Single Crossover Research Articles

Impact of GnRH antagonist pretreatment on oocyte yield after ovarian stimulation: A retrospective analysis.

The study investigates whether a 3-day pretreatment course with a GnRH antagonist in the early follicular phase has an impact on the number of retrieved COCs in a GnRH antagonist stimulation protocol. This is a retrospective single center crossover study involving women who did not conceive after one GnRH antagonist stimulation cycle ("standard cycle") and proceeded with another GnRH antagonist stimulation cycle preceded by early administration of GnRH antagonist for 3 days ("pretreatment cycle") with fresh embryo transfer or frozen embryo transfer. 430 patients undergoing 860 cycles were included. The mean female age was 34.4 ± 4.8 years. Indications for fertility treatment included unexplained infertility (34.3%), male-factor infertility (33.3%), age (16.9%), PCOS (8.2%), tubal (4.7) and endometriosis (2.6%). All cycles were divided into two groups: group 1 (standard, 430 cycles) and group 2 (pretreatment, 430 cycles). The mean duration of stimulation was similar in both groups (10.3 vs 10.3 days, p = 0.28). The starting dose of gonadotropin (234.9 vs 196.8 IU, p<0.001), total amount of gonadotropin used (2419 vs 2020 IU, p<0.001), the total number of retrieved COCs (10 vs 7.8 p<0.001) and the number of mature oocytes (8 vs 5.8 p<0.001) were significantly higher in group 2 than in group 1. The Generalized estimating equation (GEE) regression analysis showed that the pretreatment strategy had a significant positive effect on the number of COCs (coefficient 2.4, p <0.001 after adjusting for known confounders (age, indication, stimulation dose, type, and duration of stimulation). In conclusion, A 3-day course of GnRH antagonist pretreatment increases the number of COCs obtained after ovarian stimulation.

Upgraded one-dimensional code for the design of a micro gas turbine mixed flow compressor stage with various crossover diffuser configurations

Abstract Micro Gas Turbine (MGT) designers often have the need for a design tool, capable of rapidly providing feasible design options. The upgrade of such an existing MATLAB® based mixed flow compressor design application (1D App) is discussed. In MGT engines, diffuser design largely influences the compressor stage performance. Crossover diffusers provide superior efficiency and pressure ratio performance compared to legacy type diffusers, albeit at the expense of operating range. The reduced operating range attributed to single vane crossover diffusers can be alleviated by splitter vane, tandem vane, and combined configurations. Therefore, the 1D App is updated to allow the user the ability to design these additional crossover diffuser configurations. Performance results are verified using Numeca/FINE™ Turbo CFD software. It is shown that the upgraded 1D App provides predicted performance results within 1 % of CFD results for both total-to-total efficiency and pressure ratio.

Length-sensitive partitioning of Caenorhabditiselegans meiotic chromosomes responds to proximity and number of crossover sites

Sensing and control of size are critical for cellular function and survival. A striking example of size sensing occurs during meiosis in the nematode Caenorhabditis elegans. C.elegans chromosomes compare the lengths of the two chromosome "arms" demarcated by the position of their single off-center crossover, and they differentially modify these arms to ensure that sister chromatid cohesion is lost specifically on the shorter arm in the first meiotic division, while the longer arm maintains cohesion until the second division. While many of the downstream steps leading to cohesion loss have been characterized, the length-sensing process itself remains poorly understood. Here, we have used cytological visualization of short and long chromosome arms, combined with quantitative microscopy, live imaging, and simulations, to investigate the principles underlying length-sensitive chromosome partitioning. By quantitatively analyzing short-arm designation patterns on fusion chromosomes carrying multiple crossovers, we develop a model in which a short-arm-determining factor originates at crossover designation sites, diffuses within the synaptonemal complex, and accumulates within crossover-bounded chromosome segments. We demonstrate experimental support for a critical assumption of this model: that crossovers act as boundaries to diffusion within the synaptonemal complex. Further, we develop a discrete simulation based on our results that recapitulates a wide variety of observed partitioning outcomes in both wild-type and previously reported mutants. Our results suggest that the concentration of a diffusible factor is used as a proxy for chromosome length, enabling the correct designation of short and long arms and proper segregation of chromosomes.

Direct computations of viscoelastic moduli of biomolecular condensates.

Biomolecular condensates are viscoelastic materials defined by time-dependent, sequence-specific complex shear moduli. Here, we show that viscoelastic moduli can be computed directly using a generalization of the Rouse model that leverages information regarding intra- and inter-chain contacts, which we extract from equilibrium configurations of lattice-based Metropolis Monte Carlo (MMC) simulations of phase separation. The key ingredient of the generalized Rouse model is a graph Laplacian that we compute from equilibrium MMC simulations. We compute two flavors of graph Laplacians, one based on a single-chain graph that accounts only for intra-chain contacts, and the other referred to as a collective graph that accounts for inter-chain interactions. Calculations based on the single-chain graph systematically overestimate the storage and loss moduli, whereas calculations based on the collective graph reproduce the measured moduli with greater fidelity. However, in the long time, low-frequency domain, a mixture of the two graphs proves to be most accurate. In line with the theory of Rouse and contrary to recent assertions, we find that a continuous distribution of relaxation times exists in condensates. The single crossover frequency between dominantly elastic vs dominantly viscous behaviors does not imply a single relaxation time. Instead, it is influenced by the totality of the relaxation modes. Hence, our analysis affirms that viscoelastic fluid-like condensates are best described as generalized Maxwell fluids. Finally, we show that the complex shear moduli can be used to solve an inverse problem to obtain the relaxation time spectra that underlie the dynamics within condensates. This is of practical importance given advancements in passive and active microrheology measurements of condensate viscoelasticity.

Assessing the Impact of Psychological Readiness on Performance and Symmetry in Functional Testing After ACL Reconstruction in Pediatric and Adolescent Patients.

Readiness for return to sports involves both physical and psychological aspects of recovery; however, the relationship between psychological and physical variables after anterior cruciate ligament (ACL) reconstruction (ACLR) is poorly understood. ACLR patients with a higher psychological readiness would demonstrate better functional testing results at 6 months. Cross-sectional study; Level of evidence, 3. Participants were evaluated at 6 months after ACLR with various patient-reported outcome metrics: Hospital for Special Surgery Pediatric Functional Activity Brief Scale, pediatric or adult International Knee Documentation Committee Questionnaire (IKDC), Patient-Reported Outcomes Measurement Information System (PROMIS) - Psychological Stress Experience and ACL - Return to Sport After Injury (ACL-RSI) scale. Functional testing included quadriceps, hamstrings, and gluteal strength testing; Y-balance test; single-leg single hop, crossover hop, and triple hop tests; and timed 6-m hop test. Pearson correlation coefficient and multivariable regression were used to determine associations between the limb symmetry index (LSI) on functional testing and patient-reported outcomes. Those with LSI deficits <20% (better performance) were compared with those with deficits >20% (worse performance). A total of 229 participants (89 male, 140 female) with a median age of 17 years (range, 10.3-30.6 years) were enrolled. IKDC had a moderate negative correlation with PROMIS - Psychological Stress Experience (r = -0.39; 95% CI = -0.49, -0.27; P < .001) and a moderate positive correlation with ACL-RSI (r = 0.55; 95% CI = 0.46, 0.64; P < .001). A total of 151 patients completed functional testing. ACL-RSI demonstrated a positive correlation with single-hop LSI (r = 0.21; 95% CI = 0.05, 0.35; P = .01) and timed 6-m hop (r = 0.28; 95% CI, 0.42; P = .001). When adjusting for sex, age, and graft type, patients who had <20% deficit on the single-hop test scored 16.6 points higher on ACL-RSI (P = .001), and those with <20% deficit on crossover hop testing scored a mean 13.9 points higher on ACL-RSI (P = .04). Higher psychological readiness for return to sport was associated with better performance and greater symmetry on hop testing 6 months after ACLR, suggesting a potential link between physical and psychological recovery.

Direct computations of viscoelastic moduli of biomolecular condensates.

In vitro facsimiles of biomolecular condensates are formed by different types of intrinsically disordered proteins including prion-like low complexity domains (PLCDs). PLCD condensates are viscoelastic materials defined by time-dependent, sequence-specific complex shear moduli. Here, we show that viscoelastic moduli can be computed directly using a generalization of the Rouse model and information regarding intra- and inter-chain contacts that is extracted from equilibrium configurations of lattice-based Metropolis Monte Carlo (MMC) simulations. The key ingredient of the generalized Rouse model is the Zimm matrix that we compute from equilibrium MMC simulations. We compute two flavors of Zimm matrices, one referred to as the single-chain model that accounts only for intra-chain contacts, and the other referred to as a collective model, that accounts for inter-chain interactions. The single-chain model systematically overestimates the storage and loss moduli, whereas the collective model reproduces the measured moduli with greater fidelity. However, in the long time, low-frequency domain, a mixture of the two models proves to be most accurate. In line with the theory of Rouse, we find that a continuous distribution of relaxation times exists in condensates. The single crossover frequency between dominantly elastic versus dominantly viscous behaviors is influenced by the totality of the relaxation modes. Hence, our analysis suggests that viscoelastic fluid-like condensates are best described as generalized Maxwell fluids. Finally, we show that the complex shear moduli can be used to solve an inverse problem to obtain distributions of relaxation times that underlie the dynamics within condensates.

Upgrading the Compressor Stage of the CAT250TJ Micro Gas Turbine Engine

AbstractDue to their simplicity and relative ease of manufacture, single-stage centrifugal and mixed flow micro gas turbine (MGT) engines are preferred in thrust-based remotely piloted aerial vehicles. A single-stage mixed-flow compressor upgrade for the 200 N CAT250TJ MGT engine is numerically evaluated and presented. An in-house developed mean line application and commercial CFD software is used for the design and performance evaluation of the proposed upgrade configurations. The CAT250TJ – Gen1 engine features a single-stage centrifugal compressor, annular combustor, and single stage axial turbine. Apart from an upgraded impeller, a new crossover diffuser configuration is introduced to replace the wedge-type, straight outlet diffuser configuration of the Gen1 engine. The new single vane crossover diffuser configuration provides a design point total-to-total efficiency and pressure ratio increase of 8.3% and 12.1%, respectively. A disadvantage of a single-vaned crossover diffuser compared to legacy diffusers is a narrower operating range. To alleviate this issue, various combinations of tandem and splitter vane crossover diffuser configurations are proposed. These provide an enhanced operating range, comparable with the operating range displayed by the Gen1 configuration. A turbine power matching analysis is additionally completed to ensure proper compressor integration. Gas turbine cycle software is used to evaluate the on-engine performance of the upgraded compressor configurations. It is shown that the new baseline, single vane crossover diffuser configuration provides a 10.74% increase in design point static thrust.

Abstract PO4-12-11: Patient (pt) time burden with IV vs subcutaneous (SC) administration of trastuzumab/pertuzumab (HP): A time and motion (T+M) substudy of a single arm phase II trial of adjuvant endocrine therapy plus HP for stage I HER2+ breast cancer

Abstract Background: The time required for in-clinic drug administration may have a substantial detrimental impact on breast cancer patients’ quality of life. SC drug administration, as opposed to IV, may reduce this time commitment. The purpose of this study was to estimate the relative difference in time and logistical burden between IV and SC administration of HP. Methods: We prospectively enrolled a pre-specified sub-cohort of pts participating on the ADEPT trial (NCT04569747) to this T+M substudy. The ADEPT trial is an ongoing single arm prospective phase II trial of adjuvant SC HP (for 1 yr) plus endocrine therapy for pts with hormone receptor-positive (HR+) and HER2+ stage I (pT1N0 or pT1N1mi) breast cancer. Pts eligible for ADEPT have completed primary breast surgery and are systemic therapy-naïve. Pts who received cycle 1 of loading SC HP at Dana-Farber Cancer Institute and tolerated SC drug administration were eligible and enrolled consecutively to the T+M substudy until the T+M accrual goal; accrual to the T+M substudy was mandatory for eligible pts. The T+M substudy was a single arm crossover design in which pts received 2 cycles of IV HP, followed by 2 cycles of maintenance dose SC HP. During those 4 treatment (tx) cycles, timepoints in the drug preparation and administration process were captured from the electronic medical record or flowsheets filled out by infusion nurses. The primary endpoint of the T+M substudy was total pt chair time in the infusion area (measured from start of first drug administration to end of final post-drug observation period). Exploratory endpoints included total drug preparation time and total pt tx experience time. A sample size of 22 pts was estimated to provide 86% power with 2-sided alpha 0.05 to detect a difference of 70 mins in the primary endpoint by tx arm (IV vs SC). For all endpoints, the average time across both tx cycles was calculated for each pt, and the overall mean across pts for each of IV and SC administration was computed (with standard deviation). Average times for IV vs SC administration were compared via paired Wilcoxon signed rank tests. Adjustments for multiple comparisons were not included. Results: 22 pts participated on the T+M substudy, and each had timepoints captured during 4 tx cycles (2 IV and 2 SC cycles), for a total of 88 patient-cycles analyzed overall. Pts had median age 58 yrs (range 42-83), and were 96% female, 82% White, 9% Black, 9% Asian; and 100% non-Hispanic. Comparison of time intervals for IV vs SC drug administration is shown in the Table. Mean total pt infusion chair time was 84.3 mins with IV HP, vs 22.5 mins with SC HP, for a difference of 61.8 mins shorter pt time commitment in favor of SC administration (p&amp;lt; 0.0001). The mean total drug preparation time was significantly decreased in favor of SC administration (by 78.2 mins; p&amp;lt; 0.0001); and the total pt tx experience time was significantly decreased in favor of SC administration (by 81.8 mins; p&amp;lt; 0.0001). Conclusions: This prospective T+M study demonstrated that pt infusion chair time is significantly decreased with SC as compared to IV administration of HP, shortening this pt time burden by approximately one hour. SC drug administration is an approach that may help to improve and shorten the pt breast cancer therapy experience and decrease pharmacy burden. Table. SD, standard deviation Citation Format: Adrienne Waks, Noah Graham, Emily Chen, Anna Mae Frey, Victoria Attaya, Ibrahim Abbass, Anita Fung, Jesse Sussell, Patricia Cortazar, Caroline Harvey, Kenneth Almeida, Denise Leth, Wendy Chen, Jose Leone, Susan Schumer, Nabihah Tayob, Sara Tolaney. Patient (pt) time burden with IV vs subcutaneous (SC) administration of trastuzumab/pertuzumab (HP): A time and motion (T+M) substudy of a single arm phase II trial of adjuvant endocrine therapy plus HP for stage I HER2+ breast cancer [abstract]. In: Proceedings of the 2023 San Antonio Breast Cancer Symposium; 2023 Dec 5-9; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2024;84(9 Suppl):Abstract nr PO4-12-11.

A RANDOMIZED CONTROLLED TRIAL ASSESSING A BRACE-FREE AND ACCELERATED WEIGHT-BEARING PROTOCOL AFTER SURGICAL REPAIR OF ACUTE PROXIMAL HAMSTRING TENDON AVULSIONS

INTRODUCTION &amp; AIMS Proximal hamstring tendon avulsion from the ischial tuberosity is a significant injury, with surgical repair advocated. After surgery, limited data exists regarding the optimal rehabilitation regime with extensive variation in published protocols. This study investigated patient outcomes following a traditionally conservative (CR) versus accelerated (AR) rehabilitation regimen after proximal hamstring tendon repair. METHODS This prospective randomized controlled trial (RCT) allocated 50 patients undergoing proximal hamstring tendon repair to either a braced, partial weight-bearing rehabilitation regime (CR=25) or an accelerated, unbraced regime, that permitted full weight-bearing as tolerated (AR=25). Patients were evaluated pre-operatively and at 3, 6 and 12 months post-surgery, via patient-reported outcome measures (PROMs) including the Lower Extremity Functional Scale (LEFS), Perth Hamstring Assessment Tool (PHAT) and 12-item Short Form Health Survey (SF-12). The single (SHD), triple (THD) and triple crossover (TCHD) hop tests were assessed at 6 and 12 months, as was peak isometric hamstring strength and peak isokinetic knee extensor and flexor torque. RESULTS All PROMs improved (p&amp;gt;0.05) and, while the AR group reported a significantly better Physical Component Score for the SF-12 at 3 months (p=0.022), as well as a lower severity of hamstring pain at 12 months (p=0.032), there were no other group differences. Peak isometric hamstrings strength and peak isokinetic quadriceps and hamstrings torque symmetry were all comparable between groups (p&amp;gt;0.05). While the AR group demonstrated significantly better (p=0.009) limb symmetry for the THD at 6 months, no other hop test differences were seen between groups. Three re-injuries have been observed (CR=2, AR=1). CONCLUSION After proximal hamstring repair surgery, post-operative outcomes following an accelerated rehabilitation regimen were largely comparable to a traditionally conservative

Thermodynamic crossovers in supercritical fluids

Can liquid-like and gas-like states be distinguished beyond the critical point, where the liquid-gas phase transition no longer exists and conventionally only a single supercritical fluid phase is defined? Recent experiments and simulations report strong evidence of dynamical crossovers above the critical temperature and pressure. Despite using different criteria, many existing theoretical explanations consider a single crossover line separating liquid-like and gas-like states in the supercritical fluid phase. We argue that such a single-line scenario is inconsistent with the supercritical behavior of the Ising model, which has two crossover lines due to its symmetry, violating the universality principle of critical phenomena. To reconcile the inconsistency, we define two thermodynamic crossover lines in supercritical fluids as boundaries of liquid-like, indistinguishable, and gas-like states. Near the critical point, the two crossover lines follow critical scalings with exponents of the Ising universality class, supported by calculations of theoretical models and analyses of experimental data from the standard database. The upper line agrees with crossovers independently estimated from the inelastic X-ray scattering data of supercritical argon, and from the small-angle neutron scattering data of supercritical carbon dioxide. The lower line is verified by the equation of states for the compressibility factor. This work provides a fundamental framework for understanding supercritical physics in general phase transitions.

An Efficient Binary Hybrid Equilibrium Algorithm for Binary Optimization Problems: Analysis, Validation, and Case Studies

Binary optimization problems belong to the NP-hard class because their solutions are hard to find in a known time. The traditional techniques could not be applied to tackle those problems because the computational cost required by them increases exponentially with increasing the dimensions of the optimization problems. Therefore, over the last few years, researchers have paid attention to the metaheuristic algorithms for tackling those problems in an acceptable time. But unfortunately, those algorithms still suffer from not being able to avert local minima, a lack of population diversity, and low convergence speed. As a result, this paper presents a new binary optimization technique based on integrating the equilibrium optimizer (EO) with a new local search operator, which effectively integrates the single crossover, uniform crossover, mutation operator, flipping operator, and swapping operator to improve its exploration and exploitation operators. In a more general sense, this local search operator is based on two folds: the first fold borrows the single-point crossover and uniform crossover to accelerate the convergence speed, in addition to avoiding falling into local minima using the mutation strategy; the second fold is based on applying two different mutation operators on the best-so-far solution in the hope of finding a better solution: the first operator is the flip mutation operator to flip a bit selected randomly from the given solution, and the second operator is the swap mutation operator to swap two unique positions selected randomly from the given solution. This variant is called a binary hybrid equilibrium optimizer (BHEO) and is applied to three common binary optimization problems: 0–1 knapsack, feature selection, and the Merkle–Hellman knapsack cryptosystem (MHKC) to investigate its effectiveness. The experimental findings of BHEO are compared with those of the classical algorithm and six other well-established evolutionary and swarm-based optimization algorithms. From those findings, it is concluded that BHEO is a strong alternative to tackle binary optimization problems. Quantatively, BHEO could reach an average fitness of 0.090737884 for the feature section problem and an average difference from the optimal profits for some used Knapsack problems of 2.482.

The effect of a tandem vane crossover diffuser configuration on the performance of a MGT mixed flow compressor

Due to the inherent geometric restrictions of a micro gas turbine (MGT) mixed flow compressor design, diffuser design has a major impact on compressor performance and operating range. Vaned diffusers are often preferred to vaneless diffusers in MGT compressor designs due to better efficiency and pressure recovery, albeit at the expense of operating range. A numeric investigation of the effect of a tandem vane crossover diffuser configuration on the performance and operating range of a MGT mixed flow compressor stage is presented. Three baseline test compressors, covering a wide range of design speeds, mass flow rates and meridional exit angles, are designed with a single vane crossover diffuser. For each of the three baseline compressors, the crossover diffuser design is modified to feature various tandem vane configurations. The tangential shift of the second vane row relative to the first row is evaluated. Additionally, relative first and second vane row lengths are evaluated. The performance (efficiency and pressure ratio) and operating range of the modified diffuser configurations are compared to the baseline configurations. It is shown that a 75% second vane row tangential shift configuration displays the best overall performance, regardless of the relative vane length. It is further shown that an appropriate vane length depends on the design requirement.

Transphyseal ACL reconstruction and tenodesis in skeletally immature patients demonstrates encouraging clinical scores, without growth disturbance, excessive laxity or re-injury

PurposePaediatric patients demonstrate high re-rupture rates after anterior cruciate ligament reconstruction (ACLR), with numerous surgical techniques proposed to deal with this challenging cohort. This study investigated the early clinical outcomes, complications, return to sport (RTS) and re-rupture rates up until 2-years post-surgery in paediatric patients presenting with open growth plates undergoing transphyseal ACLR that was combined with an extra-articular tenodesis (LET). MethodsBetween October 2017 and September 2020, 20 skeletally immature patients were consecutively recruited and underwent transphyseal ACLR and LET. Patient reported outcome measures (PROMs), KT-1000 laxity, knee range of motion (ROM), maximal isokinetic knee torque and a 3-hop battery were assessed at 6-, 12- and 24-months. Limb Symmetry Indices (LSIs), RTS rates, complications, re-ruptures and re-operations were reviewed. ResultsAll PROMs improved (p < 0.05). No change (p = 0.903) in laxity between limbs was seen, while 18 patients (90%) demonstrated normal (<3 mm) or near normal (3–5 mm) laxity differences. Peak knee flexion ROM improved over time (p = 0.028), while LSIs for knee extensor strength (p < 0.001), the single (p = 0.002) and triple crossover (p = 0.038) hop tests improved. At 24 months, 18 patients (90%) were participating in their pre-injury pivoting sport activities. No complications, growth disturbances, re-injuries or subsequent surgeries were observed. ConclusionsTransphyseal ACLR combined with LET, undertaken in skeletally immature paediatric patients, demonstrated high scoring PROMs, physical performance and RTS overall, without evidence of growth disturbance or excessive graft laxity. No re-injuries have been observed at this time with ongoing review required in this high-risk cohort.

Oreo Cookie Treatment Lowers LDL Cholesterol More Than High-Intensity Statin therapy in a Lean Mass Hyper-Responder on a Ketogenic Diet: A Curious Crossover Experiment.

Recent research has identified a unique population of 'Lean Mass Hyper-Responders' (LMHR) who exhibit increases in LDL cholesterol (LDL-C) in response to carbohydrate-restricted diets to levels ≥ 200 mg/dL, in association with HDL cholesterol ≥ 80 mg/dL and triglycerides ≤ 70 mg/dL. This triad of markers occurs primarily in lean metabolically healthy subjects, with the magnitude of increase in LDL-C inversely associated with body mass index. The lipid energy model has been proposed as one explanation for LMHR phenotype and posits that there is increased export and subsequent turnover of VLDL to LDL particles to meet systemic energy needs in the setting of hepatic glycogen depletion and low body fat. This single subject crossover experiment aimed to test the hypothesis that adding carbohydrates, in the form of Oreo cookies, to an LMHR subject on a ketogenic diet would reduce LDL-C levels by a similar, or greater, magnitude than high-intensity statin therapy. The study was designed as follows: after a 2-week run-in period on a standardized ketogenic diet, study arm 1 consisted of supplementation with 12 regular Oreo cookies, providing 100 g/d of additional carbohydrates for 16 days. Throughout this arm, ketosis was monitored and maintained at levels similar to the subject's standard ketogenic diet using supplemental exogenous d-β-hydroxybutyrate supplementation four times daily. Following the discontinuation of Oreo supplementation, the subject maintained a stable ketogenic diet for 3 months and documented a return to baseline weight and hypercholesterolemic status. During study arm 2, the subject received rosuvastatin 20 mg daily for 6 weeks. Lipid panels were drawn water-only fasted and weekly throughout the study. Baseline LDL-C was 384 mg/dL and reduced to 111 mg/dL (71% reduction) after Oreo supplementation. Following the washout period, LDL-C returned to 421 mg/dL, and was reduced to a nadir of 284 mg/dL with 20 mg rosuvastatin therapy (32.5% reduction). In conclusion, in this case study experiment, short-term Oreo supplementation lowered LDL-C more than 6 weeks of high-intensity statin therapy in an LMHR subject on a ketogenic diet. This dramatic metabolic demonstration, consistent with the lipid energy model, should provoke further research and not be seen as health advice.

CAN KINETICS AND KINEMATICS OF SINGLE LEG FORWARD AND CROSSOVER TRIPLE HOP TESTS DETERMINE RECREATIONAL MALE ATHLETES' RETURN TO SPORTS AFTER ACL RECONSTRUCTION?

Hop tests are used to determine return to sports after ACL reconstruction. They mostly measure distance and symmetry but do not assess kinematics and kinetics. Recently, biomechanical evaluations have been incorporated into these functional jump tests for the better assessment of return to sport. We assessed the sagittal plane range of motion (ROM) of the knee, the deviation axis of rotation (DAOR), and the vertical ground reaction force (vGRF) normalized to body weight in nine healthy participants during the single leg (SLH) and crossover hop tests (COHT).Participants' leg lengths were measured. Jumping distances were marked in the test area as being 4/5 of the leg length. Four sensors were placed on the thighs, the legs and the feet. These body parts were handled as a single rigid body. Eight 480 Hz cameras were used to capture the movements of these rigid bodies. vGRF at landing were measured using a force plate (Bertec, Inc, USA). The ROM of the knee joint and the DAOR were obtained from kinematic data.Participants' joint kinematics metrics were similar in within-subjects statistical tests for SLH and COHT. We therefore asked whether the repeated vGRF normalized to body weight will be similar in both legs during these jumps. Joint kinematics metrics however were different in between subjects indicating the existence of a personalized jumping strategy. These hop tests can be recorded at the beginning of the training season for each individual, which can establish a comparative evaluation database for prospective lower extremity injury recovery and return to sport after ACL injury.

Optimizing Production Schedules: Balancing Worker Cooperation and Learning Dynamics in Seru Systems

This paper aims to investigate the seru scheduling problem while considering the dual effects of worker cooperation and learning behavior to minimize the makespan and order processing time. Given the complexity of this research problem, an improved shuffled frog leaping algorithm based on a genetic algorithm is proposed. We design a double-layer encoding based on the problem, introduce a single point and uniform crossover operator, and select the crossover method in probability form to complete the evolution of the meme group. To avoid damaging grouping information, the individual encoding structure is transformed into unit form. Finally, numerical experiments were conducted using numerical examples of large and small sizes for verification. The experimental results demonstrate the feasibility of the proposed model and algorithm, as well as the necessity of considering worker dual behavior in the seru scheduling problem.

Time-Restricted Fasting Improves Liver Steatosis in Non-Alcoholic Fatty Liver Disease—A Single Blinded Crossover Trial

Background: Non-alcoholic fatty liver disease (NAFLD) is associated with visceral adiposity. We assessed the effectiveness of time-restricted fasting (TRF) for 16 h daily without calorie restrictions compared to standard care (SC; diet and lifestyle advice) in improving visceral adiposity and steatosis via controlled attenuation parameter (CAP). Methods: In a prospective single-blind randomized controlled trial, 32 participants with NAFLD were randomly assigned to TRF or SC for 12 weeks. The secondary endpoints were changes in liver stiffness, anthropometry, blood pressure, and other metabolic factors. Results: Twenty-eight participants completed the first arm of the study (TRF = 14, SC = 14), with 23 completing the crossover arm (TRF = 10, SC = 13). The baseline demographics were similar between the groups. Intermittent fasting caused a significant decrease in hepatic steatosis (p = 0.038), weight (p = 0.005), waist circumference (p = 0.001), and BMI (p = 0.005) compared to standard care. Intermittent fasting also resulted in additional within-group changes that were not seen in the standard care intervention. Conclusion: TRF offers superior improvements in patients with NAFLD, improving steatosis, weight, and waist circumference despite a lack of change in overall caloric intake. Time-restricted fasting should be considered as a primary weight loss intervention in the context of NAFLD. Trial registration: ACTRN12613000935730.

Recreational male athletes’ single leg forward hop and crossover triple hop test kinetics, kinematics, peak frequency and magnitude alterations

Objective: Single-leg forward and crossover triple hop tests are predictors of recovery and return-to-sports after lower extremity injury and treatment. The purpose of the study is to assess the kinematic and kinetic feasibility and repeatability of a motion analysis base for these hop tests in recreational male athletes. Methods: Normal ranges of single- and crossover triple hop tests kinetics, kinematics, peak frequencies and magnitude alterations in spectral distributions were evaluated in 11 recreational male athletes. Ground reaction forces were measured using a force plate. A motion capture system was used to record peak knee flexion-extension angles. Peak frequency and magnitude characteristics in spectral distributions were calculated from these measurements. Results: Ground reaction force normalized to body weight at landing was 2.9±0.1 (N/kg*10) in both legs during repetitions. Average flight over total time was 0.49±0.03 and 0.38±0.01 (s/s) for single and crossover triple hop tests, respectively. Flight, preparatory and total jump times were 0.301±0.020, 0.331±0.033 and 0.641±0.038 s for single, and 0.261±0.014, 0.453±0.024 and 0.701±0.046 s for crossover tests. Knee angle variations except for peak flexion angles were similar in both extremities. Main frequency spectral characteristic was about 2-4 Hz. First and second peak frequencies for the left and right leg were 3.19±0.17 and 7.16±0.17, and 3.15±0.16 and 7.18±0.19 Hz, respectively. Hop tests presented similar flight times during repetitions. This was however different during crossover jumps tests: flight times were shorter while preparatory times were longer. First characteristic frequency and magnitude were common in two tests. Conclusions: Peak flexion and extension angles and second characteristic frequency in single tests were specific to each participant. Hop tests are responsive to different performance metrics and are repeatable and feasible, so these tests can be used to create a comparative database for athletes.

Serial changes of the side-branch ostial area after single crossover stenting with kissing-balloon inflation

PurposeWe aimed to investigate the serial change of the side-branch ostial area (SBOA) depended on the wire-position before Kissing-balloon inflation (KBI) in the single-stent strategy for bifurcation lesions separately in the left main coronary artery (LMCA) and in non-LMCA.MethodsPatients who underwent a single-stent KBI for a bifurcation lesion and had OCT images at the timing of the rewiring, at the post-procedure, and at the 9-month follow-up were extracted from the 3D-OCT Bifurcation Registry, which is a multicenter-prospective registry of patients with a percutaneous coronary intervention for a bifurcation lesion under OCT guidance. The SBOA was measured by dedicated software, and the rewiring position at the side-branch ostium after crossover stenting was assessed by three-dimensional-optical coherence tomography (3D-OCT). The optimal rewiring was defined as link-free-type and distal rewiring. The relationship between the optimal rewiring and the serial change of the SBOA was investigated separately in LMCA and non-LMCA cases.ResultsWe examined 75 bifurcation lesions (LMCA, n = 35; non-LMCA, n = 40). The serial changes of the SBOA with the optimal rewiring were not significantly different regardless of LMCA and non-LMCA (LMCA:3.96 to 3.73 mm2, p = 0.38; non-LMCA:2.16 to 2.21 mm2, p = 0.98), whereas the serial changes of the SBOA with the sub-optimal rewiring were significantly reduced (LMCA:6.75 to 5.54 mm2, p = 0.013; non-LMCA:2.28 mm2 to 2.09 mm2, p = 0.024). There was no significant difference in clinical events between the optimal and sub-optimal rewiring group regardless of the LMCA and non-LMCA.ConclusionThe side-branch ostial area dilated with the optimal rewiring position in a bifurcation lesion treated with single crossover stenting and kissing-balloon inflation was preserved regardless of whether the bifurcation was in the LMCA or a non-LMCA.

Effectiveness of sleeper’s stretch on proprioception and range of motion in volleyball and basketball athletes with glenohumeral internal rotation deficit

Abstract Glenohumeral internal rotation deficit (GIRD) is a common impairment of neuromuscular origin that affects overhead athletes. The purpose of this study was to investigate the effect of 4-week sleeper stretch program on proprioception and range of motion (ROM) in athletes clinically diagnosed with GIRD. This study was a single group crossover study design (pre-post). The athletes underwent sleeper stretching program (5 sessions/week for 4 weeks) on the affected and non-affected sides. A total of 32 male athletes (15 basketball/17 volleyball were assessed for outcome measures of internal rotation (IR), external rotation (ER), total range of motion (TROM), and proprioception in ER, IR, and TROM (eyes open and eyes closed) at baseline and at the end of intervention. The affected side ROM significantly improved from 54.53±8.90° to 77.56±4.33° and 58.27±3.33° to 81.10±3.67° for volleyball and basketball athletes, respectively (IR ROM); from 114.56±5.88° to 95.77±6.66° and 119.44±4.67° to 104.23±5.05° for volleyball and basketball athletes, respectively (ER ROM); and from 169.90±5.78° to 173.11±5.88° and 177.28±5.88° to 186.89±3.79° for volleyball and basketball athletes, respectively (TROM), after the four-week sleeper stretch exercise. The ROM changes were non-significant on the non-affected side (P&gt;0.05). The ANOVA reading for individual sports was found to be significant: volleyball athlete IR: [P&lt;0.05, effect size (ES)=0.63], basketball athlete IR: [P&lt;0.05, ES=0.74], volleyball athlete ER [P&lt;0.05, ES=0.49), basketball athlete ER [P&lt;0.05, ES=0.38). However, there was no significant improvement in proprioception measured with both eyes open (EO) and eyes closed (EC) for the IR, ER, and TROM. This study establishes the important role of sleeper’s stretching protocol in improvement of internal rotation, external rotation, and total rotational motion of the affected side. These findings may have important clinical implications in the management of GIRD in volleyball and basketball athletes.