The paper deals with the bicycle center infrastructure in a city, which becomes popular every year, but is not yet studied properly. Little attention is devoted to the spatial development for two-wheeled vehicles, so there are many problems to be discussed and solved.The purpose of this paper is to determine the types of bicycle centers in the structure of a city, from the main and central to regional and peripheral that connect all urban areas. Moving along the bicycle paths from home to a large bicycle center and back to home, one can communicate in bicycle centers. Less functional peripheral centers provide repair services, bicycle rental and small recreation. The city of Rostov-on-Don is taken as an example.The following parameters are determined: optimum distance between bicycle centers of different types, their functional content, bicycle center location in the city. In the future, it is possible to arrange such centers in any city due to their unification.The model of the bicycle center network is proposed for the city of Rostov-on-Don, that are characterized by different area and multifunctional purposes for each district to attract people. The main bicycle center includes sports grounds, bicycle track, rock climbers, skate park, gyms, ski jumping hall and other sports facilities. Roof-top bike trails, amphitheater with a street stage, art spaces, go-karting can be arranged on the territory of these centers.