ObjectivesThe aim of this study was to develop a rapid method for human neutrophil antigenâ2 (HNAâ2) genotyping and use it for fast screening for the HNAâ2 deficiency phenotype in the Chinese population.BackgroundHNAâ2 is encoded by the CD177 gene, and a single nucleotide polymorphism (SNP) at cDNA position 787 (CD177*787 A > T) creates a stop codon, which is responsible for the HNAâ2 deficiency phenotype. Recently, the SNP c.787A > T has been demonstrated to be the primary genetic mechanism for HNAâ2 deficiency.MethodsThis study focused on determination of CD177 SNP c.787A > T. We designed a set of alleleâ and sequenceâspecific primers (SSP), which were used to develop a oneâtube polymerase chain reaction (PCR) assay for HNAâ2 genotyping. The HNAâ2 genotype of a total of 2185 Chinese blood donors was determined.ResultsThe validation of this HNAâ2 genotyping assay was confirmed by sequencing analysis. The HNAâ2 two alleles, CD177*787A and 787T, can be simultaneously determined in a single tube PCR. Thirteen individuals with the CD177*787AT genotype were detected in this study. The CD177*787TT homozygous genotype was absent, suggesting that HNAâ2 deficiency is rare in the Chinese population.ConclusionsThe validated assay described in this study is a rapid and reliable method for HNAâ2 genotyping, which may be used for fast screening for HNAâ2 deficiency.