Background: Situs inversus is a rare and silent autosomal recessive disorder occurring in 1:5,000 to 1:20,000 individuals. Laparoscopic cholecystectomy, a standard treatment for gallbladder disease in the general population, is very challenging in patients with situs inversus totalis and acute cholecystitis, especially for right-handed surgeons. We herein report a case involving our modified laparoscopic cholecystectomy technique for right-handed surgeons in a Korean female patient with situs inversus totalis who developed a acute diffuse suppurative calculous cholecystitis. Case presentation: An 83-year-old female patient with dextrocardia presented with a two days history of epigastric and left upper quadrant abdominal pain. Vital signs were stable. White blood cell showed 18,400/uL. C reactive protein was 22.57 mg/dL. Abdominal commputerized tomography revealed a left-sided gallbladder with volumetric increase of the gallbladder and even enhancing thicknesssevere of the gallbladder wall suggesting acute calculous cholecystitis. We performed emergent laparoscopic cholecystectomy with our modified technique including port relocation. The operation went well, and our patient recovered satisfactorily. Conclusion: Laparoscopic cholecystectomy in a patient with a left-sided gallbladder is not often confidently performed by right-handed surgeons. However, some modifications of “mirror image” ports are the keys to successful completion of this operation. The patient will thus still obtain benefits from this standard minimally invasive technique. Keywords: Laparoscopic cholecystectomy, Situs inversus totalis and Acute cholecystitis.