Sodium Bicarbonate Treatment Research Articles

THE EFFECT OF DİFFERENT PROCESSİNG METHODS ON AMYGDALİN LEVELS AND SOME PHYSİCOCHEMİCAL PROPERTİES OF APRİCOT KERNELS

Apricot seeds, particularly bitter seeds, pose health risks due to their cyanogenic glycosides content. Therefore, determining the amount of amygdalin in seeds is important for the food industry. In this study, the amygdalin content of sweet apricot varieties Hacıhaliloğlu and Kabaaşı, as well as bitter apricot (zerdali), was determined using LS-MS/MS devices. Methods to reduce the amygdalin levels in raw apricot seeds to safe consumption limits were established. The treatments to remove bitterness included sodium bicarbonate alkalinization (4-10% NaHCO3 for 7-15 min), ultrasonic waves (30-50°C for 30-60 min), microwave (240W-560W for 2-5 min), and roasting (120-160°C for 10-20 min). After all treatments, samples were analyzed for fat content, protein, amygdalin content via HCN, antioxidant activity, phenolic compounds, and color values (L, a*, b*, Chroma, Hue) using Soxhlet extraction. The fat content (42.99, 44.35, 40.75 ), protein content (34.14, 30.36, 29.27), and hydrocyanic acid content (mg/kg) (24.56, 30.31, 1630.66) of Hacıhaliloğlu, Kabaaşı, and zerdali varieties were found, respectively. Among the methods used to reduce or eliminate the amygdalin content of raw apricot seeds, roasting emerged as the most effective. For raw zerdali seeds, the amygdalin content was 27.64 mg/g, which decreased to 9.77 after 10 min of roasting at 160°C. In terms of total phenolic content, sweet and bitter seeds had similar values. The antioxidant activity (mg TE/100g) was 82.59 for raw zerdali seeds, with the lowest being for sodium bicarbonate (81.72), ultrasonic (57.5), microwave (52.65), and roasting (97.04) treatments.

Mechanical, free vibration, electrical, and water absorption properties of vinyl ester composites reinforced with Phoenix sp. fibers: Influence of eco‐friendly sodium bicarbonate treatment

AbstractEnvironmentally sustainable and eco‐friendly natural fiber‐reinforced polymer composites have become the materials of interest in replacing synthetic fibers. However, weak interfacial interaction between hydrophilic natural fibers and hydrophobic polymer matrix limits their commercial applicability. Improving the interfacial interaction using environmentally friendly chemical treatments would be beneficial for both industries and the environment. In this study, vinyl ester‐based composites were made by incorporating Phoenix sp. fibers in different content (5, 10, 15, 20, and 25 wt%) and lengths (5, 10, 15, and 20 mm). To improve the interfacial interactions, the fibers were treated with eco‐friendly sodium bicarbonate solution at different durations (24, 120, and 240 h) prior to reinforcing. The fabricated composites were characterized by mechanical, free vibration, electrical resistance, and water uptake properties. The results reveal that both fiber content and fiber length have a significant effect on the above properties. Specifically, the composites incorporated with 20 wt% of 15 mm length fibers offered better properties. Further, the composites added with 120 h treated fibers have the maximum tensile strength (61.35 MPa) and modulus (4.13 GPa), flexural strength (152.63 MPa) and modulus (10.24 GPa), impact strength (24.67 kJ/m2), and natural frequency (56.72 Hz). This improvement is mainly due to the improved interfacial bonding, which was evidenced in morphological analysis. Based on the findings, the eco‐friendly treated sustainable Phoenix sp. fibers could be used as a promising reinforcement material for fabricating light weight polymer composites for various industrial applications.Highlights Various properties of Phoenix sp. fiber/epoxy composites were investigated. Interfacial bonding is enhanced through eco‐friendly chemical treatments. Vibration behavior of composites improved due to high stiffness of treated fibers. Composites with treated fibers have excellent electrical and water resistance.

Sodium Bicarbonate Treatment Promotes Hypoxia and Insulin Resistance in Rats

Sodium Bicarbonate Treatment Promotes Hypoxia and Insulin Resistance in Rats

Influence of Green Chemical Treatments on Morphology and Mechanical Properties of Poly(3‐Hydroxybutyrate‐co‐3‐Hydroxyhexanoate)–Agave Americana Fibers Biocomposites

AbstractThis work aims at investigating the effect of two eco‐friendly chemical treatments of Agave Americana fibers (AAFs) on morphology and mechanical properties of poly(3‐hydroxybutyrate‐co‐3‐hydroxyhexanoate) (PHBHHx) biocomposites prepared by melt compounding. Acid citric and sodium bicarbonate treatments are used separately to modify the fiber surface before their incorporation into PHBHHx matrix at 30 wt%. The study reveals through SEM observations that the fracture surface of untreated PHBHHx biocomposite shows a poor fiber‐matrix adhesion with the presence of many microvoids at interface and also some fibers pullout. However, less surface defects with a finer morphology are observed on the treated biocomposite samples resulting in improved tensile and flexural properties compared with neat polymer, being however, more pronounced for those modified with sodium bicarbonate. The study highlights the beneficial effect of the green treatments investigated to enhance the mechanical properties of PHBHHx biocomposites.

Sodium Bicarbonate Treatment and Clinical Outcomes in Chronic Kidney Disease with Metabolic Acidosis: A Meta-Analysis.

In patients with chronic kidney disease (CKD), impaired kidney acid excretion leads to the onset of metabolic acidosis (MA). However, the evidence is not yet conclusive regarding the effects of sodium bicarbonate in treating CKD with MA. Databases with PubMed, Embase, and the Cochrane Library were used to search for randomized controlled trials from the inception until November 11, 2023 to identify randomized controlled trials investigating the effect of sodium bicarbonate in participants with CKD and MA. The primary outcome was the change in estimated glomerular filtration rate (eGFR). Secondary outcomes included hospitalization rates, change in systolic blood pressure (SBP), all-cause mortality, and mid-arm muscle circumference (MAMC). A random-effects model was applied for analysis, and subgroup, sensitivity analyses were also performed. Fourteen RCTs comprising 2,037 patients demonstrated that sodium bicarbonate supplementation significantly improved eGFR (standardized mean difference [SMD]: 0.33, 95% CI: 0.03 to 0.63, P = 0.03). The group receiving sodium bicarbonate had a lower hospitalization rate (odds ratio: 0.37, 95% CI: 0.25 to 0.55, P < 0.001). Higher MAMC was observed with sodium bicarbonate treatment compared with those without (SMD:0.23, 95% CI: 0.08 to 0.38, P = 0.003, I2 < 0.001). However, higher risk of elevated SBP was found with sodium bicarbonate treatment (SMD:0.10, 95% CI: 0.01 to 0.20, P = 0.03). No significant difference in all-cause mortality was noted. In patients with CKD and MA, sodium bicarbonate supplementation may provide potential benefits in preventing the deterioration of kidney function and increasing muscle mass. However, treatment may be associated with higher blood pressure. Due to the risk of bias stemming from the absence of double-blinded designs and inconsistencies in control group definitions across the studies, further research is crucial to verify these findings.

Effect of combined treatment of curcumin and sodium bicarbonate on quality characteristics of refrigerated beef meatballs

In this study, we assessed the impact of curcumin (CC), sodium bicarbonate (SB), and their combination on the quality characteristics of refrigerated beef meatballs, aiming to ascertain the composite preservation effects of the combined use of CC and SB in meat products. Our findings revealed that, compared to the control group, CC, SB, and their combined treatment significantly reduced the levels of thiobarbituric acid reactive substances, total sulfhydryl losses, total survival counts, and total volatile basal nitrogen values of beef meatballs during storage (P < 0.05). Additionally, these treatments effectively delayed the colour and texture degradation of the meatballs and protected the structure of the meatball myofibrillar proteins. SB exhibited a significant reduction in cooking loss (P < 0.05) by enhancing the pH of beef meatballs and inducing the conversion of α-helix to β-sheet structure in myofibrillar proteins. Furthermore, beef meatballs treated with CC, SB, and their combination displayed dense microstructure and superior sensory quality through storage. The combined treatment of CC and SB yielded the most considerable improvement in the quality of refrigerated beef meatballs. Therefore, CC and SB can be used in combination in meat products to mitigate quality deterioration during storage.

Using ultrasonic-assisted sodium bicarbonate treatment to improve the gel and rheological properties of reduced-salt pork myofibrillar protein

To study the impact of ultrasonic duration (0, 30, and 60 min) and sodium bicarbonate concentration (0% and 0.2%) on the gel properties of reduced-salt pork myofibrillar protein, the changes in cooking yield, colour, water retention, texture properties, and dynamic rheology were investigated. The findings revealed that added sodium bicarbonate significantly increased (P < 0.05) cooking yield, hardness, springiness, and strength of myofibrillar protein while reducing centrifugal loss. Furthermore, the incorporation of sodium bicarbonate led to a significant decrease in L⁎, a⁎, b⁎, and white values of cooked myofibrillar protein; these effects were further amplified with increasing ultrasonic duration (P < 0.05). Additionally, storage modulus (G') significantly increased for myofibrillar protein treated with ultrasonic-assisted sodium bicarbonate treatment resulting in a more compact gel structure post-cooking. In summary, the results demonstrated that ultrasonic-assisted sodium bicarbonate treatment could enhance the tightness of reduced-salt myofibrillar protein gel structure while improving the water retention and texture properties.

Acidosis-mediated increase in IFN-γ-induced PD-L1 expression on cancer cells as an immune escape mechanism in solid tumors

Immune checkpoint inhibitors have revolutionized cancer therapy, yet the efficacy of these treatments is often limited by the heterogeneous and hypoxic tumor microenvironment (TME) of solid tumors. In the TME, programmed death-ligand 1 (PD-L1) expression on cancer cells is mainly regulated by Interferon-gamma (IFN-γ), which induces T cell exhaustion and enables tumor immune evasion. In this study, we demonstrate that acidosis, a common characteristic of solid tumors, significantly increases IFN-γ-induced PD-L1 expression on aggressive cancer cells, thus promoting immune escape. Using preclinical models, we found that acidosis enhances the genomic expression and phosphorylation of signal transducer and activator of transcription 1 (STAT1), and the translation of STAT1 mRNA by eukaryotic initiation factor 4F (elF4F), resulting in an increased PD-L1 expression. We observed this effect in murine and human anti-PD-L1-responsive tumor cell lines, but not in anti-PD-L1-nonresponsive tumor cell lines. In vivo studies fully validated our in vitro findings and revealed that neutralizing the acidic extracellular tumor pH by sodium bicarbonate treatment suppresses IFN-γ-induced PD-L1 expression and promotes immune cell infiltration in responsive tumors and thus reduces tumor growth. However, this effect was not observed in anti-PD-L1-nonresponsive tumors. In vivo experiments in tumor-bearing IFN-γ−/− mice validated the dependency on immune cell-derived IFN-γ for acidosis-mediated cancer cell PD-L1 induction and tumor immune escape. Thus, acidosis and IFN-γ-induced elevation of PD-L1 expression on cancer cells represent a previously unknown immune escape mechanism that may serve as a novel biomarker for anti-PD-L1/PD-1 treatment response. These findings have important implications for the development of new strategies to enhance the efficacy of immunotherapy in cancer patients.

Adverse Impact of Sodium Bicarbonate Administration on Multiple Outcomes in Acute Pancreatitis Patients With Hyperlactatemia.

Hyperlactatemia is likely to occur among patients with acute pancreatitis (AP). Sodium bicarbonate (SB) therapy could be applied to correct potential detrimental acidic disturbances, but the exact impact of SB treatment is unknown. This study aims to investigate the impact of SB on AP patients complicated with hyperlactatemia. The study was conducted based on the database named Medical Information Mart for Intensive Care-IV (MIMIC-IV). Propensity matching (PSM) and inverse probability weighting (IPTW) were used to balance the baseline differences. Multivariate regression and marginal structural Cox models were performed to investigate the association between SB and multiple outcomes. Three hundred fifty-three AP patients with hyperlactatemia (initial serum lactate, >2.0 mmol/L) were extracted from the MIMIC-IV database. We found that SB treatment was significantly associated with worse multi-outcomes of AP patients with hyperlactatemia (in-hospital mortality: hazard ratio, 2.46; 95% confidence interval, 1.38-4.39; P < 0.01). Further analysis through marginal structural Cox models showed that SB had adverse impact on in-hospital prognosis of patients with severe lactic acidosis (pH < 7.15,lactate > 2.0 mmol/L). Sodium bicarbonate might not be an appropriate treatment for AP patients with hyperlactatemia (lactate > 2.0 mmol/L) or with severe lactic acidosis (pH < 7.15, lactate > 2.0 mmol/L).

Effect of Sodium Bicarbonate Treatment on Cognitive Function in CKD

Effect of Sodium Bicarbonate Treatment on Cognitive Function in CKD

The Inhibitory Effect of Sodium Bicarbonate, Phylex and Seaweed Extract Against Penicillium digitatum Causing Green Rot Disease in Local Lemon Fruits

This study was conducted in the College of Agricultural Engineering Sciences/University of Baghdad during the years 2021 and 2022. The study aimed to evaluate the biological activity of Sodium bicarbonate, Phylex and the Seaweed extract in controlling the green mold infection in the local lemon at refrigerated storage conditions (4°C). Two isolates of Penicillium digitatum were identified on Potato Dextrose Agar (PDA) and coded as L1 and L2. The results of this study confirmed the high pathogenicity of P. digitatum isolates to lemon fruits. The symptoms of green rot infection appeared on the fruits after 4 days of pathogen inoculation. The preservative and anti-fungal activity of Sodium bicarbonate, Phylex and Seaweed extract were assessed using local lemon fruits under refrigerated-storage conditions. The highest inhibitory effect was reported when 64 g/L Sodium bicarbonate, 4ml/L Phylex and 128 g/L Seaweed extract were used, preventing 100% of P. digitatum colonies growth after 6 days of treatment at 25±2°C. The data of the refrigerated-storage experiments showed that the Phylex treatment of artificial-wound fruits inoculated with the pathogen was more efficient than Sodium bicarbonate in protecting the lemon fruits from green mold infection. Phylex treatment protected the fruits from the fungal infection for 45 days storage, compared to the Sodium bicarbonate treatment, with which, the fruit infection appeared after 30 days of refrigerated-storage (at 4°C).

The practices of intravenous sodium bicarbonate therapy in neonatal intensive care units: A multi-country survey.

A common occurrence in the neonatal intensive care unit (NICU) is metabolic acidosis. Sodium bicarbonate (SB) has been widely used, but there is insufficient evidence on how SB affects neonates in NICUs with metabolic acidosis. The worsening of intracellular acidosis, the impairment of myocardial function, fluctuations in cerebral blood flow, and intracranial hemorrhage are some of the unfavorable effects of SB treatment in neonates that have been documented in the literature. This study aimed to explore neonatologists' practices for using intravenous SB (ISB) in NICUs. A multi-country survey was carried out in 2022 using an online questionnaire sent to neonatologists in various countries in order to gather information about the use of ISB in NICUs. A previously validated questionnaire was adapted and used in this study. The response rate was 67%. The findings show that 91.2% of neonatologists were using SB to correct metabolic acidosis in the NICU; 71.4% did not have written guidelines for using sodium bicarbonate. The majority of them (78.9%) reported that dosage is included in their guidelines for the use of ISB. The findings of this study emphasize the critical importance of providing guidelines in using ISB for managing metabolic acidosis in NICU to standardize procedures and reduce the use of potentially unsuitable and unsafe treatments, as it has been shown that 71.4% of neonatologists worldwide use sodium bicarbonate without guidelines.

Comparative Evaluation of the Repair Bond Strength of Dental Resin Composite after Sodium Bicarbonate or Aluminum Oxide Air-Abrasion.

The dental prophylactic cleaning of a damaged resin-based composite (RBC) restoration with sodium bicarbonate can change the surface characteristics and influence the repair bond strength. The purpose of this study was to compare the effect of sodium bicarbonate (SB) and aluminum oxide (AO) surface treatments on the microtensile bond strength (µTBS) of repaired, aged RBC. Bar specimens were prepared from microhybrid RBC and aged in deionized water for 8 weeks. Different surface treatments (AO air-abrasion; SB air-polishing), as well as cleaning (phosphoric acid, PA; ethylene-diamine-tetraacetic-acid, EDTA) and adhesive applications (single bottle etch-and-rinse, ER; universal adhesive, UA), were used prior to the application of the repair RBC. Not aged and aged but not surface treated RBCs were used as positive and negative controls, respectively. The repaired blocks were cut into sticks using a precision grinding machine. The specimens were tested for tensile fracture and the µTBS values were calculated. Surface characteristics were assessed using scanning electron microscopy. AO-PA-UA (62.6 MPa) showed a 20% increase in µTBS compared to the NC (50.2 MPa), which proved to be the most significant. This was followed by SB-EDTA-UA (58.9 MPa) with an increase of 15%. In addition to AO-PA-UA, SB-EDTA-UA could also be a viable alternative in the RBC repair protocol.

Sodium bicarbonate for kidney transplant recipients with metabolic acidosis in Switzerland: a multicentre, randomised, single-blind, placebo-controlled, phase 3 trial

Metabolic acidosis is common in kidney transplant recipients and is associated with declining graft function. Sodium bicarbonate treatment effectively corrects metabolic acidosis, but no prospective studies have examined its effect on graft function. Therefore, we aimed to test whether sodium bicarbonate treatment would preserve graft function and slow the progression of estimated glomerular filtration rate (GFR) decline in kidney transplant recipients. The Preserve-Transplant Study was a multicentre, randomised, single-blind, placebo-controlled, phase 3 trial at three University Hospitals in Switzerland (Zurich, Bern, and Geneva), which recruited adult (aged ≥18 years) male and female long-term kidney transplant recipients if they had undergone transplantation more than 1 year ago. Key inclusion criteria were an estimated GFR between 15 mL/min per 1·73 m2 and 89 mL/min per 1·73 m2, stable allograft function in the last 6 months before study inclusion (<15% change in serum creatinine), and a serum bicarbonate of 22 mmol/L or less. We randomly assigned patients (1:1) to either oral sodium bicarbonate 1·5-4·5 g per day or matching placebo using web-based data management software. Randomisation was stratified by study centre and gender using a permuted block design to guarantee balanced allocation. We did multi-block randomisation with variable block sizes of two and four. Treatment duration was 2 years. Acid-resistant soft gelatine capsules of 500 mg sodium bicarbonate or matching 500 mg placebo capsules were given at an initial dose of 500 mg (if bodyweight was <70 kg) or 1000 mg (if bodyweight was ≥70 kg) three times daily. The primary endpoint was the estimated GFR slope over the 24-month treatment phase. The primary efficacy analyses were applied to a modified intention-to-treat population that comprised all randomly assigned participants who had a baseline visit. The safety population comprised all participants who received at least one dose of study drug. The trial is registered with ClinicalTrials.gov, NCT03102996. Between June 12, 2017, and July 10, 2019, 1114 kidney transplant recipients with metabolic acidosis were assessed for trial eligibility. 872 patients were excluded and 242 were randomly assigned to the study groups (122 [50%] to the placebo group and 120 [50%] to the sodium bicarbonate group). After secondary exclusion of two patients, 240 patients were included in the intention-to-treat analysis. The calculated yearly estimated GFR slopes over the 2-year treatment period were a median -0·722 mL/min per 1·73 m2 (IQR -4·081 to 1·440) and mean -1·862 mL/min per 1·73 m2 (SD 6·344) per year in the placebo group versus median -1·413 mL/min per 1·73 m2 (IQR -4·503 to 1·139) and mean -1·830 mL/min per 1·73 m2 (SD 6·233) per year in the sodium bicarbonate group (Wilcoxon rank sum test p=0·51; Welch t-test p=0·97). The mean difference was 0·032 mL/min per 1·73 m2 per year (95% CI -1·644 to 1·707). There were no significant differences in estimated GFR slopes in a subgroup analysis and a sensitivity analysis confirmed the primary analysis. Although the estimated GFR slope did not show a significant difference between the treatment groups, treatment with sodium bicarbonate effectively corrected metabolic acidosis by increasing serum bicarbonate from 21·3 mmol/L (SD 2·6) to 23·0 mmol/L (2·7) and blood pH from 7·37 (SD 0·06) to 7·39 (0·04) over the 2-year treatment period. Adverse events and serious adverse events were similar in both groups. Three study participants died. In the placebo group, one (1%) patient died from acute respiratory distress syndrome due to SARS-CoV-2 and one (1%) from cardiac arrest after severe dehydration following diarrhoea with hypotension, acute kidney injury, and metabolic acidosis. In the sodium bicarbonate group, one (1%) patient had sudden cardiac death. In adult kidney transplant recipients, correction of metabolic acidosis by treatment with sodium bicarbonate over 2 years did not affect the decline in estimated GFR. Thus, treatment with sodium bicarbonate should not be generally recommended to preserve estimated GFR (a surrogate marker for graft function) in kidney transplant recipients with chronic kidney disease who have metabolic acidosis. Swiss National Science Foundation.

Six pills less: no benefit for bicarbonate supplementation in renal allograft recipients

Metabolic acidosis (defined as serum bicarbonate <22 mmol/L) is frequent in patients with chronic kidney disease and is associated with disease progression. 1 Chapter 3: management of progression and complications of CKD. Kidney Int Suppl (2011). 2013; 3 (No authors listed): 73-90 Summary Full Text Full Text PDF PubMed Scopus (90) Google Scholar Several prospective trials and meta-analyses 2 Hultin S Hood C Campbell KL Toussaint ND Johnson DW Badve SV A systematic review and meta-analysis on effects of bicarbonate therapy on kidney outcomes. Kidney Int Rep. 2020; 6: 695-705 Summary Full Text Full Text PDF PubMed Scopus (8) Google Scholar , 3 Witham MD Band M Chong H et al. Sodium bicarbonate to improve physical function in patients over 60 years with advanced chronic kidney disease: the BiCARB RCT. Health Technol Assess. 2020; 24: 1-90 Crossref PubMed Scopus (8) Google Scholar , 4 Di Iorio BR Bellasi A Raphael KL et al. Treatment of metabolic acidosis with sodium bicarbonate delays progression of chronic kidney disease: the UBI study. J Nephrol. 2019; 32: 989-1001 Crossref PubMed Scopus (70) Google Scholar , 5 Dubey AK Sahoo J Vairappan B Haridasan S Parameswaran S Priyamvada PS Correction of metabolic acidosis improves muscle mass and renal function in chronic kidney disease stages 3 and 4: a randomized controlled trial. Nephrol Dial Transplant. 2020; 35: 121-129 PubMed Google Scholar , 6 Navaneethan SD Shao J Buysse J Bushinsky DA Effects of treatment of metabolic acidosis in CKD: a systematic review and meta-analysis. Clin J Am Soc Nephrol. 2019; 14: 1011-1020 Crossref PubMed Scopus (60) Google Scholar have suggested potential positive effects (of low certainty) of sodium bicarbonate treatment on chronic kidney disease progression, and bicarbonate supplementation is recommended in patients with chronic kidney disease with serum bicarbonate less than 22 mmol/L. 1 Chapter 3: management of progression and complications of CKD. Kidney Int Suppl (2011). 2013; 3 (No authors listed): 73-90 Summary Full Text Full Text PDF PubMed Scopus (90) Google Scholar However, protective mechanisms are not fully understood and it is difficult to determine whether acidosis is a cause or consequence of renal function decline. Sodium bicarbonate for kidney transplant recipients with metabolic acidosis in Switzerland: a multicentre, randomised, single-blind, placebo-controlled, phase 3 trialIn adult kidney transplant recipients, correction of metabolic acidosis by treatment with sodium bicarbonate over 2 years did not affect the decline in estimated GFR. Thus, treatment with sodium bicarbonate should not be generally recommended to preserve estimated GFR (a surrogate marker for graft function) in kidney transplant recipients with chronic kidney disease who have metabolic acidosis. Full-Text PDF

Isolation, identification, biological characteristics, and antifungal efficacy of sodium bicarbonate combined with natamycin on Aspergillus niger from Shengzhou nane (Prunus salicina var. taoxingli) fruit.

The fungi causing fruit rot were isolated from symptomatic Shengzhou nane (Prunus salicina var. taoxingli) fruit and were identified as Aspergillus niger by biological characteristics and molecular analysis of the internal transcribed spacer region (rDNA-ITS) and translation elongation factor-1α (TEF-1α) sequences. Optimal growth conditions for A. niger were 30°C, pH 5.0-6.0, and fructose and peptone as carbon and nitrogen sources. The effects of sodium bicarbonate (SBC), natamycin (NT), and combined treatments on A. niger inhibition were investigated. Treatment with 4.0 g/L sodium bicarbonate (SBC) + 5.0 mg/L natamycin (NT) inhibited mycelial growth and spore germination as completely as 12.0 mg/L SBC or 25.0 mg/L NT. SBC and NT treatments disrupted the structural integrity of cell and mitochondria membranes and decreased enzyme activities involved in the tricarboxylic acid (TCA) cycle, mitochondrial membrane potential (MMP), ATP production in mitochondria, and ergosterol content in the plasma membrane, thus leading to the inhibition of A. niger growth. Moreover, experimental results in vivo showed that the rot lesion diameter and decay rate of Shengzhou nane fruit treated with SBC and NT were significantly reduced compared with the control. The results suggest that the combination treatment of SBC and NT could be an alternative to synthetic fungicides for controlling postharvest Shengzhou nane decay caused by A. niger.

Evaluation and Treatment of Cement Contamination in Water Based Mud

Drilling mud is susceptible to contamination, particularly from cement, yet monitoring its characteristics and implementing control and remediation strategies is critical for its performance in safe and cost-effective drilling operations. This study presents an experimental investigation into the evaluation and treatment of cement contamination in Water Based Mud (WBM). Four mud samples were prepared and three were contaminated with 4g, 8g and 12g of cement. A chemical treatment using 6g of sodium bicarbonate was applied as a remedy for contamination. The Rheological properties, mud pH and mud densities of the contaminated and treated Water Based Mud at temperatures of 35°C, 55°C, 75°C and 95°C were determined. Furthermore, the elemental composition of samples of contaminated and treated Water Based Mud was determined using an X-Ray Fluorescence Spectrometer. From the results obtained the mud density increased with a rise in cement concentration for both contamination and treatment with the exception of 8g cement concentration at 75°C and 95°C and 12g cement concentration at 55°C and 75°C where a decrease was observed in the treatment process. The yield point and 10second gel increased for both contamination and treatment process. In contrast, the 10minute gel decreased for both contamination and treatment process. With some exceptions, increasing cement concentration resulted in a decrease in plastic viscosity, whereas treatment with sodium bicarbonate resulted in an increase with some exceptions. The mud pH for the contamination process increased as cement concentration increased, while treatment only marginally increased it with exception of 12g at 75°C and 95°C where a decrease was observed. Sodium bicarbonate treatment improves stability by decreasing excessive calcium and lime contamination from cement, reducing pollutants and enhancing the pH stability of the drilling mud. Additionally, results from the mud characterization indicates a higher percentage of 17.962% lime (CaO) for cement contamination and lower percentage of 15.710% lime (CaO) for treatment with sodium bicarbonate which agrees with literature for the treatment process.

Monitor Tumor pHe and Response Longitudinally during Treatment Using CEST MRI-Detectable Alginate Microbeads.

Imaging pHe of the tumor microenvironment has paramount importance for characterizing aggressive, invasive tumors, as well as therapeutic responses. Here, a robust approach to image pH changes in the tumor microenvironment longitudinally and during sodium bicarbonate treatment was reported. The pH-sensing microbeads were designed and prepared based on materials approved for clinical use, i.e., alginate microbead-containing computed tomography (CT) contrast-agent (iopamidol)-loaded liposomes (Iop-lipobeads). This Iop-lipobead prepared using a customized microfluidic device generated a CEST contrast of 10.6% at 4.2 ppm at pH 7.0, which was stable for 20 days in vitro. The CEST contrast decreased by 11.8% when the pH decreased from 7.0 to 6.5 in vitro. Optimized Iop-lipobeads next to tumors showed a significant increase of 19.7 ± 6.1% (p < 0.01) in CEST contrast at 4.2 ppm during the first 3 days of treatment and decreased to 15.2 ± 4.8% when treatment stopped. Notably, percentage changes in Iop-lipobeads were higher than that of amide CEST (11.7% and 9.1%) in tumors during and after treatment. These findings demonstrated that the Iop-lipobead could provide an independent and sensitive assessment of the pHe changes for a noninvasive and longitudinal monitoring of the treatment effects using multiple CEST contrast.

Effect of Ultrasound-Assisted Sodium Bicarbonate Treatment on Aggregation and Conformation of Reduced-Salt Pork Myofibrillar Protein.

To study the effects of an ultrasound (0, 30, and 60 min) and sodium bicarbonate (0% and 0.2%) combination on the reduced-salt pork myofibrillar protein, the changes in pH, turbidity, aggregation, and conformation were investigated. After the ultrasound-assisted sodium bicarbonate treatment, the pH increased by 0.80 units, the absolute value of Zeta potential, hydrophobic force, and active sulfhydryl group significantly increased (p &lt; 0.05), and the turbidity and particle size significantly decreased (p &lt; 0.05). Meanwhile, the fluorescence intensity decreased from 894 to 623, and the fluorescence peak showed a significant redshift, which indicated that the ultrasound-assisted sodium bicarbonate treatment exposed the non-polarity of the microenvironment in which the fluorescence emission group was located, leading to the microenvironment and protein structure of myofibrillar tryptophan being changed. Overall, an ultrasound-assisted sodium bicarbonate treatment could significantly improve pork myofibrillar protein solubility and change the protein structure under a reduced-salt environment.

Metabolic acidosis is undertreated and underdiagnosed: a retrospective cohort study.

Guidelines recommend treatment of metabolic acidosis (MA) in patients with chronic kidney disease (CKD), but the diagnosis and treatment rates in real-world settings are unknown. We investigated the frequency of MA treatment and diagnosis in patients with CKD. In this retrospective cohort study, we examined administrative health data from two US databases [Optum's de-identified Integrated Claims+Clinical Electronic Health Record Database (US EMR cohort; 1 January 2007 to 30 June 2019) and Symphony Health Solutions IDV® (US claims cohort; 1 May 2016 to 30 April 2019)] and population-level databases from Manitoba, Canada (1 April 2006 to 31 March 2018). Patients who met laboratory criteria indicative of CKD and chronic MA were included: two consecutive estimated glomerular filtration results <60mL/min/1.73m2 and two serum bicarbonate results 12 to <22mEq/L over 28-365 days. Outcomes included treatment of MA (defined as a prescription for oral sodium bicarbonate) and a diagnosis of MA (defined using administrative records). Outcomes were assessed over a 3-year period (1 year pre-index, 2 years post-index). A total of 96184 patients were included: US EMR, 6179; Manitoba, 3223; US Claims, 86782. Sodium bicarbonate treatment was prescribed for 17.6%, 8.7% and 15.3% of patients, and a diagnosis was found for 44.7%, 20.9% and 20.9% of patients, for the US EMR, Manitoba and US Claims cohorts, respectively. This analysis of 96184 patients with laboratory-confirmed MA from three independent cohorts of patients with CKD and MA highlights an important diagnosis and treatment gap for this disease-modifying complication.