Single Dominance Research Articles

Peripheral activism: understanding the influence of California and Texas on the American Union

ABSTRACT A common conception of modern states is that central governments adopt and then enforce their desired laws on the entirety of their territory. The reverse, however, is also possible. That is, distant but prominent peripheries can influence the laws and functions of the entire territory. This article discusses the latter dynamic by considering the influence of California and Texas on US society. In the US, its member states can influence the centre vertically and horizontally. Vertical influence occurs when states directly influence the federal government – for California and Texas, this influence rests on their large number of federal representatives and their internal single party dominance. Horizontal influence occurs when states influence other states – that is, states can adopt successful laws already present in other states. A combination of the two can take place where states cooperate to then influence the centre. Together, these behaviours demonstrate peripheral activism, actions political sub-units take to stamp their preferred vision of society on their broader state. To supplement the discussion, I discuss the ideological divide between California and Texas and how they attempt to stamp their preferred vision of politics on the US.

REDEFINING MEDIA:

This article, based on changing trend of “mediamorphosis”, discusses an institutional viewpoint on media. It elaborates why a new definition to solve the problems of law making, drawing communication and media position in the state and society, and to liberate its users from the notion of single technology dominance in regulations is the need of the hour. It proposes that media may be envisaged as a process institution for transforming information into knowledge for the audience by creation and enlargement of message through instruments of process and delivery.

Constraining models of dominance for nonsynonymous mutations in the human genome.

Dominance is a fundamental parameter in genetics, determining the dynamics of natural selection on deleterious and beneficial mutations, the patterns of genetic variation in natural populations, and the severity of inbreeding depression in a population. Despite this importance, dominance parameters remain poorly known, particularly in humans or other non-model organisms. A key reason for this lack of information about dominance is that it is extremely challenging to disentangle the selection coefficient (s) of a mutation from its dominance coefficient (h). Here, we explore dominance and selection parameters in humans by fitting models to the site frequency spectrum (SFS) for nonsynonymous mutations. When assuming a single dominance coefficient for all nonsynonymous mutations, we find that numerous h values can fit the data, so long as h is greater than ~0.15. Moreover, we also observe that theoretically-predicted models with a negative relationship between h and s can also fit the data well, including models with h = 0.05 for strongly deleterious mutations. Finally, we use our estimated dominance and selection parameters to inform simulations revisiting the question of whether the out-of-Africa bottleneck has led to differences in genetic load between African and non-African human populations. These simulations suggest that the relative burden of genetic load in non-African populations depends on the dominance model assumed, with slight increases for more weakly recessive models and slight decreases shown for more strongly recessive models. Moreover, these results also demonstrate that models of partially recessive nonsynonymous mutations can explain the observed severity of inbreeding depression in humans, bridging the gap between molecular population genetics and direct measures of fitness in humans. Our work represents a comprehensive assessment of dominance and deleterious variation in humans, with implications for parameterizing models of deleterious variation in humans and other mammalian species.

Differential Trial-Type Effects in an Implicit Relational Assessment Procedure: Extending the DAARRE Model

The differential arbitrarily applicable relational responding effects (DAARRE) model explains two effects commonly observed with the implicit relational assessment procedure (IRAP): the single trial-type dominance effect (STTDE) and the dissonant-target trial-type effect (DTTTE). We sought to explore variables that modulate these effects. Forty-two participants who completed an IRAP met the practice criteria (median latency ≤ 2,000 ms and accuracy ≥ 80% correct) and thus proceeded to six test blocks of trials. The IRAP had four trial-types: trial-type 1: happy-face—happy-word; trial-type 2: happy-face—fear-word; trial-type 3: fear-face—happy-word; trial-type 4: fear-face—fear-word. Participants were randomly assigned to start with a consistent (respond True, False, False, True, respectively, to the four trial-types) or inconsistent (opposite responding) block. Difference (DIRAP) scores were calculated (inconsistent minus consistent latencies). A significantly larger DIRAP score was observed in trial-type 2 relative to trial-type 3 (a DTTTE), but only for participants who started with the inconsistent block. A “happiness superiority” STTDE (i.e., larger DIRAP score on trial-type 1 relative to trial-type 4) was observed, but only for participants who failed to maintain the criteria at the trial-type level (in any of the trial-types). Overall, the findings highlight the importance of distinguishing between the functional versus relational properties of the stimuli presented within an IRAP. The modulation of the DTTTE is interpreted in terms of functional coherence between the target and response option in inconsistent blocks. A novel data-analytic algorithm is presented to identify performance criteria violations at the trial-type level. The findings suggest that a greater focus is required in relational frame theory on the impact of the functional properties of stimuli on relational framing.

Double Outlet Right Ventricle with Non-Committed Ventricular Septal Defect and Other Complex Associated Anomalies in an Infant: Overview of the Surgical Management Approach

In the first place, the right ventricle is the primary or only point of origin for both main arteries in a patient with double outlet right ventricle (DORV). In order to guarantee proper repair, a wide variety of surgical approaches, considerations, and procedures are required for the wide spectrum of disorders that make up DORV. A functional classification based on physiological characteristics is one technique to classifying it, which might help determine the appropriate surgical strategy. Presentation of the case: Our case study focused on a female newborn infant who was 2 months old when she arrived at the hospital complaining of worsening shortness of breath and anorexia. The patient was diagnosed with pulmonary over-circulation, an abnormal accumulation of blood flowing into the lungs, through the following cardiac abnormalities: double outlet right ventricle (DORV) with a non-committal ventricular septal defect (VSD), no pulmonary stenosis (PS), mitral atresia, atrial septal defect (ASD), single ventricle dominance (SVD), anomalous pulmonary venous drainage (APVD), and patent ductus arteriosus (PDA). After a palliative procedure including the closure of the PDA and the implantation of a PA band, the patient was to have a surgical procedure known as univentricular repair. During the surgical meeting, this course of action was agreed upon. Conclusion: Different atrial configurations, atrioventricular connections, ventricular morphologies, and spatial interactions between the main arteries may all contribute to the wide anatomical spectrum of DORV. In order to decide between biventricular repair and univentricular palliation, several anatomical features must be present in the patient. In addition, palliative treatments may be necessary for certain patients before they may have a permanent surgical repair.

Surgical considerations and techniques using intraoperative indocyanine green angiography for ethmoidal dural arteriovenous fistula.

This study aims to investigate the efficacy of microsurgery with intraoperative indocyanine green (ICG) angiography as a treatment approach for ethmoidal dural arteriovenous fistula (DAVF). Between January 2010 and July 2021, our institution encountered a total of eight cases of ethmoidal DAVF. In each of these cases, microsurgical treatment was undertaken utilizing a bilateral sub-frontal interhemispheric approach, with the aid of intraoperative ICG angiography. ICG angiography identified bilateral venous drainage with single dominance in four cases (50%) of ethmoidal DAVF, a finding that eluded detection during preoperative transfemoral cerebral angiography (TFCA). The application of microsurgical treatment, in conjunction with intraoperative ICG angiography, resulted in consistently positive clinical outcomes for all patients, as evaluated using the Glasgow Outcome Scale (GOS) at the 6-month postoperative follow-up assessment; six patients showed GOS score of 5, while the remaining two patients attained a GOS score of 4. The use of intraoperative ICG angiography enabled accurate identification of both dominant and non-dominant venous drainage patterns, ensuring complete disconnection of the fistula and reducing the risk of recurrence.

Dominância da comunidade bacteriana em uma lagoa costeira eutrófica induzida por esgoto utilizando sequenciamento de nova geração: observações iniciais

Abstract: This study investigates the presence of bacterial dominance in one of the most studied sewage-driven eutrophic coastal lagoons, the Imboassica Lagoon in Macaé (RJ), Brazil, utilizing high-throughput sequencing of 16S rDNA. Water samples were collected from three sites within the lagoon. Total microbial DNA was extracted, and the V3-V4 region of the 16S rRNA gene was amplified and sequenced on the Illumina MiSeq platform. A total of 744,879 partial 16S rRNA sequences were clustered, revealing the absence of a single bacterial dominance in the sewage-driven eutrophic coastal lagoon. The prominent phyla detected in the lagoon were Cyanobacteria (27.8%), Proteobacteria (23.7%), and Actinobacteria (14.6%). Proteobacteria emerged as the most abundant phylum in the sewage-impacted lagoon site, whereas Cyanobacteria dominated the other two sampling sites. Among families, Synechococcaceae predominated with genus Synechococcus exhibited the highest prevalence. Families of potentially toxic Cyanobacteria represented less than 1% of the total families. The sewage-impacted lagoon section displayed greater bacterial diversity and richness. The dominance of bacterial communities associated with raw sewage, such as members of the Enterobacteriaceae family, was not confirmed, constituting only 0.75% of the families in the most affected site. This study presents the initial analysis of the bacterial community in the Imboassica Lagoon and suggests that dominance in the lagoon responds to the eutrophication and sewage discharge.

The Contribution of Non-Malay Architects to the Development of National Architecture Identity in Malaysia

The idea of adapting Malay traditional architectural values has been gazetted as one of the resolutions for National Architecture Identity as presented in ‘Seminar Ke Arah Identiti Kebangsaan Dalam Seni Bina’ in 1981. The resolution heavily influenced the adaptation of traditional Malay architecture, motives, and artefact onto modern architecture as a form to inculcate a singular and united national identity. A lot of research and documentation on good and meaningful architecture embracing the ideals of National architecture identity through the worldview and perspectives of Malay scholars and architects have been documented through books, journals, magazines and extensively discussed during seminars, symposiums, and discourses. Ironically, the contributions of Non-Malay architects adapting Malay symbolism to enunciate or articulate national pride and identity, either due to policies or interest, are minimally discussed and highlighted. This publication attempted to document the contributions of non-Malay architects to the national architecture identity construct. It is crucial to shape future philosophies, theories, criticisms, and thoughts on the overall built environment in developing a comprehensive construct of national architectural identity that does not necessarily rely on ethnocentric criteria based on a single race dominance. The research allows us to expand and progress the local architecture narrative by including diverse voices and thoughts, especially of those who have contributed extensively in the eras of nation-building. This inclusive and diverse input will, in turn, inform and expand the discourse of national identity and unity.

Quark-lepton Yukawa ratios and nucleon decay in SU(5) GUTs with type-III seesaw

We consider an extension of the Georgi-Glashow SU(5) GUT model by a 45-dimensional scalar and a 24-dimensional fermionic representation, where the latter leads to the generation of the observed light neutrino masses via a combination of a type I and a type III seesaw mechanism. Within this scenario, we investigate the viability of predictions for the ratios between the charged lepton and down-type quark Yukawa couplings, focusing on the second and third family. Such predictions can emerge when the relevant entries of the Yukawa matrices are generated from single joint GUT operators (i.e. under the condition of single operator dominance). We show that three combinations are viable, (i) yτ/yb=3/2, yμ/ys=9/2, (ii) yτ/yb=2, yμ/ys=9/2, and (iii) yτ/yb=2, yμ/ys=6. We extend these possibilities to three toy models, accounting also for the first family masses, and calculate their predictions for various nucleon decay rates. We also analyze how the requirement of gauge coupling unification constrains the masses of potentially light relic states testable at colliders.

New measurement of double- β decays of Mo100 to excited states of Ru100 with the CUPID-Mo experiment

The CUPID-Mo experiment, located at the Laboratoire Souterrain de Modane (France), was a demonstrator experiment for CUPID. It consisted of an array of 20Li2Mo100O4 (LMO) calorimeters, each equipped with a Ge light detector for particle identification. In this work, we present the result of a search for two-neutrino and neutrinoless double-β decays of Mo100 to the first 0+ and 2+ excited states of Ru100 using the full CUPID-Mo exposure (2.71kgyr of LMO). We measure the half-life of 2νββ decay to the 01+ state as T1/22ν→01+=(7.5±0.8(stat.)−0.3+0.4(syst.))×1020yr. The bolometric technique enables measurement of the electron energies as well as the γ rays from nuclear deexcitation and this allows us to set new limits on the two-neutrino decay to the 21+ state of T1/22ν→21+>4.4×1021yr(90% c.i.) and on the neutrinoless modes of T1/20ν→21+>2.1×1023yr(90% c.i.), T1/20ν→01+>1.2×1023yr(90% c.i.). Information on the electrons' spectral shape is obtained, which allows us to make the first comparison of the single and higher state dominance 2νββ decay models for the 01+ excited state of Ru100.8 MoreReceived 1 August 2022Accepted 2 December 2022DOI:https://doi.org/10.1103/PhysRevC.107.025503©2023 American Physical SocietyPhysics Subject Headings (PhySH)Research AreasDouble beta decayNeutrinoless double beta decayNuclear structure & decaysPhysical SystemsMajorana neutrinosNeutrinosProperties90 ≤ A ≤ 149Nuclear Physics

Viable quark-lepton Yukawa ratios and nucleon decay predictions in SU(5) GUTs with type-II seesaw

We investigate the viability of predictive schemes for quark-lepton Yukawa ratios and nucleon decay in non-supersymmetric SU(5) Grand Unified Theories (GUTs) where neutrino masses are generated by a type II seesaw mechanism. The scalar sector of the considered scenario contains 5-, 24- and 45-dimensional representations plus a 15-dimensional representation for realizing the type II seesaw. Predictions for the ratios of the quark and lepton Yukawa couplings emerge when the relevant entries of the Yukawa matrices are generated from single joint GUT operators (i.e. under the condition of single operator dominance). Focusing on the 2nd and 3rd family and hierarchical Yukawa matrices, we show that only two sets of predictions, yτyb=32, yμys=92 and yτyb=2, yμys=6 are viable. To further investigate both options, we extend the minimal scenarios to two “toy models”, including the 1st family of charged fermions, and calculate the models' predictions, e.g. for the nucleon decay rates and for the masses of the light relics that are potentially within reach of colliders.

Alternative stable ecological states observed after a biological invasion

Although biological invasions play an important role in ecosystem change worldwide, little is known about how invasions are influenced by local abiotic stressors. Broadly, abiotic stressors can cause large-scale community changes in an ecosystem that influence its resilience. The possibility for these stressors to increase as global changes intensify highlights the pressing need to understand and characterize the effects that abiotic drivers may have on the dynamics and composition of a community. Here, we analyzed 26 years of weekly abundance data using the theory of regime shifts to understand how the structure of a resident community of dung beetles (composed of dweller and tunneler functional groups) responds to climatic changes in the presence of the invasive tunneler Digitonthophagus gazella. Although the community showed an initial dominance by the invader that decreased over time, the theory of regime shifts reveals the possibility of an ecological transition driven by climate factors (summarized here in a climatic index that combines minimum temperature and relative humidity). Mid and low values of the driver led to the existence of two alternative stable states for the community structure (i.e. dominance of either dwellers or tunnelers for similar values of the climatic driver), whereas large values of the driver led to the single dominance by tunnelers. We also quantified the stability of these states against climatic changes (resilience), which provides insight on the conditions under which the success of an invasion and/or the recovery of the previous status quo for the ecosystem are expected. Our approach can help understand the role of climatic changes in community responses, and improve our capacity to deal with regime shifts caused by the introduction of exotic species in new ecosystems.

Nucleon decay in a minimal non-SUSY GUT with predicted quark-lepton Yukawa ratios

We investigate the predictions for various nucleon decay rates and their ratios in non-supersymmetric SU(5) Grand Unified Theories (GUTs) where the masses of the third and second family down-type quarks and charged leptons each stem dominantly from single GUT operators. Extending the Georgi-Glashow SU(5) model by a 45-dimensional GUT-Higgs representation, the gauge couplings can meet at the high scale MGUT and the GUT scale predictions yτ/yb=3/2 and yμ/ys=9/2 can emerge within single operator dominance. Explaining the observed neutrino masses via the type I seesaw mechanism by adding SU(5) singlet fermion representations and taking their renormalization group effects into account, we show that these predictions can lead to viable low scale second and third family down-type quark and charged lepton masses. To investigate nucleon decay predictions, we extend the minimal scenario to two “toy models” towards explaining quark and lepton masses and mixings with different flavor structure, and perform Markov Chain Monte Carlo (MCMC) analyses confronting the models with the available experimental data. We show that if several nucleon decay channels are observed, the ratios between their partial decay rates can serve as “fingerprints”, allowing to separate between GUT models with different flavor structure.

Dependence Structures between Sovereign Credit Default Swaps and Global Risk Factors in BRICS Countries

This study investigates the tail dependence structures of sovereign credit default swaps (CDSs) and three global risk factors in BRICS countries using a copula approach, which is popular for capturing the “true” tail dependence based on the “distribution-adjusted” joint marginals. The empirical results show that global market risk sentiment comoves with sovereign CDS spreads across BRICS countries under extreme market events such as the pandemic-induced crash of 2020, with Brazil reporting the highest bilateral convergence followed by China, Russia, and South Africa. Furthermore, oil price volatility is the second biggest risk factor correlated with CDS spreads for Brazil and South Africa, while exchange rate risk exhibits very low co-dependence with CDS spreads during extreme market downturns. On the contrary, exchange rate risk is the second largest risk factor co-moving with China and Russia’s CDS spreads, while oil price volatility exhibits the lowest co-dependence with CDS in these countries. Between oil price and currency risk, evidence of single risk factor dominance is found for Russia, where exchange rate risk is largely dominant, and policymakers could promulgate financial sector regulations that mitigate spill-over risks such as targeted capital controls when markets are distressed.

Analysis of Optimal Portfolio Formation Using Single Index Model and Stochastic Dominance on Sri-Kehati Index

This study directly applies the Single Index Model and Stochastic Dominance to solve the portfolio selection problem. This study aims to determine the difference in performance between the Single Index Model and Stochastic Dominance. The use of secondary data was used in this study by selecting the sample using the purposive sampling technique. If viewed based on the portfolio return, the single-index model can produce a portfolio return of (1.548%) and a stochastic dominance return of (0.888%). The results show that the value of the Stochastic Dominance portfolio formation has a Treynor index which is 2.22% higher than the Single Index Model with a Treynor index of 2.09%.

Spatiotemporal characteristics and influencing factors of urban resilience efficiency in the Yangtze River Economic Belt, China.

Urban resilience efficiency is an important indicator to explore the relationship between resource consumption and urban resilience, shedding new light on the study of urban sustainable development. Based on the panel data of 2008, 2012, and 2017, this paper makes a spatiotemporal assessment on the urban resilience efficiency of 126 cities in the Yangtze River Economic Belt (YREB) in China by applying an entropy weight-TOPSIS method and a slack-based measure (SBM) model. Combined with the analysis of a geographically weighted regression model (GWR), the influencing factors on resilience efficiency are also investigated. The results show that both the resource consumption index (RC, inputs) and the urban resilience index (UR, outputs) presented a steady upward trend, and their spatial distribution characteristics were similar, showing a gradual decrease from the eastern coastal cities to the central and western inland cities. Derived from inputs and outputs, the mean values of resilience efficiency index (RE) in three periods were 0.3149, 0.2906, and 0.1625, respectively, revealing that there had been a noticeable decline. Spatially, its spatial distribution has evolved from a relatively balanced pattern to an unbalanced one, showing a gradual decrease from west to east. The results of the GWR model analysis indicate that the total electricity consumption and area of construction land had a considerable correlation with the overall urban resilience of the YREB. Furthermore, total quantity of water supply and science and technology (S&T) expenditure continued to be the main driving factors on urban resilience of the upstream cities. The midstream regions mainly depended on the scale of construction land, and the influencing factors are relatively single. The influencing factors in the downstream areas have changed from dominance of resources and capital factors to the single dominance of resource factors, and total electricity consumption had a strong explanatory power. Based on these findings, we had put forward the overall and local regional policy implications.

Ready-made Garments, FDI and Total Exports of Bangladesh: A Time Series Analysis

This study examines the long-run and short-run causal relationships among readymade garments export, total export earnings, and net foreign direct investment inflows utilizing data from the financial year 1996-97 to 2019-20. The autoregressive distributed lag bounds test discloses the existence of a long-run relationship among the variables. This study also employed the Johansen cointegration test to supplement the results of the ARDL approach. This cointegration test confirms the presence of two cointegration equations. In addition, the Toda-Yamamoto test is performed to demonstrate the existence of short-run and long-run relationships among the variables. The results indicate that there is bidirectional causality between RMG exports and total exports in the short run as well as in the long run. Another important aspect of this study is that there is bidirectional causality between FDI and total export earnings in the short run but there is a unidirectional causality in the long run. The analysis also gives insights like single sector dominance, problems that existed in the RMG sector, and problems and prospects of FDI inflow. Further, keeping in view with these problems, the study urges pragmatic feasible measures for sustainable export growth.

Primary graft failure, but not relapse, may be identified by early chimerism following double cord blood unit transplantation

AbstractUmbilical cord blood transplantation (UCBT) has increased access to potentially curative therapy for patients with life-threatening disorders of the bone marrow and immune system. The introduction of reduced intensity conditioning (RIC) regimens and double umbilical cord unit infusions (DUCBT) has broadened the applicability of UCBT to more frail or larger recipients. The kinetics of chimerism after RIC DUCBT and their clinical utility are poorly understood. The RIC CBT trial reported here sought to prospectively evaluate the role of lineage-specific chimerism after DUCBT in adult patients with hematologic malignancies in the United Kingdom. Fifty-eight patients with a median age of 52 years were recruited, with overall and progression-free survivals of 59% (95% confidence interval [CI], 45%-71%) and 52% (95% CI, 39%-64%), respectively, at 2 years. Nonrelapse mortality was 4% (95% CI, 1%-13%) at day 100, and the relapse rate was 31% (95% CI, 21%-45%) at 1 year. Peripheral blood lineage-specific chimerism was feasible from day 7 after transplant onward. Five patterns of chimerism were observed including (1) complete single unit dominance (39 patients), (2) sustained donor-donor mixed chimerism (3 patients), (3) sustained donor-recipient mixed chimerism (5 patients), (4) dominance reversion (1 patient), and (5) primary graft failure (4 patients). The RIC CBT trial enabled adult patients with high-risk hematologic malignancies to safely access UCBT in the United Kingdom and provided novel insights into the kinetics of donor and recipient chimerism after RIC DUCBT that are clinically relevant. This trial was registered at https://www.clinicaltrialsregister.eu/ctr-search/trial/2004-003845-41/GB as #NCT00959231 and EudraCT 2004-003845-41.

Fragmentation in trader preferences among multiple markets: market coexistence versus single market dominance.

Technological advancement has led to an increase in the number and type of trading venues and a diversification of goods traded. These changes have re-emphasized the importance of understanding the effects of market competition: does proliferation of trading venues and increased competition lead to dominance of a single market or coexistence of multiple markets? In this paper, we address these questions in a stylized model of zero-intelligence traders who make repeated decisions at which of three available markets to trade. We analyse the model numerically and analytically and find that the traders’ decision parameters—memory length and how strongly decisions are based on past success—make the key difference between consolidated and fragmented steady states of the population of traders. All three markets coexist with equal shares of traders only when either learning is too weak and traders choose randomly, or when markets are identical. In the latter case, the population of traders fragments across the markets. With different markets, we note that market dominance is the more typical scenario. Overall we show that, contrary to previous research emphasizing the role of traders’ heterogeneity, market coexistence can emerge simply as a consequence of co-adaptation of an initially homogeneous population of traders.

Visualizing Clonal Hematopoiesis Associated with gata2 Deficiency in Zebrafish Using Color-Barcoding

Myeloid neoplasms, such as myelodysplastic syndromes (MDS) and acute myeloid leukemia (AML), arise from abnormal hematopoietic stem and progenitor cells (HSPCs) with acquired somatic mutations. Germline GATA2 haploinsufficiency is associated with an autosomal dominant familial MDS/AML syndrome. Leukemic transformation in GATA2 haploinsufficiency occurs with acquisition of secondary mutations or cytogenetic abnormalities. We generated gata2 heterozygous zebrafish to model this germline predisoposition syndrome, and evaluate clonal architecture and dynamics over time at steady-state and in the presence of additional mutations. We hypothesized that clinically benign clonal hematopoiesis precedes the establishment of myeloid neoplasms in GATA2 haploinsufficiency. We used a lineage tracing system of zebrafish Brainbow, Zebrabow, to track the hematopoietic output of individually colored HSPC clones, with the goal to detect abnormal expansion of single color clones in the early stages of leukemogenesis, indicating clonal hematopoiesis. A heterozygous line of GATA2 zebrafish ortholog gata2b was generated in the Zebrabow background. We introduced combinations of genetic mutations described in GATA2 associated MDS and AML into Zebrabow embryos with CRISPR/Cas9 technology. Zebrafish embryos, either wild-type or heterozygous for gata2b, were injected with Cas9 protein and guide RNAs targeting zebrafish orthologs of ASXL1 and STAG2. Zebrabow color recombination was induced to color-label stem cells prior to their emergence from the dorsal aorta. At this time roughly 20 stem cell clones exist in the zebrafish embryo. The animals were then raised to adulthood. Kidney marrow cells of juvenile zebrafish were analyzed at various time points in between 2-4 months post-fertilization (mpf) by flow cytometry and morphology on cytospins. At steady-state, juvenile gata2b+/- zebrafish had a decreased myeloid compartment in the kidney marrow. A third of gata2b+/- zebrafish also had an expansion of single color clones in the kidney marrow by 3 mpf, contributing to ≥30% of granulocytes, while no wild-type clutchmate demonstrated single color expansion to this degree at steady-state. Mosaic combinations of mutations in the presence of gata2b deficiency resulted in an increase of precursor and a more pronounced decrease of myeloid populations in the kidney marrow at 3 mpf, compared to wild-type zebrafish injected with same mutations. No changes were observed in the lymphoid compartment. Single color expansion was observed in gata2b+/- zebrafish three times more frequently than in wild-type zebrafish. In summary, gata2b deficiency sensitizes juvenile zebrafish to developing myelocytopenia at steady-state, reminiscent of well-characterized monocytopenia in GATA2 deficiency. We found that myelocytopenia is more profound and associated with an increase in early precursors upon additional somatic mutations associated with MDS/AML, possibly due to a differentiation block in myelopoiesis. Single color dominance observed in the hematopoeitic compartment of juvenile gata2b+/- zebrafish using the Zebrabow color-barcoding system presents a potential mechanism underlying the predisposition to myeloid neoplasms associated with GATA2 deficiency, and provides a tool for visualizing asymptomatic premalignant state in disease pathogenesis. DisclosuresZon:Scholar Rock, Inc: Consultancy, Equity Ownership; Marauder, Inc.: Consultancy, Equity Ownership; Stemgent: Consultancy; Fate, Inc.: Consultancy, Equity Ownership.