Single-sex Groups Research Articles

INTRASPECIFIC AGGRESSIVENESS OF THREE SPECIES OF GOBIIDAE FISH OF THE GENUS PONTICOLA ILJIN, 1927 IN LABORATORY CONDITIONS

The intensity of intraspecific aggressiveness of three species of gobiidae fish of the genus Ponticola was studied – Pinchuk’s goby Ponticola cephalargoides (Pinchuk, 1976), mushroom goby Ponticola eurycephalus (Kessler, 1874) and ratan goby Ponticola ratan (Nordmann, 1840). A different level of intraspecific aggressiveness is shown in the investigated fish species. Pinchuk’s goby showed the greatest aggressiveness – its single-sex and mixed groups behaved more aggressively than groups of mushroom goby and ratan goby. At the same time, indicators of the intensity of aggressiveness in the groups of mushroom goby and ratan goby did not show a statistically significant difference between these two fish species.

Surgical outcomes and complications associated with ovariectomy in the southern stingray Hypanus americanus.

Southern stingrays (Hypanus americanus) are relatively large rays that are common and popular in public aquariums because of their size and gentle nature. In aquariums, as well as in the wild, female southern stingrays are fecund. They have a short gestation cycle and can sustain multiple pregnancies each year, each culminating with 2-10 young. This reproductive rate could quickly outpace capacity in managed care and result in a ray surplus. To prevent overpopulation, many aquaria have resorted to single sex groups with a preference for female-only populations. This is an effective way to control population growth, but forces the maintenance of two separated populations of rays; for females this interrupts normal reproductive cycling and replaces it with a protracted non-pregnant condition. An additional consideration is development of reproductive disease in females which is recognized by an enlarged, misshapen, and congested ovary with an abundance of cystic structures and an enlarged uterus with a thickened wall that is often filled with histotroph despite a non-pregnant status. There are no effective long-lasting medical treatments for this type of reproductive disease and mortality is often the result. This report describes a surgical technique for ovariectomy in southern stingrays including outcomes and complications. Ovariectomy as a surgical method prevents unwanted reproduction and has the benefit of reducing reproductive pathologies commonly observed in southern stingrays as they age. Seven stingrays 1-5.2 years old and 42-83.5 cm disc width underwent ovariectomy. After anesthesia, the ovary and a small amount of epigonal was excised via a left para-lumbar incision. Four of the seven rays survived five or more years post-procedure. Two rays died acutely of coelomitis and one ray died of complications unrelated to the procedure. This report details a surgical procedure for ovariectomy in southern stingrays including outcomes, complications, and recommendations.

The hows and whys of gendered grouping practices in primary physical education in England

ABSTRACT Background: Single- and mixed-sex grouping have long been a focus of attention in physical education (PE) and are matters often at the fore of discussions about curriculum planning, teaching, and learning. Nonetheless, there remains little consensus or guidance on which approach (or combination of approaches) should be preferred in PE in primary and secondary schools in England. Further, while single- and mixed-sex grouping have been extensively researched in PE in secondary schools, hitherto there remains an absence of research examining these practices in PE in primary schools. This research sought to address gaps in the literature to build a stronger evidence-base for decisions about gendered grouping practices in PE. Specifically, the study was designed to provide a snapshot of current single- and mixed-sex grouping arrangements in primary PE in England. Method: Data were collected via an online survey which was administered to all state-funded mainstream and special schools providing for children in Key Stage 1 (aged 5–7) and/or Key Stage 2 (aged 7–11) in the North-East of England (917 at the time of study). A total of 254 surveys were completed giving a response rate of 27.7%. Results: The responses indicate that nearly all schools were using mixed-sex grouping for PE, with children typically remaining in their mixed-sex form class and taught the same curriculum activities by their class teacher. Notably, no schools were using single-sex grouping for all PE lessons, although some were teaching combinations of single- and mixed-sex classes across different year groups and/or different activities of the curriculum. The responses also challenge the traditional dichotomous representation of single- and mixed-sex grouping by showing that some schools were grouping children into smaller single-sex groups within mixed-sex PE classes, particularly in activities requiring bodily contact. Some schools were also organising children into smaller mixed-sex groups within mixed-sex PE classes to encourage boys and girls to work together and support one another in their learning. Reported reasons for current grouping arrangements reflected both pragmatic considerations and perceived educational benefits of particular approaches, including school timetabling and consideration of gender equity in curriculum provision in PE. Conclusion: The conclusion calls for further research to explore the efficacy of single- and/or mixed-sex grouping in supporting and meeting the needs of all children in primary PE. Relatedly, we also highlight the importance of intersectional perspectives being brought to single- and mixed-sex grouping debates to inform the development of more nuanced, evidence-based policies and practices that promote inclusion and celebrate the diversity of children. We further suggest a need for research and policy to reconsider the language used in grouping practices to reflect commitments to gender diversity and gender equity.

Individually or as a Team-The Immunological Milieu in the Lung Caused by Migrating Single-Sex or Mixed-Sex Larvae of Schistosoma mansoni.

While the lung is considered an efficient site for stopping the larvae of the acute Schistosoma spp. infection phase from migrating through extensive inflammatory responses in the surrounding tissues, little is known about these processes. To date, the highest resistance to infection has been achieved in experimental studies with radiation-attenuated cercariae immunization, which elicits a strong Th1/Th2 response in the lung and results in up to 80% protection. Based on our own studies demonstrating a systemic, unpolarized Th1/Th2 response resulting from infection with male or female Schistosoma mansoni, we hypothesize that this atypical immune response is already detectable during the pulmonary passage of parasite larvae. Therefore, we examined the immune milieu in the lungs of mice caused by migrating schistosome larvae, either male or female (single-sex groups) or male + female (bisexual control), 4 and 16 days after infection in bronchoalveolar lavage and lung tissue by flow cytometry, qPCR, and multiplex analyzes. Our results show only minor differences in the inflammatory profile between the single-sex groups but significant differences compared with the bisexual control group. Both single-sex infected groups have increased expression of inflammatory markers in lung tissue, higher numbers of cytotoxic T cells (day 4 post-infection) and more T helper cells (day 16 post-infection), compared with the bisexual control group. A single-sex infection, regardless of whether it is an infection with male or female cercariae, causes an immune milieu in the lung that is clearly different from an infection with both sexes. In terms of identifying therapeutic targets to achieve resistance to re-infection, it is of great scientific interest to identify the differences in the inflammatory potential of male or female and male + female parasites.

Impact of South Africa’s April 2022 floods on women and men’s lives and gender relations in low-income communities: A qualitative study

In 2022, South Africa faced devastating flooding which resulted in the loss of at least 425 lives and widescale destruction of property. Using qualitative methods, we describe the gendered impact of the floods on homes and lives of women and men from very low-income housing areas. We conducted 16 in-depth interviews with women, and eight single-sex narrative group discussions and with 35 women and 15 men from flood-affected areas. The women were research participants in a project that commenced prior to the floods, and the men were recruited for this study. The floods were described by many as ‘heart-breaking’, as neighbouring homes collapsed, children were swept away, and people known to them lost their lives. However, other participants asserted that the floods ‘didn’t affect them much’, before describing considerable, impact on their houses, families, possessions, neighbourhoods, jobs, and other aspects of their lives. After the initial struggle to secure their homes, and rescue family and possessions from the floods, participants were faced with extensive interruption to water supply and electricity, which severely exacerbated stress, especially for women. We describe how the impact of the flooding unfolded and largely followed the contours of gender relations, rather than disrupting them. Most women did not describe violence against women (VAW) as escalating after the floods, but for those who did, the pathway followed the impact of the floods on men’s access to the central tenets of successful masculinity, notably the provider/protector role, and lashing out response. Participants also emphasised that their lives continued much as before once the immediate aftermath of floods was past, and in so doing demonstrated significant resilience, which is not described in existing models of disaster impact on VAW.

Preclinical trials in Alzheimer's disease: Sample size and effect size for behavioural and neuropathological outcomes in 5xFAD mice.

5xFAD transgenic (TG) mice are used widely in AD preclinical trials; however, data on sample sizes are largely unaddressed. We therefore performed estimates of sample sizes and effect sizes for typical behavioural and neuropathological outcome measures in TG 5xFAD mice, based upon data from single-sex (female) groups. Group-size estimates to detect normalisation of TG body weight to WT littermate levels at 5.5m of age were N = 9-15 depending upon algorithm. However, by 1 year of age, group sizes were small (N = 1 -<6), likely reflecting the large difference between genotypes at this age. To detect normalisation of TG open-field hyperactivity to WT levels at 13-14m, group sizes were also small (N = 6-8). Cued learning in the Morris water maze (MWM) was normal in Young TG mice (5m of age). Mild deficits were noted during MWM spatial learning and memory. MWM reversal learning and memory revealed greater impairment, and groups of up to 22 TG mice were estimated to detect normalisation to WT performance. In contrast, Aged TG mice (tested between 13 and 14m) failed to complete the visual learning (non-spatial) phase of MWM learning, likely due to a failure to recognise the platform as an escape. Estimates of group size to detect normalisation of this severe impairment were small (N = 6-9, depending upon algorithm). Other cognitive tests including spontaneous and forced alternation and novel-object recognition either failed to reveal deficits in TG mice or deficits were negligible. For neuropathological outcomes, plaque load, astrocytosis and microgliosis in frontal cortex and hippocampus were quantified in TG mice aged 2m, 4m and 6m. Sample-size estimates were ≤9 to detect the equivalent of a reduction in plaque load to the level of 2m-old TG mice or the equivalent of normalisation of neuroinflammation outcomes. However, for a smaller effect size of 30%, larger groups of up to 21 mice were estimated. In light of published guidelines on preclinical trial design, these data may be used to provide provisional sample sizes and optimise preclinical trials in 5xFAD TG mice.

Assessing Grade 9 Female students’ Participation in Mixed and Single Sex Groups in ELT Classes with Reference to Beriso Dukale Secondary School

Assessing Grade 9 Female students’ Participation in Mixed and Single Sex Groups in ELT Classes with Reference to Beriso Dukale Secondary School

Peer mediation in play settings for minimally verbal students with autism Spectrum disorder.

Peer-mediated interventions (PMIs) are effective strategies to foster socialization of children with autism spectrum disorder (ASD) in natural settings. However, research examining the efficacy of peer mediation for students with ASD who have the greatest cognitive and language impairments remains limited. Additionally, previous studies essentially targeted communicative abilities of participants. To address this gap, the present study evaluated the effects of a play-based PMI on three socio-communicative skills (play, social engagement and imitation) of minimally verbal students with ASD who also have a comorbidity of intellectual disability (ID). Seven children with ASD attending ordinary school settings and 14 typically developing (TD) preschoolers participated. Seven single-sex groups were formed, and children played together during two 30 min weekly sessions. TD children were trained according to the principles of the integrated play group model. We used a multiple-baseline design across participants to measure the effects of the intervention on play skills, social engagement and motor imitation of students with ASD. Outcomes revealed an intervention effect for most of the participants, despite some variations across children. After the peer training, four children increased their duration of functional/symbolic play, six children improved their duration of interactive play and five children increased their rates of motor imitation. Concerning maintenance gains, inter-individual differences are also important. These findings suggest that a play-based PMI may be a feasible option for targeting inclusive education and improving socio-communicative skills of some minimally verbal students with ASD who also have an ID. However, variations across children invite further research to clarify how individual factors can moderate the effects of PMIs in children with ASD who are the most impaired.

Diversity and sex differences in rectal gland volatiles of Queensland fruit fly, Bactrocera tryoni (Diptera: Tephritidae).

Rectal gland volatiles are key mediators of sexual interactions in tephritid fruit flies. We used solid-phase microextraction (SPME) plus gas chromatography-mass spectrometry (GC-MS) and gas chromatography-flame ionization detection (GC-FID) to substantially expand rectal gland chemical characterisation of the Queensland fruit fly (Bactrocera tryoni (Diptera: Tephritidae); Qfly). The SPME GC-MS analysis identified 24 of the 30 compounds previously recorded from Qfly rectal glands, plus another 21 compounds that had not previously been reported. A few amides and fatty acid esters dominated the chromatograms of males and females respectively, but we also found other esters, alcohols and aldehydes and a ketone. The GC-FID analyses also revealed over 150 others, as yet unidentified, volatiles, generally in lesser amounts. The GC-FID analyses also showed 49 and 12 compounds were male- and female-specific, respectively, both in single sex (virgin) and mixed sex (mostly mated) groups. Another ten compounds were male-specific among virgins but undetected in mixed sex groups, and 29 were undetected in virgins but male-specific in mixed sex groups. The corresponding figures for females were four and zero, respectively. Most short retention time peaks (including a ketone and an ester) were male-specific, whereas most female-biased peaks (including five fatty acid esters) had long retention times. Our results indicate previously unsuspected diversity of rectal gland volatiles that might have pheromone functions in males, but far fewer in females.

Goitered Gazelle Gazella subgutturosa Responded to Human Disturbance by Increasing Vigilance Rather than Changing the Group Size.

Simple SummaryWild animals perceive nonlethal human activities as a potential threat, and they respond to human disturbances in a variety of ways: changes in group size and time-investment on vigilance are common behaviors. In China, nature reserves are generally divided into three functional zones: the core zone, the buffer zone, and the experimental zone, which represent low, medium, and high human disturbance levels, respectively. However, mining developments in the three zones pose threats to goitered gazelle living in the Kalamaili Nature Reserve, China. In this study, we found that human disturbances have larger effects on smaller-sized, single-sex groups rather than larger-sized, mixed-sex groups. Goitered gazelle spend more time being vigilant in the experimental zone than in the other two zones. In the experimental zone, a greater linear decrease with respect to group size was observed. Our results indicate that intensive human disturbances in the core and buffer zones brought goitered gazelle and humans closer together, which induced changes in vigilance and grouping patterns of this social species. Ultimately, this may have negative effects on the fitness and survival of the species.Nonlethal human disturbances have been confirmed to have a negative effect on wildlife in a variety of ways, including on behaviors. In many studies, the impact of human disturbances on gregarious species of wildlife is assessed through changes in their social organization and vigilance. In our study in the Kalamaili Nature Reserve, China, we wanted to estimate the impacts of differing levels of human disturbances on two different group types (mixed-sex and all-male) of goitered gazelle, Gazella subgutturosa, living in three functional zones (experimental, buffer, and core zones), which represent high, medium, and low human disturbance levels, respectively. In addition, we studied the time spent vigilant as a function of group size with different levels of human disturbances in the three zones. In general, mixed-sex gazelle groups were of similar sizes in the three different zones, while all-male groups slightly differed in their sizes between the experimental and buffer zones. This may indicate that human disturbances have varying effects on the different group types, with smaller-sized, single-sex groups being more significantly affected by human disturbances than larger-sized, mixed-sex groups. Goitered gazelle showed higher vigilance levels in the experimental zone than in the two other zones. A trend of decreasing vigilance varying linearly with group size was also found in the three zones, and the rate of decrease was higher in the experimental zone. Increased habitat fragmentation and human activities brought goitered gazelle and humans closer together in the core zone. Therefore, stopping mining activities and returning the reserve to a continuous habitat with fewer environmental disturbances is the best way to establish and protect a stable population of this endangered species of gazelle.

PSVI-2 Effect of Increasing the SID Tryptophan:Lysine Ratio and Dietary Energy Level on Growth Performance of Growing-Finishing Pigs

Abstract The objective of this study was to evaluate the effect of increasing the SID Tryptophan:Lysine ratio in growing-finishing and dietary energy level in finishing on growth performance of growing-finishing pigs (43.6 ± 0.85 kg to 131.7 ± 2.09 kg BW). A RCBD was used with a 2 × 2 factorial arrangement of treatments: A) Dietary Energy Level: Low Energy (3,294 kcal/kg ME) vs. High Energy (3,527 kcal/kg ME) and B) SID Tryptophan:Lysine Ratio: Low Try:Lys [16%] vs. High Try:Lys [21%]. Dietary Energy Level treatment was applied from 96 kg BW to end of study and SID Tryptophan:Lysine Ratio treatment was applied throughout the study period. Diets were based on corn-soybean meal and corn coproducts and formulated to meet or exceed the nutrient requirements (NRC, 2012) for each of 4 dietary phases. A total of 1,356 pigs were housed in single-sex groups of 34 pigs (10 replicates) at a floor space of 0.63 m2/pig. Pigs had ad libitum access to feed and water. Pen was the experimental unit and data were analyzed using R version 4.0.2, with the model accounting for the fixed effects of treatment, the interaction, and the random effect of replicate. There were no treatment interactions (P &amp;gt; 0.05). High Try:Lys compared with Low Try:Lys increased (P &amp;lt; 0.05) overall ADG but had no effect (P &amp;gt; 0.05) on ADFI or G:F (Table 1). The High Energy treatment reduced (P &amp;lt; 0.05) overall ADFI and increased (P &amp;lt; 0.05) overall G:F compared with the Low Energy treatment. In summary, pigs fed the elevated SID Tryptophan:Lysine level had increased ADG and feeding a diet with a higher dietary energy level improved feed efficiency.

Bacterial Keratitis: Similar Bacterial and Clinical Outcomes in Female versus Male New Zealand White Rabbits Infected with Serratia marcescens

ABSTRACT Purpose Females and males respond differently to a number of systemic viral infections. Differences between females and males with respect to the severity of keratitis caused by Gram-negative bacteria such as Serratia marcescens are less well established. Methods In this study, we injected female and male New Zealand White rabbit corneas with a keratitis isolate of S. marcescens and evaluated the eyes after 48 hours for a number of clinical and microbiological parameters. Results No statistical differences in bacterial burden and corneal scores were recorded between female and male rabbits although there was a non-significant trend toward a higher frequency of female rabbits demonstrating hypopyons. Conclusions This data suggests that for experimental bacterial keratitis studies involving Gram-negative rods, a single sex or mixed group of rabbit is sufficient for evaluating pathology and bacterial burdens. This will reduce the number of animals used for subsequent studies.

Parent-adolescent communication about sexual and reproductive health including HIV and STIs in Oman

ABSTRACT Parent-adolescent sexual and reproductive health (SRH) communication plays an important role in reducing sexual risk behaviour and preventing HIV. We sought to enhance limited information about the unique barriers to parent-adolescent SRH communication in the Arab states. This mixed-methods study, using a two-phase sequential explorative design, was conducted in 15 public secondary schools (with grades 5 to 10 students aged 10 to 15 years) in Oman. In phase one, 15 parents participated in two single-sex focus group discussions and two in-depth individual interviews. The qualitative data were analysed using thematic analysis. In phase two, a convenience sample of 250 parents completed a paper-based self-administered questionnaire. Findings showed that parents rarely communicated with their children about SRH concerns, including HIV. Barriers to more open parent-adolescent SRH communication include the fear that such discussion might encourage early sexual debut; feelings of shyness, discomfort and shame; associated socio-cultural taboos; gender mismatch; and lack of SRH knowledge. Findings suggest the need for parental SRH education and training and school based SRH education programmes to improve adolescent SRH. Experience developing these may be helpful to other countries with similar religious beliefs and socio-cultural traditions seeking to improve parent-adolescent SRH communication.

Enhancing efficacy of a dissonance-based obesity and eating disorder prevention program: Experimental therapeutics.

Test the hypothesis that the efficacy of a dissonance-based obesity/eating disorder prevention program, Project Health, could be enhanced by implementing it in single-sex groups and adding food response inhibition and attention training. Using a 2 × 2 factorial design, young adults (N = 261; Mage = 19.3, 79% female; 64% White) were randomized to (a) single-sex or (b) mixed-sex groups that completed food response inhibition and attention training or (c) single-sex or (d) mixed-sex groups that completed generic response inhibition and attention training with nonfood images. Preregistered primary outcomes (body fat, Body Mass Index [BMI]), eating disorder symptoms and other outcomes were assessed at pretest and posttest. For one preregistered primary outcome, body fat loss, there was a significant interaction between the two manipulated factors (d = -.28), as well as significant main effects for sex composition of groups (d = -.18) and food response inhibition and attention training (d = -0.17), with the largest body fat loss occurring for single-sex groups implemented with food response inhibition and attention training. Although the two manipulated factors did not significantly affect the other outcomes (including BMI, the other preregistered primary outcome), there was a significant reduction in eating disorder symptoms across the conditions (within participant d = -.78), converging with prior evidence that Project Health produced larger reductions in symptoms than educational control participants. Results suggest that implementing Project Health in single-sex groups with food response inhibition and attention training produced the largest body fat loss effects, as well as significant reductions in eating disorder symptoms, suggesting that efforts to disseminate this prevention program are warranted. (PsycInfo Database Record (c) 2021 APA, all rights reserved).

Social Behaviour in Zoo Bachelor Groups: A Case Study of Related South American Fur Seals.

Simple SummaryAppropriate management of social groups is one of the greatest challenges that face zoos and aquaria worldwide. All-male social groups provide an opportunity for facilities to house surplus males in groups which optimise their welfare whilst they are being retained for future use in breeding programmes. Here, we investigated social behaviour in a relatively poorly studied species, the South American fur seal (SAFS). Four individuals housed in a related group were studied over a 6-month period. The results showed that their social relationships changed over time, although the individuals always engaged in more positive than negative interactions. We recommend establishing baseline social behaviour profiles of individuals to enable long-term monitoring of SAFS social groups, as has been recommended in other species. This will enable enhanced understanding of South American fur seals and will contribute to the development of evidence-based social management guidelines for this species.Appropriate management of social groups is one of the greatest challenges that face zoos and aquaria worldwide. To facilitate breeding programmes, particularly in polygynous species, there is a need to house surplus males in bachelor groups, yet for pinnipeds, the social impact of this management strategy is unknown. The aim of this research was to enhance understanding of sociality in South American fur seals (SAFSs), with a particular focus on social dynamics in a related bachelor group, and consider implications for evidence-based management of this species in zoos. The subjects were four related male seals housed at Bristol Zoo Gardens. Social interaction and nearest neighbour data were collected between February and July 2019. Individuals engaged in both positive and negative social interactions. Positive interactions were more frequent than negative interactions, and no excessive negative interactions were observed. Temporal dynamics were observed in social relationships, and negative interactions did not increase with the onset of the breeding season. Reciprocity in dyadic relationships was variable across the study months, and nearest neighbours were not necessarily reflective of social partners. This research highlights the importance of longitudinal monitoring of social relationships and establishment of baseline social behaviour profiles to support evidence-based species management. We advocate that this research is extended, to further develop our understanding of SAFS social needs within zoo environments, to understand the differences between single-sex and mixed-sex groups and to identify the degree to which the extensive research conducted in other polygynous species (e.g., gorillas) is applicable in the social management of South American fur seals moving forwards.

PSVI-15 Effects of Dietary SID Lysine Restriction in the Growing Period and Number of Dietary Phases on Growth Performance of Growing-finishing Pigs

Abstract This study evaluated the effects of dietary SID lysine restriction in the growing period and number of dietary phases in the growing-finishing (GF) period on growth performance of GF pigs (32.7 ± 9.43 kg to 115.5 ± 8.18 kg BW). A RCBD (block = gender and start day) was used with a 3 × 2 factorial arrangement of treatments: 1) Dietary SID Lysine Level {Control [NRC (2012) recommendation]; Moderate Restriction [0.15% lower than Control]; Aggressive Restriction [0.30% lower than Control]} and 2) Number of Dietary Phases in GF (4 vs. 2). Lysine level treatments were applied from 32 to 64 kg BW. Diets were based on corn-soybean meal and corn coproducts, formulated to be isocaloric, and to meet or exceed recommendations of NRC (2012) for each phase, with the exception of SID lysine levels which were according to treatment. A total of 4,488 pigs were housed in single-sex groups of 34 pigs (22 replicates) with floor space of 0.63 m2/ pig. Pigs had ad libitum access to feed and water throughout and were weighed at start and end of the study; feeder additions were recorded. Pen was the experimental unit and data were analyzed using R version 3.6.2. The model accounted for the fixed effect of treatments, the interaction, and random effect of block. There were no treatment interactions (P &amp;gt; 0.05) and no effect (P &amp;gt; 0.05) of Number of Dietary Phases on overall growth performance. Decreasing dietary SID lysine linearly reduced (P &amp;lt; 0.05) overall ADG and ADFI with no effect (P &amp;gt; 0.05) on G:F. Restricting dietary SID lysine level to the extent used in this study reduced overall growth rate with no impact on feed efficiency.

The challenges and opportunities of conducting PhD participatory action research on sensitive issues: Young people and sexting

This paper presents the challenges and opportunities of using a participatory action research (PAR) methodology in a social science PhD exploring young people’s attitudes to sexting in Northern Ireland. Based upon a children’s rights approach, a Young People’s Advisory Group (YPAG) was created to seek advice on data collection activities and resources to be used with the participants of the research. Single-sex and mixed-sex focus group interviews were conducted in one youth club with 17 young people. PAR provides opportunities for young people to voice their views on matters affecting their lives, and supports the development of interpersonal, teamworking and research skills. Challenges of PAR included recruiting young people, gaining consent from young people and parents. This study highlights the importance of including young people as co-researchers. As a result of the method used, young people confirmed that Relationships and Sexuality Education (RSE) is inadequate.

Habituation of common eland (Taurotragus oryx) to intensive routine handling, and the effect of immunocastration thereon

The temperament of captive common eland (Taurotragus oryx) during handling is crucial for their management, as they have been identified as an ideal antelope for game meat farming, and are well-represented in captive antelope populations world-wide. Generally speaking, common eland are considered undomesticated, and thus maintain their natural prey instincts, making it necessary to habituate them to routine handling. Immunocastration can be implemented as part of the herd management strategy in mixed or single-sex eland groups, and might reduce aggressive behaviour of males by blocking the production of testosterone, thereby improving docility for ease of handling. Within this study, data was collected to determine the influence of routine handling and immunocastration on the temperament of common eland during handling. Twenty-nine common eland were divided into two groups: sub-adults (n = 15; ca. 2 years old; 182.9 ± 59.37 kg) and juveniles (n = 14; ca. 6 months old; 94.18 ± 24.76 kg), and they were routinely handled every two weeks over a four-month period (10 handling events). Within each age group, females, males, and immunocastrated males were present. Immunocastration treatment was administered during the second and fourth handling events (2 mL Improvac®/ animal). During handling, animals were individually driven through the raceway and finally restrained in a squeeze chute, where routine biological samples and measurements were collected. Faecal androgen metabolite (fAM) concentrations were measured for each event, for males only. Each animal was focally sampled for specific behaviours during the handling. A temperament score was obtained by observing the general state of the animal from the point of entering the raceway, until its exit from the squeeze chute. Generalized linear mixed models tested the influence of immunocastration, week, group, fAM concentration, and body weight on the temperament score. Immunocastration did not affect the temperament score, the general state in the squeeze chute, the order of entering the handling system, and the chute exit. However, the temperament score and the state in the squeeze chute improved overtime (with animals being less nervous). Moreover, animals with low temperament scores (calmer) entered the system earlier, and exited the system calmly over the repeated handling period. The results show that routine handling through a raceway and squeeze chute system leads to habituation, and improves the ease of handling in common eland. However, immunocastration did not affect the temperament of the male common eland during handling.

Short- and mid-term effects on performance, health and qualitative behavioural assessment of Romane lambs in different milk feeding conditions

The common practice of artificially rearing lambs from prolific meat breeds of sheep constitutes a welfare issue due to increased mortality rates and negative health issues. In this multidisciplinary study, we investigated the possible short- and mid-term advantages of artificially feeding fresh ewe's milk instead of commercial milk replacer on lambs' growth, health and welfare. Romane lambs were either separated from their mothers on D3 and fed with Lacaune ewes' milk (LAC, n = 13) or milk replacer (REP, n = 15), or they were reared by their mothers (MOT, n = 15). On D45, they were weaned, gathered in single-sex groups until the end of the study on D150. Lamb performance and biomarkers of overall health were assessed by measuring: growth, dirtiness of the perianal area, enteric pathogens in the faeces, total antioxidant status and redox status assessed by plasma reduced glutathione/oxidised glutathione ratio, and immune response after vaccination against chlamydiosis. As an exploratory approach, blood cell transcriptomic profiles were also investigated. Last, qualitative behaviour assessment (QBA) was performed as an integrated welfare criterion. Lacaune ewes' milk and REP never differed in their average daily gain but grew less than MOT lambs in the early suckling period and just after weaning. No effect was detected afterwards. On D30, LAC and REP lambs had lower total antioxidant and higher redox status than MOT lambs but did not differ among themselves. Lacaune ewes' milk and MOT had a cleaner perianal area than REP lambs on D21, while faecal pathogen infection did not vary between the treatment groups. After vaccination, LAC also had a stronger immune response on D90 compared to REP lambs. Transcriptome analysis performed on D150 showed differential gene expression, mainly in relation to inflammatory, immune and cell cycle response, between male lambs of the LAC group and those of the MOT and REP groups. Based on QBA, LAC lambs never differed from MOT lambs in their general activity and varied from REP only on D21; REP lambs were always more agitated than MOT lambs. In conclusion, artificial milk feeding impaired early growth rate, health and emotional state mainly during the milk feeding period and at weaning. Feeding artificially reared lambs with fresh ewe's milk partly mitigated some of the negative effects induced by milk replacer but without achieving the full benefit of being reared by the mother.

80 Effects of dietary standardized ileal digestible lysine level in the nursery period and number of dietary phases in the grow-finish period on wean-to-finish growth performance of pigs

Abstract The objective was to determine if feeding differing levels of dietary standardized ileal digestible lysine (SID Lys) during the nursery phase influenced the growth performance response to the number of dietary phases fed in the grow-finish period. A split-plot design was used with a 3×2 factorial arrangement of the following treatments: 1) SID Lys level nursery period: Control (SID Lys at requirement) vs. Restricted (0.2 g/kg SID Lys below Control) vs. Excess (0.1 g/kg SID Lys above Control; 2) Number of Dietary Phases in grow-finish: 3 vs. 5. Diets were formulated to meet NRC (2012) requirements, except for SID Lys during the nursery period. The study was carried out over the nursery [weaning (21±2 d; 5.8 ± 0.05 kg BW) to wk 9 post-weaning] and grow-finish [wk 9 to 19 post-weaning (112 ± 3.7 kg BW)] periods using 4,356 pigs housed in single-sex groups of 44 pigs, at a floor space of 0.30 m2/pig and 0.62 m2/pig in nursery and grow-finish periods, respectively. Data were analyzed using PROC MIXED of SAS; pen was the experimental unit. The model included fixed effects of SID Lys level (main plot), Number of Dietary Phases (split plot), their interaction, and random effects of block and block by SID Lys level interaction. There were no treatment interactions (P &amp;gt; 0.05). The Restricted Lys treatment reduced (P &amp;lt; 0.05) ADG and G:F in the nursery period compared to other treatments, but had no effect on these measurements in the grow-finish or wean-to-finish periods. There was no effect (P &amp;gt; 0.05) of the number of dietary phases on growth performance in grow-finish. In conclusion, feeding low levels of Lys in the nursery reduced nursery but not wean-to-finish growth performance; reducing the number of dietary phases in grow-finish from 5 to 3 had no impact on growth performance.