Location Of Sites Research Articles

The Agroecological Transition of Farms in the Ecuadorian Andes Through the Lens of the Main Agroecological Structure

ABSTRACTMany sectors of society have urged a transition from modern conventional agriculture to more sustainable alternatives. One of the most frequently mentioned is agroecology, due to its potential to improve soil health, promote agrobiodiversity, food sovereignty, and regenerate ecosystem functions. However, complex systems such as farms demand the transformation of a broad set of characteristics and practices for its transition. These factors are, in turn, conditioned by social, educational, economic, and political factors. Therefore, a clear understanding of the main drivers behind the management of farms is vital for supporting an effective transition. This research applied the main agroecological structure (MAS) methodology, which employs 10 indicators (5 biological, 5 cultural) to evaluate the degree of connectivity of a farm with the landscape, the agrobiodiversity present in living fences and crops, and the practices employed by farmers to support and preserve it. Finally, the MAS methodology gives farms a score from 0 to 100, understood as the degree of development of its agroecological structure, or as the “resilience” of a system in agroecological transition. Measurements were performed in 20 farms with different degrees of agroecological transition, in five locations of the northern‐central Andean region of Ecuador. The farms had a moderately developed structure on average (71.05 over 100), and MAS variation was not correlated to the size of the farm, nor with its degree of connectivity with the surrounding landscape. It was rather significantly related to the geographic location of the study site. One of the main differences between study sites was a more extended prevalence of cooperation and development programs, suggesting a strong connection between the development of agroecological practices and capacity building processes. For this reason, the author proposes that MAS measurements should be complemented with deeper analyses of the capacity‐building background of each site. This study aims to establish a baseline of the MAS in Ecuador, and to provide new tools for monitoring and supporting agroecological transition.

Combining Remote Sensing Data and Geochemical Properties of Ultramafics to Explore Chromite Ore Deposits in East Oltu Erzurum, Turkey

The present research’s main objective was to apply thorough exploration approaches that combine remote sensing data with geochemical sampling and analysis to predict and identify potential chromitite locations in a complex geological site, particularly in rugged mountainous terrain, and differentiate the ultramafic massif containing chromitite orebodies from other lithologies. The ultramafic massif forming the mantle section of the Kırdağ ophiolite, located within the Erzurum–Kars Ophiolite Zone and emerging in the east of Oltu district (Erzurum, NE Turkey), was selected as the study area. Optimum index factor (OIF), false-color composite (FCC), decorrelation stretch (DS), band rationing (BR), minimum noise fraction (MNF), and principal and independent component analyses (PCA-ICA) were performed to differentiate the lithological features and identify the chromitite host formations. The petrography, mineral chemistry, and whole-rock geochemical properties of the harzburgites, which are the host rocks of chromitites in the research area, were evaluated to verify and confirm the remote sensing results. In addition, detailed petrographic properties of the pyroxenite and chromitite samples are presented. The results support the existence of potential chromitite formations in the mantle section of the Kırdağ ophiolite. Our remote sensing results also demonstrate the successful detection of the spectral anomalies of this ultramafic massif. The mineral and whole-rock geochemical features provide clear evidence of petrological processes, such as partial melting and melt–peridotite interactions during the harzburgite formation. The chromian spinels’ Cr#, Mg#, Fe3+, Al2O3, and TiO2 concentrations indicate that the harzburgite formed in a fore-arc environment. The Al2O3 content and Mg# of the pyroxenes and the whole-rock Al2O3/MgO ratio and V contents of the harzburgite are also compatible with these processes. Consequently, the combined approaches demonstrated clear advantages over conventional chromitite exploration techniques, decreasing the overall costs and supporting the occurrence of chromite production at the site.

Hair follicle-targeted delivery for hair recoloration using scalp-curvature-conforming microneedles based on sodium alginate and polyvinylpyrrolidone

Hair-related disorders are currently widely concerned issues for not only the scientific society but also the public attentions. Microneedle-based drug delivery system has been regarded as a promise hair follicle-targeted drug delivery approach, largely because they can effectively penetrate the stratum corneum barrier and deliver drugs to hair follicles within dermis. However, the currently reported microneedles for treating hair-related disorders usually rely on rigid backings, showing poor adaptability to the curved scalp and thereby restricting their usability for hair follicles targeted drug delivery. To this end, this study utilized sodium alginate and polyvinylpyrrolidone to construct a scalp-curvature-conforming microneedle with flexible backing. Subsequently, Psoralea corylifolia extract (PE) was loaded into the microneedles to investigate its capability in delivering PE to the hair follicle site for treating leukotrichia associated with vitiligo. These PE-loaded microneedles can effectively conform to the curvature of skin, enhancing the efficiency of microneedle insertion and ensuring stable drug delivery. Moreover, animal studies demonstrate that the PE loaded microneedles can effectively penetrate the stratum corneum, benefiting the drug delivery to hair follicles located site, and consequently showing a successful inhibition of hair graying. In summary, the present study reports a design and preparation of scalp-curvature-conforming microneedle. This design may offer a potential solution for efficient drug delivery using microneedles to the curved skin.

Analysis of External Environmental Design Factors on Mosquito Breeding in Sikka Regency, East Nusa Tenggara

Factors such as environmental temperature, rainfall, location of waste disposal sites, sunlight exposure, water flow, as well as chemical, biological, and socio-cultural conditions play a crucial role in the development of malaria cases. This study specifically aims to analyze the influence of environmental elements on the number of malaria and dengue fever (DF) cases in Sikka Regency, as well as to evaluate the physical design elements of the area that can reduce DF cases in the region. The research was conducted in several locations within Sikka Regency, taking into account the differences in location and the distance between buildings in relation to thermal characteristics. The results indicate that areas located more than 500 meters above sea level did not experience malaria or DF cases, which is also influenced by the outdoor environmental design, such as building density and vegetation size, affecting light intensity. Lower light intensity increases humidity, which accelerates mosquito breeding.

Hearth management at a middle Paleolithic rock shelter site: Smoke density analysis at Tor Faraj, Jordan

Paleolithic rock shelters often include several hearths located in different parts of the site. In this paper, we analyze relevant data from Middle Paleolithic Tor Faraj rock shelter as a case study of smoke density in correlation with hearth location and functionality. Since one of the major negative fire products is smoke, which has an immediate effect on human health, we used computer simulations to analyze the effect of various parallel hypothetical hearth configurations on smoke density in the shelter, as categorized by the average smoke exposure recommendations from the World Health Organization (WHO) and Environmental Protection Agency (EPA). The simulations enable us to investigate various hearth activation possibilities affecting smoke density in all the site areas, thus enhancing the understanding of hearth location and functionality in Paleolithic rock shelter sites. The results correspond with ethnographic studies of sites where hearths for sleeping are located at the back wall of the shelter and explain, for the first time, the reason for this placement. The number of parallel active hearths was found to be limited by smoke density factors, despite the ventilation afforded by the rock shelter's high, wide opening, which also left it vulnerable to the effects of wind. The distance between the hearths also influenced smoke density, especially between the drip line up to the rock shelter center. Thus, these areas were probably more suitable for short-duration activities such as cooking. We further concluded that the hearths at Tor Faraj were most probably activated at the time of need and not necessarily simultaneously. By correlating wind statistics from the Meteoblue website with our smoke density simulation results, we could deduce that intensive occupations at Tor Faraj in the middle of winter (January) would have been problematic due to wind affecting smoke dispersal at the site.

Porous Carbon‐Supported Catalysts for Proton Exchange Membrane Fuel Cells

AbstractProton exchange membrane fuel cells (PEMFCs) are crucial for the efficient utilization of hydrogen. Currently, their efficiency is mainly limited by the slow kinetics of the cathode oxygen reduction reaction (ORR) and the poisoning effect between ionomers and catalytic sites, particularly with Pt‐based catalysts. Recent works suggest that the emerging porous carbon‐supported catalysts hold promise in mitigating these challenges by ensuring fast kinetics while alleviating the poisoning. This review examines porous carbon‐supported catalysts for PEMFC cathodes, covering synthesis methods, structure and performance evaluation, and future prospects, with an emphasis on the influence of porous carbon support on PEMFC performance. On one hand, the rational design of pore structure in carbon support can help optimize the location of the active sites and enhance mass transfer. On the other hand, diverse pore structures provide a platform for gaining a deeper understanding of the mechanisms behind microscale mass transfer and reaction at the three‐phase boundaries. This review aims to inspire innovative strategies for the precise synthesis of porous carbon‐supported catalysts with various pore structures to further boost PEMFC performance.

Speckle tracking echocardiography as a predictor of location of ablation sites in candidates to catheter ablation for recurrent ventricular tachycardia

Abstract Background Preoperative imaging is essential before a ventricular tachycardia (VT) ablation procedure, to improve operative outcomes and reduce procedural times. Although MRI is the gold standard, it has several limitations, including patient compliance, possible presence of non-MRI-compatible devices, and long acquisition time. Speckle tracking echocardiography (STE) is a reliable and appealing technique that quantifies regional and global myocardial deformation, as well as layer-specific (endo-, mid- and epicardial) longitudinal strain, without MRI limitations. Purpose This study aimed to evaluate the efficacy of STE in predicting VT ablation sites. Methods In this study, we compared the bull’s eye segmentation of the left ventricle, global and layer-specific, obtained with STE (TomTec Arena), to investigate whether echocardiography can predict the areas of scar and late potential, as identified by electroanatomic mapping (EnSite X mapping, HD Grid mapping catheter, Abbott). Strain analysis and electroanatomic mapping data were matched segment-by-segment to obtain pairs of endocardial strain and bipolar substrate maps, as well as pairs of midmyocardial/epicardial strain and unipolar substrate maps. Results Twenty-three consecutive patients with an indication for catheter ablation for recurrent VT were enrolled. All patients were being treated with beta-blockers, 52% were taking amiodarone, and only 2 patients were on mexiletine. A total of 1170 pairs of bull’s eye segments were available for analysis. A binary logistic mixed-effects model was used to predict the presence of electroanatomical scar based on strain echocardiography data, to account for interpatient variability. The study findings highlighted a close correlation between the bipolar map and endocardial strain values, and between the unipolar map and midmyocardial strain values. Progressively higher (less negative) values of endocardial strain proved predictive of bipolar electroanatomical scar, with an increase in the odds ratio of bipolar electroanatomical scar equal to 6.2% (95% confidence interval 2.5%-10%) for each unit increase in strain values. Similarly, progressively higher values of midmyocardial strain were predictive of unipolar electroanatomical scar, with an increase in the odds ratio of unipolar electroanatomical scar equal to 6.2% (95% confidence interval 1.2%-11.4%) for each unit increase in strain values. Conclusions The data from our study showed an anatomical correspondence between the areas of low potential identified by electroanatomical mapping and the areas of anomalous longitudinal strain. In particular, the endocardial strain correlates with the bipolar map, and the meso-epicardial strain correlates with the unipolar map. These data may prove useful in the appropriate planning of the procedure in patients with an indication for catheter ablation in the context of recurrent VT.

Implications for Paleontological Heritage Conservation: The Spatial Distribution and Potential Factors Controlling the Location of Fossil Sites of Shandong Province in China

Shandong Province in China is rich in paleontological fossils and has a long history of fossil research. However, research on the distribution characteristics and potential factors of discovered fossil sites in Shandong Province is limited and insufficient, making it difficult to comprehensively plan for the protection and utilization of fossil sites in Shandong Province. The study constructs a basic geographical information system (GIS) database with 133 discovered fossil sites and geological and socio-economic data of Shandong Province and studies fossil sites’ spatial distribution characteristics and the spatial relationship with potential factors at a regional scale. The results are as follows: (1) The fossil sites in Shandong Province are concentrated in the mountainous area of central Shandong and the hilly area of the Shandong Peninsula, with significant uneven distribution characteristics, including two agglomeration areas and seven sub-agglomeration areas. (2) Natural geographical conditions, such as topography, paleogeography, and stratigraphy, play a positive role in the distribution of fossil sites, and there are apparent concentrations in the following areas: at an altitude greater than 100 m; the Lower Paleozoic and Cretaceous sedimentary rocks; and the active areas of paleo-tectonics. (3) A certain degree of negative correlation exists between socio-economic conditions, such as roads and population density, and the number of fossil sites, and a positive correlation exists between disposable personal income and those fossil sites. The operational procedure presented here is a simple, objective, applicable method that can enhance our understanding of the spatial distribution patterns and influencing factors of the discovered fossil sites of Shandong Province and support more effective and appropriate planning for paleontological heritage conservation.

Peroxisome proliferator-activated receptor gamma regulates interleukin-6 induced lipoprotein(a) gene expression in human HepG2 cells

Abstract Background Lipoprotein(a)[Lp(a)] is an independent risk factor for atherosclerotic cardiovascular disease (ASCVD) and outcomes. The LPA gene promoter contains an interleukin 6 (IL-6) responsive binding site. IL-6 is a pro-inflammatory cytokine and data from patients with inflammatory diseases indicate an impact of the inflammatory milieu on LPA gene regulation and Lp(a) production. In a clinical study, the peroxisome proliferator-activated receptor gamma (PPARγ) agonist pioglitazone was associated with decreased Lp(a) levels. Publicly accessible ChIP-sequencing of human hepatocytes and measures of Lp(a) production by HepG2 cells provide an opportunity to explore PPARγ regulation of hepatic Lp(a) production. Purpose To investigate the role of PPARγ as a regulator of hepatic Lp(a) production. Methods Public ChIP-sequencing of human hepatocytes was analyzed. Commercially available HepG2 cells were incubated with one of the following: (1) scrambled small interfering RNA (siRNA) alone (control); (2) IL-6 (20ng/mL) and scrambled siRNA ; (3) IL-6 and PPARγ siRNA ; and (4) IL-6 and pioglitazone (25uM). LPA transcription was measured using the quantitative polymerase chain reaction. Results Analysis of the ChIP-sequencing data demonstrated that the LPA locus variant rs56393506, which is associated with increased Lp(a) levels and ASCVD risk in the general population, was within the location of the PPARγ binding site in the LPA promoter (Figure 1). IL-6 significantly increased LPA transcription in the HepG2 cells, compared to control (1.65±0.44 vs. 0.66±0.45, p=0.0027). In the presence of siRNA-mediated knock-down of PPARγ (58±17% PPARγ reduction, p<0.0001 vs. scrambled control), the effect of IL-6 on LPA transcription was significantly amplified (6.78±2.4 vs. 1.65±0.44, p=0.0009). IL-6 did not increase LPA transcription in the presence of the PPARγ agonist pioglitazone compared to control (0.16±0.03 vs. 0.66+0.45, p=0.11, Figure 2). Conclusion PPARγ is a negative regulator of IL-6 induced hepatic Lp(a) production and may represent a new therapeutic target in patients with inflammatory conditions.Figure 1Figure 2

Impact of the WELLBASED urban programs on health, well-being, and energy poverty

Abstract Background The WELLBASED project developed and implemented urban programs across six pilot sites to tackle energy poverty. This study aimed to evaluate these programs’ effectiveness on a broad range of health, well-being, and energy poverty indicators. Methods a controlled study was performed in six European pilot sites. Participants in the intervention condition receive the 12-month WELLBASED urban program. Participants were recruited via different methods and provided informed consent to participate in the 18-month study. Self-report questionnaires were completed at baseline, 6-, 12 and 18 months in both research conditions. In the intervention group, monthly health monitoring of e.g. heart rate and blood pressure, and continuous monitoring of indoor CO2, temperature and humidity were performed. Descriptive statistics and regression analyses are performed to assess the impact of the urban programs on health, well-being and energy poverty-indicators Results In the baseline sample (n = 1350; n = 661 intervention group, n = 689 control group) the mean age was 49 years (SD 16), 15.6% was >65 years, 65% were women, and 42% was married. At baseline people reported not feeling comfortable warm in winter (58%) or cool in summer (70%), 43% reported damp or leak. Preliminary findings at 6-month follow-up indicate changes in HR-QoL, subjective well-being and depression/anxiety differ strongly per pilot site location (p < 0.01). In summer 2024 all data will be available for analysis. Conclusions the comprehensive urban programs developed in the WELLBASED project may support public health to tackle energy and improve health and well-being of vulnerable citizens.

Advanced mapping and ablation technologies to target pulmonary veins gaps and residual antral potentials during repeat paroxysmal AF ablation: acute and long-term follow-up

Abstract Background A comprehensive understanding of pulmonary vein (PV) reconnection and residual potentials (RAP) within the antral scar during repeat atrial fibrillation (AF) ablation, facilitated by advanced mapping and ablation technologies, is currently lacking. Optimizing radiofrequency (RF) deliveries after failed PV isolation (PVI) may enhance ablation efficiency. Purpose This study aims to assess both the acute and long-term outcomes focusing on PV gaps (PVGs) and RAPs, utilizing advanced mapping and ablation technologies during repeat PV ablation in patients (pts) with paroxysmal AF. Methods Repeat AF-CHARISMA study, a prospective, single-arm cohort study, included consecutive pts from 13 centers undergoing repeat ablation of paroxysmal AF. The Lumipoint (Boston Scientific) map-analysis tool was used sequentially on each PV component. All patients were followed-up for at least 12 months after the procedure. The ablation endpoint was PVI and electrical quiescence in the antral region. Long-term outcome was the recurrence of AT/AF over follow-up. Results One-hundred pts were included (69% after failed RF procedure, 31% after failed cryoablation procedure). A total of 276 PVGs (192 after RF ablation, 2.8±1.8/pt; 84 after cryoablation, 2.7±1.9/pt, p=0.665 vs RF) and 44 RAPs (43 after RF ablation, 0.6±1.1/pt; 1 after cryoablation, 0.03±0.2/pt, p=0.002 vs RF) were detected. In the majority of cases (75%), only PVGs were present whereas, in 21%, both PVGs and RAPs were detected (2 pts had only RAPs). In 2 pts no PVG nor RAP were identified. PVGs were most common at anterior sites (103, 37.3%), followed by posterior sites (75, 27.2%) and carina sites (49, 17.8%). PVG location sites did not differ between prior ablation approach. Only 1 pt after cryoablation showed a RAP (at carina site), whereas the 44 RAPs after RF were fairly distributed across different sites. Acute procedural success was 100%, with all PVs successfully isolated and RAPs completely abolished in all study pts. After the blanking period, over a follow-up of 480±211 days, 18 pts (18%) suffered an AT/AF recurrence (time to recurrence: 245±80 days). Previous ablation approach (HR=0.66, 95%CI: 0.2 to 2.0 for previous cryoablation, p=0.46), number of PVGs (1.05, 0.8 to 1.3, p=0.67) or number of RAPs (0.41, 0.1 to 1.2, p=0.12) were not associated to AT/AF recurrence. No major complications or adverse events occurred. Conclusion A structured ablation workflow for repeat catheter ablation, incorporating consistent high-density mapping and improved diagnostic tools to guide the procedure, demonstrated safety and efficacy in treating paroxysmal AF irrespectively of previous ablation approach. Residual potentials within the antral scar were more frequently detected after RF ablation compared to cryoablation.

FreshLanDiv: A Global Database of Freshwater Biodiversity Across Different Land Uses

ABSTRACTMotivationFreshwater ecosystems have been heavily impacted by land‐use changes, but data syntheses on these impacts are still limited. Here, we compiled a global database encompassing 241 studies with species abundance data (from multiple biological groups and geographic locations) across sites with different land‐use categories. This compilation will be useful for addressing questions regarding land‐use change and its impact on freshwater biodiversity.Main Types of Variables ContainedThe database includes metadata of each study, sites location, sample methods, sample time, land‐use category and abundance of each taxon.Spatial Location and GrainThe database contains data from across the globe, with 85% of the sites having well‐defined geographical coordinates.Major Taxa and Level of MeasurementThe database covers all major freshwater biological groups including algae, macrophytes, zooplankton, macroinvertebrates, fish and amphibians.

Design an Agricultural Soil and Environment Monitoring System Based on IoT

One of the problems farmers face is the inability to make complete, real-time, and accurate observations of their farmland. The system proposed in this paper helps farmers to know the condition of farmland from anywhere and anytime by using a web-based application. The main objective of this prototype is to reduce the failure of the growth process of farming commodities by knowing the conditions inside and outside the soil with a total of 14 parameters. Internet of Things (IoT) technology is used to implement the prototype, which consists of Sensor Panels, Controllers, Message Broker, and Backend Service. All obtained data, created and tested in real-time, are displayed on the application. In addition to real-time data display, the system also includes monitoring history, alerts, and site location management.

Providing quality-assessed and standardised soil data to support global mapping and modelling (WoSIS snapshot 2023)

Abstract. Snapshots derived from the World Soil Information Service (WoSIS) are served freely to the international community. These static datasets provide quality-assessed and standardised soil profile data that can be used to support digital soil mapping and environmental applications at broad scale levels. Since the release of the preceding snapshot in 2019, refactored ETL (extract, transform and load) procedures for screening, ingesting and standardising disparate source data have been developed. In conjunction with this, the WoSIS data model was overhauled, making it compatible with the ISO 28258 and Observations and Measurements (O&M) domain models. Additional procedures for querying, serving and downloading the publicly available standardised data have been implemented using open software (e.g. GraphQL API). Following up on a short discussion of these methodological developments we discuss the structure and content of the “WoSIS 2023 snapshot”. A range of new soil datasets was shared with us, registered in the ISRIC World Data Centre for Soils (WDC-Soils) data repository and subsequently processed in accordance with the licences specified by the data providers. An important effort has been the processing of forest soil data collated in the framework of the EU-HoliSoils project. We paid special attention to the standardisation of soil property definitions, description of the soil analytical procedures and standardisation of the units of measurement. The 2023 snapshot considers soil chemical properties (total carbon, organic carbon, inorganic carbon (total carbonate equivalent), total nitrogen, phosphorus (extractable P, total P and P retention), soil pH, cation exchange capacity and electrical conductivity) and physical properties (soil texture (sand, silt and clay), bulk density, coarse fragments and water retention), grouped according to analytical procedures that are operationally comparable. Method options are defined for each analytical procedure (e.g. pH measured in water, KCl or CaCl2 solution, molarity of the solution, and soil / solution ratio). For each profile we also provide the original soil classification (i.e. FAO, WRB and USDA system with their version) and pedological horizon designations as far as these have been specified in the source databases. Three measures for “fitness for intended use” are provided to facilitate informed data use: (a) positional uncertainty of the profile's site location, (b) possible uncertainty associated with the operationally defined analytical procedures and (c) date of sampling. The most recent (i.e. dynamic) dataset, called wosis_latest, is freely accessible via various web services. To permit consistent referencing and citation, we also provide a static snapshot (in this case, December 2023). This snapshot comprises quality-assessed and standardised data for 228 000 geo-referenced profiles. The data come from 174 countries and represent more than 900 000 soil layers (or horizons) and over 6 million records. The number of measurements for each soil property varies (greatly) between profiles and with depth, this generally depending on the objectives of the initial soil sampling programmes. In the coming years, we aim to gradually fill gaps in the geographic distribution of the profiles, as well as in the soil observations themselves, this subject to the sharing of a wider selection of “public” soil data by prospective data contributors; possible solutions for this are discussed. The WoSIS 2023 snapshot is archived and freely available at https://doi.org/10.17027/isric-wdcsoils-20231130 (Calisto et al., 2023).

Dimensions of Posterior Implant Restorations Related to Intra-Coronal Cantilever and Occlusal Forces: An In Vitro Study.

The aim of the present study was to investigate the relation of the implant position to the implant crown and analyze the location and magnitude of bite force. The dimensions of 49 posterior implant-supported restorations were recorded in terms of: 1. implant diameter, 2. crown length, 3. crown width, 4. crown height, 5. occlusal surface of the restoration. The crown/implant height was measured and the distance between the implant center and the restoration center revealed the size of intra-coronal cantilevers. The occlusal force analysis determined the point and magnitude of loading. Correlation coefficient r was used for the statistical process and statistical control was carried out with the aid of t-test with minimum level of significance p < 0.05. - The mean crown-to-implant ratio was 0.9 and in short implants (< 10mm) the ratio was 1.28. - Intra-coronal cantilever extensions ranged from 2.1 mm to 6.35 mm depending on crown size. - Occlusal loading was identified in cantilever areas of the crown. - Implant center deviates from crown center in 71%-82% of the cases depending on site location. - Implants with non-ideal position may support a functionally occluding crown. Intra-coronal cantilevers are a critical factor to avoid clinical complications and should be taken under consideration both in the planning and fabrication phase of implant restorations.

Antibacterial effect of semiconductor laser radiation against the strains of S. aureus and P. aeruginosa, leading pathogens in osteomyelitis

Introduction The study of the antibacterial effect of photodynamic therapy against the leading pathogens of chronic osteomyelitis is one of the promising directions today.Purpose of the work was to evaluate the antibacterial effect against the strains of Staphylococcus aureus and Pseudomonas aeruginosa with the ALOD-01 laser system in the presence of photodithazine.Materials and methods The object of the study was 24-hour archival cultures of gram-positive and gram-negative microorganisms belonging to two taxa: Staphylococcus aureus (25923), Pseudomonas aeruginosa (27853). The antibacterial effect after the exposure to laser radiation in the presence of photodithazine on the microbial cells of the studied cultures was assessed by the absence of microorganism growth in the area of the light beam.Results Laser exposure in combination with photodithazine (concentration 0.5–1.0 mg/ml) on S. aureus for two minutes at 200–300 J achieved a bactericidal effect in the beam area. A bactericidal effect on the entire surface of the Petri dish was achieved with light exposure of 400 J for 5 minutes and a photodithazine concentration of 1.0 mg/ml. Laser exposure for 2 minutes in the presence of photodithazine at a concentration of 0.5 mg/ml and 1 mg/ml did not have an antibacterial effect on P. aeruginosa strains. Continuous growth of microorganisms was observed on the dish. Increasing the light dose and exposure time contributed to a decrease in the growth of microbial cells. A bactericidal effect was obtained only in the center of the dish in treating the bacterial suspension with photodithazine at a concentration of 5 mg/ml.Discussion The effectiveness of PDT depends on the type of microorganisms, the anatomical location of the infection site, as well as the properties of the photosensitizer and the laser used. Depending on the structure of the cell wall, different susceptibility of bacteria to photodynamic effects is observed.Conclusion S. aureus strains can be successfully photoinactivated using photodithazine. For P. aeruginosa strains, it was not possible to find a regime in which microbial cell growth was absent throughout the dish. The photodynamic reaction occurs only when adequate doses of light energy act on the photosentisizer in the presence of oxygen in the medium, while the photodynamic damage is local and the bactericidal effect is limited by the zone of light exposure.

Traceology suggests an unexpected use of antler cheekpieces from the Early Iron Age site at Gzin, Poland

Archaeological data confirm the widespread use of horse tack throughout the North European Bronze Age and the succeeding Hallstatt period in an inventory that included metal cheekpieces, phalerae, rein-knobs and other horse-related accessories. Similar usage has been assumed in the region of northern Poland, which has also furnished evidence for the use of horse gear accessories made of antler. This paper reports the results of traceological, isotopic and experimental research on three antler cheekpieces found at the Early Iron Age stronghold of Gzin in northern Poland and relates them further with archaeological and ethnographic patterns of horse exploitation. The results indicate that the artefacts from Gzin have rare comparanda and are isotopically consistent with the δ18Ow range of the site location, which can both be taken as evidence of their local origin. Moreover, traceological and experimental evidence from this study places the function and use of the analysed objects into a new light, undermining their use for horseback riding and suggesting that they were employed as cheekpiece coverings. Combined with the ethnographic record, ceramic iconography and archaeolinguistics, our findings fuel a discussion of deer valorisation during the Lusatian period in Poland, showing that the cheekpieces from Gzin may have served as elements of deer headgear.

SPATIAL VARIABILITY OF THE GEOTECHNICAL PROPERTIES OF SOILS: A CASE STUDY OF ELIZADE UNIVERSITY

Soil variability can alter the mechanical behaviour of foundations. It is therefore, necessary to conduct site investigations specific geotechnical analysis before any construction. This study evaluated selected engineering index and properties of soils at three different locations (sites) and depths withing Elizade University (EU), Ilara-Mokin. Five soil samples were collected from each of these locations and their engineering index and properties were determined. Statistical analysis namely Analysis of Variance (ANOVA) was utilised to determine the effects of location within the campus on the selected engineering properties. The mean and standard deviation (SD) of the engineering properties of soils collected within each site were also determined. The study revealed that plastic index (Pi), liquid limit (LL), moisture content (Mc), and plastic limit (Pl) were in the range of 4 to 32, 38 to 58.5, 11.6 to 29.04 %, 20 to 42 and respectively. The engineering index of the soil and engineering properties of the soil were significantly affected by the location with F14,42 equal to 2.592212, p was 0.008673 and F14, 42 equal to 3.210318 and p was 0.001719, respectively (which are less than 0.05). The high SD also showed that the soil properties have a wide range of values within same site, this was particularly so, in the case of the Atterberg’s limits, shear strength parameters and bearing capacities. The concluded that there is variability in the soil properties within the location.

A First-Passage Model of Intravitreal Drug Delivery and Residence Time-Influence of Ocular Geometry, Individual Variability, and Injection Location.

Standard of care for various retinal diseases involves recurrent intravitreal injections. This motivates mathematical modeling efforts to identify influential factors for ocular drug residence time, aiming to minimize administration frequency. We sought to describe the vitreal diffusion of therapeutics in nonclinical species frequently used during drug development assessments. In human eyes, we investigated the impact of variability in vitreous cavity size and eccentricity, and in injection location, on drug disposition. Using a first-passage time approach, we modeled the transport-controlled distribution of two standard therapeutic protein formats (Fab and IgG) and elimination through anterior and posterior pathways. Anatomical three-dimensional geometries of mouse, rat, rabbit, cynomolgus monkey, and human eyes were constructed using ocular images and biometry datasets. A scaling relationship was derived for comparison with experimental ocular half-lives. Model simulations revealed a dependence of residence time on ocular size and injection location. Delivery to the posterior vitreous resulted in increased vitreal half-life and retinal permeation. Interindividual variability in human eyes had a significant influence on residence time (half-life range of 5-7days), showing a strong correlation to axial length and vitreal volume. Anterior exit was the predominant route of drug elimination. Contribution of the posterior pathway displayed a 3% difference between protein formats but varied between species (10%-30%). The modeling results suggest that experimental variability in ocular half-life is partially attributed to anatomical differences and injection site location. Simulations further suggest a potential role of the posterior pathway permeability in determining species differences in ocular pharmacokinetics.

Potential Effect of Mangrove Ecosystem on Soil Carbon Sequestration and its Physico-Chemical Properties

The mangrove ecosystem is a natural wetland located in the Red Sea that extends 500 km on the Egyptian western coast. It has great potential to sequestrate soil organic carbon, reduce atmospheric CO2, and enhance soil hydro-physical properties. In this context, this research aims to characterise soil changes induced by mangrove growing. Both modelling and measurements herein were performed on five sampling areas at the Western Strand of the Red Sea (plus one control site - beach without plants). The locations (sites 1 and 2) of mangrove forest were in El-Gouna village with ages of 10 and 5 years, respectively, while (sites 3, 4, and 5) represent mangroves at Hurghada, Abu-Monquar Island, and Safaga, respectively. The mean values of measured soil organic carbon pool (SOCP) at the soil surface (0-90cm) revealed that the lowest values of the SOCP were at Hurghada with 8.81± 0.12 Mgha-1 and the highest values at Abu Monquar island with 59.75 ± 0.15 Mgha-1. At El Gouna 1, 2 and Safaga, the SOCP values were 14.48, 12.86, and 39.98 Mgha-1, respectively. In addition, the SOCP at the control site (beach without plants) was 6.62± 0.25 Mgha-1. Thus, the mangrove ecosystem has a great potential to sequestrate the soil's organic carbon and reduce atmospheric CO2. Soil bulk density (SBD) values varied at El-Gouna1, 2, and Hurghada from 1.63 to 1.75 g/cm³, while the lowest SBD values were observed at Abu-Monquar Island and Safaga with 1.31± 0.02, g/cm³ and 1.53 ± 0.05 g/cm³, respectively. SBD at the control site was 1.75± 0.05 g/cm³, which reflects the higher values. Morphological characteristics reveal that tree height results varied from 110-130 cm, 50-70 cm, 150-200 cm, 200-300 cm, and 200-270 cm for ElGouna1, ElGouna2, Hurghada, Abu-Manqar, and Safaga, respectively. Higher values of tree height, size index, and density are convenient, as are the lower values of SBD at Abu-Monquar and Safaga compared to other site locations. The soil-water HYDRUS-1D model revealed that the soil water storage capacity at Abu-Monquar was higher than the other samples. Thus, the joint use of modelling and measurements enabled deeper insight and a suitable characterisation of soil physicochemical changes induced by mangrove growth.