Significant Differences In Iron Content Research Articles

Dissolved Domoic Acid Does Not Improve Growth Rates and Iron Content in Iron-Stressed Pseudo-Nitzschia subcurvata

Many regions of Antarctica are classified as high nutrient low chlorophyll (HNLC) areas. In these, iron availability is limiting primary productivity and subsequent carbon export. Domoic acid (DA) has previously been detected in the Southern Ocean and suggested to act as a ligand that facilitates iron assimilation for Pseudo-nitzschia spp., species that contribute to Antarctic diatom blooms. An incubation experiment using the Antarctic species Pseudo-nitzschia subcurvata was performed in Antarctic seawater at low and high iron concentrations. Dissolved DA was added to one set of each of the two treatments. This was done to verify whether DA positively affects the growth of the non-toxic species Pseudo-nitzschia subcurvata and increases its cellular iron content, particularly under low iron conditions. We hypothesize that (i) DA is taken up under low iron conditions (ii) that more iron is taken up if DA is available and (iii) that the growth rate increases in the presence of DA. We showed that P. subcurvata did not take up any added DA, even under low iron conditions. Additionally and contrary to our hypothesis, the cells were not positively influenced by the addition of dDA in terms of growth rate, cellular iron and carbon content. Hence, there was no significant difference in iron content between the different treatments. However, the cellular copper content increased under low iron conditions when DA was added. This study suggests that dissolved DA in naturally occurring concentrations does not increase bioavailability of iron to P. subcurvata and that only species producing DA might benefit from it.

Combined use of fluorescence with a magnetic tracer and dilution effect upon sentinel node localization in a murine model.

BackgroundSentinel node biopsy using radioisotope and blue dye remains a gold standard for axillary staging in breast cancer patients with low axillary burden. However, limitations in the use of radioisotopes have resulted in emergence of novel techniques. This is the first in vivo study to assess the feasibility of combining the two most common novel techniques of using a magnetic tracer and indocyanine green (ICG) fluorescence.Materials and methodsA total of 48 mice were divided into eight groups. Groups 1 and 2, the co-localization groups, received an injection of magnetic tracers (Resovist® and Sienna+®, respectively) and ICG fluorescence; distilled water was used as the solvent of ICG. Groups 3 and 4, the diluted injection groups, received an injection of magnetic tracers (Resovist and Sienna+, respectively) and saline for dilution. Groups 5, 6, and 7, the control groups, received magnetic tracer (Resovist, Sienna+) and ICG alone, respectively. Fluorescent intensity assessment and iron quantification of excised popliteal lymph nodes were performed. Group 1′, a co-localization group, received an injection of magnetic tracers (Resovist) and ICG′ fluorescence: saline was used as the solvent for ICG.ResultsLymphatic uptake of all tracers was confined to the popliteal nodes only, with co-localization confirmed in all cases and no significant difference in fluorescent intensity or iron content of ex vivo nodes between the groups (except for Group 1′). There was no impact of dilution on the iron content in the diluted Sienna+ group, but it significantly enhanced Resovist uptake (P=0.005). In addition, there was a significant difference in iron content (P=0.003) in Group 1′.ConclusionThe combination of a magnetic tracer (Resovist or Sienna+) and ICG fluorescence is feasible for sentinel node biopsy and will potentially allow for precise transcutaneous node identification, in addition to accurate intraoperative assessment. This radioisotope-free “combined technique” warrants further assessment within a clinical trial.

The effect of PGPR and alfalfa extract on macronutrient and micronutrient contents of sorghum (Sorghum vulgare)

ABSTRACT- The increasing population and the pastures inability to meet food needs in livestock have led to the increase of cultivates forage plants. In order to evaluate the effect of plant growth promoting rhizobacteria (PGPR) and alfalfa (Medicago sativa) extract on the growth and concentration of macronutrient and micronutrient contents of forage sorghum (Sorghum vulgare), an experiment was conducted as factorial aaraned in a completely randomized design with six replications at Research Greenhouse University of Zabol. The first factor was PGPR (included soil inoculated and non-inoculated (control) medium, and the second factor was alfalfa extract in three concentrations (0, 2 and 4 ppm). The results showed that increasing alfalfa extract concentration significantly increased total plant dry weight as well as the macro and micronutrient contents. The highest and lowest manganese contents were achieved in the 0.004 levels and control of alfalfa extracts, respectively. Copper content in 0.002 level of alfalfa extract showed a 15% increase compared to the control, with no significant difference with 0.004 of alfalfa extracts. PGPR application as growing substrate also significantly increased dry weight as well as manganese, copper and potassium contents of the plant. Also, there was no significant difference in iron content at level 4 per thousand of alfalfa extract in a substrate inoculated with PGPR compared to the non-inoculated substrate. Finally, it is recommended that the effect of alfalfa extract on yield and nutrient concentrations of other plants be examined in the future.

Insights into the role of iron in immature rat model of hypoxic-ischemic brain injury.

This study aimed to investigate the role of iron in the occurrence and development of hypoxic-ischemic brain injury (HIBI) in immature rat models using 3-day-old Sprague Dawley rats. Normal control (NC), hypoxic-ischemic (HI), anemia, HI + ischemia, early iron treatment and late iron treatment groups were established. Rat brain tissue sections were stained with hematoxylin and eosin and pathologically evaluated. Iron content and mRNA expression levels of iron regulatory protein 2 (IRP2) and transferrin receptor in the brain tissues were measured. Ultrastructural changes in the actin, microtubules, myelin and mitochondria of oligodendrocytes and axons were examined by electron microscopy. Numbers of viable myelin sheaths and oligodendrocytes in the periventricular area were also observed. Pathological damage of brain tissue in the HI group was markedly increased compared with that in the NC group. Furthermore, there was a higher iron content and reduced number of viable oligodendrocytes in the periventricular area of the HI group compared with the NC group. No significant difference in iron content was observed between the HI + anemia and NC groups. The number of viable oligodendrocytes in the HI + anemia group was increased compared with that in the HI group, and the number in the HI + anemia group with late iron treatment was lower compared with that in the NC group and increased compared with that in the HI + anemia group. Electron microscopy revealed a significantly higher number of myelin sheaths in the HI + anemia group than in the HI group. IRP2 mRNA expression levels in the brain tissues were significantly decreased in the HI + anemia group compared with the HI group. The results suggest that anemia may reduce the rate of increase of iron content of the brain following HI. However, the early occurrence of anemia may protect against HIBI.

I01 Multimodal MRI assessment: a biomarker of presymptomatic Huntington's disease

BackgroundNeuroimaging studies showed impairment of the basal ganglia and thalamus in preclinical stages of Huntington's disease (HD) characterised by progressive volume loss, microstructural impairment and microglial activation. Therefore, biomarkers sensitive...

Effects of Organic Iron Supplementation on the Performance and Iron Content in the Egg Yolk of Laying Hens

An experiment was conducted to determine the efficacy of dietary iron-soy proteinate (Fe-SP) and iron-methionine chelate (Fe-Met) on the performance of laying hens and iron content in egg yolk. Eight hundred Hy-Line Brown laying hens of 68wk old were housed in 400 cages of 2 birds each. Two hundred birds (10 cages×10 replicates) were assigned to one of the following four treatments: Control; non supplementation with Fe-SP or Fe-Met, Fe-Met 100; 100ppm iron supplementation with Fe-Met, Fe-SP 100; 100ppm iron supplementation with Fe-SP, Fe-SP 200; 200ppm iron supplementation with Fe-SP. Results of 35d feeding trial showed that there were significant differences in egg production, egg weight, feed conversion ratio and Haugh unit among the treatments. Egg weight and Haugh unit of Fe-SP 200 were significantly higher than the control. Hen-day egg production and feed conversion ratio of Fe-SP 100 and Fe-SP 200 were not significantly different from those of the control. Eggshell color was significantly improved in the Fe supplementation treatments compared to the control. The iron content of egg-yolk was maximized 5wk after feeding supplemental Fe and that of Fe-SP 100 was highest being 16.6% higher than the control. There were no significant differences in iron content of egg-yolk between source and level of iron supplementation. Copper content in the egg-yolk was not significantly affected by treatments but zinc content was significantly increased in iron supplemented treatments at 5th week after feeding. In conclusion, Fe content of egg-yolk could be effectively increased by supplementing 100ppm iron as iron-soy proteinate for 5wks. No significant difference was found in Fe content of egg yolk between Fe-SP and Fe-Met.

The Effect of Dietary Supplementation of Fe-methionine Chelate and FeSO4 on the Iron Content of Broiler Meat

A broiler experiment was conducted to compare the effects of supplementary iron sources and levels on the iron meat Two hundred and fifty hatched Ross broiler chickens were randomly assigned to 5 dietary treatments. Each coment ui oroner meat. two nundred ana arty naicnca Ross oroner chickens were randomly assignea to 3 Dietary treatments. nacn treatment had 5 replicates of 10 birds (5 males and 5 females). Birds were housed in raised floor batteries and fed traditional broiler diets ad libitum for 5 weeks. Dietary treatments were as follows: Control, Fe-Met 100 (100 ppm iron as Fe-methionine), Fe-Met 200, FeSO 4 100 (100 ppm iron as FeSO 4 ·7H 2 O) and FeSO 4 200. There were no significant differences among treatments in parameters related to production performance. Liver contained approximately 10 times more iron than the leg muscle which contained approximately 3 times more iron than either breast muscle or wing muscle. Significant differences in iron content in the broiler meat were observed. In the breast meat, Fe-Met treatments were significantly (p<0.05) higher than other treatments in iron content. In the leg meat, Fe-Met treatments and FeSO 4 200 treatment were significantly higher than the control in iron content. In the wing muscle, Fe-Met 200 treatment was significantly higher than other treatments in iron content. Iron content in the liver was significantly influenced by source and supplementation level of iron. Fe-Met treatments were higher than FeSO 4 treatments and 200 ppm treatments were higher than 100 ppm treatments in iron content in the liver. It is concluded that iron-methionine chelate is more efficient than iron sulfate and 200 ppm iron supplementation as Fe-Met is recommended for maximum iron enrichment in broiler meat.