan

Abstract Purpose Polder soils develop from oceanic and lacustrine sediments covered with seawater, brackish water, and freshwater after artificial drainage. Because there are several concerns regarding the agricultural use of polder soils, soil genesis and properties have been considerably surveyed, mainly focusing on problematic soils developed from fine sediments. Although sediments have a wide range of particle size distributions due to different sedimentary conditions, particle size of parent materials have not been well addressed to understand the soil developmental process. In this study, Japanese polders with different reclamation ages and sedimentary conditions were surveyed to clarify the soil formation process and factors affecting pedogenesis. Materials and methods Soil samples were collected from 15 soil profiles in six Japanese polders under different land use types. Sedimentary conditions of polders were evaluated from particle size distributions using the hydrodynamic classification proposed by Pejrup (The triangular diagram used for classification of estuarine sediments: a new approach. Tide-influenced Sediment Environ Facies, pp 289–300, 1988). The major soil-forming factors of polders were extracted by principal component analysis (PCA) using general soil properties. Results and discussion Brackish lake and inner bay polders were characterized by calm hydrodynamic conditions comprising fine particles. Two polders reclaimed from a shallow inland sea were characterized by violent hydrodynamic conditions. Sandy sediments were also characteristic of immature soils reclaimed from a freshwater lake and an estuarine tidal flat. Soils on polders developed under calm hydrodynamic conditions enabled the accumulation of high total carbon content. The soil-forming process in the brackish bay oxidized pyrite, leading to an acidic soil reaction. Conversely, soils developed from sandy sediments were characterized by low iron content. The PCA extracted two factors explained by particle size and soil reaction relating to acidification and salt leaching. Conclusion Polder soils can be mainly discriminated by their particle size distributions, which are characterized by hydrodynamics under the sedimentary conditions, and the polder soil development is affected by water management in land uses after artificial drainage.

Efficient production of polyhydroxybutyrate using lignocellulosic biomass derived from oil palm trunks by the inhibitor-tolerant strain Burkholderia ambifaria E5-3.

Lignocellulosic biomass is a valuable, renewable substrate for the synthesis of polyhydroxybutyrate (PHB), an ecofriendly biopolymer. In this study, bacterial strain E5-3 was isolated from soil in Japan; it was identified as Burkholderia ambifaria strain E5-3 by 16S rRNA gene sequencing. The strain showed optimal growth at 37°C with an initial pH of 9. It demonstrated diverse metabolic ability, processing a broad range of carbon substrates, including xylose, glucose, sucrose, glycerol, cellobiose, and, notably, palm oil. Palm oil induced the highest cellular growth, with a PHB content of 65% wt. The strain exhibited inherent tolerance to potential fermentation inhibitors derived from lignocellulosic hydrolysate, withstanding 3g/L 5-hydroxymethylfurfural and 1.25g/L acetic acid. Employing a fed-batch fermentation strategy with a combination of glucose, xylose, and cellobiose resulted in PHB production 2.7-times that in traditional batch fermentation. The use of oil palm trunk hydrolysate, without inhibitor pretreatment, in a fed-batch fermentation setup led to significant cell growth with a PHB content of 45% wt, equivalent to 10g/L. The physicochemical attributes of xylose-derived PHB produced by strain E5-3 included a molecular weight of 722kDa, a number-average molecular weight of 191kDa, and a polydispersity index of 3.78. The amorphous structure of this PHB displayed a glass transition temperature of 4.59°C, while its crystalline counterpart had a melting point of 171.03°C. This research highlights the potential of lignocellulosic feedstocks, especially oil palm trunk hydrolysate, for PHB production through fed-batch fermentation by B. ambifaria strain E5-3, which has high inhibitor tolerance.

Draft genome sequence of Intrasporangium sp. DVR, an actinobacterium of the family Intrasporangiaceae isolated from soil in Japan.

Intrasporangium sp. strain DVR is an actinobacterium of the family Intrasporangiaceae isolated from soil in Japan. Here we report the draft genome sequence of strain DVR.

Relationship between nitrate, heavy metal, and sterols contents in Japanese agricultural soils with risk of groundwater pollution

In Japanese agricultural lands, nitrate-nitrogen contamination of soil and groundwater often occurs due to the application of livestock excrements and compost. Therefore, rural soils in Japan were sampled and analyzed for nitrate-nitrogen leaching, heavy metal content, and sterols associated with livestock excrement and compost to calculate contamination risk indicators. The results were analyzed using self-organizing maps and cluster analysis. Nitrate-nitrogen content using water extraction was detected in most of the sampled soils. In addition, many samples from areas that were already severely contaminated with nitrate-nitrogen showed particularly high concentrations. Coprostanol, an indicator of fecal contamination, was detected in more than half of the samples. The main source of nitrate-nitrogen contamination in these areas is livestock excrement and compost. Self-organization maps showed that areas with high nitrate-nitrogen contamination also corresponded to areas with high copper and zinc soil contents. The self-organization maps and cluster analysis resulted in five clusters: a nitrate-contaminated group mainly originating from livestock excrement and compost, a heavy metal-contaminated group, a general group, a nitrate-contaminated group mainly originating from chemical fertilizers, and a contaminated group with potentially hazardous substances requiring attention. Authorities and decision-makers can use the results to prioritize areas requiring remediation.

Isolation and habitat analysis of Balamuthia mandrillaris from soil.

Balamuthia mandrillaris is a free-living amoeba that causes meningoencephalitis in mammals. Over 200 cases of infection were reported worldwide, with a fatality rate of over 95%. A clear route of infection was unknown for a long time until a girl died of granulomatous amoebic encephalitis (GAE) in California, USA, in 2003 due to infection with B. mandrillaris detected in a potted plant. Since then, epidemiological studies were conducted worldwide to detect B. mandrillaris in soil and other environmental samples. We previously reported the isolation of B. mandrillaris from the soil in Japan; however, the existing B. mandrillaris culture method with BM3 medium and COS-7 cells was unsuccessful. Therefore, in this study, we aimed to conduct soil analysis to determine the growth conditions of B. mandrillaris. B. mandrillaris-positive soils were defined as soils from which B. mandrillaris was isolated and environmental DNA was PCR-positive. Soils inhabited by B. mandrillaris were alkaline, with high electrical conductivity and characteristics of nutrient-rich soils of loam and clay loam. The results of this study suggest a possible reason for the high prevalence of GAE caused by B. mandrillaris among individuals employed in agriculture-related occupations.

Cycloheximide in the nanomolar range inhibits seed germination of Orobanche minor.

From the 992 samples of culture extracts of microorganisms isolated from soil in Japan, we found that the extract of Streptomyces sp. no. 226 inhibited Orobanche minor seed germination without significantly affecting the seed germination of Trifolium pratense and the growth of Aspergillus oryzae and Escherichia coli. Using ESI-MS, 1H-NMR, and 13C-NMR, we identified the active compound as cycloheximide. Cycloheximide had half-maximum inhibitory concentrations of 2.6 ng/mL for the inhibition of seed germination of O. minor and 2.5 µg/mL for that of the conidial germination of A. oryzae. Since cycloheximide is known to inhibit translation by interacting with ribosomal protein L28 (RPL28) in yeast, we investigated whether RPL protein of O. minor plays a critical role in the inhibition of O. minor seed germination. Our data suggested that O. minor RPL27A was not sensitive to cycloheximide by comparing it to the strain expressing S. cerevisiae RPL28. These findings suggest the presence of an unidentified mechanism by which cycloheximide hinders O. minor seed germination.

Impact of Heat on Soil Water Repellency in Forest Soils from Different Depths using Water-Repellent Japanese Cedar (Cryptomeria japonica) Forest Soil

ome soils under plant species, such as casuarina, pine, eucalyptus, cedar, and cypress, show water- repellent conditions. Wildfires are common in forests dominated by these plant species as they produce highly flammable debris. The heat generated during wildfires alters soil characteristics, including soil water repellency (SWR). The responses of SWR to heat can differ depending on the heating temperature and the soil depth. This study aimed to examine the effects of different heating temperatures on SWR through a soil profile using Japanese cedar (Cryptomeria japonica) forest soil in Japan. Soil samples were collected from four different depths (0-5, 5-10, 10-15, 15-20 cm). Soils were exposed to heat with seven heating temperatures (TH) (50, 100, 150, 200, 250, 300, and 350° C) separately for 1 h using a programmable muffle furnace. The degree (contact angle) and the persistence of SWR in both heated and non-heated samples were measured using the molarity of an ethanol droplet test and the water drop penetration time (WDPT) test, respectively. In non-heated soils, the 0-5 cm layer showed the highest SWR (contact angle ~110°; WDPT≥3600 s). SWR decreased with depth to be non-repellent at 15-20 cm (contact angle≤90°; WDPT≤1 s). In heated samples, SWR of 0-5, 5-10, and 10-15 cm layers decreased with increasing TH, while the selection from 15-20 cm was non-repellent in all treatments. Soils of 0-5 cm depth showed extreme SWR (WDPT=≥3600 s) up to 200° C and became non-repellant at 250° C, while those of 5-10 cm showed extreme SWR up to 150° C, severe SWR (WDPT~1350 s) at 200° C, and became non- repellent at 250° C. The soils from 10-15 cm showed severe SWR (WDPT~2100 s) at TH of 50° C and became non-repellent at 100° C. Results revealed that upper soil layers with higher SWR required higher TH to become non-repellent, and soils from lower layers with lower SWR became non-repellent at lower TH. Further experiments are necessary to identify the changes in molecular levels of organic matter in response to the impacts of heat on SWR. 
 Keywords: Cryptomeria japonica, Laboratory heating, Soil water repellency, Wildfires

Analysis of Rare Earth Elements in Pteridophytes from the Historical Mining Area, Southwestern Japan

Recent studies have shown that various fern species can accumulate REEs in the soil and may be utilized as indicator plants. Athyrium yokoscense, commonly called hebino negoza in Japan, is a pteridophyte that is used as an indicator of metal-contaminated soils in Japan. Mining and mineral processing increase the potential of mine minerals to oxidize and change their chemical form. A study on the biogeochemical behavior of REEs in the mine area needs to be conducted due to the significant use of REEs as an essential material for various applications in the world. The REE content in sediment and pteridophytes under different mining areas was analyzed by inductively coupled plasma-mass spectrometry. The concentration of REEs contained in Athyrium yokoscense was analyzed and then compared to sediment to determine the ability of the plant to absorb and accumulate rare earth elements in the mine area. Sediment and plant samples were collected from three mining areas: Ikuno mine, Akenobe mine, and Tada mine. Athyrium yokoscense plants were collected and digested with hydrogen peroxide (H2O2) and nitric acid (HNO3) at a ratio of 2:5. Sediment samples were taken and digested by the alkali fusion method using lithium tetraborate and then processed using the conventional column method. The present study determined that the concentration of REEs varies in every location, and light REEs tend to be more abundant in sediments than heavy REEs. The amount that can be transferred to ferns also varies. Athyrium yokoscense in the ex-mining area accumulates proportionately more LREEs than HREEs, specifically in the Athyrium yokoscense leaves. The characteristic of the element significantly contributes to the accumulation of REEs in pteridophytes.

Draft genome sequence of Pelosinus sp. IPA-1, a bacterium isolated from arsenic-contaminated soil in Japan.

Pelosinus sp. strain IPA-1 is a bacterium isolated from arsenic-contaminated soil in Japan. We here report the draft genome sequence of strain IPA-1.

Base flow separation for soil erosion simulation in a granitic forested headwater catchment using a process-based model, GeoWEPP

Distributed erosion models, which simulate the physical processes of water flow and soil erosion, are effective for predicting soil erosion in forested catchments. Although subsurface flow through multiple pathways is dominant for runoff generation in forested headwater catchments, the process-based erosion model, Geo-spatial interface for Water Erosion Prediction Project (GeoWEPP), does not have an adequate subsurface component for the simulation of hillslope water flow. In the current study, the quick flow related to soil erosion during rainfall events is separated from the total hydrograph using base flow separation for GeoWEPP to use direct runoff to predict sediment discharge from a granitic-forested catchment in Japan. Water runoff and sediment discharge also are simulated using the conventional method that reproduces the total hydrograph, and the accuracy of the calculation and estimated erodibility parameters are evaluated. The proposed procedure reproduced the quick-flow runoff during rainfall and the cumulative sediment discharge from the catchment. Simultaneously, the proposed procedure overestimates water runoff during extreme weather events, such as typhoons. The estimated erodibility parameters for the hillslope soil were comparable to those of the surface soil in Japan using both the proposed procedure and the conventional one. Conversely, the channel erodibility obtained from the proposed procedure matched the observed sediment characteristics in the granitic forested catchment. The proposed procedure is a promising method to predict sediment discharge from forested catchments based on the geological features of the catchment.

Estimation models from soil pH with a solid-to-liquid ratio of 1:2.5 to pH measured by other methods using soils in Japan

ABSTRACT In Japan, soil pH is primarily measured using water with a solid-to-liquid (SL) ratio of 1:2.5, whereas soil pH measurement methods vary by country. Although some soil pH estimation models for each country have been developed to convert soil pH measured by other methods, suitable models for soils in Japan remain unidentified. Therefore, we propose models for estimating the various soil pH values from those measured by water with an SL ratio of 1:2.5. We collected a dataset of 192 topsoil properties in paddy-upland rotation fields in eastern Japan. The dataset comprised soil pH, electrical conductivity (EC, SL ratio of 1:5), particle density, silt and clay content, and total carbon. Soil pH was measured using distilled water (DW), 0.01 M calcium chloride (CaCl2), and 1 M potassium chloride (KCl) with an SL ratio of 1:1 (pH-1DW), 1:2.5 (pH-2.5DW, pH-2.5CaCl2, pH-2.5KCl), and 1:5 (pH-5DW, pH-5CaCl2, pH-5KCl). The comparison of linear regression models showed that the models comprising pH-2.5DW and intercept were sufficiently accurate to estimate pH-1DW and 5DW. However, to estimate pH-CaCl2 and pH-KCl, the models comprising pH-2.5DW, EC, and intercept were sufficiently accurate. From the study results, we proposed the following equations: pH-1DW = 1.10 × pH-2.5DW − 0.69 (R2 = 0.92, RMSE = 0.19); pH-5DW = 0.80 × pH-2.5DW + 1.32 (R2 = 0.86, RMSE = 0.16); pH-2.5CaCl2 = 0.93 × pH-2.5DW + 1.75 × EC − 0.44 (R2 = 0.94, RMSE = 0.14); pH-5CaCl2 = 0.83 × pH-2.5DW + 2.19 × EC + 0.29 (R2 = 0.88, RMSE = 0.20); pH-2.5KCl = 0.92 × pH-2.5DW + 2.86 × EC − 0.97 (R2 = 0.93, RMSE = 0.15); pH-5KCl = 0.88 × pH-2.5DW + 2.94 × EC − 0.57 (R2 = 0.93, RMSE = 0.15).

The effects of phosphate and pH on arsenate adsorption on allophanic Andosols in Miyazaki

ABSTRACT Arsenic (As) contamination in agricultural soils is a serious problem. The mobility and bioavailability of As in agricultural soil are mainly controlled by phosphate and pH. Although there is a potential risk of soil contamination in Japan by geogenic As distributed along the volcanic front, few studies have investigated arsenate adsorption on Andosols, a typical agricultural soil in Japan. In this study, we evaluated the effects of phosphate and pH on arsenate adsorption on allophanic Andosols. The adsorption isotherms of Andosols for arsenate and phosphate were determined in batch experiments at different phosphate concentrations and pH. In addition, following the competitive adsorption experiment of arsenate and phosphate, we determined three fractions of the adsorbed arsenate by sequential extraction as easily soluble As fraction (F1; 0.01 mol L−1 NaNO3 extraction), specifically adsorbed As and As mainly bound to poorly crystalline materials (F2; 0.2 mol L−1 NH4-oxalate buffer extraction), and As mainly bound to crystalline materials (F3; 0.2 mol L−1 NH4-oxalate buffer+0.1 mol L−1 ascorbic acid extraction). Andosols adsorbed~96.0% arsenate and~98.3% phosphate at the initial arsenate concentration of 214 µmol L−1 and phosphate concentration of 323 µmol L−1, respectively in each single-anion system. The maximum arsenate adsorption on the Andosols occurred at an equilibrium pH of~3.8 and it decreased with further increasing or decreasing equilibrium pH. Upon 300 µmol L−1 of arsenate addition, the Andosols adsorbed 98.6, 99.4, 91.0, and 65.8% of arsenate at an equilibrium pH of 3.0, 3.8, 6.2, and 7.6, respectively. The effect of pH was incorporated into the parameter of the modified Freundlich model for arsenate adsorption on Andosols. The competitive adsorption experiment results indicated a greater affinity of phosphate than arsenate for Andosols. Sequential extraction revealed that competition with phosphate not only decreased the amount of adsorbed arsenate, but also increased the amount of arsenate in F1, which is mobile and readily available to plants. Thus, phosphate fertilization can increase the mobility and bioavailability of arsenate in agricultural soils.

Decoupling of cellulose decomposition and glucose mineralization in volcanic forest soils

ABSTRACT Decomposition of organic matters in volcanic soils (ando soils) is generally slowed by sorption onto short-range-order minerals, while decomposition of non-charged substrates such as cellulose and glucose is hypothesized to be promoted by high microbial biomass and nitrogen availability, irrespective of short-range-order minerals. To analyze factors regulating decomposition activities of cellulose and glucose, we measured the decomposition rates of cellulose filter papers and the mineralization rates of 14C-labeled glucose in five volcanic soils in Japan. Glucose mineralization activities increased with increasing microbial biomass C and N, while cellulose decomposition activities (standardized with cumulative temperature) were not related to microbial biomass C or N. Cellulose decomposition activities increased with increasing ratio of soil available N relative to microbial biomass N (microbial N availability), while they decreased with decreasing soil pH and with increasing fungal activities. Soils with relatively high soil pH and microbial N availability exhibit the relatively high potentials of cellulose decomposition. Because cellulose decomposition, rather than glucose mineralization, is a rate-limiting step, soil pH and microbial N availability, rather than microbial biomass, could primarily regulate decomposition rates of cellulose and glucose in volcanic soils.

Phylogeny and Physiological Diversity of Cold-adapted Anaerobic Bacteria Isolated from Rice Field Soil in Japan.

Cold-adapted or psychrotrophic fermentative anaerobic bacteria were isolated from rice field soil in a temperate area in Japan using anaerobic enrichment cultures incubated at 5°C. Most isolates were obligately anaerobic, spore-forming rods and affiliated with different lineages of the genus Clostridium based on 16S rRNA gene sequences. The growth temperature ranges and physiological properties of three representative clostridial isolates (C5S7, C5S11T, and C5S18) were examined. Strain C5S7 grew at 0°C, but not at 20°C, and was identified as Clostridium estertheticum, a psychrophile isolated from spoiled, vacuum-packed, chilled meat (blown pack spoilage, BPS). Strain C5S7 produced butyrate, n-butanol, and abundant gases (H2 and CO2) as major fermentation products from the carbohydrates utilized. Strain C5S11T, which was recently described as Clostridium gelidum sp. nov., possessed psychrotrophic properties and grew at temperatures between 0 and 25°C. Strain C5S11T was saccharolytic, decomposed polysaccharides, such as inulin, pectin, and xylan, and produced acetate, butyrate, and gases. Strain C5S18 also grew at 0°C and the optimum growth temperature was 15°C. Strain C5S18 did not ferment carbohydrates and grew in a manner that was dependent on proteinaceous substrates. This strain was identified as the psychrotolerant species, Clostridium tagluense, originally isolated from a permafrost sample. Collectively, the present results indicate that psychrotrophic anaerobic bacteria with different physiological properties actively degrade organic matter in rice field soil, even in midwinter, in a cooperative manner using different substrates. Furthermore, different psychrotrophic species of the genus Clostridium with the ability to cause BPS inhabit cultivated soil in Japan.

Health Effects Caused by Soil Fumigant Chloropicrin, Reduction of Exposure to Chloropicrin, and Alternative Technology of Soil Fumigants

Pesticides are essentially toxic to living things and are used openly, so it is necessary to monitor them to prevent their adverse effects. We have studied farmers'exposure to pesticides during soil fumigation operations with chloropicrin, and have noted a danger to the farmers in the form of dyspnea. We examined accidents/symptoms of residents from chloropicrin reported by the Ministry of Agriculture, Forestry, and Fishery from 2010 to 2019. Eighty percent of the cause of these manifestations was the failure to cover fumigated soil with plastic film. Symptoms shown by residents included eye pain (91%), sore throat (35%), and headache (14%). The most common film used for covering fumigated soil in Japan is polyethylene. The agricultural technology centers in Japan have studied the use of gas barrier films, and found it possible to decrease the amount of chloropicrin used to 1/3, and leakage into the atmosphere to less than 1/10. This technology has become popular in the production of sweet potatoes in Tokushima Prefecture. Soil disinfection by solar heat has also been studied in Japan. These studies have shown positive advancements in the fertilization of soil and in the control of microbes. Chloropicrin has caused occupational exposure to farmers and environmental exposure to local residents. It is advisable that the technologies mentioned above become common practice.

Complete Genome Sequence of Herbiconiux sp. Strain L3-i23 Isolated from Soil in Japan.

This study describes the complete genome sequence of Herbiconiux sp. strain L3-i23, acquired from an assembly of long reads and subsequently polished using short reads. The complete genome comprises a 3,139,863-bp chromosome with a GC content of 69.51% and a circular plasmid (39,507 bp).