Small Accumulation Research Articles

Geochemical interpretation of partially filled hydrocarbon traps in the nearshore Gippsland Basin

This study aims to analyse the relative importance of competing processes in determining the distribution of liquid and gaseous hydrocarbon accumulations in the nearshore Gippsland Basin. These processes include primary source/charge factors such as kerogen type and maturity at the time of expulsion, fractionation processes during migration, and secondary alteration effects such as biodegradation and/or water-washing in the presence of a strong low-salinity water-wedge that has pushed out 20–40 km from the coastline during Quaternary time. The authors find that small accumulations of liquid petroleum predominate west of Emperor, Snapper, and Bream. These small accumulations show evidence of primary charges of wet-gas, and various stages of in-reservoir alteration. Compared to liquids in the Central Deep and in the basal oil-legs of the giant wet-gas fields at Snapper and Marlin, wax contents are low, and API gravities are high, close to the Emperor-Bream line, but then decrease westwards towards the coastal region. Oil bubble-point pressures decrease westwards (despite shallower burial than the Bream, Snapper, and Marlin fields, further offshore), due to increasing under-saturation with respect to dissolved gas. The authors note that the dominance of gas in the Barracouta Field is probably due to strong recharge with unaltered wet gas but that dry gas in the Golden Beach Field suggests biogenic methane, as proven for the Baleen gasfield by carbon isotopes. The authors infer that the presence or absence of thin oil-legs in the Barracouta, Snapper, Marlin and Bream wet gas fields reflects a balance of water-washing and fresh charge. Residual oil shows below the Dolphin and Perch OWCs, and a lost column at Amberjack–1, demonstrate volume reduction following peak charge. The authors, therefore, conclude that observed underfill of structures is generally a result of in-reservoir processes post-charge, rather than a deficiency of charge or seal.

Subglacial hydrological connectivity within the Byrd Glacier catchment, East Antarctica

AbstractIce, Cloud and land Elevation Satellite (ICESat) repeat-track laser altimetry has identified 17 sites within the Byrd Glacier catchment, East Antarctica, where rapid ice-surface height changes have occurred, which have been interpreted as evidence for ‘active’ subglacial lakes. Here we present evidence from a new radio-echo sounding (RES) survey at 11 of these locations to understand the bed conditions associated with the proposed hydrological activity. At none of the sites examined did we find evidence in support of substantial pooled basal water. In the majority of cases, along-track RES bed reflection amplitudes either side of the locations of surface height change are indistinguishable from those within the features. These results indicate that, in most cases, hypothesized ‘active’ lakes are not discrete radar targets and are therefore much smaller than the areas of surface height change. In addition, we have identified three new relatively large subglacial lakes upstream of the region where most ‘active’ subglacial lakes are found, in an area where the hydraulic gradient is significantly lower. Our results suggest that substantial and long-lasting basal water storage in the Byrd Glacier catchment occurs only under low hydraulic gradients, while coast-proximal sites of hydraulic activity likely involve small or temporary accumulations of basal water.

Application of diffracted wave analysis to time‐lapse seismic data for CO2 leakage detection

ABSTRACTTime‐lapse seismic analysis is utilized in CO2 geosequestration to verify the CO2 containment within a reservoir. A major risk associated with geosequestration is a possible leakage of CO2 from the storage formation into overlaying formations. To mitigate this risk, the deployment of carbon capture and storage projects requires fast and reliable detection of relatively small volumes of CO2 outside the storage formation. To do this, it is necessary to predict typical seepage scenarios and improve subsurface seepage detection methods. In this work we present a technique for CO2 monitoring based on the detection of diffracted waves in time‐lapse seismic data. In the case of CO2 seepage, the migrating plume might form small secondary accumulations that would produce diffracted, rather than reflected waves. From time‐lapse data analysis, we are able to separate the diffracted waves from the predominant reflections in order to image the small CO2 plumes. To explore possibilities to detect relatively small amounts of CO2, we performed synthetic time‐lapse seismic modelling based on the Cooperative Research Centre for Greenhouse Gas Technologies (CO2CRC) Otway project data. The detection method is based on defining the CO2 location by measuring the coherency of the signal along diffraction offset‐traveltime curves. The technique is applied to a time‐lapse stacked section using a stacking velocity to construct offset‐traveltime curves. Given the amount of noise found in the surface seismic data, the predicted minimum detectable amount of CO2 is 1000–2000 tonnes. This method was also applied to real data obtained from a time‐lapse seismic physical model. The use of diffractions rather than reflections for monitoring small amounts of CO2 can enhance the capability of subsurface monitoring in CO2 geosequestration projects.

Travertine: Distinctive depositional fabrics of carbonates from thermal spring systems

AbstractThe terrestrial limestones forming at the emergence of thermal springs show a variety of unusual depositional facies. The specific lithological and petrological features of these deposits have few counterparts in the marine, and continental, karst‐related carbonates, but they are typical of the epigean limestone that has been quarried since antiquity in the surroundings of Tivoli (Rome) under the name of travertine, where it is still forming in hydro‐geothermal fields linked to extensional tectonics. The physicochemical, hydrodynamic and geological conditions specific to the Tivoli thermal spring system imply hypersaturated alkaline–sulphate, warm to hot waters, upwelling from springs fed through open fractures/faults in extensional and/or volcanic regimes. These features, together with the hydrodynamic behaviour of the water flows running from the vents, control the petrogenetic features of the travertine, a well‐bedded, mostly finely laminated, porous but quite compact limestone. The results of a detailed comparative petrological analysis carried out on the lithofacies of travertine limestones, and of those observed during formation within numerous active thermal spring fields, provide the elements required for an exhaustive textural classification of the travertine lithofacies, which has not yet been described systematically. According to the genetic processes and fabrics, the thermal deposits that originate from such hypersaturated alkaline–sulphate, warm to hot waters, can be subdivided into: abiotic crystalline crusts, microbially mediated crusts (microbialites) and granular deposits mostly represented by small accumulations of lime‐mudstone. Some of the granular deposits and the microbialites are only partially comparable with analogous sediments forming on tidal flats/sabkhas or other continental sites; however, the facies association of crystalline crusts and laminar curled microbialites has no counterpart in the marine realm. The widespread presence of thermophile bacteria and sulphobacteria, and the general absence of autochthonous eukaryote organisms, unable to live in poisonous sulphate waters, are also undeniable evidence of their thermal origin.

Non-CG methylation patterns shape the epigenetic landscape in Arabidopsis.

DNA methylation occurs in CG and non-CG sequence contexts. Non-CG methylation is abundant in plants, and is mediated by CHROMOMETHYLASE (CMT) and DOMAINS REARRANGED METHYLTRANSFERASE (DRM) proteins; however its roles remain poorly understood. Here we characterize the roles of non-CG methylation in Arabidopsis thaliana. We show that a poorly characterized methyltransferase, CMT2, is a functional methyltransferase in vitro and in vivo. CMT2 preferentially binds histone H3 lysine 9 (H3K9) dimethylation and methylates non-CG cytosines that are regulated by H3K9 methylation. We revealed the contributions and redundancies between each non-CG methyltransferase in DNA methylation patterning and in regulating transcription. We also demonstrate extensive dependencies of small RNA accumulation and H3K9 methylation patterning on non-CG methylation, suggesting self-reinforcing mechanisms between these epigenetic factors. The results suggest that non-CG methylation patterns are critical in shaping the histone modification and small non-coding RNA landscapes.

Accumulation of dry mass and macronutrients by sourgrass plants

The experiment was carried out aiming to analyze the dry mass production and distribution and the content and accumulation of macronutrients in sourgrass (Digitaria insularis) plants cultivated under mineral nutrition standard conditions. Plants grew in 7-liter pots filled with sand substrate and daily irrigated with nutrient solution, being maintained under greenhouse conditions. Treatments consisted of times of evaluation (21, 35, 49, 63, 77, 91, 105, 119, and 133 days after emergence - DAE) and were arranged in a completely randomized design with four replicates. Sourgrass showed small accumulation of dry mass (0.3 g per plant) and macronutrients (3.7 mg of N per plant, 0.4 mg of P per plant, 5.6 mg of K per plant, 0.9 mg of Ca per plant, 0.7 mg of Mg per plant, and 0.3 mg of S per plant) at vegetative growth stage (< 49 DAE). Those accumulations increased mainly after 77 DAE, reaching the maximum theoretical value at 143, 135, 141, 129, 125, 120, and 128 DAE, for dry mass (12.4 g per plant), N (163.2 mg per plant), P (27.1 mg per plant), K (260.5 mg per plant), Ca (47.6 mg per plant), Mg (30.9 mg per plant), and S (13.7 mg per plant), respectively. K and N were found with higher rates and, as a consequence, they were required and accumulated in greater amounts in plant tissues of sourgrass.

Biopores and root features as new tools for improving paleoecological understanding of terrestrial sediment-paleosol sequences

The important role of roots and rhizosphere processes is accepted for the top soil, but still under debate for the deep subsoil including soil parent material. Especially for terrestrial sediments like loess and dune sands, roots and root traces are mostly recognized in profile descriptions, but not interpreted in the paleoenvironmental context. Further, synchrony of sediment deposition and root trace formation is commonly assumed. This is challenged by partially large maximum rooting depths of plants, exceeding the soil depth, and by frequent occurrence of secondary carbonates and biopores of potential root origin below recent soil and paleosols. To improve understanding of paleoenvironmental records in terrestrial sediment-paleosol sequences, recent roots and root traces, including calcified roots and root-derived biopores, were investigated in six soil, loess and dune sand profiles across Central and SE Europe. Visualization of small carbonate accumulations (diameter≤1mm), frequently called ‘pseudomycelia’, by X-ray microtomographic scanning, and morphologic comparison with rhizoliths (calcified roots; diameter mostly 3–20mm, up to 100mm possible) indicate root origin of the former, therefore requiring renaming to microrhizoliths. Quantification of roots, biopores, rhizoliths and microrhizoliths on horizontal levels yielded maximum frequencies of 2100m−2, 4100m−2, 196m−2 and 12,800m−2, respectively. Considering the pore volume remaining from former root growth this indicates their significant contribution to structural properties of the sediments and paleosols. Depth distribution of roots and root traces was frequently related to soil and paleosols, respectively, and mostly showed maximum frequencies within or immediately below these units. Root traces are therefore not necessarily of similar age like the surrounding sediment, but are typically of younger age. The time lag between root traces and the surrounding stratigraphic unit can vary between small time periods (likely decades to centuries) in case of microrhizoliths and several millenia in case of larger rhizoliths penetrating several stratigraphic units. With assumed radii of former rhizosphere extension of 5mm for microrhizoliths, a frequency of 12,500m−2 corresponded to 100% rhizosphere area in the respective depth interval. These findings emphasize the meaning of root traces in sediment–paleosol sequences. Potential temporal and spatial inhomogeneity of root growth on the one hand, especially for shrub and tree vegetation, and occurrence of root remains of different age and origin in identical depth intervals on the other hand, hamper the assessment of the chronologic context of these with the surrounding sediment or paleosol. Nevertheless, root traces in terrestrial archives provide valuable information with respect to paleovegetation and paleoenvironmental conditions, if their chronological context is known.

Stratigraphic position and natural environment of the oldest Middle Palaeolithic in central Podolia, Ukraine: New data from the Velykyi Glybochok site

Velykyi Glybochok is an important multi-cultural Palaeolithic site, known since 1979. Besides the Late Palaeolithic (I) and Upper Palaeolithic (II) cultural layers, in this site the following Middle Palaeolithic layers are found: III-B in the soil from the last interglacial, III-A in the Ternopil soil horizon from the penultimate glaciation, and III (the oldest Palaeolithic settlement in Podolia) in the soil from the penultimate interglacial. The ages of these cultural layers are determined from their situation in a complex sequence of loesses and paleosols. In this paper we report the results of excavations carried out in the years 2007–2012, which were supplemented with geophysical recognition. The excavations were conducted in a karst depression formed in Neogene limestones. It is filled and covered by loess containing partially redeposited paleosols. These deposits were sampled for lithological, palaeopedological, palaeomagnetic and palynological analyses, the results of which were used for palaeoenvironmental interpretation. Chronostratigraphy was determined based on TL and IRSL dating as well as palaeomagnetic studies. In comparison with the older investigations, the new discovery is quite well-preserved, with a bipartite complex of paleosols (S2-II and S2-I) from the penultimate interglacial (MIS 7), which occurs between the Odranian (Dnieper) loess L3 and the Wartanian (Tyasmin) loess L2. The paleosols are of interglacial nature. Forest and then steppe vegetation grew during two stages of their formation, which were separated by a cool period when a small accumulation of loess and frost processes occurred. The oldest Middle Palaeolithic cultural layer occurs in the older soil (S2-II), indicating that it is the trace of the oldest Palaeolithic settlement in the western Ukraine. Flint artefacts were made using the Levallois method, rarely found in the sites with the Middle Palaeolithic oldest assemblages, rare in Eastern Europe.

Successful non-operative management of left atrioesophageal fistula following catheter ablation

Atrioesophageal fistula (AEF) is a potentially lethal complication of catheter radiofrequency ablation for atrial fibrillation. A 49-year-old man with paroxysmal atrial fibrillation who underwent catheter ablation around the pulmonary vein was admitted 31days after the procedure, suffering seizures and fever. Magnetic resonance imaging of the brain showed ischemia and multiple lesions of acute infarction in the right occipital lobe of the cerebrum. Computed tomography (CT) of the chest showed a small accumulation of air between the posterior left atrium and the esophagus, suggesting an AEF. Endoscopic snaring of the esophageal mucosa, repeated a few times, supported by nil by mouth and antibiotic therapy, resulted in improvement of his condition with no recurrence of symptoms. Subsequent chest CT scans confirmed disappearance of the leaked air and the patient was discharged home 45days after admission with no neurological compromise.

A novel acoustic imaging tool for monitoring the state of rapid sand filters

A new technology based on acoustic waves is developed to monitor the state of sand filters used in drinking water treatment. Changes in the sand filter, due to the removal of suspended particles from the water and their accumulation in the pores, result in an increase of the bulk density and acoustic speed of the granular material. Consequently, the reflected acoustic response changes as the filter is in use. To monitor these changes, an instrument composed of an omnidirectional transmitter and an array of hydrophones was built. With frequencies ranging between 10 and 110 kHz, high resolution is achieved in the vertical direction enabling the detectability of clogged layers with a minimum thickness of 1 cm. The novel instrument is tested by conducting a monitoring experiment in a filter used in practice. A 2D scan over a part of the filter was performed and repeated every 2 hours over a period of 10 days. An analysis of the data revealed a local increase of the reflected acoustic response with increasing filter run time. The changes in acoustic signal are mainly observed at the upper 5 cm of the sand bed. It is also clear that the filter bed is slowly compacting as a function of time. The total compaction after a period of 10 days reached 3.5 cm. The filter bed is expanded again during the cleaning procedure. Once the procedure is completed, the upper 30 cm of the filter becomes more transparent, showing small accumulations of material at greater depth. The observed changes in the filter bed demonstrate the potential of this acoustic-based tool to monitor the state of rapid sand filters and optimise their performance. The new tool can be used to evaluate the cleaning procedure and is valuable in detecting lateral variations in the filter bed. These variations may indicate local clogging that needs to be removed effectively to avoid deterioration of the overall performance in the long term. This type of information is difficult to obtain from the monitoring techniques currently used in drinking water treatment.

Atypical and Classical Forms of the Disease-Associated State of the Prion Protein Exhibit Distinct Neuronal Tropism, Deposition Patterns, and Lesion Profiles

A number of disease-associated PrP forms characterized by abnormally short proteinase K-resistant fragments (atypical PrPres) were recently described in prion diseases. The relationship between atypical PrPres and PrP(Sc), and their role in etiology of prion diseases, remains unknown. We examined the relationship between PrP(Sc) and atypical PrPres, a form characterized by short C-terminal proteinase K-resistant fragments, in a prion strain of synthetic origin. We found that the two forms exhibit distinct neuronal tropism, deposition patterns, and degree of pathological lesions. Immunostaining of brain regions demonstrated a partial overlap in anatomic involvement of the two forms and revealed the sites of their selective deposition. The experiments on amplification in vitro suggested that distinct neuronal tropism is attributed to differences in replication requirements, such as preferences for different cellular cofactors and PrP(C) glycoforms. Remarkably, deposition of atypical PrPres alone was not associated with notable pathological lesions, suggesting that it was not neurotoxic, but yet transmissible. Unlike PrP(Sc), atypical PrPres did not show significant perineuronal, vascular, or perivascular immunoreactivity. However, both forms showed substantial synaptic immunoreactivity. Considering that atypical PrPres is not associated with substantial lesions, this result suggests that not all synaptic disease-related PrP states are neurotoxic. The current work provides important new insight into our understanding of the structure-pathogenicity relationships of transmissible PrP states.

Surface modification of PLGA nanoparticles via human serum albumin conjugation for controlled delivery of docetaxel

BackgroundPoly lactic-co-glycolic acid (PLGA) based nanoparticles are considered to be a promising drug carrier in tumor targeting but suffer from the high level of opsonization by reticuloendothelial system due to their hydrophobic structure. As a result surface modification of these nanoparticles has been widely studied as an essential step in their development. Among various surface modifications, human serum albumin (HSA) possesses advantages including small size, hydrophilic surface and accumulation in leaky vasculature of tumors through passive targeting and a probable active transport into tumor tissues.MethodsPLGA nanoparticles of docetaxel were prepared by emulsification evaporation method and were surface conjugated with human serum albumin. Fourier transform infrared spectrum was used to confirm the conjugation reaction where nuclear magnetic resonance was utilized for conjugation ratio determination. In addition, transmission electron microscopy showed two different contrast media in conjugated nanoparticles. Furthermore, cytotoxicity of free docetaxel, unconjugated and conjugated PLGA nanoparticles was studied in HepG2 cells.ResultsSize, zeta potential and drug loading of PLGA nanoparticles were about 199 nm, −11.07 mV, and 4%, respectively where size, zeta potential and drug loading of conjugated nanoparticles were found to be 204 nm, −5.6 mV and 3.6% respectively. Conjugated nanoparticles represented a three-phasic release pattern with a 20% burst effect for docetaxel on the first day. Cytotoxicity experiment showed that the IC50 of HSA conjugated PLGA nanoparticles (5.4 μg) was significantly lower than both free docetaxel (20.2 μg) and unconjugated PLGA nanoparticles (6.2 μg).ConclusionIn conclusion surface modification of PLGA nanoparticles through HSA conjugation results in more cytotoxicity against tumor cell lines compared with free docetaxel and unconjugated PLGA nanoparticles. Albumin conjugated PLGA nanoparticles may represent a promising drug delivery system in cancer therapy.

The transition from differentiation to growth during dermomyotome-derived myogenesis depends on temporally restricted hedgehog signaling

The development of a functional tissue requires coordination of the amplification of progenitors and their differentiation into specific cell types. The molecular basis for this coordination during myotome ontogeny is not well understood. Dermomytome progenitors that colonize the myotome first acquire myocyte identity and subsequently proliferate as Pax7-expressing progenitors before undergoing terminal differentiation. We show that the dynamics of sonic hedgehog (Shh) signaling is crucial for this transition in both avian and mouse embryos. Initially, Shh ligand emanating from notochord/floor plate reaches the dermomyotome, where it both maintains the proliferation of dermomyotome cells and promotes myogenic differentiation of progenitors that colonized the myotome. Interfering with Shh signaling at this stage produces small myotomes and accumulation of Pax7-expressing progenitors. An in vivo reporter of Shh activity combined with mouse genetics revealed the existence of both activator and repressor Shh activities operating on distinct subsets of cells during the epaxial myotomal maturation. In contrast to observations in mice, in avians Shh promotes the differentiation of both epaxial and hypaxial myotome domains. Subsequently, myogenic progenitors become refractory to Shh; this is likely to occur at the level of, or upstream of, smoothened signaling. The end of responsiveness to Shh coincides with, and is thus likely to enable, the transition into the growth phase of the myotome.

MODELLING HYDROCARBON GENERATION IN THE PALAEOZOIC AND MESOZOIC SUCCESSIONS IN SE POLAND AND WEST UKRAINE

The Lower Palaeozoic succession in SE Poland and West Ukraine has source rock potential, particularly the Ordovician and Silurian which contain oil‐prone Type II kerogen. The thermal maturity of these units ranges from early to late‐phase oil window (locally up to gas window). Within the Mesozoic succession, source rock potential is highest in the Middle Jurassic which has TOC of up to 26 wt% and a genetic potential of up to 39 mg/g of rock, with organic matter dominated by gas‐prone Type III kerogen. In SE Poland, the organic matter in this unit is generally immature, whereas maturities in West Ukraine are sufficient for hydrocarbon generation to occur.Modelling of hydrocarbon generation suggested that petroleum in Lower Palaeozoic source rocks began to be generated in the Early Carboniferous. Peak generation took place from the late Visean to the early Namurian, and terminated either as a result of source rock depletion or Variscian inversion. Expelled hydrocarbons migrated during post‐Carboniferous and Mesozoic uplift. Middle Jurassic source rocks in SE Poland have only reached the early oil window. Higher thermal maturities in the Ukraine resulted in hydrocarbon generation and expulsion. This took place after Miocene burial and maturation.A number of small hydrocarbon accumulations occur in Mesozoic reservoirs in SE Poland / West Ukraine, and hydrocarbons have migrated from Cambrian and Ordovician source rocks. However, the prospectivity of the study area is reduced as a result of phases of uplift and intense erosion which allowed hydrocarbons to escape from structural traps.

Treatment of pyloric stenosis in a cat via pylorectomy and gastroduodenostomy (Billroth I procedure)

A 5-month-old 1.9-kg (4.2-lb) spayed female Siamese cat was evaluated because of a history of decreased appetite, regurgitation, vomiting, and lack of weight gain. Radiographic findings included a fluid- and gas-distended stomach with a small accumulation of mineral opacities. Ultrasonographic examination confirmed severe fluid distention of the stomach with multiple hyperechoic structures present and revealed protrusion of the thickened pylorus into the gastric lumen, with normal pylorogastric serosal continuity. Endoscopy of the upper gastrointestinal tract revealed an abnormally shortened pyloric antrum and stenotic pyloric outflow orifice. Pyloric stenosis resulting in pyloric outflow obstruction was diagnosed. A pylorectomy with end-to-end gastroduodenostomy (Billroth I procedure) was successfully performed, and a temporary gastrostomy tube was placed. Six days after surgery, the cat was eating and drinking normally, with the tube only used for administration of medications. The gastrostomy tube was removed 12 days after surgery. Results of follow-up examination by the referring veterinarian 3 weeks after surgery were normal. Occasional vomiting approximately 2 months after surgery was managed medically. Fifteen months after surgery, the owners reported that the cat seemed completely normal in appearance and behavior. Pyloric stenosis should be considered a differential diagnosis for young cats with pyloric outflow obstruction. The cat of this report was treated successfully with a Billroth I procedure. Histologic examination and immunohistochemical analysis of the excised tissue showed the stenosis to be associated with hypertrophy of the tunica muscularis.

Meteorological regimes and accumulation patterns at Utsteinen, Dronning Maud Land, East Antarctica: Analysis of two contrasting years

Since February 2009, an automatic weather station (AWS) has been operating near Utsteinen Nunatak, north of the Sør Rondane Mountains, in Dronning Maud Land at the ascent to the East Antarctic Plateau. This paper gives an assessment of the meteorological conditions, radiative fluxes, and snow accumulation for the first 2 years of operation, 2009 to 2010, analyzed in terms of meteorological regimes. Three major meteorological regimes—cold katabatic, warm synoptic, and transitional synoptic—are identified using cluster analysis based on five parameters derived from the AWS measurements (wind speed, specific humidity, near‐surface temperature inversion, surface pressure, and incoming longwave flux indicative of cloud forcing). For its location, the relatively mild climate at Utsteinen can be explained by the high frequency of synoptic events (observed 41%–48% of the time), and a lack of drainage of cold air from the plateau due to mountain sheltering. During the cold katabatic regime, a strong surface cooling leads to a strong near‐surface temperature inversion buildup. A large difference in accumulation is recorded by the AWS for the first 2 years: 235 mm water equivalent in 2009 and 27 mm water equivalent in 2010. Several large accumulation events during the warm synoptic regime occurring mainly in winter were responsible for the majority of the accumulation in 2009. Mostly, small accumulation events occurred during 2010, frequently followed by snow removal. This interannual variability in snow accumulation at the site is related to the intensity of the local synoptic events as recorded by meteorological regime characteristics.

Competitive capacity of cassava with weeds: Implications on accumulation of dry matter

This work aimed to determine the competitive ability of two varieties of cassava against six weed species at initial growth stages, in relation to the allocation of dry mass in plants. The trial was conducted as a factorial experiment, with two cassava genotypes (IAC - 12 and Periquita) under competition with six weed species (Euphorbia heterophylla, Bidens pilosa, Cenchrus echinatus, Amaranthus spinosus, Commelina benghalensisand Brachiaria plantaginea), plus eight treatments corresponding to cassava varieties and weed species free from competition. The period of competition between cassava varieties and weeds was 75 days after crop emergence, when shoot and root mass were collected for evaluation of leaf area as well as, dry mass accumulation and distribution along plant organs (roots, leaves and stem). Cassava varieties presented smaller dry mass accumulation when under competition with weed species. Roots were the most affected organ by the competition. On the other hand, partition of dry mass in weeds was barely affected. In general terms, cassava variety Periquita was the most tolerant genotype to the competition and B. plantaginea was the weed species with higher competitive ability. Key words: Weed species, cassava genotypes, competition, dry matter accumulation.

Influence of Human OATP1B1, OATP1B3, and OATP1A2 on the Pharmacokinetics of Methotrexate and Paclitaxel in Humanized Transgenic Mice

Organic anion-transporting polypeptide (OATP) drug uptake transporters are thought to play an important role in drug pharmacokinetics and toxicokinetics. We aimed to determine the influence of the individual human OATP1B1, OATP1B3, and OATP1A2 transporters on the in vivo disposition of the anticancer drugs methotrexate and paclitaxel by using liver-specific humanized OATP1A/1B transgenic mice. Wild-type, Slco1a/1b(-/-) (Oatp1a/1b knockout), Slco1a/1b(-/-);1B1(tg), Slco1a/1b(-/-);1B3(tg), and newly generated Slco1a/1b(-/-);1A2(tg) (humanized OATP1B1, OATP1B3, and OATP1A2 transgenic) mice were characterized biochemically and physiologically, and subsequently intravenously dosed with methotrexate or paclitaxel (2 or 10 mg/kg each) for pharmacokinetic analyses. Humanized OATP1B1, OATP1B3, and OATP1A2 transgenic mice all showed partial or complete rescue of increased plasma bilirubin levels, but also of the increased plasma levels and decreased liver and small intestinal accumulation of methotrexate observed in Slco1a/1b(-/-) mice. Furthermore, hepatic expression of OATP1B3 and OATP1A2, but not OATP1B1, resulted in increased liver uptake of paclitaxel (2 mg/kg). At 10 mg/kg, a modest effect of only OATP1A2 on paclitaxel liver uptake was observed. Human OATP1A/1B transporters play an important role in plasma and tissue distribution of the structurally diverse chemotherapeutics methotrexate (organic anion) and paclitaxel (hydrophobic, bulky). Variation in OATP1A/1B activity due to genetic variation and pharmacologic inhibition, or differences in tumor-specific expression levels might therefore affect plasma, tissue, and tumor levels of these drugs in patients, and hence their therapeutic efficacy. Humanized transgenic OATP1A/1B mice will provide excellent tools to further study these aspects in vivo for many (anticancer) drugs.

Dissolved methane in Indian freshwater reservoirs

Emission of methane (CH4), a potent greenhouse gas, from tropical reservoirs is of interest because such reservoirs experience conducive conditions for CH4 production through anaerobic microbial activities. It has been suggested that Indian reservoirs have the potential to emit as much as 33.5MT of CH4 per annum to the atmosphere. However, this estimate is based on assumptions rather than actual measurements. We present here the first data on dissolved CH4 concentrations from eight freshwater reservoirs in India, most of which experience seasonal anaerobic conditions and CH4 buildup in the hypolimnia. However, strong stratification prevents the CH4-rich subsurface layers to ventilate CH4 directly to the atmosphere, and surface water CH4 concentrations in these reservoirs are generally quite low (0.0028-0.305μM). Moreover, only in two small reservoirs substantial CH4 accumulation occurred at depths shallower than the level where water is used for power generation and irrigation, and in the only case where measurements were made in the outflowing water, CH4 concentrations were quite low. In conjunction with short periods of CH4 accumulation and generally lower concentrations than previously assumed, our study implies that CH4 emission from Indian reservoirs has been greatly overestimated.

Quantitative Comparison of PET and Bremsstrahlung SPECT for Imaging the In Vivo Yttrium-90 Microsphere Distribution after Liver Radioembolization

BackgroundAfter yttrium-90 (90Y) microsphere radioembolization (RE), evaluation of extrahepatic activity and liver dosimetry is typically performed on 90Y Bremsstrahlung SPECT images. Since these images demonstrate a low quantitative accuracy, 90Y PET has been suggested as an alternative. The aim of this study is to quantitatively compare SPECT and state-of-the-art PET on the ability to detect small accumulations of 90Y and on the accuracy of liver dosimetry.Methodology/Principal FindingsSPECT/CT and PET/CT phantom data were acquired using several acquisition and reconstruction protocols, including resolution recovery and Time-Of-Flight (TOF) PET. Image contrast and noise were compared using a torso-shaped phantom containing six hot spheres of various sizes. The ability to detect extra- and intrahepatic accumulations of activity was tested by quantitative evaluation of the visibility and unique detectability of the phantom hot spheres. Image-based dose estimates of the phantom were compared to the true dose. For clinical illustration, the SPECT and PET-based estimated liver dose distributions of five RE patients were compared. At equal noise level, PET showed higher contrast recovery coefficients than SPECT. The highest contrast recovery coefficients were obtained with TOF PET reconstruction including resolution recovery. All six spheres were consistently visible on SPECT and PET images, but PET was able to uniquely detect smaller spheres than SPECT. TOF PET-based estimates of the dose in the phantom spheres were more accurate than SPECT-based dose estimates, with underestimations ranging from 45% (10-mm sphere) to 11% (37-mm sphere) for PET, and 75% to 58% for SPECT, respectively. The differences between TOF PET and SPECT dose-estimates were supported by the patient data.Conclusions/SignificanceIn this study we quantitatively demonstrated that the image quality of state-of-the-art PET is superior over Bremsstrahlung SPECT for the assessment of the 90Y microsphere distribution after radioembolization.