Simple Method Research Articles

A Simple Tube Escape Assay to Test Learning and Memory in Zebrafish with Minimized Habituation.

Various methods have been used in rodents to evaluate learning and memory. Although much less frequently used, the zebrafish emerges as an alternative model organism in this context. For example, it allows assessing potential behavioral deficits because of neurodevelopmental disorders or environmental neurotoxins. A variety of learning tasks have been employed in previous studies that required extensive habituation and training sessions. Here, we introduce a simpler and faster method to evaluate learning and memory of zebrafish with minimum habituation. A new apparatus, a transparent L-shaped tube, was developed in which we trained each zebrafish to swim through a long arm and measured the time to swim through this arm. We demonstrate that in this task, zebrafish could acquire both short-term (1 h) and long-term memory (4 days). We also studied learning and memory of a gene knockout (KO) zebrafish that showed social impairments related to autism. We found KO mutant zebrafish to show a quantitative impairment in habituation, learning, and memory performance compared with wild-type control fish. In conclusion, we established a novel learning apparatus and sensitive paradigm that allowed us to evaluate learning and memory of adult zebrafish that required only a brief habituation period and minimal training.

Design of experiments and Derringer's desirability function in optimisation and validation of RP-HPLC method for the analysis of enrofloxacin and its impurities

AbstractUsing the Design of Experiments methodology (Response-Surface Methodology and Derringer's Desirability Function), a simple, fast and robust RP-HPLC method was developed for the analysis of enrofloxacin (EFC), its impurity A (fluoroquinolonic acid, FQ) and impurity B (ciprofloxacin, CPX). Gradient elution of samples was performed on a Zorbax Eclipse XDB C18 column (150 × 4.6 mm, 3.5 μm) with a mobile phase consisting of 32 mM phosphate buffer pH 3.5 – methanol (0 min-19.6% methanol; 15.5 min-19.6% methanol; 29.5 min-80% methanol; 30 min-19.6% methanol; 35 min-19.6% methanol), delivered at a flow rate of 1.5 mL min−1, wavelength of detection 278 nm (for EFX and CFX) and 265 nm for FQ. A good linear response was achieved in the range 15–35 μg mL−1 (EFX) and LOQ-150% for impurities (CFX and FQ). Other validation parameters were also tested: precision, accuracy, sensitivity and robustness. The developed method was shown to be simple, practical and suitable for the analysis of EFC and its impurities (CPX, FQ) in veterinary drugs.

Determination of stearic acid and 12-hydroxyoctadecanoic acid in PEG-60 hydrogenated castor oil by HPLC-ELSD after alkaline hydrolysis

AbstractA rapid and simple method for the determination of stearic acid and 12-hydroxystearic acid in PEG-60 hydrogenated castor oil by high performance liquid chromatography with evaporative light scattering detection was established. The oil sample was first pretreated by alkaline hydrolysis. The analysis was performed on a Zhongpu Develop XD-C18 column (250 mm × 4.6 mm, 5 µm) with gradient elution of methanol and 1% acetic acid aqueous solution at a flow rate of 1.2 mL·min−1 and a column temperature of 40 °C. The drift tube temperature of the evaporative light scattering detection system was set at 40 °C, and the pressure of carrier gas (N2) was 337 kPa. The regression equation revealed a good linear relation (r = 0.9993–0.9995) during the test ranges (119.1–1190.7 μg·mL−1 for 12-hydroxystearic acid, 10.7–107.4 μg·mL−1 for stearic acid). The detection limits of 12-hydroxystearic acid and stearic acid were 1.1 and 2.5 μg·mL−1, the limits of quantitation were 3.2 and 7.4 μg·mL−1, respectively. And the mean recoveries were 101.5 and 101.0%, the corresponding relative standard deviations (RSDs) were 2.1 and 2.8%, respectively. The RSDs corresponding to repeatability (n = 6) were both less than 1.7% in terms of precision. As to the stability, the test results remained stable after 8 h at room temperature (RSDs were both less than 2.6%). The developed method showed high sensitivity, recovery, repeatability and stability, which indicated that the method could be applied as a quality evaluation method for the determination of stearic acid and 12-hydroxyoctadecanoic acid in PEG-60 hydrogenated castor oil.

Sometimes simple is good enough: An analysis of methods for residential building population estimation

AbstractResidential building population data can be useful in a breadth of urban planning, health, transportation, and business applications. Unfortunately, complete datasets of residential building populations are not widely available for use in Canada, and therefore either larger census geographies are used or residential building populations must be estimated. This research explores four different methods of estimating residential building populations, including: an equal allocation method, two measures based on building volume, and a novel method that integrates census data at the dissemination area level to calibrate a population estimation model. This work comprises three parts: 1) a description of these approaches, 2) an evaluation of their validity in a case study in Hamilton, Ontario, and 3) an application of these methods in measuring spatial accessibility to schools. Our results show that most methods yield very similar results, and most provide reasonable estimates of building populations that could be useful for some analytical tasks. However, all methods resulted in instances of error, particularly for the largest population buildings. We conclude that while more complex methods do not significantly outperform simpler methods based on building volume alone, the blend of these methods could yield more accurate population predictions.

Determination of Sulfites in Dried Fruits by Paper Spray Ionization Tandem Mass Spectrometry

Sulfite, a widely used food additive, is subject to regulated labeling. The extraction of sulfite as the stable hydroxymethylsulfonate (HMS) form and its quantitative analysis by liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been recognized for their good sensitivity, selectivity, and versatility across various food materials. This study aimed to develop a cost-effective and simpler method for sulfite quantitation, while maintaining the superior sensitivity and selectivity of mass spectrometry (MS). To achieve this, we introduced paper spray ionization (PSI), an ambient desorption ionization technique that could achieve the direct measurement of analytes without employing separation. We also employed a novel internal standard (IS) structurally similar to the analyte, replacing the more expensive isotopically labeled IS. Although the PSI-MS/MS method developed in this study exhibited slightly lower analytical performance compared to the conventional LC-MS/MS, it remained effective for sulfite analysis in dried fruits.

A Simplified Design Method for the Mechanical Stability of Slit-Shaped Additively Manufactured Reactor Modules

Equipment integrity is an essential aspect of process engineering. Design guidelines facilitate the design and production of safe-to-operate and economic devices. Thin-walled, slit-shaped modules form a subgroup of process engineering devices made via additive manufacturing (AM). Being subject to internal pressure, they have lacked design guidelines until now. We derived a user-centered calculation model for such modules with regular internal structures. It was validated with Finite Element Analysis (FEA) and practical pressure tests for which the modules were manufactured additively. The performance of the calculation could be confirmed, and a design graph was derived. Slit-shaped modules with appropriate internal structures can withstand high pressure at a minimum wall thickness, and they are efficiently fabricated. These structures, being pins, fins, lattice, or heat transfer enhancing fluid-guiding elements (FGEs), occupied approximately 10% of the modules’ internal volume.

Unraveling the intricate fouling behaviors of landfill leachate components during membrane distillation concentration toward zero liquid discharge

Membrane distillation (MD) holds promise for cost-effectively concentrating wastewater toward zero liquid discharge (ZLD), while membrane fouling problems still hinder its practical application, especially for high-strength wastewater containing complex constituents. This study investigated the effects of different foulant components in actual landfill leachate (LFL) on MD deep concentration performances. The fouling behaviors of LFL components were revealed by comprehensively characterizing and analyzing the physicochemical properties of the fouling layers and membrane-foulant interactions. Results illustrated that suspended solids (SS) and macromolecular organics (MO) played significant yet distinct roles in fouling layer formation. SS (>0.45 μm, ∼24 % TOC) possessed strong adhesion energies with membrane surfaces and constructed the main skeleton of the fouling layer at the preliminary concentration stage; while MO (>20 kDa, 0.36 % TOC) deposition resulted in a densely packed fouling layer, influencing the salt crystallization potential and interfering with water and ammonia transport. In contrast, the low-molecular organics with the highest content (<20 kDa, ∼75 % TOC) were mainly electron-donor monopolar substances weakly adsorbed on the nonpolar membrane surface. Removing SS and MO from LFL notably prevented fouling layer formation and maintained high membrane efficacies (water flux decline mitigated from ∼50 % to ∼15 % and ammonia rejection increased from 71 % to 88 %), even as water recovery increased to 95 %. The findings of this work imply conventional anti-fouling strategies (e.g., complete removal or degradation of organic components) may have gone beyond what is necessary, and will inspire the development of simpler and greener methods to maintain membrane efficacy during high-strength wastewater treatment toward ZLD.

A Simplified Method for Determining Fire Resistance of RC Columns Using Fire-Resistance-Column-Curves Approach

A Simplified Method for Determining Fire Resistance of RC Columns Using Fire-Resistance-Column-Curves Approach

Порівняльна оцінка собівартостей відпуску електроенергії з різних джерел в базовому та регулювальному режимах

The paper analyses the role and the technical and economic indicators of different electricity generation sources in the transition to "carbon-free" energy. A simplified method is proposed for estimating the minimally justified price of selling electricity from thermal power plants (TPPs), renewable energy sources (RES) and new maneuverable natural gas capacities is proposed. It is shown that the price of electricity from RES and new maneuverable capacity has a decisive influence on the amount and fixed period of return on investment in construction, as well as on the capacity utilization factor (CUF), which depends on the mode of operation of the power plants. Comparative estimates of the CUF of different electricity generation sources are presented. It has been shown that the cost of electricity from RES, new peak and maneuverable capacity is significantly higher than the cost of electricity from thermal power plants during the payback period. It is proved that at present the reserve for the reduction of the power shortage and the load regulation is available only in the thermal generation, and the conditions for the possibility of its effective use are the maintenance of the proper technical condition of the pulverized coal boiler units and the sufficient fuel base. To ensure acceptable electricity prices, RES and new control capacity should be phased in, using existing TPPs and CHPs to balance both the weighted average price and the regulation of power system load modes. Keywords: thermal power plants, coal, natural gas, gas turbine, steam-gas, gas-piston power plants, renewable energy sources, cost of electricity supply.

A market driven identification of current and emerging skills requiring an MBA degree: a topic modelling approach

ABSTRACT The emerging field of Labour Market Intelligence focuses on extracting real-time information for professional skills. Online job posting websites provide unique opportunities to gather such intelligence, useful for job seekers and academic administrators in charge of preparing the future labour force. We propose a probabilistic topic modelling approach, incorporating occupational ontologies, to extract skillsets required for jobs needing a Master of Business Administration (MBA) degree. Analysing LinkedIn job postings in Pennsylvania, USA, we identified 28 job functions and associated skillsets confirming known demands and revealing new ones not yet identified in government and private labour market reports. By aggregating these skills, we can align MBA curricula with market needs, offering a superior alternative to conventional surveys. Our results are comparable with other popular neural models. Our approach provides improved computational efficiency with simpler methods Additionally, this methodology can be applied globally in other fields with existing occupational ontologies.

Seismic performance of recentering energy dissipation bracing with pendulum in prefabricated steel frame structure systems

This research introduces the recentering energy dissipation bracing with a pendulum in prefabricated steel frame (REBP-PSF) structures. The primary objective of this study is to mitigate potential severe damage to steel frames during seismic events and to expedite postearthquake rehabilitation. This innovative system harnesses energy dissipation through friction between structural members and facilitates recentering through the cable pretensioning force. Consequently, this may make postearthquake repairs minimal or even unnecessary. Three REBP-PSF structures and a prefabricated steel frame with cantilever beam splices (PSF-CBS) were meticulously modeled using the ABAQUS finite element method to evaluate the seismic performance of this novel system. The study investigated the effects of pretensioning parameters and varied structural morphologies on seismic performance metrics, including failure modes, hysteresis performance, energy dissipation capacity, and residual displacements. This paper introduced a simplified method to simulate the restoring force model of REBP using the connection element method. Subsequently, a model for the REBP-RSF overall structure was established, enabling nonlinear dynamic time-history analysis, which was then compared with the rigid steel frame (RSF) structure. The results showed that the REBP-PSF structure outperformed the PSF-CBS in terms of initial stiffness, yield load, ultimate load, and energy dissipation capability. The bearing and energy dissipation capacities of the REBP-PSF structure were enhanced by augmenting the cable pretensioning force within this structure. The proposed connection element method could accurately simulate the hysteretic behavior of REBP. Compared with the RSF structure, the REBP-RSF demonstrated superior seismic performance, characterized by small story drift and residual displacements during seismic activities. Furthermore, a notable augmentation was observed in the lateral stiffness and torsional rigidity of the proposed structure.

Regional noise source location based on the time delays between station pairs from ambient noise interferometry

Identifying the location of a potential noise source assists in understanding the characteristics of the seismic or volcanic activity and provides valuable information for hazard assessment. Unlike the conventional waveform-based techniques that rebuild the source energy into the possible source region, we apply a simplified method to determine the absolute location of the noise source based on the station-pair time-delays from ambient noise interferometry. Synthetic tests demonstrate the robustness of the method and the locating precision is mainly influenced by the signal-to-noise ratio of the synthetic waveforms, and the higher frequency bandwidth source signals are more likely to result in accurate detection of the source. An application at the Central Tien Shan indicates that our method is capable of locating the known virtual source from the empirical Green’s functions. Furthermore, assuming a surface wave velocity, the depth of the source can be generally recovered from ambient noise interferometry in a simplified 3-D homogeneous model. The new method sheds light on applications of ambient noise interferometry for locating potential sources, making it suitable for detecting time-dependent behavior.

Comparison of two methods: Clipping and suturing in laparoscopic appendectomy

ObjectiveClosure of the appendix stump after appendectomy is considered one of the most important parts of laparoscopic appendectomy. Various techniques are used during this surgery, commonly including endoclips and ligatures. Therefore, this study was conducted to compare endoclips with intra-corporeal ligatures in closing the appendix stump during laparoscopic appendectomy. MethodsThis retrospective study was conducted using data from 50 patients with acute appendicitis who underwent uncomplicated laparoscopic appendectomy. The data from patients whose stumps were closed using endoclips in 25 patients (Group I) and with intra-corporeal ligatures in 25 patients (Group II) were extracted and compared regarding the occurrence of intraoperative and postoperative complications. ResultsThere were no differences between the two methods in terms of intraoperative and postoperative complications. The duration of surgery in the ligature group was significantly longer than in the clip group (p = 0.044). The hospital stay duration was clinically longer in the ligature group, but this difference was not statistically significant (p > 0.05). ConclusionEndoclips for closing the appendix stump are safer with a shorter operating time and also a simpler method. Therefore, they could be a reliable alternative to the method of closing the appendix stump with ligatures.

Bleed air CFD modelling in aerodynamic simulation of A heavy duty gas turbine compressor

This study investigates the effects of different bleed air CFD modelling methods on the aerodynamic simulation of a heavy-duty gas turbine multistage compressor with bleed airflow. Two methods, the local source term method and the simplified bleed off-take geometry method, are compared. The results reveal that while overall compressor performance shows minimal variation between the methods, significant differences are observed near the blade tip region. The bleed source term method predicts a more uniform flow field in the bleed cavity, leading to a larger surge margin for the compressor. Therefore, in the analysis of multistage compressors, it is advisable to adopt simplified geometric modeling method for simulating bleed air extraction. Moreover, when utilizing this approach, careful consideration should be given to the choice of the slot inclination angle. This design parameter presents a crucial trade-off between losses within the exhaust structure and those arising from the entry of mainstream flow into the exhaust slot cavity.

Application of the fast 3D simplified simulation method for the large CAP1400 nuclear island evaporator based on the coupled source term method

As for the evaporator of the large CAP1400 nuclear power plant, it has a large volume with numerous tubes along with the complex flow heat transfer phenomenon. The traditional direct simulation method usually takes a lot of time and computing resources to obtain the flow heat transfer data. To solve the problem, a fast simulation method established on the porous medium source term was herein proposed. In this simplified simulation method, the complex evaporator model is replaced with the porous medium model, where the derived heat and momentum source terms are added into the governing equation to control the heat flows. Five kinds of simplified evaporator models with different numbers of pipes were set to be the research objects, the internal flows inside the evaporator were studied with the traditional direct simulation method and the newly proposed simplified simulation method. Via comparative analysis, it can be found that: 1) The newly proposed simplified simulation method accurately captures the distribution characteristics of pressure, temperature, and entropy production in the evaporator, serving as a viable alternative to traditional direct simulation methods. Specifically, the average simulation errors for inlet-outlet pressure difference and temperature difference are both less than 5 % and 7 %, respectively. 2) The newly proposed simplified simulation method can capture the mutual interference effects between the evaporator and the nuclear main pump. When the evaporator is connected in series with the main nuclear pump, the average head of the main pump increases by 0.34 m, while the efficiency decreases by an average of 0.34 %. The average simulation errors of the simplified simulation method are 0.06 m and 0.13 %, respectively. 3) The application of the simplified simulation method leads to a significant 85 % decrease in the average computational cost for evaporator calculations and a notable 36 % decrease for combined calculations of the evaporator and main pump. The study here is expected to provide technique support for the simulation and evaluation of the large nuclear island.

A Comparative Study on Load Assessment Methods for Offshore Wind Turbines Using a Simplified Method and OpenFAST Simulations

Simplified methods are often used for load estimations during the initial design of the foundations of offshore wind turbines (OWTs). However, the reliability of simplified methods for designing different OWTs needs to be studied. This paper provides a comparative study to evaluate the reliability of simplified approaches. The foundation loads are calculated for OWTs at the mudline level using a simplified approach and OpenFAST simulations and compared. Three OWTs, NREL 5 MW, DTU 10 MW, and IEA 15 MW, are used as reference models. An Extreme Turbulence Model wind load at a rated wind speed, combined with a 50-year Extreme Wave Height (EWH) and Extreme Operating Gust (EOG) wind load and a 1-year maximum wave height are used as the load combinations in this study. In addition, the extreme loads are calculated using both approaches for various metocean data from five different wind farms. Further, the pile penetration lengths calculated using the mudline loads via two methods are compared. The results show that the simplified method provides conservative results for the estimated loads compared to the OpenFAST results, where the extent of conservativism is studied. For example, the bending moment and shear force at the mudline using the simplified approach are 23% to 69% and 32% to 53% higher compared to the OpenFAST results, respectively. In addition, the results show that the simplified approach can be effectively used during the initial phases of monopile foundation design by using factors such as 1.5 and 2 for the shear force and bending moment, respectively.

A simplified mathematical modeling approach for thermal runaway of Li(Ni0.8Co0.1Mn0.1)O2 pouch cells based on thermal runaway experimental data

Currently, the numerical modeling methods used to characterize the thermal runaway and propagation mechanisms in NCM811 pouch batteries still face numerous challenges. This is primarily due to the uncertainties in thermal runaway reactions and the immense computational demands encountered when modeling begins at the granular level of the cell. To address this issue, this study designed a series of experiments for an NCM811 pouch battery. By employing experimental data, reasonable assumptions were made regarding the combined effects of solid-state thermal conduction, electrochemical heating, and fluid-solid heat transfer through gas jet streams. A simplified thermal runaway mathematical modeling method based on experimental data was proposed, effectively tackling the problem. This framework was then applied to thermal runaway experiments on a single NCM811 battery used in this study, providing essential input parameters for three-dimensional numerical modeling of modules composed of multiple batteries in multiphysics simulation software. The simulation results derived from the proposed method were in good agreement with experimental data. This study can characterize the thermal runaway and thermal propagation characteristics of battery modules using single-cell thermal runaway experiments. Moreover, the proposed modeling approach not only predicted the experimental outcomes of the NCM811 pouch battery with reasonable accuracy but also provided smoother data, elucidating the highly fluctuating trends observed in experimental data. This method also offers guidance for designing fire prevention and smoke exhaust systems for battery packs. This study presents a novel approach for the mathematical modeling of thermal runaway and thermal propagation in battery modules using NCM811 pouch batteries, contributing to the ongoing development of lithium-ion battery module design.

Abstract PO3-22-10: 3D-CT mapping with a photo-translucent application using iPad delineates tumor lesions on patient breast skin in breast-conserving surgery: Preliminary results

Abstract Objective: The aim of this study was to evaluate the feasibility and efficacy of a 3D-CT mapping technique with a photo-translucent application using an iPad for tumor localization on the patient's breast skin in breast-conserving surgery (BCS). We also compared the accuracy of tumor localization between conventional methods and 3D-CT mapping. Methods: Between February and July 2023, 20 female patients with early-stage (0 to 2) breast cancer scheduled for BCS were enrolled in this study. Preoperative enhanced CT scans were performed, and a 3D-CT image was generated by incorporating the patient's skin and manually marking the contrast-enhanced lesion using SYNAPSE VINCENT software. The 3D-CT images, adjusted to individual rotation angles to match the horizontal skin position above the tumor, were imported into an iPad equipped with a photo-translucent application. Using a securely fixed iPad holder, the translucent image was superimposed on the patient's breast, and the enhanced lesion was marked on the skin using a blue pen. Conventional tumor marking was performed based on ultrasound imaging, CT, and MRI findings using a black pen. Photographs were taken to simulate BCS based on the 3D-CT blue marking. All patients underwent BCS with resection margins of 1.5-2 cm from the black marking, and intraoperative frozen section analysis was performed to assess the resection margins. Results: The majority of patients had hormone receptor-positive breast cancers, and neoadjuvant chemotherapy was administered in three patients (Table 1). The extent of tumor involvement assessed by pathological assessment and imaging findings showed the least discrepancy with MRI, with an average error of 4.2 mm. MRI also had the lowest rate of missed tumor detection compared to other imaging modalities. The initial positive margin rate was 20%, and the simulation conducted in this study did not alter that value. Furthermore, the simulation demonstrated more effective excision of the tumor's center compared to conventional surgical methods (Table 2). Conclusion: The use of 3D-CT mapping with a photo-translucent application on an iPad offers a promising approach for accurate tumor localization in BCS. This technique provides a simplified and efficient method, and it can also be applied to cases where lesions have completely disappeared after neoadjuvant chemotherapy. XX- XX XX- XX Citation Format: Gou Watanabe, Akihiko Suzuki. 3D-CT mapping with a photo-translucent application using iPad delineates tumor lesions on patient breast skin in breast-conserving surgery: Preliminary results [abstract]. In: Proceedings of the 2023 San Antonio Breast Cancer Symposium; 2023 Dec 5-9; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2024;84(9 Suppl):Abstract nr PO3-22-10.

Simplified Coupled Calculation Method for Aeroelasticity of Large Flexible Aircraft

Large flexible aircraft have the characteristics of large wingspan, low wing load, slow flight speed, and significant impact from gust loads. Therefore, the load and response issues of such aircraft have received widespread attention from scholars at home and abroad. This article establishes a simplified coupled calculation method for gust loads on large flexible aircraft, achieving coupled calculations of flight motion, structural deformation, and aerodynamic changes at different time steps. Due to the simple strip method used for aerodynamic modeling, this method is relatively fast compared to the CFD-CSD calculation method. In order to verify the accuracy of the method, the calculation results were compared with those of the standard calculation method. The comparative calculation results show that the simplified coupling calculation method is relatively accurate for this calculation example and can play an important role in engineering experiments with low requirements for calculation results.

TRM strengthening as effective prevention against premature failure of masonry walls

Masonry structures are very prone to cracking and premature failure in the presence of shear loading. Therefore, effective prevention of their premature damage is needed. This paper presents laboratory tests of small models made of autoclaved aerated concrete (AAC) blocks, which were externally reinforced using two different strengthening sets consisting of mortar and mesh, and subjected to diagonal compression. The experiments used a commercialised system designed for the strengthening of typical masonry substrates (brick, stone) and a low-cost and lowstrength mesh and mortar set used as part of a system for insulating buildings. The analyses presented are of a comparative nature and describe changes in the behaviour of the models depending on the strengthening set used. The application of full-surface strengthening clearly changed the behaviour of AAC walls from brittle one to the ‘pseudo-ductile’ one observed after the appearance of the first cracks. In all strengthened models, a significant increase in the load capacity (from 3% to 62%) and a post-peak phase were reported. An interesting finding was the high efficiency of nonstructural materials (plastering system), comparable to the effects of the use of commercialised system solutions. This strengthening can be successfully considered as an effective preventive action. Finally, a simplified method is provided to calculate the contribution of the strengthening to the load bearing capacity of the strengthened models. The method defines the effect of the mesh and the mortar independently, based only on the basic material properties of these two components.