Eyes Of Shrimp Research Articles

Bound States in the Continuum Help Shrimp Eyes to Catch More Light

Optical bound states in the continuum (BICs) have recently attracted a great deal of attention as an efficient way to localize and manipulate light at nanoscale. Traditionally, generation of BICs has relied on using artificial structures where suppression of radiative losses leads to very high Q factors. Here, we show that BICs may play an important biological role by boosting light–matter interactions in a biogenic nanostructure: tapetum reflector of a shrimp eye. Enveloping photosensitive units of the retina (rhabdoms), this system contains quasi-periodic arrays of spherical core–shell nanoparticles which include concentric lamellae of single-crystal isoxanthopterin nanoplates arranged around a hollow core. The radial alignment of the plates gives rise to the spherical anisotropy of the nanoparticles which provides access to quasi-BIC modes in a full visible domain. Thus, a tapetum reflector hosting BICs maximizes light interactions with rhabdoms, enhancing the eye’s sensitivity. Our findings suggest that BICs, previously associated with man-made structures only, can be generated in biogenic structures, performing crucial optical functionalities in living organisms.

Characterization and Comparison of Eye Development and Phototransduction Genes in Deep- and Shallow-Water Shrimp Alvinocaris longirostris and Palaemon carinicauda

The investigations of the molecular components of eye development and phototransduction in deep-sea species are important to elucidate the mechanism of their adaptation to dim light. In this study, eye transcriptomes of the shrimp Alvinocaris longirostris from the deep-sea chemosynthetic ecosystem and the shallow-water shrimp Palaemon carinicauda were compared. Two Pax6 homologs with low expression levels were identified in both species, which are essential transcription factors in eye development. This finding implies that the development of the two shrimp eyes at early embryo–larvae stages might be similar. The multiple components of the phototransduction pathway were identified in both species. However, the number of phototransduction components was significantly reduced in A. longirostris, as well as expression level. Particularly, short-wavelength/UV-sensitive (SWS/UVS) opsins were absent in A. longirostris and only one putative middle-wavelength-sensitive (MWS) opsin was identified in this species. The conserved sites and structures of the putative LWS opsins were found between deep-sea and shallow-water decapods, indicating that the opsins in deep-sea crustaceans may also conserve their spectral absorption and signal transduction function. Phylogenetic analyses supported the monophyly of LWS opsins and SWS/UVS opsins in arthropods, while the MWS clade fell outside of the main arthropod LWS clade. The results are expected to provide baseline for study of visual adaptation in deep-sea shrimps.

仿生虾蛄眼偏振特性结构研究 (简讯)

仿生虾蛄眼偏振特性结构研究 (简讯)

A bio-inspired model for bidirectional polarisation detection

This study investigated a novel polarisation detection model based on the microstructure of rhabdom in mantis shrimp eyes, in which a single unit can detect two directions of orthogonal polarisation. The bionic model incorporated multi-layered orthogonal Si wire grids, and the finite-difference time-domain method was used to simulate light absorption. A single-layer Si wire grid was simulated to study the effects of thickness and duty cycle on extinction ratios. A multi-layer orthogonal wire grid was simulated to study the effects of distance between adjacent layers. The simulations revealed that the bionic model can achieve orthogonal polarisation detection. Additionally, for 600 coupled layers, the extinction ratios in both directions were greater than 60, and light absorption in the absorptive directions exceeded 96%.

Insights into the morphology of symbiotic shrimp eyes (Crustacea, Decapoda, Palaemonidae); the effects of habitat demands.

Morphometric differences in the optical morphology of symbiotic palaemonid shrimps can be observed among species symbiotic with different host organisms. Discriminant functional analysis revealed three distinct groups within the species examined. Of these, bivalve symbionts appear to have an eye design that is solely unique to this host-symbiont grouping, a design that spans across multiple genera of phylogenetically unrelated animals. Although some taxonomic effects may be evident, this does not explain the difference and similarities in eye morphology that are seen within these shrimps. Therefore evolutionary pressures from their host environments are having an impact on the optical morphology of their eyes however, as indicated by host-hopping events there ecological adaptations occur post host invasion.

Effect of green and clear water and lipid source on survival, growth and biochemical composition of Pacific white shrimp Litopenaeus vannamei

Despite the shrimp ability to obtain additional nutrients from food organisms endogenously produced within the green water system has been suggested as one of the causes for the better performance of Pacific white shrimp reared in green water in comparison with clear water, the nutritional components responsible for these effects have yet to be determined. The present study aims to understand the importance of natural food organisms in zero-water exchange systems as source of essential fatty acids for the Pacific white shrimp Litopenaeus vannamei. Five treatments were tested: two conducted in mesocosms systems with shrimp-fed diets containing either fish oil (FO) or olive oil, and another three conducted in clear water with shrimp-fed diets containing either olive oil, a docosahexaenoic acid (DHA)-rich oil or an arachidonic acid (ARA)-rich oil. The presence of higher levels of fatty acids 16:1n-7, 17:1, 20:4n-6, 20:3n-3 and 22:5n-6, characteristic of floc lipids, in shrimp reared in mesocosms denoted their assimilation from the floc. Substitution of FO by olive oil in diets for shrimp reared in mesocosms did not affect growth or survival. Survival and growth of shrimp reared in mesocosms was better than those reared in clear water and fed an olive oil diet, whereas DHA or ARA enrichment of non-fish oil (NFO) diet improved survival of shrimp reared in clear water. Higher survival rate, triglyceride and DHA content in whole body and eyes of shrimp fed a DHA-rich diet suggests that under these conditions, in clear water, it is necessary to include at least 4.8 g kg )1 DHA in diet dry weight. ARA enrichment seemed to negatively affect growth. The nutritional contribution of the floc to shrimp in mesocosm culture reduces or eliminates the need for a dietary source of FO and illustrates the importance of DHA and ARA to enhance shrimp survival in clear water conditions.

Effects of prenyl pyrophosphates on the binding of PKCgamma with RACK1.

Receptors for activated C kinase (RACKs) are a group of PKC binding proteins that have been shown to mediate isoform-selective functions of PKC and to be crucial in the translocation and subsequent functioning of the PKC isoenzymes on activation. RACK1 cDNA from the shrimp Penaeus japonicus was isolated by homology cloning. The hepatopancreas cDNA from this shrimp was found to encode a 318-residue polypeptide whose predicted amino acid sequence shared 91% homology with human G(beta2)-like proteins. Expression of the cDNA of shrimp RACK1 in vitro yielded a 45-kDa polypeptide with positive reactivity toward the monoclonal antibodies against RACK1 of mammals. The shrimp RACK1 was biotinylated and used to compare the effects of geranylgeranyl pyrophosphate and farnesyl pyrophosphate on its binding with PKCgamma in anti-biotin-IgG precipitates. PKCgammas were isolated from shrimp eyes and mouse brains. Both enzyme preparations were able to inhibit taxol-induced tubulin polymerization. Interestingly, when either geranylgeranyl pyrophosphate or farnesyl pyrophosphate was reduced to the submicrogram level, the recruitment activity of RACK1 with purified PKCgamma was found to increase dramatically. The activation is especially significant for RACK1 and PKCgamma from different species. The observation implies that the deprivation of prenyl pyrophosphate might function as a signal for RACK1 to switch the binding from the conventional isoenzymes of PKC (cPKC) to the novel isoenzymes of PKC (nPKC). A hydrophobic binding pocket for geranylgeranyl pyrophosphate in RACK1 is further revealed via prenylation with protein geranylgeranyl transferase I of shrimp P. japonicus.