Test methods that use pushing forces to evaluate the maximal load capacities of carts in design standards require a flat, smooth and horizontal steel plate and thus do not take into account the real conditions of work. Resistive forces of a single wheel of a cart in a uniform rectilinear motion were measured using a unique test bench with five loads. Forty-four wheels were tested (varying diameters, treads and bearings) with one steel plate and four resilient floor coverings. Based on a linear mixed model, all the following results were significant (p < 0.05). Resistive forces were increased linearly with the load and depended on the characteristics of both the wheel and floor. These forces decreased as the diameter increased. They were important for wheels with sleeve bearings but decreased for cone ball bearings and precision ball bearings. Resistive forces depended on the material of the tread and were higher for solid rubber treads. In contrast, the hardness of the tread had little effect. Resistive forces strongly depended on the hardness of the base foam of resilient floor coverings: the softer the base foam, the higher the resistive forces. Test methods in design standards should be reviewed, using corrective forces based on these present results, to prevent musculoskeletal disorders.