Significance In Dogs Research Articles

The diagnostic yield of preoperative screening for oral cancer in dogs over 15 years, part 2: distant screening.

Determine diagnostic yield of chest, abdomen, and 4-site screening to diagnose metastatic disease and secondary diseases of prognostic significance in dogs with oral cancer. Medical records from 381 dogs with histologically confirmed oral tumors that underwent preoperative screening were retrospectively reviewed. Thoracic metastasis was diagnosed in 4.9% (0.9% odontogenic, 6.5% nonodontogenic) of oral tumors. Oral malignant melanoma and multilobular osteochondrosarcoma were most at risk. Abdominal metastasis was diagnosed in 2% of oral tumors (0% odontogenic, 3.1% nonodontogenic) and cytologically confirmed in 2 cases (0.6% [2/295)] of all abdominal ultrasounds (AUS) 5.5% [2/36] of all AUS that had cytology). Both cases had oral malignant melanoma. Incidental disease was diagnosed in 53.1% and 81.3% of thoracic and abdominal screenings, respectively. Major findings were more common in AUS (7.8%) compared to thoracic screening (1.9%). The prevalence of incidental findings was similar for odontogenic and nonodontogenic tumors. Both metastasis and major findings were diagnosed more commonly with thoracic CT compared to radiographs. Metastasis or a major finding of prognostic significance was diagnosed in at least 1 test in 27.8% of patients that had head CT, lymph node cytology, thoracic screening, and AUS (n = 115). Major incidental findings were more commonly detected with AUS and were diagnosed in 1 in every 12 patients. However, metastatic disease was most commonly detected with thoracic screening. When all 4 screening tests are performed, there is an approximately 1 in 4 chance of diagnosing metastasis or major significant disease regardless of tumor type.

Gene expression of Nectin-4 and its clinical significance in dogs with primary lung adenocarcinoma.

BackgroundCanine primary lung adenocarcinoma (CPLA) is suspected by radiography or computed tomography; however, since there are no tumour markers, early diagnosis is difficult, and the prognosis is poor due to increased tumour volume. Nectin‐4 has been reported to be expressed in human lung, ovarian, and pancreatic cancers and promotes tumour growth. It has been reported to be a tumour marker and prognostic factor, and oncolytic virotherapy is being investigated using nectin‐4 as a therapeutic target.ObjectivesThe purpose of this study was to investigate the expression of Nectin‐4 in CPLA and its clinical significance in dogs with pulmonary adenocarcinomas.MethodsThe relationships between Nectin‐4 expression and signalling, tumour volume, tumour weight, and prognosis were analyzed in 34 CPLA patients.ResultsThe expression of canine Nectin‐4 (high Nectin‐4) was found in 25 of 34 cases (73%), and Nectin‐4 expression levels did not show any significant associations with gender, body weight, and tumour stage. However, there was a significant positive correlation between Nectin‐4 expression and tumour volume (r = 0.623, p < 0.05) and tumour weight (r = 0.735, p < 0.05). Regarding prognosis, the median survival time was 427 days in high Nectin‐4 cases and 420 days in cases with no Nectin‐4 expression.ConclusionOur study demonstrated that Nectin‐4 is highly expressed in CPLA. In addition, nectin‐4 might be a tumour growth factor in CPLA and thus is a promising biomarker for CPLA. Further investigations on nectin‐4 in CPLA are warranted for its diagnosis and novel targets for oncolytic virotherapy.

Comparative analysis of the molecular subtype landscape in canine and human mammary gland tumors

Breast cancers in humans belong to one of several intrinsic molecular subtypes each with different tumor biology and different clinical impact. Mammary gland tumors in dogs are proposed as a relevant comparative model for human breast cancer; however, it is still unclear whether the intrinsic molecular subtypes have the same significance in dogs and humans. Using publicly available data, we analyzed gene expression and whole-exome sequencing data from 158 canine mammary gland tumors. We performed molecular subtyping using the PAM50 method followed by subtype-specific comparisons of gene expression characteristics, mutation patterns and copy number profiles between canine tumors and human breast tumors from The Cancer Genome Atlas (TCGA) breast cancer cohort (n = 1097). We found that luminal A canine tumors greatly resemble luminal A human tumors both in gene expression characteristics, mutations and copy number profiles. Also, the basal-like canine and human tumors were relatively similar, with low expression of luminal epithelial markers and high expression of genes involved in cell proliferation. There were, however, distinct differences in immune-related gene expression patterns in basal-like tumors between the two species. Characteristic HER2-enriched and luminal B subtypes were not present in the canine cohort, and we found no tumors with high-level ERBB2 amplifications. Benign and malignant canine tumors displayed similar PAM50 subtype characteristics. Our findings indicate that deeper understanding of the different molecular subtypes in canine mammary gland tumors will further improve the value of canines as comparative models for human breast cancer.

A wolf in sheep’s clothing: The development of livestock guarding dogs in the Adriatic region of Croatia

Since their domestication, dogs have adapted to a diverse portfolio of roles within human societies, and changes in dog size, shape, and behavior are often key indicators of these changes. Among pastoral and agropastoral societies dogs are almost ubiquitous as livestock guardians and herding aids. Archaeological data demonstrate that incoming Neolithic farmers brought with them their own morphologically distinct dogs when they spread into Europe, and that these dogs became larger in the Bronze and Iron Ages. Using archaeological data from the eastern Adriatic region we suggest that changes in the morphology and treatment of dog remains by these societies reflect, in part, the significance of dogs in livestock management including guarding herds kept at distances from villages. Bronze and Iron Age increases in body size, in particular, may track the increasing importance of seasonal transhumance.

The prognostic value of admission point-of-care testing and modified Glasgow Coma Scale score in dogs and cats with traumatic brain injuries (2007-2010): 212 cases.

To investigate the association between admission point-of-care parameters and survival to discharge in dogs and cats with traumatic brain injury (TBI). Retrospective study 2007-2010. A rural university teaching hospital and an urban private practice teaching hospital. One hundred thirty-one dogs and 81 cats admitted to the emergency service with evidence of head injury based on history from the owner or physical exam. In dogs, nonsurvivors had significantly higher glucose concentrations at admission than survivors (median=8.49 mmol/L [153mg/dl] vs 6.83mmol/L [123mg/dl], p=0.039). In cats, there was no significant difference in admission glucose between survivors and nonsurvivors (median=10.21mmol/L [184mg/dl] vs 10.93mmol/L [197mg/dl], p=0.17). Modified Glasgow Coma Scale (MGCS) score was available for 105 of the 131 dogs (80%) and was significantly higher in survivors than in nonsurvivors (median=16 vs 11, p<0.0001). MGCS was available for 45 of the 81 cats (56%) and was significantly higher in survivors than in nonsurvivors (median=17vs 14, p=0.0005). The relative prognostic value of the admission point-of-care testing parameters and MGCS were assessed using a stepwise linear regression model, which included admission glucose, pH, base excess, sodium, and MGCS. In dogs, only admission glucose was an independent predictor of survival (odds ratio=1.027, 95% confidence interval, 1.0042-1.05, p=0.019). These results suggest that, as in people with TBI, increased blood glucose concentrations may have prognostic significance in dogs with TBI but not in cats. In addition, MGCS score may be predictive of survival in both dogs and cats with TBI.

Dog and human from Raven's perspective: An interpretation of Raven myths of Alaskan Athabascans

Raven appears as an important character in many Alaskan Athabascan myths. He is depicted as a powerful shaman who transforms the world through magic. In this paper, I analyze an often-neglected motif of Alaskan Athabascan Raven stories: Raven as Dog-eaters. I use mythological texts and ethnographic data collected in prior studies and from my fieldwork in the community of Nikolai, Alaska. In some Alaskan Athabascan myths, Raven has a strong appetite for dog meat and assists his neighbors who slaughter their dogs for him. This motif seems to describe a sacrificial practice whereby Raven, as a transformer, receives gifts of food in exchange for providing spiritual services and protection. However, in real life, Northern Athabascans do not practice typical animal sacrifice involving domestic animals. In addition, they do not regard dogs as being suitable for human consumption. This raises several questions. What is the significance of dogs in Alaskan Athabascan societies? Why is Raven thought to be fond of dog meat in traditional Alaskan Athabascan stories? How can we explain this motif of “Dog as Raven's Delicacy” through an ethnography of human–dog–raven relations in Alaskan Athabascan societies? This paper addresses these questions from the perspectives of comparative mythology, ethology and the storytelling strategy.

Effect of peri-operative crystalloid fluid rate on circulating hyaluronan in healthy dogs: A pilot study

Hypervolemia can damage the endothelial glycocalyx, a key regulator of vascular permeability, coagulation and inflammation. A starting peri-operative crystalloid fluid rate of 5mL/kg/h is recommended for healthy dogs undergoing elective procedures but higher rates continue to be commonly used. This study aimed to determine if a higher starting perioperative fluid rate was associated with a greater increase in plasma concentrations of hyaluronan, a marker correlated with glycocalyx damage, in systemically healthy dogs undergoing elective surgical procedures.Based on a sample size calculation, 38 dogs undergoing ovariohysterectomy or castration were randomly assigned to receive lactated Ringer's at a starting perioperative fluid rate of 10mL/kg/h (n=19) or 5mL/kg/h (n=19). Plasma hyaluronan concentrations were measured by ELISA in pre- and post-fluid therapy samples. There were no significant differences between groups in hyaluronan values before (baseline, P=0.52) or after perioperative fluid administration (P=0.62). Compared to respective baseline values, hyaluronan values significantly increased following 5 and 10ml/kg/h fluid administration (P=0.02 for both comparisons). This preliminary study identified an increase in hyaluronan over the course of fluid therapy with both the low and high fluid rate. One possible explanation is that both fluid rates contribute to glycocalyx disruption, but it should be emphasized that hyaluronan is not specific to the glycocalyx. Further studies are needed to determine the origin of the increased circulating hyaluronan and its clinical significance in dogs undergoing elective surgical procedures.

Prognostic significance of midline shift of the olfactory or frontal lobes of the brain in canine nasal carcinomas treated by palliative radiotherapy: a pilot study

Canine nasal carcinomas are often treated with radiotherapy. Presence of lysis of the cribriform plate by tumor invasion (stage 4 by modified Adams’s staging system) is a well-known prognostic factor. In this study, dogs with stage 4 disease were divided into two subgroups based on the presence or absence of midline shift of the olfactory or frontal lobes of the brain (Stage 4a: without presence of midline shift. Stage 4b: with midline shift). The median survival time of dogs with midline shift was significantly shorter than that of dogs without midline shift (64 vs. 208 days). Our results indicate that the finding of a midline shift might have a prognostic significance in dogs with nasal carcinoma treated with radiotherapy.

Evaluation of the delta neutrophil index from an automated blood cell analyser in septic dogs

Immature granulocytes (IG) are a marker of severe inflammatory states in human beings and animals, and have been linked to a diagnosis of sepsis and poor prognosis. The delta neutrophil index (DNI), automatically calculated by a haematological analyser, provides an estimate of circulating IG. In particular, an increased DNI value has been associated with the severity of sepsis, and mortality, in critically ill human beings. The aims of this study were to determine the DNI reference interval (RI) in healthy dogs, and to evaluate its diagnostic and prognostic significance in dogs with sepsis. A total of 118 dogs with sepsis undergoing a complete blood cell count (CBC) at the time of hospital admission were included retrospectively. Dogs with sepsis were compared to 20 dogs with primary immune-mediated haemolytic anaemia (IMHA) and 99 healthy controls. The DNI RI was set from 0 to 9.2%. The DNI was significantly higher in dogs with sepsis compared to dogs with IMHA and healthy dogs (P<0.001), and significantly higher in dogs with septic shock compared to septic dogs without circulatory failure (P<0.03). No differences were detected between survivors (78/118) and non-survivors (40/118). Septic dogs with a DNI above the RI had significantly higher frequencies of IG and toxic neutrophil changes on manual blood smear evaluation (P=0.03 and P<0.001, respectively). The DNI had a fair performance in identifying dogs with sepsis in this population and predicted septic shock. Larger prospective studies are needed to validate DNI measurement in dogs and to test its clinical utility.

Retrospective evaluation of circulating thyroid hormones in critically ill dogs with systemic inflammatory response syndrome

Critical illness can be associated with transient alterations in circulating thyroid hormone concentrations, indicating the presence of non-thyroidal illness (NTI). NTI is well described in humans, but there are few reports on its occurrence and prognostic significance in dogs. This retrospective study assessed the occurrence of NTI in a population of dogs with systemic inflammatory response syndrome (SIRS) and investigated its association with disease severity (APPLEfast scores). A total of 41 SIRS dogs were included and were divided by SIRS origin (non-septic SIRS, n = 10; septic SIRS, n = 41) and final outcome (survivors, n = 37; non-survivors, n = 4). Healthy, age-matched dogs (n = 15) were included as controls. Serum thyroid hormone levels including total T3, free T3, total T4, and reverse T3 were measured upon admission. Compared to controls, there were significant changes in serum thyroid hormone concentrations in SIRS dogs, suggesting the presence of NTI. Septic SIRS dogs had higher APPLEfast scores and lower serum thyroid hormones concentrations than those in non-septic SIRS and control dogs. In conclusion, NTI was frequent in dogs with SIRS and may be associated with the presence of sepsis or high illness severity.

Prevalence of gastrointestinal helminth parasites of zoonotic significance in dogs and cats in lower Northern Thailand.

Gastrointestinal zoonotic helminths of dogs and cats have a public health concern worldwide. We investigated the prevalence of gastrointestinal helminths of zoonotic significance in dogs and cats in lower Northern Thailand and utilized molecular tools for species identification of hookworms and Opisthorchis viverrini. Fecal samples of 197 dogs and 180 cats were collected. Overall prevalence of infection using microscopy was 40.1% in dogs and 33.9% in cats. Helminth infection found in both dogs and cats included hookworms, Spirometra spp., Taenia spp., Toxocara spp., O. viverrini, Strongyloides spp. and Trichuris spp. Hookworms were the most common helminth in dogs, while Spirometra spp. were the most prevalent in cats. Among hookworm infection in dogs and cats, Ancylostoma ceylanicum was the most prevalent hookworm, being 82.1% in hookworm infected dogs and 95.8% in hookworm infected cats. Mixed-infection due to hookworms and Spirometra spp. was the most dominant in both dogs and cats. Our finding showed that zoonotic helminth infection is highly prevalent in dogs and cats in the lower Northern area of Thailand.

Causes of anaemia other than acute blood loss and their clinical significance in dogs

To identify the causes of anaemia, other than acute blood loss, in dogs and to determine whether severity of anaemia provides clues to the diagnosis. The veterinary medical database of the Veterinary Campus Hospital, Lyon was searched. Dogs with anaemia (packed cell volume <37%) were included and assigned to different disease groups. Dogs with acute blood loss were excluded. The case records were examined for weakness at presentation, the severity and regeneration of anaemia and the final diagnosis including tumour type if applicable. The case records of 456 dogs with low packed cell volume were included. Cancer-related anaemia and anaemia of inflammatory disease accounted for 33·1 and 28·5% of cases, respectively. Most dogs with cancer-related anaemia had solid tumours (73%). The prevalence of immune-mediated anaemia increased with severity of anaemia (5·3, 15·5, 41·2 and 56·2% for mild, moderate, severe and very severe anaemia, respectively), whereas the prevalence of anaemia of inflammatory disease decreased (36·7, 22·5, 2·9 and 0% for mild, moderate, severe and very severe anaemia, respectively). Anaemia of inflammatory disease and cancer-related anaemia were the most frequently identified causes of anaemia in dogs. The percentage of dogs with immune-mediated anaemia increased with anaemia severity, whereas the percentage of dogs with anaemia of inflammatory disease decreased with anaemia severity. Thus, severity of anaemia may provide clues to the diagnosis.

Development of multiple pigmented viral plaques and squamous cell carcinomas in a dog infected by a novel papillomavirus

Canine viral plaques are uncommon skin lesions that are induced by papillomaviruses (PVs). Plaques are usually of little clinical significance in dogs, although they have been reported rarely to progress to squamous cell carcinoma (SCC). Here is described a 7-year-old mixed-breed dog that developed numerous darkly pigmented plaques up to 8 cm in diameter. Multiple ulcerated nodular masses were visible within plaques on the ventrum and axilla. The dog showed no clinical evidence of immunodeficiency and appeared otherwise healthy. Over the next 2 years, five surgeries were performed to remove 23 ulcerated masses that ranged in size from 2 to 5 cm in diameter. Five masses were submitted for histology, and all were SCCs. Each was surrounded by epidermis that contained histological features consistent with those described in canine plaques. Suggestive of a PV aetiology, massive numbers of large keratohyaline granules were present throughout the thickened epidermis. Additionally, koilocytes were focally present, and one sample contained a band of keratinocytes within the superficial epidermis that contained pale cytoplasm and marginated chromatin. From two samples, DNA sequences from a previously unreported PV were amplified, and immunohistochemistry confirmed the presence of PV antigen in both. The PV DNA sequences were most similar to those of canine PVs previously associated with plaque formation. The plaques observed in this case were unusual owing to their rapid growth, large size and frequent malignant transformation. It is unknown whether this unusual behaviour was due to the specific PV detected in this case or to host factors within the dog.

Pearsonema (syn Capillaria) plica associated cystitis in a Fennoscandian arctic fox (Vulpes lagopus: a case report

The bladderworm Pearsonema (syn Capillaria) plica affects domestic dogs and wild carnivores worldwide. A high prevalence in red foxes (Vulpes vulpes) has been reported in many European countries. P. plica inhabits the lower urinary tract and is considered to be of low pathogenic significance in dogs mostly causing asymptomatic infections. However, a higher level of pathogenicity has been reported in foxes. A severe cystitis associated with numerous bladderworms was found in a captive arctic fox (Vulpes lagopus) originating from the endangered Fennoscandian arctic fox population. To our knowledge this is the first description of P. plica infection in an arctic fox.

Neorickettsia helminthoeca and salmon poisoning disease: A review

Neorickettsia helminthoeca is an obligate intra-cytoplasmic bacterium that causes salmon poisoning disease (SPD), an acute, febrile, fatal disease of dogs. The complex life-cycle of this pathogen involves stages in an intestinal fluke (Nanophyetus salmincola), a river snail (Oxytrema silicula), in fish, and in fish-eating mammals. This complexity has created confusion with respect to the various bacterial and parasitic infections associated with the disease and its significance in dogs in specific geographical locations has likely to have previously been under-estimated. This paper addresses the history, taxonomy, microbiology of N. helminthoeca and summarises the pathogenesis, clinical signs and pathological features associated with infection. Furthermore, the biological cycles, treatment, control, and both public and veterinary health impacts associated with this pathogen and the intestinal fluke N. salmincola are discussed.

Changing Functions of the Canine Image in Venetian Religious Paintings of the Sixteenth Century

This paper demonstrates how Venetian Renaissance artists perpetuated the symbolic contexts of animal symbolism, which they disguised under the veil of genre and narrative in religious art. Venetian painters of the Cinquecento shared an unprecedented penchant for depicting animals in general, and dogs in particular, in their secular and religious iconography. A dramatic pattern of change is demonstrated in their approach to canine iconography, Titian consistently adopted the negative moralistic connotations of the canine image, which had been transmitted to the Renaissance by medieval literature and art. It is demonstrated, by contrast, that the significance of dogs in themes of sacred art, as depicted by Tintoretto, are related to classical sources illustrating positive conceptions of the canine character and faculties. In literature of the sixteenth century the dog was increasingly cast in an authoritative role, attesting to miraculous and supernatural events by virtue of its superior perception or exemplifying the ideal of rationality. Classical sources also provided prototypes for canine epitaphs and laudatory poems in sixteenth century writings. While writers of the period praised canine cleverness, rationality, alertness, loyalty, vigilance, memory, prudence, and vision, Venetian artists were depicting the virtuous canine in allegorical contexts. By the late 1540s and 1550s, when Tintoretto began introducing canine images into his religious paintings, many of the relevant sources on animals had become available through the Venetian printing press.

Pattern of Coombs’ test reactivity has diagnostic significance in dogs with immune‐mediated haemolytic anaemia

To investigate the clinical significance of the pattern of Coombs' test reactivity in dogs with immune-mediated haemolytic anaemia. Sixty-five anaemic dogs with a positive Coombs' test were included. Coombs' testing was performed at 4 and 37 degrees C with polyvalent canine Coombs' reagent and antisera specific for each of canine immunoglobulin G, immunoglobulin M and complement factor C3. The impact of performing testing with only polyvalent antiserum at 37 degrees C was assessed. Chi-squared tests were used to compare Coombs' test reactivity in dogs with primary immune-mediated haemolytic anaemia (group A) and in dogs with concurrent/underlying disease (group B). Following Bonferroni correction, significance was set at P < or = 0.003. Eleven dogs would have been regarded as Coombs' negative had they been tested with polyvalent antiserum at 37 degrees C alone. Group A dogs were significantly more likely to be positive with polyvalent antiserum and/or anti-dog immunoglobulin G at 4 and/or 37 degrees C (P < or = 0.001) and tended to be less likely to be positive with anti-dog immunoglobulin M at 4 degrees C (P=0.040). Testing of anaemic dogs with polyvalent Coombs' reagent at 37 degrees C was less sensitive than testing with monovalent reagents at 4 and 37 degrees C. The pattern of Coombs' test reactivity differed significantly between dogs with primary immune-mediated haemolytic anaemia and those with concurrent/underlying disease.

Characterization of pericardial ET-1 and it's functional significance in dogs

Characterization of pericardial ET-1 and it's functional significance in dogs

Aboriginal dogmatics: canines in theory, myth and dogma

Canines in Australian Aboriginal society have turned out to be a con siderable puzzle to anthropology. The role and significance of dogs, and traditionally dingoes, in this society had previously been so taken for granted as to be beyond reasonable doubt. In recent years, however, a lively, though sometimes dogged, debate has developed that demon strates otherwise. The debate is fired by more or less radical departures from the economic-utilitarian perspective that attributes to canines a decisive usefulness in man's food quest. Indeed this is usually seen as the primary reason for the animal's domestication. The traditional anthropological opinion holding that dingoes must have been economically useful to Aborigines as hunting aids, hinges on the assumption that man always and everywhere has been interested in the dog's domestication because he realised the animal's economic potential. Domestication, so it appears, is the outcome, as it were, of a rational' decision made in relation to the natural environment and