Simultaneous Determination Of Histamine Research Articles

A rapid determination of histamine and 3-methylhistamine in human plasma by high performance liquid chromatography

Histamine plays an important pathophysiological role in allergy, inflammation, gastric acid secretion, microcirculation and neurotransmission. 3-Methylhistamine is a prominent metabolite of histamine. Different methods for determination of histamine in biological fluids have been developed. In the present study, a simple, simultaneous determination of histamine and 3-methylhistamine by HPLC (precolumn derivatization with fluorescamine) was developed in human plasma using fluorescence detection with 1-methylhistamine as the internal standard. Linear regression analysis of the ratios of the concentrations of histamine and 3-methylhistamine (X) against peak height ratios (Y) yielded the following: y = 0.0073x − 0.0096 (R 2 = 0.990) and y = 0.0077x − 0.0111 (R 2 = 0.989). In conclusion, it was possible to detect histamine and 3-methylhistamine below 5 ng mL−1 in 1 mL plasma.

Simultaneous determination of histamine and histidine by liquid chromatography following intramolecular excimer-forming fluorescence derivatization with pyrene-labeling reagent.

A highly sensitive and selective fluorometric method for the determination of histamine and histidine has been developed. This method is based on an intramolecular excimer-forming fluorescence derivatization with a pyrene reagent followed by reversed-phase liquid chromatography. The analytes, containing two amino moieties in a molecule, were converted to the corresponding dipyrene-labeled derivatives by derivatization. The derivatives afforded intramolecular excimer fluorescence (440 - 540 nm), which can clearly be discriminated from the normal fluorescence (360 - 420 nm) emitted from reagent blanks. The detection limits (signal-to-noise ratio = 3) were femto mole levels.

A study on the vital reaction in wounded skin: Simultaneous determination of histamine and polyamines in injured rat skin by high-performance liquid chromatography

This paper describes a modified method of simultaneous quantitative determination of histamine and polyamines (putrescine, cadaverine, spermidine and spermine) in incised skin wounds of rats using high-performance liquid chromatography (HPLC) with a strong-cation exchange column, shim-pack ISC-05/S0504P, employing a linear gradient of 0.7–2.5 M sodium chloride. There was scarcely any change in the histamine content of the vital wounds throughout the one week experimental period, although content decreased slightly in 1-h-old wounds. On the other hand, concentrations of putrescine and spermidine increased suddenly 12 h after wounding, and spermine content increased slightly in 4-day-old wounds. There was no post-mortem change in the histamine, spermidine and spermine contents of vital wounds allowed to stand at 25°C up to 24 h after death. Based on these results, this HPLC method may be useful for the determination of wound age during the period of healing.

Simultaneous determination of histamine and spermidine by second-derivative synchronous fluorescence spectrometry

The method is based on formation of the fluorescent condensation products with o-phthaldialdehyde; 0.5–2000 ng ml −1 histamine and 3–700 ng ml −1 spermidine can be quantified, with relative standard deviations of 2–3%. Histamine/spermidine ratios of 2.5:1–1:30 can be handled. A selectivity study is reported.

Simultaneous determination of histamine and Nτ-methylhistamine with high-performance liquid chromatography using electrochemical detection

We have developed a liquid chromatographic method which uses electrochemical detection for the simultaneous quantitation of histamine and N τ-methylhistamine in rat brain. The amines are derivatized with the water-soluble Bolton-Hunter reagent (sulfo B-H). Perchloric acid extracts of rat brains are chromatographed on a strong cation-exchange resin. The eluate is evaporated and allowed to react with sulfo B-H at pH 9.8 at room temperature. The derivatization is complete after 30 s vortexing. The derivatives are purified using a cellulose-phosphate fibrous cation exchanger. They are quantified with an electrochemical detector at a potential of 0.56 V after preoxidizing the sample at 0.47 V. The derivatives of histamine, N τ-methylhistamine, and N α-methylhistamine are completely separated without interfering peaks. Since no N α-methylhistamine was detected in rat brain it was used as an internal standard. The detection limits are 0.1 pmol of histamine and 0.2 pmol of N τ-methylhistamine. The precision of this method is high, with within-run and between-run coefficients of variation of 2–7% and linearity of 0.999. Both histamine and N τ-methylhistamine peak heights increased significantly and selectively after treatment with pargyline. Because of the high sensitivity, accuracy, and precision, the histamine and N τ-methylhistamine contents of single nuclei of the rat hypothalamus can be routinely quantified.

P-172) - Simultaneous determination of histamine, its associate substances, chlorpheniramine and cimetidine by high-performance liquid chromatography

P-172) - Simultaneous determination of histamine, its associate substances, chlorpheniramine and cimetidine by high-performance liquid chromatography

Simultaneous determination of histamine and N τ-methylhistamine in human urine and rat brain by high-performance liquid chromatography with fluorescence detection

Simultaneous determination of histamine and N τ-methylhistamine in human urine and rat brain by high-performance liquid chromatography with fluorescence detection

Fluorimetric assay of histamine N-methyltransferase by high-performance liquid chromatography.

A sensitive method for the assay of histamine N-methyltransferase in rat tissues is described. Nτ-Methylhistamine formed from histamine under the optimum conditions for the enzyme reaction is extracted with chloroform, derivatized to a fluorescent product with the use of o-phthalaldehyde and 2-mercaptoethanol, and determined by high-performance liquid chromatography with fluorescence detection. This method allows the simultaneous determination of histamine and Nτ-methylhistamine. The limit of detection for Nτ-methylhistamine formed is 3.5 pmol per assay. The method is simple and is suitable for the routine assay of histamine N-methyltransferase in sample preparations obtained from tissues.

Simultaneous determination of histamine and N τ-methylhistamine in human plasma and urine by gas chromatography-mass spectrometry

A new and sensitive method is described for the determination of histamine and N τ-methylhistamine in human plasma and urine by gas chromatography-mass spectrometry. 15N 2-Labeled histamine and N τ-[methyl-d 3]methylhistamine were used as internal standards. Histamine and N τ-methylhistamine were converted to the derivatives N α-heptafluorobutyryl-N τ-ethoxycarbonylhistamine and N α-heptafluorobutyryl-N τ-methylhistamine, respectively. After these derivatives had been purified on a small column packed with CPG-10, the molecular ions were monitored during selected ion monitoring. Linear standard curves were obtained in the range of 0.5–10 ng/ml for both compounds. The reliability of the histamine analysis was demonstrated by using two different ion pairs, while a comparison with results from two different derivatizations on the same urine sample also established the specificity of the N τ-methylhistamine analysis. An increase of 1 ng of histamine in the plasma could be precisely determined by the present method. The histamine content of plasma from five normal subjects was determined as 0.83 ÷ 0.37 (S.D.) ng/ml and the N τ-methylhistamine content in most subjects was below the limits of this measurement. High excretion of histamine was noted in the urine collected in the early morning from a patient with nephritis.

Simple and Rapid Paper Chromatographic Method for the Simultaneous Determination of Histidine and Histamine in Fish Samples

A simple and rapid method for the simultaneous determination of histamine and histidine in fish samples is described. The method entails paper chromatography and is sensitive from 0.5 mug to at least 30 mug. The method is reproducible to within +/- 5% and compares favorably with a biological method for histamine determination.