Background: Sleep quality is commonly defined in terms of total sleep time, onset latency, sleep efficiency and others. Sleep disturbances are a common health complaint that is expected to surge and have more important consequences in adults, especially in health science students, namely nursing students and midwifery students. Moreover, we have recorded a few studies that have been carried out on sleep quality. Objectives: This study aims to survey the current level of sleep quality among nursing students at Can Tho University of Medicine and Pharmacy and determine factors affecting students' sleep quality. Materials and methods: A descriptive cross-sectional research was conducted on 294 nursing students at the Faculty of Nursing - Medical Technology, Can Tho University of Medicine and Pharmacy from June to August 2023 through a self-report questionnaire. The participants were 294 nursing students, including nursing and midwifery students. The selfreport questionnaire included the Pittsburgh Sleep Quality Index (PSQI) and some questions about the characteristics of research subjects such as the features of external environments, learning activities, and personal activities. The PSQI in the study was used in the Vietnamese, and the reliability was evaluated by Trinh My Linh and colleagues with Cronbach's alpha reliability of 0.67. The data were analyzed using the chi-square test and logistic regression. Results: Our study involved 189 students (64.29% response rate). The total students' sleep quality score was 7.18 ± 3.63, which indicated 71.43% of students had sleep disorders. The major factors were self-study time, being disturbed by light in the bedroom and using mobile devices before bed, which were factors related to students' sleep quality (p<0.05). Conclusion: The portion of sleep disorders among nursing students reached large figures. Henceforth, training institutions need to develop health education programs about enhancing the quality of sleep as well as personal lifestyles and self-study methods to help orient and improve the quality of training and quality of life of students.