Short Processing Time Research Articles

Monomer extraction from polymers using supercritical CO2

Polymerization reactions often leave unreacted monomers in the polymer. These unreacted monomers can emit vapors at ambient temperatures that cause unpleasant odors, pose both environmental and safety risks, and negatively impacts polymer properties. Therefore, a purification step is necessary after polymerization to remove these residual monomers. High temperature devolatilization is the currently applied technique of removing these monomers, however this treatment can also negatively impact the properties of the polymer. In this study the possibility of using supercritical CO2 (scCO2) as an extraction medium of these residual monomers, which are considered as Volatile Organic Compounds (VOCs), has been investigated experimentally. Polymer samples of either PS or PMMA with 4–7 wt% of residual monomers were extracted continuously with scCO2 at temperatures ranging from 50 to 90 °C and pressures of 100–400 bar. The measured extraction efficiencies, based on 1H NMR analysis, were 99+% and was reached between 4 and 16 h depending on the experimental conditions. In general, higher temperatures and higher pressures lead to enhanced extraction rates and thus higher efficiencies at shorter process times. The experimental results were successfully modelled with an empirical extraction simulation model as originally proposed by Sovová.

Clearing the plate: a strategic approach to mitigate well-to-well contamination in large-scale microbiome studies.

Understanding dynamic microbial communities typically requires large-scale studies. However, handling large numbers of samples introduces many challenges, with cross-contamination being a major issue. It not only complicates analysis but also leads to sample loss and increased costs and restricts diverse study designs. The prevalent use of 96-well plates for nucleic acid and metabolite extractions exacerbates this problem due to their wells having little separation and being connected by a single plate seal. To address this, we propose a new strategy using barcoded Matrix Tubes, showing a significant reduction in cross-contamination compared to conventional plate-based approaches. Additionally, this method facilitates the extraction of both nucleic acids and metabolites from a single tubed sample, eliminating the need to collect separate aliquots for each extraction. This innovation improves large-scale study design by shortening processing times, simplifying analysis, facilitating metadata curation, and producing more reliable results.

Rapid Microwave Annealing for Improved Crystallinity and Morphology of Perovskite Materials

Perovskite solar cells are gaining significant attention for their remarkable power conversion efficiency, cost‐effective processing, and material abundance. This study investigates the impact of rapid microwave annealing on the crystallinity and morphology of MAPbI3 films on FTO glass substrates. Multifaceted characterization techniques, including field emission scanning electron microscopy (FESEM), X‐ray diffraction (XRD), UV–Vis spectroscopy, photothermal deflection spectroscopy (PDS), and steady‐state photoluminescence (PL) measurements are used to compare microwave‐annealed samples with traditional hotplate‐annealed samples. Microwave annealing yields significantly larger crystals in shorter processing times, suggesting enhanced crystallinity, as evidenced by SEM analysis and XRD data. UV‐Vis and PDS measurements indicate improved optical properties and reduced sub‐bandgap states, while PL results suggest diminished nonradiative recombination in microwave‐annealed samples. However, a partial film detachment has been observed at higher microwave powers, a phenomenon explained by COMSOL simulations. These findings demonstrate rapid microwave annealing as an energy‐efficient and cost‐effective alternative while highlighting the need for further optimization to address film degradation issues, which remain a significant challenge. This research supports the potential for scalable, high‐quality perovskite material production, facilitating large‐scale production and commercialization of next‐generation solar cells.

Alternative drying method for chontaduro pulp (Bactris gasipaes) by pulsed microwaves: Physicochemical analysis and kinetic model

Chontaduro (Bactris gasipaes), is a species of palm common in Central and South America. This perishable fruit is classified as a drupe. The objective of this research was to evaluate pulsed-microwave drying effect on physicochemical properties and kinetic models of two chontaduro varieties (yellow and red). The results showed that fresh yellow and red chontaduros contained 55.01 % and 64.6 % of moisture, and 6.85 % and 7.03 % of protein, respectively. Both pulsed-microwave and convective drying (control) successfully produced dehydrated materials with moisture contents below 10 % and water activities around 0.2, indicating stable products. The pulsed-microwave drying required significantly less time to reduce the water content in the fruits than convective process. In drying kinetics, all mathematical models were suitable to describe the experimental data, with the Page model showing the highest accuracy according to R2 and lowest RSEM and χ2 values. pH, acidity, fat, fiber, and ash content did not show changes after drying. However, thermally sensitive compounds, as protein and β-carotenes, were affected. Samples after pulsed-microwave drying presented 3.63 % and 5.19 % of protein for yellow and red samples, respectively. β-carotenes were reduced until to 55.51 % by pulsed-microwave and 56.96 % for convective drying. Pulsed-microwave drying is an alternative method to obtain dried products with appropriate physicochemical properties, where the heat mechanism does not adversely affect food characteristics during a short processing time. The non-traditional drying methods are a viable alternative to obtain new dried products from conventional or non-conventional sources with good physical, chemical and nutritional properties, as an increasingly demanding market.

Design of a bifunctional prepolymer for simultaneously facile preparation and highly efficient electrodialysis of anion exchange membranes

A facile fabrication process for separation membranes is crucial to decrease the production cost, while the photo-polymerization approach can be conducted at ambient conditions with short processing time and low energy consumption. Herein, this work designs a bifunctional prepolymer for simultaneously facile preparation and highly efficient electrodialysis of poly (2,6-dimethyl-1,4-phenylene oxide) (PPO) anion exchange membranes. PPO is selected as the main skeleton of prepolymer because of the excellent mechanical and thermal stability. The dimethylaminoethyl methacrylate (DMAEMA) is grafted with brominated PPO by quaternization, where it contains cationic groups, i.e. quaternary ammonium, and photopolymerized groups, i.e. methacrylate C = C. This resulting prepolymer can be fast polymerized under UV irradiation via C = C from DMAEMA. This structure is synergistically regulated by the amounts of crosslinker DMAEMA and modifier N-bromosuccinimide. The optimized membrane exhibits an excellent ion exchange capacity of 2.19 mmol g−1, a water uptake of 38.17 %, and a tensile strength of 4.8 MPa. Meanwhile, its electrolyzer cell performance achieves a density of 580 mA cm−2 at an adjusted voltage of 2.1 V, and the desalination rate of seawater during electrodialysis reaches 98.34 %. This work provides a universal and facile fabrication route for high-performance AEMs.

Visa policy and international student migration: evidence from Canada

Abstract This article examines how visa policy affects international student migration. Using administrative data on community colleges in Canada, we evaluate a reform that introduced a new visa stream—the Student Partners Program (SPP)—with shorter processing times and higher approval rates for student visa applicants able to demonstrate that they have the financial resources and language skills to succeed academically. Using a triple difference estimator, we find that SPP increased student migration from treated countries by 34% relative to what would have occurred without the reform, and that this effect materialized only among students from origin countries suffering from low approval rates. Moreover, we show that higher enrollment was driven in roughly equal proportions by an increase in visa approval rates and the volume of visa applications, suggesting that the policy helped reduce statistical discrimination and increased the attractiveness of treated institutions. We also leverage the SPP reform to investigate potential crowding-out effects. While inflows of international students did not reduce the number of domestic students enrolled at Canadian community colleges, the reform helped foreign enrollment outpace domestic enrollment by crowding-in international students from countries that were not eligible to SPP.

A novel route to produce metal or ceramic parts in space: local debinding and sintering of powdered filaments

AbstractIn-space manufacturing of polymer feedstocks has already been shown using the widely investigated filament extrusion additive manufacturing (AM) technology. Yet, polymers are only a small piece of the puzzle, and there is a growing demand to locally source metal and ceramic parts. In this manuscript, we propose a cost-effective method for in-orbit manufacturing of metal and ceramic multi-material components using highly packed powdered filaments, which need to be shaped, debinded, and sintered in sequential steps. Traditional debinding and sintering of material extrusion (MEX) AM parts are known to be time-consuming and require complex post-processing, often involving toxic debinding agents. To overcome this, a low-intensity infrared diode laser and an induction heater are coupled to a hybrid MEX system to allow full processing in situ, within the same volume. The results show that the main binder matrix can be removed across the 3D volume of the part via laser ablation of the polymeric mass, even for multi-material metal–ceramic composites. The sintered geometries further densify efficiently within the bulk due to the high-energy concentration of the induction sintering treatment, providing short processing times. Debinding and sintering locally, in the same machine, offer a simple and effective way to produce space hardware in situ, avoiding the use of consumables or part transportation to bulky equipment.

Progress in essential oil extraction and their applications in food packaging and preservation

AbstractEssential oils (EOs) are secondary metabolites synthesised by many aromatic plants, which have good antimicrobial and antioxidant activities making them popular functional agents for food preservation and packaging applications. In recent years, novel extraction techniques, such as super critical fluid extraction, pulsed electric field assisted extraction and ultrasound assisted extraction have been explored as alternatives to the conventional solvent‐based extraction methods. This review mainly discusses on recent progresses in sustainable extraction of essential oil (EO) from aromatic plants and food processing wastes and their functional properties, and applications in food preservation have also been summarised. Sustainable extraction techniques yield better‐quality EO due to low temperature and short time process compared to the conventional extraction. Despite efficacy of EOs as good active agents, their low water solubility, volatile nature, strong flavour, and susceptibility to food processing parameters are major challenges against their applications in food. These challenges can be mitigated by advanced techniques such as encapsulation and nanoemulsification of EO, which provide dual functions of enhanced applicability and improved functional properties. Incorporation of EO in the biopolymer‐based films and coatings can open avenues for sustainable food packaging and preservation.

Rapid identification, pathotyping and quantification of infectious bursal disease virus by high-resolution melting curve quantitative reverse transcription PCR analysis: An innovative technology well-suited for real-time large-scale epidemiological surveillance

With the virus continuing to evolve, very virulent IBDV (vvIBDV) and novel variant IBDV (nvIBDV) have become the predominant epidemic strains in China, exacerbated by the widespread use of attenuated vaccine strains (attIBDV), making a complex infection situation of IBDV in the field. Therefore, developing a rapid and accurate high-resolution melting curve quantitative reverse transcription PCR (HRM-qRT-PCR) for the identification and pathotyping of IBDV is crucial for clinical monitoring and disease control. Extensive data analysis and genome-screening of the three dominant IBDV pathotypes identified a specific region (nucleotides 2450–2603 in segment A) with distinct GC content as the detection target. Experimental testing of HRM-qRT-PCR revealed distinct melting curves and high sensitivity, with the detection limits of 61.2 copies/μL, 61.1 copies/μL and 67.5 copies/μL for vvIBDV, nvIBDV and attIBDV, respectively. The method exhibited excellent specificity, with no inter-genotypes cross-reactivity among the three pathotypes and no reactivity to other common avian pathogens. Applied to samples with double and triple co-infections of different IBDV pathotypes, the method displayed specific melting peaks corresponding to the viruses present in the samples, with an accuracy rate of 100 %. This method precisely identifies and differentiates all the single or co-infected samples, generating distinct peaks corresponding to the Tm values of each virus pathotype in traditional melting curve plots. Furthermore, the method overcomes the limitations of traditional pathotyping methods, requiring only one reaction to achieve rapid viral pathotyping and facilitating quantitative analysis of viruses within the samples. This study introduces an innovative HRM-qRT-PCR method, offering new technology to rapid and accurate identification, pathotyping and quantification of vvIBDV, nvIBDV, and attIBDV. With strong discriminatory power, user-friendliness and a short processing time, this method is highly attractive for the rapid IBDV pathotyping in real-time large-scale epidemiological surveillance during outbreaks.

Multi-physics coupling numerical simulation of variable porosity in reactive melt infiltration process

Abstract The hypersonic craft necessitates significant advancements in thermal shielding to safeguard against the extreme aero-thermal environments encountered during flight at hypersonic speeds in near space or upon reentry from space. Carbon fiber-reinforced ceramic matrix composites (FRCMCs) have attracted increasingly interesting attention as advanced structural materials for application in thermal protection. The reactive melt infiltration (RMI) process is preferred for the preparation of FRCMCs because of its low cost and short processing time. However, the RMI process includes the complex interplay of melt flow, reaction dynamics, and thermal fields, with existing models failing to accurately predict these processes due to their uncoupled nature and neglect of pore structure changes. This study introduces a coupled model that integrates mass transfer flow, chemical reaction, and heat transfer, incorporating variable pore structures to more accurately evaluate the RMI process. This approach allows for a comprehensive assessment of internal pressure, temperature, and porosity changes during silicon infiltration into porous media, marking an improvement over the previous model. The model has been validated for use in predicting the exotherm of RMI processes. In conclusion, this coupled model presents a promising avenue for enhancing the fabrication of FRCMCs, either considering the melt infiltration from multiple locations in the RMI process or adjusting the pore structure at the infiltration location to larger pores.

Securing the Internet of Things with Ascon-Sign

With a Cryptographically-Relevant Quantum Computer (CRQC) estimated to be viable within the next 15 years, the development of post-quantum security is imperative. Previously secure networks may soon fall victim to these CRQCs as they will likely attack the weakest link in a network. In modern networks, these weak-links are often present in the form of Internet of Things (IoT) devices, as the resource constrains imposed by these wireless nodes leads to lowered security. We offer the first Ascon-Sign implementation for resource constrained FPGAs, which allows a wireless sensor network to verify nodes. Our design runs twice as fast as similarly-area constrained devices, and shows a 33% reduction in power per operation. We demonstrate the capability of our design by integrating it with a wireless sensor network for weather detecting. We also propose an amendment to the Ascon-Sign specification that allows for shortened processing time and lower memory requirements.

Effect of pilot-scale high-temperature short-time processing on the retention of key micronutrients in a fortified almond-based beverage: implications for fortification of plant-based milk alternatives.

The effect of thermal processing treatments on key micronutrients in fortified almond-based beverages has not been well characterized. An almond-based beverage was produced in a pilot plant, fortified with vitamin A palmitate, vitamin D2, riboflavin (vitamin B2), calcium carbonate, and zinc gluconate, and was processed using various high-temperature short-time (HTST) pasteurization treatments. Naturally present micronutrients in the base ingredients included several B vitamins (vitamin B1 [thiamin], total vitamin B3 [sum of nicotinamide and nicotinic acid], and total vitamin B6 [sum of pyridoxal, pyridoxamine, and pyridoxine]) and minerals (magnesium, phosphorus, and potassium). The prepared almond-based beverage was homogenized and thermally processed using HTST pasteurization with a temperature range from ~94 to 116°C for a constant time of 30 s. The samples were analyzed for vitamin A palmitate, vitamin D2, target B vitamins (thiamin, riboflavin, total vitamin B3, and total vitamin B6), and minerals (magnesium, phosphorus, potassium, calcium, and zinc). The results showed that amounts of vitamin A, vitamin D2, riboflavin, and total vitamin B6 did not significantly (p > 0.05) change after the HTST treatments, whereas thiamin significantly (p < 0.05) decreased by 17.9% after HTST treatment at 116°C. Interestingly, total vitamin B3 content significantly (p < 0.05) increased by 35.2% after HTST treatment at 116°C. There was no effect of processing on the minerals that were monitored. The results from this study indicate that the majority of key micronutrients assessed in this study are stable during HTST processing of an almond-based beverage and that fortification of plant-based milk alternatives may be a viable process to enhance the micronutrient content consumers receive from these products.

Cell-free expression and biochemical characterization of polysaccharide-synthesizing glycosyltransferases

Polysaccharides like cellulose and hyaluronan are synthesized by membrane-bound family-2 glycosyltransferases (GTs) that play critical structural, metabolic, or functional roles in cells. Though GT-2 family has the maximum number of deposited gene sequences, the biochemistry is poorly understood due to enzyme production challenges. Here, we developed a cell-free expression (CFE) protocol to produce two GT-2 family representative cellulose synthase (PttCesA8 from Populus tremula x tremuloides) and hyaluronan synthase (SeHAS from Streptococcus equisimilis). The CFE products were obtained as reconstituted proteoliposomes directly at high yields and short processing times compared to other approaches. Enzyme expression was confirmed using SDS-PAGE and immunoblotting, while integration of GTs into lipid layers was observed using cryogenic electron microscopy. Both GTs were catalytically active with Michalis-Menten kinetic constants, Km for PttCesA8, was 295.8 µM, and SeHAS was 321.51 µM (toward UDP N-Acetyl Glucosamine) and 207.88 µM (toward UDP Glucuronic Acid), respectively, and with UDP inhibiting both GTs. Mutation of conserved residues in SeHAS also confirmed the importance of lysine-139, glutamine-248, and threonine-283 residues in hyaluronan biosynthesis. In summary, CFE methods enable expression of polysaccharide-synthesizing enzymes as proteoliposomes at high yields with relative ease for rapid biochemical and structural characterization studies.

Safe and Reliable Movement of Fast LiDAR-based Self-driving Vehicle

Classification of objects is an important technique for autonomous ground vehicles to identify a surrounding environment and execute safe path planning. In this paper, a method based on horizontal segmentation is proposed to detect cone-shaped objects in vehicle’s vicinity using a LiDAR sensor. A captured point cloud is divided into five layers based on height information, and the division of detected objects into two groups, cones and others, has been made using classifiers available in MATLAB toolboxes. To separate the classified conical objects into four types used to mark the route, an algorithm for their recognition was developed and used. The proposed solution, verified by navigation experiments in real conditions using an unmanned racing car, has gave good results, i.e., a high rate of cone-shaped objects classification, a short processing time and a low computational load. The performed tests have allowed also to diagnose the causes of incorrect classification of objects. Thus, the experimental results indicated that the approach presented in this work can be used in real time for autonomous, collision-free driving along marked routes.

Dual cytokine release from microsphere-containing decellularized extracellular matrix immune regulation promotes bone repair and regeneration

Most bone filler materials currently achieve bone regeneration by mimicking the natural bone extracellular matrix. However, it is difficult for these materials to replicate the structural functions and bioactivities, including immunomodulation, of natural bone perfectly to reduce inflammation and promote bone regeneration synergistically. Repairing bone defects with scaffolds using a decellularised extracellular matrix (dECM) as a matrix material is an important clinical application and research direction. Here, we processed bovine cancellous bone via an optimised combination of decellularisation methods and preferred dECM, which has the shortest processing time and lowest immunogenicity. Hexagonal mesoporous silica (HMS)/poly(lactic-co-glycolic acid) (PLGA) microspheres loaded with bone morphogenetic protein-2 (BMP-2) were prepared using the complex emulsion method. The HMS/PLGA microspheres had longer cytokine release periods than did the separate HMS and PLGA microspheres. Composite BMP-2/HMS/PLGA microspheres were used to prepare dual-loaded cytokine scaffolds with bone immunomodulatory capacity, which were prepared from composite BMP-2/HMS/PLGA microspheres to increase the osteogenic activity of the dECM and to adsorb interleukin-4 (IL-4) on the surface of the scaffolds. The results showed that the dECM had good cytocompatibility and mechanical strength, and the composite microspheres and IL-4 further endowed the dECM with an ordered spatiotemporally controlled release function, which could release BMP-2 for more than 4 months in the long term and release IL-4 for approximately 10 days in the short term. The composite scaffold not only effectively promoted the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) but also immunomodulated the M1 to M2 polarisation of macrophages (MPs) and mediated the M2 polarisation of MPs, which in turn promoted the osteogenic differentiation of BMSCs, creating a favourable immune microenvironment for bone regeneration. In vivo, the dual drug-loaded scaffolds also exhibited good biocompatibility and significantly superior immunomodulatory bone-enhancing properties compared with those of the other groups. In summary, the combination of dECM scaffolds with cytokine-carrying microspheres and immunomodulatory factors can promote the orderly spatiotemporal release of cytokines, which significantly enhances the bone regeneration and repair effects of dECM scaffolds and is a promising bone filler material for clinical application.

System Design, Modelling, Energy Analysis, and Industrial Applications of Ohmic Heating Technology

AbstractThe food and drink industry is gradually gaining sustainability pressure due to the high carbon emissions rate and limited availability of traditional energy supplies. Thus, it is essential to consider energy efficiency and decarbonisation of the ever-expanding food and drink industry. Ohmic heating technology is a highly energy-efficient and quick method of electrically heating food products. It uses the resistance of the food material to convert electrical energy into heat. However, the heating rate of ohmic heating depends on several process variables, such as electrical conductivity, voltage gradient, and voltage frequency. Therefore, it is important to study and understand the effects of these process variables to optimise the modelling and control of ohmic heating systems to obtain desirable output from food products. Hence, this study presents a review of the effects of process parameters on nutritional and organoleptic qualities, product yields, and energy efficiency of ohmic heating, along with its various industrial applications. The technology shows promising advancements in colour and nutritional quality, increasing energy efficiency, shorter processing times, and higher product yields compared to conventional heating processes.

Sparse-TFM imaging in frequency domain based on amplitude-squared parameter

Abstract The total focusing method (TFM) implemented in time domain has high complexity, limiting its large-scale engineering applications. In contrast, the frequency-domain TFM based on phase shift migration (PSM) is helpful to improve imaging efficiency. On this basis, partial data can be abandoned for imaging to save computing costs and shorten processing time without reducing imaging quality. In this paper, an adaptive sparse-TFM in the frequency domain is proposed by selecting desired signals from full matrix capture (FMC) data. The amplitude-squared parameter for all the received signals corresponding to each transmitting element is taken as the relevant weight, and sparse arrays are constructed adaptively according to the descending order of numerical values. Simulated and experimental results indicate that the proposed method has stable imaging quality under different sparsity ratios, rapidly improving imaging efficiency with good resolution for defect detection.

Adsorption of cadmium from wastewater with activated carbons derived from pig fur biowaste: A comparative study of in-situ and ex-situ activation routes

The environmental challenges associated with cadmium contamination in wastewater have necessitated the development of high-performing activated carbons (ACs) for effective wastewater treatment. Adsorption capacity depends on both the surface area and the adsorption-active functional groups developed on the adsorbent's surface during activation. Proper manipulation of key process variables using the appropriate activation route produces highly efficient and economically viable ACs. This research investigates the viability of pig fur biowaste as a novel precursor for activated carbons using two distinct activation methods—in-situ and ex-situ. Using a central composite design (CCD) of the Response Surface Methodology (RSM), the study systematically examines the effects of impregnation ratio, carbonization temperature, and carbonization time on the cadmium adsorption capacities of the resulting ACs. The optimal conditions for in-situ activation were found to be 691 °C, 175.11 min, and an impregnation ratio of 1.784 g/g, resulting in a cadmium adsorption capacity of 91.57 %. For ex-situ activation, the optimal conditions were 468.8 °C, 80.81 min, and an impregnation ratio of 2.915 g/g, which achieved a higher cadmium adsorption capacity of 91.21 %. Both types of activated carbons maintained high efficiency after five regeneration cycles, indicating they are suitable for long-term applications requiring repeated regeneration. Although both methods produced ACs with comparable cadmium removal efficiency, the ex-situ activation route proved to be more economically viable due to its lower temperature and shorter processing time. This study demonstrates the potential of pig fur biowaste as a sustainable and underutilized resource for AC production and highlights the ex-situ activation route as the more cost-effective approach for producing high-performance adsorbents.

Improving the synthesis of heterogeneous catalysts by gliding arc plasma using the “no-cooling system”

Glidarc plasma (GP) is a promising method for preparing heterogeneous catalysts due to its mild conditions and short processing time. This technique has been used to synthesize various catalysts like FeOx and MnOx. Adding a post-discharge (PD) step during synthesis has been shown to impart valuable new properties, such as phase transformation, increased specific surface area, and enhanced catalytic activity. Knowing that the PD step mostly impacts the plasma-synthesized solids maturation and can be accelerated if heat is introduced into the system, we now contemplate the possibility of accelerating the GP catalysts synthesis by allowing the solution to be heated by the energy spontaneously provided by the plasma without the necessity to add a PD. To challenge this approach, we subjected FeSO4 and SnSO4 precursors to plasma exposure without employing the cooling system usually integrated in the GP set-up and compared the resulting solids with those obtained via conventional GP synthesis involving a PD step. The findings show that for iron precursors, the absence of cooling accelerates the formation of non-catalytically active phases and causes particle agglomeration, which is undesirable. However, for tin precursors, the absence of a cooling system introduces features that further improve the catalytic activity of the solids. Precisely, tin oxide phases developed better than other less-catalytically active ones without affecting the textural properties of the solid. This suggests that in some cases, eliminating the PD step can simplify the GP catalyst synthesis process and reduce its environmental and economic impact.

Inactivation of Highly Pathogenic Avian Influenza Virus with High-temperature Short Time Continuous Flow Pasteurization and Virus Detection in Bulk Milk Tanks

Infections of dairy cattle with clade 2.3.4.4b H5N1 highly pathogenic avian influenza virus (HPAIV) were reported in March 2024 in the U.S. and viable virus was detected at high levels in raw milk from infected cows. This study aimed to determine the potential quantities of infectious HPAIV in raw milk in affected states where herds were confirmed positive by USDA for HPAIV (and therefore were not representative of the entire population), and to confirm that the commonly used continuous flow pasteurization using the FDA approved 72 °C (161°F) for 15 s conditions for high−temperature short time (HTST) processing, will inactivate the virus. Double-blinded raw milk samples from bulk storage tanks from farms (n = 275) were collected in four affected states. Samples were screened for influenza A using quantitative real-time RT-PCR (qrRT-PCR) of which 158 (57.5%) were positive and were subsequently quantified in embryonating chicken eggs. Thirty-nine qrRT-PCR positive samples (24.8%) were positive for infectious virus with a median titer of 3.5 log10 50% egg infectious doses (EID50) per mL. To closely simulate commercial milk pasteurization processing systems, a pilot-scale continuous flow pasteurizer was used to evaluate HPAIV inactivation in artificially contaminated raw milk using the most common legal conditions in the US: 72 °C (161°F) for 15 s. Among all replicates at two flow rates (n = 5 at 0.5 L/min; n = 4 at 1 L/min), no viable virus was detected. A mean reduction of ≥5.8 ± 0.2 log10 EID50/mL occurred during the heating phase where the milk is brought to 72.5 °C before the holding tube. Estimates from heat-transfer analysis support that standard U.S. continuous flow HTST pasteurization parameters will inactivate >12 log10 EID50/mL of HPAIV, which is ∼9 log10 EID50/mL greater than the median quantity of infectious virus detected in raw milk from bulk storage tank samples. These findings demonstrate that the US milk supply is safe when pasteurized.