Event Abstract Back to Event Immunostimulatory properties and antitumor activity of short double-stranded RNA against hepatocarcinoma G29 and melanoma B16 Tatiana Kabilova1*, Larisa Kovtonyuk1, Elena Ryabchikova1, Marina Zenkova1, Valentin Vlassov1, Elena Chernolovskaya1, Nelly Popova2 and Valeriy Nikolin2 1 Institute of Chemical Biology and Fundamental Medicine, SB RAS, Russia 2 Institute of Cytology and Genetics, SB RAS, Russia Short double-stranded RNAs containing specific sequences are able to activate the mammalian innate immune system. Here we identified a set of 19-bp RNA duplexes with 3-nucleotid overhangs in the 3’-ends with original sequence (here and after immunostimulating RNA, or isRNA), which has no significant homology with human mRNAs. The results in vitro experiments demonstrate that isRNAs under study efficiently inhibit growth of human cancer cells (KB-3-1, SK-N-MC), and induce synthesis of IFN-α and pro-inflammatory cytokines IL-6 and TNF-α in the human PBMCs. The examination of isRNAs sequence/activity relationships revealed that the introduction of substitutions in the middle part of the isRNA sequence does not alter the antiproliferative and immunostimulating activities, while substitutions in the 3’-end region of isRNA substantially reduce the activities. The results of in vivo experiments show that single intravenous injection of isRNA complexed with transfection reagent efficiently increases the level of IFN-α, and to a lesser degree the level of pro-inflammatory cytokine IL-6 in mice blood serum. We found that isRNA reduces the metastases area in the liver, kidneys and heart of CBA/LacSto mice with hepatocarcinoma G-29 and cause a slight, but reliable inhibition of the primary tumor growth. The results of experiments with C57BL mice with implanted melanoma B16 show that isRNA efficiently inhibits tumor growth (3.5–5-fold) and metastasis spreading in the lungs (5-fold). The obtained results revealed the pronounced immunostimulatory, anti-tumor and antimetastatic properties of the studied isRNAs. So, this short double stranded RNA can be considered as potential adjuvant for the therapy of oncological diseases. Acknowledgements This research was supported by the RAS programs Molecular and Cell Biology (no.22-1) and Science to Medicine (no.44), the Ministry of Science and Education of RF (no.14.740.11.1058), RFBR (no.11-04-01017-a) and SB RAS (no.85). Keywords: immunosimmunostimulatory RNAs, innate immunity, TLRs, Melanoma, hepatomaoma Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Innate immunity Citation: Kabilova T, Kovtonyuk L, Ryabchikova E, Zenkova M, Vlassov V, Chernolovskaya E, Popova N and Nikolin V (2013). Immunostimulatory properties and antitumor activity of short double-stranded RNA against hepatocarcinoma G29 and melanoma B16. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00656 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 13 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Tatiana Kabilova, Institute of Chemical Biology and Fundamental Medicine, SB RAS, Novosibirsk, Russia, kabilova@niboch.nsc.ru Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Tatiana Kabilova Larisa Kovtonyuk Elena Ryabchikova Marina Zenkova Valentin Vlassov Elena Chernolovskaya Nelly Popova Valeriy Nikolin Google Tatiana Kabilova Larisa Kovtonyuk Elena Ryabchikova Marina Zenkova Valentin Vlassov Elena Chernolovskaya Nelly Popova Valeriy Nikolin Google Scholar Tatiana Kabilova Larisa Kovtonyuk Elena Ryabchikova Marina Zenkova Valentin Vlassov Elena Chernolovskaya Nelly Popova Valeriy Nikolin PubMed Tatiana Kabilova Larisa Kovtonyuk Elena Ryabchikova Marina Zenkova Valentin Vlassov Elena Chernolovskaya Nelly Popova Valeriy Nikolin Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.
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