Several studies have provided evidence of a women's better accuracy in interpreting emotional states. Despite this difference is generally ascribed to the primary role of female gender in the affective relation with the offspring, to date, little information is available regarding gender differences in the ability to interpret infant facial expressions. In the present study, we examined the roles of gender and expertise in interpreting infant expression in 34 men and women who differed in their experience with infants. Women showed a significantly higher level of decoding accuracy compared to men. Expertise positively affected facial expressions decoding among women only. Our results suggest that in judging emotional facial expressions of infants, there is an interaction of biological (i.e., gender) and cultural factors that is independent of a woman's socioeconomic status.