Significant Increase In Fresh Weight Research Articles

Impact of Seaweed Extract, Chelated Manganese and Some Chemical Substances on Growth Parameters and Yield Components of Cauliflower (Brassica oleracea var, botrytis)

The study were conducted to investigate the influence of seaweed extract, chelated manganese, and two other chemical substances that are known as Agro promotor1(substance No.48) and Agro promotor2 (substance No.50) on the growth, yield, and quality of cauliflower. Results revealed that yield and its components increased with using each seaweed extract(3ml/l), chelated manganese (12%) at a concentration of 0.25g/l, and Agro promotor1 (substance No.48) at a concentration of 3 ml/l compared with control (untreated) treatment. Using both seaweed and chelated manganese treatments exceeded other foliar spraying treatments and caused a significant increase in fresh weight of the whole plant, leaves fresh weight,leaves dry weight percent, yield, TSS and vitamin C. The increase in total yield ranged from 12.8-48.2% by using the previous treatments as compared with control. Also, foliar sprays of seaweed extracts led to the earliness in cauliflower production as compared with the control. This study recommends using seaweed extract at a concentration of 3ml/l or chelated manganese (12%) at a concentration of (0.25g/l) for cauliflower production. Agro promotor1 (substance No.48) at a concentration of 3 ml/l can be applied in case of unavailability of seaweed extract or chelated manganese (12%).

Diffusions of sound frequencies designed to target dehydrins induce hydric stress tolerance in Pisum sativum seedings

Among plant responses to environmentally induced stress modulating protein expression appears to be a key stage in inducible signaling. Our study was focused on an innovative strategy to stimulate plant stress resistance, namely, the use of targeted sequences of specific sound frequencies.The influence of acoustic stimulation on plant protein synthesis was investigated. In our study green peas, Pisum sativum, were cultured under hydric stress conditions with targeted acoustic stimulation. Acoustic sequences targeting dehydrins (DHN) which accumulate in plants in response to dehydration were studied. We experimented on pea seeding with two different sequences of sounds: the first one corresponded to DHN cognate protein and the second one was aimed at the DHN consensus sequence. Shoot elongation after pea seed germination was estimated by fresh weight gain studied in the presence of various conditions of exposure to both sequences of sounds. DHN expression in peas was quantified via ELISA tests and Western-blotting by using specific antibodies.A significant increase in fresh weight in peas grown under exposure to the DHN cognate sound sequence was observed, whereas the consensus sound sequence had no effect on growth. Moreover, the 37kDa DHN amount was increased in peas treated with the consensus acoustic sequence. These results suggest that the expression of DHN could be specifically modulated by a designed acoustic stimulus.

Gamma irradiation induced effects on in vitro shoot cultures and influence of monochromatic light regimes on irradiated shoot cultures of Dendrobium sonia orchid

The effects of gamma radiation and monochromatic lights on growth of in vitro shoot cultures of orchid, Dendrobium sonia, were investigated. The gamma irradiated shoot cultures grown under white, blue, yellow and red monochromatic lights exhibited differential growth pattern. Shoot cultures gamma irradiated at 15–45 Gy showed reduced shoot length, fresh weight and leaf area. The monochromatic light significantly influenced survival rate and growth of irradiated shoots. The yellow and red light treatments positively influenced survival of gamma irradiated shoots with significant increase in fresh weight, shoot length and chlorophyll content. Yellow light was found to be most effective as leaf area was increased across the radiation dose range (15–100 Gy) compared to red light. The results demonstrated that the method of post-irradiation exposure could be useful to improve growth of gamma irradiated in vitro shoots, and help to recover orchid mutants with novel modifications.

Actinobacteria Associated With Arbuscular Mycorrhizal Funneliformis mosseae Spores, Taxonomic Characterization and Their Beneficial Traits to Plants: Evidence Obtained From Mung Bean (Vigna radiata) and Thai Jasmine Rice (Oryza sativa).

In this study, we report on the isolation of actinobacteria obtained from spores of Funneliformis mosseae and provide evidence for their potential in agricultural uses as plant growth promoters in vitro and in vivo. Actinobacteria were isolated from spores of F. mosseae using the dilution plate technique and media designed for the selective isolation of members of specific actinobacterial taxa. Six strains namely 48, S1, S3, S4, S4-1 and SP, were isolated and identified based on16S rRNA gene sequences. Phylogenetic analysis showed that isolate SP belonged to the genus Pseudonocardia with P. nantongensis KLBMP 1282T as its closest neighbor. The remaining isolates belonged to the genus Streptomyces. Two isolates, 48 and S3 were most closely related to S. thermocarboxydus DSM 44293T. Isolates S4 and S4-1 shared the highest 16S RNA gene similarity with S. pilosus NBRC 127772T. Isolate S1 showed its closest relationship with the type strain of S. spinoverrucosus NBRC14228T. The ability of these isolates to produce indole-3-acetic acid (IAA), siderophores and the ability to solubilize phosphate in vitro were examined. All isolates produced siderophores, four isolates produced IAA and two isolates solubilized inorganic phosphate at varying levels. S. thermocarboxydus isolate S3 showed the highest IAA production with high activities of phosphate solubilization and siderophore production. The inoculation of mung beans (Vigna radiata) with this strain resulted in a significant increase in fresh weight, root length and total length as an effect of IAA production. In an experiment with rice (Oryza sativa), S. thermocarboxydus isolate S3 promoted the growth of rice plants grown in low nutritional soil under induced drought stress. This report supports the view that the inoculation of rice with plant growth promoting actinobacteria mitigates some adverse effects of low nutrient and drought stress on rice.

Expression Profiling of Ribosomal Protein Gene Family in Dehydration Stress Responses and Characterization of Transgenic Rice Plants Overexpressing RPL23A for Water-Use Efficiency and Tolerance to Drought and Salt Stresses.

Our previous findings on the screening of a large-pool of activation tagged rice plants grown under limited water conditions revealed the activation of Ribosomal Protein Large (RPL) subunit genes, RPL6 and RPL23A in two mutants that exhibited high water-use efficiency (WUE) with the genes getting activated by the integrated 4x enhancers (Moin et al., 2016a). In continuation of these findings, we have comprehensively characterized the Ribosomal Protein (RP) gene family including both small (RPS) and large (RPL) subunits, which have been identified to be encoded by at least 70 representative genes; RP-genes exist as multiple expressed copies with high nucleotide and amino acid sequence similarity. The differential expression of all the representative genes in rice was performed under limited water and drought conditions at progressive time intervals in the present study. More than 50% of the RP genes were upregulated in both shoot and root tissues. Some of them exhibited an overlap in upregulation under both the treatments indicating that they might have a common role in inducing tolerance under limited water and drought conditions. Among the genes that became significantly upregulated in both the tissues and under both the treatments are RPL6, 7, 23A, 24, and 31 and RPS4, 10 and 18a. To further validate the role of RP genes in WUE and inducing tolerance to other stresses, we have raised transgenic plants overexpressing RPL23A in rice. The high expression lines of RPL23A exhibited low Δ13C, increased quantum efficiency along with suitable growth and yield parameters with respect to negative control under the conditions of limited water availability. The constitutive expression of RPL23A was also associated with transcriptional upregulation of many other RPL and RPS genes. The seedlings of RPL23A high expression lines also showed a significant increase in fresh weight, root length, proline and chlorophyll contents under simulated drought and salt stresses. Taken together, our findings provide a secure basis for the RPL gene family expression as a potential resource for exploring abiotic stress tolerant properties in rice.

Rhizobacteria of Populus euphratica Promoting Plant Growth Against Heavy Metals

The heavy metal-resistant bacteria from rhizospheric soils of wild Populus euphratica forest growing in arid and saline area of northwestern China were investigated by cultivation-dependent methods. After screening on medium sparked with zinc, copper, nickel and lead, 146 bacteria strains with different morphology were isolated and most of them were found to be resistant to at least two kinds of heavy metals. Significant increase in fresh weight and leaf surface area of Arabidopsis thaliana seedlings under metal stress were noticed after inoculated with strains especially those having multiple-resistance to heavy metals such as Phyllobacterium sp. strain C65. Investigation on relationship between auxin production and exogenous zinc concentration revealed that Phyllobacterium sp. strain C65 produced auxin, and production decreased as the concentration of zinc in medium increased. For wheat seedlings treated with zinc of 2 mM, zinc contents in roots of inoculated plants decreased by 27% (P < 0.05) compared to the uninoculated control. Meanwhile, zinc accumulation in the above-ground tissues increased by 22% (P < 0.05). The translocation of zinc from root to above-ground tissues induced by Phyllobacterium sp. strain C65 helped host plants extract zinc from contaminated environments more efficiently thus alleviated the growth inhibition caused by heavy metals.

Organ-distinctive changes in respiration rates of rice plants under nickel stress

Nickel (Ni) is an essential mineral element that may accumulate to toxic levels in soils due to anthropogenic activities. The growth of rice plants cultured hydroponically was severely impaired when Ni concentration was raised from 0.1 to 0.5 mM. However, the decrease in plant growth was not accompanied by any significant effect on respiration rates at the whole plant level. Short-term treatments of excised roots with 0.1–1.0 mM Ni did not result in any significant changes in respiration. However, long-term treatments of whole plants induced clear distinctive effects on shoots and roots. There was a significant decline in growth of these organs when the plants were cultured for 10 days at 0.5 mM Ni. Although no significant changes in respiration occurred in shoots, it was drastically reduced in roots. Ni withdrawal after 5 days’ treatment caused an increase in respiration in roots, but a decrease in shoots, despite the fact that stress alleviation induced a similar and significant increase in fresh weight of both organs. Our results point to organ-related differences in the distribution of the energy resulting from respiration under Ni stress.

(67) Effect of Two Types of Fertilizers on the Growth and Development of Tissue-cultured Daylilies Transferred to the Greenhouse

During Summer 2005, a study similar to that of Summer 2004 was conducted to determine the effect of two type of fertilizers on the growth and development of tissue-cultured daylilies transferred to the greenhouse. Peters 20–20–20 water-soluble fertilizer and a slow-release fertilizer were the two fertilizers evaluated. Peters 20–20–20 fertilizer was used at 0 (control), 50, 100, 200 mg/L rates. The slow-release fertilizer was used at 2.5 g per 10.2-cm pot. Each treatment was replicated four times in randomized complete-block design. After 6 weeks of growth, the results showed that when compared to the control, all treatments except for 200 mg/L caused a significant increase in root growth. Shoot growth was significantly increased by the 100 mg/L treatment, while the 200 mg/L and the slow-release treatments suppressed shoot growth. Similar to the growth of roots and shoots, the 100 mg/L treatment caused significant increase in fresh weight, while both the 200 mg/L and slow-release treatments caused a reduction. Results obtained for Summer 2005 were similar to that of Summer 2004. These results imply that the 100 mg/L Peters 20–20–20 fertilizers treatment is the best treatment for maximum growth and development of tissue-cultured daylilies transferred to the greenhouse.

EFFECT OF INOCULATION TIMING WITH ARBUSCULAR MYCORRHIZAL FUNGI ON GROWTH AND FLOWERING OF MICROPROPAGATED Chrysanthemum morifolium

A pot experiment was carried out to evaluate the effect of arbuscular mycorrhizal fungi (AMF) inoculation timing on growth and flowering of Chrysanthemum morifolium cuttings. AMF inocula were either directly applied to cutting (AMFC), or applied at transplanting stage (AMFT). The data showed: a significant difference in plant growth of AMF treatment compared with non-inoculated treatment at transplanting stage. Rooting rate in AMF treatment was 99% whereas it was 77% in non-mycorrhizal inoculated. The colonization rate was 53.9% in AMF treatment, while no in non-AMF treatment. Tap root length and number of lateral roots in AMF treatments were twice of those recorded for non-AMF treatments. Inoculation of AMF significantly increased shoot and root growth at transplanting stage. After transplantation, chrysanthemum plants in AMFC and AMFT treatments had 76.42 and 64.24% colonization rate, respectively. Plant height, leaf area, root length, fresh and dry weight of shoots, stems and roots in AMF inoculation treatments (AMFC and AMFT) increased significantly than those of control plants. AMF inoculation significantly shortened flowering time compared with non-AMF plants. Fresh weight, width and length of flowers in AMFC and AMFT treatments were generally higher than those in control. However, a significant increase in fresh weight, width and length of flowers was found in AMFC compared with AMFT treatment. A significant increase of macronutrient concentrations in leaves was observed for AMFC treatment compared with control. Mn concentration in AMFC and AMFT was more than double of that in control. In roots, macro and micronutrient concentrations were generally higher in AMFC than AMFT or control treatments

(133) Influence of Two Types of Fertilizers on the Growth and Development of Tissue-cultured Daylilies Transferred to the Greenhouse

During Summer 2004, a study was conducted to determine the effect of two types of fertilizers on the growth and development of tissue-cultured daylilies transferred to the greenhouse. Peters 20–20–20 water-soluble fertilizer and a slow-release fertilizer were the two fertilizers evaluated. Peters 20–20–20 fertilizer was used at 0 (control), 50, 100, and 200 mg·L–1 rates. The slow release fertilizer was used at 2.5 g per 10.2 cm pot. Each treatment was replicated four times in a randomized complete-block design. After 6 weeks of growth, the results showed that, when compared to the control, all treatments except for 200 mg·L–1 caused a significant increase in root growth. Shoot growth was significantly increased by the 100 mg·L–1 treatment, while the 200 mg·L–1 and slow-release treatments suppressed shoot growth. Similar to root and shoot growth, the 100 mg·L–1 treatment caused a significant increase in fresh weight, while the 200 mg·L–1 and slow-release treatments caused a reduction. These results imply that the 100 mg·L–1 Peters 20–20–20 fertilizer treatment is the best treatment for maximum growth and development of tissue-cultured daylilies transferred to the greenhouse.

Spray Application of Electrolyzed Acidic and Alkaline Water on Leeks for Reduction of Viable Bacteria and Growth Promotion

An alternate weekly spray application of electrolyzed acidic water and alkaline water on leaf surfaces of leeks in solution culture reduced bacterial counts at harvest by about 1.0 log for total viable bacteria and about 1.5 log for coliform group as compared with the control lot using the conventional cultivation method. In the examination of the growth conditions at harvest, the alternate spray lot showed significant increase in fresh weight without any acidic burns. These results suggested that the alternate weekly spray application of acidic water and alkaline water on leaf surfaces in solution culture is an effective treatment for both microbial safety and growth promotion in agricultural production.

Glutathione-induced growth of embryogenic tissue of white spruce correlates with changes in pyrimidine nucleotide metabolism

Exogenous applications of reduced glutathione (GSH) and oxidized glutathione (GSSG) promote growth of embryogenic tissue of white spruce during a 7-day subculture period. Compared to control tissue, a statistically significant increase in fresh weight, as well as RNA and DNA content, was observed in the presence of GSH and GSSG during the last days in culture. The effects of these two metabolites on pyrimidine nucleotide metabolism was investigated by following the metabolic fate of 14C-orotic acid, a precursor of the de novo synthesis, and 14C-uridine and 14C-uracil, intermediates of the respective salvage and degradation pathways. Compared to control embryos, GSH-treated tissue was able to utilize a larger fraction of supplied orotic acid for UMP production, possibly due to the increased activity of orotate phosphoribosyltransferase (OPRT). The activity of this enzyme increased markedly in tissue cultured with GSH during the last days in culture. Salvage of uridine for nucleotide and nucleic acid synthesis was observed in all treatments, especially in GSSG-treated tissue at day 7. The increased salvage activity in this tissue correlated with the increase in activities of the two uridine salvage enzymes, uridine kinase (URK) and nucleoside phosphotransferase measured with uridine (NPT(uridine)). Compared to control tissue, tissue treated with either GSH or GSSG was able to utilize a large fraction of uracil for nucleotide synthesis, denoting a better ability to divert this precursor from degradation. Nucleotide and nucleic acid analyses revealed that in both GSH and GSSG-treated tissue, the endogenous levels of UTP, CTP, as well as those of RNA and DNA, were increased compared to those of control tissue. Overall, the results from this study suggest that GSH and GSSG can induce growth of embryogenic tissue of white spruce through distinct metabolic changes of pyrimidine nucleotides.

Effect of the different timing of AMF inoculation on plant growth and flower quality of chrysanthemum

Plant growth and flower quality of an ornamental plant ( Chrysanthemum morifolium Ramat) var. Baekgwang in response to the different timing of arbuscular mycorrhizal fungi (AMF) inoculation were examined. To evaluate the effects of AMF inoculation timing on growth of chrysanthemum cuttings, AMF was directly inoculated to cutting (AMFD), or inoculated at transplanting stage (AMFA). Control plants did not receive AMF inoculation (CON). A significant difference in plant growth was observed in AMF treatment compared to non-inoculated treatment at transplanting stage. Rooting rate in AMF treatment was 99% whereas it was 76% in non-mycorrhizal inoculation. The colonization rate was 3.9% in AMF treatment, while it was 0% in non-AMF treatment. Tap root length and number of lateral roots in AMF treatment were twice than those of non-AMF treatment. Inoculation of AMF significantly increased shoot and root growth at transplanting stage. At 7 weeks after transplanting, chrysanthemum plants in AMFD and AMFA treatments had 46.42 and 34.24% colonization rate, respectively. Low colonization rate (6.08%) was observed in CON, possibly due to contamination. Plant height, leaf area, root length, and fresh and dry weight of shoots, stems and roots in AMF inoculation treatments (AMFD and AMFA) were significantly higher than control plants. AMF inoculation significantly shortened flowering time compared to non-AMF plants. Chrysanthemum in AMFD, AMFA and CON treatments flowered at 98, 104 and 112 days after transplanting, respectively. Fresh weight, width and height of flowers in AMFD and AMFA treatments were generally higher than those in CON. However, a significant increase in fresh weight, width and height of flowers was found in AMFD treatment. A significant increase of macronutrient uptake in leaves was observed for AMFD treatment, compared with CON. Mn uptake in AMFD and AMFA was more than double than that in CON. In roots, macro and micronutrient concentrations were generally higher in AMFD.

Interaction of root-knot nematode and mycorrhizal fungi on common beanPhaseolus vulgaris L.

The interactions of root-knot nematode and VAM fungus on common bean plants (Phaseolus vulgaris L.), and the sequence of nematode infection were studied in the greenhouse. The inoculation with VAM fungus caused a significant increase in plant height and fresh weight compared with non treated plants.Meloidogyne infection unsignificantly decreased plant height and dry weight. When fungus was inoculated at 15 and 30 days prior toM. incognita infection, a significant increase in fresh weight was observed. There were no significant differences in total nitrogen (mg/plant) between plants inoculated withM. incognita alone or those inoculated with bothM. incognita and VAM fungus at the same time or 15 days after the fungus inoculation. Plants preinoculated with VAM fungus 30 days prior to nematode infection had a significant increase in total nitrogen. The inoculation with VAM fungus caused a significant increase in phosphorus content. However, it was significantly decreased in plants inoculated with nematode alone and in plants inoculated with VAM fungus andM. incognita at the same time. Gall index and final nematode population were significantly increased when nematodes were treated at the same time with fungus, although there were significant decrease in nematode final population and gall index when the plants were treated with nematodes at 15 and 30 days after mycorrhizal infection. A decrease in percentage of fungal colonization was observed when nematodes were inoculated with fungus at the same time.