Size Of Stem Cells Research Articles

Effect between Stem Cell Size and In-vitro Culture Microenvironment

Effect between Stem Cell Size and In-vitro Culture Microenvironment

Stem Cell Therapies

Stem cells have the ability to self-replicate in vitro indefinitely under adequate conditions and provided that pluripotent signals are triggered exogenously. Moreover, they are able to differentiate into virtually all human cell types. This vast potential makes stem cells a powerful tool to be used in regenerative medicine and different therapeutic strategies as well as in clinical and basic research, contributing to the generation of novel therapeutic avenues to treat innumerable pathological disorders that today have no known cure. Despite increased disclosures at a high speed pace in terms of benefits, applicability and relevance of stem cell based therapies; more studies in humans are needed to advance in the establishment of safe and effective cell-based treatments. In this review we compile an overview of the latest reports and patents on stem cell accomplishments for the treatment of diseases and injuries from Patent databases as The European Patent Office, WIPO, Fresh patents and Freepatents online, as well as Pubmed references, regarding stem cell therapy. Nevertheless, the vast majority of these promising results have been established in animal models, suggesting that stem cells research must go on prior to transferring therapeutic protocols to the human being although it has much to offer to improve life quality and eliminate disability. Keywords: Cell therapies, engraftment, IPSCs, microvesicles delivered from embryonic stem cells, proliferation stimulating antibodies, stem cell size.

Morphology and size of stem cells from mouse and whale: observational study

Objective To compare the morphology and size of stem cells from two mammals of noticeably different body size.Design Observational study.Setting The Netherlands.Participants A humpback whale (Megaptera novaeangliae) and a laboratory...

Changes in the metabolome and histopathology of Amaranthus hypochondriacus L. in response to Ageratum enation virus infection

Amaranthus hypochondriacus L. infected with Ageratum enation virus (AEV) was investigated for identifying alteration in the anatomical structures, sap translocation and metabolomic variations using light microscopy, magnetic resonance imaging, NMR spectroscopy and GC–MS, respectively. Combination of GC–MS and NMR spectroscopy identified 68 polar and non-polar metabolites that were present in different levels in healthy and virus-infected A. hypochondriacus. Contrast of T1 and T2 weighted MR images showed significant differences in the spatial distribution of water, lipids and macromolecules indicating alterations in the cortical region and disruption of vascular bundles in virus-infected stem tissues. MRI observations are supported by light microscopic examination. Microscopic examination of AEV infected stem revealed severe hyperplasia with a considerable reduction in size of stem cells. The NMR spectroscopy and GC–MS analysis indicated that viral infection significantly affected the plant primary and secondary metabolism resulting in decreased glucose and sucrose content and increase in the concentration of β-sitosterol and stigmasterol. Higher accumulation of TCA cycle intermediates such as citric acid and malic acid in AEV infected plants indicated enhanced rate of respiratory metabolism. The viral stress significantly increases the concentration of erythritol and myo-inositol as compared to healthy ones. Lower concentration of glucose and sucrose in viral-infected stem tissues suggests decreased translocation of photosynthates in the plants. The results demonstrated potential of MRI, NMR spectroscopy and GC–MS for studying anatomical and metabolic variations in virus-infected plants.

Metabolic and histopathological alterations of Jatropha mosaic begomovirus-infected Jatropha curcas L. by HR-MAS NMR spectroscopy and magnetic resonance imaging

Alterations in the anatomical structures, sap translocation and metabolic profiles in Jatropha curcas L. (Euphorbiaceae), infected with Jatropha mosaic virus (JMV) have been investigated using MRI and HR-MAS NMR spectroscopy. The contrast of MRI images distinguishes abnormalities in anatomical structures of infected and healthy stem. The HR-MAS NMR spectroscopic analysis indicated that viral infection significantly affected the plant metabolism. Higher accumulation of TCA cycle intermediates, such as citrate and malate, in JMV-infected plants suggested a higher rate of respiration. The respiration rate was more than twofold as compared to healthy ones. The viral stress also significantly increases the concentrations of alanine, arginine, glutamine, valine, GABA and choline as compared to healthy ones. Microscopic examination revealed severe hyperplasia caused by JMV with a considerable reduction in the size of stem cells. Lower concentration of glucose and sucrose in viral-infected stem tissues indicates decreased translocation of photosynthates from leaves to stem due to hyperplasia caused by JMV. The MR images distinguished stele, cortical and pith regions of JMV-infected and healthy stems. Contrast of T(1)- and T(2)-weighted images showed significant differences in the spatial distribution of water, lipids and macromolecules in virus-infected and healthy stem tissues. The results demonstrated the value of MRI and HR-MAS NMR spectroscopy in studying viral infection and metabolic shift in plants. The present methodology may help in better understanding the metabolic alterations during biotic stress in other plant species of agricultural and commercial importance.