Significant Amount Research Articles

Spatio-Temporal Photovoltaic Power Prediction with Fourier Graph Neural Network

The strong development of distributed energy sources has become one of the most important measures for low-carbon development worldwide. With a significant quantity of photovoltaic (PV) power generation being integrated to the grid, accurate and efficient prediction of PV power generation is an essential guarantee for the security and stability of the electricity grid. Due to the shortage of data from PV stations and the influence of weather, it is difficult to obtain satisfactory performance for accurate PV power prediction. In this regard, we present a PV power forecasting model based on a Fourier graph neural network (FourierGNN). Firstly, the hypervariable graph is constructed by considering the electricity and weather data of neighbouring PV plants as nodes, respectively. The hypervariance graph is then transformed in Fourier space to capture the spatio-temporal dependence among the nodes via the discrete Fourier transform. The multilayer Fourier graph operator (FGO) can be further exploited for spatio-temporal dependence information. Experiments carried out at six photovoltaic plants show that the presented approach enables the optimal performance to be obtained by adequately exploiting the spatio-temporal information.

PROXIMATE, MINERAL AND PHYTOCHEMICAL ANALYSIS OF SOME MEDICINAL PLANTS COLLECTED FROM ORATHUR VILLAGE, THIRUPORUR TALUK KANCHEEPURAM DISTRICT TAMILNADU, INDIA

The use of medicinal plants has a long tradition as part of the human diet and has therapeutic agents. That is why they are well accepted by consumers and are generally considered to be environmentally friendly, efficient and generally considered to be safe alternative to antibiotics due to the presence of phytochemicals found in the leaves, stem bark, roots, flowers amongst others. Medicinal plant also possesses nutritional properties and play multiple pharmacological roles such as anti-inflammatory, antioxidant, anti-cancer, antimicrobial, anti-viral, anti-fungal, anti-helminthic, hepato-protective activity amongst others. This study was aimed at accessing the proximate, mineral and phytochemical composition of Tephrosia purpurea, Adhatoda vasica, Crataeva nurvala, Bauhinia racemose, Holarrhena antidysenterica and Aegle marmelos. Result on proximate analysis showed that moisture content varied from 13.57 – 16.02 %, dry matter (83.98 – 86.43 %), crude protein (5.06 – 7.12 %), ash (9.90 – 12.04 %), crude fibre (12.74 – 14.32 %), crude fat (0.10 – 0.22 %) and carbohydrates (40.30 – 50.17 %). Concentrations of calcium, phosphorus, potassium, magnesium, zinc, manganese, sodium and copper ranged from 120 – 148.0 mg/100g, 180.0 – 300 mg/100g, 66.8 – 94.2 mg/100g, 41.8 – 69.8 mg/100g, 27.9 – 44.6 mg/100g, 119 – 133 mg/100g and 8.21 – 13.1 mg/100g respectively. Quantitative evaluation of tannins showed that values varied from 39.00 – 46.50 mg/g, alkaloids (26.35 – 35.09 mg/g), saponins (20.05 – 30.40 mg/g), flavonoids (66.10 – 86.11 mg/g), terpenoids (40.71 – 45.88 mg/g) and phenols (106.8 – 125.4 mg/g). In conclusion, it was discovered that it contained some essential nutrients in significant quantities. However, values obtained varied due to age of plant, specie, processing method among others. The presence of phytochemicals in these plant suggests that it has pharmacological properties. Accessing the constituent’s in this plants could lead to a novel drug discovery and curb the increasing cases in antimicrobial resistance.

Skin as outermost immune organ of vertebrates that elicits robust early immune responses after immunization with glycoprotein of spring viraemia of carp virus.

As the outermost immune organ in vertebrates, the skin serves as the primary interface with the external environment and plays a crucial role in initiating the early immune response. The skin contains a variety of immune cells that induce mucosal and systemic immune responses, rendering it a prime target for vaccination strategies. Insight into the mechanisms through which vaccination triggers early immune responses is paramount for advancing animal and human health, yet our current understanding remains limited. Given its significance in vertebrate evolution, teleost fish emerges as an excellent model for investigating the early immune response of skin. In this study, we demonstrate that significant quantities of vaccine can be absorbed by the skin and transported to the body through dermis and muscle metabolism by immerses immune zebrafish with glycoprotein of spring viraemia of carp virus. Immersion immunization can elicit robust and enduring immune protection, with the skin triggering a potent immune response early in the immunization process. Analysis of the skin transcriptome revealed the involvement of numerous immune-related genes in the immersion immune response, with indications that HSP70 and MAPK signals might play pivotal roles in the immune process induced by glycoprotein. Co-immunoprecipitation and cell co-localization studies confirmed the interaction between glycoprotein and HSP70. Subsequent research demonstrated that overexpression or inhibition of HSP70 could respectively enhance or impede the expression of JNK and related proteins. However, the survival rate and immune response of HSP70 inhibited zebrafish with glycoprotein treatment were significantly reduced. These findings propose that the interaction between glycoprotein and HSP70 may activate JNK, thereby modulating mucosal and systemic immune responses induced by glycoprotein. This investigation offers novel insights and a foundational understanding of early skin immune reactions.

Asphaltenes biodegradation from heavy crude oils by the yeast Yarrowia lipolytica.

Heavy crude oil reserves are characterized by their high viscosity and density, largely due to significant quantities of asphaltenes. The removal of asphaltene precipitates from oil industry installations is crucial, as they can contaminate catalysts and obstruct pipelines. Therefore, this study aimed to bio-transform heavy oil asphaltenes into smaller molecules using the yeast Yarrowia lipolytica, known for its ability to efficiently degrade hydrophobic substrates. For this purpose, asphaltenes were extracted from crude oil samples, and yeast growth was assessed in a mineral medium containing 2, 5, or 10g L-1 of asphaltenes. After 168h of incubation, liquid-liquid extraction was conducted on samples from the Yarrowia lipolytica growth medium using chloroform. The extracted fractions were then quantified by gas chromatography. The results indicated that the yeast could utilize the asphaltenes as a carbon source for growth, though there was a delay in growth compared to the control (glucose as the carbon source), with a maximum biomass concentration of 2.26g L-1 achieved at 144h. From the experimental design, it was determined that a higher concentration of aromatic compounds was achieved under the conditions of 115rpm, 2g L-1 of asphaltenes, and 0.5g L-1 of cell inoculum. Conversely, to obtain a higher concentration of saturated compounds, the optimal conditions were 160rpm, 5g L-1 of asphaltenes, and 1.0g L-1 of cell inoculum. Molecular docking results indicated that asphaltenes have a high affinity for cytochrome P450, laccase, and Lip2, with interactions observed with their catalytic triads, suggesting a significant role for these enzymes in asphaltene bioconversion.

Cosmogenic 21Ne exposure ages on late Pleistocene moraines in Lassen Volcanic National Park, California, USA

Abstract. We report new cosmogenic 21Ne in quartz exposure ages from 18 samples on three distinct moraines deposited in the Lost Creek drainage, approximately 3–7 km down-valley from Lassen Peak in Lassen Volcanic National Park. Although measuring 21Ne in quartz is generally straightforward, accurate 21Ne exposure dating of deposits of late Pleistocene is rarely possible due to the significant quantities of non-cosmogenic 21Ne present in most lithologies. Young quartz-bearing volcanic rocks have been observed to be an exception. We take advantage of moraine boulders sourced from the ∼ 28 ka dacite of Lassen Peak to generate a chronology of alpine deglaciation in Lassen Volcanic National Park. Ages from three distinct moraines are in stratigraphic order at 22.1 ± 3.8, 20.2 ± 2.4, and 15.3 ± 3.8 ka and generally agree with other terminal and some recessional moraine ages across the Cascade Range and Sierra Nevada of the western United States. To date, these are among the youngest surfaces ever dated using cosmogenic 21Ne and provide a cost-effective proof-of-concept approach to dating moraines where applicable.

Структурa і властивості матеріалів Fe—Al—Ga в області концентрацій, збагачених залізом

The structure and mechanical properties of materials of the Fe—Al—Ga system (in the concentration range of 78—82% (wt.) Fe), obtained by sintering at 1150 oC in Ar medium for 1 hour, were studied. The starting materials were mixtures of Fe, Al powders and crushed Fe—Ga ligature alloy of equiatomic composition. Seven mixtures were produced, of which two mixtures had a binare compo¬sition (% (wt.)): Fe—17,5Al and Fe—21,4Ga; and all the others were three-component, with gradual substitution of Al for Ga. The phase composition of the samples was studied by the methods of X-ray diffraction and local X-ray microspectral analyses. To determine the mechanical characteristics, the Brinell method was used, microdurometric studies were carried out, and mechanical tests of the samples were carried out under conditions of uniaxial compression. The effect of Al on the change in the porosity of Fe—Al—Ga ternary alloys and the maximum manifestation of the “swelling” effect for Fe—Al composition samples was established. It is shown that the main phase of the obtained materials is a solid solution based on Fe, in which Al and Ga are dissolved in the appropriate proportions. In the Fe—Al samples and in the samples of the ternary composition, there are local separations of phases containing, in addition to the main metals, carbon and oxygen in significant quantities. In the structure of Fe—Ga samples, carbon is not detected, and oxygen is present in a small amount. The results of the mechanical compression tests showed that the Fe—21,4Ga composition samples, like the pure Fe samples, are quite plastic and do not break up to a degree of compression of 75—82%. For materials with binary composition with Ga, an increase in the elastic range was recorded compared to pure Fe, and the replacement of a small proportion of Ga with Al (up to 2% by weight) in the material composition contributes to its strengthening. Keywords: Fe, Al, Ga, microstructure, phase formation, microhardness, mechanical compression characteristics.

Assessing the Efficacy of the Plasma Spinning Disc Reactor (PSDR) in Treating Undiluted Aqueous Film Forming Foams (AFFFs)

This work used pulsed electrical discharge plasma to treat undiluted Aqueous Film Forming Foam (AFFF) solution that contained significant quantities of per- and polyfluoroalkyl substances (PFAS). The plasma was generated within a plasma spinning disc reactor (PSDR), which utilizes the electric breakdown of argon gas to create plasma over a thin liquid film generated on a spinning disc.The PSDR performance toward degradation of AFFF constituents such as fluorotelomers, perfluorinated C2–C7 alkyl acids, and unidentified precursors was investigated. The PSDR performance sensitivity to the process parameters, such as reactor electrode design, disc rotational speed, liquid flowrate across the disc, and discharge voltage, was explored. The results indicate degradation of all quantified PFAS with chain length ≥ 4 to below detection limit within a 250mL sample after 86hours of treatment on a 6.5cm disc rotating at 200rpm. The overall PSDR performance was insensitive to the tested process parameters. A direct comparison with a UV/H2O2 process revealed the superior performance of the plasma-based treatment toward degradation of perfluorinated compounds, evidenced by higher precursor removal rates and lower energy consumption. The energy efficiency of the UV/H2O2 system was approximately 100 times lower than that of the plasma process under all conditions. This study confirms that PSDR is a promising technology for effectively remediating PFAS in undiluted AFFF.

Physicochemical Characterization of Chitosan-Multiwalled Carbon Nanotubes Hybrid Material for Ofloxacin Delivery

The present study involved the synthesis of a bioactive composite material using chitosan (CS) and multi-walled carbon nanotubes (MWCNTs) through the free radical polymerization method. The resulting composite material was comprehensively characterized using various experimental techniques, such as Fourier-transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD). The quantification of the swelling behavior involved the measurement of changes in the weight of the sample as a function of the time it was immersed in an aqueous buffered solution. The findings indicated that the maximum drug-loading rate of ofloxacin medication (OFL) was 86%. Moreover, the pH sensitivity of the poly acrylic acid grafting multi-walled carbon nanotubes p(CS-co-AA)/MWCNTs nanocomposite facilitated the substantial release of a significant quantity of medication in aqueous buffered solution at a pH of 1.2. The average rate of drug release was measured to be 76% after 72 h duration. On the other hand, the release of the drug at pH 5 and 7.4 was 42% and 32%, respectively. According to the reported findings, the p(CS-co-AA)/MWCNTs carrier has a favorable capacity to control the release of the OFL drug into the intended medium while reducing potential negative effects.

Sustainable Management of Green Waste in Urban Settings: A Case Study on Energy Recovery and Heating Solutions in the Municipality of Athens (Greece)

The increasing volume of municipal solid waste (MSW), including biodegradable plant residues such as pruning, leaf, and kitchen wastes, presents a substantial environmental challenge due to the limited availability of landfill space and the resulting environmental contamination. Sustainable waste management practices, encompassing recycling and waste-to-energy conversion through biological or thermochemical processes, are imperative. In the Municipality of Athens, Greece, significant quantities of green waste generated from public and private gardening activities provide a valuable opportunity for energy recovery and landfill waste reduction. In accordance with Directive 2008/98/EC, Athens emphasizes waste prevention, reuse, recycling, and recovery. This study examined alternative bio-waste and green waste management systems, using examples from Europe, focusing on the Athens Directorate of Urban Green Spaces and Urban Wildlife. This paper discusses methods for assessing the energy value of pruning residues, providing a definitive disposal framework. Additionally, it presents a technoeconomic study of one of the municipal swimming pools in the Municipality of Athens, investigating the production and distribution of thermal energy to meet the heating needs of the pool facilities. This research identified key constraints and their impact on decision-making, highlighting the potential for alternative green waste management strategies. It advocates modern recycling techniques in line with national and community legislation, which have significant environmental and economic benefits.

Environmental impact of pediatric ENT surgery: A STROBE analysis

IntroductionIncreasing emissions of greenhouse gases contribute to climate change. The healthcare sector, and particularly the operating room, is a significant emitter of greenhouse gases. In head-and-neck surgery, pediatric procedures are very common, but few studies have assessed their ecological impact. Our objective was to quantify the carbon footprint of common pediatric head-and-neck surgeries (tonsillectomy, adenoidectomy, and transtympanic tube placement). MethodsAn eco-epidemiological study was conducted in December 2022 in a tertiary hospital center. The carbon footprint of 10 pediatric head-and-neck surgeries (tonsillectomies, adenoidectomies, transtympanic tube placements) was calculated, as the sum of carbon dioxide (CO2eq) emissions generated by patient and medical staff transport, waste production, energy consumption in operating rooms, manufacturing and transport of disposable and reusable medical devices (MDs), medication production, and sterilization of reusable MDs. ResultsThe carbon footprint of 1 pediatric head-and-neck surgery was 57.86kgCO2eq. Disposable MDs were the most polluting item with 30.82kgCO2eq (53.3%). Patient transport accounted for 27.4%, medication for 12.6% and reusable MDs for 2.9%. ConclusionsPediatric head-and-neck surgeries generate a significant quantity of CO2, mostly due to the production and delivery of disposable MDs. These observations could serve as a starting point for ecological actions consistent with an environmentally sustainable and climate-resilient health system.

A ratiometric fluorescent biosensor based on catalytic hairpin assembly reaction for ultrasensitive detection of aflatoxin-producing genes

Aflatoxins as the world’s most toxic contamination is metabolized by Aspergillus flavus and its production is closely related to aflatoxin-producing genes. It is significant to monitor the aflatoxin-producing genes as early as possible to prevent the contamination and spread of aflatoxins. Therefore, a label-free and free separation fluorescent biosensor using the selective cation exchange reaction in conjunction with the signal amplification of catalytic hairpin assembly (CHA) was developed for the detection of aflatoxin-producing gene utilizing CdTe quantum dots (CdTe QDs) and Nitrogen-doped carbon quantum dots (NCQDs) as the beacon molecule. There were two types of hairpin structures in this sensing system, hairpin probe 1 (HP1) was generated by Ag+ and the recognition probe with the complex C-Ag+-C structure and hairpin probe 2 (HP2) was the DNA fragments showing a hairpin arrangement. Target nor-1 gene may activate the CHA, which created a significant quantity of HP1-HP2 double-stranded DNAand recycled the target nor-1 gene. The released amounts of Ag+ suppressed the fluorescence signal of CdTe QDs, while it wasn’t observed the discernible effect on NCQDs. This fluorescent biosensor displayed the excellent sensitivity for aflatoxin-producing nor-1 gene in a dynamic range of 5 pM to 50 nM with a detection limit of 1.66 pM. This fabricated sensor also showed the visible distinguish for different concentrations of nor-1 gene. It exhibited the potential to offer vital enhancements for food quality control through a simple, fast and sensitive monitoring test system.

Evaluation of tol laut in Tahuna and Natuna as underdeveloped, remote, outermost, and border areas

Abstract Tahuna (North Sulawesi) and Natuna (Riau Islands) are included in the 3TP (underdeveloped, remote, outermost, and border) areas and use sea transportation to support for their economic activities. High inflation and price disparities are economic problems in this area. “Tol Laut” is government initiative designed to distribute essential basic goods, through a subsidized sea transportation system. These routes connect the economic hub in Java with the 3TP areas. The analysis process uses a mix method, quantitative and qualitative analysis. Port Assessment analysis using weighted scoring, Supply Demand Analysis using index consumption demand, and Regulation Review. To evaluate the implementation of Tol Laut, it focuses on aspects of port location, connectivity, port facilities, potential feeder ships, production performance, hinterland, as well as administration and commerce. Tol Laut in Tahuna can fulfill the supply-demand of essential basic goods significant quantities around 30% from local demand and is supported by the regional government’s commitment, allowing Tol Laut to reduce price disparities. On the contrary, Tol Laut in Natuna can only the supply 16%, resulting inability to control price disparities effectively. There is an urge to optimize and coordinate regarding Tol Laut by involving agencies for the successful enforcement of regulations.

Mechanistic investigation on degradation of tetrabromobisphenol S by OH, O3 and SO4•- in wastewater and atmosphere

Human cytotoxicity and neurotoxicity are two characteristics of the new brominated flame retardant (NBFR) tetrabromobisphenol S (TBBPS). TBBPS can be discovered in both water and the atmosphere. The degradation of TBBPS by •OH and O3 both in the atmosphere and water was investigated. For the first time, we examine the mechanism of TBBPS and O2•- transformation using the density functional theory (DFT) method. We also examine the routes of TBBPS with SO4•-, and 1O2. The TBBPS-OH intermediates will react with O2 and HO2• to produce products in water, and the •OH-addition products of TBBPS will react with O2 and NO in the atmosphere. In comparison to the water phase, the gas phase TBBPS pathways with •OH and O3 are more advantageous. At 298K, the TBBPS reaction rate constant with O2•- is 5.39 × 10-7M-1 s-1. The TBBPS O2•--addition routes are superior to the TBBPS O2•--SET pathways. SO4•- and •OH predominate in the breakdown of TBBPS, where O2•-, 1O2 and O3 playing a minor role. Significant quantities of very hazardous TBBPS breakdown products are produced by SO4•--based AOPs. TBBPS has an environmental half-life of 0.0119s within the [•OH] of 10-9molL-1 in AOPs at 298K, despite being environmentally persistent in natural waters. The majority of TBBPS transformation products have far lower levels of toxicity. This work disproved the theory that O2•--based AOPs degrade TBBPS and supported the degradation of TBBPS by AOPs based on •OH.

Comparison of two automated urine cytometry systems: Sysmex® UF-1000i and Beckman Coulter® DxU 850 Iris

Background: Urinary tract infection, defined as the presence of bacteria or yeast in the urinary tract, is the most common community-acquired infection after respiratory infections. The cytobacteriological examination of urine (CBEU) remains the primary diagnostic test for urinary tract infections and is the most frequently conducted test in microbiology laboratories. Direct examination is a crucial step of CBEU, enabling the assessment of cytology, including leukocytes and red blood cells, as well as the identification of crystals, epithelial cells, and microorganisms when present in significant quantities. This examination also provides preliminary results that can guide clinical decision-making. While the standard method for urine cytology is a microscopic examination, automation offers several advantages, including standardized results with higher repeatability, improved reproducibility, increased sample throughput, and seamless data transfer to laboratory information systems. Objectives: This study aimed to compare the performance of two automated urine cytology systems: Sysmex UF-1000i and the Beckman Coulter DxU 850 Iris. Methods: We described the methodology and technology underlying each system and assessed their analytical performance. The UF-1000i uses flow cytometry for the objective characterization and identification of particles based on forward scattering, fluorescence, and adaptive typing analysis. In contrast, the DxU-850 Iris, a urine microscopy analyzer, employs proprietary digital flow morphology technology alongside automatic particle recognition software to isolate, identify, and characterize digital images of particles. Conclusion: Our comparison showed that both systems performed exceptionally well, delivering results that are comparable, and, in some cases, superior to, those obtained through the reference method of optical microscopy.

Trema micranthum (L.) Blume as a new source of cannabinoids

Trema micranthum (Cannabaceae) has emerged as a promising new source of cannabinoids, including cannabidiol (CBD). Given the substantial medicinal demand for cannabinoids and the regulatory challenges associated with Cannabis sativa due to the presence of Δ9-tetrahydrocannabinol (THC), this study sought to explore the presence of CBD, THC, and their precursors, Δ9-tetrahydrocannabinolic acid A (THCA A) and cannabidiolic acid (CBDA), in various parts of Trema micranthum using UHPLC-HRMS/MS (Orbitrap). Extracts from fruits, leaves, inflorescences, and stems were obtained using a methanol/hexane (9:1, v/v) solvent mixture. UHPLC coupled with an Orbitrap mass spectrometer was employed for cannabinoid identification and quantification, with standard mixtures prepared in methanol. The extracts yielded significant quantities, such as 6.6%/g from leaves and 3%/g from fruits. Cannabinoids were detected in fruits, leaves, and inflorescences, with acidic forms (CBDA and THCA A) present in higher concentrations than their neutral counterparts. Notably, leaves contained 4.43 × 10−3 µg/g of CBD and 1.05 × 10−3 µg/g of THC. These findings, facilitated by high-resolution analytical methods, underscore the potential of Trema micranthum as an alternative source for cannabinoids, guiding future research in this area.

Geo-electrical investigation for groundwater reserves in the Boranakanive Reservoir Catchment in Tumkur district, Karnataka, India

The groundwater resources have been under significant constraints due to excessive exploitation and climate change. It is imperative to comprehend these dwindling resources' characteristics, given their distribution and occurrence variability across the space and time. A semi-arid climate characterizes the study area and agriculture is the primary occupation, their reliance on groundwater is indispensable due to the lack of surface water sources and erratic rainfall patterns. Consequently, the study employed the Schlumberger array of 200 m-spaced half-current electrodes Vertical Electrical Sounding (VES) survey was conducted at 100 VES locations to identify groundwater potential zones. Further, curve-matching and correlation of lithologies have been carried out from various VES-soundings, and also generated pseudo-cross sections of the subsurface geometry. The results revealed the existence of five geoelectrical resistivity layers as follows layer 1–6 to 422 Ωm, layer 2–8 to 7708 Ωm, layer 3–4 to 37117 Ωm, layer 4–37 to 95059 Ωm, and layer 5–205 to 83142 Ωm. The iso-apparent resistivity maps of the study area reveal values ranging for the first layer 11Ωm - 333 Ωm, the second layer 23Ωm - 5347Ωm, the third layer 11Ωm - 26734Ωm and 10Ωm - 67250Ωm for the fourth layer. The present research determined that the third and fourth layer constitute the principal aquifers due to their fractured structures. As a result, these layers produce a significant quantity of groundwater in the north-eastern (NE) and southern (S) regions of the study region. As a consequence of these findings, further exploration and management of groundwater resources in the study areas can be carried out with valuable insight.

Post-genomic illumination of paclitaxel biosynthesis.

Paclitaxel rapidly became one of the most effective anticancer drugs. However, the production of paclitaxel is hindered by substantial challenges, particularly considering the significant quantities of drug required and the inherently low concentration of paclitaxel and its intermediates in plants. Paclitaxel is currently produced in a so-called semi-synthesis in which baccatin III is extracted from Taxus species and chemically converted to paclitaxel. Despite the fact that many of the intermediates of paclitaxel biosynthesis are yet to be experimentally determined, a set of recent papers-facilitated by the sequencing and assembly of three Taxus genomes-has uncovered the minimal gene sets for both baccatin III and paclitaxel biosynthesis. Here we summarize the key milestones towards our understanding of paclitaxel biosynthesis and highlight recent advancements made possible by genome-level analysis of potential key genes involved. We argue that these studies will ultimately pave the way towards the elucidation of the entire paclitaxel biosynthetic pathway and facilitate the industrial production of paclitaxel via synthetic biology approaches. However, several major challenges lie ahead before we can fully tap into the amazing curative potential that taxanes provide.

Assembly of Dandelion-Like Nanoprobe for Sensitive Detection of N6-Methyladenosine Demethylase by Single-Molecule Counting.

N6-methyladenosine (m6A) demethylase is essential for enzymatically removing methyl groups from m6A modifications and is significantly implicated in the pathogenesis and advancement of various cancers, which makes it a promising biomarker for cancer detection and research. As a proof of concept, we select the fat mass and obesity-associated protein (FTO) as the target m6A demethylase and develop a dandelion-like nanoprobe-based sensing platform by employing biobar-code amplification (BCA) for signal amplification. We construct two meticulously designed three-dimensional structures: reporter-loaded gold nanoparticles (Reporter@Au NPs) and substrate-loaded magnetic microparticles (Substrate@MMPs), which can self-assemble to form dandelion-like nanoprobes via complementary base pairing. In the presence of FTO, the m6A-containing substrates are demethylated, triggering the MazF-assisted cleavage reaction and thereby releasing the Reporter@Au NPs. Furthermore, upon digestion by exonucleases, the Reporter@Au NPs may liberate a significant quantity of Cy3 signals. Remarkably, the combined effects of Au NPs' superior enrichment capacity, MMPs' exceptional magnetic separation efficiency, and the precision of the single-molecule detection platform endow the FTO sensor with exceptional sensitivity and specificity with a detection limit of 7.46 × 10-16 M. Additionally, this method offers a versatile platform for the detection of m6A demethylase and the screening of corresponding inhibitors, thereby advancing clinical diagnosis and drug development.

Genomic profiling and assessment of the plant growth-enhancing traits in the novel actinomycete, Plantactinospora soli sp. nov.

Strain KLBMP 9567T, an isolate from weathered soil, was identified as a new actinobacterial species through a comprehensive polyphasic approach. Phylogenetic evaluations relying on 16S rRNA gene sequence placed KLBMP 9567T within the genus Plantactinospora, alongside its close relatives Plantactinospora veratri NEAU-FHS4T and Plantactinospora sonchi NEAU-QY2T, both sharing 98.6% sequence similarity. However, KLBMP 9567T demonstrated low digital DNA-DNA hybridization values with P. veratri NEAU-FHS4T and P. sonchi NEAU-QY2T, recorded at 48.5 and 29.1%, respectively. Meanwhile, the average nucleotide identity values were correspondingly recorded at 91.1 and 83.1%. The cell wall of KLBMP 9567T contained meso-diaminopimelic acid, with xylose, mannose, glucose and galactose as diagnostic sugars. The diagnostic dominant phospholipids included diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. Given phenotypic and genotypic data, KLBMP 9567T is recognized as a new species within the Plantactinospora genus, named Plantactinospora soli sp. nov., with the type strain KLBMP 9567T (= CGMCC 4.7773T = NBRC 115787T). Genome mining revealed genes associated with plant growth promotion, specifically those encoding enzymes for synthesizing plant hormones like indole acetic acid and gibberellic acid. Experimental validation demonstrated that KLBMP 9567T can produce significant quantities of indole acetic acid (20.6 mg l-1) and gibberellic acid (42.6 mg l-1). Moreover, co-culture with Arabidopsis showed marked growth enhancements: a 56.3% increase in lateral root proliferation, a 16.7% elongation in primary root length and a 44.8% boost in biomass accumulation. These findings underscore the strain's potent plant growth-promoting attributes, providing profound insights into the mechanisms by which KLBMP 9567T enhances plant growth.

Presence of Microplastics in Water, Soil, Organic Fertilizer, and Potato Plants on Potato Plantations

ABSTRACTMicroplastics (MPs) have become a significant environmental concern, but data on their presence in potato gardens are limited. This study aims to identify and quantify MPs in soil, organic fertilizer, irrigation water, and potato plants in potato gardens. Soil, fertilizer, irrigation water, and potato samples were collected from potato fields. Analysis was carried out using a microscope and Fourier transform infrared spectroscopy (FTIR) to identify the shape, color, quantity, and type of MP polymer. The results indicate significant MPs contamination with the most dominant shape of fibers (86%) and fragments (8%), dominant color black (35%) and blue (27%), abundances of MPs 0.10–9.20 g−1, as well as the presence of polyethylene terephthalate (PET) and high‐density polyethylene (HDPE) polymers. These MPs have the potential to harm plant growth and soil quality. MPs are found in significant quantities in potato gardens and have the potential to disrupt agricultural ecosystems. The presence of MPs in potato gardens can affect the quality of agricultural products and human health throughout the food chain.