Soil Carbon Decomposition Research Articles

Direct Evidence for Microbial Regulation of the Temperature Sensitivity of Soil Carbon Decomposition.

Soil physicochemical protection, substrates, and microorganisms are thought to modulate the temperature sensitivity of soil carbon decomposition (Q10), but their regulatory roles have yet to be distinguished because of the confounding effects of concurrent changes of them. Here, we sought to differentiate these effects through microorganism reciprocal transplant and aggregate disruption experiments using soils collected from seven sites along a 5000-km latitudinal transect encompassing a wide range of climatic conditions and from a 4-year laboratory incubation experiment. We found direct microbial regulation of Q10, with a higher Q10 being associated with greater fungal:bacterial ratios. However, no significant direct effects of physicochemical protection and substrate were observed on the variation in Q10 along the latitudinal transect or among different incubation time points. These findings highlight that we should move forward from physicochemical protection and substrate to microbial mechanisms regulating soil carbon decomposition temperature sensitivity to understand and better predict soil carbon-climate feedback.

Nutrient Addition Enhances the Temperature Sensitivity of Soil Carbon Decomposition Across Forest Ecosystems.

Atmospheric nitrogen (N) and phosphorus (P) depositions have been shown to alter nutrient availability in terrestrial ecosystems and thus largely influence soil carbon cycling processes. However, the general pattern of nutrient-induced changes in the temperature response of soil carbon decomposition is unknown. Yet, understanding this pattern is crucial in terms of its effect on soil carbon-climate feedback. Here, we report that N and P additions significantly increase the temperature sensitivity of soil organic carbon decomposition (Q10) by sampling soils from 36 sites across China's forests. We found that N, P, and their co-addition (NP) significantly increased the Q10 by 11.3%, 11.5%, and 23.9%, respectively. The enhancement effect of nutrient addition on Q10 was more evident in soils from warm regions than in those from cold regions. Moreover, we found that nutrient-induced changes in substrate availability and initial substrate and nutrient availability mainly regulated nutrient addition effects. Our findings highlight that N and P deposition enhances the temperature response of soil carbon decomposition, suggesting that N and P deposition should be incorporated into Earth system models to improve the projections of soil carbon feedback to climate change.

Data transformations cause altered edaphic‐climatic controls and reduced predictability on soil carbon decomposition rates

AbstractData transformation of the reference soil organic matter (SOM) decomposition rates (kref), often derived as turnover times or in alternative formats, is commonly used to develop ecological models for projecting the persistence of SOM. However, the effects of reciprocal or logarithmic transformation of kref on model performance and edaphic‐climatic patterns remain uncertain. Here, we convert published kref values into reciprocal or logarithmic formats and establish machine learning models between the transformed kref and edaphic‐climatic predictors. We show that models trained with the transformed kref exhibit a 11.6%−68.4% reduction in performance upon re‐conversion to kref compared to those trained with the original kref. The variable importance analysis identifies distinct key predictors governing the original kref and its transformed counterparts. This suggests that data transformation alters the relative significance of predictors without necessarily improving kref prediction performance. Consequently, our study underscores the importance of directly focusing on the original values rather than alternative representations when dissecting a given variable's patterns and mechanisms in ecological modeling.

Microplastics alter soil carbon cycling: Effects on carbon storage, CO2 and CH4 emission and microbial community

Abstract Microplastics (MPs) are carbon-rich polymers that are ubiquitous in the environment. With the increase of plastic production, microplastic pollution may be exacerbated and result in significant changes in microbial communities and biogeochemical processes such as carbon cycling, eventually impacting greenhouse gas emission and carbon storage in terrestrial ecosystems. However, current research on the effect of MPs on soil carbon cycling is still limited, and there is a lack of systematic review of the scattered information obtained from previous studies. Accordingly, this review provides a systematic overview of the current knowledge on the effects of MPs on soil carbon cycling and gives future research suggestions. Emerging evidence indicates that MPs could affect soil carbon stability and CO2 and CH4 emission by modifying soil physicochemical and microbiological properties; though biodegradable MPs often exhibit a greater effect than nonbiodegradable ones, the specific effects are highly dependent on plastic type, size and concentration. The specific mechanisms of MPs’ impact on soil carbon cycles remain elusive, which are discussed mainly from the perspective of microbial changes, including microbial biomass, microbial community composition, and key enzymes and functional genes associated with carbon metabolism. Further research is needed to elucidate whether MPs have a positive priming effect on soil carbon decomposition and the biotic and abiotic mechanisms involved. This review paper helps researchers gain a clearer picture of how and through which way MPs impact carbon cycling in soil ecosystems.

The Impact and Regulatory Mechanisms of Nitrogen Fertilizer on Soil Carbon Cycling

Modern agriculture must increase production to meet the growing global demand for food. However, excessive use of nitrogen fertilizer has led to a series of environmental issues. The impact of nitrogen fertilizer on soil carbon cycling is of particular concern, as agriculture is a major global carbon reservoir, and soil carbon cycling is crucial for maintaining ecosystem functionality. Previous research indicates that nitrogen fertilizer not only affects soil carbon storage but also alters the structure and function of soil microbial communities, thereby influencing carbon decomposition rates. Nevertheless, the specific mechanisms underlying the effects of nitrogen fertilizer on soil carbon cycling remain elusive. This study aims to comprehensively and systematically analyze the impact of nitrogen fertilizer on soil carbon cycling and elucidate its regulatory mechanisms. Nitrogen fertilizer exerts complex effects on soil, potentially promoting organic carbon accumulation, yet under certain conditions, it may also lead to carbon losses, closely intertwined with its impact on soil microbial communities. Regional and seasonal variations in the response to nitrogen fertilizer highlight the intricate dynamics of soil carbon storage, emphasizing the need for a thorough spatiotemporal investigation. Nitrogen fertilizer also influences soil microbial diversity, resulting in the excessive enrichment of certain microbial populations, disrupting the balance of microbial diversity in the soil. Moderate use of nitrogen fertilizer stimulates soil microbial metabolic activity, whereas excessive use may lead to a decline in metabolic activity. The regulation of soil carbon decomposition rates involves soil enzyme activity and organic matter decomposition pathways, with moderate nitrogen fertilizer application promoting soil carbon cycling, while high concentrations may impede carbon decomposition pathways. In conclusion, nitrogen fertilizer management should be guided by scientific principles to avoid excessive use. Establishing a systematic monitoring system that considers soil nutrients, microbial communities, and other factors is essential for achieving sustainable nitrogen fertilizer use. This approach aims to promote sustainable agriculture, reduce the burden on ecosystems, and enhance soil health and carbon storage.

Effects of microplastics on carbon release and microbial community in mangrove soil systems

Mangrove ecosystems are major carbon sink biomes and also a sink of microplastics (MPs). The final enrichment of MPs in sediments may have a significant impact on the microbial community and carbon turnover in the soil. However, the effects of MP pollution on the mangrove soil microbial communities and carbon release remain unknown. Here, we conducted a manipulative incubation experiment by adding MPs to soil at different soil depths to examine the effect of enriched MPs on soil microorganisms and its function (i.e., decomposition of soil carbon). The results showed that the addition of MPs had no significant effect on the microbial diversity and CO2 cumulative emission in the topsoil but significantly increased CO2 release from the subsoil. The promoting effect of polylactide (PLA) on the release of CO2 from the subsoil was stronger than that of polyethylene (PE) and aging PE. In the subsoil, the activity of soil extracellular enzymes related to N acquisition increased with the MP addition, indicating an increase in microbial N deficiency. The subsoil was more sensitive to MPs because of the exacerbated nitrogen limitation. MP addition reduced the microbial diversity of the subsoil and altered soil microbial interactions. The increasing abundance of some microbial taxa, especially bacteria related to the sulfur cycle, indicated more active electron transfer and organic carbon mineralization in the subsoil. Our findings suggest that MP contamination has potential effects on microbial communities, nutrient cycling, and carbon release in mangrove soils that vary depending on soil depth.

Microbial traits drive soil priming effect in response to nitrogen addition along an alpine forest elevation gradient

Priming effect is a critical process affecting soil organic carbon (SOC) cycle, however, its drivers and patterns responding to nutrient addition are still unclear in alpine forests. Here, we conducted a 28-day incubation experiment based on the collected soils along an elevational gradient (3500–4300 m) on the southeastern Tibetan Plateau with adding carbon and nitrogen sources. The priming effect and microbial traits were analyzed based on 13C-stable glucose and bioinformatics methods. Results revealed that the carbon priming effect (PEC) ranged from 0.45 to 1.63 mg C g−1 SOC along the altitude, which was significantly associated with both soil organic carbon and total nitrogen. The addition of nitrogen inhibited the PEC and showed a positive correlation with the activities of β-1,4-glucosidase, β-1,4-N-acetyl-glucosaminnidase, β-cellobiosidase and β-xylosidase, while microbial community network became more complex and stable in respond to nitrogen addition. Structural equation modeling indicated that microbial communities, especially fungal communities in alpine regions drove PEC in response to nitrogen addition. Soil enzymes were the important intermediaries which drove the mineralization of soil carbon by microorganisms after adding nitrogen. Microorganisms were more sensitive to nitrogen rather than carbon due to the specific climate of alpine regions. Collectively, our works revealed the response pattern of soil carbon decomposition to nutrient addition in alpine ecosystem, clarifying the contribution of soil microorganisms in regulating carbon decomposition and nutrient cycle along high-elevation gradients in the context of global environmental change.

Drought and Waterlogging Stress Regimes in Northern Peatlands Detected Through Satellite Retrieved Solar‐Induced Chlorophyll Fluorescence

AbstractThe water table depth (WTD) in peatlands determines the soil carbon decomposition rate and influences vegetation growth, hence the above‐ground carbon assimilation. Here, we used satellite‐observed Solar‐Induced chlorophyll Fluorescence (SIF) as a proxy of Gross Primary Production (GPP) to investigate water‐related vegetation stress over northern peatlands. A linear model with interaction effects was used to relate short‐ and long‐term anomalies in SIF with WTD anomalies and the absolute WTD. Most locations showed the occurrence of drought and waterlogging stress though regions with exclusively waterlogging or drought stress were also detected. As a spatial median, minimal water‐related vegetation stress was found for a WTD of −0.22 m (short‐term) and −0.20 m (long‐term) (±0.01 m, 95% confidence interval of statistical uncertainty). The stress response observed with SIF is supported by an analysis of in situ GPP data. Our findings provide insight into how changes in WTD of northern peatlands could affect GPP under climate change.

Wheat straw hydrochar induced negative priming effect on carbon decomposition in a coastal soil.

The mechanisms underlying hydrochar-regulated soil organic carbon (SOC) decomposition in the coastal salt-affected soils were first investigated. Straw-derived hydrochar (SHC)-induced C-transformation bacterial modulation and soil aggregation enhancement primarily accounted for negative priming effects. Modification of soil properties (e.g., decreased pH and increased C/N ratios) by straw-derived pyrochar (SPC) was responsible for decreased SOC decomposition.

Litter quality controls tradeoffs in soil carbon decomposition and replenishment in a subtropical forest

Abstract Species‐rich forests can produce litter of varying carbon (C) and nitrogen (N) composition (i.e. quality), which can affect decomposition and play a central role in long‐term soil organic carbon (SOC) accumulation. However, how differences in litter quality affect SOC decomposition and formation remains unclear over the full litter decomposition trajectory. We followed the in situ complete decomposition of added 13C‐labelled high‐ (low C:N) and low‐quality (high C:N) leaf‐litter and its effect on particulate organic matter (POM) and mineral‐associated organic matter (MAOM) fractions over 2 years in a natural subtropical forest. We found that during early stages of decomposition, low‐quality litter inputs decreased SOC via a positive priming effect (i.e. new C inputs favoured decomposition of native SOC), but these SOC losses were offset by SOC gains observed via a negative priming effect during decomposition of high‐quality litter. In contrast, this pattern reversed during late stages of decomposition—SOC losses via a positive priming effect induced by high‐quality litter were offset by SOC gains via a negative priming effect induced by low‐quality litter. Over the full decomposition of litter, both high‐ and low‐quality litter stimulated microbial breakdown of SOC tied to POM, but also replenished more persistent SOC that associated with soil minerals (MAOM). Altogether, we observed that low‐quality litter formed twice as much new SOC as high‐quality litter (24% vs. 12% of added litter‐C). We extend the notion of the priming effect from primarily a negative role promoting losses of native SOC, to a functional role that can replenish persistent SOC. Synthesis. Our measurements raise the possibility that, in species‐rich forests, high‐ and low‐quality litter decomposition play opposite but dynamically complementary roles in renewing POM—both by inducing its decomposition and formation—while exclusively favouring MAOM formation, which can help explain how differences in litter quality favour SOC accumulation and persistence. Global change factors that shift plant community composition may ultimately affect the fate of soil C, as changes in litter quality may force soil transitions from sinks to sources or sources to sinks of atmospheric CO2.

Contrasting geochemical and fungal controls on decomposition of lignin and soil carbon at continental scale

Lignin is an abundant and complex plant polymer that may limit litter decomposition, yet lignin is sometimes a minor constituent of soil organic carbon (SOC). Accounting for diversity in soil characteristics might reconcile this apparent contradiction. Tracking decomposition of a lignin/litter mixture and SOC across different North American mineral soils using lab and field incubations, here we show that cumulative lignin decomposition varies 18-fold among soils and is strongly correlated with bulk litter decomposition, but not SOC decomposition. Climate legacy predicts decomposition in the lab, and impacts of nitrogen availability are minor compared with geochemical and microbial properties. Lignin decomposition increases with some metals and fungal taxa, whereas SOC decomposition decreases with metals and is weakly related with fungi. Decoupling of lignin and SOC decomposition and their contrasting biogeochemical drivers indicate that lignin is not necessarily a bottleneck for SOC decomposition and can explain variable contributions of lignin to SOC among ecosystems.

Global patterns and edaphic-climatic controls of soil carbon decomposition kinetics predicted from incubation experiments

Knowledge about global patterns of the decomposition kinetics of distinct soil organic matter (SOM) pools is crucial to robust estimates of land-atmosphere carbon fluxes under climate change. However, the current Earth system models often adopt globally-consistent reference SOM decomposition rates (kref), ignoring effects from edaphic-climate heterogeneity. Here, we compile a comprehensive set of edaphic-climatic and SOM decomposition data from published incubation experiments and employ machine-learning techniques to develop models capable of predicting the expected sizes and kref of multiple SOM pools (fast, slow, and passive). We show that soil texture dominates the turnover of the fast pools, whereas pH predominantly regulates passive SOM decomposition. This suggests that pH-sensitive bacterial decomposers might have larger effects on stable SOM decomposition than previously believed. Using these predictive models, we provide a 1-km resolution global-scale dataset of the sizes and kref of these SOM pools, which may improve global biogeochemical model parameterization and predictions.

Spatial effects of nitrogen deposition on soil organic carbon stocks in patchy degraded saline-alkaline grassland

Soil organic carbon (SOC) represents the largest carbon sink and plays a critical role in mitigating global warming. Elevated nitrogen (N) deposition has substantial effects on soil carbon sequestration in grassland ecosystems. However, almost no study has examined the effects of N deposition in widely degraded saline-alkaline grasslands. This is a critical knowledge gap because saline-alkaline degradation is one of the key global threats to carbon sequestration services provided by grasslands. Here, we conducted a 3-year N addition experiment in a patchy degraded saline-alkaline grassland to examine the effects of simulated N deposition on SOC stocks, along with spatial effects for five types of saline-alkaline degraded patches (multi-species-mixture patches; Leymus chinensis patches; Puccinellia tenuiflora patches; Chloris virgata patches and Artemisia anethifolia patches). N addition had no overall effect on SOC stocks in the degraded saline-alkaline grassland, but showed pronounced spatial effects depending on the saline-alkaline characteristics of degraded patches. Specifically, SOC decreased in multi-species-mixture patches with low saline-alkaline stress (soil 7 < pH < 8), but no change was observed in other types of degraded patches with high saline-alkaline stress (soil pH > 9). Although N addition promoted plant growth and increased aboveground biomass in all degraded patches, the resulting aboveground carbon input did not further contribute to soil carbon sequestration. Rather than aboveground biomass, soil pH was the critical controlling factor for soil carbon in the saline-alkaline grassland. However, N addition did not alter soil pH in all degraded patches. In multi-species-mixture patches with lightly saline-alkaline soils, N addition reduced species richness and increased microbial C:N ratio, which could accelerate soil carbon decomposition, thereby decreasing SOC stocks. Our findings suggest that increased global N deposition would not restore natural soil carbon in degraded saline-alkaline grassland, and instead, may further deteriorate soil carbon sequestration in lightly degraded patches with high plant diversity and low saline-alkaline stress.

Stronger linkage of diversity-carbon decomposition for rare rather than abundant bacteria in woodland soils

Soil microbial diversity is important for maintaining ecosystem functions. However, the linkage between microbial diversity, especially rare and abundant bacterial diversity, and carbon decomposition remains largely unknown. In this study, we assessed the establishment and maintenance of rare and abundant bacterial α-diversities at the taxonomic and phylogenetic levels and their linkages with soil carbon decomposition separately in four Chinese woodlands. Compared to abundant bacteria, rare bacteria showed higher community diversity, tighter phylogenetic clustering, wider environmental breadth, stronger phylogenetic signals, and higher functional redundancy. The assembly of the abundant bacterial subcommunity was governed by stochastic (59.2%) and deterministic (41.8%) processes, whereas the assembly of the rare bacterial subcommunity was mainly dominated by deterministic processes (85.8%). Furthermore, total phosphorus, soil pH, and ammonium nitrogen balanced stochastic and deterministic processes in both rare and abundant bacterial subcommunities. Our results reveal that rare bacteria displayed stronger environmental adaptability and environmental constraint. Importantly, the α-diversities of rare taxa, rather than abundant taxa, were significantly related to carbon decomposition. This study provides a holistic understanding of biogeographic patterns of abundant and rare bacteria and their α-diversities in relation to carbon decomposition, thus helping us better predict and regulate carbon dynamics under the background of global climate change.

Priming effect on soil carbon decomposition by root exudate surrogates: A meta-analysis

While plant root exudates can greatly and rapidly influence soil organic carbon (C) decomposition via the priming effect, the general pattern of these priming effects remains unexplored. We conducted a meta-analysis with 104 publications to synthesize generalities of the priming effect responses to surrogates of root exudates such as simple sugars, low-molecular-weight organic acids, amino acids and phenolics, and explore their regulators. We found that in general addition of root exudate surrogates significantly stimulated microbial biomass C, enzyme activities, and soil C decomposition by on average 27%, 28% and 39%, respectively, i.e., a positive priming effect. We found distinct differences in the magnitude and regulators of the priming effects caused by root exudate surrogates. Amino acids induced the highest priming effect followed by simple sugars, low-molecular-weight organic acids and phenolics, likely because amino acids provided a source of nitrogen (N) that may have aided the priming effect through favoring microbial growth. The priming effect caused by simple sugars increased but then decreased with soil C to N ratio increasing, and the threshold was at a soil C to N ratio of ∼14. Another important regulator was the incubation temperature, whereas the low-molecular-weight organic acid-induced priming effect was positively correlated with the addition rate. These results emphasize the importance of the specificity of root exudate types with multiple mechanisms causing the priming effect. Future studies should conduct long-term experiments with continuous addition of mixed compounds to provide insights into how changes in root exudates modulate soil C decomposition via the priming effect.

The direct and legacy effects of drying‐rewetting cycles on active and relatively resistant soil carbon decomposition

AbstractGlobal climate change is expected to increase the frequency of drought and heavy precipitation, which could create more frequent drying‐rewetting cycles (DWC) in the soils. Although the DWC effects on soil organic carbon (SOC) decomposition have been widely studied, the effect of DWC and the subsequent legacy effect on the decomposition of different SOC pools is still unclear. We conducted a 128‐d laboratory incubation to investigate the DWC effects by using soils from old‐field for 15 years (OF, representing active SOC), bare‐fallow (BF)for 15 years, and BF for 23 years plus an extra 815‐d incubation (BF+, representing relatively resistant SOC). The experiment included nine 10‐d DWC of three treatments: (1) one mean constant‐moisture at 60% water‐holding capacity (WHC), (2) a mild DWC with 10‐d drying to 40% WHC and immediately rewetting to 80% WHC, and (3) a strong DWC with 10‐d drying to 20% WHC and immediately rewetting to 100% WHC. Following the DWC period (0–90 d), there was a 10‐d stabilization period (adjusting all treatments to 60% WHC), and then a 28‐d extended incubation under the constant moisture of 60% WHC. During the DWC period, the strong DWC had a strong effect on CO2 release compared with the constant‐moisture control, reducing the SOC decomposition of OF by 8% and BF by 10%, while increasing the SOC decomposition of BF+ by 16%. In addition, during the extended period, both mild and strong DWC significantly increased SOC mineralization of OF, but decreased that of BF and BF+. This legacy effect induced by DWC compensated for the changes in CO2 release during the DWC period, resulting in the minor response of SOC decomposition of OF and BF+ to the DWC during the entire incubation. Together, DWC could create both direct and legacy effects, and these effects vary with DWC intensity and SOC pools.

The carbon-quality temperature hypothesis: Fact or artefact?

Climate warming can reduce global soil carbon stocks by enhancing microbial decomposition. However, the magnitude of this loss remains uncertain because the temperature sensitivity of the decomposition of the major fraction of soil carbon, namely resistant carbon, is not fully known. It is now believed that the resistance of soil carbon mostly depends on microbial accessibility of soil carbon with physical protection being the primary control of the decomposition of protected carbon, which is insensitive to temperature changes. However, it is still unclear whether the temperature sensitivity of the decomposition of unprotected carbon, for example, carbon that is not protected by the soil mineral matrix, may depend on the chemical recalcitrance of carbon compounds. In particular, the carbon-quality temperature (CQT) hypothesis asserts that recalcitrant low-quality carbon is more temperature-sensitive to decomposition than labile high-quality carbon. If the hypothesis is correct, climate warming could amplify the loss of unprotected, but chemically recalcitrant, carbon and the resultant CO2 release from soils to the atmosphere. Previous research has supported this hypothesis based on reported negative relationships between temperature sensitivity and carbon quality, defined as the decomposition rate at a reference temperature. Here we show that negative relationships can arise simply from the arbitrary choice of reference temperature, inherently invalidating those tests. To avoid this artefact, we defined the carbon quality of different compounds as their uncatalysed reaction rates in the absence of enzymes. Taking the uncatalysed rate as the carbon quality index, we found that the CQT hypothesis is not supported for enzyme-catalysed reactions, which showed no relationship between carbon quality and temperature sensitivity. The lack of correlation in enzyme-catalysed reactions implies similar temperature sensitivity for microbial decomposition of soil carbon, regardless of its quality, thereby allaying concerns of acceleration of warming-induced decomposition of recalcitrant carbon.

Warming causes variability in SOM decomposition in N‐ and P‐fertiliser‐treated soil in a subtropical coniferous forest

AbstractBoth temperature and nutrient availability have essential roles in regulating the decomposition of soil carbon (C) and nitrogen (N), the main controls on organic matter accumulation in forest ecosystems. However, there is a lack of information about how N deposition and phosphorus (P) additions might impact soil C and N decomposition rates in subtropical forests under climate warming. We measured soil organic C and N mineralisation rates and corresponding exoenzyme activities in a subtropical forest soil that had received N and/or P additions for six years in experimental conditions at a range of temperatures between 10 and 40°C. Our results showed that soil organic C and N decomposition rates were positively correlated with the activities of their corresponding enzymes, which suggests that the extracellular enzyme activities could be the main influence on soil organic matter (SOM) decomposition rates. N additions had a significant positive effect on soil organic C mineralisation and enhanced oxidase and hydrolase activities. P additions had little effect on soil organic C and N decomposition rates. These results challenge the assumptions that soil microorganisms are N‐rich and P availability restricts organic matter decomposition, and provides additional evidence that N, not P, regulates organic matter decomposition in subtropical forests. While N additions significantly influenced the soil C and N decomposition rates, they had little effect on their sensitivity to temperature. In contrast, P additions had a significant effect on the temperature sensitivities of SOM decomposition and the βG and NAG Vmax. Overall, our results show that SOM decomposition is vulnerable to both N and P additions, and both should be considered when predicting how SOM decomposition and C cycling might change under warming.Highlights The effect of N and/or P additions on the temperature sensitivity of SOM decomposition was explored. N stimulated soil organic C decomposition rates and corresponding enzyme activities. P promoted the sensitivity of SOM decomposition to temperature changes. N and P additions could affect the stability of SOM in subtropical forests in the future.

Re-visiting soil carbon and nitrogen stocks in a temperate heathland seven years after the termination of free air CO2 enrichment (FACE)

The response of soil carbon to global climate change remains one of the largest uncertainties for future climate projection. In this study, we re-sampled the soil in a long-term, field-scale, multi-factorial climate experiment, CLIMAITE (Free Air CO2Enrichment (FACE), warming and drought in all combinations in a Danish heathland ecosystem) in 2020, seven years after the experiment was terminated. We aimed to study the dynamics of the soil carbon after the cessation of long-term multi-factorial climate manipulation, with special attention to the fate of the additional soil carbon (19% increase) that was sequestered in plots exposed to elevated CO2 concentrations (eCO2). Soil carbon pools in former eCO2 plots, as well as in drought and warming plots, had normalized again by 2020. However, the difference in soil isotopic composition between ambient and former eCO2 plots remained, indicating similar loss fractions from older and newer soil carbon pools in the eCO2 plots as well as stimulation of the decomposition of old soil carbon via priming. Throughout the study period, soil nitrogen dynamics tracked the changes in soil carbon, suggesting that nitrogen from deeper soil layers was transported upwards to meet increasing plant demand during eCO2 but was lost again from the topsoil after termination of the FACE treatment. Our findings show that the soil carbon and nitrogen pools in this ecosystem are highly dynamic and may respond strongly and rapidly to changes in major ecosystem drivers, and that revisiting climate experiments after the cessation of treatments may provide valuable insights into the dynamics, stability and resilience of major element pools in ecosystems.

Optimizing duration of incubation experiments for understanding soil carbon decomposition

Laboratory incubation is a commonly used method to measure the decomposition of soil organic carbon (SOC). While incubation experiments are conducted across a wide range of durations that may vary from hours to years, no method is available to determine an optimal duration of the incubation experiment so that SOC decomposition can be best understood. Here we presented a novel approach to determine the optimal duration called OPtimal Incubation Duration (OPID). The OPID approach quantifies information gained from an ongoing incubation experiment and determines the time point when SOC decomposition rates can be well quantified. Statistically, the OPID approach is based on a progressive data assimilation algorithm that iteratively assimilates data from an ongoing incubation experiment into a three-pool first-order SOC decomposition model. Using a published incubation data set under different temperatures as a case study, we first generated synthetic daily data, and then fed the data into the three-pool model iteratively to observe the changes of model performance. We found that the accuracy of model projections increased with incubation period and exhibited a trade-off between initial model performance and the time towards accurate projection among different temperatures of incubation. The optimal incubation duration was 347, 212, and 126 days under incubation temperatures of 15 °C, 25 °C and 35 °C, respectively. Comparing the parameters with which from the synthetic daily data, if the incubation period was shorter than the optimal durations, then the decomposition rate of the fast-turnover pool was underestimated and those of the slow pools were overestimated. Sensitivity analysis indicated that optimal incubation duration was negatively correlated with proportion of slow-turnover carbon pools, turnover rates, and initial carbon content, respectively. Our study suggested that long-term incubation experiments are necessary for capturing the dynamics of slow-turnover carbon pools. However, the additional data may not be helpful for model performance if the incubation duration is longer than the optimum. Our study provides a tool for soil scientists to design more effective incubation experiments.DA: data assimilation; PDA: progressive data assimilation; Cum CO2: cumulative CO2 emission from soil; opt: optimal duration;