Single Fiber Bundle Research Articles

Improving correlation based super-resolution microscopy images through image fusion by self-supervised deep learning.

Super-resolution imaging is a powerful tool in modern biological research, allowing for the optical observation of subcellular structures with great detail. In this paper, we present a deep learning approach for image fusion of intensity and super-resolution optical fluctuation imaging (SOFI) microscopy images. We construct a network that can successfully combine the advantages of these two imaging methods, producing a fused image with a resolution comparable to that of SOFI and an SNR comparable to that of the intensity image. We also demonstrate the effectiveness of our approach experimentally, specifically on cell samples where microtubules were stained with ATTO647N and imaged using a confocal microscope with a single photon fiber bundle camera, allowing for the simultaneous acquisition of an image scanning microscopy (ISM) image and a SOFISM (ISM and SOFI) image. Our network is designed as a self-supervised network and shows the ability to train on a single pair of images and to generalize to other image pairs without the need for additional training. Our approach offers a flexible and efficient way to combine the strengths of correlation based imaging techniques along with traditional intensity based microscopy, and can be readily applied to other fluctuation based imaging modalities.

Biomechanics of annulus fibrosus: Elastic fiber simplification and degenerative impact on damage initiation and propagation

This study addresses three primary objectives related to lumbar intervertebral disc (IVD) biomechanics under ramping quasi-static loading conditions. First, we explore the conditions justifying the simplification of axisymmetric elastic fiber families into single fiber bundles through discretized strain energy functions. Simulations reveal that a concentration factor exceeding 10 allows for a consistent deviation below 10% between simplified and non-simplified responses. Second, we investigate the impact of elastic fibers on the physiological stiffness in IVDs, revealing minimal influence on biological motions but significant effects on degeneration. Lastly, we examine the initiation and progression of annulus fibrosus (AF) damage. Our findings confirm the validity of simplifying elastic fiber families and underscore the necessity of considering elastic fiber damage in biomechanical studies of AF tissues. Elastic fibers contribute to increased biaxial stretch stiffness, and their damage significantly affects the loading capacity of the inner AF. Additionally, degeneration significantly alters the susceptibility to damage in the AF, with specific regions exhibiting higher vulnerability. Damage tends to extend circumferentially and radially, emphasizing the regional variations in collagen and elastic fiber properties. This study offers useful insights for refining biomechanical models, paving the way for a more comprehensive understanding of IVD responses and potential clinical implications.

Deep convolutional generative adversarial network for generation of computed tomography images of discontinuously carbon fiber reinforced polymer microstructures

Computed tomography images are of utmost importance when characterizing the heterogeneous and complex microstructure of discontinuously fiber reinforced polymers. However, the devices are expensive and the scans are time- and energy-intensive. Through recent advances in generative adversarial networks, the instantaneous generation of endless numbers of images that are representative of the input images and hold physical significance becomes possible. Hence, this work presents a deep convolutional generative adversarial network trained on approximately 30,000 input images from carbon fiber reinforced polyamide 6 computed tomography scans. The challenge lies in the low contrast between the two constituents caused by the close proximity of the density of polyamide 6 and carbon fibers as well as the small fiber diameter compared to the necessary resolution of the images. In addition, the stochastic, heterogeneous microstructure does not follow any logical or predictable rules exacerbating their generation. The quality of the images generated by the trained network of 256 pixel ×\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$\ imes$$\\end{document} 256 pixel was investigated through the Fréchet inception distance and nearest neighbor considerations based on Euclidean distance and structural similarity index measure. Additional visual qualitative assessment ensured the realistic depiction of the complex mixed single fiber and fiber bundle structure alongside flow-related physically feasible positioning of the fibers in the polymer. The authors foresee additionally huge potential in creating three-dimensional representative volume elements typically used in composites homogenization.

The Influence of Glass Fiber and Copper Wire z-Binder on the Mechanical Properties of 3D Woven Polymeric Composites

AbstractThree-dimensional composites (3D) have potential applications in various fields due to their enhanced properties compared to conventional two-dimensional composites (2D). This study investigates the effect of different volumes of z-binder made from copper wire and E-glass fiber on the mechanical properties of 3D woven polymeric composites. The tensile, flexural, and fracture toughness behavior of four types of 3D orthogonal woven composites were studied in addition to a comparative 2D composite. The creation of the 3D orthogonal single-ply fabrics involved weaving z-binders using two different copper wire diameters, single fiber bundles, and double fiber bundles, each combined with four layers of woven E-glass fiber. The consolidation process for both 2D fabric and single-fabric 3D woven composites was executed using the hand lay-up technique. The results showed that most 3D woven composites outperformed 2D composites in terms of fracture toughness (stress intensity factor KIC and energy release rate GIC) and flexural strain. However, a decrease in flexural strength and tensile properties was observed for all 3D composites. The specimen with a small copper diameter had the smallest decrease of 5% in tensile strength. Furthermore, a decrease of 9% and 21% was attained by reinforcing with double and single glass fiber bundle z-binders, respectively, as compared with 2D composites. The highest enhancement of 92.5% in flexural failure strain was attained with double glass fiber bundles of z-binder. The maximum improvement in KIC fracture toughness, reaching 126% and 101.5%, was observed in specimens with a single glass fiber bundle z-binder and those with a large copper wire diameter, respectively.

Interaction Mechanism of Cementitious Composites Containing Different Twisted PVA Fiber Bundles

Fiber-reinforced cementitious composites (FRCCs) have made significant progress in improving the tensile and flexural properties of concrete members. Studies have shown that polyvinyl alcohol (PVA) fibers can effectively enhance the toughness of FRCCs, but the haphazard distribution of short-cut fibers makes it difficult to give full play to the high tensile strength of the fibers, and it is difficult for bundled fibers to effectively bond with the concrete substrate, which has become a challenge in the study of changing materials. Twisting the fibers by a physical process to improve the bonding performance of PVA fibers with FRCC substrate is a safe and feasible solution. However, research on silicate cementitious composites reinforced with twisted PVA fibers is limited. In this study, the interaction mechanism of through-length twisted PVA FRCCs with different twist factors and fiber bundle numbers was investigated. A concrete matrix material configured from silicate cement, fly ash, silica fume, and medium sand, in which PVA fibers with different twist factors were pre-incorporated, was used for the tests. Three-point bending tests were carried out on specimens with different twist factors (0, 50, 100, and 150 twists per meter) and fiber bundle numbers 1, 2, and 3. Compared to the untwisted PVA fiber specimens, the twist factor of 100 and the single fiber bundle specimens showed significant improvements in the bending properties, including a 36% increase in deflection, a 68% increase in the equivalent bending stress, and a 119% increase in energy consumption. Micro-X-ray computed tomography scans showed improved bending properties and energy consumption capabilities due to enhanced bonding properties as a result of the increased fiber–matrix interaction area and surface toughness.

The effect of fatty infiltration, revision surgery, and sex on lumbar multifidus passive mechanical properties.

Previous research has demonstrated increased stiffness in the multifidus muscle compared to other paraspinal muscles at the fiber bundle level. We aimed to compare single fiber and fiber bundle passive mechanical properties of multifidus muscle: (1) in 40 patients undergoing primary versus revision surgery and (2) in muscle with mild versus severe fatty infiltration. The degree of muscle fatty infiltration was graded using the patients' spine magnetic resonance images. Average single fiber and fiber bundle passive mechanical properties across three tests were compared between primary (N = 30) and revision (N = 10) surgery status, between mild and severe fatty infiltration levels, between sexes, and with age from passive stress-strain tests of excised multifidus muscle intraoperative biopsies. At the single fiber level, elastic modulus was unaffected by degree of fatty infiltration or surgery status. Female sex (p = 0.001) and younger age (p = 0.04) were associated with lower multifidus fiber elastic modulus. At the fiber bundle level, which includes connective tissue around fibers, severe fatty infiltration (p = 0.01) and younger age (p = 0.06) were associated with lower elastic modulus. Primary surgery also demonstrated a moderate, but non-significant effect for lower elastic modulus (p = 0.10). Our results demonstrate that female sex is the primary driver for reduced single fiber elastic modulus of the multifidus, while severity of fatty infiltration is the primary driver for reduced elastic modulus at the level of the fiber bundle in individuals with lumbar spine pathology.

Greater Importance of Structural Changes Over Lignin Removal in Impacting the Enzymatic Hydrolysis of Crop Straws.

The importance of structural changes and lignin removal in influencing the enzymatic hydrolysis of crop straws was investigated. Alkaline pretreatment of corn stover (CS) and rice straw (RS) was conducted to remove lignin. It was found that alkaline pretreatment caused more lignin removal from CS than RS under the same condition, and CS and RS treated at 40 °C (CS40 and RS40) attained less lignin removal than those treated at 80 °C (CS80 and RS80). However, CS40 achieved glucan conversion efficiency of 70.77% which was 11.85% less than that of RS40, and similar glucan conversion efficiencies were obtained for RS 40 and RS80. Structural observation showed that the connection among single fiber bundle of CS40 was tighter than that of RS40, while those of RS40 and RS80 were loose. It meant that structural changes might be more important than lignin removal to influence enzymatic hydrolysis of crop straws.

Structure and Physical Properties of Conductive Bamboo Fiber Bundle Fabricated by Magnetron Sputtering.

The variety of conductive fibers has been constantly enriched in recent years, and it has made rapid development in the fields of electronic textiles, intelligent wearable, and medical care. However, the environmental damage caused by the use of large quantities of synthetic fibers cannot be ignored, and there is little research on conductive fibers in the field of bamboo, a green and sustainable material. In this work, we used the alkaline sodium sulfite method to remove lignin from bamboo, prepared a conductive bamboo fiber bundle by coating a copper film on single bamboo fiber bundles using DC magnetron sputtering, and analyzed its structure and physical properties under different process parameters, finding the most suitable preparation condition that combines cost and performance. The results of the scanning electron microscope show that the coverage of copper film can be improved by increasing the sputtering power and prolonging the sputtering time. The resistivity of the conductive bamboo fiber bundle decreased with the increase of the sputtering power and sputtering time, up to 0.22 Ω·mm; at the same time, the tensile strength of the conductive bamboo fiber bundle continuously decreased to 375.6 MPa. According to the X-ray diffraction results, Cu in the copper film on the surface of the conductive bamboo fiber bundle shows the preferred orientation of (111) the crystal plane, indicating that the prepared Cu film has high crystallinity and good film quality. X-ray photoelectron spectroscopy results show that Cu in the copper film exists in the form of Cu0 and Cu2+, and most are Cu0. Overall, the development of the conductive bamboo fiber bundle provides a research basis for the development of conductive fibers in a natural renewable direction.

Endoscopic image-guided laser treatment system based on fiber bundle laser steering

A miniaturized endoscopic laser system with laser steering has great potential to expand the application of minimally invasive laser treatment for micro-lesions inside narrow organs. The conventional systems require separate optical paths for endoscopic imaging and laser steering, which limits their application inside narrower organs. Herein, we present a novel endoscopic image-guided laser treatment system with a thin tip that can access inside narrow organs. The system uses a single fiber bundle to simultaneously acquire endoscopic images and modulate the laser-irradiated area. The insertion and operation of the system in a narrow space were demonstrated using an artificial vascular model. Repeated laser steering along set targets demonstrated accurate laser irradiation within a root-mean-square error of 28 mum, and static repeatability such that the laser irradiation position was controlled within a 12 mum radius of dispersion about the mean trajectory. Unexpected irradiation on the distal irradiated plane due to fiber bundle crosstalk was reduced by selecting the appropriate laser input diameter. The laser steering trajectory spatially controlled the photothermal effects, vaporization, and coagulation of chicken liver tissue. This novel system achieves minimally invasive endoscopic laser treatment with high lesion-selectivity in narrow organs, such as the peripheral lung and coronary arteries.

Potential of Thai Bast Fibers for Injection Molded PLA Composites

Thailand has a huge variability of bast fiber plants, some of which have been little researched regarding their applicability in composites. Bast fiber(bundle)s from different species were investigated and incorporated into a polylactide (PLA) matrix by injection molding. Hemp and kenaf were used as well-studied fibers, while roselle, Fryxell and paper mulberry are less extensively characterized. Tensile strength, tensile modulus and interfacial shear strength (IFSS) of single fiber(bundle)s were highest for hemp, followed by kenaf, roselle, Fryxell and paper mulberry. Despite the lower tensile strength and IFSS of paper mulberry, the highest tensile strength was achieved for the paper mulberry/PLA composite followed by hemp/PLA. Scanning electron microscope (SEM) analyses showed that the single cells in paper mulberry fiber bundles, in contrast to the other fiber types investigated, were only partially bonded to each other, which explains the lower strength of the fiber bundles. The higher aspect ratio of fiber(bundle)s of paper mulberry in the PLA composite can explain the good composite characteristics. Apart from hemp, paper mulberry shows the best reinforcing effect in the PLA matrix and offers interesting potential for composite applications. Compared to neat PLA, the tensile strength could be increased by 24% and the tensile modulus by 54%.

A Biomimetic Basalt Fiber/Epoxy Helical Composite Spring with Hierarchical Triple-Helix Structures Inspired by the Collagen Fibers in Compact Bone.

The lightweight property of helical composite spring (HCS) applied in the transportation field has attracted more and more attention recently. However, it is difficult to maintain stiffness and fatigue resistance at the same time. Herein, inspired by collagen fibers in bone, a bionic basalt fiber/epoxy resin helical composite spring is manufactured. The collagen fibers consist of nanoscale hydroxyapatite (increases stiffness) and collagen molecules composed of helical amino acid chains (can increase fatigue resistance). Such a helical structure of intercalated crystals ensures that bone has good resistance to fracture. Specifically, we first investigated the effect of adding different contents of NS to basalt fibers on the stiffness and fatigue properties of an HCS. The results show that the optimal NS content of 0.4 wt% resulted in 52.1% and 43.5% higher stiffness and fatigue properties of an HCS than those without NS, respectively. Then, two braided fiber bundles (TS-BFB) and four braided fiber bundles (FS-BFB) were designed based on the helical structure of amino acid chains, and the compression tests revealed that the maximum load resistance of TS-BFB and FS-BFB was increased by 29.2% and 44%, respectively, compared with the conventional single fiber bundle (U-BFB). The superior mechanical performance of TS-BFB and FS-BFB is attributed to the more adequate bonding of 0.4 wt% NS to the epoxy resin and the multi-fiber bundles that increase the transverse fiber content of the spring. The findings in this work introduce the bionic collagen fiber structure into the design for an HCS and provide a new idea to improve the spring performance.

Understanding the Origin of Tensile Response in a Graphene Textile Strain Sensor with Negative Differential Resistance.

The flexible strain sensors based on the textile substrate have natural flexibility, high sensitivity, and wide-range tensile response. However, the textile's complex and anisotropic substructure leads to a negative differential resistance (NDR) response, lacking a deeper understanding of the mechanism. Therefore, we examined a graphene textile strain sensor with a conspicuous NDR tensile response, providing a requisite research platform for mechanism investigation. The pioneering measurement of single fiber bundles confirmed the existence of the NDR effect on the subgeometry scale. Based on the in situ characterization of tensile morphology and measurement, we conducted quantitative behavior analyses to reveal the origin of tensile electrical responses in the full range comprehensively. The results showed that the dominant factor in generating the NDR effect is the relative displacement of fibers within the textile bundles. Based on the neural spiking-like tensile response, we further demonstrated the application potential of the textile strain sensor in threshold detection and near-sensor signal processing. The proposed NDR behavior model would provide a reference for the design and application of wearable intelligent textiles.

Micro-biopsies: a less invasive technique for investigating human muscle fiber mechanics.

The purpose of this investigation was to demonstrate that muscle fiber mechanics can be assessed on micro-biopsies obtained from human medial gastrocnemii. Three micro-biopsy samples were collected from female dancers (n=15). Single fibers and fiber bundles were isolated and passively stretched from 2.4 to 3.0 µm at 0.015 and 0.04 µms-1 (n=50 fibers total) and in five increments at 0.12 µms-1 (n=42 fibers total). Muscle fibers were then activated isometrically at 2.4 µm (n=4 fibers total) and 3.0 µm (n=3 fibers total). Peak stress and steady-state stress were significantly greater (P<0.0001) after stretching at 0.04 µms-1 than at 0.015 µms-1. Furthermore, peak stresses and steady-state stresses increased non-linearly with fiber length (P<0.0001). We conclude that active and passive muscle fiber mechanics can be investigated using tissue from micro-biopsies.

Magnetically-sensitive nanodiamond thin-films on glass fibers

By assembling 140 nm-sized fluorescent nanodiamonds (FNDs) in a thin-film on (3-aminopropyl) triethoxysilane functionalized glass surface, we prepare magnetically-sensitive FND-fiber probes for endoscopy. The obtained FND layers show good uniformity over large surfaces and are characterized using confocal, fluorescence, and atomic force microscopes. Further, FNDs are assembled on single large-core multimode optical fibers and imaging fiber bundles end face to detect optically detectable magnetic resonance (ODMR) signals. The ODMR signals are recorded through the fiber’s far end in magnetic fields between 0 to 2.5 mT. A multi-channel sensor is demonstrated with the capability of parallel-in-time mapping and instantaneous readout from individual pixel and enabling magnetic mapping at high spatial resolution. Results of this study are promising for early stage detection in bio-diagnostic applications.

Tiny yet tough: Maximizing the toughness of fiber-reinforced soft composites in the absence of a fiber-fracture mechanism

Tiny yet tough: Maximizing the toughness of fiber-reinforced soft composites in the absence of a fiber-fracture mechanism

Number of fiber bundles in the fetal anterior talofibular ligament.

For the anterior talofibular ligament (ATFL), a three-fiber bundle has recently been suggested to be weaker than a single or double fiber bundle in terms of ankle plantarflexion and inversion braking function. However, the studies leading to those results all used elderly specimens. Whether the difference in fiber bundles is a congenital or an acquired morphology is important when considering methods to prevent ATFL damage. The purpose of this study was to classify the number of fiber bundles in the ATFL of fetuses. This study was conducted using 30 legs from 15 Japanese fetuses (mean weight, 1764.6 ± 616.9g; mean crown-rump length, 283.5 ± 38.7mm; 8 males, 7 females. The ATFL was then classified by the number of fiber bundles: Type I, one fiber bundle; Type II, two fiber bundles; and Type III, three fiber bundles. Ligament type was Type I in 5 legs (16.7%), Type II in 21 legs (70%), and Type III in 4 legs (13.3%). The present results suggest that the three fiber bundles of the structure of the ATFL may be an innate structure.

Proleg retractor muscles in Manduca sexta larvae are segmentally different, suggesting anteroposterior specialization.

Manduca sexta larvae are an important model system for studying the neuromechanics of soft body locomotion. They climb on plants using the abdominal prolegs to grip and maneuver in any orientation and on different surfaces. The prolegs grip passively with an array of cuticular hooks, and grip release is actively controlled by retractor muscles inserted into the soft planta membrane at the proleg tip. Until now, the principal planta retractor muscles (PPRMs) in each body segment were thought to be a single fiber bundle originating on the lateral body wall. Here, using high resolution X-ray microtomography of intact animals, we show that the PPRM is a more complex muscle consisting of multiple contractile fibers originating at several distinct sites on the proleg. Furthermore, we show that there are segmental differences in the number and size of some of these fiber groups which suggests that the prolegs may operate differently along the anterior-posterior axis.

On the Efficient Flow Restoration in Spectrally-Spatially Flexible Optical Networks

We focus on the efficient modeling and optimization of the flow restoration in the spectrally-spatially flexible optical networks (SS-FONs) realized using a single mode fiber bundle. To this end, we study a two-phase optimization problem, which consists of: (i) the network planning with respect to the spectrum usage and (ii) the flow restoration after a failure aiming at maximizing the restored bit-rate. Both subproblems we formulate using the integer linear programming with two modeling approaches—the node-link and the link-path. We perform simulations divided into: (i) a comparison of the proposed approaches, (ii) an efficient flow restoration in SS-FONs—case study. The case study focuses on the verification whether the spectral-spatial allocation may improve the restoration process (compared to the spectral allocation) and on the determination of the full restoration cost (the amount of additional resources required to restore whole traffic) in two network topologies. The results show that the spectral-spatial allocation allows us to restore up to 4% more traffic compared to the restoration with only the spectral channels. They also reveal that the cost of the full traffic restoration depends on plenty of factors, including the overall traffic volume and the network size, while the spectral-spatial allocation allows us to reduce its value about 30%.

Fracture Toughness of Three-Dimensional Stereolithography Printed Polymer Reinforced with Continuous Carbon Fibers.

Additive manufacturing of fiber-reinforced polymers is one of the latest technical developments in composites manufacturing. However, there is a severe shortage of research into continuous fiber-reinforced polymers manufactured through stereolithography. For the first time, this article investigates the fracture properties of continuous carbon fiber-reinforced polymer produced by three-dimensional stereolithography printing. Compact tension (CT) specimens, both plain and fiber reinforced, were produced and tested systematically. The results showed a significant improvement in fracture toughness for fiber-reinforced specimens when compared with plain ones. The positioning of fiber bundles had a substantial effect on fracture properties, and a higher fracture toughness was reported for specimens with the fiber bundle placed closer to the crack tip. By increasing the number of fiber bundles, a significant increase in fracture toughness was reported when compared with the sample with a single fiber bundle, indicating a strong contribution of fiber volume. Also, the contribution appeared to be most effective when the fiber bundles were placed symmetrically in the thickness direction. The study is of importance and value for the development of the stereolithography technique in manufacturing continuous fiber-reinforced composites with enhanced mechanical properties.

Study on intralaminar crack propagation mechanisms in single- and multi-layer 2D woven composite laminate

The intralaminar crack propagation of the carbon/epoxy two-dimensional plain woven composite material has been experimentally investigated. It was found that the propagation direction is insensitive to pre-crack tip locations. The propagation path direction is aligned along the intersecting position of warp fibers and weft fibers weaving, and the extends forward along the crack tip until the final fracture failure. The crack propagation process has two major stages, the maximum energy accumulation stage (stage A) and the gradual release of energy stage (stage B). In stage B, there are many single or several fiber bundles breakage during these stages due to the characteristics of reinforcing fibers woven from multiple fiber bundles. The warp fiber bundles gradually break under the axial tensile loading. Analysis of the images obtained by the optical microscope and scanning electron microscope, the fracture surfaces of the specimens are all sawtoothed, the fracture damage of the plain woven single-layer laminate is mainly fiber bundles separation, breakage of the fiber, and debonding of the fiber and the matrix. In addition, the validity of the intralaminar crack propagation mechanisms is further verified by multi-layer laminate experiments.