Slow-release Formulation Research Articles

Use of a Capsule Suspension Formulation of S-metolachlor in Fenclorim-treated Rice

Abstract As herbicide resistance continues to render commonly used rice herbicides ineffective, alternative sites of action are paramount to maintaining yield and producer profitability. Combining a slow-release formulation and a fenclorim seed treatment might allow the safe use of S-metolachlor in rice. Experiments were initiated in 2022 and 2023 near Colt, AR, on a silt loam soil to evaluate crop safety using a capsule suspension (CS) formulation of S-metolachlor and a fenclorim seed treatment in rice. The first experiment assessed the tolerance of two cultivars (Diamond and DG263L) to three rates (0.42, 0.84, 1.68 kg ai ha-1) of a CS S-metolachlor at a delayed preemergence (DPRE) application timing in conjunction with a fenclorim seed treatment. The second experiment evaluated a 1- to 2-leaf (EPOST) application of a CS S-metolachlor at 0.56 and 1.12 kg ai ha-1 to fenclorim-treated rice. Fenclorim reduced injury and partially protected rice yield when S-metolachlor was applied DPRE at 1.68 kg ai ha-1 in both years. However, in one year under adverse conditions, rice yields were only 65% and 66% of the nontreated control for fenclorim-treated Diamond and DG263L, respectively. An EPOST application of S-metolachlor at 1.12 kg ai ha-1 resulted in 44 to 51% visible injury 35 d after treatment. Relative rice yields were 88% and 89% of the nontreated weed-free treatment in 2022 and 2023, respectively. Fenclorim provided enhanced crop safety at both the 0.84 and 1.68 kg ai ha-1 rates of S-metolachlor. However, the potential for reduced yield can occur when unfavorable conditions occur soon after application. An EPOST application timing of CS S-metolachlor at 0.56 kg ai ha-1 may be a viable option in rice, but 1.12 kg ai ha-1 is too high on a silt loam soil, resulting in significant rice injury.

Effect of a Slow-Release Urea Nanofertilizer on Soil Microflora and Yield of Direct Seeded Rice (Oryza sativa L.)

Nitrogen fertilizers have a significant impact on the growth of rice. The overuse and inappropriate application of nitrogen fertilizers have resulted in environmental pollution, in addition to subjecting both humans and livestock to negative health hazards. Finding a viable substitute for traditional nitrogen fertilizers is crucial and essential to help improve crop yield and minimize environmental damage. Nano-nitrogen fertilizers offer a possible alternative to traditional fertilizers due to a slow/controlled release of nitrogen. The present work aimed to study the effect of a slow-release urea nanofertilizer on soil ammonical (NH4-N) and nitrate-N (NO3-N) content, culturable soil microflora, and soil enzyme activities in three different soil samples procured from Ludhiana and Patiala districts through a soil column study. Seven treatments, including 0, 50 (75 kg/ha N), 75 (112.5 kg/ha N), and 100% (150 kg/ha N) of the recommended dose (RD) of conventional urea and nano-urea fertilizer were applied. The leachate samples collected from nano-urea treatment exhibited NH4-N for the first two weeks, followed by NO3-N appearance. The higher NH4-N and NO3-N contents in the leachate were recorded for light-textured soil as compared to medium- and heavy-textured soil samples. The soil microbial counts and enzyme activities were recorded to be maximum in light-textured soils. Therefore, this slow-release formulation could be more useful for light-textured soils to decrease applied N-fertilizer losses, as well as for improving the soil microbial viable cell counts and soil enzyme activities. The effect of urea nanofertilizer on the growth and yield of direct-seeded rice (Oryza sativa L.) was also evaluated under field conditions. Both studies were performed independently. Numerically, the highest shoot height, fresh and dry shoot weight, and significantly maximum total chlorophyll, carotenoid, and anthocyanins were recorded in the T2 (100% RDF through nano-urea) treatment. The yield-attributing traits, including the number of filled grains and thousand-grain weight, were also recorded to have increased in T2 treatment. A numerical increase in NPK for plant and grain of rice at 100% RDN through nano-urea was recorded. The soil application of the product exhibited no negative effect on the soil microbial viable cell count on different doses of nano-urea fertilizer. The soil nitrogen fixer viable counts were rather improved in nano-urea treatments. The results reflect that nano-urea fertilizer could be considered as a possible alternative to conventional fertilizer.

Slow Subcutaneous Release of Glatiramer Acetate or CD40-Targeting Peptide KGYY6 Is More Advantageous in Treating Ongoing Experimental Autoimmune Encephalomyelitis.

One of the first-line disease-modifying treatments of multiple sclerosis (MS) is Glatiramer Acetate (GA), which requires daily or three-times-weekly subcutaneous injections. Disease progression, while slowed, still occurs with time. Increasing the impact of the treatment while decreasing the frequency of injections would be ideal. The mechanism of action of GA remains undefined. We developed an alternate approach, KGYY6, whose mechanism of action targets the CD40 receptor with promising results in an Experimental Autoimmune Encephalomyelitis (EAE) model. GA and a CD40-targeting peptide, KGYY6, were formulated as slow-release particles used to treat EAE in C57BL/6 mice. Compared to liquid formulations, the particle formulations vastly improved drug efficacy in both cases, which would be advantageous in treating MS. GA is a combination of randomly generated peptides, in the size range of 5000-9000 Da, using the amino acids E, A, Y, and K. This approach introduces batch differences that impacts efficacy, a persistent problem with GA. KGYY6 is generated in a controlled process and has a motif, K-YY, which could be generated when manufacturing GA. When testing two different lots of GA or KGYY6, the latter performed equally well across lots, while GA did not. Slow-release formulations of both GA and KGYY6 vastly improve the efficacy of both, and KGYY6 is more consistent in efficacy across different lots.

Greener Production and Application of Slow-Release Nitrogen Fertilizer Using Plasma and Nanotechnology: A Review

The potential of both plasma and nanotechnology in producing slow-release fertilizer is immense. These technologies, when combined, may offer green and inexpensive nitrogen fertilizers, from rich renewable resources available in local areas. Together, these technologies may overcome some limitations of conventional synthetic fertilizers, which are currently expensive and associated with low nitrogen use efficiency and significant environmental concerns. This review explores the utilization of recent advances in plasma and nanotechnology, which can be leveraged to create new slow-release nitrogen fertilizers. It emphasizes their crucial role in addressing nitrogen depletion and improving crop production. Despite the lack of attempts to develop slow-release nanofertilizers from low-cost liquid nitrate generated by emission-free nonthermal plasma, the effectiveness of plasma nitrate matches that of conventional fertilizer for crop production. We propose a more efficient electrocatalytic conversion of plasma nitrate to ammonium salt, then coating it with plant-based cellulose nanoparticles to create a slow-release form. This set of processes would synchronize nutrient release with the dynamic N requirements of plants. Formulations using agro-based, low-cost cellulose nanomaterials could replace high-cost carrier hydrogels associated with low mechanical strength. This review also highlights the isolation of nanocellulose from various plant materials and its characterization in different formulations of slow-release nanoplasma N fertilizer. Additionally, we discuss mechanisms of N loss, slow-release, and retention in the soil that can contribute to the production and use of efficient, sustainable fertilizers to improve food security and, consequently, the health of our planet.

Increased incidence of weight-loss-associated humane endpoints in rats administered buprenorphine slow-release LAB formulation following traumatic brain injury: a retrospective study.

Traumatic brain injury (TBI) remains a significant global public health epidemic with adverse health and cost implications. Due to its complex, heterogeneous nature and wide-ranging impacts, definitive TBI treatments remain elusive. As such, continued laboratory research using animal models is warranted. In accordance with guidelines set forth for the humane treatment of research animals, TBI animal models are often administered analgesics for pain management. The choice of drug, timing, dose, and formulation of analgesic can vary depending on the study's unique needs and can potentially and unintentionally influence experimental results. In TBI studies utilizing rats as animal models, buprenorphine is a common analgesic administered. In addition to pain management in such studies, investigators must also monitor the research animals post-operatively and make the decision for humane euthanasia before intended experimental survival timepoint if the animals are assessed to be excessively suffering. This study investigated the differences in adult, male Sprague Dawley rats used for various TBI studies that reached weight-loss-induced humane endpoints following a single administration of buprenorphine slow-release LAB (bup-SR-LAB) or buprenorphine slow-release HCl (bup-SR-HCl). Our findings indicate that TBI-induced rats receiving bup-SR-LAB in conjunction with a secondary surgical insult such as artificial intracranial pressure elevation and/or osmotic pump implantation reach a weight-loss-induced humane euthanasia endpoint more often compared to sham-injured rats. When stratifying into the same groups, we did not find this pattern to hold true for rats administered bup-SR-HCl. Overall, this study contributes to the limited body of literature addressing different analgesic formulations' effects on laboratory animals.

Persistent opioid use after hospital admission due to trauma: a population-based cohort study.

Persistent opioid use (POU) is a common marker of harm related to opioid use after trauma. This study determined the incidence and risk factors for POU after hospitalisation due to trauma in New Zealand, among opioid-naïve patients. This was a population-based, retrospective cohort study, using linked data, involving all trauma patients of any age admitted to all NZ hospitals between 2007 and 2019. We included all patients who received opioids after discharge and were considered opioid naïve, defined as not having received opioids or not having a prior diagnosis of opioid-use disorder up to 365 days preceding the discharge date. The primary outcome was the incidence of POU defined as opioid use after discharge between 91 and 365 days. We used a multivariable logistic regression to identify independent risk factors for POU. A total of 177,200 patients were included in this study. Of these, 15.3% (n = 27,060) developed POU based on criteria used for the primary analysis, with sensitivity analyses showing POU incidence ranging from 14.3% to 0.8%. The opioid exposure risk factors associated with POU included switching between different opioids (adjusted odds ratio [aOR] 2.62; 95% confidence interval [CI] 2.51-2.73), prescribed multiple opioids (vs codeine, aOR 1.44; 95% CI 1.37-1.53), slow-release opioid formulations (aOR 1.32; 95% CI 1.26-1.39), and dispensed higher total doses of on the initial discharge prescription (aOR 1.26; 95% CI 1.20-1.33). Overall, 1 in 7 opioid-naïve patients who were exposed to opioids after trauma developed POU. Our findings highlight clinicians should be aware of these factors when continuing opioids on discharge.

Chitosan-coated liposomal systems for delivery of antibacterial peptide LL17-32 to Porphyromonas gingivalis

Periodontal disease is triggered by surface bacterial biofilms where bacteria are less susceptible to antibiotic treatment. The development of liposome-based delivery mechanisms for the therapeutic use of antimicrobial peptides is an attractive alternative in this regard. The cationic antimicrobial peptide LL-37 (human cathelicidin) is well-known to exert antibacterial activity against Porphyromonasgingivalis, a keystone oral pathogen. However, the antibacterial activity of the 16-amino acid fragment (LL17-32) of LL-37, is unknown. In addition, there are still gaps in studies using liposomal formulations as delivery vehicles of antibacterial peptides against this pathogen. This study was designed to examine the influence of the different types of liposomal formulations to associate and deliver LL17-32 to act against P. gingivalis. Chitosans of varying Mw and degree of acetylation (DA) were adsorbed at the surface of soya lecithin (SL) liposomes. Their bulk (average hydrodynamic size, ζ-potential and membrane fluidity) and ultrastructural (d-spacing, half-bilayer thickness and the water layer thickness) biophysical properties were investigated by a panel of techniques (DLS, SAXS, M3-PALS, fluorescence spectroscopy and TEM imaging). Their association efficiency, in vitro release, stability, and efficacy in killing the periodontal pathogen P. gingivalis were also investigated. All liposomal systems possessed spherical morphologies and good shelf-life stabilities. Under physiological conditions, chitosan formulations with a high DA demonstrated enhanced stability in comparison to low DA-chitosan formulations. Chitosans and LL17-32 both decreased SL-liposomal membrane fluidity. LL17-32 exhibited a high degree of association with SL-liposomes without in vitro release. In biological studies, free LL17-32 or chitosans alone, demonstrated microbicidal activity against P. gingivalis, however this was attenuated when LL17-32 was loaded onto the SL-liposome delivery system, presumably due to the restrained release of the peptide. A property that could be harnessed in future studies (e.g., oral mucoadhesive slow-release formulations).

Valorization of lignin by crosslinking for slow-release urea fertilizer systems: A promising approach for agricultural applications

Slow-release fertilizers hold promise for their extensive use in agriculture. Urea, a commonly employed fertilizer, poses the risk of elevated nitrogen levels in soil due to its water solubility when used improperly. To address this issue, the development of a cost-effective and biodegradable urea slow-release matrix was pursued by crosslinking wheat straw-derived lignin with epichlorohydrin at varying ratios (1:1, 1:2, and 2:1) and subsequently impregnating it with urea. The crosslinked lignin matrices exhibited superior properties compared to unmodified lignin. After 8 days of immersion in water, the percentage release of urea from the crosslinked lignin matrices reached 48%, whereas it was 95% for unmodified lignin. Characterization techniques such as Fourier transform infrared spectroscopy, 13C nuclear magnetic resonance, and thermogravimetry analysis were employed to gain insights into the structural and functional aspects of the crosslinked lignin. The results revealed that the crosslinked lignin possessed a higher surface area and exhibited more suitable functional groups for urea impregnation. The controlled hydrophilic-lipophilic balance of the crosslinked lignin matrices offers significant advantages in fertilizer systems, enabling a controlled and gradual release of urea. This innovative approach not only offers enhanced control over nutrient delivery but also demonstrates the potential of lignin as a sustainable and cost-effective alternative for slow-release fertilizer formulations. This development provides an effective solution to address the issue of excessive nitrogen levels and opens up new avenues in material development, holds promise for sustainable agriculture and paves the way for further advancements in the field.

The effect of twice daily 3-nitroxypropanol supplementation on enteric methane emissions in grazing dairy cows

Although 3-nitroxypropanol (3-NOP) has been proven to reduce enteric methane (CH4) by ∼30% in indoor systems of dairying when the additive is mixed throughout a TMR, very limited research has been done to date in grazing systems in which the most convenient method of additive supplementation is at milking twice daily. To investigate the effect of twice daily 3-NOP supplementation on enteric CH4 emissions, a 12-wk study was undertaken in which treatment cows (n = 26) were supplemented with 3-NOP (80 mg/kg DMI) twice daily at morning and evening milking, and control cows (n = 26) received no additive supplementation. Enteric CH4, hydrogen (H2) and carbon dioxide (CO2) were measured using GreenFeed units, and milk production, BW, BCS, and DMI were monitored to determine the effect of 3-NOP supplementation on productivity. No significant effect was observed for 3-NOP supplementation on any of the aforementioned parameters with the exception of CH4 and H2 production, respectively. Cows supplemented with 3-NOP produced 1.6-fold more H2 (P < 0.001) across a 24-h period, with reductions in CH4 production of 28.5% recorded in the 3 h after additive consumption (P < 0.001), however, levels of CH4 production returned to that of the control group thereafter. When CH4 production was considered across the entire 24-h period, the cows offered 3-NOP produced ∼5% less CH4 than the control (P < 0.050). Future research should focus on methods to increase the efficacy of the additive throughout the day which would include the deployment of a slow-release form or an out-of-parlor feeding system that allows animals consume the product at additional time points.

Evaluation of granular formulated strigolactone analogs for Striga suicidal germination.

Striga hermonthica, an obligate root parasitic weed, poses a significant threat to cereal production in sub-Saharan Africa. Lowering Striga seed bank in infested soils is a promising strategy to mitigate infestation levels. The dependency of Striga seed germination on strigolactones opens up the possibility of a 'suicidal germination' approach, where synthetic germination stimulants induce lethal germination in the absence of a host. Implementing this approach requires active germination stimulants with a suitable formulation for field application. Here, we describe the development of slow-releasing granular formulation of two potent germination stimulants 'Methyl Phenlactonoate 3' and 'Nijmegen-1' and the assessment of their activity under Laboratory, greenhouse, mini-field, and field conditions. Under laboratory conditions, the granular formulation of either of the two germination stimulants (1.25 mg per plate, corresponding to 0.09 mg a.i.) induced Striga seed germination at a rate of up to 43%. With 10 mg granular product (0.75 mg a.i.) per pot, we observed 77-83% reduction in Striga emergence under greenhouse pot conditions. Application of the formulated stimulants under artificially or naturally infested fields resulted in approximately 56%, 60%, and 72% reduction in Striga emergence in maize, sorghum, and millet fields in Kenya and Burkina Faso, respectively. Our findings on the newly designed granular formulation of Methyl Phenlactonoate 3 and Nijmegen-1 reveal encouraging prospects for addressing the Striga problem in Africa. These findings underscore several significant advantages of the formulated stimulants, including suitability for the African agricultural context, and, most importantly, their effectiveness in reducing Striga infection. © 2024 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Effect of NPK Bio-Organomineral Tablet Fertilizer on Harvest Index, Wet Weight, Dry Weight and Fertilization Efficiency in the Waste Contaminated Soil

Contamination of rice fields due to industrial activities had an impact on reduced soil fertility, heavy metal contamination from industrial waste, and reduced rice yields. To overcomed this problem, this could be done by applied NPK Bio-organomineral tablet fertilizer. NPK Bio-organomineral tablet fertilizer was a modification of fertilizer made from urea, guano, palm oil empty fruit bunch charcoal, zeolite, activated charcoal, and biologically enhanced compost, turning the fertilizer into a slow-release form. The experiment aimed to investigate the impact of applied NPK Bio organomineral tablet fertilizer on harvest index, wet weight, dry weight and fertilization efficiency in the waste contaminated soil. This experiment was conducted from September 2023 to January 2024 at the Greenhouse Experimental Garden of Ciparanje, Jatinangor, and the Soil Fertility and Plant Nutrition Laboratory, Universitas Padjadjaran. The experimental design used was a Randomized Block Design (RBD) consisted of eight treatments and four replications, included control, NPK Phonska 100% recommendation 250 kg/ha, and 125; 250; 375; 500; 625; 750 kg/ha of NPK Bio-organomineral tablet fertilizer. The results of the experiment indicated that NPK Bio-organomineral tablet fertilizer had an impact on harvest index, wet weight, dry weight and fertilization efficiency in the waste contaminated soil. The optimal dose of NPK Bio-organomineral tablet fertilizer was 375 kg/ha, showed the most significant improvement on harvest index, wet weight, dry weight and fertilization efficiency in the waste contaminated soil.

Avicenna's views on pest control and medicinal plants he prescribed as natural pesticides.

The present study aimed to introduce Avicenna's views on pest control and the medicinal plants he proposed as natural pesticides. Also, we addressed the strategies that he leveraged to formulate and prescribe them, and, finally, we put his views into perspective with modern science. The data were collected using Al-Qanun Fi Al-Tibb (The Canon of Medicine) as well as scientific databases. According to Al-Qanun Fi Al-Tibb, 42medicinal plants are described as natural pest control agents. After introducing the pest control properties of each plant, Avicenna explained the appropriate strategies for use of these plants. These strategies or formulations included incensing, spraying, spreading, rubbing, smudging, and scent-dispersing, which are equivalent to the modern pesticide formulations of fumigants, aerosols, pastes and poisoned baits, lotions, creams, and slow-release formulations, respectively. This study revealed that Avicenna introduced the pest control approach with natural plants in his book Al-Qanun Fi Al-Tibb and, thus, harnessed the power of nature to control nature. Future research is recommended to find the pest control merits of the presented medicinal plants, in order to incorporate them into pest control programs and reduce environmental pollution resulting from the complications of current synthetic pesticides.

Diversity of Providing Services to Patients Suffering from Addiction Disorders is a Harm Reduction Thought, but...

: The diversity of the portfolio of pharmaceutical and non-pharmacological services is an undeniable necessity; however, we must remember that the thinking of harm reduction should govern this process. If we only pay attention to the variety of drugs and their different forms, eventually the noble goal of harm reduction will suffer. In an article that Pedersen et al. prepared about the slow-release form of buprenorphine, there are structural and content problems that will be addressed in this article. This article criticizes the rapid change in the provision of harm reduction services and discusses the impact of structural changes in the provision of services and the location of service provision.

A urea-loaded hydrogel comprising of cellulose nanofibers and carboxymethyl cellulose: An effective slow-release fertilizer

Conventional fertilizers used worldwide to increase crop yield have several environmental and economic issues resulting from their quickly soluble nature, causing most of the fertilizers to be lost through agricultural field runoff and leachate. Utilizing slow or controlled-release fertilizers may reduce the issues associated with conventional fertilizers. However, many commercially available variants of slow-release formulations are ecologically unsustainable as they are made of non-biodegradable polymers. In the present study, a hydrogel composed of cellulose nanofibers (CNF) and carboxyl methyl cellulose (CMC) was synthesized for use as a biodegradable and environmentally sustainable host for carrying urea within its matrix and released the same slowly over a long period of time. The swelling measurements showed that the thermally stable urea-loaded CNF/CMC hydrogel (UCNF) had a high water absorption capacity of 147 g/g in distilled water owing to its porous morphology and the presence of various polar groups. When exposed to a dry environment, it released the absorbed moisture slowly over a period of more than 2 weeks. The urea release rate was also relatively slow; it took about 30 days to release the urea loaded in UCNF. The results indicated that urea diffusion from the material was non-Fickian in character and fit well with the pseudo-second-order kinetics model. The hydrogel demonstrated excellent biodegradability; more than 80% by weight of the hydrogel disappeared through biodegradation by soil bacteria within 3 months. The plant study revealed that adding UCNF to the soil effectively enhanced the seed germination and growth of the plant. Also, plants treated with urea-loaded hydrogel had better growth than the pure urea- and control plant samples. The study found that crosslinked and functionalized cellulose nanofiber may effectively transport water and fertilizer slowly and sustainably.

A Review on the Comparative Analysis of Synthetic Insect Repellents and Essential Oil Based Sustained-release Insect Repellent Formulations

The necessity of developing insect repellents with highly effective sustained release properties has attracted more attention due to the vast spread of arthropod-borne diseases such as dengue and malaria, which must be controlled with proper measures, and also due to the devastating damage caused by severe infestation of insect pests on crops and stored food. Natural and synthetic insect repellents are used against insect vectors and insect pests. Topical insect repellent formulations should be less toxic, less irritant, and less skin permeable. Irrespective of the type and form of the repellent, the mechanism of action depends on the formation of a vapour barrier/ odour barrier, that would deviate the insect from reaching the host. Synthetic insect repellents are highly efficient but due to their high toxicity, degradation resistance, and bio accumulation, they raise environmental and ecological issues. Due to the adverse effects caused by synthetic insect repellents, more attention is given to insect repellents consisting of natural products.Essential oils are among the widely used natural insect repellents available due to their biocompatibility and non-toxicity. High volatility of essential oils is the major problem that hampers the application of essential oils as insect repellent agents. The high volatility that reduces the length of application, and its activity can be successfully addressed by the formulation of slow-release insect repellent composites by incorporating essential oils into adsorbing or encapsulating matrices. Zeolites, montmorillonite, β-cyclodextrin, polymeric materials, and electrospun nanomaterials are commonly used to develop slow-release formulations with essential oils. Among these, polymeric microcapsules are the most extensively studied and developed slow-release insect repellent systems that are based on natural and synthetic active compounds.&#x0D; Key words: Formulation, Insects, Microcapsules, Repellents, Zeolite

Efficient Delivery of Antimicrobial Peptides in an Innovative, Slow-Release Pharmacological Formulation.

Both nanostructure and multivalency enhance the biological activities of antimicrobial peptides (AMPs), whose mechanism of action is cooperative. In addition, the efficacy of a particular AMP should benefit from a steady concentration at the local place of action and, therefore, from a slow release after a dynamic repository. In the context of emerging multi-resistant bacterial infections and the urgent need for novel and effective antimicrobial drugs, we tested these concepts through the engineering of four AMPs into supramolecular complexes as pharmacological entities. For that purpose, GWH1, T22, Pt5, and PaD, produced as GFP or human nidogen-based His-tagged fusion proteins, were engineered as self-assembling oligomeric nanoparticles ranging from 10 to 70 nm and further packaged into nanoparticle-leaking submicron granules. Since these materials slowly release functional nanoparticles during their time-sustained unpacking, they are suitable for use as drug depots in vivo. In this context, a particular AMP version (GWH1-NIDO-H6) was selected for in vivo validation in a zebrafish model of a complex bacterial infection. The GWH1-NIDO-H6-secreting protein granules are protective in zebrafish against infection by the multi-resistant bacterium Stenotrophomonas maltophilia, proving the potential of innovative formulations based on nanostructured and slowly released recombinant AMPs in the fight against bacterial infections.

Fungicidal activity of slow-release formulations of tebuconazole and epoxiconazole to control root rot pathogens of cereal crops

This study attempts to develop a new way to deliver fungicides that will reduce the concentration of chemicals in the soil, while maintaining the effectiveness, provide a prolonged effect of the fungicide and reduce the number of treatments during the growing season. For this purpose, the systemic fungicides tebuconazole and epoxiconazole were embedded in biodegradable matrix consisting of homopolymer poly(3-hydroxybutyrate) and wood flour. Dry components were mixed and formed as granules, which were introduced into the soil with cereal seeds - spring wheat (Triticum aestivum L.) and spring barley (Hordeum vulgare L.). The control groups included intact plants and plants treated with free forms of fungicides. The response of plants to the use of slow-release and free forms of fungicides was assessed by the morphometry of the aerial part and production of carbonylated proteins, malondialdehyde, and proline in plant root cells. The fungicidal activity of the preparations was evaluated by the effectiveness of plant pathogenic fungi suppressing in the soil and on plant roots during the growing season. The results showed that the effect of embedded and free fungicides on redox homeostasis in wheat and barley roots varied depending on the plant growth stage and differed significantly between plant species. The embedded fungicides were more effective in reducing root infection than the free analogues. The use of fungicides in granules led to a decrease in the incidence of root rot in both cereals by 1.5–1.9 times and an increase in the dry mass of plants by 20–28 % in wheat and by 15–18 % in barley. Fungicidal preparations embedded in a degradable base provide long-term protection of plants from soil pathogens and the root diseases control.

Field Evaluation of Slow-Release Wax Formulations: A Novel Approach for Managing Bactrocera zonata (Saunders) (Diptera: Tephritidae)

Chemical management of the peach fly, Bactrocera zonata has been compromised due to adverse effects of pesticide residues that not only contaminate environment but also affect non-target organisms including beneficial insects, birds, aquatic life, and soil microorganisms. They can be impacted through direct exposure or by consuming contaminated prey or plants. The present study was designed keeping in view this increasing demand of the consumers to get pesticide residue free fruit and vegetable produce because it reflects the growing consumer concern for food safety and environmental sustainability, motivating the need for alternative pest management strategies. The field experiment was conducted to determine the best slow-release formulation prepared by mixing the following five different types of waxes, including Candelilla wax (CanW), Paraffin wax (PW), Carnauba wax (CarW), Lanolin wax (LW) and Bees wax (BW) with methyl eugenol (ME) (to attract male B. zonata). The selection of the five different types of waxes was likely based on their biodegradability, availability, and potential for slow-release properties. The result revealed that formulations containing SRF-7[LW], SRF-9[CanW], SRF-8[BW], SRF-9[CarW] and SRF-9[PW] exhibited the maximum capture of 42.10 ± 8.14, 43.30 ± 1.76, 34.30 ± 2.96, 35.30 ± 3.18 and 22.70 ± 3.18 male B. zonata per trap per day, respectively. These effective formulations were further evaluated in experiment in which the comparative trapping efficiency of each wax formulation was assessed. The results demonstrated that formulation containing SRF-9[CanW] was expressed maximum capture 13.77 ± 1.26 male B. zonata per trap per day. These formulations were further evaluated in another experiment in which the trapping efficiency was assessed by four different application methods (simple bottle trap, simple bottle trap with water, yellow sticky trap and jute piece with sticky material). The results demonstrated that formulation containing SRF-9[CarW] applied by yellow sticky trap (YST) trapped 61.74 + 7.69 male B. zonata per trap per day and proved more effective. This formulation can be recommended for trapping and management of male population of B. zonata in fruit orchards. This study can influence eco-friendly B. zonata pest control policies, reducing chemical pesticide usage and promoting agricultural sustainability. Future research should study the long-term impact of slow-release formulations on agricultural sustainability, including pest control, crop yield, and agroecosystem health.

Assessing phytotoxicity and cyto-genotoxicity of two insecticides using a battery of in-vitro biological assays.

Intensive use of chemical pesticides in agriculture poses environmental risks and may have negative impacts on agricultural productivity. The potential phytotoxicity of two chemical pesticides, chlorpyrifos (CPS) and fensulfothion (FSN), were evaluated using Cicer arietinum and Allium cepa as model crops. Different concentrations (0-100 μgmL-1) of both CPS and FSN decreased germination and biological attributes of C. arietinum. High pesticide doses significantly (p≤0.05) caused membrane damage by producing thiobarbituric acid reactive substances (TBARS) and increasing proline (Pro) content. Pesticides elevated ROS levels and substantially increased the superoxide anions and H2O2 concentrations, thus aggravating cell injury. Plants exposed to high pesticide dosages displayed significantly higher antioxidant levels to combat pesticide-induced oxidative stress. Ascorbate peroxidase (APX), guaiacol peroxidase (GPX), catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) increased by 48%, 93%, 71%, 52% and 94%, respectively, in C. arietinum roots exposed to 100 µgFSNmL-1. Under CLSM, pesticide-exposed C. arietinum and 2',7'-dichlorodihydrofluorescein diacetate (2'7'-DCF) and 3,3'-diaminobenzidine stained roots exhibited increased ROS production in a concentration-dependent manner. Additionally, enhanced Rhodamine 123 (Rhd 123) and Evan's blue fluorescence in roots, as well as changes in mitochondrial membrane potential (ΔΨm) and cellular apoptosis, were both associated with high pesticide dose. Allium cepa chromosomal aberration (CAs) assay showed a clear reduction in mitotic index (MI) and numerous chromosomal anomalies in root meristematic cells. Additionally, a-dose-dependent increase in DNA damage in root meristematic cells of A. cepa and conversion of the super-coiled form of DNA to open circular in pBR322 plasmid revealed the genotoxic potential of pesticides. The application of CPS and FSN suggests phytotoxic and cyto-genotoxic effects that emphasize the importance of careful monitoring of current pesticide level in soil before application and addition at optimal levels to soil-plant system. It is appropriate to prepare both target-specific and slow-release agrochemical formulations for crop protection with concurrent safeguarding of agroecosystems.

Emerging Therapies in Hypothyroidism.

Levothyroxine (LT4) is effective for most patients with hypothyroidism. However, a minority of the patients remain symptomatic despite the normalization of serum thyrotropin levels. Randomized clinical trials including all types of patients with hypothyroidism revealed that combination levothyroxine and liothyronine (LT4+LT3) therapy is safe and is the preferred choice of patients versus LT4 alone. Many patients who do not fully benefit from LT4 experience improved quality of life and cognition after switching to LT4+LT3. For these patients, new slow-release LT3 formulations that provide stable serum T3 levels are being tested. In addition, progress in regenerative technology has led to the development of human thyroid organoids that restore euthyroidism after being transplanted into hypothyroid mice. Finally, there is a new understanding that, under certain conditions, T3 signaling may be compromised in a tissue-specific fashion while systemic thyroid function is preserved. This is seen, for example, in patients with metabolic (dysfunction)-associated fatty liver disease, for whom liver-selective T3-like molecules have been utilized successfully in clinical trials.