Short Report Research Articles

METTL3 alters capping enzyme expression and its activity on ribosomal proteins.

The 5' cap, catalyzed by RNA guanylyltransferase and 5'-phosphatase (RNGTT), is a vital mRNA modification for the functionality of mRNAs. mRNA capping occurs in the nucleus for the maturation of the functional mRNA and in the cytoplasm for fine-tuning gene expression. Given the fundamental importance of RNGTT in mRNA maturation and expression there is a need to further investigate the regulation of RNGTT. N6-methyladenosine (m6A) is one of the most abundant RNA modifications involved in the regulation of protein translation, mRNA stability, splicing, and export. We sought to investigate whether m6A could regulate the expression and activity of RNGTT. In this short report, we demonstrated that the 3'UTR of RNGTT mRNA is methylated with m6a by the m6A writer methyltransferase 3 (METTL3). Knockdown of METTL3 resulted in reduced protein expression of RNGTT. Sequencing of capped mRNAs identified an underrepresentation of ribosomal protein mRNA overlapping with 5' terminal oligopyrimidine (TOP) mRNAs, and genes are dysregulated when cytoplasmic capping is inhibited. Pathway analysis identified disruptions in the mTOR and p70S6K pathways. A reduction in RPS6 mRNA capping, protein expression, and phosphorylation was detected with METTL3 knockdown.

When clotting falters: vitamin K deficiency in short gut syndrome case report

When clotting falters: vitamin K deficiency in short gut syndrome case report

A Short Report on a Single-center Survey of Barium Acute Appendicitis.

A Short Report on a Single-center Survey of Barium Acute Appendicitis.

Poor face recognition predicts social anxiety in autism: A short report.

Research has shown that some autistic people have severe difficulties in recognising other people's faces. However, little is understood about how these difficulties impact the daily life and the mental well-being of autistic people. In this study, we asked 60 autistic adults with varying degrees of face recognition ability to complete two tests of face recognition, a questionnaire about social anxiety and a bespoke survey which asked participants about their experiences of face recognition and social interaction. We found that participants who had poor face recognition reported experiencing higher levels of social anxiety compared to those with average or better face recognition skills. More than half felt that their face recognition difficulties affected their social interactions, and over a third believed it hindered their ability to make friends. These findings suggest that face recognition difficulties may contribute to social anxiety among autistic individuals.

A short report of a Scottish audit of disability and child protection medical examinations

AbstractA Scottish national child protection audit has shown that practitioners can have differing understanding of the term ‘disability’ and are variable in their own recording of disabilities. It was apparent from the audit that disability was more likely to be recorded for older children than those under five. The audit found that an abuse allegation is less likely to be substantiated for disabled children by a Child Protection Medical Examination. For disabled children in our sample, there was a ‘substantiation of abuse rate’ of 33%, which was less than the 56% rate for non‐disabled children. The differences in rates encourage us to consider what is an appropriate response when disabled children may have experienced harm and abuse. The audit highlights the vulnerability of disabled children to abuse and indicates the need to equip practitioners with the skills, competencies and confidence to engage with and support this vulnerable group.

Health literacy in pregnant women facing prenatal screening: a short report from “SaperePer” project

Abstract Advancements in health promotion and prenatal medicine have made noninvasive prenatal testing (NIPT) a common practice during pregnancy, but understanding prenatal health information becomes crucial not only for the well-being of the mother but also for that of the unborn child. Analyze health-related information during pregnancy is fundamental, and this work fits perfectly with this topic, reporting preliminary results from the pilot study “SaperePer”. A health-related questionnaire was administered to 358 women in the first trimester of pregnancy, aiming to study different outcomes: 1.level of knowledge of NIPT; 2.number of screening tests undergone; 3.willingness to undergo a diagnostic test with a high outcome risk of screening test; 4.willingness to end the pregnancy following a diagnosis of pathology. The questionnaire was divided into 3 sections: personal characteristics and lifestyle habits, prenatal health behavior scale (PHBS), and knowledge of NIPT. Preliminary results showed that the level of knowledge of NIPT was higher in older, atheist, working, wealthy highly educated, and preliminary informed women, as well as the higher number of screening tests undergone and the propensity to undergo an invasive diagnostic test after a high-risk screening result (KS and MW tests p < 0.05). On the contrary, women with lower education, faithful and, even more, not preliminary and appropriately informed during prenatal counseling showed lower knowledge of NIPT, lower number of tests undergone, and meager chances to undergo an invasive diagnostic test or to end the pregnancy after a positive diagnosis of pathology (KS and MW tests p < 0.05). In conclusion, this study evaluated health-related information during the first but crucial stages of pregnancy, opening a new understanding of how health literacy and correct prenatal counseling could increase awareness and health promotion in pregnant women, particularly in those women with lower cultural tools. Key messages • Health literacy and correct prenatal counseling could increase awareness and health promotion in pregnant women. • Understanding prenatal health information becomes crucial both for maternal and prenatal well-being.

Loss of Surfactant Protein A Alters Perinatal Lung Morphology and Susceptibility to Hyperoxia-Induced Bronchopulmonary Dysplasia

Bronchopulmonary dysplasia (BPD) is a condition of poor alveolar formation that causes chronic breathing impairment in infants born prematurely. Preterm lungs lack surfactant and are vulnerable to oxidative injuries driving the development of BPD. Our recent studies reported that surfactant protein A (SP-A) genetic variants influence susceptibility to neonatal lung disease. SP-A modulates activation of alveolar macrophages and parturition onset in late gestation. We asked whether a lack of SP-A alters alveolarization in a mouse model of hyperoxia-induced BPD. SP-A-deficient and control newborn mice were exposed to either clinically relevant 60% O2 hyperoxia or normoxia for 5–7 days. Alveolar formation was then assessed by mean linear intercept (MLI) and radial alveolar count (RAC) measurements in lung tissue sections. We report that the combination of SP-A deficiency and hyperoxia reduces alveolar growth compared to WT mice. The morphometric analysis of normoxic SP-A-deficient lungs showed lower RAC compared to controls, indicating reduced alveolar number. In the presence of hyperoxia, MLI was higher in SP-A-deficient lungs compared to controls. Differences were statistically significant for female pups. Spatial proteomic profiling of lung tissue sections showed that hyperoxia caused a 4-fold increase in the DNA damage marker γH2Ax in macrophages of SP-A-deficient lungs compared to normoxia. Our short report suggests an important role for SP-A in perinatal lung development and the protection of lung macrophages from oxidant injury. These studies warrant future investigation to discern the temporal interaction of SP-A, gender, oxidant injury, and lung macrophages in perinatal alveolar formation and development of BPD.

Investigation of Inhibitory Effect of Sheep Lymph Nodes and Antibiotics on Cutibacterium Acnes, Staphylococcus Aureus, and Staphylococcus Epidermidis in the Laboratory Condition: A Short Report

Investigation of Inhibitory Effect of Sheep Lymph Nodes and Antibiotics on Cutibacterium Acnes, Staphylococcus Aureus, and Staphylococcus Epidermidis in the Laboratory Condition: A Short Report

Total Artificial Heart Implantation as a Bridge to Transplantation in Slovakia.

Although left ventricular assist device implantation represents the majority of durable mechanical circulatory support implants for patients with advanced heart failure, as many as 20 to 30% will subsequently have right heart failure requiring extended inotropic support or short-term mechanical circulatory support, and the total artificial heart is an established tool in the bridge to transplant armamentarium. The aim of this short report is to present our center's experience with the use of SynCardia total artificial heart. Between November 2017 and April 2021, 10 SynCardia total artificial heart devices were implanted. Of the 10 patients who underwent total artificial heart implantation, 6 (60%) were successfully bridged to transplant with a median time of 6.5 (interquartile range [IQR] 6-8) months, and 4 patients died on device support during the index hospitalization. The 30-day, 1-year, and 3-year survival rates after heart transplantation were the same at 66.7% (4/6). Despite the uncertain future of total artificial hearts, it remains a viable option for patients who require biventricular bridge to transplant or for a select subset of patients with advance heart failure who may not otherwise survive.

Comparative Analysis of Reddit Posts and ChatGPT-Generated Texts’ Linguistic Features: A Short Report on Artificial Intelligence’s Imitative Capabilities

In recent years, the unprecedented explosion of artificial intelligence (AI), particularly generative AI, has dramatically and drastically altered many human fields, posing queries about how generative AI can imitate human language. Given the newness of generative AI as a controversial phenomenon, there is an urgency to closely examine how its linguistic outputs could mimic human language produced in natural contexts. Hence, in this short report, we discuss the observed similarities and differences in the linguistic features of the subreddit r/Marriage spouse appreciatory posts and ChatGPT-4 outputs. These results were the offshoot of our genre analysis on these two linguistic data sets. Our analysis revealed that ChatGPT-4 generated texts contain impeccable grammar, while the Reddit appreciatory posts have grammatical discrepancies, such as errors in subject-verb agreement, improper punctuation marks, and erroneous capitalization; ChatGPT-4 generated texts have more complex syntactical structure; Reddit dataset utilized more internet jargon, slang, and profanities and seems to be unpredictable and arbitrary in terms of textual length; and ChatGPT-4 outputs appear to overuse emojis while underuse emoticons and tend to use these digital linguistic elements without regard to their proper contexts. In light of these results, we claim that AI-generated texts, although they can mimic human language, this is on a mere surface level, and a closer inspection could uncover distinct variations. We recommend that future studies use more comprehensive and different datasets and continuously employ comparative and contrastive linguistic analysis to further investigate AI’s imitative capabilities.

Short report: Prison-reported rates of autism in female prisons in England

Short report: Prison-reported rates of autism in female prisons in England

229 Evaluation of the Global Matrix of Para Report Cards on Physical Activity of Children and Adolescents with Disabilities

Abstract Purpose Physical activity provides a wide range of health benefits for children and adolescents with disabilities (CAWD). However, despite CAWD being at higher risk of physical inactivity, there is a lack of surveillance systems that capture physical activity data of CAWD. To address this gap, the Global Matrix of Para Report Cards on physical activity of CAWD was set up with 14 participating countries/jurisdictions (Ng et al., 2023) based on the methodology of the Active Healthy Kids Global Alliance’s Global Matrix 4.0 (Aubert et al., 2023). This study aims to evaluate the process, outcome, and impact of the Global Matrix of Para Report Cards. Methods The evaluation was informed by the Global Matrix 3.0 evaluation process (Aubert et al., 2020). Process, outcome, and impact indicators were informed by online surveys, reports/publications, and website analytics of the special issue published in Adapted Physical Activity Quarterly. Results Two online surveys have been completed by 64-71% of targeted respondents. Moderate-to-high satisfaction rates were reported for the development of and participation in the Global Matrix initiative and open-ended comments reported issues (e.g., data availability, inconsistency in definitions) and positive feedback (e.g., networking/collaboration, visibility). The participating Report Card teams assigned and contributed a total of 139 physical activity grades—overall physical activity, organised sport, active play, active transport, physical fitness, sedentary behaviour, family and peers, schools, community and environment, government—to the Global Matrix of which 45% were incomplete. Twelve Report Card teams published a short Para Report Card article for their country. As of February 2024, >47,000 views have been recorded for the special issue comprising the Global Matrix and country Para Report Card articles. Conclusions This evaluation highlights the need for more discussion on the definitions of disability and benchmarks of physical activity indicators to improve the grading process and more support in accessing and synthesising data related to CAWD. These are important steps to support inclusion and representation of CAWD in (inter)national surveillance of physical activity and health.

Self-poisoning with paracetamol in England: short report of characteristics of individuals and their overdoses according to source of tablets.

Self-poisoning with paracetamol is the most frequently used overdose method in the UK. Psychosocial assessments were conducted by mental health clinicians with 127 consecutive individuals who presented with pure paracetamol overdoses to a large general hospital over 8 months, including asking about the source of the tablets and scoring the patients' acts on the Beck Suicide Intent scale (BSI). Patients were predominantly female (86%) and young (79% aged 12-24 years). Most had used paracetamol which was available in the home (77%). Those who purchased paracetamol for the act took double the number of tablets compared with those who used paracetamol available in the home (37 v. 18), had higher suicidal intent (mean BSI: 11 v. 7) and more often required treatment with N-acetyl cysteine (71% v. 43%). These results highlight the need for safer home storage of paracetamol and consideration of reducing pack size limits on paracetamol that can be purchased.

In-Vivo MRI in Rodents: A Protocol for Optimal Animal Positioning.

Magnetic resonance imaging (MRI) is a potentially powerful novel peripheral nerve diagnosis technique. To determine its validity, in-vivo preclinical studies are necessary. However, when using a rodent model, positioning rats and achieving high-resolution images can be challenging. We present a short report that outlines an optimal protocol for positioning rats for in-vivo MRI acquisition. Female Sprague-Dawley rats with sciatic nerve injury were induced into anesthesia using 4% isoflurane in oxygen and maintained at 1.5%. Rats were placed into a plexiglass cradle in a right lateral recumbent position, and a surface coil was placed over the left leg. Respiration rate and body temperature were monitored throughout the scan. Our protocol was successful as rats were able to undergo MRI scanning safely and efficiently. There were no adverse reactions, and clear images of the left sciatic nerve were obtained. Animal positioning took 30 minutes, and 5 different acquisitions were obtained in 2 hours. The total time from anesthesia induction to recovery was under 3 hours. Given the increasing interest in MRI diagnostic techniques, we hope this report aids other researchers studying peripheral nerve injury imaging in rat models.

Interprofessional heart failure education for self-care knowledge and medication adherence in cardiac rehabilitation patients: A short report

Heart failure (HF), a costly and often deadly syndrome, is associated with hindered cardiac functioning. Suboptimal HF disease knowledge and related poor self-care practices result in worsening heart failure, increased rehospitalizations, decreased quality of life, and potential death. Provision of interprofessional education is essential to encourage disease understanding and reliable adoption of appropriate self-care behaviors. This short report describes the timely implementation of an evidence-based practice educational project (EBP) designed to augment heart failure knowledge and vital self-care management strategies, among phase II cardiac rehabilitation (CR) patients. Individual sessions led by interprofessionals included nursing, advanced nursing, nutrition, information technology, and exercise physiology clinicians. Post-intervention results suggest collaborative interprofessional patient education effectively enhanced knowledge (19.1 % increase) about overall heart failure and specifically improved medication adherence rates (5.4 % increase). Efficient and effective interprofessional self-care-focused education should become routine practice in phase II CR patients to address complex care challenges, decrease costs and improve overall outcomes.

Pilot study to assess the early cardiac safety of carbon ion radiotherapy for intra- and para-cardiac tumours.

Modern photon radiotherapy effectively spares cardiac structures more than previous volumetric approaches. Still, it is related to non-negligible cardiac toxicity due to the low-dose bath of surrounding normal tissues. However, the dosimetric advantages of particle radiotherapy make it apromising treatment for para- and intra-cardiac tumours. In the current short report, we evaluate the cardiac safety profile of carbon ion radiotherapy (CIRT) for radioresistant intra- and para-cardiac malignancies in areal-world setting. We retrospectively analysed serum biomarkers (TnI, CRP and NT-proBNP), echocardiographic, and both 12-lead and 24-hour Holter electrocardiogram (ECG) data of consecutive patients with radioresistant intra- and para-cardiac tumours irradiated with CIRT between June 2019 and September 2022. In the CIRT planning optimization process, to minimize the delivered doses, we contoured and gave ahigh priority to the cardiac substructures. Weekly re-evaluative 4D computed tomography scans were carried out throughout the treatment. Atotal of 16patients with intra- and para-cardiac localizations of radioresistant tumours were treated up to atotal dose of 70.4 Gy relative biological effectiveness (RBE) and amean heart dose of 2.41 Gy(RBE). We did not record any significant variation of the analysed serum biomarkers after CIRT nor significant changes of echocardiographic features, biventricular strain, or 12-lead and 24-hour Holter ECG parameters during 6months of follow-up. Our pilot study suggests that carbon ion radiotherapy is apromising radiation technique capable of sparing off-target side effects at the cardiac level. Alarger cohort, long-term follow-up and further prospective studies are needed to confirm these findings.

Emerging Patterns of Foot and Ankle Injuries in Pickleball Players: A Short Report.

Pickleball's surging popularity has driven an increase in injuries presenting to medical providers. This study seeks to describe the epidemiology of pickleball foot and ankle injuries including patient demographics, diagnoses, and mechanism of injury. A retrospective review from our institutional database identified patients treated in the foot and ankle clinic whose medical records included the search terms "pickleball" and "pickle ball." Only injuries sustained while playing pickleball were included. Patient demographics, diagnosis, mechanism of injury, and treatment were collected. Injury incidence and descriptive analyses were calculated. A total of 198 patients with pickleball foot and ankle injuries were identified. The incidence of injuries increased 6.5-fold from 2019 to 2023. The mean age of patients was 58.3 years (SD = 12.2). Most patients were male (58.6%) and reported a traumatic injury (77.8%). The most common diagnosis was Achilles tendon rupture (39.4%). The most common mechanisms of injury were running or lunging forward (30.9%), planting the foot (16.5%), and inverting the foot and ankle (15.5%). Most injuries were treated nonoperatively (71.2%); however, 62.8% of Achilles tendon ruptures were treated surgically. The incidence of pickleball foot and ankle injuries increased dramatically from 2015 to 2023. Injuries occurred more frequently in older, male patients, with Achilles tendon rupture being the most common diagnosis.

Cleaning time and motion: an observational study on the time required to clean shared medical equipment in hospitals effectively

Despite the important role that cleaning plays in reducing the risk of healthcare-associated infections, no research has been undertaken to quantify the time required for effective cleaning and disinfection of different pieces of shared medical equipment commonly used in hospitals. This short report presents the results from a study that aimed to quantify the time required to clean common pieces of shared medical equipment effectively. An observational time and motion study was conducted in a nursing simulation laboratory to determine the time required for effective cleaning and disinfection of 12 pieces of shared medical equipment commonly used in hospital. After training, the participants cleaned and disinfected equipment, with the time taken to clean recorded. Cleaning was deemed to be effective if ≥80% of ultraviolet fluorescent dots were removed during the cleaning process. The time to clean equipment effectively ranged from 50s [blood glucose testing kit; 95% confidence interval (CI) 0:40-1:00 (min:s)] to 3min 53s [medication trolley; 95% CI 3:36-4:11 (min:s)]. The intravenous stand was cleaned most effectively, with 100% of dots removed (N= 100 dots). In contrast, the bladder scanner was the most difficult to clean, with 12 attempts required to meet the 80% threshold for effective cleaning. This study will inform staffing and training requirements to plan the cleaning and disinfection of shared medical equipment effectively. The findings can also be used for business cases, and in future cost-effectiveness evaluations of cleaning interventions that include shared medical equipment.

Assessing the Veterinary Health Care Needs of Knoxville’s Latino Community Through a Community-Based Participatory Approach: A Short Report

The primary objective of the study was to better understand the interests, needs, and perceived barriers to veterinary care of Knoxville’s Latino community. Investigators adapted a national survey designed to understand access to veterinary care for distribution to the Latino population through a community-based participatory approach with an established community center. Seventy-six percent of survey respondents (n = 46) reported that they consider the pet as part of the family. Forty-eight percent said their pets were not spayed or neutered (s/n), and of these, 39% said s/n was cost-prohibitive, whereas 22% did not know where to receive services. Twenty-six percent identified an inability to obtain treatment for a sick pet, primarily due to a lack of access to affordable care. Participants expressed interest in vaccines and parasite prevention (64%), training and behavior (36%), and information on veterinary and pet care resources (29%). The survey tool (provided in English and Spanish) and community engagement approach can assist animal welfare organizations in identifying and addressing community veterinary needs and barriers to care.

Association of the Achondroplasia Foramen Magnum Score and intraoperative neuromonitoring

IntroductionForamen magnum stenosis in achondroplasia carries a risk of sudden death. A proportion of these patients benefit from foramen magnum decompression (FMD). The Achondroplasia Foramen Magnum Score (AFMS) was developed...