Simple Spectrophotometric Method Research Articles

Development of an affordable light emitting diode spectrophotometer paired with a Python program for calibration and linearity testing and the measurement of uranium(VI).

Uranium (U) is a radiologically and chemically toxic element that occurs naturally in water, soil, and rock at generally low levels. However, anthropogenic uranium can also leach into groundwater sources due to mining, ore refining, and improper nuclear waste management. Over the last few decades, various methods for measuring uranium have emerged; however, most of these techniques require skilled scientists to run samples on expensive instrumentation for detection or require the pretreatment of samples in complex procedures. In this work, a Schiff base ligand (P1) is used to develop a simple spectrophotometric method for measuring the concentration of uranium (VI) with an accurate and affordable light-emitting diode (LED) spectrophotometer. A test for a higher-order polynomial relationship was used to objectively determine the calibration data's linearity. This test was done with a Python program on a Raspberry Pi computer that captured the spectrophotometer's calibration and sample measurement data.

Spectrophotometric and Chromatographic Determination of Alkaloids and Nicotine Contents in Lebanese Tobacco Leaves

Nicotiana Tabacum; the annual herb; known as Tobacco from the Solanaceae family was known for its alkaloids and especially nicotine (NCT) content; smoking products, insecticides, anthelminthic activity and clinically proven therapeutics are examples of its uses. Herein two different methods were applied in an attempt to quantify the total alkaloids and NCT content in Nicotiana tabacum cultivated in Lebanon. Total alkaloids were investigated through the formation of a complex with bromocresol green under a simple spectrophotometric method. Whilst HPLC-DAD was the choice for the quantitation of NCT levels. The column was Lichrospher select B (5 µm, 250x4 mm), the temperature was set at 29 ⁰C and the wavelength at 260 nm. The mobile phase consisted of 2 M O-phosphoric acid and methanol (60:40, v/v) using isocratic elution at 1 mL/min. A linear relationship was proved under both instruments. The extraction yield of alkaloid totum ranges between 2.1 ± 0.25 and 6.8% ± 0.58 and alkaloids contents range from 12.14 ± 2.01 to 53.12 ± 4.54 mg of AE/g of extract for Ghandouriyeh and Danniye samples respectively. On the other side among the different areas which cultivated Tobacco in Lebanon, Danniye was found to have the highest NCT concentration of dry weight (2.64%) while Al-Hissa possesses the lowest content (0.75%). Even if the results were generally similar to other countries, the study showed a difference in values from one region to another.

Spectrophotometric Determination of Selenium in Food Items

Selenium (Se) is an essential element that is obtained from food. To increase the level of selenium in the body, we should consume selenium-rich foods in our diet. The objective of this study was to develop a simple spectrophotometric method for the quantitative analysis of selenium present in food. This study included six food items such as broccoli, cabbage, red cabbage, fenugreek, garlic, and ginger. The new method is based on the yellow colour complex formed by the reaction between 2% potassium iodide and 0.2% pyridine in acidic medium with the selenium. Absorbance was measured at 400 nm against the reagent blank. The results of the above proposed method were compared with the reference method of using potassium iodide and starch as reagents. The selenium content in the examined food samples was determined from the regression equation of the calibration curve y= 0.0799x+0.3477 respectively. All the analyses were performed in triplicate, and the results are expressed as mean ± standard deviation. The highest mean selenium value was obtained in ginger and garlic 18.70 & 18.32 µg/g, respectively among all the studied samples, while the lowest value was in fenugreek seeds 7.38 µg/g. The proposed method is simple, rapid, sensitive, and obeys Beer–Lambert’s law. The proposed method was successfully applied to determine food items. Selenium has been quantitatively analysed for its potential to protect the body from many degenerative diseases. Selenium-rich food, as studied here, should be used as a supplement in the human diet.

Determination of the antioxidant content in selected medicinal plants of Kalyan and Kolhapur

The aim of this research is to qualitatively screen medicinal plants for the presence of various phytochemicals and to develop a simple spectrophotometric method for the quantitative analysis of flavonoids, phenolic content Ascorbic acid in Tulsi leaves, Tulsi stems, ajwain leaves, clove leaves, ginger rhizome and fenugreek seeds. This study provides an overview of flavonoids, phenolic content, and ascorbic acid in medicinal plants. The contents were determined by the proposed spectrophotometric method, and the obtained results were compared with the Reference method. Qualitative analysis of medicinal plants was performed, and it was observed that alkaloids, Flavonoids, tannins, phenolic compounds, proteins, oils, and fats were present in all plant extracts. TFC, TPC, and AA were determined by using a UV-Vis spectrophotometer. All analyses were performed in triplicate, and the results were expressed as mean ± standard deviation. Tulsi and Ajwain are found to be rich in flavonoids, whereas ginger and fenugreek are rich in phenols. Ascorbic acid is an essential antioxidant present in all medicinal plants. Fenugreek is rich in all antioxidants. The proposed method is simple, rapid, sensitive, and obeys Beer-Lambert’s Law.

Spectrophotometric Determination and Biological Activity Study of Zn(II) using a Newly Synthesized Azo Reagent Derivative from 4,5-bis(4-methoxyphenyl) Imidazole

A rapid and simple spectrophotometric method was submitted for Zn(II) determination using (E)-2-(4,5-bis(4-methoxyphenyl)-1H-imidazole-4-yl) diazenyl) benzoic acid as an analytical reagent. The suggested method is based on the chelation reaction between Zn(II) and the bidentate chelation ligand with a mole ratio of 1:2 (metal:ligand) with octahedral geometry. The maximum absorption of the formed violet complex was 560 nm, the linearity was in the concentration range of 0.5-15.0 µg mL-1. The relative standard deviation (n =10) was 0.149%. The interfering effect of diverse cations and anions was tested. To clarify the biological efficacy of the synthesized Zn(II) complex, an in vitro biological activity study was carried out against four kinds of bacterial strains, and the results showed promising inhibition activity.

A simple spectrophotometric method for chloroform quantification and its application in chloroform degradation from laboratory wastewater using polyphenolic co-activators

A simple spectrophotometric method for measuring high concentration of chloroform in water was developed based on the Reimer-Tiemann reaction. The reaction between chloroform and resorcinol under alkaline conditions has the limit of detection (LOD) and limit of quantification (LOQ) values at 1.28 mg/mL and 3.88 mg/mL, respectively. The experimental and response surface methodology (RSM) proposed the optimal conditions for the removal of chloroform (RoC) using tannic acid as a polyphenolic co-activator and spectrophotometric method for chloroform detection. The acceptable dechlorination process was suggested as persulfate (0.1–0.11 M), NaOH (0.6–0.8 M), and tannic acid (1.8 g/L) to obtain the yield of RoC from 76.14 to 77.90%. In situ experiments were then designed for RoC from the laboratory’s aqueous and organic solvent waste. After being treated with the system mentioned above, the yields of RoC were around 70%. In addition, a critical aspect of the innovation process was replacing pure tannic acid with other natural agents, which are more economically efficient and eco-safety. The EtOH extract from Elaeocarpus hygrophilus leaves (EEL) was suggested as a natural source of polyphenols, which could co-activate persulfate, with the yield of RoC rising to 63.29%. The findings of this study provide a novel strategy for chloroform degradation from laboratory wastewater using polyphenols.

DEVELOPMENT OF AN APPROACH TO DETERMINING ENZYMATIC ACTIVITY OF RIBONUCLEOSIDE HYDROLASE C USING HYDROPHILIC INTERACTION LIQUID CHROMATOGRAPHY

Ribonucleoside hydrolase C (RihC, EC 3.2.2.1-3.2.2.3, 3.2.2.7, 3.2.2.8) belongs to the family of ribonucleoside hydrolases that catalyze the cleavage of both purine and pyrimidine ribonucleosides to nitrogenous bases and ribose. Its most efficient reaction is the cleavage of uridine with the highest reaction rate. The reaction cannot be detected by a simple spectrophotometric method because of the same absorption maximum for the substrate and reaction product or requires time- and labor-consuming sample preparation for ribose. Reversed-phase HPLC is currently used to register enzymatic activity, where the time of one chromatographic run takes about 10 min. Since a large number of analyses are required to measure the kinetics of an enzymatic reaction, the total time is significant. In this work, we obtained new recombinant RihC from Limosilactobacillus reuteri by gene cloning and expression in E.coli cells. We proposed a new approach for determining the enzymatic activity of the new RihC using hydrophilic interaction liquid chromatography (HILIC). The novel column was developed for this procedure providing the determination of uracil and uridine with high efficiency and retention times of 0.9 and 1.7 min, respectively. Kinetic parameters for RihC uridine cleavage were determined. The proposed approach provided significant rapidity for measurement of the enzyme kinetics being 5 times faster as compared to reversed-phase HPLC.

Spectrophotometric Determination of Amoxicillin Using New Organic Reagent <i>via</i> Different Analytical Methods

New and simple spectrophotometric method was applied for amoxicillin determination by oxidative coupling with an organic reagent 1-(4-aminophenyl)-3-(5-(4-nitrophenyl)-furan-2-yl)-yl)-prop-2-en-1-one (H) to form an orange colored dye with λmax of 490 nm. The molecular structure of the new compound H was characterized using spectral analysis including 1H-NMR, FTIR, Mass spectroscopy, and UV-visible. The concentration range of oxidative coupling obeyed Beer's law was 2–50 μg/mL, the correlation coefficient was 0.9995, molar absorptivity was 0.63 × 104 L/mol cm, and the detection limit was 0.189 μg/mL. The concentration range of flow injection obeyed Beer's law was 1–150 μg/mL, the correlation coefficient was 0.9994, molar absorptivity was 0.295 × 104 L/mol cm, and the detection limit was 0.407 μg/mL. The proposed method was successfully applied in pharmaceutical formulation for amoxicillin determination. The results showed that amoxicillin could be reacted with a new compound H in the alkaline medium in the presence of oxidative agent NaIO4 and automated by flow injection analysis. The proposed methods have the advantage of simple, fast, very sensitive, good precision and accuracy. The suggested technique was effectively used to estimate amoxicillin in both its pure form and pharmaceutical formulations.

Validation of a simple spectrophotometric method for the rapid determination of salicylates in plasma

IntroductionThe determination of salicylate concentrations constitutes a critical aspect of medical diagnostics, particularly in emergency settings. High-performance liquid chromatography (HPLC) and spectrophotometry are efficient methods commonly utilized for this purpose. In emergency laboratories with limited resources, the validation of a cost-effective and reliable spectrophotometric method for salicylates in plasma becomes imperative. The present study aims to validate such a method, ensuring its applicability in toxicological emergencies within resource-constrained laboratories. Materials and methodsThe proposed spectrophotometric analysis relies on detecting salicylic ions amidst the presence of ferric salts, resulting in the formation of a distinct purple chelate complex. To ascertain the method's credibility, the validation guidelines established by the European Medicines Agency (EMA) were employed as a benchmark. A comprehensive validation process was conducted over a three-day period, with three levels of validation standards being considered. ResultsFollowing the EMA protocol, the spectrophotometric method demonstrated commendable fidelity, accuracy, and linearity over a concentration range of 50 to 500 mg/L. The limit of detection and quantification was found to be 10 and 50 mg/L, respectively, and the correlation coefficient was determined to be R2 = 0.998. However, it is essential to acknowledge that interference with phenothiazines occurred at concentrations ranging from 50 to 100 mg/L. Despite this, the method's average sensitivity remains viable for practical use in cases of poisoning. The accuracy per concentration level proved satisfactory, with relative biases remaining below 15%, and the confidence intervals of mean recovery closely approximating the desired target value of 100%. ConclusionIn conclusion, the presented spectrophotometric method stands out as an economical, straightforward, and user-friendly approach, ideally suited for toxicological emergencies when resources are limited. The method delivers satisfying results, establishing its practical utility in critical medical scenarios. This validated method holds immense promise for emergency laboratories facing resource constraints.

Assessment of Metformin Stability and Its Removal from Water by Pumice-Based Zeolite

Metformin (MET) is an emerging contaminant often found in aquatic environments due to incompletely metabolized after consumption in the human body, where some of its doses are excreted in unchanged form through urine or faeces. The research aims are to study MET stability in water and to evaluate the potential of pumice-based zeolite for treating MET contaminants. Zeolites were prepared from pumice via the hydrothermal treatment and characterized using Fourier Transform Infrared (FTIR) and X-Ray Diffraction (XRD). A simple spectrophotometric method was proposed for determining the stability of MET in aqueous solution with several different conditions. The result showed that the metformin solution was unstable under strong acid conditions (pH 2), marked by the loss of the maximum absorption peak in the 220-250 nm range. The stable metformin conditions in this study were obtained at the pH of solutions ranging from 6 to 10 and temperatures between 14 oC to 40 oC. Furthermore, the stability of MET can be maintained for up to 3 days of exposure time. Based on the adsorption result, zeolite uptake capacity (196 µg/g) was higher than pumice (87 µg/g) to reduce metformin concentration. In addition, the synthesized zeolite needs further modification to increase its adsorption performance.

Development of a method for the quantitative determination of glibenclamide in tablets

Aim. To develop and validate a spectrophotometric technique for quantitative determination of glibenclamide in tablets by reaction with 2,3-dichloro-1,4-naphthoquinone. Materials and methods. In the study, the substance glibenclamide of pharmacopoeial purity was used, tablets – “Maninil” 5 mg and “Glibenclamid-Zdorovye” 5 mg, 2,3-dichloro-1,4-naphthoquinone of the “CFA” qualification was chosen as a reagent, as a solvent – dimethylformamide (DMF) “CFA”. The following analytical equipment was used for the research: spectrophotometer “Specord-200” (Analytic Jena AG, Germany), water bath “MEMMERT WNB7”, laboratory electronic scales RADWAG XA 210. 4Y, measuring laboratory vessels of class A. Results. A new, simple spectrophotometric method of quantitative determination of glibenclamide in tablets by reaction with 2,3-dichloro-1,4-naphthoquinone in DMF medium was developed. The absorption maximum was at 489–491 nm. The value of the detection limit is 10.9 μg/ml, which indicates sufficient sensitivity of the reaction. Subordination to the basic law of light absorption is within the limits of concentrations of 13.7–27.4 mg/100 ml. In the process of developing the methodology, the following validation characteristics were determined: specificity, linearity, precision, correctness, and robustness. Conclusions. The methodology for the quantitative determination of glibenclamide was developed and validated according to the requirements of the State Pharmacopoeia of Ukraine. It has been proven that the method is simple, accessible, and validated for such characteristics as linearity, convergence and robustness and can be used for application in laboratories for quality control of medicinal products.

Spectrophotometric determination of L-α-glycerylphosphorylcholine in pharmaceutical formulations and industrial equipment cleaning rinse water with the WAKO Phospholipids C assay kit

A simple spectrophotometric method for the determination of L-α-glycerylphosphorylcholine in pharmaceutical formulations and industrial equipment cleaning rinse water using the enzyme glycerophosphocholine phosphodiesterase and the WAKO Phospholipids C assay kit was proposed. The method is based on the enzymatic hydrolysis of α-GPC to choline by glycerophosphocholine phosphodiesterase, the reaction of choline with the components of the assay kit, and the colourimetric determination of the formed product. The calibration graph is linear in the range from 1 to 40 mg/l of α-GPC, the molar attenuation coefficient is 1,110 m2/mol, the limit of detection is 1 mg/l, the limit of quantification is 3.3 mg/l, the method is selective with respect to the common excipients, shows a good accuracy (the relative uncertainty does not exceed 7%) and precision (the relative standard deviation does not exceed 5.5%), does not require lengthy sample preparation and sophisticated laboratory equipment and is suitable for the routine analysis of pharmaceutical formulations and industrial equipment cleaning rinse water.

Developing the Ascorbic Acid Test: A Candidate Standard Tool for Characterizing the Intrinsic Reactivity of Metallic Iron for Water Remediation

Granular metallic iron (gFe0) materials have been widely used for eliminating a wide range of pollutants from aqueous solutions over the past three decades. However, the intrinsic reactivity of gFe0 is rarely evaluated and existing methods for such evaluations have not been standardized. The aim of the present study was to develop a simple spectrophotometric method to characterize the intrinsic reactivity of gFe0 based on the extent of iron dissolution in an ascorbic acid (AA—0.002 M or 2 mM) solution. A modification of the ethylenediaminetetraacetic acid method (EDTA method) is suggested for this purpose. Being an excellent chelating agent for FeII and a reducing agent for FeIII, AA sustains the oxidative dissolution of Fe0 and the reductive dissolution of FeIII oxides from gFe0 specimens. In other words, Fe0 dissolution to FeII ions is promoted while the further oxidation to FeIII ions is blocked. Thus, unlike the EDTA method that promotes Fe0 oxidation to FeIII ions, the AA method promotes only the formation of FeII species, despite the presence of dissolved O2. The AA test is more accurate than the EDTA test and is considerably less expensive. Eight selected gFe0 specimens (ZVI1 through ZVI8) with established diversity in intrinsic reactivity were tested in parallel batch experiments (for 6 days) and three of these specimens (ZVI1, ZVI3, ZVI5) were further tested for iron leaching in column experiments (for 150 days). Results confirmed the better suitability (e.g., accuracy in assessing Fe0 dissolution) of the AA test relative to the EDTA test as a powerful screening tool to select materials for various field applications. Thus, the AA test should be routinely used to characterize and rationalize the selection of gFe0 in individual studies.

Development of a New Analytical Method for Estimation of Trifluoperazine-HCl in Pharmaceutical Formulation

The estimation of trifluoperazine hydrochloride in pure and dosage forms has been developed using a simple spectrophotometric method. The method is based on using an oxidation-reduction reaction with 4,7diphenyl 1,10-phenanthroline in the presence of Ferric)III( ion solution to produce a red-colored product complex of Fe(II )- 4,7diphenyl 1,10phenanthroline with maximum absorption at 533nm. The molar absorptivity is 0.44x104 L. mole -1.cm-1, Beer's law is followed over a concentration range of 5-100 μg. ml-1.Various parameters that influence the development and stability of the color product were thoroughly researched and optimized, and a reaction pathway proposal was offered. The proposed method was successfully used to determine trifluoperazine hydrochloride in tablet form.

Copper-Guanosine Nanorods (Cu-Guo NRs) as a Laccase Mimicking Nanozyme for Colorimetric Detection of Rutin

Inspired by laccase activity, herein, Cu-guanosine nanorods (Cu-Guo NRs) have been synthesized for the first time through a simple procedure. The activity of the Cu-Guo NR as the laccase mimicking nanozyme has been examined in the colorimetric sensing of rutin (Rtn) by a novel and simple spectrophotometric method. The distinct changes in the absorbance signal intensity of Rtn and a distinguished red shift under the optimum condition based on pH and ionic strength values confirmed the formation of the oxidized form of Rtn (o-quinone) via laccase-like nanozyme activity of Cu-Guo NRs. A vivid and concentration-dependent color variation from green to dark yellow led to the visual detection of Rtn in a broad concentration range from 770 nM to 54.46 µM with a limit of detection (LOD) of 114 nM. The proposed methodology was successfully applied for the fast tracing of Rtn in the presence of certain common interfering species and various complex samples such as propolis dry extract, human biofluids, and dietary supplement tablets, with satisfactory precision. The sensitivity and selectivity of the developed sensor, which are bonuses in addition to rapid, on-site, cost-effective, and naked-eye determination of Rtn, hold great promise to provide technical support for routine analysis in the real world.

Spectrophotometric method for Determination of oxymetazoline, HCl in a Pharmaceutical formulation using 2,4 - dinitrophenylhydrazine

Rapid, sensitive and simple spectrophotometric method for the determination of oxymetazoline hydrochloride (OXMZ) is developed. The method is based on an oxidative coupling reaction with 2,4-dinitrophenylhydrazine (2,4-DNPH) in a basic medium (pH 11.2) in the presence of potassium periodate to produce a green colour, soluble in water , stable product and absorbs at 607 nm. Beer's law was in the linear range 2.5-30 μg/ml of OXMZ, the molar absorptivity , Sandellʼs sensitivity index and detection limit were 7242 liter. mol-1.cm-1, 0.0409 μg.cm-2 and 0.1877 μg/ml respectively. The RSD value was 1.24- 1.39% depending on the concentration. This method was applied successfully to the determination of oxymetazoline in pharmaceutical preparation nazordin drops with recovery of not less than 99.87 % .

A Histogram-Based Technique for Simultaneous Colorimetric Determination of Malachite Green and Brilliant Green Using Triton X-100 Micelle.

Malachite green (MG) and brilliant green (BG) are two synthetic triphenylmethane dyes with applications in the textile and aquaculture industries. They are considered to be environmental contaminants due to their carcinogenic and mutagenic properties. Both dyes have the same bluish-green color in aqueous solutions. The aim of this study is to develop a colorimetric analysis as a very simple and cost-effective method to determine the residues of MG and BG simultaneously in aqueous industrial samples. This method is based on the alterations in red, blue, green (RGB) color histograms of the dyes in the presence and absence of Triton X-100 micelle. The images of the samples were taken by a digital camera and converted to the RGB color system using MATLAB software. Partial least-squares regression as a powerful chemometrics tool was used for multivariate calibrations and quantitative measurements. The performance of the proposed method was compared with a simple spectrophotometric method as a reference. Relative errors of prediction for colorimetric and spectrophotometric analysis, respectively, in micellar media were 6.56 and 4.61% for MG and 6.38 and 5.24% for BG. The shortest linear ranges for colorimetric and spectrophotometric analysis, respectively, in micellar media were 0.1-10 and 0.5-5 mg/L for MG and 0.1-15 and 0.5-6 mg/L for BG. The recovery percentages obtained from the analysis of the dyes in real samples of fish-pond water and textile wastewater ranged between 91 and 107%. The good correlation between the results of the colorimetric analysis and the spectrophotometric analysis indicates the reliability of the proposed colorimetric method. Also, the results of the relative recovery study showed insignificant matrix effect. This study demonstrates the ability of the colorimetric analysis coupled with chemometrics tools for simultaneous determination of the analytes even with nearly identical colors.

Estimation of terbinafine HCL by spectophotometric analysis with Tpooo, Nb12br and WFB

One simple and sensitive procedure (simple spectrophotometric method) for the assay of drug Terbinafine HCl in pure form and formulations. This method involves the formation of ion-association complex between TRB and the TPOOO, NB12BR and WFB. In order to establish the optimum conditions necessary for rapid and quantitative formation of coloured product with maximum stability and sensitivity, the author performed experiments by measuring the absorbance at λmax 480nm,420nm and580nm of respective series of solutions, varying one and fixing the other parameters in each case such as type, volume and concentration of acid, organic solvent used for extraction, ratio of organic phase to aqueous phase during extraction, shaking time and temperature. The variable parameters were optimized. The results were statistically validated.

Determination of the Antiprotozoal and Antibacterial Drug Metronidazole in Blood and Dosage Forms Using a Simple Spectrophotometric Method

A new, precise, and sensitive method for the determination of Metronidazole has been created. Metronidazole has been determined in both blood and dosage forms. The reaction is based on the reduction of the nitro group of metronidazole followed by coupling with the diazotized paminodiphenylamine to produce a soluble, colored complex measured at 515 nm. Beer-Lambert law is obeyed over the concentration range from 20 to 100 µg/mL, the molar absorptivity is 1397.88 L/mol.cm.The method has been used for the determination of metronidazole in the blood of the volunteer after 4, 6, 8, and 12 h from the administration of 500 mg tablet showing that the higher level of the drug was found after 6 h from swallowing the drug orally with excellent precision range (RSD% 0.0122-0.0387). The method has been also applied for the estimation of drug content in injection and tablet dosage forms with accuracy ranging from -4.1- to +4.0.

Highly Efficient Spectrophotometric Determination of Cu2+ ion in Aqueous Medium Using a thiosemicarbazone-derivative Ligand.

A new simple spectrophotometric method for the determination of Cu2+ ion was developed using an thiosemicarbazone compound, 2-{4-[Bis(2-chloroethyl)amino]benzylidene}-N-[(4-methylthio)phenyl]hydrazinecarbothioamide (TSC). A simultaneous color change was observed (from colorless to bright yellow) by the addition of Cu2+ ion to the TSC ligand solution. The maximum absorbance of the TSC ligand measured at 366nm was decreased by the presence of Cu2+ ion. The graphs of absorbance obtained by means of the Job's method and the molar-ratio method proposed a complex formation with a 1:2 Cu2+-TSC ligand stoichiometry. The molar-ratio method with emission measurements also confirmed the stoichiometry. The complex stability constant of TSC-Cu2+ complex (K) was evaluated to be 1.76 × 105. The proposed spectrophotometric method was associated with the change in absorbance at 366nm owing to the interaction between the TSC ligand and Cu2+ ion. From the spectrophotometric titration data, it was pointed out that TSC ligand (1.5 × 10- 5mol L-1) selectively reacted with Cu2+ ion in DMSO/water (1:1, v/v, citrate buffer at pH = 6.0). The calibration curve for Cu2+ ion was obtained with a good linearity in the range of 0.0191-0.3241mg L-1. The detection limit for Cu2+ ion was 0.0063mg L-1. The proposed method was achievemently implemented in real water samples (drink water, tap water and, distilled water). Satisfactory recoveries were confirmed at three different concentrations. The method presented a relative standard deviation (RSD%) of less than 3.08%.