Simplified Laboratory Procedure Research Articles

Clinical Evaluation of Denture Retention by Multi-suction Cup and Denture Adhesive

AIM: The aim of the study was to compare the retention of two modalities: Multi-suction cup denture, and denture adhesive and to evaluate the change of retention by different time intervals.
 PATIENTS AND METHODS: Twelve completely edentulous patients were selected. The patients received two dentures: One conventional denture, and the other with multi-suction cups. The retention was measured by a universal testing machine at insertion, 15 min, 30 min, 1 h, 2 h, and 4 h. All values were recorded in Newtons. Statistical analysis was carried out using two-way analysis of variance with post hoc Tukey’s test.
 RESULTS: Retention was higher in denture adhesive than multi-suction cup, and the change of retention was not statistically significant by time.
 CONCLUSION: Denture adhesive showed better retention clinically and simplified laboratory procedures than multi-suction denture.

005 Evaluation of Metal Loss through Simulated Rainfall-Induced Leaching of Salt-Based, Free-Choice Mineral Supplements: An Extension Service

Previous research identified preferential intake of Cu, Zn, and Mn-fortified supplements containing Zn and Mn hydroxychloride and basic Cu chloride vs. sulfate and organic forms of these metals among young beef calves. However, in the days following significant rainfall events, the preferential differences lessened. In addition, visual observation of the colored pooled water collecting under the raised mineral feeders suggested rainfall-induced leaching of trace minerals. Therefore, we designed a series of experiments to investigate rainfall-induced metal losses under laboratory conditions. From these efforts, a simplified laboratory procedure was established allowing benchtop evaluation of rainfall-induced leaching susceptibility from free-choice, salt-based mineral supplements. This procedure is now available as an extension service of the UF-IFAS, Range Cattle Research and Education Center. Briefly, the procedure is designed to simulate a 10.2 cm rainfall occurring over 3 separate events (3.4 cm on Monday, Wednesday, and Friday). The procedure requires a minimum of 4 kg of mineral supplement being tested, which is divided into 4, 1 kg subsamples. A single pre-leaching sample (250 g) is collected from each of the subsamples and the remaining 750 g of sample is placed into Buchner funnels (177 cm2) on 20 to 25 µ pore filter paper. Deionized water (pH adjusted to 5.6) is poured over the mineral and the leachate collected into volumetric flasks. Along with the original pre-leaching sample, the remaining leached mineral is dried, weighed and analyzed for Cu, Zn, and Mn concentration using inductively coupled plasma spectroscopy techniques. Metal loss is estimated by calculating the difference between the total metal in the initial pre-leached sample less the metal remaining in the leached sample. Results are presented as percent metal loss ± SD of the 4 replications. The service is offered for $350 per sample with a turnaround time of approximately 14 d. In addition to leaching loss of trace metals, the analysis also provides important comparison between labeled nutrient specifications and actual chemical analysis. Results provide both manufacturers and producers with an understanding of the potential for essential nutrient losses due to rainfall interactions with free-choice, salt-based mineral and assist in evaluating the economic advantages of increased frequency of supplementation, use of weathering agents, and investment in rainfall-protected mineral feeders.

Fabricating a Ceramic-Pressed-to-Metal Restoration with Computer-Aided Design, Computer-Aided Manufacturing and Selective Laser Sintering: A Case Report

Even though a conventional metal ceramic restoration is widely in use, its laboratory procedure is still technique-sensitive, complex, and time-consuming. A ceramic-pressed-to-metal restoration (PTM) can be a reliable alternative. However, simplified laboratory procedure for a PTM is still necessary. The article is to propose a technique that reduces time and effort to fabricate a PTM with the aid of computer-aided design, computer-aided manufacturing and selective laser sintering technologies.

A Simplified Technique for Fabrication and Characterization of a Custom Ocular Prosthesis using Light-cured Stains

ABSTRACT An ocular prosthesis which gives attention to the accurate duplication of natural color, contour, size and ocular orientation will provide realism and symmetry for patients who need it. This article describes a simplified technique of converting a stock eye shell into a well-fitting custom ocular prosthesis that requires minimal clinical procedures and simplified laboratory procedures. The color matching of the sclera of the prosthesis was performed using light-cured resin-based stains that are simple to use and give a more predictable and lifelike appearance when compared to the conventional acrylic ‘paint on’ technique. How to cite this article Daswani SR, Aras MA, Chitre V, Mysore A. A Simplified Technique for Fabrication and Characterization of a Custom Ocular Prosthesis using Light- cured Stains. Int J Prosthodont Restor Dent 2015;5(1):17-20.

A modified technique and simplified laboratory procedure for Ocular Prosthesis Fabrication

PurposeIn many cases dentist tries to satisfy the patient with stock prosthesis that comes in standard sizes, shapes and colors. However, most of the patients complain of discomfort even after appropriate adjustment because of insufficient adaptation to underlying soft tissues. The rehabilitation of a patient with loss of eye as a result of congenital defect, pathology or accidental trauma is a challenge in terms of re-establishment of the esthetics of the patient. The purpose of this technical procedure is to provide a well fitting prosthesis to the patient, reducing the number of laboratory steps and appointments for fabrication of ocular prosthesis. MethodA suitable stock eye shell was modified to fit the eye socket, followed by relining with low fusing compound and making a final impression with polyvinyl siloxane impression material. The impression was poured in die stone, flasking procedure was accomplished. After dewaxing, molten wax was flown in the lower half of the flask followed by repositioning of the upper half. The flask was reopened, the stock eye shell along with the hardened wax on tissue surface was tried in the patient's eye. Finally processing was done. ConclusionThis technique describes an impression procedure, followed by a wax pattern try-in in the same appointment and also the detailed laboratory procedure for fabrication of the ocular prosthesis, taking into consideration positioning of iris as a result enhancing the esthetic appearance of the patient.

New concepts in industrial wastewater management

The terms: "pollution prevention", "waste minimization", "source segregation" and "in-plant control", have been increasingly mentioned over the past decade in conferences and projects related to industrial wastewater management. While end-of-pipe treatment of industrial wastes was a popular management approach only 20 years ago, advanced treatment/management concepts are more common today. An in-plant control program including waste segregation and process-specific pretreatments has been proposed for a complex indus- trial park, in place of a traditional central treatment of the combined park's wastewater. A protocol that enables selection of waste streams amenable to biological treat- ment and identification of problematic streams requiring pretreatment is presented and demonstrated in this paper. It includes simplified laboratory procedures for chemical and toxicological characterization of source streams and for screening their removability potential by biodegradation, volatilization, and carbon adsorption. The management approach In any industrial plant the production of by-products and waste materials (either in the forms of liquid, solid or gas) is an increasing environmental and economical problem. Zero discharge is an optimal goal that cannot always be achieved. However, new management concepts such as source segregation and in-plant control may reduce the waste volume and improve its quality for further treatment and disposal. Thus the overall cost of the product (including production costs and treatment/ disposal expenses) may be reduced and environmental nuisances and hazards can be minimized. These concepts are gaining popularity over the traditional end-of-pipe treatment approach, due to their economical benefits gained by the industries themselves. Authorities are involved in this process in order to gain environmental benefits with no contradiction to the industries interests. Actual measures that can be implemented in an advanced in-plant management approach are detailed in Table 1.

Development of a Pretreatment Program to Improve Biological Treatability of High Strength and Toxic Industrial Wastewater

A biological treatment process has been suggested as the main treatment stage for a high (organic) strength industrial wastewater stream, discharged by several chemical industries within a large industrial park. Treatability studies have indicated that the wastes contain a fraction of toxic and non-biodegradable organic matter, which limits the implementation of a conventional biological treatment process for the combined wastewater stream. Therefore, an in-plant control program including waste segregation and process-specific pretreatments is proposed. A protocol that enables selection of waste streams amenable to biological treatment and identification of problematic streams requiring pretreatment is presented and demonstrated. It includes simplified laboratory procedures used for chemical and toxicological characterization of source streams originating in various processes. The results can be used for the development of a pretreatment program for problematic waste streams, based upon local small-scale solutions.

A simplified laboratory procedure to select an appropriate antibiotic for treatment of refractory periodontitis.

THE FEASIBILITY OF USING A simplified laboratory procedure to select an appropriate antibiotic for treatment of refractory periodontitis was investigated. Six different antibiotics were added to commercially available CDC-agar plates. Plaque samples from periodontal pockets were inoculated onto the plates. The percentage kill was determined by comparing colony counts on the antibiotic-containing medium with those on the control plates. The three most effective antibiotics were ranked by visual inspection as well as by colony count. It was determined that visual inspection may be used to determine an effective antibiotic.

Icosahedral carboranes. XVII. Simplified preparation of o-carborane

Abstract : A new synthesis of 1,2-dicarbaclosododecaborane is described which involves the reaction of acetylene with B10H12L2 compounds in the solid state. This simplified laboratory procedure provides pure o-carborane in one step as a sublimate from the reaction mixture. (Author)

In vitro platelet adhesiveness. Methods of study and clinical significance.

Wright demonstrated in 1941 that platelets adhere to glass (102). Her approach was revived by Hellem’s work in 1960 (40), which led to the discovery of the effect of ADP on platelets. Since then, there has been considerable interest in developing simplified laboratory procedures to measure platelet adhesiveness. It is usually assessed by methods utilizing the tendency of platelets to adhere to glass. Platelet counts are performed before and after blood has been exposed to a glass surface for variable periods of time. Platelet adhesiveness is expressed as the number of adhered platelets or,more commonly, as the percentage of platelets which adhere or stick to the beads.

High‐yield preparation of methyl stearolate

AbstractA simplified laboratory procedure for preparing methyl stearolate consists of three steps—bromination, dehydrobromination, and purification. A variety of starting materials was investigated, including oleic acid, olive fatty acids, and triglycerides. Brominations of both fatty acids and triglycerides were conducted in diethyl ether. Dehydrobrominations were carried out in boiling 30% KOH‐ethylene glycol solutions or in 30% KOH‐water solutions under pressure. Saponification of the triglycerides also occurred at this step. After conversion to methyl esters and distillation, the product from olive oil analyzed 79% methyl stearolate. Purification was accomplished by either argentation or acetonitrile‐hexane countercurrent distribution and yielded methyl stearolate of +99% purity. Over‐all recoveries, based upon the amount of oleic acid present in the initial oil, averaged 80%. In addition to the laboratory procedure, possible production operations are outlined.

Antihistaminics: The N-Beta-Dimethylaminoethyl Derivatives of Carbazole and Diphenylamine

Although β-dimethylaminoethyl chloride hydrochloride is a very important intermediate which has been especially useful in the synthesis of various antihistaminic agents, publications mentioning its use often neglect to refer to a convenient method of preparation. A simplified laboratory procedure for the preparation of this intermediate is reported and a description is given of its use in the synthesis of two antihistamine-type compounds derived from carbazole and diphenylamine.