Significant Difference In Survival Rate Research Articles

Effects of fishmeal substitution by α-galactosidase hydrolytic soybean meal (EhSBM) on growth, antioxidant capacity, inflammatory responses and intestinal health of turbot juveniles (Scophthalmus maximus L.)

An 8-week feeding trial was conducted to study the effects of fishmeal substitution by different levels of α-galactosidase hydrolytic soybean meal (EhSBM) on growth, antioxidant capacity, inflammatory responses and intestinal health of turbot juveniles (Scophthalmus maximus L.). Four isonitrogenous (47.63% crude protein) and isolipidic (10.87% crude lipid) diets were formulated. The FM diet (the control diet) was formulated with 55% fishmeal. On basis of the FM diet, three experimental diets (Diets EhSBM40, EhSBM45 and EhSBM50) were formulated with 40%, 45% and 50% fishmeal protein substitution by EhSBM protein, respectively. Each diet was randomly allocated to triplicate groups in fiberglass tanks (200L), and each tank was stocked with 30 fish (initial weight 4.87 ± 0.01 g). Results showed that there were no significant differences in survival rate, feed intake and feed efficiency among dietary treatments (P > 0.05). The specific growth rate (SGR) of turbot juveniles showed a significantly linear decrease with increasing dietary EhSBM (P < 0.05), while no significant difference was found in fish fed diets control and EhSBM40 (P > 0.05). With increasing dietary EhSBM, the apparent digestibility coefficient of dry matter (ADCD) and protein (ADCP) exhibited significantly quadratic and linear decline, respectively (P < 0.05). When replacing EhSBM protein >40%, the ADCD and ADCP were significantly lower than those of the control group (P < 0.05). The foregut trypsin and α-amylase activity exhibited significantly linear decrease with increasing dietary EhSBM (P < 0.05). Fish fed diets EhSBM45 and EhSBM50 exhibited significantly lower trypsin and α-amylase activity compared with those of the control group (P < 0.05). Moreover, the liver antioxidant capacity showed a significantly linear decrease with increasing dietary EhSBM (P < 0.05), and there was a significantly decrease in fish fed the diet EhSBM50 than those of the control and EhSBM40 groups (P < 0.05). The serum transaminase activity exhibited significantly linear and quadratic increase with increasing dietary EhSBM (P < 0.05). The pro-inflammatory factor mRNA expressions of liver and hindgut were significantly linear up-regulated with increasing dietary EhSBM, and significant differences were observed in fish fed the diet EhSBM50 than those of the control group (P < 0.05). Furthermore, the hindgut morphology index showed significantly linear and quadratic decrease with increasing dietary EhSBM (P < 0.05). The mRNA expressions of hindgut tight junction protein were significantly linear down-regulated with increasing dietary EhSBM (P < 0.05), but no significant difference was found in fish fed the control and EhSBM40 diets (P > 0.05). Meanwhile, there was a significant increase in the relative abundance of Lactobacillaceae and Clostridium butyricum, and a significant decrease of Vibrio and Pseudomonas of hindgut in fish fed three EhSBM diets than those of the control group (P < 0.05). In conclusion, 40% of fishmeal protein could be replaced by EhSBM protein without significantly reducing growth, antioxidant capacity, intestinal health and inducing inflammatory responses. Meanwhile, intestinal microorganism components were significantly improved in fish fed three EhSBM diets.

Development of a 4-miRNA prognostic signature for endometrial cancer.

To develop an effective uterine corpus endometrial carcinoma (UCEC) risk assessment tool to monitor treatment outcomes. Limma package was used to analyze differentially expressed microRNAs (miRNAs) between UCEC tissues and normal tissues in the TCGA database. According to univariate Cox risk regression, least absolute shrinkage, and selection operator (LASSO) Cox analysis were performed to screen prognostic miRNAs and construct a risk scoring model. The prognostic performance of signature was evaluated by Kaplan–Meier and receiver operating characteristic. Multivariate Cox regression analysis was used to determine the independent prognostic factors of UCEC. Nomogram was constructed according to age, clinical stage, and risk score. A 4-miRNA signature based on miR-31-5p, miR-34a-5p, miR-26a-1-3p and miR-4772-3p was established. Risk scores of each patient were calculated by the 4-miRNA signature. After z-score, the patients were divided into high- and low-risk groups. The overall survival of high-risk patients was significantly shorter than that of low-risk patients, pointing to the high performance and independence of the 4-miRNA signature in predicting UCEC prognosis. The nomogram showed a high accuracy in predicting overall survival of UCEC patients. We developed a 4-miRNA signature that could effectively predict the prognosis of UCEC.

Supplementing artemisinin positively influences growth, antioxidant capacity, immune response, gut health and disease resistance against Vibrio parahaemolyticus in Litopenaeus vannamei fed cottonseed protein concentrate meal diets

Artemisinin (ART) is a kind of Chinese herbal medicine worth exploring, which obtains various physiological activities. In order to study the prebiotic effect of ART on Litopenaeus vannamei fed cottonseed protein concentrate meal diets, six groups of isonitrogenous and isolipid diets were prepared (including the fish meal control group, FM; cottonseed protein concentrate replacing 30% fishmeal protein and supplementing ART groups: ART0, ART0.3, ART0.6, ART0.9, and ART1.2). The feeding trials was lasted for 56 days. The results showed that the final body weight, weight gain and specific growth rate of the ART0.6 group were the highest, yet the feed coefficient rate of the ART0.6 group was the lowest significantly (P < 0.05). There was no significant difference in survival rate among treatments (P > 0.05). In serum, the content of malondialdehyde in ART0 group was the highest (P < 0.05); the activities of superoxide dismutase, catalase, phenol oxidase and lysozyme increased firstly and then decreased among the ARTs groups (P < 0.05). The activities of intestinal digestive enzymes (including the trypsin, lipase and amylase) showed an upward trend among the ARTs groups (P < 0.05). The histological sections showed that the intestinal muscle thickness, fold height and fold width in the FM group were significantly better than those in the ART0 group; while the mentioned above morphological indexes in the ART0 group were significantly lowest among the ARTs groups (P < 0.05). Sequencing of intestinal microbiota suggested that the microbial richness indexes firstly increased and then decreased (P < 0.05); the bacterial community structure of each treatment group was almost close; the relative abundance of pathogenic bacteria decreased significantly (P < 0.05), such as the Proteobacteria and Cyanobacteria at phylum level, besides the Vibrio and Candidatus Bacilloplasma at genus level. In intestinal tissue, the relative expression levels of TOLL1, TRAF6 and Pehaeidih3 showed up-regulated trends, while the expression of Crustin and LZM firstly up-regulated and then down-regulated (P < 0.05). The challenge experiment suggested that the cumulative mortality of FM group was significantly lower than that of ART0 group; besides the cumulative mortality firstly increased and then decreased between the ARTs groups (P < 0.05). In conclusion, the dietary supplementation of ART can improve the growth, antioxidant capacity, immune response, gut health and disease resistance of the shrimp. To be considered as a dietary immune enhancer, the recommended supplementation level of ART in shrimp's cottonseed protein concentrate meal diets is 0.43%.

Decomposition abilities and characteristics of pig manure using three insect larvae

AbstractThis study investigated the effects of three insect larvae on decomposition abilities and survival rates in Experiment 1 or on pH, total nitrogen, total carbon, and C/N ratio in Experiment 2. A total of hatched 7,500 Tenebrio molitor (TM) larvae, hatched 501 Protaetia brevitarsis seulensis (PBS) larvae, and hatched 15,000 Ptecticus tenebrifer (PT) larvae were randomly subjected to three treatments with three replicates. The treatments were as follows: (1) TM, 5,000 g pig manure + 250 g TM larvae; (2) PBS, 5,000 g pig manure + 250 g PBS larvae; and (3) PT, 5,000 g pig manure + 250 g PT larvae. In Experiment 1, there were significant differences (p &lt; 0.05) in the decomposition abilities of the pig manure from the three insect larvae‐treated groups for 15–30 days. No significant differences in survival rates were observed for any of the in all treatments over time. Decomposition ability and survival rates in pig manure were higher in the PT larvae group than in the PBS and PT larvae groups. In Experiment 2, three insect larvae‐treated pig manure treatments had an effect on total nitrogen for 20 and 30 days, total carbon for 10 and 30 days, and C/N ratio for 20 and 30 days. Thus, our results suggest that the three insect larvae have the ability to decompose pig manure to increase for increasing survival rates or might represent a valuable alternative as organic fertilizer, which could increase the total nitrogen content and decrease C/N ratio.

Study on the antagonistic effects of koumiss on Toxoplasma gondii infection in mice.

Toxoplasma gondii is an important food-borne zoonotic parasite, and approximately one-third of people worldwide are positive for T. gondii antibodies. To date, there are no specific drugs or vaccines against T. gondii. Therefore, developing a new safe and effective method has become a new trend in treating toxoplasmosis. Koumiss is rich in probiotics and many components that can alleviate the clinical symptoms of many diseases via the functional characteristics of koumiss and its regulation of intestinal flora. To investigate the antagonistic effect of koumiss on T. gondii infection, the model of acute and chronic T. gondii infection was established in this study. The survival rate, SHIRPA score, serum cytokine levels, brain cyst counts, β-amyloid deposition and intestinal flora changes were measured after koumiss feeding. The results showed that the clinical symptoms of mice were improved at 6 dpi and that the SHIRPA score decreased after koumiss feeding (P < 0.05). At the same time, the levels of IL-4, IFN-γ and TNF-α decreased (P < 0.001, P < 0.001, P < 0.01). There was no significant difference of survival rate between koumiss treatment and the other groups. Surprisingly, the results of chronic infection models showed that koumiss could significantly reduce the number of brain cysts in mice (P < 0.05), improve β-amyloid deposition in the hippocampus (P < 0.01) and decrease the levels of IFN-γ and TNF-α (P < 0.01, P < 0.05). Moreover, koumiss could influence the gut microbiota function in resisting T. gondii infection. In conclusion, koumiss had a significant effect on chronic T. gondii infection in mice and could improve the relevant indicators of acute T. gondii infection in mice. The research provides new evidence for the development of safe and effective anti-T. gondii methods, as well as a theoretical basis and data support for the use of probiotics against T. gondii infection and broadened thoughts for the development and utilization of koumiss.

612. GENDER DIFFERENCES IN JAPANESE PATIENTS WITH ESOPHAGEAL SQUAMOUS CELL CARCINOMA

Abstract Esophageal squamous cell carcinoma (ESCC) is the seventh most common cancer and the sixth leading cause of cancer-related death worldwide, and has a male preponderance. The gender-based differences in clinical presentation and risk factor exposure are not fully studied. The aim of this study was to compare males and females with this disease. We retrospectively investigated the clinical data of 503 consecutive patients with ESCC from April 2011 to December 2019. Data collected included patient demographics, clinical presentation, pathology and risk factor exposure. We analyzed the differences in clinical characteristics and risk factor exposure between males and females with ESCC. Four hundred and sixty-nine patients with ESCC were included. The male to female ratio was 4.6:1. There was no significant difference in clinical presentation and pathology. There were significant differences between risk factor exposure between the two genders: smoking and alcohol consumption was an association in more than 90% of males but in less than 50% of females. The analysis including all patients showed no significant difference in survival rates, but when limited to patients with stage 3 or higher disease, survival rates were significantly higher in females (Figure). Smoking and alcohol consumption, traditionally considered risk factors, are less associated with female patients with ESCC, and other environmental risk factors might be more involved in this gender.

Methylene Blue-Based Combination Therapy with Amodiaquine Prevents Severe Malaria in an Experimental Rodent Model.

Untreated malaria can progress rapidly to severe forms (<24 h). Moreover, resistance to antimalarial drugs is a threat to global efforts to protect people from malaria. Given this, it is clear that new chemotherapy must be developed. We contribute new data about using methylene blue (MB) to cure malaria and cerebral malaria in a combined therapy with common antimalarial drugs, including mefloquine (MQ) and amodiaquine (AQ). A C57BL6/J mouse model was used in an experimental cerebral malaria model. Mice were infected with Plasmodium berghei ANKA on Day 0 (D0) and the treatment started on D3 (nearly 1% parasitaemia) with AQ, MQ or MB alone or in combination with AQ or MQ. AQ, MQ and MB alone were unable to prevent cerebral malaria as part of a late chemotherapy. MB-based combination therapies were efficient even if treatment began at a late stage. We found a significant difference in survival rate (p < 0.0001) between MBAQ and the untreated group, but also with the AQ (p = 0.0024) and MB groups (p < 0.0001). All the infected mice treated with MB in combination with AQ were protected from cerebral malaria. Partial protection was demonstrated with MB associated with MQ. In this group, a significant difference was found between MBMQ and the untreated group (p < 0.0001), MQ (p = 0.0079) and MB (p = 0.0039). MB associated with AQ would be a good candidate for preventing cerebral malaria.

The short- and long-term survival of hyperthermic intraperitoneal chemotherapy (HIPEC) in the advanced gastric cancer with/without peritoneal carcinomatosis: a systematic review and meta-analysis of randomized controlled trials.

To evaluate the short- and long-term survival of hyperthermic intraperitoneal chemotherapy (HIPEC) in the patients with advanced gastric cancer (AGC) through randomized controlled trials (RCTs). We analyzed the endpoints of AGC patients including 1-, 2-, 3-, and 5-year overall survival (OS), intestinal anastomotic leakage, myelosuppression, nausea and vomiting from included studies. And we retrieved RCTs from medical literature databases. Risk ratios (RR) was used to calculated the endpoints. Totally, we retrieved 13 articles (14 trial comparisons) which contained 1091 patients. They were randomized to HIPEC group and control group. The results showed that there was no significant differences in survival rates between HIPEC group and control group at 1-, 2- and 3-year follow-up, while a statistical significant overall survival effect was found at the 5-year follow-up [RR: 1.20, 95% CI 1.01 to 1.43, I2 = 0.0%]. And there is no significant difference in the risk of intestinal anastomotic leakage, myelosuppression and nausea and vomiting. Compared with the control group, HIPEC could improve the long-term OS without increasing the risk of adverse effect in AGC patients with/without peritoneal carcinomatosis, but there was no benefit at short-term OS.

Effects of fecal bacteria on growth, digestive capacity, antioxidant capacity, intestinal health of large yellow croaker (Larimichthys crocea) larvae

Marine fish at early life is vulnerable to the threat of external pathogenic bacteria with unestablished intestinal flora. Early life intervention could contribute to healthy development of larvae gut tract. Consequently, the present study examined how large yellow croaker larvae (Larimichthys crocea) (mean body weight 3.64 ± 0.20 mg) responde to fecal microbiota came from 12-month-old large yellow croaker by oral administration. The intestinal contents of the donor were prepared into bacterial solution and sprayed on the Micro-diet. After 30 days feeding, growth performance, intestinal development, digestive ability and gut microbiota of larvae were detected. Results showed that there was no significant difference in survival rate. After fecal microbiota transplantation (FMT) treatment fish larvae had significantly higher final body weight, weight gain rate and specific growth rate. FMT treatment remarkably enhanced the activities of α-amylase and trypsin in intestinal segment and the activity of lipase and trypsin in pancreatic segment. Furthermore, FMT treatment significantly increased the activity of leucine-aminopeptidase on brush border membrane and the mRNA expression of pcna and occludin compared with the control group. Similarly, gut in larvae of FMT group had significantly higher villus height and muscular thickness. Moreover, FMT treatment significantly improved the activity of lysozyme and decreased malondialdehyde concentration in visceral mass. FMT treatment increased intestinal microbial diversities and altered intestinal microbiota profile of larvae. Spearman analysis revealed that FMT profoundly influenced the function of gut microbiota. Bacteria introduced by FMT may play a role in digestion and intestinal tract development. In conclusion, our results revealed that FMT treatment improved microbial diversity and modulated intestinal microecology of large yellow coraker larvae, which could significantly affect the digestive capacity, antioxidant capacity, intestinal development and intestinal health, thus improved its growth performance.

Association of intensive care unit or paediatric intensive care unit admissions with the method of transporting patients: a multicentre retrospective study

BackgroundReports regarding transportation methods of severely critical patients admitted to an intensive care unit (ICU) or paediatric ICU (PICU) are limited. In an attempt to address this research gap, this study aimed to test the hypothesis that prognosis is worse in patients transported by family members.MethodsThis multicentre study collected data from the Japanese Registry of Paediatric Acute Care database. Data concerning patients aged ≤16 years admitted to a participating hospital ICU or PICU and their transportation method to the hospital were extracted and divided into two groups: transported by family and transported by emergency medical services (EMS).ResultsOf the 2963 patients who met the criteria, 871 (29.4%) were transported by family and 2092 (70.6%) were transported by EMS. Significantly more patients with chronic conditions (551 patients, 63.3% vs. 845 patients, 40.4%; p < 0.01) or respiratory failure (414 patients, 47.5% vs. 455 patients, 21.7%; p < 0.01) were admitted to the ICU or PICU in the family transport group. There was no significant difference in survival rate between EMS and family transport group, matched by PIM2, chronic condition status and transport distance (OR:1.17, 95%CI:0.39–3.47, p = 0.78).ConclusionThe results of this study show that the transportation method does not affect the survival rate of paediatric patients. The proportion of patients with chronic conditions or those admitted because of respiratory failure was higher in the family transport group than in the EMS group. Therefore, as these patients are more likely to be admitted to the ICU or PICU, it is important to provide prompt respiratory care and medical interventions to achieve the best outcomes.

Screening of Differential Expression Autophagy Genes Related to the Prognosis of Diffuse Large B-Cell Lymphoma and Establishment of an Autophagy Model

To screen the differential expression of diffuse large B-cell lymphoma (DLBCL) autophagy-related gene (ARG), explore the mechanism of differential expression of autophagy gene (DEARG) in the occurrence and development of DLBCL and establish a prognostic model. Using the NCICCR database containing clinical information and gene expression profile data of 481 patients with DLBCL and the HADb database containing 232 ARGs, the differential expression of ARG in DLBCL was determined by R language, the relationship between ARG and the occurrence and development of DLBCL was analyzed by GO and KEGG, the polygene prognostic model was established by Cox regression algorithm, the survival curve was drawn by Kaplan-Meier method, and the reliability of the prognostic model was evaluated by ROC curve. A total of 122 DEARGs were extracted from lymph node samples of 481 patients with DLBCL and 5 normal lymph nodes, including 4 up-regulated genes and 118 down-regulated genes. GO enrichment mainly focused on ontological annotations such as mitochondrial autophagy, autophagy regulation, and cell response to external stimuli. KEGG enrichment was mainly concentrated in cell senescence, NOD-like receptor signal pathway, PI3K-Akt signal pathway, and PD-1/PD-L1 signal pathway. Survival analysis was performed on 230 samples with complete clinical information. Univariate Cox analysis showed that 20 ARGs were significantly correlated with overall survival of DLBCL patients. Nine prognostic ARGs (HIF1A, CAPN1, ITPR1, PRKCQ, TRAIL, HDAC1, TSC2, NRG3, and MAPK3) were screened by multivariate Cox regression to establish DLBCL ARG prognostic model. Kaplan-Meier survival curve analysis showed that there was significant difference in survival rate between high risk group and low risk group (P<0.001). Multivariate Cox regression analysis showed that international prognostic index and risk value were independent prognostic indicators of DLBCL patients (P<0.05), the area under ROC curve was 0.762 and 0.747, respectively. DLBCL ARG prognostic model can be used to predict the prognosis of patients, but it still needs to be confirmed by a large sample of clinical studies.

First feed matters: The first diet of larval fish programmes growth, survival, and metabolism of larval ballan wrasse (Labrus bergylta)

The use of cleaner fish, such as the ballan wrasse (Labrus bergylta), is important for combatting the sea lice problem in salmonid cage farming. Ballan wrasse is the only wrasse species that is cultivated, though only about 50% of the approx. 3 million ballan wrasse used in 2020 was produced by aquaculture. The major obstacle for large scale cultivation of ballan wrasse is the difficult first feeding phase and a lack of functional feeding protocols.Like most pelagic marine fish larvae, ballan wrasse do not accept inert diets as first feed, and feeding regimes based on rotifers and Artemia (brine shrimps) usually lead to mixed results and are far from being optimal nutrition for the larvae.In a 48-day start feeding experiment, we studied the feasibility of replacing rotifers by an Experimental cirriped diet or copepod nauplii (Acartia tonsa) and replacing Artemia by nauplii of the cirriped Semibalanus balanoides. Later, all treatments received the same formulated diets. We sampled larvae at each feed transition to analyze different response variables such as growth, morphometry, gene expression, lipidomics, histology, and microbiology.We found significant differences in survival rates and growth. Larvae fed copepods as the first diet had significantly higher survival rates than larvae start-fed on either rotifers or small experimental cirripeds, and this pattern was also reflected in early growth and bone development. Gut histology at the end of the experiment (48 days after hatching) showed a more developed intestinal tissue in the larval group fed copepods first and cirripeds as the second diet compared to the other larval groups.Gene expression at day 48 post hatch still revealed pronounced differences between the larval group first fed on rotifers and larvae from the other three feeding regimes which received natural, unenriched diets. Even weeks after receiving the same formulated diets, lipidomics analyses revealed that several lipid species correlated either negatively or positively with larval growth rates or mortality.Our results are a clear indication for nutritional programming, pointing towards the importance the first diet has for the further life of a fish.

Extranodal extension influences prognosis in pancreatic head cancer: A retrospective cohort study.

Extranodal extension (ENE) is an established prognostic factor in several gastrointestinal cancers. However, the prognostic impact remains unclear. Here, we investigated the prognostic implications of ENE in patients with surgically resected pancreatic cancer. We retrospectively reviewed 476 surgically resected pancreatic head cancer patients who consecutively underwent upfront pancreaticoduodenectomy for pathologically confirmed pancreatic ductal adenocarcinoma between January 2009 and December 2013. We compared the disease-free survival (DFS) rates of the patients according to ENE status. Among the 476 patients, patients with ENE had lower DFS rates than those without ENE (N0, 13 months; LN+/ENE-, 7months; LN+/ENE+, 6months; P < .001). In addition, even in the same N stage, patients with ENE had lower DFS rates than those without ENE (N0, 13 months; N1/ENE- 8months; N1/ENE+, 7months; N2/ENE-, 7months; N2/ENE+, 4months, P < .001). However, there was no significant difference in survival rates between patients in the N1/ENE+ group and those in the N2/ENE- group. Additionally, ENE was an independent prognostic factor for pancreatic cancer. Extranodal extension significantly predicted a poor prognosis among patients with pancreatic head cancer, especially those with nodal metastasis. Therefore, ENE should be considered a prognostic factor in future editions of the staging system.

Does the presence of physician-staffed emergency medical services improve the prognosis in out-of-hospital cardiac arrest? A propensity score matching analysis.

Substantial differences in survival after out-of-hospital cardiac arrest (OHCA) have been observed between countries. These might be attributed to the organization of emergency medical service (EMS) systems, including prehospital physician involvement. However, limited data exist on the physician's role in improving survival after OHCA. To compare prehospital and in-hospital outcomes of OHCA patients attended by physician-staffed EMS vs. paramedic-staffed EMS units. Among all patients enrolled in the regional, prospective registry of OHCA in southern Poland, we excluded those aged <18 years, with unwitnessed or EMS-witnessed cardiac arrest, without attempted cardiopulmonary resuscitation (CPR), attended by more than one EMS, or with traumatic cardiac arrest. The groups were matched 1:1 using propensity scores for baseline characteristic variables that might influence physician-staffed EMS dispatch. A total of 812 OHCA cases were included in the current analysis. Among them, 351 patients were attended by physician-staffed EMS. There were no differences in baseline characteristics in the propensity-score matched cohort consisting of 351 pairs. The return of spontaneous circulation (ROSC) was more often achieved in the physician-staffed EMS group (42.7% vs. 33.3%; P = 0.01). The prehospital survival rate was also higher in this group (34.1% vs. 19.2%; P <0.01). However, there were no significant differences in survival rate to discharge between cases treated by physician-staffed and paramedic-staffed EMS (9.7% vs. 7.0%; P = 0.22). OHCA patients attended by physician-staffed EMS were more likely to have ROSC and survive till hospital admission. However, better prehospital outcomes might not translate into improved in-hospital prognosis in these patients.

Genetic parameter estimation for resistance to red tide raphidophyte Chattonella antiqua in Japanese yellowtail Seriola quinqueradiata

Blooms of the harmful algal plankton Chattonella spp. cause severe economic damage to aquaculture. This study aimed to investigate the potential for selective breeding of resistance trait to Chattonella red tides in Japanese yellowtail Seriola quinqueradiata. Two experimental populations were prepared using different production procedures, one was produced by full factorial mating and common rearing tanks, and another was produced by pair mating and family separated rearing tanks. Fingerlings were exposed to mass cultured Chattonella antiqua and their mortality time was recorded as the phenotype of Chattonella resistance. There were significant differences in survival rate among full-sib families (range 0–50%). Heritability estimates of mortality time were moderate and similar in both populations as 0.329 ± 0.104 and 0.314 ± 0.118 respectively. The environmental variance caused by difference in rearing tanks was relatively small compared with the genetic variance, and had minimal influence on genetic parameter estimation. In contrast, body weight was highly influenced by the environmental variance of rearing tanks, thus heritability estimates varied between populations, 0.275 ± 0.102 and 0.153 ± 0.083 respectively. The genetic correlation between mortality time and body weight was not significant. This is the first report that reveals the contribution of a genetic difference to Chattonella resistance and indicates the potential for selective breeding of Chattonella resistance in Japanese yellowtail.

Application Effect of Robot-Assisted Laparoscopy in Hepatectomy for Colorectal Cancer Patients with Liver Metastases.

Objective Application effect of Leonardo's robot-assisted laparoscopy in hepatectomy for colorectal cancer patients with liver metastases. Methods A total of 122 patients with sCRLM treated in our hospital from May 2015 to June 2018 were selected and divided into observation group (n = 61) and control group (n = 61) according to random number table method. The observation group was treated with robot-assisted laparoscopic hepatectomy, while the control group was treated with conventional laparoscopic hepatectomy. The perioperative time, intraoperative blood transfusion, intraoperative blood loss, average intraoperative blood transfusion, and hepatic portal occlusion time of the two groups were observed. Serum cortisol (Cor), norepinephrine (NE), and glucose (Glu) levels were detected before and after surgery in the two groups. The oxygen consumption and carbon dioxide output of patients were measured 1 day before surgery and 1~3 days after surgery, and the resting energy expenditure (REE) value was calculated. The levels of CD3+, CD4+, and CD8+ were determined by flow cytometry. The incidence of complications was compared between the two groups. Patients were followed up for 3 years after discharge, and Kaplan-Meier method was used to analyze the survival of the two groups. Results The operation time, intraoperative blood transfusion, intraoperative blood loss, and average intraoperative blood transfusion in the observation group were all less than those in the control group, and the differences were statistically significant (P < 0.05). Three days after operation, the levels of serum Cor, NE, and Glu were increased in both groups, and the observation group was lower than the control group; the difference was statistically significant (P < 0.05). The REE level of observation group was lower than that of control group after 1 day, 2 days, and 3 days after surgery, and the difference was statistically significant (P < 0.05). Three days after operation, the levels of serum CD3+ and CD4+ were decreased in both groups, and the observation group was higher than the control group; the difference was statistically significant (P < 0.05). The incidence of complications in the observation group (3.28%) was lower than that in the control group (13.11%); the difference was statistically significant (P < 0.05).There was no significant difference in survival rate between the two groups after 1, 2, and 3 years of follow-up (P > 0.05). Conclusion The application of robot-assisted laparoscopy in patients with sCRLM can effectively improve the perioperative situation of patients, reduce stress, energy metabolism, and immune damage, and reduce the incidence of complications.

Induction Strategies in Lung Transplantation: Alemtuzumab vs. Basiliximab a Single-Center Experience.

BackgroundInduction therapy is used in about 80% of lung transplant centers and is increasing globally. Currently, there are no standards or guidelines for the use of induction therapy. At our institution, we have two induction strategies, basiliximab, and alemtuzumab. The goal of this manuscript is to share our experience and practice since this is an area of controversy.MethodsWe retrospectively reviewed 807 lung transplants performed at our institution between 2011 and 2020. Indications for the use of the basiliximab protocol were as follows: patients over the age of 70 years, history of cancer, hepatitis C virus or human immunodeficiency virus infection history, and cytomegalovirus or Epstein-Barr virus (donor positive/ recipient negative). In the absence of these clinical factors, the alemtuzumab protocol was used.Results453 patients underwent alemtuzumab induction and 354 patients underwent basiliximab. There were significant differences in delayed chest closure (24.7% alemtuzumab vs 31.4% basiliximab, p = 0.037), grade 3 primary graft dysfunction observed within 72 hours (19.9% alemtuzumab vs 29.9% basiliximab, p = 0.002), postoperative hepatic dysfunction (8.8% alemtuzumab vs 14.7% basiliximab, p = 0.009), acute cellular rejection in first year (39.1% alemtuzumab vs 53.4% basiliximab, p < 0.001). The overall survival rate of the patients with alemtuzumab induction was significantly higher than those of the patients with basiliximab induction (5 years survival rate: 64.1% alemtuzumab vs 52.3%, basiliximab, p < 0.001). Multivariate Cox regression analysis confirmed lower 5-year survival for basiliximab induction (HR = 1.41, p = 0.02), recipient cytomegalovirus positive (HR = 1.49, p = 0.01), postoperative hepatic dysfunction (HR = 2.20, p < 0.001), and acute kidney injury requiring renal replacement therapy (HR = 2.27, p < 0.001).ConclusionsIn this single center retrospective review, there was a significant difference in survival rates between induction strategies. This outcome may be attributable to differences in recipient characteristics between the groups. However, the Alemtuzumab group experienced less episodes of acute cellular rejection within the first year.

Impact of Surgery in De Novo Metastatic Breast Cancer after Systemic Disease Control

Background: Systemic chemotherapy is the standard of care for patients with metastatic breast cancer, with an undecided role in surgery. Limited data is available for the role of surgery on the overall survival of stage 4 breast cancer in regards to luminal subtypes of patients. This is a retrospective data analysis comparing overall survival benefit and disease-free survival in stage IV breast cancer after systemic treatment and systemic disease control concerning luminal classification in the last five years. Method: Patients who had surgery and no surgery after systemic treatment and disease control were compared for 5 years overall survival as the primary endpoint and disease-free survival as the secondary endpoint. The survival benefit was also compared in regards to tumor biology (ER/PR, HER2 status) Results: Data included 430 patients, 244 in surgery and 186 in no surgery group. At one year survival for surgery performed and not performed was not significant. Five-year overall survival for surgery performed and not performed was 84.4% and 74.6%. A statistically highly significant difference in survival rates was observed (p&lt;0.0001). The mortality rate was 15.6% in surgery performed and 25.4% in the no-surgery group which showed a significant difference among the two study groups (p=0.011). We found statistically significant differences in luminal B (p=0.01) and triple-negative breast cancer patients (p=0.001) for survival rates in surgery performed and not performed groups. Conclusion: Surgery has a positive impact on overall survival in Stage 4 patients with systemic disease control even in high-risk luminal B, Her 2 Positive, and triple-negative breast cancer patients. There was no significant difference observed in disease-free survival who were operated on or not. However, there was no local recurrence in the operated group. Keywords: Metastatic breast cancer: Neoadjuvant chemotherapy, local breast surgery, Luminal sub-types

Effects of the Clostridium butyricum on growth performance, antioxidant capacity, immunity and disease resistance of Litopenaeus Vannamei fed with cottonseed protein concentrate (CPC) replacement of fishmeal in diet

Clostridium butyricum (CB) is a gram-positive bacterium that secretes short-chain fatty acids such as butyric acid and so on. An 8-week feeding trial was conducted to investigate the effects of CB on the growth performance, antioxidant capacity, immunity and resistance to Vibrio parahaemolyticus in Litopenaeus Vannamei fed with cottonseed protein concentrate (CPC) replacement of fishmeal. Six iso-nitrogenous (40%) and iso-lipidic (6%) diets were formulated including a positive control group (PC, 25% fishmeal), a negative control group (NC, CPC replaced 30% of fishmeal protein), and 0.03% (C1, 3 × 108 CFU/kg), 0.12% (C2, 1.2 × 109 CFU/kg), 0.48% (C3, 4.8 × 109 CFU/kg) and 1.92% (C4, 1.92 × 1010 CFU/kg) CB were supplemented on the negative control group (NC). After the feeding trial, the remaining shrimp in each treatment group were subjected to a challenge experiment with Vibrio parahaemolyticus. The results indicated that weight gain rate (WGR), specific growth rate (SGR) in C4 group were significantly lower than those in PC and C2 groups (P < 0.05); the feed conversion ratio (FCR) was significantly higher than that of PC and C2 groups (P < 0.05). There was no significant difference in survival rate (SR) among all groups (P > 0.05). Compared to the PC and NC groups, the total superoxide capacity, superoxide dismutase and lysozyme were significantly higher in the C4 group (P < 0.05); the glutathione peroxidase, acid phosphatase and alkaline phosphatase were significantly higher in the C3 group (P < 0.05); and the malondialdehyde was significantly lower in the C4 group (P < 0.05). The relative mRNA expressions of Toll receptor (TLR), innate immune deficiency gene (IMD), penaiedin3a (Pen3) were significantly down-regulated in the NC group than those in the PC group (P < 0.05). In addition, the relative mRNA expressions of TLR, IMD and Pen3 were significantly up-regulated in all groups supplemented with CB than those in the NC group (P < 0.05). Moreover, the cumulative mortality rate in the NC group was not significantly different from the PC group (P > 0.05) and was significantly higher than those in the C3 and C4 groups (P < 0.05). In conclusion, the CB supplementation on the basis of CPC replacement of 30% fishmeal protein enhanced significantly the antioxidant capacity, immunity and disease resistance of shrimp and improved its growth performance. Therefore, considering the factors of the growth, immunity and disease resistance, the CB supplementation of 0.12%–0.48% (1.2 × 109 CFU/kg-4.8 × 109 CFU/kg) was recommended in the diet of L. vannamei based on the results of this experiment.

Molecular clustering based on gene set expression and its relationship with prognosis in patients with lung adenocarcinoma.

BackgroundLung adenocarcinoma (LUAD) is a subtype of lung cancer with high morbidity and mortality. While genotyping is an important determinant for the prognosis of LUAD patients, there is a paucity of studies on gene set-based expression (GSE) typing for LUAD. This current study used GSE methodology to perform gene typing of LUAD patients.MethodsClinical and genomic information of the LUAD patients were downloaded from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. Patients with LUAD were clustered into different molecular subtypes depending on the clinical and gene set expression characteristics. The survival rate and silhouette widths were compared between each molecular subtype. Differences in survival rate between gene sets were analyzed using Kaplan-Meier survival curves. Cox regression and Lasso regression were used to establish the prognostic gene set model based on the TCGA database, and the results were validated using the GEO dataset.ResultsA total of 10 hub genes were finally identified and clustered into 3 subtypes with a mean contour width of 0.96. There were significant differences in survival rates among the 3 subtypes (P<0.05). Gene Ontology (GO) analysis indicated that the related biological processes (BP) were mainly involved in regulation of cell cycle, mitotic cell cycle phase transition, and proteasome-mediated ubiquitin-dependent protein catabolic process. The cellular components (CC) were related to the spindle, chromosomal region, and midbody. Molecular function (MF) mainly focused on ubiquitin-like protein ligase binding, translation regulator activity, and oxidation activity. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the main pathways included the Epstein Barr virus infection pathway of neurogeneration, the p53 signaling pathway, and the proteome pathways. In addition, the protein-protein interaction network was analyzed using the STRING and Cytospace software, and the top 9 hub genes identified were KIF2C, DLGAP5, KIF20A, PSMC1, PSMD1, PSMB7, SNAI2, FGF13, and BMP2.ConclusionsPatients with LUAD can be clustered into three subtypes based on the expression of gene sets. These findings contribute to understanding the pathogenesis and molecular mechanisms in LUAD, and may lead to potential individualized pharmacogenetic therapy for patients with LUAD.