Small Bead Size Research Articles

Occurrence, Related Factors and Prognostic Value of Vascular Lake in Hepatocellular Carcinoma Patients Treated with Drug-Eluting Bead Transarterial Chemoembolization.

PurposeThe present study aimed to assess the prevalence of vascular lake (VL), its associated factors and correlation with prognosis in hepatocellular carcinoma (HCC) patients treated with drug-eluting bead transarterial chemoembolization (DEB-TACE). Patients and Methods A total of 286 primary HCC patients (with 384 treated nodules) receiving DEB-TACE treatment were recruited, and their clinical characteristics were documented. The occurrence of VL was recorded, and treatment responses were assessed according to the modified response evaluation criteria in solid tumor (mRECIST).In terms of treatment response, the total response status (including CR, PR, SD and PD), objective response rate (ORR) and disease control rate (DCR) were elevated in VL patients compared to non-VL patients as well as in VL nodules compared to non-VL nodules. Liver function indexes and adverse events were assessed. Progression-free survival (PFS) and overall survival (OS) were evaluated with the last follow-up date of March 2020. ResultsThe patient-based and nodule-based VL occurrence rates were 17.1% and 16.4%, respectively. Larger tumor size, pseudocapsules and smaller bead size were independently associated with VL occurrence. PFS and OS were more prolonged in VL patients than in non-VL patients, and VL independently correlated with better PFS and OS. For liver function, the liver function indexes before and after DEB-TACE were of no difference between VL patients and non-VL patients. Additionally, the incidences of adverse events were similar between VL patients and non-VL patients.Conclusion VL occurs in 17.1% of HCC patients treated with DEB-TACE, and it is correlated with larger tumor size, pseudocapsule, smaller bead size, more favorable treatment response and better survival.

The development of laminin-alginate microspheres encapsulated with Ginsenoside Rg1 and ADSCs for breast reconstruction after lumpectomy

Many technologies have been developed for breast reconstruction after lumpectomy. Although the technologies achieved promising success in clinical, there are still many shortages hanging over and trouble the researchers. Tissue engineering technology was introduced to plastic surgery that gave a light to lumpectomy patients in breast reconstruction. The unexpected absorption rate, resulting from limited vascularization and low cell survival rate, is a major factor that leads to unsatisfactory results for the previous studies in our lab. In the study, the laminin-modified alginate synthesized by a new method of low concertation of sodium periodate would be mixed with ADSCs and Rg1 in the medium; and then sprayed into a calcium chloride (CaCl2) solution to prepare into microsphere (abbreviated as ADSC–G-LAMS) by bio-electrospray with a power syringe for the mass production and smaller bead size. The developed ADSC–G-LAMS microspheres had the diameter of 232 ± 42 μm. Sustained-release of the Rg1 retained its biological activity. WST-1, live/dead staining, and chromosome aberration assay were evaluated to confirm the safety of the microspheres. In in vivo study, ADSC–G-LAMS microspheres combined with autologous adipocytes were transplanted into the dorsum of rats by subcutaneous injection. The efficacy was investigated by H&E and immunofluorescence staining. The results showed that the bioactive ADSC–G-LAMS microspheres could integrate well into the host adipose tissue with an adequate rate of angiogenesis by constantly releasing Rg1 to enhance the ADSC or adipocyte survival rate to join tissue growth and repair with adipogenesis for breast reconstruction after lumpectomy.

Effect of Processing Parameters on the Diameter and Morphology of Electrospun Iron-Modified Montmorillonite (Fe-MMT)/Polycaprolactone Nanofibers

Iron-modified montmorillonite-filled polycaprolactone nanofiber mats were produced via electrospinning with varying applied voltage, flow rate, needle-tip-to-collector distance, and needle diameter. Scanning electron microscopy (SEM) was used to observe fiber morphology and characteristics. The effects of varying process parameters on various fiber characteristics were evaluated using a two-level fractional factorial experimental design. The effect of voltage on fiber diameter differed with varying flow rate. At 32 ml/hr, the average fiber diameter decreased from 518.38 nm ± 289.37 nm to 466.43 nm ± 312.36 nm when the voltage is increased. At 42 ml/hr the effect of voltage on fiber diameter was reversed. The average fiber diameter was also found to decrease from 516.03 nm ± 283.48 nm to 467.96 nm ± 318.07 nm with decreasing tip-to-collector distance at 32 mL/hr flow rate. The variation of the effect of the factors on fiber diameter was mainly due to a significant loss of material observed at 12 kV and 15 cm tip-to-collector distance. Bead formation was observed for all runs with more beads being formed at 12 kV applied voltage and 15 cm tip-to-collector distance. Spherical beads were observed at 12 kV and 15 cm tip-to-collector distance while spindle-like beads were present in nanofiber membranes spun at high voltage and at the combination of low voltage and low tip-to-collector distance. The parameter setting combination of 19 kV, 32 ml/hr flow rate, 10 cm tip-to-collector distance, and 0.514 mm needle diameter yielded the lowest fiber diameter with the least amount of beading and small bead size. Small fiber diameters and less beading provide larger surface area and more exposure of the Fe-MMT particles for more efficient adsorption.

Abstract No. 670 Optimal delivery rate and concentration of radiopaque beads for trans-arterial embolization

To determine how changes in the standard injection rate (1.0 mL/min), in the standard concentration (1:9), or the size of the LUMI beads affect the embolization volume. A total of 9 Yorkshire pigs underwent fluoroscopy guided injection of LUMI radiopaque beads using 2 different LUMI bead sizes (R0=40-90 and R1=70-150 microns). Each animal was injected with 1 of 3 bead / Visipaque 320 contrast concentrations (1:4, 1:9, and 1:14) and at 1 of 3 injection rates (0.5mL/min, 1.0mL/min, and 1.5mL/min). Isovue contrast was used to position the catheter and allow for direct injection of the embolization beads into the arterial branch of the liver and kidneys. The larger LUMI beads (70-150 microns) were used to embolize the left kidneys and left hepatic lobes and the smaller LUMI beads (40-90 microns) were used to embolize the right kidneys and right hepatic lobes. The animals were subsequently euthanized. The distance of the beads from the hilum was then evaluated with imaging and pathological correlation. Technical success rate was 100% (36/36). Embolization was achieved regardless of the injection rate and concentration. The complication rate after the embolization process was 0% (0/36). When measuring the effect of concentration on embolization volume, higher concentrations correlated with decreased embolization volumes in both the liver and kidney (P = .018 and P = .001, respectively). Smaller bead sizes also correlated with decreased embolization volume in both the liver and kidney (P = .014 and P = .048, respectively). However, different injection rates did not result in a statistically significant difference in embolization volume in either the liver or kidney (P = .393 and P = .338, respectively). Increased concentration and smaller bead sizes appear to decrease embolization volume. However, changes in the injection rate do not appear to affect the embolization volume.

3:27 PM Abstract No. 305 Vascular selectivity of radiopaque beads during transarterial embolization

To determine if LUMI bead size, injection rate, or concentration affects the ability to embolize blood vessels further into the periphery of the organ. A total of 9 Yorkshire pigs underwent fluoroscopy-guided injection of LUMI radiopaque beads using 2 different LUMI bead sizes (R0 = 40-90 and R1 = 70-150 microns). Each animal was injected with 1 of 3 bead/Visipaque320 contrast concentrations (1:4, 1:9, and 1:14) and at 1 of 3 injection rates (0.5 mL/min, 1.0 mL/min, and 1.5 mL/min). Isovue contrast was used to position the catheter and allow for direct injection of the embolization beads into the arterial branch of the liver and kidneys. The R1 and R0 beads were used to embolize the left kidney and left hepatic lobe and the right kidney and right hepatic lobe, respectively. The animals were subsequently euthanized. The distance of the beads from the hilum and the capsule was then evaluated with imaging and pathological correlation. Technical success rate was 100% (36/36). Embolization was achieved regardless of the injection rate and concentration. The complication rate after the embolization process was 0% (0/36). In the liver, the beads traveled more distally from the hilum for lower concentrations (P = .008), but this was not statistically significant for the kidney (P = .279). When comparing the distance traveled by the beads from the hilum with changes in the injection rate, the difference was not statistically significant for the liver or kidney (P = .985 and P = .907, respectively). When comparing the distance traveled by the beads from the hilum with changes in the bead size, the difference was not statistically significant for the liver or kidney. (P = .779 and P = .244, respectively). However, in the kidney, when measuring the distance from the beads to the capsule, lower bead sizes demonstrated greater peripheral distribution (P = .007). Lower concentrations and smaller bead sizes appear to demonstrate a more peripheral distribution, although this may be organ dependent. However, bead distribution does not appear to be affected by injection rate.

Impact of a severe rain event on C- and N-DBP precursor removal using IEX

Abstract During disinfection, dissolved organic matter (DOM) is the major precursor to form disinfection by-products (DBPs), which may be of potential human health concern. Previous research focused on waters of continental climates and less on subtropical environments. However, water sources in subtropical climates are regularly impacted by major rain events during the summer months. This study evaluated the C- and N-DBP precursor removal capacity of two conventional ion exchange (IEX) resins and one magnetic ion exchange (MIEX) resin with a raw water at normal conditions and impacted by a heavy rain event. The rain event introduced 3 mg C/L total organic carbon (TOC) comprised mainly of low to medium molecular weight organics. All three resins were able to remove TOC and DBP precursors (>66%) but being less efficient in reducing turbidity (3–48%) and colour (9–24%). The resin with the smallest bead size was affected the most by the increased medium MW DOM loading resulting in DOM and C-DBP precursor removal performance losses of 10% and 22%. When applied as a pre-treatment for coagulation, MIEX was more efficient in DBP precursor control than coagulation in addressing the additional organic and DBP precursor loading after a heavy rain event.

Downstream drug product processing of itraconazole nanosuspension: Factors influencing tablet material properties and dissolution of compacted nanosuspension-layered sugar beads

There has been limited research done on the downstream processing of nanosuspensions into solid oral dosage forms. This paper demonstrates the bead layering process with a layering level at 150% and 240%, as well as the selection and justification of the outer phase excipients for tabletability and disintegrating properties. In a previous study, an itraconazole nanosuspension stabilised by SDS and HPMC E5 was layered onto sugar beads with coating polymer HPMC VLV. In the current study, compression studies with these layered beads utilising the small bead size at 150% or 240% layering levels with outer phase cushioning excipients MCC, copovidone or isomalt were performed. Other excipients such as co-compressed crospovidone-PEG 4000; DCP functioning as a disintegrant; and HPC as a binder was also added. Target output variables were achieved with a balance between an adequate tensile strength and fast dissolution rate with a release of 99.0% (±1.0% SD) within 10min, which is in accordance with the FDA guidance for dissolution testing. The results show that the compaction of nanosuspension-layered beads is a suitable process for processing an itraconazole nanosuspension into a solid dosage form such as a compacted tablet without compromising on drug release.

Downstream drug product processing of itraconazole nanosuspension: Factors influencing drug particle size and dissolution from nanosuspension-layered beads

There is more research required to broaden the knowledge on the downstream processing of nanosuspensions into solid oral dosage forms, especially for coated nanosuspensions onto beads as carriers. This study focuses on bead layering as one approach to solidify nanosuspensions. The aim was to systematically investigate the influence of type of coating polymer (HPMC VLV vs. copovidone), bead material and bead size (sugar vs. MCC, and small vs. large) and coating thickness (50%–150% layering level) on the properties of a dried itraconazole nanosuspension. A stable itraconazole nanosuspension with a mean particle size below 200nm was prepared and a ratio of itraconazole and coating polymer of around 1:1 was identified. XRD and DSC scans revealed that itraconazole remained mostly crystalline after the bead layering process. The fastest dissolution rate was achieved using the small bead size, sugar beads, HPMC VLV as film-forming polymer and lowest layering level, with the best formulation releasing 94.1% (±3.45% SD) within the first 5min. A deterioration of the release profile with increasing layering level was only observed for MCC beads and was more pronounced when copovidone was used as a coating polymer. It was observed that bead layering is a suitable method to process an itraconazole nanosuspension into a solid form without compromising release.

The smaller, the better? The size effect of alginate beads carrying plant growth-promoting bacteria for seed coating

A range of lab-scale methods for encapsulation of plant growth-promoting bacteria in alginate beads intended for seed coating was evaluated: contact-spotting, extrusion through syringe with/without vibration, ejection by robotic liquid handler, extrusion by centrifugal force and commercial devices (nanodispenser, aerodynamically assisted jetting, encapsulator). Two methods were selected based on throughput (encapsulator: 1.5–5 mL/min; syringe with subsequent pulverisation: 5 mL/min). Four bead sizes (55 ± 39 μm, 104 ± 23 μm, 188 ± 16 μm and 336 ± 20 μm after lyophilisation) were produced. Bacterial viability, release, bead morphology, seed surface coverage and attrition were investigated. Release from the smallest bead size was approximately 10 times higher than from the largest. Seed surface coverage was highest (69 ± 3%) when alginate beads produced with nozzle size 80 μm were applied. Pulverised macro-beads are an alternative option, if high throughput is top priority.

Potential mechanisms for bioregeneration of perchlorate-containing ion-exchange resin

Ion-exchange (IX) is the most feasible technology for perchlorate removal from drinking water. Reuse of resins present challenges, however. Selective resins are non-regenerable, and are incinerated after one time use, while non-selective resins, when regenerable, produce a waste stream that contains high concentration of perchlorate that must be disposed of. A process to bioregenerate spent resin containing perchlorate with perchlorate-reducing bacteria (PRB) has been recently developed. In this research, potential mechanisms for bioregeneration of resin-attached perchlorate (RAP) were investigated. Batch bioregeneration experiments were performed using gel-type and macroporous-type resins. Various initial chloride concentrations and various resin bead sizes were used. The results of the bioregeneration experiments suggested that chloride, i.e. the product of perchlorate biodegradation, is more likely the desorbing agent of RAP; and increasing the concentration of chloride enhances the bioregeneration process. Both film and pore diffusion were found to be relevant with respect to the rate of perchlorate mass-transfer to the bulk liquid. Bioregeneration was found to be more effective for macroporous than for gel-type resins, especially in the case of macroporous resins with relatively small bead size in the presence of higher chloride concentration.

Divinyl Sulfone으로 가교된 히알루론산 마이크로비드의 특성평가

Hyaluronic acid(HA) microbeads were synthesized by dropping the sodium hyaluronate(Streptococcus) solutions in NaOH into a solution mixture of divinyl sulfone(DVS) in 2-methyl-1-propanol, followed by stirring, cleaning and drying process at room temperature. The initial experimental conditions are crosslinking time(CLTi) of 5 h, crosslinking temperature(CLTe) of room temperature, injection air pressure(IAPr) of 5 psi, and DVS concentration( DVSc) of 0.2 vol%, respectively. Then, parametric studies were performed by varying the parameters to investigate the morphology, the porosity, the swelling ratio and the size of the beads. The microbead size pattern was not regular to function of the degree of crosslink. It was observed that the swelling ratio, the degree of crosslink, and the pore size can be controlled by adjusting the CLTi, CLTe and DVSc. Among the parameters investigated, the smallest bead size can be achieved by varying the CLTi parameter. The lowest swelling ratio, as an indication of the highest degree of crosslink, can be obtained by varying CLTe.

Specific peptides as alternative to antibody ligands for biomagnetic separation of Clostridium tyrobutyricum spores

Nowadays, the reference method for the detection of Clostridium tyrobutyricum in milk is the most-probable-number method, a very time-consuming and non-specific method. In this work, the suitability of the use of superparamagnetic beads coated with specific antibodies and peptides for bioseparation and concentration of spores of C. tyrobutyricum has been assessed. Peptide or antibody functionalized nanoparticles were able to specifically bind C. tyrobutyricum spores and concentrate them up to detectable levels. Moreover, several factors, such as particle size (200 nm and 1 μm), particle derivatization (aminated and carboxylated beads), coating method, and type of ligand have been studied in order to establish the most appropriate conditions for spore separation. Results show that concentration of spore is favored by a smaller bead size due to the wider surface of interaction in relation to particle volume. Antibody orientation, related to the binding method, is also critical in spore recovery. However, specific peptides seem to be a better ligand than antibodies, not only due to the higher recovery ratio of spores obtained but also due to the prolonged stability over time, allowing an optimal recovery of spores up to 3 weeks after bead coating. These results demonstrate that specific peptides bound to magnetic nanoparticles can be used instead of traditional antibodies to specifically bind C. tyrobutyricum spores being a potential basis for a rapid method to detect this bacterial target.

Investigation of Immobilization of Functionalized Magnetic Nanobeads in Rolling Circle Amplified DNA Coils

Immobilization characteristics for single-stranded oligonucleotide-functionalized magnetic beads with nominal sizes of 40, 80, 130, and 250 nm in rolling circle amplified (RCA) DNA coils is investigated by employing complex magnetization measurements, dynamic light scattering and fluorescence microscopy. It was found that larger beads in a polydisperse bead size distribution more easily immobilize in the RCA DNA coils than do smaller beads. This may be related to a higher oligonucleotide surface coverage for the larger beads. Furthermore, it was concluded that both bead size and oligonucleotide surface coverage determine whether beads immobilize to give isolated coils with beads or larger clusters of beads and coils. A small bead size and a low oligonucleotide surface coverage favor the first kind of immobilization behavior, whereas a large bead size and a high oligonucleotide surface coverage favor the other. The present findings could be used to optimize both size and surface functionalization of beads employed in substrate-free magnetic biosensors.

Isocratic rapid liquid chromatographic method for simultaneous determination of carotenoids, retinol, and tocopherols in human serum

An improved isocratic and rapid HPLC method was developed for the measurement of carotenoids, retinol and tocopherols in human serum. Vitamins were extracted with hexane. Mobile phase consisted of a mixture acetonitrile:methylene chloride:methanol with 20 mM ammonium acetate. This method used a small bead size (3 μm) Spherisorb ODS2 column with titane frits. Diode array and fluorescence detectors were used respectively for the detection of carotenoids and retinol/tocopherols. Chromatographic separation was complete in 13 min for β-cryptoxanthin, cis–trans-lycopene, α-carotene, β-carotene, cis-β-carotene, retinol, δ-tocopherol, γ-tocopherol and α-tocopherol. Echinenone and tocol were employed as internal standards for diode array and fluorescence detection. Accuracy was validated using standard reference material (SRM) 968C. Intra-assay and inter-assay precision were respectively 0.2–7.3% and 3.6–12.6%. Sensitivity was verified using the ICH recommendations and the limit of detection (LOD) obtained was sufficient for routine clinical application.

Effect of High Surface Curvature on the Main Phase Transition of Supported Phospholipid Bilayers on SiO2 Nanoparticles

Investigation of the physical properties of highly curved membranes is important in biology, for example, in fusion intermediates, and in pharmaceutical or chromatographic applications, where nanoscale features may affect substrate binding. However, vesicle fusion below 40 nm precludes study of this size regime. In this investigation, the effect of high surface curvature on the adsorption and morphology of phosphotidylcholine lipids with alkyl chain lengths of 14 (DMPC), 16 (DPPC), and 18 (DSPC) onto silica (SiO2) nanobeads was investigated by thermogravimetric analysis (TGA), high sensitivity nanocalorimetry, and vibrational spectroscopy. The SiO2 beads ranged in size from 5 to 100 nm. Stable supported bilayers were formed on all bead sizes by vesicle fusion of the parent MLVs at temperatures above the main phase transition temperature (T(m)) of the lipids. A downward shift in T(m), and a broadening (deltaT1/2) of the transition with respect to the parent MLVs, was observed for the 100 nm beads. With decreasing bead size, T(m) first decreased, but then increased. On the smallest bead size, whose dimensions were comparable to those of the adsorbed lipids, T(m)'s were higher than those of the parent MLVs. The increase in T(m) indicated a stiffening of the supported bilayer, which was confirmed by Raman spectroscopic data. Narrowing of the phase transition or the appearance of peak doublets occurred at the smaller bead sizes. The results were consistent with a model in which the high free volume and increased outer headgroup spacing of lipids on highly curved surfaces induced interdigitation in the supported lipids.

Milk Cholesterol Reduction Using Immobilized Lactobacillus acidophilus ATCC1643 in Sodium-Alginate

Lactobacillus acidophilus is one of the major microorganisms which are famous for their effects on cholesterol. In this study, we have investigated the effect of L. acidophilus ATCC 1643 on removing the milk cholesterol and additionally, we have immobilized L. acidophilus ATCC1643 cells in sodium-alginate and observed its effect on milk cholesterol removing. Also, we have researched about the effect of some factors including: bacteria cells number (both free and immobilized cells) and immobilized cells bead size on cholesterol removing rate and ultimately the extracted results were compared together. The results indicated that free cells could reduce cholesterol to lower than 0.5mg/100ml milk. Also, smaller bead size resulted higher cholesterol reduction. Increasing the number of immobilized cells showed significant effect on cholesterol removal to about 86%. Also, reusability of the beads showed that immobilized cells are active in cholesterol processing for three times.

Purification of MBP‐tagged proteins using new prepacked columns

The success of protein production and subsequent analysis are dependent on the expression levels, solubility and purification of the protein. Maltose Binding Protein (MBP) is often used as an affinity fusion tag. Several studies have shown that MBP‐tagged proteins have high solubility compared to other tagged proteins.The new MBPTrap™ HP consists of an affinity medium, Dextrin Sepharose™ High Performance, packed in 1‐ml and 5‐ml HiTrap™ columns. The medium has high specificity and capacity. Additionally, it can withstand harsh Cleaning In Place (CIP) procedures, like sodium hydroxide, without losing binding capacity. The HiTrap format enables automated protocols to be used on ÄKTAdesign™ systems as well as with syringe or laboratory pump.In this work, MBP‐tagged proteins from E. coli were purified on prepacked 1‐ml or 5‐ml MBPTrap HP columns. The purity of the eluted target proteins was greater than 90 %. A stability study was performed, six purification runs each followed by a CIP run (0.5 M NaOH) were made on the same column. The results show no significant change in yield or purity. The results of an automated two step purification on ÄKTAxpress™ is shown. The affinity chromatography step on MBPTrap HP was followed by a gel filtration step.In summary, the results show that the prepacked MBPTrap HP column has high binding capacity and the eluted target protein is very pure. Furthermore, the medium is stable in 0.5 M NaOH.

Aesthetic Diode Laser Welding of Stainless Steel

In the present study the use of diode laser for stainless steel aesthetic welding is considered. In fact the surface smoothness of the weld bead, attributed to the mode stability of the laser system, suggests its application for aesthetic weld joint. The aims of this work were to: define a process map, based on an aesthetic criterion; mechanically characterize the weld joints (by Vickers micro-hardness and tensile test); analyse the surface of the specimens in terms of roughness. Moreover, gas tungsten-arc (TIG) weld joints were carried out and analogously tested to make a comparison with the diode laser results. Good aesthetic butt joints were obtained with diode laser welding. The same results were found working with constant power speed ratio (i.e. constant fluence). Comparison between TIG and Laser Beam Welding (LBW), showed no significant difference in roughness, while ultimate tensile load was higher for TIG welding. The differences in joint strength depend on the different cross-sectional area and on the extension and microstructure of the base metal, heat-affected zone and melted zone. However, diode laser technology allows to obtain smaller bead size.

Mechanical Properties of Glass Bead-Filled Linear Low-Density Polyethylene

The tensile and impact properties of glass bead (GB)-filled linear low-density polyethylene (LLDPE) were studied in this article. The tensile stress–strain behaviors of GB-filled LLDPE composites do not show any obvious difference from that of pure LLDPE, but the overall mechanical properties of GB-filled LLDPE composites are improved quite a lot, which demonstrates that in a relatively small bead size range, the inorganic spherical particle can effectively increase the mechanical properties of LLDPE. The elastic modulus and the elongation to break are almost constant for various filler contents, while the yield strength greatly increases when the filler content is very low, and then remains at about that level up to a 20% filler concentration. After a sharp increase when the filler concentration is 5% by weight, the impact strength gradually increases with increasing filler content, and obtains a 40% improvement above the resin, showing significant toughening effect. Combining the effects of bead size and filler concentration studied on the mechanical properties of GB-filled LLDPE composites, it is found that for glass beads with relatively small particle size, the bead size plays a significant role, which seems to be inconsistent with some results from the literature. From investigation of the morphology of the filled specimen, it is found that though the glass beads are not surface treated, the glass beads used present quite good interfacial adhesion with the matrix LLDPE, and the distribution of the bead in the matrix is the main influencing factor on the mechanical properties of the LLDPE composites.

Experimental and numerical study of microwave drying in unsaturated porous material

The drying of unsaturated porous material due to microwave drying (2.45GHz) has been investigated numerically and experimentally. Most importantly, this work focuses on the influence of moisture content on each mechanism (vapor diffusion and capillary flow) during microwave drying process. Based on a model combining the electric field and heat-mass transport equations show that the variation of initial moisture content and particle size changes the degree of penetration and rate of absorbed energy within the material. The small bead size leads to much higher capillary forces resulting in a faster drying time.