Smoke Contamination Research Articles

Wildfire Smoke Exposure During Pregnancy: Consensus-Building to Co-Create a Community-Engaged Study

Relative to other Oregon counties, Klamath County experiences worse air quality due to wildfire smoke, as well as elevated rates of infant mortality and low birthweight. Klamath County Public Health (KCPH) raised concerns that wildfire smoke is a contributor to poor infant health. Thus, we built a multidisciplinary team and designed a community-engaged research (CEnR) project to capture community and individual-level exposure to wildfire smoke contaminants, alongside perinatal health outcomes. Through partnerships, we identified 24 individuals across academic, public health, and community organizations that met five times over three months to develop a study design. We initially used a modified Delphi method, but adjusted our approach to find multidisciplinary areas of agreement across a highly diverse team. Our team used structured meetings, surveys, and iterative feedback to build consensus on a study design. KCPH and our community partners reviewed and approved all proposed activities to ensure community input was integrated. The resultant study, trialed in Klamath County, included the use of environmental, residential, and personal samplers and health surveys with a cohort of pregnant individuals during the wildfire season. We discuss the advantages and challenges of building a multidisciplinary CEnR study in a rural county disproportionately impacted by wildfire smoke and infant mortality.

Contamination of surfaces in children's homes with nicotine and the potent carcinogenic tobacco-specific nitrosamine NNK.

Tobacco smoke exposure (TSE) through secondhand and thirdhand smoke is a modifiable risk factor that contributes to childhood morbidity. Limited research has assessed surface TSE pollution in children's environments as a potential source of thirdhand smoke exposure, and none have examined levels of the tobacco-specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) on surfaces. This study measured surface NNK and nicotine in children's homes and associations with sociodemographics and parent-reported TSE behaviors. We assessed correlations of surface NNK and nicotine with dust NNK, dust nicotine, and child cotinine. Home surface wipe NNK and nicotine data from 84 children who lived with smokers were analyzed. Tobit and simple linear regression analyses were conducted to assess associations of surface NNK and nicotine with child characteristics. Spearman's (ρ) correlations assessed the strength of associations between environmental markers and child cotinine. Nearly half (48.8%) of children's home surfaces had detectable NNK and 100% had detectable nicotine. The respective geometric means (GMs) of surface NNK and nicotine loadings were 14.0 ng/m2 and 16.4 µg/m2. Surface NNK positively correlated with surface nicotine (ρ = 0.54, p < 0.001) and dust NNK (ρ = 0.30, p = 0.020). Surface nicotine positively correlated with dust NNK (ρ = 0.42, p < 0.001) and dust nicotine (ρ = 0.24, p = 0.041). Children with household incomes ≤$15,000 had higher surface NNK levels (GM = 18.7 ng/m2, p = 0.017) compared to children with household incomes >$15,000 (GM = 7.1 ng/m2). Children with no home smoking bans had higher surface NNK (GM = 18.1 ng/m2, p = 0.020) and surface nicotine (GM = 17.7 µg/m2, p = 0.019) levels compared to children with smoking bans (GM = 7.5 ng/m2, 4.8 µg/m2, respectively). Although nicotine on surfaces is an established marker of thirdhand smoke pollution, other thirdhand smoke contaminants have not been measured on surfaces in the homes of children living with smokers. We provide evidence that the potent carcinogenic tobacco-specific nitrosamine NNK was detectable on surfaces in nearly half of children's homes, and nicotine was detectable on all surfaces. Surface NNK was positively correlated with surface nicotine and dust NNK. Detectable surface NNK levels were found in homes with indoor smoking bans, indicating the role of NNK as a persistent thirdhand smoke pollutant accumulating on surfaces as well as in dust.

Impact of Smoke from Wheat, Oat, and Clover Stubble Burning on Cabernet Sauvignon Grapes and Wine

Background and Aims. Stubble burning is an agricultural practice employed by some grain growers to prepare farmland for sowing and/or to control weeds and pests. Grapegrowers and winemakers have questioned whether the resulting smoke can contaminate grapes in nearby vineyards. This study therefore sought to determine the potential for smoke from three different stubble burns to taint grapes and wine. Methods and Results. Excised bunches of mature Cabernet Sauvignon grapes were exposed to smoke from prescribed burning of wheat, oat, and Balansa clover stubble windrows. Environmental sensors monitored the concentration of particulate matter as a measure of smoke density, while chemical and sensory analysis established the extent to which grapes and wine were tainted by smoke. Only grapes that were positioned among the burning wheat windrows or downwind, but in close proximity (∼200 m) to the oat stubble burn, were exposed to sufficient quantities of smoke to result in a detectable concentration of volatile phenols (up to 12 µg/kg), as chemical markers of smoke taint. These grapes yielded wines with two to threefold higher volatile phenol concentrations (up to 18 µg/L) than other wines, including the control wine, and low intensity, but perceptible smoke-related sensory attributes, indicative of low-level smoke taint. Conclusions. Chemical and sensory analyses suggest the risk of smoke taint from stubble burning is low, except where vineyards are immediately downwind and/or prolonged or repeated smoke exposure occurs. However, stubble moisture content and prevailing weather conditions affect smoke density and dispersion, and will therefore affect the potential for smoke contamination by grapes. Significance of the Study. This study will assist colocated grain growers and grape and wine producers to undertake commercial operations, without negatively impacting one another.

Novel digital technologies to assess smoke taint in berries and wines due to bushfires

Due to climate change, the higher incidence and severity of bushfires is a significant challenge for wine producers worldwide as an increase in smoke contamination negatively affects the physicochemical components that contribute to the lower quality of fresh produce and final products (smoke taint in wines). This reduces prices and consumer acceptability, impacting the producers and manufacturers. Current methods available to winemakers for assessing contamination in berries and wine consist of costly laboratory analyses that require skilled personnel and are time-consuming, cost prohibitive, and destructive. Therefore, novel, rapid, cost-effective, and reliable methods using digital technologies such as the use of near-infrared (NIR) spectroscopy, electronic nose (e-nose), and machine learning (ML) have been developed by our research group. Several ML models have been developed for smoke taint detection and quantification in berries and wine from different varieties using NIR absorbance values or e-nose raw data as inputs to predict glycoconjugates, volatile phenols, volatile aromatic compounds, smoke-taint amelioration techniques efficacy, and sensory descriptors, all models with &gt;97% accuracy. These methods and models may be integrated and automated as digital twins to assess smoke contamination in berries and smoke taint in wine from the vineyard for early decision-making.

In Utero Thirdhand Smoke Modulates Platelet Gene Expression in Exposed Mice

It is well known that exposure to smoking during pregnancy has been associated with cardiovascular disease (CVD) in the offspring. It is important to note that direct exposure to smoking is not the only means of tobacco exposure during pregnancy. Indeed, there is a new form, termed thirdhand smoke (THS) that has been gaining attention as of late. THS refers to the residual tobacco smoke contamination that remains after a cigarette is extinguished, and which persists for months. This leads to the accumulation of chemicals over time, which undergo chemical reactions and aging, thereby becoming more toxic. To date, the negative effects of in utero THS on CVD are understudied. Since platelets are a major player in the genesis of CVD, we employed a transcriptomic (mRNA and miRNA), in vitro and in vivo‐based approaches to investigate the effects of in utero THS exposure on platelet gene expression and function, as well as the risk of developing thrombosis in the adult offspring. Thus, C57 female mice were exposed to THS or clean air (control) one week before mating and throughout gestation, using a validated exposure protocol. The exposure was stopped at birth, and the offspring males were used for experimentation once they have reached at 8 ‐ 10 weeks of age. RNA‐Seq next generation sequencing demonstrated distinct changes in the gene‐expression profile of circulating platelets of the in uteroTHS exposed mice. Pathway enrichment analysis revealed differential gene‐expression changes in pathways associated with metabolism, oxidative stress, and platelet activation. Moreover, using miRNA‐seq, we found 35 differentially expressed miRNAs in the in uteroTHS exposed platelets compared with controls. The miRNA‐mRNA integrated interactions analysis showed 107 validated interactions including interesting interaction between downregulated micorRNAs (miR‐101a‐3p, miR‐144‐3p, miR‐16‐1‐3p and miR‐542‐5p) and upregulated Cxcl12 protein coding gene, a known platelet activation cytokine. We next sough to confirm that changes in the platelet gene expression in the in uteroTHS exposed mice are accompanied by changes in platelet function. To this end, separate experiments showed that activation of Akt and ERK, key biochemical markers of platelet activation, is enhanced in the in uteroexposed mice, which was accompanied by an enhanced clot retraction response. Consistent with these findings, our in vivo studies showed that in uteroTHS exposed mice have significantly shortened (104 ± 10.21[sec]) survival in the pulmonary thromboembolism model in comparison to the clean air control (179 ± 20.6 [sec]). Collectively, our results show altered platelet gene expression, enhanced platelet function, and increased risk of thrombosis in mice that were subjected to the in utero THS exposure, relative to the control. These findings support the notion that in uteroTHS exposure is detrimental to human health and should increase public awareness and inform policy for managing exposure to this type of smoke.

Using Collaborative Partnerships to Engage Firefighters in Rural Communities.

The purpose of this study was to utilize community-based participatory research (CBPR) methods to identify behaviors that may increase the exposure rates of firefighters to carcinogens and other occupational hazards. Key informant interviews and focus groups were conducted as part of a larger study that included exposure assessments at fire stations, in fire engines, and in personal vehicles. A purposive sample of five fire chiefs and leaders of the firefighter association was chosen, and these individuals were selected to participate in interviews. Unstructured interviews explored issues related to firefighter exposures and occupational hazards. Three focus groups were conducted over a three-month period. Following the focus groups, survey questions emphasizing the following three aspects of firefighting were developed: exposure to airborne smoke contaminants during fire suppression, accumulated exposure on turnout gear after fire suppression, and exposure to airborne contaminants at the fire departments. The use of community-based participatory research can be very beneficial, especially when conducting research with a group that may be hard to reach or have misgivings regarding researchers. By utilizing this approach in the current study, researchers were able to partner with a community that may be hard to reach and gain buy-in from community leadership.

Effects of Outer Shell Fabric Color, Smoke Contamination, and Washing on Heat Loss through Turnout Suit Systems

Firefighters frequently have to work in direct solar radiant heat. To reduce firefighter heat stress, the influence of turnout garment properties on heat gain from solar radiation must be understood. This research studied the effects of color, texture, washing, and contamination of outer shell fabrics on heat loss through firefighter turnout fabric materials in simulated solar exposures. It showed that solar radiation could be a major factor in heat loss through turnout suits. Solar radiation equivalent to a sunny day completely reversed heat exchange through the turnout fabric systems, converting a heat loss of about 240 W/m2 to a heat gain exceeding 100 W/m2. Solar radiation caused turnout fabric systems to dry out and this decreased the performance of turnout systems that incorporated bi-component moisture barriers. Most significantly, the color of the outer shell had a major influence on lowering turnout heat loss in solar exposures. Composites with a black-dyed outer shell absorbed more solar energy than composites with lighter colored shell materials. Soot and fire-ground contaminants present on turnout outer shell fabrics also reduced heat loss under solar exposure. The findings of this study answered long-standing questions about the importance of turnout fabric color on heat exchange with the environment. The results provide additional motivation for efficient turnout cleaning practices, not only to reduce potentially toxic exposure to smoke contaminants, but to reduce turnout-gear-related heat strain on firefighters.

Emerging investigator series: deposited particles and human lung lining fluid are dynamic, chemically-complex reservoirs leading to thirdhand smoke emissions and exposure

Tobacco combustion products in deposited particles and bodily reservoirs enable persistent thirdhand smoke contamination and transport.

Evaluating the Potential for Smoke from Stubble Burning to Taint Grapes and Wine.

It has been well established that bushfire/wildfire smoke can taint grapes (and therefore wine), depending on the timing and duration of exposure, but the risk of smoke contamination from stubble burning (a practice employed by some grain growers to prepare farmland for sowing) has not yet been established. This study exposed excised bunches of grapes to smoke from combustion of barley straw and pea stubble windrows to investigate the potential for stubble burning to elicit smoke taint. Increased levels of volatile phenols (i.e., chemical markers of smoke taint) were detected in grapes exposed to barley straw smoke (relative to control grapes), with smoke density and the duration of smoke exposure influencing grape volatile phenols. However, the sensory panel did not perceive wine made from grapes exposed to low-density smoke to be tainted, despite the presence of low levels of syringol providing compositional evidence of smoke exposure. During the pea stubble burn, grapes positioned amongst the burning windrows or on the edge of the pea paddock were exposed to smoke for ~15–20 and 30–45 min, respectively, but this only resulted in 1 µg/kg differences in the cresol and/or syringol concentrations of smoke-affected grapes (and 1 µg/L differences for wine), relative to controls. A small, but significant increase in the intensity of smoke aroma and burnt rubber flavor of wine made from the grapes positioned amongst the burning pea stubble windrows provided the only sensory evidence of any smoke taint. As such, had vineyards been located immediately downwind from the pea stubble burn, it is unlikely that there would have been any smoke contamination of unharvested grapes.

Assessment of Volatile Aromatic Compounds in Smoke Tainted Cabernet Sauvignon Wines Using a Low-Cost E-Nose and Machine Learning Modelling.

Wine aroma is an important quality trait in wine, influenced by its volatile compounds. Many factors can affect the composition and levels (concentration) of volatile aromatic compounds, including the water status of grapevines, canopy management, and the effects of climate change, such as increases in ambient temperature and drought. In this study, a low-cost and portable electronic nose (e-nose) was used to assess wines produced from grapevines exposed to different levels of smoke contamination. Readings from the e-nose were then used as inputs to develop two machine learning models based on artificial neural networks. Results showed that regression Model 1 displayed high accuracy in predicting the levels of volatile aromatic compounds in wine (R = 0.99). On the other hand, Model 2 also had high accuracy in predicting smoke aroma intensity from sensory evaluation (R = 0.97). Descriptive sensory analysis showed high levels of smoke taint aromas in the high-density smoke-exposed wine sample (HS), followed by the high-density smoke exposure with in-canopy misting treatment (HSM). Principal component analysis further showed that the HS treatment was associated with smoke aroma intensity, while results from the matrix showed significant negative correlations (p < 0.05) were observed between ammonia gas (sensor MQ137) and the volatile aromatic compounds octanoic acid, ethyl ester (r = −0.93), decanoic acid, ethyl ester (r = −0.94), and octanoic acid, 3-methylbutyl ester (r = −0.89). The two models developed in this study may offer winemakers a rapid, cost-effective, and non-destructive tool for assessing levels of volatile aromatic compounds and the aroma qualities of wine for decision making.

Influence of ink and smoke ATM security systems on dactyloscopy and subsequent DNA analysis after detonation

Automated Teller Machine bombings are an increasing societal problem that are often committed using Improvised Explosive Devices. The evolution in IEDs and the negative consequences for society require new security measures to prevent these crimes. Ink staining and security smoke devices are added to cash cassettes, in order to protect ATMs and prevent ATM bombings. Traces found at crime scenes, such as fingerprints and DNA, can contribute to the identification of perpetrators. However, the effect of ink staining and security smoke devices on dactyloscopy and DNA profiling is still unknown. In the current study, we demonstrate that procedures using Citrus Cleaner or sulfosalicylic acid were successful in removing ink and security smoke deposited on plastic plates but did result in the massive loss of fingerprint information as only a low number (4%) of good quality fingerprints were recovered after smoke contamination. Secondly, security ink Sun Blue ES2, but not SICPA Green and Sun Blue ES1, had a significant impact on DNA profiling success. DNA concentrations obtained from blood spiked swabs decreased with increasing ink concentration resulting in a complete loss of genotype information with the addition of ≥10 μl Sun Blue ES2. No noticeable PCR inhibition or DNA degradation was detected during quantification, but a decreased efficiency of DNA extraction could not be excluded. Security smoke, on the other hand, does not seem to have a significant influence on DNA analysis. Precautions must therefore be taken in order to avoid contaminating DNA swabs with ink during sampling. Thirdly, only a single negative impression of a glove in ink and a single glove print were able to be visualized with white fingerprint powder on detonated cash cassettes. In conclusion, the detection of glove prints and fingerprints is limited and security ink, contrary to smoke, after detonation can have a negative influence on downstream DNA analysis procedures.

Health Risks of Structural Firefighters from Exposure to Polycyclic Aromatic Hydrocarbons: A Systematic Review and Meta-Analysis.

Firefighters have an elevated risk of cancer, which is suspected to be caused by occupational and environmental exposure to fire smoke. Among many substances from fire smoke contaminants, one potential source of toxic exposure is polycyclic aromatic hydrocarbons (PAH). The goal of this paper is to identify the association between PAH exposure levels and contributing risk factors to derive best estimates of the effects of exposure on structural firefighters’ working environment in fire. We surveyed four databases (Embase, Medline, Scopus, and Web of Science) for this systematic literature review. Generic inverse variance method for random effects meta-analysis was applied for two exposure routes—dermal and inhalation. In dermal, the neck showed the highest dermal exposure increased after the fire activity. In inhalation, the meta-regression confirmed statistically significant increases in PAH concentrations for longer durations. We also summarized the scientific knowledge on occupational exposures to PAH in fire suppression activities. More research into uncontrolled emergency fires is needed with regard to newer chemical classes of fire smoke retardant and occupational exposure pathways. Evidence-based PAH exposure assessments are critical for determining exposure–dose relationships in large epidemiological studies of occupational risk factors.

Numerical Investigation of Smoke Extraction Methods in Large Storage Areas: Design Modifications

The design of smoke extraction is very import part in any fire system design. Where an incorrect design of the smoke extraction strategy may lead to delay in the response of the fire extinguishing systems, which causes the high rate of loss of life and destruction of the building and its contents. This work, numerically investigates the effectiveness of a smoke exhaust strategy in a large storage area using a Computational Fluid Dynamics (CFD) package named Fire Dynamics Simulator (FDS), which will be used in this work in order to simulate fires in the store, a fire will be designed at certain conditions to mimic the fire loads expected in the storage. Smoke movement from the origin of the fire, and smoke contamination in the store are simulated in order to obtain the optimal design conditions of smoke extraction strategy. The parameters that influence the smoke extraction in large storage, which will be studied in this work are: 1) temperature distributions inside the space with different scenarios of the fire onset, 2) air velocity according to different fire scenarios, and 3) distribution of fresh and exhaust air outlet. The results of these simulations are presented and discussed.

Review of the Effects of Grapevine Smoke Exposure and Technologies to Assess Smoke Contamination and Taint in Grapes and Wine

Grapevine smoke exposure and the subsequent development of smoke taint in wine has resulted in significant financial losses for grape growers and winemakers throughout the world. Smoke taint is characterized by objectional smoky aromas such as “ashy”, “burning rubber”, and “smoked meats”, resulting in wine that is unpalatable and hence unprofitable. Unfortunately, current climate change models predict a broadening of the window in which bushfires may occur and a rise in bushfire occurrences and severity in major wine growing regions such as Australia, Mediterranean Europe, North and South America, and South Africa. As such, grapevine smoke exposure and smoke taint in wine are increasing problems for growers and winemakers worldwide. Current recommendations for growers concerned that their grapevines have been exposed to smoke are to conduct pre-harvest mini-ferments for sensory assessment and send samples to a commercial laboratory to quantify levels of smoke-derived volatiles in the wine. Significant novel research is being conducted using spectroscopic techniques coupled with machine learning modeling to assess grapevine smoke contamination and taint in grapes and wine, offering growers and winemakers additional tools to monitor grapevine smoke exposure and taint rapidly and non-destructively in grapes and wine.

Polycyclic aromatic hydrocarbons in soybean grains

During the burning of the organic material (firewood) in the drying, combustion may occur incompletely, thus generating smoke contaminants such as polycyclic aromatic hydrocarbons (PAHs) in the furnaces. This study aimed to identify possible contamination by PAHs in soybean grains from storage units in municipalities in the Midwest region that have undergone drying in a direct-fired furnace with firewood as fuel. The soybean grains were collected in different municipalities of the Midwest region of Brazil, totaling 22 samples. A survey of possible contamination of soybean grains by PAHs was carried out using High-Performance Liquid Chromatography. The experiment was carried out in a completely randomized design with three replicates, and the data were subjected to analysis of variance, with means compared by Tukey test. The sum of the groups was: PAH4 - 1.45 μg kg−1 for Edéia (2), PAH8 - 2.97 μg kg−1 for Catalão (19) and PAHT - 5.06 μg kg−1 for Edéia (2); for benzo(a)pyrene, the sum was below 2.0 μg kg−1, not exceeded by the value of 0.64 μg kg−1 for Edéia (1). The values found of PAHs did not exceed the maximum limits established by the European Union, except for infant food.

Detection of smoke-derived compounds from bushfires in Cabernet-Sauvignon grapes, must, and wine using Near-Infrared spectroscopy and machine learning algorithms

The number and intensity of wildfires are increasing worldwide, thereby raising the risk of smoke contamination of grapevine berries and the development of smoke taint in wine. This study aimed to develop five artificial neural network (ANN) models from berry, must, and wine samples obtained from grapevines exposed to different levels of smoke: (i) Control (C), i.e., no misting or smoke exposure; (ii) Control with misting (CM), i.e., in-canopy misting, but no smoke exposure; (iii) low-density smoke treatment (LS); (iv) high-density smoke treatment (HS) and (v) a high-density smoke treatment with misting (HSM). Models 1, 2, and 3 were developed using the absorbance values of near-infrared (NIR) berry spectra taken one day after smoke exposure to predict levels of 10 volatile phenols (VP) and 18 glycoconjugates in grapes at either one day after smoke exposure (Model 1: R = 0.98; R2 = 0.97; b = 1) or at harvest (Model 2: R = 0.98; R2 = 0.97; b = 0.97), as well as six VP and 17 glycoconjugates in the final wine (Model 3: R = 0.98; R2 = 0.95; b = 0.99). Models 4 and 5 were developed to predict the levels of six VP and 17 glycoconjugates in wine. Model 4 used must NIR absorbance spectra as inputs (R = 0.99; R2 = 0.99; b = 1.00), while Model 5 used wine NIR absorbance spectra (R = 0.99; R2 = 0.97; b = 0.97). All five models displayed high accuracies and could be used by grape growers and winemakers to non-destructively assess at near real-time the levels of smoke-related compounds in grapes and/or wine in order to make timely decisions about grape harvest and smoke taint mitigation techniques in the winemaking process.

Assessment of Smoke Contamination in Grapevine Berries and Taint in Wines Due to Bushfires Using a Low-Cost E-Nose and an Artificial Intelligence Approach.

Bushfires are increasing in number and intensity due to climate change. A newly developed low-cost electronic nose (e-nose) was tested on wines made from grapevines exposed to smoke in field trials. E-nose readings were obtained from wines from five experimental treatments: (i) low-density smoke exposure (LS), (ii) high-density smoke exposure (HS), (iii) high-density smoke exposure with in-canopy misting (HSM), and two controls: (iv) control (C; no smoke treatment) and (v) control with in-canopy misting (CM; no smoke treatment). These e-nose readings were used as inputs for machine learning algorithms to obtain a classification model, with treatments as targets and seven neurons, with 97% accuracy in the classification of 300 samples into treatments as targets (Model 1). Models 2 to 4 used 10 neurons, with 20 glycoconjugates and 10 volatile phenols as targets, measured: in berries one hour after smoke (Model 2; R = 0.98; R2 = 0.95; b = 0.97); in berries at harvest (Model 3; R = 0.99; R2 = 0.97; b = 0.96); in wines (Model 4; R = 0.99; R2 = 0.98; b = 0.98). Model 5 was based on the intensity of 12 wine descriptors determined via a consumer sensory test (Model 5; R = 0.98; R2 = 0.96; b = 0.97). These models could be used by winemakers to assess near real-time smoke contamination levels and to implement amelioration strategies to minimize smoke taint in wines following bushfires.

Classification of Smoke Contaminated Cabernet Sauvignon Berries and Leaves Based on Chemical Fingerprinting and Machine Learning Algorithms

Wildfires are an increasing problem worldwide, with their number and intensity predicted to rise due to climate change. When fires occur close to vineyards, this can result in grapevine smoke contamination and, subsequently, the development of smoke taint in wine. Currently, there are no in-field detection systems that growers can use to assess whether their grapevines have been contaminated by smoke. This study evaluated the use of near-infrared (NIR) spectroscopy as a chemical fingerprinting tool, coupled with machine learning, to create a rapid, non-destructive in-field detection system for assessing grapevine smoke contamination. Two artificial neural network models were developed using grapevine leaf spectra (Model 1) and grape spectra (Model 2) as inputs, and smoke treatments as targets. Both models displayed high overall accuracies in classifying the spectral readings according to the smoking treatments (Model 1: 98.00%; Model 2: 97.40%). Ultraviolet to visible spectroscopy was also used to assess the physiological performance and senescence of leaves, and the degree of ripening and anthocyanin content of grapes. The results showed that chemical fingerprinting and machine learning might offer a rapid, in-field detection system for grapevine smoke contamination that will enable growers to make timely decisions following a bushfire event, e.g., avoiding harvest of heavily contaminated grapes for winemaking or assisting with a sample collection of grapes for chemical analysis of smoke taint markers.

Thirdhand Smoke Contamination and Infant Nicotine Exposure in a Neonatal Intensive Care Unit: An Observational Study.

Thirdhand smoke (THS) is ultrafine particulate matter and residue resulting from tobacco combustion, with implications for health-related harm (eg, impaired wound healing), particularly among hospitalized infants. Project aims were to characterize nicotine (THS proxy) transported on neonatal intensive care unit (NICU) visitors and deposited on bedside furniture, as well as infant exposure. Cross-sectional data were collected from participants in a metropolitan NICU. Participants completed a survey and carbon monoxide breath sample, and 41.9% (n = 88) of participants (n = 210) were randomly selected for finger-nicotine wipes during a study phase when all bedside visitors were screened for nicotine use and finger-nicotine levels. During an overlapping study phase, 80 mother-infant dyads consented to bedside furniture-nicotine wipes and an infant urine sample (for cotinine analyses). Most nonstaff visitors' fingers had nicotine above the limit of quantification (>LOQ; 61.9%). Almost all bedside furniture surfaces (93.8%) and infant cotinine measures (93.6%) had values >LOQ, regardless of household nicotine use. Participants who reported using (or lived with others who used) nicotine had greater furniture-nicotine contamination (Mdn = 0.6 [interquartile range, IQR = 0.2-1.6] µg/m2) and higher infant cotinine (Mdn = 0.09 [IQR = 0.04-0.25] ng/mL) compared to participants who reported no household-member nicotine use (Mdn = 0.5 [IQR = 0.2-0.7] µg/m2; Mdn = 0.04 [IQR = 0.03-0.07] ng/mL, respectively). Bayesian univariate regressions supported hypotheses that increased nicotine use/exposure correlated with greater nicotine contamination (on fingers/furniture) and infant THS exposure. Potential furniture-contamination pathways and infant-exposure routes (eg, dermal) during NICU hospitalization were identified, despite hospital prohibitions on tobacco/nicotine use. This work highlights the surreptitious spread of nicotine and potential THS-related health risks to vulnerable infants during critical stages of development. THS contamination is underexplored in medical settings. Infants who were cared for in the NICU are vulnerable to health risks from THS exposure. This study demonstrated that 62% of nonstaff NICU visitors transport nicotine on their fingers to the NICU. Over 90% of NICU (bedside) furniture was contaminated with nicotine, regardless of visitors' reported household-member nicotine use or nonuse. Over 90% of infants had detectable levels of urinary cotinine during NICU hospitalizations. Results justify further research to better protect infants from unintended THS exposure while hospitalized.

A Low-cost, Safe, and Effective Method for Smoke Evacuation in Laparoscopic Surgery for Suspected Coronavirus Patients.

Due to the recent COVID-19 pandemic, the debate regarding the safety of smoke evacuation during laparoscopic procedures and whether viruses can be aerosolized during such procedures has resurfaced.1 While the National Institute for Occupational Safety and Health Administration does not specifically require the use of smoke evacuation and filtering systems during laparoscopy,2 major concerns for surgical staff safety have been raised concerning all aerosol generating procedures (AGP). Since hepatitis B virus (HBV) has previously been demonstrated to be present in surgical smoke in 10 out of 11 HBV positive patients,3 it is feared that the Covid-19 virus could also be disseminated during AGPs and potentially infect the surgical staff. Although the COVID-19 virus (SARS-CoV-2) has not yet been detected in AGPs, care should be taken to reduce the risk of surgical staff infection in the operating room. In experimental conditions SARS-Cov-2 has recently been shown to have aerosol and fomite transmission potential similar to SARS-Cov-1 (the most closely related human virus), in particular remaining in aerosols for 3 hours or more and on surfaces for up to 72 hours.4 In anticipation of a substantial amount of operations in the near future of COVID-19 carriers and infected patients, a simple and effective measure for the evacuation and entrapment of smoke and aerosol is needed. Due to collapsing economies and limited availability for appropriate filter equipment within this pandemic, we suggest the use of a simple, very low-cost filtration system with readily available components in the operating room for immediate implementation. Standard electrostatic filters used for ventilation machines have the capability of filtering known bacterial and viral loads with great efficiency5 and most are certified for 99.99% effective protection against HBV and hepatitis C virus which have a diameter of 42 nm and 30 to 60 nm respectively.6 SARS-CoV-2 has a larger diameter of 70 to 90 nm7 therefore the same filtering efficiency can be expected to apply for new virus. This filter can be connected via standard tubing to the trocar evacuation port to constitute an evacuation and filtering system which evacuates the generated smoke, as well as filter the potential viral load to ensure surgical staff safety. To connect the filter to the tubing we use the endotracheal tube connector (Fig. 1A, B). No active suctioning is attached to this system.FIGURE 1: (A) All the standard OR equipment needed to assemble the system including ventilation machine filter, endotracheal tube connector, drainage tube connector, and IV tubing; and (B) the complete filtering system assembled.Following simple bench top studies evaluating the capability of evacuating smoke effectively, the system was recently used clinically in 5 operations including cholecystectomy, inguinal hernia repair, Total Mesorectal Excision, Transanal Total Mesorectal Excision, and anterior resection of the rectum by surgeons in Israel and Italy. Consulted online via the current social media possibilities, all surgeons within the European Association for Endoscopic Surgery technology committee reported simple and quick assembly of the system as well as very good efficiency of smoke evacuation during their laparoscopic procedures. As this is a passive system, it is recommended to use short and wide tubing to decrease flow resistance. The filter system should be discarded according to hospitals’ protocols for infection control. Dissemination of knowledge and exchange of ideas about this rapidly spreading infection is crucial to healthcare workers globally. Findings and guidelines are quickly being published and frequently updated by relevant learned societies such as the American College of Surgery, the Society of American Gastrointestinal and Endoscopic Surgeons, and the Royal College of Surgeons 8–10 to update surgeons dealing with COVID-19. Although the Royal College of Surgeons of Edinburgh published important guidance of surgical care to protect patients and surgeons,10 we disagree with the statement that laparoscopy should be avoided. Provided a simple, safe, and reliable filtering and evacuation system for pneumoperitoneum gases is used, and that strict precautions are abided to, laparoscopy is potentially preferable to open surgery, where smoke contamination is uncontrollable. Suggestions such as instrument cleaning during surgery, deflating pneumoperitoneum prior to removing trocars, conversion or specimen extraction, as highlighted by the Chinese and Italian experience, are particularly helpful.1 Social media is essential and largely used for exchange of experiences and fast implementation worldwide. Websites of leading societies like Society of American Gastrointestinal and Endoscopic Surgeons, European Association for Endoscopic Surgery, American College of Surgery are updated daily, while surgeons share their own experiences and ideas via Twitter, LinkedIn, and Facebook which instantly disseminate knowledge. Videos demonstrating assembling of the filtering system (video 1, https://links.lww.com/SLA/C205) and its effectiveness during laparoscopic operations (video 2, https://links.lww.com/SLA/C206) are attached and can be used for widespread dissemination. Eagerly awaited are clinical data to determine if this proposed solution is effective in preventing contamination in the operating room or not.