Skeletal Components Research Articles

Downregulation of Genes for Skeletal Muscle Extracellular Matrix Components by Cisplatin.

The extracellular matrix (ECM) in skeletal muscle is involved in a variety of physiological functions beyond the mechanical support of muscle tissue, nerves, and blood vessels; however, the role of the ECM in skeletal muscle remains unclear. There is little information regarding changes in the expression of factors comprising the ECM during cisplatin-induced muscle atrophy. In the present study, we examined the changes in gene expressions for skeletal muscle extracellular matrix components in skeletal muscle during cisplatin-induced muscle atrophy. Intraperitoneal administration of cisplatin caused muscle atrophy in mice and during this cisplatin-induced muscle atrophy, the expression of many procollagen genes (Col1a1, Col1a2, Col3a1, Col4a1, Col5a1, and Col5a2), elastin (Eln), fibronectin (Fn1), Laminin (Lama1, Lama2, and Lamb1) decorin (Dcn), heparan sulphate proteoglycans (Hspg2) and integrin (Itgb1) constituting the ECM was suppressed. Additional studies are needed to elucidate the pathological significance and mechanisms of reduced gene expression of ECM components associated with cisplatin-induced muscle atrophy.

The MyoGravity project to study real microgravity effects on human muscle precursor cells and tissue

Microgravity (µG) experienced during space flights promotes adaptation in several astronauts’ organs and tissues, with skeletal muscles being the most affected. In response to reduced gravitational loading, muscles (especially, lower limb and antigravity muscles) undergo progressive mass loss and alteration in metabolism, myofiber size, and composition. Skeletal muscle precursor cells (MPCs), also known as satellite cells, are responsible for the growth and maintenance of muscle mass in adult life as well as for muscle regeneration following damage and may have a major role in µG-induced muscle wasting. Despite the great relevance for astronaut health, very few data are available about the effects of real µG on human muscles. Based on the MyoGravity project, this study aimed to analyze: (i) the cellular and transcriptional alterations induced by real µG in human MPCs (huMPCs) and (ii) the response of human skeletal muscle to normal gravitational loading after prolonged exposure to µG. We evaluated the transcriptomic changes induced by µG on board the International Space Station (ISS) in differentiating huMPCs isolated from Vastus lateralis muscle biopsies of a pre-flight astronaut and an age- and sex-matched volunteer, in comparison with the same cells cultured on the ground in standard gravity (1×g) conditions. We found that huMPCs differentiated under real µG conditions showed: (i) upregulation of genes related to cell adhesion, plasma membrane components, and ion transport; (ii) strong downregulation of genes related to the muscle contraction machinery and sarcomere organization; and (iii) downregulation of muscle-specific microRNAs (myomiRs). Moreover, we had the unique opportunity to analyze huMPCs and skeletal muscle tissue of the same astronaut before and 30 h after a long-duration space flight on board the ISS. Prolonged exposure to real µG strongly affected the biology and functionality of the astronaut’s satellite cells, which showed a dramatic reduction of responsiveness to activating stimuli and proliferation rate, morphological changes, and almost inability to fuse into myotubes. RNA-Seq analysis of post- vs. pre-flight muscle tissue showed that genes involved in muscle structure and remodeling are promptly activated after landing following a long-duration space mission. Conversely, genes involved in the myelination process or synapse and neuromuscular junction organization appeared downregulated. Although we have investigated only one astronaut, these results point to a prompt readaptation of the skeletal muscle mechanical components to the normal gravitational loading, but the inability to rapidly recover the physiological muscle myelination/innervation pattern after landing from a long-duration space flight. Together with the persistent functional deficit observed in the astronaut’s satellite cells after prolonged exposure to real µG, these results lead us to hypothesize that a condition of inefficient regeneration is likely to occur in the muscles of post-flight astronauts following damage.

Distraction of longitudinally split fragments using the Ilizarov method: a series of clinical cases of treating partial bone defects

Background. The Ilizarov method is a recognized technique for treating severe skeletal injuries, allowing for a comprehensive restoration of both bone and soft tissue components. Despite the fact that bone lengthening and transport are widely known techniques, distraction of a longitudinally split fragment is still used extremely rarely. The aim of the study is to describe a series of clinical cases involving patients operated on using this technique. Methods. We present a series of observations of five patients who underwent distraction of a longitudinally split fragment using the Ilizarov method between January 2006 and December 2022. Clinical information was obtained from case histories, all surgical interventions were documented. Postoperative examination was performed using radiography. Results. A case series demonstrates the successful application of this technique for reconstruction of partial bone defects resulting from trauma or osteomyelitis. The study included five patients (4 men and 1 woman) who underwent surgery 4.8-34.0 months after trauma for a partial defect of the proximal tibia ranging from 4 to 8 cm in length. Distraction was performed in different directions along the sagittal and longitudinal axes. The time of external fixation ranged from 3.5 to 4.8 months, the external fixation index ranged from 0.49 to 1.22. The ASAMI (Association for the Study and Application of the Methods of Ilizarov) functional score at the follow-up examination was excellent in all five patients. The ASAMI bone tissue assessment showed excellent results in all patients, except for one patient with residual equinus (good result). No other complications were reported. Conclusions. The Ilizarov method provides a minimally invasive and comprehensive approach to the elimination of partial bone defects, affecting simultaneously the skeletal and soft tissue components. Due to the longitudinal splitting during fragment transport and distraction osteogenesis, this method promotes bone and tissue regeneration and helps to avoid a volumetric bone defect and more complex segmental bone transport. Moreover, the role of transverse transport of the tibial cortex increases in the treatment of peripheral arterial diseases.

Plastic deformation behavior and energy absorption performance of a composite metamaterial based on asymmetric auxetic lattices

This study focuses on the design, behavior and experimental analysis of a novel metamaterial, consisting of an asymmetric auxetic three-dimensional structure (AATS) infused with polyester resin. Utilizing FDM additive printing, samples were created with customizable responses to compressive loads through varied design parameters. The objective is to surpass traditional material blending by enhancing stiffness and energy absorption. Striking a delicate balance, the AATS energy absorption properties are preserved while leveraging the stiffness of the resin. Despite its compact cubic form, not exceeding 27 mm on each side, this metamaterial showcases amplified characteristics, blending the AATS and polyester resin. The results hint at promising applications across military defense, automotive, aerospace sectors, and even potential replacements for articulated human skeletal components.

Anatomical Variation of Sternal Angle and Body: a Cross sectional study

Background: The sternum is one of the important skeletal components ofthoracic case that frequently has congenital defects, and researchers routinely use variances to ascertain an individual's age and sex. Additionally, it helps the physician with biopsy procedures as well as therapy. The objective of this study was to evaluate the morphological characteristics of sternal body and sternal angle in both sexes. Method: This cross-sectional stuywas carried out at the Department of Anatomy, Sylhet MAG Osmani Medical College. For this purpose, 100 dried ossified sternums of unknown sex had been collected. The sample was collected in compliance with the inclusion and exclusion criteria. Discriminant function analysis was used to determine the sex of each sternum (DFA). Study variables were the maximum length of sternal body, maximum breadth of sternal body, thickness of the sternal body and sternal angle. Variables were measured using a vernier caliper and documented in a pre-designed data sheet. Result: The mean length of the body of sternum was 93.84±23.31 mm in male and 82.84±14.8 mm in female. The mean width of the body of the sternum was 35.3±10.83 mm in male and 29.22±9.65 mm in female. The thickness of the body of the sternum is 19.86±9.91 mm in males and 16.12±7.39 mm in females. Analysis revealed that length of body sternum, width of body of sternum and thickness of body of sternum were higher in male than female which was statistically significant (p<0.05).The mean angle of sternum was 148.29±8.240 degrees in males and 149.32±9.690degrees in females, respectively. The difference was statistically not significant (p<0.05). Conclusion: In females, the maximum sternal length, maximum sternal breadth, and maximum sternal thickness were all significantly lower.

Object detection algorithms to identify skeletal components in carbonate cores

Identification of constituent grains in carbonate rocks requires specialist experience. A carbonate sedimentologist must be able to distinguish between skeletal grains that change through geological ages, preserved in differing alteration stages, and cut in random orientations across core sections. Recent studies have demonstrated the effectiveness of machine learning in classifying lithofacies from thin section, core, and seismic images, with faster analysis times and reduction of natural biases. In this study, we explore the application and limitations of convolutional neural network (CNN) based object detection frameworks to identify and quantify multiple types of carbonate grains within close-up core images of carbonate lithologies. We compiled nearly 400 images of high-resolution core images from three ODP and IODP expeditions. Over 9000 individual carbonate components of 11 different classes were manually labelled from this dataset. Using pre-trained weights, a transfer learning approach was applied to evaluate one-stage (YOLO v5) and two-stage (Faster R–CNN) detectors under different feature extractors (CSP-Darknet53 and ResNet50-FPN, respectively). Despite the current popularity of one-stage detectors, our results show Faster R–CNN with ResNet50-FPN backbone provides the most robust performance, achieving 0.73 mean average precision (mAP). Furthermore, we extend the approach by deploying the trained model to two ODP sites from Leg 194 that were not part of the training set (ODP Sites 1196 and 1199), providing a performance comparison with benchmark human interpretation.

Evaluating stress and displacement in the craniomandibular complex using Twin Block appliances at varied angles: A finite element study

ObjectivesThe objective of this investigation was to assess the stress and displacement pattern of the craniomandibular complex by employing finite element methodology to simulate diverse angulations of inclined planes that are incorporated in the Twin Block appliance. MethodsA 3D finite element representation was established by use of Cone Beam Computed Tomography (CBCT) scans. This comprehensive structure included craniofacial skeletal components, the articular disc, a posterior disc elastic layer, dental elements, periodontal ligaments, and a Twin Block appliance. This investigation is the first to incorporated inclined planes featuring three distinct angulations (45, 60, and 70°) as the study models. Mechanical impacts were evaluated within the glenoid fossa, tooth, condylar, and articular disc regions. ResultsIn all simulations, the stress generated by the Twin Block appliance was distributed across teeth and periodontal ligament, facilitating the anterior movement of mandibular teeth and the posterior displacement of maxillary teeth. Within the temporomandibular joint region, compressive forces on the superior and posterior facets of the condyle diminished, coinciding with the stress configuration that fosters condylar and mandibular growth. Stress dispersion homogenized in the condylar anterior facet and articular disc, with considerable tensile stress in the glenoid fossa's posterior aspect conforming to stress distribution that promote fossa reconfiguration. The 70° inclined plane exerts the highest force on the tissues. The condyle's maximum and minimum principal stresses are 0.36 MPa and −0.15 MPa, respectively, while those of the glenoid fossa are 0.54 MPa and −0.23 MPa. ConclusionThree angled appliances serve the purpose of advancing the mandible. A 45° inclined plane relative to the occlusal plane exerts balanced anteroposterior and vertical forces on the mandibular arch. Steeper angles yield greater horizontal forces, which may enhance forward growth and efficient repositioning.

Exploring the Significance of Katikataruna Marma: An Ayurvedic Perspective on Vital Energy Center

This review article delves into the intricate concept of Marma points within the framework of Ayurvedic philosophy, with a specific focus on the Katikataruna Marma located in the Prushta region. Marma points, regarded as vital energy centers, house Prana, and hold profound implications for health. The Katikataruna Marma, characterized as a Kalantarapranahara Marma, embodies a delicate balance of Soumya and Agneya qualities, with their interplay dictating the trajectory of life. The precise location of Katikataruna Marma is explored across references, positioned either beside the vertebral column or above the Sphik on the Shronikarna. Anatomically, this Marma amalgamates diverse components: muscular elements such as the gluteal and pelvic muscles, vascular intricacies involving the internal iliac artery, neural and connective structures like sacro-iliac ligaments and lumbo-sacral nerves, skeletal components comprising the iliac bone and sacrum, and the pivotal sacro-iliac joint. Critical insights emerge regarding the aftermath of injuring the Katikataruna Marma. A disruption in the equilibrium of Soumya and Agneya Guna triggers a gradual decline, with blood loss (Shonita kshaya) inducing symptoms like anemia, pallor, malaise, and ultimately, mortality. Prognosis unfolds, emphasizing the manifestation of Pandu, Vaivarna, and Hina Rupa, elucidating the complex interplay between components in the Marma region. By dissecting the intricacies of the Katikataruna Marma, this article contributes to a deeper comprehension of Ayurvedic principles and their holistic approach to wellness. The exploration of Marma points transcends anatomical facets, resonating with Ayurveda's holistic philosophy of harmonizing physical, mental, and spiritual well-being.

Artificial intelligence as a prediction tool for orthognathic surgery assessment.

An ideal orthodontic treatment involves qualitative and quantitative measurements of dental and skeletal components to evaluate patients' discrepancies, such as facial, occlusal, and functional characteristics. Deciding between orthodontics and orthognathic surgery remains challenging, especially in borderline patients. Advances in technology are aiding clinical decisions in orthodontics. The increasing availability of data and the era of big data enable the use of artificial intelligence to guide clinicians' diagnoses. This study aims to test the capacity of different machine learning (ML) models to predict whether orthognathic surgery or orthodontics treatment is required, using soft and hard tissue cephalometric values. A total of 920 lateral radiographs from patients previously treated with either conventional orthodontics or in combination with orthognathic surgery were used, comprising n = 558 Class II and n = 362 Class III patients, respectively. Thirty-two measures were obtained from each cephalogram at the initial appointment. The subjects were randomly divided into training (n = 552), validation (n = 183), and test (n = 185) datasets, both as an entire sample and divided into Class II and Class III sub-groups. The extracted data were evaluated using 10 machine learning models and by a four-expert panel consisting of orthodontists (n = 2) and surgeons (n = 2). The combined prediction of 10 models showed top-ranked performance in the testing dataset for accuracy, F1-score, and AUC (entire sample: 0.707, 0.706, 0.791; Class II: 0.759, 0.758, 0.824; Class III: 0.822, 0.807, 0.89). The proposed combined 10 ML approach model accurately predicted the need for orthognathic surgery, showing better performance in Class III patients.

Evaluating the Effects of Carriere Motion Appliance and Twin Block Appliances in Class II Correction-A Retrospective Study.

The treatment of 38 patients was assessed. Pre- and post-treatment cephalometric radiographs were analyzed to evaluate skeletal, dental, and soft tissue treatment outcomes and efficacy. Both appliances effectively corrected the Class II molar relationship. When measured at the distal aspect of the first molar, TB achieved 4.22 mm, while CMA had a 2.55 mm correction. When measured in the mesial aspect, the CMA achieved a 3.9 mm correction. The changes in SNB and ANB were statistically significant only in the TB group. The CMA appliance demonstrated statistically significantly less protrusion of the mandibular incisors and less upper incisor retrusion without vertical changes compared to the TB appliance. The TB demonstrated statistically significant lower lip protrusion compared to the CMA. The CMA corrects Class II malocclusions only by exerting a dentoalveolar influence and does not demonstrate the added effects associated with TB, such as elongation of lower facial height (LFH) and less loss of lower anchorage. Nonetheless, the correction in the TB group comprised both dentoalveolar and skeletal components. The CMA promotes a multidirectional upper and lower molar movement, and despite our 2D cephalometric analysis, we were able to estimate the extent of upper molar derotation.

Using shell to enhance the phosphorus adsorption capability of aluminum-based drinking water treatment residue-dominant sintered ceramsite

Al-based drinking water treatment residue (DWTR) is an inevitable byproduct of potable production that can provide skeletal components to make sintered ceramsite for water treatment. However, sintering promotes Al aging and decreases its adsorption capability. This study proposes and verifies a strategy for using shell, a common natural Ca-based material, to enhance P adsorption by Al-based DWTR-dominant ceramsite. The results showed that the shell mainly acted as an expanding component, and thermolysis of CaCO3 to form CaO was the main expanding mechanism, further enhancing the coupling effect of Fe, K, and Si for sintering. This mechanism led to a reduction in the cylinder compressive strength with increasing shell proportions and an increase in the broken rate and cylinder compressive strength with increasing temperature. However, all DWTR shell-based ceramsites are non-hazardous. Importantly, the shell increased the P adsorption capacity of the ceramsite by approximately two orders of magnitude and caused the adsorbed P to remain as HCl-extractable and residual P. A logistic equation (r2 = 0.923, n = 10) was established for the cylinder compressive strength to Si, K, Fe, and Ca in ceramsite and loss on ignition in raw materials to provide a potential reference for ceramsite production.

Controllable and reusable seesaw circuit based on nicking endonucleases

Seesaw circuits are essential for molecular computing and biosensing. However, a notable limitation of seesaw circuits lies in the irreversible depletion of components, precluding the attainment of system recovery and rendering nucleic acid circuits non-reusable. We developed a brand-new method for creating controllable and reusable seesaw circuits. By using the nicking endonucleases Nt.BbvCI and Nt.Alwi, we removed “functional components” while keeping the “skeletal components” for recurrent usage. T-inputs were introduced, increasing the signal-to-noise ratio of AND logic from 2.68 to 11.33 and demonstrating compatibility. We identified the logic switching feature and verified that it does not impair circuit performance. We also built intricate logic circuits, such as OR-AND gate, to demonstrate the versatility of our methodology. This controllable reusability extends the applications of nanotechnology and bioengineering, enhancing the practicality and efficiency of these circuits across various domains.

Assessment of pharyngeal airway space in patients with different maxillofacial skeletal abnormalities using cone beam computed tomography

Background. The patency of the pharyngeal airway has a far-reaching impact on craniofacial development. Conversely, the position of the maxillofacial skeletal components in certain disorders may lead to a compromised airway. Therefore, the evaluation of the airway is an essential step in treatment planning. Objectives. The basic aim of this study was to measure the pharyngeal air space volume in the patients with maxillofacial skeletal abnormalities (like Class II, Class III malocclusion, TMJ Ankylosis, Condylar abnormalities and Syndromic cases) and those without any skeletal abnormalities. The objectives were to assess the Linear, Cross-sectional, and Volumetric dimensions of the pharyngeal airway in patients with and without maxillofacial skeletal abnormalities. (Study and Control group) Materials and methods. The Study group included 49 patients with maxillofacial Skeletal abnormalities like Class II and Class III malocclusion, Temporomandibular Joint Ankylosis, Condylar abnormalities, Syndromic cases. 49 Control group patients did not present with any skeletal abnormalities. The Linear, Cross-sectional, and Volumetric dimensions of the pharyngeal airway in the Study group and Control group were calculated and compared within the groups. Statistical Analysis: Unpaired t -test, ANOVA test and Tukey’s Post Hoc analysis test were for the data analysis of the above study. Outcomes. The Anteroposterior dimension of the airway at the Superiormost level was found to be significantly greater in Class II (p-value-0.039), Class III (p-value-0.002), and Control groups (p-value <0.001) when compared with the TMJ Ankylosis group. The Volume and Cross-sectional area of the airway at the most constricted level of the airway was found to be significantly greater in Class III (p-value <0.001) and Control group (p-value 0.013, 0.003) respectively when compared with the TMJ Ankylosis group. Conclusions. The pharyngeal airway was narrowest anteroposteriorly at all three levels (Superior-most, Most constricted, and Inferior-most levels) in the TMJ Ankylosis group and widest in the Class III group. The least cross-sectional area was found in the TMJ Ankylosis group whereas the greatest was found in the Class III group. The airway volume in the TMJ Ankylosis group and Class II group is significantly lesser than that of the Control group.

Abstract 3405: CT-assessed body composition and tumor immunologic characteristics in patients with non-small-cell lung cancer

Abstract Objectives: Obesity is associated with higher immunotherapy efficacy, but body mass index is a convoluted marker of adipose and muscle tissue. We conducted a discovery scale study to assess the association of skeletal muscle and adipose tissue areas annotated on computed tomography (CT) scan images with selected tumor immunologic characteristics among patients with non-small-cell lung cancer (NSCLC). Methods: We analyzed publicly available data from 102 patients (mean age = 67.2, 47.1% female, and 70.6% stage I-II) who had diagnostic CT images in The Cancer Image Archive. All patients had the 12th thoracic vertebra (T12) image, and a subset (62 patients) had the 3rd lumber vertebra (L3) image. Paravertebral muscle areas on T12 and skeletal muscle and adipose tissue areas on L3 were annotated using Slice-O-Matic. RNA-seq data were provided by The Cancer Genome Atlas and Clinical Proteomic Tumor Analysis Consortium; percentages of CD8, regulatory T cells, and activated NK cells were identified using the CIBERSORT. ESTIMATE algorithm was used to derive the stromal score and the immune score. Differences in the immune markers were examined using ANOVA adjusting for stage, histology, and study. Results and Conclusion: Patients with higher paravertebral muscle area (3rd tertile) had the lowest stromal scores, compared to the 2nd and 1st tertile (P<0.05). Patients in the 2nd tertile of paravertebral muscle areas had the highest immune score and CD274 (PD-L1) gene expression. Among the body composition types classified using L3 images, low muscle/high adiposity was associated with lower stromal scores, and low muscle was associated with lower immune scores, compared to high adiposity and high muscle/low adiposity types. Other immunological markers did not differ across the body composition types. Significance: These findings suggest associations between deconvoluted skeletal and adipose tissue components and tumor markers relevant to immune checkpoint inhibitor efficacies in NSCLC. Further validation using immune phenotyping in a large patient sample is warranted. Funding: The study is in part supported by NIH/NCI R37CA248371 and OSU Comprehensive Cancer Center - James. Citation Format: Ting-Yuan David Cheng, Cuthbert Mario Mahenge, Rand T. Akasheh, Ayse Selen Yilmaz, Ben Kinder, Xuan Nguyen, Maciej Pietrzak, Carolyn J. Presley, Peter G. Shields. CT-assessed body composition and tumor immunologic characteristics in patients with non-small-cell lung cancer [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 3405.

Exploring Age and Gender Identification Through Mandibular Parameters Using Orthopantomography: An Observational Study.

Aim This study aimsto examine five mandibular parameters: coronoid ramus height, condylar ramus height, projective ramus height, minimum ramus breadth, and gonial angle, using orthopantomography (OPG). Introduction The mandible, a crucial part of the human skull, demonstrates sexual dimorphism, which makes it an important tool for determining sex in forensic and anthropological investigations.Its form and structure are relatively resistant to significant changes after death. Among all skeletal components, the mandible stands out as a primary indicator of sexual differences. In forensic investigations, establishing the age and sex of an individual is considered a crucial initial step. This process can be particularly challenging in scenarios involving mass casualties, natural calamities, or extensively fragmented remains. Due to its responsiveness to growth patterns, the mandible is adept at accurately determining both age and sex. Materials and methods This study employed a sample size of 500 individuals, split equally between males and females, with 250 participants each. The age bracket chosen for this cohort ranged from 20 to 30 years, considering that bone growth characteristics within this range can assist in sex determination. The height of the mandibular ramus was assessed using Planmeca software, and subsequent data analysis was conducted using SPSS. Results When estimating age, the condylar ramus height exhibited the smallest standard error (0.010), whereas the maximum standard error for the gonial angle was 0.028. Positive t values were observed for the gonial angle (1.182), minimum ramus breadth (0.114), and coronoid ramus height (0.733). In terms of determining sex, the gonial angle, coronoid ramus height, and projective ramus height demonstrated positive coefficient functions, specifically 0.676, 0.090, and 0.286, respectively. Conversely, both the minimum ramus breadth and the condylar ramus height displayed negative values of -0.385 and -0.126, respectively. Conclusion Among the parameters evaluated, condylar ramus height emerges as the most suitable choice for estimating age, while gonial angle, coronoid ramus height, and projective ramus height are preferable for determining sex.

Testing the effect of functional orthopedic appliance Simões network 6 (SN6) through surface electromyography on rest mandibular position – a pilot study

The maintenance of the lower level of muscle contraction is fundamental for the development and health of the stomatognathic system (SS). Malocclusion leads to unbalance muscle activity, since Class II treatment is not the most prevalent malocclusion in the general population but is the more frequent to search for treatment in dental offices and the most prevalent skeletal component in the class II malocclusion is the retrognathia this pilot investigation aims to study the effect of rest electric activity of bilateral anterior temporal, masseter and suprahyoid muscle in individuals undergoing class II treatment with functional orthopedic appliance Simões Network 6 (SN6). Surface electromyography recordings of bilateral anterior temporal, masseter and suprahyoid muscle in rest position was obtained before and 8 minutes after the functional appliance insertion in mouth. Results showed improvement of electric muscle activity in rest position and balance of bilateral muscle electric activity in all muscle. Based on the preliminary results it can be conclude that jaw functional orthopedics (JFO) provides alteration of the resting records in the mandibular rest position of the studied muscles, indicating relaxation of the muscles studied and a more symmetrical electric activity. Further studies are necessary for a better understanding of the effects of functional orthopedic appliances in the electric activity of stomatognathic muscle in the mandibular rest position.

Upper Jurassic–lowermost Cretaceous hybrid build-ups of the Western Tethys Realm: Cement-rich microencruster-microbialite-calcified sponge framework

This study summarizes and provides new data concerning the composition, fabrics, depositional environment and palaeogeographic distribution of Upper Jurassic–lowermost Cretaceous (Kimmeridgian–Berriasian) upper slope build-ups with complex microencruster-microbialite-calcified sponge frameworks associated with large amount of early marine cement crusts. The focus is on reef carbonates from the Štramberk-type limestones from the Carpathians (Getic Carbonate Platform) and Apuseni Mountains in Romania, with additional data from the Plassen Carbonate Platform (Northern Calcareous Alps, Austria) and the Štramberk Carbonate Platform (Western Carpathians, Czech Republic and Poland). The microencrusters, often of problematic biological affinity, are mainly Crescentiella morronensis (Crescenti 1969), Labes atramentosa Eliášová 1986 and Radiomura cautica Senowbari-Daryan & Schäfer 1979. Specialized encrusting calcified sponges are also common, with Perturbatacrusta leini Schlagintweit & Gawlick 2011 as the most abundant and characteristic form. Light-dependent microencrusters (e.g., “Lithocodium-Bacinella”) that are abundant in coeval overlying coral-dominated reefs, are rare, as are corals (microsolenids adapted to low-light level). Three dominant types of fabric-based framework varieties are considered for the studied build-ups. The main differences between these types reflect a bathymetric zonation in their development. The abundance of Crescentiella, microbialites, massive radiaxial fibrous cement crusts, and poorly diversified microencruster/sponge assemblages, are all suggesting that Type I framework variety is characteristic for build-ups formed in the deepest parts of an upper slope environment. Type II most probably developed at slightly lower depths than Type I, as revealed by its main features (clusters of calcified sponges and microencrusters associated with less extensive cement crusts) and distribution between the other two types in the sedimentary sequences. Type III, the most common, characterizes build-ups formed at the shallowest depths of the upper slope environment and is composed of alternating layers of diverse microencrusters (including several light-dependent species), calcified sponges, rare microsolenid corals, microbialites and early marine cement crusts. The distribution patterns of these framework varieties (Type I to Type III) together with their associated facies, strengthen this depositional interpretation. From a process-based perspective, these upper slope build-ups exemplify triple hybrid carbonates which are intimate combinations of microbial and skeletal components (microencrusters/microbialites and calcified sponges), associated with abiotic precipitates (early marine cement crusts). Their palaeogeographic importance is reflected in their formation on the slopes of rimmed carbonate platform systems facing open oceanic domains, predominantly within the central Western Tethys Realm. Such hybrid build-ups appear to have been absent on the southern European shelf where other reef frameworks commonly developed on carbonate ramps and ramp-type carbonate platforms.

Uncovering the mosaic evolution of the carnivoran skeletal system.

The diversity of vertebrate skeletons is often attributed to adaptations to distinct ecological factors such as diet, locomotion, and sensory environment. Although the adaptive evolution of skull, appendicular skeleton, and vertebral column is well studied in vertebrates, comprehensive investigations of all skeletal components simultaneously are rarely performed. Consequently, we know little of how modes of evolution differ among skeletal components. Here, we tested if ecological and phylogenetic effects led to distinct modes of evolution among the cranial, appendicular and vertebral regions in extant carnivoran skeletons. Using multivariate evolutionary models, we found mosaic evolution in which only the mandible, hindlimb and posterior (i.e. last thoracic and lumbar) vertebrae showed evidence of adaptation towards ecological regimes whereas the remaining skeletal components reflect clade-specific evolutionary shifts. We hypothesize that the decoupled evolution of individual skeletal components may have led to the origination of distinct adaptive zones and morphologies among extant carnivoran families that reflect phylogenetic hierarchies. Overall, our work highlights the importance of examining multiple skeletal components simultaneously in ecomorphological analyses. Ongoing work integrating the fossil and palaeoenvironmental record will further clarify deep-time drivers that govern the carnivoran diversity we see today and reveal the complexity of evolutionary processes in multicomponent systems.

Clinical indications for the diagnosis and treatment of functional posterior crossbite in pediatric population: a narrative review with clinical description.

The present manuscript aims to provide an updated overview of the clinical management of functional posterior crossbite (FPXB) in growing subjects which can be helpful for orthodontists and pediatric dentists in daily practice. Database searches were performed until December 2023 to evaluate the published literature on the topic. The most pertinent articles were chosen for the review from the retrieved articles. No restrictions regarding the year or language of publication were applied. Additional studies were included by manually searching the references of the included studies. The manuscript has been structured for a narrative purpose. Although there is evidence of spontaneous correction in the transition from deciduous to mixed dentition, the literature suggests initiating the treatment early to increase the success rate. Early treatment involves reducing the risk for potential temporomandibular disorders and adaptations at the level of skeletal, dental, and muscle components. Recent advancements in tridimensionali (3D) imaging systems can also help define the appropriate treatment time case-by-case. Considering the prevalence and the multiple etiological factors involved in the development of FPXB in the pediatric population, orthodontists and pediatric dentists should decide the treatment time for this condition with a careful evaluation of the risk/benefit ratio.

Correlation Between Horizontal and Vertical Skeletal Components in Dental Malocclusions Among the Jazan Population.

The dentoskeletal morphology of various malocclusions has been analyzed in cephalometric studies. It is important to understand the vertical and horizontal components of orthodontic treatment. To provide accurate treatment, an orthodontist needs to understand the facial types of an individual. This study aims to evaluate a correlation between vertical and horizontal components of skeletal and dental malocclusion by assessing cephalometric radiographs of the population of Jazan province. The cephalometric radiographs of 267 eligible participants were assessed digitally. Fourteen skeletal and dental parameters were used to evaluate the association. Reliability was checked with the intra-class coefficient. Statistical analyses included descriptive statistics and Spearman's rho test. Statistical significance was set at P ≤ 0.05. Correlations were found between anterior facial height (AFH), posterior facial height (PFH), FH ratio (Jarabak ratio), upper incisor to NA (U1-NA), lower incisor to NB (L1-NB), and upper incisor to the palatal plane (U1/PP). In dental class I, AFH (N-Me) had a strong positive correlation with L1-NB (0.300), U1/PP (0.164), and L1/MP (0.215). In dental class II, AFH negatively correlated with U1-NA (-0.735) and positively correlated with L1-NB (0.292), L1/MP (0.085), and U1-NA. PFH (S-Go) positively correlated with L1-NB (0.525) in class I but negatively correlated in class II. However, a negative relationwas observed between all the vertical and horizontal components in class III. This study suggests potential associations between vertical and horizontal components in developing skeletal and dental discrepancies.