Site Of Fat Deposition Research Articles

Selection of candidate genes for differences in fat metabolism between cattle subcutaneous and perirenal adipose tissue based on RNA-seq

The site of fat deposition plays an important role in meat quality and body health. Biologically, the perirenal visceral fat (PF) and back subcutaneous fat (BF) are distinct. Angus and Simmental cattle (Bos taurus) were used as models. HE staining, triglyceride assay kit and RNA-seq were used to analyze the differences in tissue morphology and lipid accumulation, co-genes, and differentially expressed genes (DEGs) between the two tissues. According to the findings, BF has a smaller cell area and greater lipid deposition ability than PF. RNA-seq generated approximately 10.99 Gb of data in each library, and 23,472 genes were identified. The genes FABP4, ADIRF, and SCD that are related to adipose deposition were highly expressed in four tissues. There were 1678 DEGs and 1955 DEGs between BF and PF in Angus and Simmental cattle respectively. Gene Ontology function analysis identified several DEGs involved in metabolism. KEGG pathway analysis showed that four pathways related to fat metabolism were enriched. In the BF, seven genes (COL1A1, COL1A2, COL3A1, COL2A1, RXRA, C1QTNF7, and MOGAT2) were up-regulated. Five genes (ADRB3, ABHD5, CPT1B, CD36, LPIN1) were down-regulated. This study identified candidate genes that led to differences in fat metabolism, which could be useful in cattle breeding.

Lipid accumulation, oxidative stress and immune-related molecules affected by tributyltin exposure in muscle tissues of rare minnow (Gobiocypris rarus)

Tributyltin (TBT) is reported to induce adipogenesis in fish, which might affect nutritional qualities and health status. Muscle tissues account for the majority of body mass, and have been described as a major site of fat deposition and an immunologically active organ. Therefore, the present study aims to evaluate whether chronic exposures of TBT, at environmental concentrations of 1, 10 and 100 ng/L, affects lipid accumulation, oxidative stress and immune status in muscle tissues of rare minnow (Gobiocypris rarus). After 60 d of exposure, TBT increased contents of total lipid, total cholesterol, triglyceride and fatty acids in muscle tissues. Interestingly, TBT exposure disrupted fatty acid composition and increased contents of unsaturated fatty acids (such as eicosapentaenoic acid and docosahexaenoic acid) in muscle tissues, which might be a response to preserve membrane functions from TBT exposure. Meanwhile, the concentrations of hepatic fatty acid desaturase 2 (Δ6-desaturase) and stearoyl-CoA desaturase (Δ9-desaturase) were increased after TBT exposure, which might contribute the increase of unsaturated fatty acids. Furthermore, TBT increased muscle lipid peroxidation products, antioxidant enzymes (superoxide dismutase, catalase and glutathione peroxidase), and the expression of immune-related molecules (tumor necrosis factor alpha, interleukin 1 beta and nuclear factor kappa B) in muscle tissues. The disruption of TBT on the lipid accumulation, oxidative stress and immune-toxic effects in muscle tissues of fish might reduce nutritional qualities, and affect growth and health status, which might pose a constant and serious threat to fish and result in economic loss in aquaculture.

Relationships between insulin resistance and frailty with body composition and testosterone in men undergoing androgen deprivation therapy for prostate cancer.

While androgen deprivation therapy (ADT) has been associated with insulin resistance and frailty, controlled prospective studies are lacking. We aimed to examine the relationships between insulin resistance and frailty with body composition and testosterone. Case-control prospective study. Sixty three men with non-metastatic prostate cancer newly commencing ADT (n=34) and age-matched prostate cancer controls (n=29) were recruited. The main outcomes were insulin resistance (HOMA2-IR), Fried's frailty score, body composition by dual x-ray absorptiometry and short physical performance battery (SPPB) measured at 0, 6 and 12months. A generalised linear model determined the mean adjusted difference (95% CI) between groups. Compared with controls over 12months, men receiving ADT had reductions in mean total testosterone level (14.1-0.4nmol/L, P<0.001), mean adjusted gain in fat mass of 3530g (2012, 5047), P<0.02 and loss of lean mass of 1491g (181, 2801), P<0.02. Visceral fat was unchanged. HOMA2-IR in the ADT group increased 0.59 (0.24, 0.94), P=0.02, which was most related to the increase in fat mass (P=0.003), less to lean mass (P=0.09) or total testosterone (P=0.088). Frailty increased with ADT (P<0.0001), which was related to decreased testosterone (P=0.028), and less to fat mass (P=0.056) or lean mass (P=0.79). SPPB was unchanged. ADT is associated with increased insulin resistance and frailty within 12months of commencement, independently of confounding effects of cancer or radiotherapy. Insulin resistance appears to be mediated by subcutaneous or peripheral sites of fat deposition. Prevention of fat gain is an important strategy to prevent adverse ADT-associated cardiometabolic risks.

Active immunization of fatty acid translocase specifically decreased visceral fat deposition in male broilers

Lipid accumulation of avian adipocytes is mainly dependent upon the fatty acid transmembrane uptake process mediated by membrane proteins, such as fatty acid translocase (FAT/CD36), fatty acid transport protein 1, and caveolin-2. To examine the effects of FAT/CD36 on spatial-specific fat deposition, 60 broiler chickens were randomly allocated to 2 groups by sex. Each male or female group contained 2 subgroups (n = 14-15) inoculated by intramuscular injection with chicken FAT/CD36 or BSA (control) immunogens at 34, 49, and 63 d. The subcutaneous and visceral fat deposits were measured, as were levels of plasma triglyceride and free fatty acid. Serum antibody titer was measured by ELISA. The mRNA expression levels of fatty acid transport-related genes in the adipose tissue of the male broilers were investigated to reveal the relationships among various fatty acid transporters. The results showed that active immunization with FAT/CD36 could significantly decrease the visceral fat of the male broilers by up to 40%, but it had no effect on subcutaneous fat stores of male broilers or on either site of fat deposition in female broilers. The concentration of plasma free fatty acids increased in the experimental groups for both male and female broilers. After the FAT/CD36 immunization, very low density lipoprotein receptor mRNA expression was upregulated in both the subcutaneous and visceral fat of male broilers, whereas peroxisome proliferator-activated receptor γ, FAT/CD36, and acyl-CoA binding protein mRNA expression levels were upregulated only in the visceral fat of male broilers. These results indicated a novel role of chicken FAT/CD36 in fat deposition, with sex- and spatial-specific effects.

Adipogenic differentiation state-specific gene expression as related to bovine carcass adiposity1

Genetic regulation of the site of fat deposition is not well defined. The objective of this study was to investigate adipogenic differentiation state-specific gene expression in feedlot cattle (>75% Angus; <25% Simmental parentage) of varying adipose accretion patterns. Four groups of 4 steers were selected via ultrasound for the following adipose tissue characteristics: low subcutaneous-low intramuscular (LSQ-LIM), low subcutaneous-high intramuscular (LSQ-HIM), high subcutaneous-low intramuscular (HSQ-LIM), and high subcutaneous-high intramuscular (HSQ-HIM). Adipose tissue from the subcutaneous (SQ) and intramuscular (IM) depots was collected at slaughter. The relative expression of adipogenic genes was evaluated using quantitative PCR. Data were analyzed using the mixed model of SAS, and gene expression data were analyzed using covariate analysis with ribosomal protein L19 as the covariate. No interactions (P > 0.10) were observed between IM and SQ adipose tissue depots for any of the variables measured. Therefore, only the main effects of high and low accretion within a depot and the effects of depot are reported. Steers with LIM had smaller mean diameter IM adipocytes (P < 0.001) than HIM steers. Steers with HSQ had larger mean diameter SQ adipocytes (P < 0.001) than LSQ. However, there were no differences (P > 0.10) in any of the genes measured due to high or low adipose accretion. Preadipogenic delta-like kinase1 mRNA was greater in the IM than the SQ adipose tissue; conversely, differentiating and adipogenic genes, lipoprotein lipase, PPARγ, fatty acid synthetase, and fatty acid binding protein 4 were greater (P < 0.001) in the SQ than the IM depot. Intramuscular adipocytes were smaller than SQ adipocytes and had greater expression of the preadipogenic gene, indicating that more hyperplasia was occurring. Meanwhile, SQ adipose tissue contained much larger (P < 0.001) adipocytes that had a greater expression (P < 0.001) of differentiating and adipogenic genes than did the IM adipose tissue, indicating more cells were undergoing differentiation and hypertrophy. Adipogenic differentiation state-specific gene expression was not different in cattle with various phenotypes, but adipogenesis in the SQ and IM adipose tissues seems to occur independently.

Stem cells and metabolic diseases

Obesity is a metabolic disorder, which has been recognized as a global epidemic. It contributes to insulin resistance, the major cause of Type 2 diabetes, as well as to the development of other related diseases. Our basic premise is that a better understanding of how adult stem cells of the pancreas contribute to the maintenance of the pancreatic beta-cell pool against the increased metabolic demands associated with obesity may provide new therapeutic targets for treating diabetes. At the same time, if we knew more about the biology of adipocyte formation, maintenance and deposition in obese individuals, perhaps some control over the adipocyte tissue mass of these individuals would be facilitated and treatment of obesity would become available. Many investigations in the field are therefore aimed at describing how adipocyte stem cells function in the various sites of fat deposition and the extent to which these stem cells contribute to both brown and white adipocytes. Studies on the differentiation of human embryonic stem cells along the pancreatic and adipocyte lineages may therefore better inform approaches to these studies.

Relationships of Body Composition and Fat Partition with Body Condition Score in Serra da Estrela Ewes

Twenty eight non-lactating and non-pregnant adult Serra da Estrela ewes, ranging in body condition score (BCS) from 1 to 4 were used to study the relationships between BCS, live weight (LW), body composition and fat partition. Ewes were slaughtered and their kidney knob and channel fat (KKCF), sternal fat (STF) and omental plus mesenteric fat (OMF) were separated and weighed. Left sides of carcasses as well as the respective lumbar joints were then dissected into muscle, bone and subcutaneous (SCF) and intermuscular fat (IMF). The relationship between LW and BCS was studied using data from 1,396 observations on 63 ewes from the same flock and it was found to be linear. Regression analysis was also used to describe the relationships among BCS and/or LW and weights (kg) and percentages in empty body weight (EBW) of dissected tissues. The prediction of weights and percentages in EBW of total fat (TF) and of all fat depots afforded by BCS was better than that provided by LW. Only the weight of muscle and the percentage of bone in the EBW were more efficiently predicted by LW than by BCS. IMF represented the largest fat depot with a BCS of 1 and 2, whereas SCF was the most important site of fat deposition with a BCS of 3 and 4. Allometric coefficients for each fat depot in TF suggest that the fat deposition order in ewes from this breed is: IMF, OMF, SCF and KKCF. Results demonstrate that BCS is a better predictor than LW of body reserves in this breed and that LJ is a suitable anatomical region to evaluate BCS.

Effect of dietary vitamin A restriction on marbling and conjugated linoleic acid content in Holstein steers

To determine the effect of duration of dietary vitamin A restriction on site of fat deposition in growing cattle, 60 Holstein steers (BW = 218.4 +/- 6.55 kg) were fed a diet based on high-moisture corn, with 2,200 IU of supplemental vitamin A/kg of DM (control) or no supplemental vitamin A for a long (243 d; LR) or short (131 d; SR) restriction before slaughter at 243 d. The SR steers were fed the control diet for the first 112 d. Steers were penned individually and fed for ad libitum intake. Jugular vein blood samples for serum retinol analysis were collected on d 1, 112, and 243. Carcass samples were collected for composition analysis. Subcutaneous fat samples were collected for fatty acid composition. Fat samples from the i.m. and s.c. depots were collected to measure adipocyte size and density. Feedlot performance (ADG, DMI, and G:F) was not affected (P > 0.05) by vitamin A restriction. On d 243, the i.m. fat content of the LM was 33% greater (P < 0.05) for LR than for SR and control steers (5.6 vs. 3.9 and 4.2% ether extract, respectively). Depth of back-fat and KPH percentage were not affected (P = 0.44 and 0.80, respectively) by vitamin A restriction. Carcass weight, composition of edible carcass, and yield grade were similar among treatments (P > 0.10). Liver retinol (LR = 6.1, SR = 6.5, and control = 44.7 microg/g; P < 0.01) was reduced in LR and SR vs. control steers. On d 243, LR and SR steers had similar serum retinol concentrations, and these were lower (P < 0.01) than those of control steers (LR = 21.2, SR = 25.2, and control = 36.9 microg/dL). Intramuscular adipose cellularity (adipocytes/mm2 and mean adipocyte diameter) on d 112 and 243 was not affected (P > 0.10) by vitamin A restriction. Restricting vitamin A intake for 243 d increased i.m. fat percentage without affecting s.c. or visceral fat deposition, feedlot performance, or carcass weight. Restricting vitamin A intake for 131 d at the end of the finishing period appears to be insufficient to affect the site of fat deposition in Holstein steers.

Regional fat placement in physically fit males and changes with weight loss.

The abdomen is the principal site of fat deposition in men, and because abdominal fat is readily mobilized during exercise, the relative proportion of fat in the abdominal site may negatively correlate with the amount of regular physical activity, and even with physical fitness. This study presents data for regional fatness in 165 fit young men (U.S. Army Ranger candidates; initial body fat = 14.7 +/- 4.7%) assessed by dual-energy x-ray absorptiometry (DEXA), and for relative changes occurring following a 13% weight loss produced by a 1000 kcal.d-1 energy deficit over 8 wk. Fat-free mass was constant across quintiles of percent body fat; only fat mass was different (16.2 +/- 2.2 kg and 6.0 +/- 1.4 kg at upper and lower quintiles, respectively). Truncal fat accounted for about 41% of total body fat in all quintiles; only the proportion of fat distributed to the arms was significantly higher in the fattest quintiles of men. Among a group of less intensely trained soldiers with the same average fatness as the highest quintile of Ranger students (20%), relative fat distribution to the trunk approached 50% of the total fat. Following weight loss, Ranger students lost half of the fat in all regions assessed (legs, arms, and trunk). The only significant association between regional losses and initial fatness was a greater proportion of fat lost from the arms in the fattest Rangers. These data suggest a "fit fat" distribution in active young men in which fat remains in the arms and legs until extreme weight loss occurs and the metabolically more active abdominal fat approaches depletion.

Rate and site of fat deposition in pigs selected for components of efficient lean growth rate

Rates and sites of fat deposition were measured in a population of Large White pigs, after six generations of divergent selection on lean growth rate with ad-libitum (LGA) and restricted (LGS) feeding, lean food conversion (LFC) and daily food intake (DFI).

Rate and site of fat deposition in pigs selected for components of efficient lean growth rate

Rates and sites of fat deposition were measured in a population of Large White pigs, after six generations of divergent selection on lean growth rate with ad-libitum (LGA) and restricted (LGS) feeding, lean food conversion (LFC) and daily food intake (DFI).

Growth and metabolism in somatotropin-treated steers: II. Carcass and noncarcass tissue components and chemical composition

Carcass and noncarcass tissue compositional characteristics were determined in growing Hereford steers treated with daily subcutaneous injections (20.6 mg/d) of recombinantly derived bovine somatotropin (rBST) for 112 d. For carcass primal cuts, weights and rates of gain of bone, lean and total fat and site of fat deposition were not significantly affected by rBST treatments with the exception of a few tissues (loin total weight, flank total and lean weight and shank total weight). Lean to fat ratios, however, were greater (P less than .1) for the loin, flank, chuck and brisket. Weights and growth of individual muscles from the hindquarter were not affected by rBST administration. Weights and(or) average daily gains of the liver, kidneys, lungs and trachea and head were greater (P less than .05) in rBST-treated animals. Weights and (or) average daily gains were greater (P less than .1) in rBST-treated steers for water in the total body and carcass, for CP in the total body and noncarcass, and for ash in the total body, carcass and noncarcass. Ratios of CP to ether-extractable fat in the total body were greater (P less than .1) in rBST steers. These data indicate that rBST modified carcass lean and fat composition in cattle, but responses were modest compared to effects of somatotropin treatment of swine.

Correlated responses in body composition based on selection for different indicator traits in mice.

Correlated responses in whole-body composition were determined in 12-wk-old male mice from replicate lines selected for 12 generations for high (HF) or low (LF) epididymal fat pad weight as a percentage of body weight (EPID) and high (HL) or low (LL) hind carcass weight as a percentage of body weight. The HF and LF lines diverged (P less than .01) in body fat percentage (FAT) and subcutaneous depot fat by 93 and 71%, respectively, of the control line (RC) mean. EPID increased (P less than .01) proportionately more than FAT in the HF line; EPID decreased (P less than .01) proportionately less than FAT in LF. Protein, fat and water as a percentage of empty body weight showed negative correlated responses (P less than .01) due to selection for EPID, but lean body mass, body weight and body length had positive correlated responses (P less than .01). Correlated responses of fat-free protein and ash percentage were minor. Correlated responses in HL and LL were the mirror images of those in HF and LF, but they generally were of smaller magnitude. The results indicate that, although there are high positive genetic correlations between fat depots in mice, local control of lipogenesis and(or) lipolysis exists at different sites of fat deposition. Further, the lack of correlated responses in fat-free percentage of protein (and percentage of ash) suggests that additive genetic variances are low for these traits and(or) the genetic correlations of these traits with the selection criteria are low.

Genetic factors in the regulation of adipose tissue distribution.

This paper reviews current data concerning the role of the genotype in human variation in fat distribution and its contribution in regulating fat deposition in various regions of the body, primarily the lower trunk area. After statistical control over age, gender and total amount of subcutaneous fat, one finds an additive genetic effect of about 20-25% of remaining human variance in amount of lower trunk fat and in the relative proportion of lower trunk versus extremity fat. In spite of such a moderate genetic effect in fat distribution, the preferential site of fat deposition when exposed to chronic overfeeding is largely determined by one's genotype. Characteristics of regional adipose tissue metabolism and morphology are probably involved in mediating some of these genetic effects but other regulatory mechanisms are undoubtedly implicated.

Effects of selection for low backfat thickness in pigs on the sites of tissue deposition in the body

ABSTRACTThe effects of selection for low backfat thickness on tissue deposition in different body sites has been investigated in pigs. Eight castrated male and eight female pigs from each of the selection and control lines maintained at the University of Newcastle upon Tyne were used. One castrated male and one female from each line was killed at 15, 30, 45, 60, 75, 90, 105 and 120 kg live weight following ad libitum food consumption and fully dissected.There were only small effects of line on carcass composition, selected pigs having 120 g/kg more bone than controls at the mean side weight (P &lt; 0·001) and 40 g/kg more lean (NS). At the same mean weight of subcutaneous fat, selected pigs had thinner backfat than controls (approximately 3 mm) over m. longissimus at the last rib and over the shoulder but not at the mid-rump or mid-back positions. There appears to have been a slight shift in the sites of fat deposition from above m. longissimus in the loin towards the mid-line and rump as a result of selection, even though mid-rump, C and K measurements were all included in the selection index. However, there was no difference between the lines in the weight distribution of subcutaneous fat between six regions and so these shifts must have been very localized. There was no difference between lines in the relationship between P2 fat thickness and proportion of lean in the side.The effects of selection on the sites of deposition within tissues other than subcutaneous fat were small. In particular there was no evidence that selection has caused relocation of body fat from subcutaneous to the other sites.

South African fish products. XXXIII.—The Rock Lobster: the Composition of the Hepatopancreas Oil

AbstractThe main sites of fat deposition in the rock lobster are the hepatopancreas and the gonads. Data on the yield and characteristics of the oils from these organs are given. The composition of the saponifiable and unsaponifiable fractions of the hepatopancreas oil was determined. The component acids (weights per cent.) are: saturated acids C14 I.I. C16 10.3, C18 8.1, C20 4.2, C22 0.5; unsaturated acids C14 2.4 (‐2.0 H), C16 7.3 (‐2.1 H), C18 14.3 (‐2.9 H), C20 28.4 (‐6.5 H), C22 19.0 (‐8.3 H), C24 2.6 (‐2.1 H) and C26 1.8 (‐2 H). Cholesterol and α‐glyceryl ethers are the main components of the unsaponifiable fraction.